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fe-y AIR/WATER PARTITION COEFFICIENT ITEESSTSSUUBSTTAANNCCEE ~~~ =000000000 Identity: PFeCr-f9l5u.oro(o1c-tOacnteasnuelsfuolnfaotnei;c macaiyd,a1l,s1o,2b,e2,r3e,f3e.r4r,e4d,5t,o5a.6s.6P,7F,O7.S8,o8r,6- heptadecafiuoro-, potassium salt, CAS #2795-39-3) Remarks: The test substance is a white powder. No information was recorded on the purity. METHOD } Method: tThhies rvealisuenoforstraengdualradtiozryedpumreptohsoedso.loTghyeuesxepdertio mdeentterwmaisne designed by Dr. Richard Purdy of 3M's Environmental Laboratory and Don Mackay of D. Mackay Environmental Research Limited. GLP (Y/N): No Year completed: 1999 Remarks: The following method was devised and used: 1. Weigh approximately 0.01 gram of the test substance directly into a 2. Ttarraendsf2e5r02-0m0LmPLyreoxf"NAbNeaOkpeur.reRewcaotredr weight. into the beaker using a Class A giass volumetric pipet. 3. 2P5re0ppaLrealsioqluvoetntofbNlaAnNkOspaumrplee. Using a gas-tight water into a 25-mL syringe, Class A transfer glass. a volumetric flask partially filled with 50% methanol/ 50% ammonium aacmemtoatneibuumffaecrerteaatgeenbtu.ffeBrrirnegagteontv.olAummpeuwliatthe5i0n %anmaetmhbaenrolgl/a5ss0% autosampler vial. 4. Mix and sonicate the test substance in water sample (50 pg test substance/mL target nominal concentration) for approximately 10 minutes to ensure dissolution of the test material. 5. Prepare the control sample. Transfer a 250 uL aliquot of the test substance in water sample into a 25-mL Class A glass volumetric flask partially filled with 50% methanol / 50% ammonium acetate buffer reagent. Bring to volume with 50% methanol ammonium acetate buffer reagent. Ampulate in an / 50% amber glass autosampler vial. 000095 6. Pbrliancgestohleuttieosnt tsouabsbtoialn.ceAfitnewraatpeprrosxaimmpalteelbye1a0kemrLosn (a5h%o)tpolfawtaetaenrd hteamspeevraaptourraetiend,anreimceo-vweatbeerabkaethr.frTormanhsofteplractoenatnendtscooofl staomrpoloeminfo 7. a graduated cylinder and Process a 250 pL aliquot record actual of sample as volume. described above for solvent 8. bRleatnukmansdamcpolntertool samples. original beaker and bring sample to boil. 9. Repeat steps all ampulated 6-8 until samples sfaormpLlCeMhSasanealvyaspios.rated 10 100 mLs. Submit RESULTS TKeemiper0ature C: Not recorded. Remarks: "Don analytical results: Mackay provided the following interpretation of the "As | interpret concentration tofhe5l0abmgr/esLulints2t0h0eymeLstwaabtleirshtehdenandisitniitlilaeld off 10 mL apleirqcueonttsaagnedofantahleyozreidgitnahle treesstidsuueb.stTahnecye tphreensecnatlcwuhliacthedrtehmeained in ftrheombe1a3k6ertou1n0e5vawpiotrhalneod.realThtreesnde. `p|ecrcoenntacgeltrheuactodtvehereietse's;tranged substance did very sensitive mneottheovdapoofrmaeteastuorainnyg mleoawsauirra-wbalteerexptaerntti.tioTnhis is a coefficients. equally (e.g., Itthceancobnteesnthsowdno that not if water change ianncdomtphoessiotliuotne aesvawpoourladte osoclcuutre.witFhorawnataezre,olHroipsea)ptphroexniKmayteolryH2i4s00idePnati(caalppfroorxwiamtaetrelaynd the 20C) divided by 55000 molim or 0.044 Pa mmol or a Ka of about 2x 10. The test substance must thus have a Ka, cesosnesnitdiearlalyblnyonl-evsoslatthialne tfhriso,mi.ae.quleesosusthsaonlu2tioxn.10T.hisIt iiss pthruosb.ably becauseof its Kay of zero, ie. ionic is a ntaytpuer2e.inTvohleatsiliempclheemeixcpaeldiiennotuisr to assign it a nomenclature." DATA QUALITY _ wRealsianboiltitnyo:tedK.iimSitsucdhyrtaenmkpienrga2t.urDeawtaashansotlirmeictoerddreedli.ability. Sample purity 000096 cCoOnNcClLuUsSIiOoNnSs 00000 0 00000 Testing indicates solution. this substance is essentially non-volatile from aqueous Submitter: 3M Company, St. Paul, Minnesota, 55133 Environmental Laboratory, P.O. Box 33331, RREEFFEERREENCESS Study Ltd. of conducted at the request Easton, Maryland. of 3M Company by Wildife International, Study review Limited, by Don Mackay of D. Mackay Environmental Research OoTHR ER ____ Last changed: 5/1/00 000097 TWOT:ERNAC DaLleCBOae e RcRonESe PEOEnNDyEe aENC2E3/S. 3.M0 psec EROlaennta ShasmehcSoine015-10 CO: SGRiocenhnePuaery EEfapmr: LeabLa22bC35U2.0,30 5 FROM: SUSAN A. BEACH DATE: March 19,1990 `SUBJECT: Testing Results: Air-Water Partition Coefiicient (Ka) for PFOS WSRiedyedIeemTamteoaCnoapncctomynidnuctStnveadsessnetatund0ebsyomialahBootssarreteoonpaerntoen coonssicsect ((4Ce)us)oocrrPoPnRdEOuScS,,TagTsrhereeso8.n1 Sd onwithanya es Don i ea oSSASErIStuCrFrapnroceewdeSrnbaaetlreaupetRssaEtnOnoyeGCreataoaplsoiaeskdhpd aanTtheoaslpocaySonscoteanatoraetioonoomf0semssOdgSaShionee22C0m0o00kHmm1eU5Lu1Tn0wAr1eFt0E5pOehiSontpPiornoesesddteaenoaff Si Spent 0 BR 3 Koo eek Soa east 1 8Enent `a1Ptn0hed2cto0hinett]sesontlthuOsturesd.3oesKFnSoouetrntOcwiFhaat3laeln0ryg0,ne1oHni2-nivXscooal1pma0ptp.rio5ols.xeiitfmPiraootOnemalaySsqmuw2ueo4sou0tul0sdtPhSouaocslcu(uatairpvopwneri,ot3xhTihKaminaastwSeiCsleyoprr2o0oobCn)eabr)dlaiybvtbilhedeecIndao"Kubs.sy,tehoo5afn5rH0its0ii0sn.imidroceo.nmtniiu"csarleosfr.to0ro.wT0ash4te4exr Peas se the atached asic ests formore formation. Questons shoutbe directed fo RichPury. Regards, JN oo Lud, wn 000098 45] Wildlife sng + sania fae +x + 2% International aPAE Ltd. 8598 Commerce Drive Easton, Maryland 21601 3NET FAX TRANSMISSION this transTmisTsionIi ot received comreaaclnyaorif paggeessrareehiastiinng,Fpileeeasseocoalfl WWilidldife IinstmemaasttioonnalLLiddoant (441100)) 283225.805000 PLEASED From: NOTES: VERTO: RichPurdy 3M Corporation Scictist 3MEnvironmental Technology and Safety Services Raymond L. Van Hoven Rich: Enclosed you will find a completed data summary the PFOS air-water partition coefficient experiment including the thdaetafisnudminmgasr,yo,rtmoeptrhoovdidoeuatlninyea,dadnidtiionnsatlreuxmpeenrtiamlenctoandldiettiaoinlss.um1mlaoroyk.foPrlweaarsdetdoospneotakhiesnigtwatiethtoyocounitnactthmeneeatorfduitsucruses, 00099 reoSDsLEPaTsEiRrNcAoTInOsNAL LTD DpPurreaitnanam:itr risc1os3 Lspuempvetyotmne. PLwraisvaoeoi cevaomnnss SSnecoonnnsa v2T e ewE gm E rheeamm sI3omtdm 5 wpamemenn deem n bWoh dBe wmuemsme Bde ween ns prim, eee emonew ewee . bsweeoiiwn wim | Fee cm mmoeovomew san o os II ovr : CE mSeraz LSeerniee ! soissst anotsoiwast[[Vsimttoeeropet vio isms ]| nn Lise 1rsoonodeomo_ o B2 omy| QL 13.99 TswSetpieess CNo"onsGimma"itn N V mPak ee ar (VPaorm VB aem ae Deiren Voinye Amp"imolyRek hFetrAey a SSscooonoweml: umweeo 1o0 mvweewnm oaanee Soames a6. ma one HBZooo mmmaaaeee wweaonsw amwmmmsea Ho lmao mmo ams ee we SSSooollleaarss wGdeus n1ou ommmeesns aaonnmeee Soma me 5 mm om BHHooo dlmewuwoe mmmaminnm mpmsaannr Bo mew mem nam neeres ee cScoimaiens w bn n beewm oodm 3Bo mmaeww mmeanm nmueas eess fS e rommr o utse=tt -- htee ro me JF iem e ee cgoe t in cr ant inego nt OCFERTTHIEFIOERDICGOINPAYL ab en Origndeddof dojopak otpeach 454c-10) 000100 "Wildife International Lid -- METHODAOIURT-LWIANTEERFPOARRTTHIETIDOENTCEOREMFIFNIACTIIEONNT OF PFOS Project Number 454C-10 _-- 1. RWeeciogrhdswpepigphrto.ximately 0.01 grofaPFmOS (WIL#4675A) directlyino a tared 250-mL. Pyrex beaker 2. Transfer 200mLof Nanopure water into the beaker using a Class A glass volumetric pipet. 3. Pinrteopaar2e5s-olmvLenCtlabslsanAk sgalmapslsev.oluUmseitnrgicafglaasstkipgahrttisaylrliynfgiel,letdrawnistfher5a02%5m0eLthaanloilq/uoSt0o%faNmamnoonpiuruem water Aacmeptualteatbeufifneranreaagmebnet.r gBlrasisngautotovsoalmupmleerwviitahl50% methanol/S0% ammonium acetate buffer reagent. 4. aMpiprxoxainmdastoenliyca1t0emtihenuPtFesOStoiennswautreerdissasmopllueti(on5o0fugthPeFtOesSt/mmatLertiaarlg.et nominal concentration) for 5. CPlraespsarAe tghleascsonvtorloulmestamrpilcef.lasTkrapnarstfiearllay2f5il0leudLwiatlhiq5u0ot%ofmtehtehaPnFoOl/S50in%waamtemrosnaimupmleacienttaotea b2u5f-femrL armeabgeenrtg.laBsrsianugtotsoavmoplluemreviwailt.h 50% methanol/50% ammoni-um acetate buer reagent. Ampulate in an 6. P1l0amcLe sthe(5P%F)OoSfwiantwearthearssaemvpalpeorbaetaekd,erroenmoavheobtepalkateerfarndombhroitnpglsaotleutainodnctoooalbtiol.roAofmtetreamppperroaxtimuiartneelayn iocwater bath. Transfer contentsofsample into a graduated cyllnder and record actual volume. 7. Process 2.250 uLaliofqsuamoplte as described above for solvent blank and control samples, 8. Retum sample to original beaker and bring sample to boil 9.Raenpaelyastissteps 6-8 untilsamplehas evaporated to 100 mLs. Submit all ampulated samples for LCMS 000101 W-I_LDLIFE INTERNATIONAL LTD. LC/MS Operational Parameters for the Determination of PFOS Air-Water Partition Coefficient MASS SPECTROMETER: ION SOURCE: ION POLARITY: ION SOURCE VOLTAGE: ION SOURCE TEMPERATURE: ION MONITORED: LIQUID CHROMATOGRAPH: ANALYTICAL COLUMN: MOBILE PHASE: FLOW RATE: INJECTION VOLUME: RUN TIME: Perkin-Elmer Sciex API 100 LC Mass Spectrometer Perkin-Elmer Turbo lonspray Negative 5200 V 450C 498.6 amu `Hewlett-Packard 1090 Series IT Keystone Betacil C-18 (2 mm ID, 100 mm, 3 pm particle size) 65% methanol/35% ammonium acetate buffered water 240pL/min 10uL 8 minutes 000102 ~ re "b. Mackay Environmental Research Limited 1884 Champlain Drive Peterborough, Ontario Canada KsL 1N6 Tel (705) 740-2911 GST Reg. #R-101420263 3M991 "To: Dr. Rich Purdy, 3M Dr. Joyce SmithCooper, Battelle Dear Rich and Joye: `argerceeeimveendt twhieth"FoluuroreoxcpheecmtiactaiolnFsuagnadciwtey wMioldledloiongurRbeeqsutirteo mseatnitssfysytoautermneenetd.s.11Tshgeenperrinaclilpyalin ~ prergoabrldeimngwitlh,e mL osduseplecdte,vbeelogpamtehnetr,ing the input data. We can keep in close contact with you e`sWteabolbitsahiendeadnfirnoitmiaylocuontcheentPrLaOtiSonaiorf-w5a0temrg/pTa.ritinti2o0n0inmg Ldatwaa.teArstheinntdeirsptrieltletdhoeffab10remsuLltaslitqhueoyts raenmdaainnaeldysiendthtehebereaskiedrueu.nTchvaepyorttheedn.ca`lTchuelsaete"dpetrhecepnetrrceecnotvaegreoiefst"hreanogreidginfarloPm O13S6 tpore1s0e5ntwiwthhich vneoryreaslentsrietnidv.e mIectohnocdloudfomtehaastutrhienPgFJOowSaidri.dwnaotterevpaeproiirtaitoen tcooeaffniycimeenatss.uraIblceanexbteensth.owThnitshiastaif ``woactceurr waintdhathneaszoel0u(treoepvea)potrhaetneKeyqu,alolryHici.s itdheentciocnatlefnotrswdaotenrotancdhatnhgeesionlutceo.mpFoosritwiaontaesHrwoisuld Ka1p0ypy.rooxIftiamibasottuehtluys2e2xs4s0e1n00t"iP.aallP(yFanpOopSnr-ovmxouilmsaatttitleheluyfsr2h0omCaa)aqdvuKievoeyiudsedcsoobnlysuti5id5oen0r.a0b0Tlyhmilsoexisls tphroaornba0tb,hl0iy,44bicP.eaclmesa/smoutofhsalinteso2rxa cihoneimcicnaatluirne.ou"rThneosmicmupcllaeteaxrpee,dientisto assign it 8 Kuofzero, i. it i 8type 2 involatile `0Wfoe2c.fd4i,fdisseiadcmiapmtrleenestEh-iwQgaChteermrobodyfe4al.f8raucantnsodrucfsoiirshrna-egwsapKtoen,rdo(idnfigzmeetronsoti,hoeanlndedesnsps)airtotyi.fti1To0hn0e0c.odeelTgihrceaidednaittmsieoLnns/hikaoglnflfeoirsvsessopiawlre-twirateitoenr ~ alalt1s0yeearts. 1attach theLevelT, 1and ITresults wTihtehT2o0ve%liInrseosiull.ts show that PFOS haastrsung tendency to remain in water, i.e. typically 80% "The Level II results show an identical distribution. "The major loss by advectiion water with 000103 : B bos . only very slow degradation. The overall persistencein a refgscointroollned by the water The Level 11 results show that:- TFdischargteod air it will rapidlydeposit to soil (90%) and water (10%). Ifdischarged (0 its low sorption soilit tends tendency. toremainthere with the major loss beingrun-off to water because of If discharged (90%). towaterittends toremaininsolutionandissubject toremoval byadvestive flow "There is littl sorption to sediments. ~ Tn summary,itbehavesmuchas onewouldexpectofstrongly ionic compound. Tpgbeoerilhniagcptvsoercietoiimnseeiumtnphdoehreatralvnfetlrityvoescm.leoaIssneutsrchereurpteriaenlys.peanr1ttwirteoignoudnlecaotteoffrfiyitccilieisnmptaostsesfioubrlsieunbtcslotubasinoucnediwnhtdiheceghrma5oddasetolipoaenrnsdistent is weakness into the molecule? 1m conclusion, we 2 small irs step. are ready and willing to do modeling at your request. Please regard this as just Yours truly Don Mackay DM/um 000104