Document b97y0D6M0Jx2gzM3e7QX9qqk
IRC Summary
Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA
p. 1 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
cv-
RT (Exp RT) 1.26 (1.27) 3.59(3.60) 4.23 (4.26) 0.00(5.31) 6.23 (6.22) 6.31 (6.32) 1.26 (1.26) 5.30(5.30)
Area 93304 41237 5630
0 137320 18961 4107400 5157600
Modified?
Calc (Exp) MRM Ratio
No 1.00(1.00)
Ratio Confirms ID
YES
No 1.00(1.00)
YES
No 0.44 (0.40)
YES
No 0.00(0.10)
No VilL
No 8.01 (3.03)
No \ j /
No 0.34 (0.40)
YES
No 44.02(13.55)
No
No 78.79(13.06)
No
177
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Quantitative Peak Review
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K1000968-017
Before After
178
Page 203 of 245
..................................................
f l C o lu m b ia
cL j Analytical Services"
P- 3 Created with Analyst Reporter
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Ion Ratio Confirmation
1.26
1
PFBA2 213.0/169.0 Da Ci Baseline c Missed Wrong Peak
3.59
L .._j______
______ --
.0 i c, 1 JS
PFPeA2 263.0/219.0 Da Baseline Missed Wrong Peak
u > uwn
K1000968-017
. . 4.1 3..........
* *iuOtl ` OJH) l Wi ? in,,,,
PFBS2 299.0/99.0 Da G Baseline Missed Wrong Peak
PFHxA 2 313.0/119.0 Da D Baseline Misse Wrong Peak
Tfe imageisritk a ia i
PFHpA2 363.0/319.0 Da Baseline Missed a Wrong Peak
PFHxS2 399.0/99.0 Da Baseline o Missed a Wrong Peak
S_13C-PFDA 515.0/470.0 Da Baseline Missed o Wrong Peak
NB. PFBA and PFPeA do not have qualifying ion transitions.
Before After
N/A
Baseline Missed Wrong Peak
179
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P-4 Created with Analyst Reporter
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Project Data File Result Table Instrument Name
Ewan's Projects\PFCs 02081 Ob\SamplesLong.wiff 02081 OSamplesLong.rdb API 5000
Sample Name Acquisition Date Acquisition Method Injection Vial
K1000968-018 2/9/2010 2:19:16 AM PFCkinetexLong.dam 38
Injection Volume Sample Type Dilution Factor Weight to Volume
5 Unknown 1.00 0.00
Results Summary
Analyte Peak Name
IS Name
PFOA 1
13C-PFOA
PFNA 1
13C-PFNA
PFOS 1
13C-PFOS
PFDA 1
13C-PFDA
PFUnA 1
13C-PFUnA
PFDS 1
13C-PFOS
PFDoA 1
13C-PFDOA
PFTrA 1
13C-PFDOA
PFTeA 1
13C-PFDOA
IS Area 3740600 2531700 5534200 2563900 106220 5534200 127120 127120 127120
Analyte RT (Exp RT ) 1.39 (1.36) 2.34 (2.42) 2.39 (2.53) 3.32 (3.34) 4.13 (4.06) 4.13 (4.10) 4.76 (4.63) 5.29 (5.14) 5.74 (5.74)
Analyte Area 204510 121810 1379100 17747 10187 32080 11235 4872 4416
Calc. Cone (ng/mL) 1.160 0.444 2.350 0.095 0.080 0.070 0.128 0.072 0.132
Modified? No No No No No No No No No
180
I /L ( (tO Page 222 of 242
S erv ice s ''
p. 5 Created with Analyst Reporter
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IRC Summary
Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA 2 S_13C-PFDA PFUnA 2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2
RT (Exp RT ) 1.39(1.36) 1.39 (1.36) 2.33 (2.44) 2.35 (2.44) 2.39 (2.53) 2.37 (2.53) 0.00 (3.34) 3.35 (3.34) 4.12(4.06) 4.13 (4.06) 4.14 (4.10) 4.76 (4.64) 4.76 (4.64) 5.29(5.13) 5.75 (5.56)
Area 424560 3740600 35462 2531700 467800 5534200
0 2563900
3138 106220
9579 5337 127120 1608 1145
Modified?
Calc (Exp) MRM Ratio
No 2.08 (2.38)
Ratio Confirms ID
YES
No 18.29(50.17)
No
No 0.29(0.28)
YES
No 20.78(19.17)
YES
No 0.34 (0.32)
YES
No 4.01 (22.53)
No
No 0.00 (0.15)
No
No 144.47 (22.10)
No
No 0.31 (0.22)
No
No 10.43 (1.86)
No
No 0.30 (0.32)
YES
No 0.48 (0.29)
No
No 11.31 (3.08)
No
No 0.33(0.32)
YES
No 0.26 (0.36)
No
Page 223 of 242
181
yTC C olum bia * J Analytical S o rvico r
Quantitative Peak Review
P-6 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
K1000968-018
PFOA 1
413.0/169.0 Da
Baseline Missed n Wrnnn Peak
L_
i s :o
13C-PFOA 417.0/372.0 Da Baseline Missed n Wronn Peak
PFOS 1 499.0/80.0 Da
c Baseline Missed n Wrnnn Pnak 4^3
13C-PFOS 503.0/80.0 Da
Baseline Missed n Wrnnn Peak
PFNA 1 463.0/419.0 Da Baseline Missed n Wrnnn Pnak
Tmj inoi
PFDA 1 513.0/469.0 Da Baseline q Missed n Wrnnn Pnak
13C-PFNA 468.0/423.0 Da Baseline Missed
Wrnnn Peak
13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak
PFUnA 1 563.0/519.0 Da Baseline Missed ___n Wrnnn Peak___
L
13C-PFUnA 565.0/270.0 Da Baseline Missed ___ n Wrnnn Peak___
PFDS 1 599.0/80.0 Da Baseline Missed n Wrnnn Peak
13C-PFOS 503.0/80.0 Da u Baseline Missed
Wrnnn Peak
1
PFDoA 1 613.0/569.0 Da Baseline Missed n Wrnnn Pnak
5.74
13C-PFDOA
615.0/169.0 Da
d Baseline Missed Wrnnn Peak 4.76
PFTrA 1 663.0/619.0 Da Baseline Missed n Wrnnn Peak
13C-PFDOA 615.0/169.0 Da Baseline Missed ___ n Wrnnn Peak___
v X 'f A ^ Ml
PFTeAl 713.0/669.0 Da Baseline Missed n Wrnnn Peak
oi J'. l
13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Peak
Before After
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Ion Ratio Confirmation
' 1u ..
-1-39..............
irftsr
i
A --
W>X' 2m
___ _ _
__ _
PFOA2 413.0/369.0 Da c Baseline Missed Wrong Peak
PFNA2 463.0/219.0 Da Baseline Missed Wrong Peak
A JK1000968-018
i hU*J . / \ M .........
fr ViJ] I
M
"JlW[ - I ^
PFOS2 499.0/99.0 Da Baseline c Missed Wrong Peak
PFDA2 513.0/269.0 Da Baseline Missed Wrong Peak
PFUnA2 563.0/269.0 Da Baseline Missed Wrong Peak
PFDS2 599.0/99.0 Da Baseline Missed Wrong Peak
PFDoA2 613.0/169.0 Da Baseline Missed Wrong Peak
PFTrA 2 663.0/169.0 Da Baseline Missed Wrong Peak
PFTeA2 713.0/169.0 Da Baseline u Missed u Wrong Peak
Before After
183
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p. 8 Created with Analyst Reporter
Printed: 15/02/2010 2:34:00 PM
Project Data File Result Table Instrument Name
Ewan's Projects\PFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000
Sample Name Acquisition Date Acquisition Method Injection Vial
K1000968-018 2/9/2010 1:37:27 PM PFCkinetexShort.dam 38
Injection Volume Sample Type Dilution Factor Weight to Volume
5 Unknown 1.00 0.00
Results Summary
Analyte Peak Name
IS Name
PFBA 1
13C-PFBA
PFPeA 1
13C-PFHXA
PFBS 1
18 0 -P F H S
PFHxA 1
13C-PFHxA
PFHpA 1
13C-PFHxA
PFHxS 1
180-PFHS
S_180-PFHS
13C-PFDA
SJ3C-PFDA
13C-PFDA
IS Area 4953300 6510200 3540400 6510200 6510200 3540400 4648100 4648100
Analyte RT (Exp RT ) 1.27 (1.27) 3.58 (3.60) 4.25 (4.26) 5.30 (5.31) 6.21 (6.23) 6.31 (6.32) 6.31 (6.31) 7.64 (7.65)
Analyte Area 307700 585860 255860 550150 181330 956260 3540400 4648100
Calc. Cone (ng/mL) 0.53 0.92 0.22 0.73 0.66 0.80 0.86 1.00
Modified? No No No No No No No No
184
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Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
IRC Summary
Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA
RT (Exp RT ) 1.27(1.27) 3.58 (3.60) 4.25 (4.26) 5.30(5.31) 6.21 (6.22) 6.31 (6.32) 1.27(1.26) 5.30(5.30)
Area 307700 585860 90200 47999 521630 354890 4953300 6510200
Modified?
Calc (Exp) MRM Ratio
No 1.00(1.00)
Ratio Confirms ID
YES
No 1.00(1.00)
YES
No 0.35(0.40)
YES
No 0.09(0.10)
YES
No 2.88(3.03)
YES
No 0.37(0.40)
YES
No 16.10(13.55)
YES
No 11.83(13.06)
YES
185
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Quantitative Peak Review
p. 10 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
K1000968-018
Before After
186
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Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
Ion Ratio Confirmation
1.27
5.58
K1000968-018
._ -- .--
----
PFBA2 213.0/169.0 Da c Baseline Missed Wrong Peak
r 6.2i - -
PFPeA2 263.0/219.0 Da c Baseline Missed Wrong Peak
PFBS 2 299.0/99.0 Da Baseline Missed Wrong Peak
! <*,
-T 10*5
6.81............
; po* J bn;
i : i ......... 1 ............... `,5 (.0 5 70
PFHpA 2 363.0/319.0 Da c Baseline c Missed Wrong Peak
5b Ml 55
' ll
PFHxS2 399.0/99.0 Da Baseline Missed Wrong Peak
5', ill 85 .` 0
S 13C-PFDA 515.0/470.0 Da D Baseline o Missed Wrong Peak
NB. PFBA and PFPeA do not have qualifying ion transitions.
Before After
PFHxA 2 313.0/119.0 Da Baseline Missed Wrong Peak
Ihisimagesnotayaialale
N/A Baseline Missed
Wrong Peak
187
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p. 12 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
ft
Project Data File Result Table Instrument Name
Ewan's Projects\PFCs 02081 Ob\Samplesl_ong.wiff 02081 OSamplesLong.rdb API 5000
Sample Name Acquisition Date Acquisition Method Injection Vial
K1000968-019 2/9/2010 2:26:51 AM PFCkinetexLong.dam 39
Injection Volume Sample Type Dilution Factor Weight to Volume
5 Unknown 1.00 0.00
Results Summary
Analyte Peak Name
IS Name
PFOA 1
13C-PFOA
PFNA 1
13C-PFNA
PFOS 1
13C-PFOS
PFDA 1
13C-PFDA
PFUnA 1
13C-PFUnA
PFDS 1
13C-PFOS
PFDoA 1
13C-PFDOA
PFTrA 1
13C-PFDOA
PFTeA 1
13C-PFDOA
IS Area 3256900 2443100 5310000 2398200
92506 5310000 111670 111670 111670
Analyte RT (Exp RT ) 1.39 (1.36) 2.31 (2.42) 2.37 (2.53) 0.00(3.34) 4.11 (4.06) 0.00 (4.10) 0.00 (4.63) 0.00(5.14) 0.00(5.74)
Analyte Area 68424 22506 185890 0 3162 0 0 0 0
Calc. Cone (ng/mL) 0.467 0.104 0.343 N/A 0.014 N/A N/A N/A N/A
Modified? No No No No No No No No No
188
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ST Columbia Analytical Services"
p. 13 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
IRC Summary
Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS2 PFDoA2 S_13C-PFDOA PFTrA 2 PFTeA2
RT (Exp RT) 1.38(1.36) 1.39(1.36) 2.27 (2.44) 2.34 (2.44) 2.36 (2.53) 2.37 (2.53) 0.00 (3.34) 3.34(3.34) 0.00 (4.06) 4.13(4.06) 4.16(4.10) 4.75 (4.64) 4.76 (4.64) 5.26(5.13) 5.61 (5.56)
Area 122260 3256900
5849 2443100
64832 5310000
0 2398200
0 92506
257 1472 111670 383 272
Modified?
Calc (Exp) MRM Ratio
No 1.79 (2.38)
Ratio Confirms ID
No
No 47.60 (50.17)
YES
No 0.26 (0.28)
YES
No 108.55(19.17)
No
No 0.35 (0.32)
YES
No 28.57 (22.53)
No
No 0.00 (0.15)
No
No 0.00 (22.10)
No
No 0.00(0.22)
No
No 29.25(1.86)
No
No 0.00 (0.32)
No
No 0.00 (0.29)
No
No 0.00 (3.08)
No
No 0.00 (0.32)
No
No 0.00 (0.36)
No
189
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p. 14 Created with Anafyst Reporter
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Quantitative Peak Review
K1000968-019
1.39
; ....
1
; m
PFOA 1 413.0/169.0 Da Baseline o Missed n Wrnnn Peak
2.37
](-5 1.39
p
f I IVS
i os o ' : (i
13C-PFOA 417.0/372.0 Da L Baseline Missed n Wronn Peak
f lyuu
2.31
PFNA 1 463.0/419.0 Da o Baseline Missed n Wrnnn Peak
I'liOO
:mi5
5- ' *S
|
5 84 15
l :o
tn
13C-PFNA 468.0/423.0 Da Baseline Missed n Wronn Peak
5
,
i
1
f *5
I
.Ml
?5
3
;; loit) 500 .'5
*
i n.
i"y -:\r
J5
4fi
1 ;;;
I -------------------- ------------
PFOS 1 499.0/80.0 Da Baseline Missed n Wrnnn Peak
13C-PFOS
503.0/80.0 Da
Baseline Missed n Wrnnn Peak
4 13:uooo
PFDA 1 513.0/469.0 Da Baseline Missed n Wrnnn Peak
13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak
5e5
(M i- ,..0
i :<M
45
I3
1; r. 41] 45 'HI
l1000
i I! 1*5
:5 40 45 50
15 41 ] 4 5 5 0
I
30
i5
30
PFUnA 1 563.0/519.0 Da D Baseline Missed 1 1 Wrnnn Peak
? '*
I
13C-PFUnA 565.0/270.0 Da Baseline Missed n Wrnnn Peak 3fi#4 4.76
i ;r;
PFDS 1 599.0/80.0 Da a Baseline Missed n Wrnnn Peak
, 000 5,
13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Peak 33*4 4.76
:s*4
&
i :::
i,V '
COO 5 0*3
40 <5 S'] S1
<0 45 SO os
45 50 55 50
40 45 50 15
PFDoAl 613.0/569.0 Da Baseline Missed n Wrnnn Peak_____
r,no w.
5
13C-PFDoA
615.0/169.0 Da
Baseline Missed n Wrnnn Peak
m4 4.^6
35*4
PFTrA 1
13C-PFDOA
663.0/619.0 Da
615.0/169.0 Da
Baseline Missed
Baseline Missed
n Wrnnn Peak_____ _______n Wrnnn Peak_____
i:
i ::
__ .V i" v'
, 'V .
PFTeA 1 713.0/669.0 Da c Baseline Missed n Wrnnn Peak_____
JO 45
5A 55
Time nan
13C-PFDoA
615.0/169.0 Da
Baseline Missed
n Wrnnn Peak_____
Before After
190
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p. 15 Created with Analyst Reporter
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Ion Ratio Confirmation
i .... .1,38...........
. ..|..
1
l
A-
::
PFOA2 413.0/369.0 Da c Baseline u Missed Wrong Peak
. . . 2A .............
PFNA2 463.0/219.0 Da G Baseline Missed Wrong Peak
K1000968-019
... .. ./ .12/3 .
Wu# .n ....3.0...........
,-,.j
4 ,Mj
Z?,
-.0 J 3r)
40
PFOS 2 499.0/99.0 Da Baseline Missed Wrong Peak
PFDA 2 513.0/269.0 Da d Baseline Missed Wrong Peak
PFUnA 2 563.0/269.0 Da c Baseline Missed c Wrong Peak
4.16
PFDS2 599.0/99.0 Da Baseline Missed Wrong Peak
PFDoA2 613.0/169.0 Da Baseline Missed o Wrong Peak
PFTrA 2 663.0/169.0 Da c Baseline Missed Wrong Peak
PFTeA2 713.0/169.0 Da Baseline Missed c Wrong Peak
Before After
Page 229 of 242
191
p. 16 Created with Analyst Reporter
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Project Data File Result Table Instrument Name
Ewan's Projects\PFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000
Sample Name Acquisition Date Acquisition Method Injection Vial
K1000968-019 2/9/2010 1:46:59 PM PFCkinetexShort.dam 39
Injection Volume Sample Type Dilution Factor Weight to Volume
5 Unknown 1.00 0.00
Results Summary
Analyte Peak Name
IS Name
PFBA1
13C-PFBA
PFPeA 1
13C-PFHXA
PFBS 1
180-PFHS
PFHxA 1
13C-PFHXA
PFHpA 1
13C-PFHXA
PFHxS 1
180-PFHS
S J 8 0 -P F H S
13C-PFDA
SJ3C-PFDA
13C-PFDA
IS Area 4490700 5824900 3314600 5824900 5824900 3314600 4305000 4305000
Analyte RT (Exp RT ) 1.26 (1.27) 3.59 (3.60) 4.26 (4.26) 5.30 (5.31) 6.21 (6.23) 6.31 (6.32) 6.31 (6.31) 7.64 (7.65)
Analyte Area 272410 349050 112670 279050 67408 299650 3314600 4305000
Calc. Cone (ng/mL) 0.52 0.61 0.11 0.39 0.24 0.26 0.87 1.00
Modified? No No No No No No No No
192
V l^ L
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p. 17 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
IRC Summary
Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA
RT (Exp RT) 1.26(1.27) 3.59(3.60) 4.25(4.26) 5.30(5.31) 6.22 (6.22) 6.31 (6.32) 1.26 (1.26) 5.30 (5.30)
Area 272410 349050 43746 26809 220050 116480 4490700 5824900
Modified?
Calc (Exp) MRM Ratio
No 1.00(1.00)
Ratio Confirms ID
YES
No 1.00(1.00)
YES
No 0.39(0.40)
YES
No 0.10(0.10)
YES
No 3.26(3.03)
YES
No 0.39 (0.40)
YES
No 16.49 (13.55)
No
No 20.87(13.06)
No
193
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p. 18 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
Quantitative Peak Review
J 1.26
K1000968-019
5.30
l >4
f
:'
1 ..............
05 :IJI 15 CO
........ J
..............
10 1$ 4 45
46 6d l `S 0
PFBA T 213.0/169.0 Da Baseline Missed n Wronn Peak
13C-PFBA
217.0/172.0 Da c Baseline a Missed
n Wronn Peak i 6.31
PFPeAl 263.0/219.0 Da G Baseline Missed n Wronn Peak
IvH
13C-PFHXA
315.0/270.0 Da G Baseline Missed
n Wronn Peak 1`.rfi 5.^)
F frM. | 4lfcS
.5.5
40 45 5.i
55 60 65 ;0
.............. I
..........
45 50 55 60
PFBS 1 299.0/80.0 Da d Baseline Missed n Wronn Peak ,,-j 6.21
180-PFHS
403.0/103.0 Da
Baseline Missed n Wronn Peak 5f
PFHxA 1 313.0/269.0 Da O Baseline Missed n Wronn Peak
l7{*44 jl. 64**44
13C-PFHxA 315.0/270.0 Da Baseline d Missed 1fee n Wronn Peak 805
i t(leS
1 1 I ............... PFHpA 1
363.0/169.0 Da a Baseline Missed
n Wronn Peak
1i
S 180-PFHS 403.0/103.0 Da o Baseline Missed _____ n Wronn Peak
13C-PFHXA
315.0/270.0 Da
D Baseline Missed n Wrono Peak 7.f
56. 6!i I .5 '(I
PFHxsl 399.0/80.0 Da Baseline Missed n Wronn Peak
vj !t**1' f l. 50*5
.'P 75 90 95
13C-PFDA 515.0/470.0 Da Baseline Missed n-Wronn Peak_____
7U !I6 00 .15
S 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak_____
i 180-PFHS 403.0/103.0 Da Baseline Missed n Wronn Peak
7.^4
13C-PFDA 515.0/470.0 Da Baseline Missed n Wronn Peak_____
Before After
194
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S e rv ic e s 1
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Ion Ratio Confirmation
1.26
rJJ
3.59
I
K1000968-019
UUf'O 500____
4. 5
PFBA2 213.0/169.0 Da c Baseline Missed Wrong Peak
PFPeA2 263.0/219.0 Da Baseline Missed Wrong Peak
PFBS2 299.0/99.0 Da Baseline Missed Wrong Peak
" i 6.22
HI - --' ' * - --- - - - -..
t**
i_ V I 5 is*
| - 6;jjl - .......
A ll
PFHpA2 363.0/319.0 Da o Baseline Missed Wrong Peak
PFHXS2
399.0/99.0 Da
Q Baseline Missed Wrong Peak
Ii ^ II 55 6 0 66 Ml
S 13C-PFDA 515.0/470.0 Da Baseline Missed Wrong Peak
NB. PFBA and PFPeA do not have qualifying ion transitions.
PFHxA 2 313.0/119.0 Da Baseline Missed Wrong Peak
i M p Triable
N/A Baseline cj Missed
Wrong Peak
Before After
195
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p. 20 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
Project Data File Result Table Instrument Name
Ewan's ProjectsVPFCs 02081 Ob\SamplesLong.wiff 02081 OSamplesLong.rdb API 5000
Sample Name Acquisition Date Acquisition Method Injection Vial
K1000968-020 2/9/2010 2:34:26 AM PFCkinetexLong.dam 40
Injection Volume Sample Type Dilution Factor Weight to Volume
5 Unknown 1.00 0.00
Results Summary
Analyte Peak Name
IS Name
PFOA 1
13C-PFOA
PFNA 1
13C-PFNA
PFOS 1
13C-PFOS
PFDA 1
13C-PFDA
PFUnA 1
13C-PFUnA
PFDS 1
13C-PFOS
PFDoA 1
13C-PFDOA
PFTrA 1
13C-PFDOA
PFTeA 1
13C-PFDOA
IS Area 3251600 2544000 5931600 2174000 141010 5931600 119780 119780 119780
Analyte RT (Exp RT) 0.00(1.36) 0.00 (2.42) 2.33 (2.53) 0.00(3.34) 4.10 (4.06) 0.00 (4.10) 0.00 (4.63) 0.00(5.14) 0.00(5.74)
Analyte Area 0 0
14382 0
4916 0 0 0 0
Calc. Cone (ng/mL) N/A N/A 0.037 N/A 0.015 N/A N/A N/A N/A
Modified? No No No No No No No No No
196
Page 230 of 242
IRC Summary
Analyte Peak Name PFOA2 S__13C_PFOA PFNA2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2
ices
p. 21 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
RT (Exp RT) 0.00(1.36) 1.38(1.36) 2.39 (2.44) 2.33 (2.44) 2.34(2.53) 2.36 (2.53) 0.00 (3.34) 3.33(3.34) 0.00(4.06) 4.11 (4.06) 4.15(4.10) 4.74 (4.64) 4.74 (4.64) 5.27 (5.13) 5.73(5.56)
Area 0
3251600 425
2544000 5687
5931600 0
2174000 0
141010 432 588
119780 232 181
Modified?
Calc (Exp) MRM Ratio
No 0.00(2.38)
Ratio Confirms ID
No
No 0.00(50.17)
No
No 0.00(0.28)
No
No 0.00(19.17)
No
No 0.40(0.32)
YES
No 412.43 (22.53)
No
No 0.00(0.15)
No
No 0.00 (22.10)
No
No 0.00 (0.22)
No
No 28.68(1.86)
No
No 0.00 (0.32)
No
No 0.00(0.29)
No
No 0.00 (3.08)
No
No 0.00(0.32)
No
No 0.00(0.36)
No
197
Page 231 of 242
Anal
Quantitative Peak Review
p. 22 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
K1000968-020
PFOA 1 413.0/169.0 Da c Baseline Missed n Wrnnn Peak
13C-PFOA 417.0/372.0 Da o Baseline Missed n Wrnnn Peak___
V\
:n . s
PFOS 1
13C-PFOS
499.0/80.0 Da
503.0/80.0 Da
o Baseline a Missed
Baseline Missed
Wrnnn Peak
;nnrj 4.11
4.10 i
irni
fl i
PFUnA 1
F 'VH fj 1
-
`j0
I
1 1s Tmi(i1e1inn 13C-PFUnA
563.0/519.0 Da
565.0/270.0 Da
Baseline c Missed
Baseline Missed
n Wrnnn Peak
Wrnnn Peak
PFNA 1 463.0/419.0 Da Baseline Missed n Wrnnn Peak
;i\ ,,,r, ,'W
PFDA 1 513.0/469.0 Da Baseline o Missed n Wrnnn Peak
PFDS 1 599.0/80.0 Da Baseline Missed n Wrnnn Peak
13C-PFNA 468.0/423.0 Da Baseline Missed ___n Wrnnn Peak___
3.33
i 13C-PFDA 515.0/470.0 Da Baseline Missed i Wrnnn Peak
13C-PFOS 503.0/80.0 Da d Baseline Missed
Wrnnn Peak
k jk i,, j V '-.'v . s M
PFDoA 1 613.0/569.0 Da Baseline o Missed n Wrnnn Pnak
I_
13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Pnak
PFTrA 1 663.0/619.0 Da Baseline Missed ___n W rnnn Peak___
13C-PFDOA 615.0/169.0 Da Baseline Missed ___n Wrnnn Peak___
PFTeA 1 713.0/669.0 Da Baseline Missed i :Wrnnn Peak__ _
13C-PFDOA 615.0/169.0 Da c Baseline Missed n Wrnnn Peak
Before After
198
Page 232 of 242
Ion Ratio Confirmation
p. 23 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
K1000968-020
PFOA2 413.0/369.0 Da Baseline Missed a Wrong Peak
PFUnA2 563.0/269.0 Da Baseline Missed c Wrong Peak
PFNA2 463.0/219.0 Da Baseline o Missed Wrong Peak
PFDS 2 599.0/99.0 Da u Baseline Missed a Wrong Peak
\M PFOS 2
499.0/99.0 Da Baseline Missed
Wrong Peak
li&l
2. 'Ouu
2 sno
PFDoA2 613.0/169.0 Da Baseline a Missed Wrong Peak
PFDA2 513.0/269.0 Da Baseline Missed d Wrong Peak
vw Mil
i% m
I VHJ
UKI
4, S" ">$ l.ll
PFTrA2 663.0/169.0 Da Baseline Missed Wrong Peak
PFTeA2 713.0/169.0 Da Baseline Missed Wrong Peak
Before After
199
Page 233 of 242
p. 24 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
Project Data File Result Table Instrument Name
Ewan's Projects\PFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000
Sample Name Acquisition Date Acquisition Method Injection Vial
K1000968-020 2/9/2010 1:56:35 PM PFCkinetexShort.dam 40
Injection Volume Sample Type Dilution Factor Weight to Volume
5 Unknown 1.00 0.00
Results Summary
Analyte Peak Name
IS Name
PFBA 1
13C-PFBA
PFPeA 1
13C-PFHXA
PFBS 1
180-PFHS
PFHxA 1
13C-PFHxA
PFHpA 1
13C-PFHXA
PFHxS 1
180-PFHS
S J 8 0 -P F H S
13C-PFDA
SJ3C-PFDA
13C-PFDA
IS Area 5043500 5902900 3205200 5902900 5902900 3205200 4136100 4136100
Analyte RT (Exp RT ) 1.27 (1.27) 3.58 (3.60) 0.00 (4.26) 5.30 (5.31) 6.23 (6.23) 6.31 (6.32) 6.31 (6.31) 7.65 (7.65)
Analyte Area 48231 27194 0 53741 15090 34525
3205200 4136100
Calc. Cone (ng/mL) 0.03 0.03 N/A Cii'-L
0.03 0.01 \ / 0.02 0.88 1.00
Modified? No No No No No No No No
200
Page 213 of 245
p. 25 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
IRC Summary
Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA
RT (Exp RT ) 1.27(1.27) 3.58 (3.60) 0.00 (4.26) 0.00(5.31) 6.21 (6.22) 6.31 (6.32) 1.26(1.26) 5.30(5.30)
Area 48231 27194
0 0 133710 13767 5043500 5902900
Modified?
Calc (Exp) MRM Ratio
No 1.00(1.00)
Ratio Confirms ID
YES
No 1.00(1.00)
YES
No 0.00 (0.40)
No
No 0.00 (0.10)
NO I
No 8.86 (3.03)
No s K
No 0.40(0.40)
YES
No 104.57 (13.55)
No
No 109.84(13.06)
No
Page 214 of 245
201
Services"
Quantitative Peak Review
p. 26 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
K1000968-020
Before After
202
Page 215 of 245
Ion Ratio Confirmation
p. 27 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
K1000968-020
PFBA2 213.0/169.0 Da g Baseline Missed l Wrong Peak
6.?1
PFPeA2 263.0/219.0 Da Baseline o Missed Wrong Peak
PFBS2 299.0/99.0 Da Baseline Missed Wrong Peak
l
./ w
.
PFHpA2 363.0/319.0 Da c Baseline Missed o Wrong Peak
PFHxS2 399.0/99.0 Da Baseline Missed Wrong Peak
S_13C-PFDA 515.0/470.0 Da g Baseline a Missed Wrong Peak
NB. PFBA and PFPeA do not have qualifying ion transitions.
Before After
PFHxA 2 313.0/119.0 Da Baseline Missed Wrong Peak
Ife imageisriolrtble
N/A Baseline Missed
Wrong Peak
203
Page 216 of 245
Created with A n alyit Reporter Printed: 15/02/2010 2:34:00 PM
Project Data File Result Table Instrument Name
Ewan's Projects\PFCs 020910\Samplesshort.wiff 02081 OSamplesShortedit.rdb API 5000
Sample Name Acquisition Date Acquisition Method Injection Via!
KQ1000620-06 2/9/2010 5:24:58 PM PFCkinetexShort.dam 21
A|G>
Injection Volume Sample Type Dilution Factor Weight to Volume
5 Unknown 1.00 0.00
Results Summary
Analyte Peak Name
IS Name
PFBA 1
13C-PFBA
PFPeA 1
13C-PFHXA
PFBS 1
180-PFHS
PFHxA 1
13C-PFHXA
PFHpA 1
13C-PFHXA
PFHxS 1
180-PFHS
S_180-PFHS
13C-PFDA
S_13C-PFDA
13C-PFDA
IS Area 5889900 6654300 3729000 6654300 6654300 3729000 4230300 4230300
Analyte RT (ExpRT) 1.27 (1.27) 3.58 (3.60) 0.00 (4.26) 5.29 (5.31) 6.23 (6.23) 6.31 (6.32) 6.31 (6.31) 7.65 (7.65)
Analyte Area 74665 25091 0 60392 15261 40474
3729000 4230300
Calc. Cone (ng/mL) 0.06 0.02 N/A 0.03 0.00 0.02 1.00 1.00
Modified? No No No No No No No No
204
Page 241 of 245
ureatea witn Anaiy$Li20poner Printed: 15/02/2010 2:34:00 PM
IRC Summary
Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA
RT (Exp R T ) 1.27(1.27) 3.58(3.60) 0.00 (4.26) 0.00 (5.31) 6.21 (6.22) 6.31 (6.32) 1.26(1.26) 5.30(5.30)
Area 74665 25091
0 0 196620 17779 5889900 6654300
Modified?
Calc (Exp) MRM Ratio
No 1.00(1.00)
Ratio Confirms ID
YES
No 1.00 (1.00)
YES
No 0.00 (0.40)
No
No 0.00 (0.10)
No
No 12.88 (3.03)
No
No 0.44(0.40)
YES
No 78.88(13.55)
No
No 110.19(13.06)
No
205
Page 242 of 245
Quantitative Peak Review
Created with Analyst importer Printed: 15/02/2010 2:34:00 PM
KQ1000620-06
PFBA 1 213.0/169.0 Da Baseline Missed ___ n Wrono Peak
13C-PFBA 217.0/172.0 Da Baseline Missed n Wronn Peak
PFPeA 1 263.0/219.0 Da Baseline Missed n Wrnnn Peak
Sfl 15
130-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Peak
PFBS 1 299.0/80.0 Da Baseline Missed n Wrnnn Peak
180-PFHS 403.0/103.0 Da Baseline Missed
..Wrong Peak
Tun* mtr.
PFHxA 1 313.0/269.0 Da d Baseline Missed n Wrnnn Peak
13C-PFHXA 315.0/270.0 Da Baseline Missed n Wrnnn Peak
PFHpA 1 363.0/169.0 Da Baseline Missed n Wronn Peak
l(l6 6 .3 1
fi **S [ <0.5
S_180-PFHS 403.0/103.0 Da Baseline Missed ___ r Wrnnn Peak___
13C-PFHxA 315.0/270.0 Da Baseline c Missed n Wrnnn Peak
t 7.65
1 e*c
&
60-,
S 4M
Ld
PFHxS 1 399.0/80.0 Da Baseline Missed n Wrnnn Peak
13C-PFDA 515.0/470.0 Da Baseline Missed ___ n Wrnnn Peak___
SJ3C-PFDA 515.0/470.0 Da Baseline Missed ___ n Wrnnn Peak___
Before After
180-PFHS 403.0/103.0 Da Baseline Missed
Wronn Peak
13C-PFDA 515.0/470.0 Da Baseline Missed ___n W ronn Peak___
206
Page 243 of 245
Created with Analyft-ft&porter Printed: 15/02/2010 2:34:00 PM
Ion Ratio Confirmation
1 . 2 7 ..........................
Aj L
PFBA *2 213.0/169.0 Da Baseline Missed Wrong Peak
30e< :s*< |
1 . Of* 5 0*J
3 .5 8
jo H
5
PFPeA2 263.0/219.0 Da Baseline Missed Wrong Peak
KQ1000620-06
'SOO :aoo $ ,M
|i '.oeo wo
*0 5 50
PFBS2 299.0/99.0 Da Baseline Missed Wrong Peak
TOM
0000
* 00 J * * oo 2
?000-
1000
PFHxA 2 313.0/119.0 Da Baseline c Misse Wrong Peak
55
PFHpA2 363.0/319.0 Da Baseline Missed g Wrong Peak
PFHxS2 399.0/99.0 Da Baseline Missed Wrong Peak
SJ3C-PFDA 515.0/470.0 Da Baseline Missed Wrong Peak
NB. PFBA and PFPeA do not have qualifying ion transitions.
Before After
Ihisiiiageisnotsvible
N/A Baseline Missed
Wrong Peak
207
Page 244 of 245
Created with A n a l^ i^ e p o rte r Printed: 18/02/2010 3:24:00 PM
Project Data File Result Table Instrument Name
Ewan's Projects\PFCs 021710\SamplesLong.wiff 021710long.rdb API 5000
Sample Name Acquisition Date Acquisition Method Injection Vial
KQ1000620-06 2/17/2010 11:47:50 PM PFCkinetexLong.dam 21
<H0
Injection Volume Sample Type Dilution Factor Weight to Volume
5 Unknown 1.00 0.00
Results Summary
Analyte Peak Name
IS Name
PFOA 1
13C-PFOA
PFNA 1
13C-PFNA
PFOS 1
13C-PFOS
PFDA 1
13C-PFDA
PFUnA 1
13C-PFUnA
PFDS 1
13C-PFOS
PFDoA 1
13C-PFDOA
PFTrA 1
13C-PFDOA
PFTeA 1
13C-PFD0A
IS Area 3913000 3315700 9104600 2808200 190820 9104600 192430 192430 192430
Analyte RT (Exp RT ) 1.23 (1.36) 0.00 (2.06) 2.06 (2.09) 0.00(3.06) 3.90 (4.06) 3.87 (3.87) 4.58 (4.55) 5.27 (5.06) 0.00(5.51)
Analyte Area 3236 0
304670 0
9901
Calc. Cone (ng/mL) 0.045
N/A
0.328
N/A
0.018
-At?{itf i
,il\
-------- & 6 8 8 ~
2313
N/A
0 N/A
Modified? No No No No No No No No No
*
208
Page 166 of 170
Created with A n alylt Reporter Printed: 18/02/2010 3:24:00 PM
IRC Summary
Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2
RT (Exp RT ) 0.00(1.36) 1.25 (1.36) 2.03 (2.08) 0.00 (2.44) 2.11 (2.12) 2.09 (2.00) 3.11 (3.06) 3.08(3.04) 0.00 (3.88) 3.89(3.88) 0.00 (3.87) 0.00 (4.53) 4.53 (4.64) 5.26(5.13) 5.54(5.56)
Area 0
3913000 672 0
95436 9104600
744 2808200
0 190820
0 0 192430 182 127
Modified?
Calc (Exp) MRM Ratio
No 0.00 (2.16)
Ratio Confirms ID
No
No 1209.06 (43.19)
No
No 0.00 (0.34)
No
No 0.00 (0.00)
No
No 0.31 (0.32)
YES
No 29.88 (21.71)
No
No 0.00 (0.14)
No
No 0.00 (17.53)
No
No 0.00(0.19)
No
No 19.27(1.60)
No
No 0.00 (0.32)
No
No 0.00 (0.28)
No
No 20.62(3.71)
No.
No 0.08(0.34)
No
No 0.00 (0.29)
No
209
Page 167 of 170
Created with AnalJ%t^eporter Printed: 18/02/2010 3:24:00 PM
ItQuantitative Peak Review
KQ1000620-06
PFOA 1 413.0/169.0 Da Baseline c Missed n Wrnnn Peak
PFOS 1 499.0/80.0 Da Baseline Missed n Wrnnn Pp.ak
13C-PFOA 417.0/372.0 Da Baseline Missed
Wrnnn Peak
_ _2.09 k
13C-PFOS 503.0/80.0 Da Baseline Missed n Wronn Peak
PFNA 1 463.0/419.0 Da Baseline o Missed n Wrnnn Peak
PFDA 1 513.0/469.0 Da Baseline Missed n Wrnnn Peak
13C-PFNA 468.0/423.0 Da Baseline Missed n Wrnnn Peak
13C-PFDA 515.0/470.0 Da Baseline Missed
Wrnnn Peak
PFUnA 1 563.0/519.0 Da a Baseline Missed n Wrnnn Peak
13C-PFUnA 565.0/270.0 Da d Baseline Missed
i Wrnnn Peak
PFDS1 599.0/80.0 Da Baseline Missed n Wrnnn Peak
13C-PFOS 503.0/80.0 Da Baseline c Missed n Wrnnn Peak
PFDoA 1 613.0/569.0 Da Baseline Missed n Wrnnn Peak
13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Peak
!CMi
SOe) ] 5.27
30*6
PFTrA 1 663.0/619.0 Da Baseline Missed n Wrnnn Peak
13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Peak___
PFTeA 1 713.0/669.0 Da Baseline c Missed ___ n Wrnnn Peak___
13C-PFDOA 615.0/169.0 Da Baseline q Missed ___p W rnnn Peak___
Before After
210
Page 168 of 170
Created with AnalysfP-F^orter Printed: 18/02/2010 3:24:00 PM
Ion Ratio Confirmation
lyjiAi i />
5 i
lmifii il1-1
a> i !5 :o
3000 :sou 5 .'non if 5 S 000 *00
15 JO 25 30
PFOA2 413.0/369.0 Da Baseline c Missed Wrong Peak
PFNA2 463.0/219.0 Da Baseline Missed Wrong Peak
-000 1500/ X1 0000
snon
KQ1000620-06
T* mm
PFOS 2 499.0/99.0 Da Baseline Missi Wrong Peak
PFDA2 513.0/269.0 Da Baseline Missed Wrong Peak
- `0000 1X MW.
OH
:<** | 1SH .<
50*1
5.26
PFUnA 2 563.0/269.0 Da Baseline a Missed d Wrong Peak !0H
PFDS2 599.0/99.0 Da Baseline o Missed Wrong Peak
Tun nan
PFDoA2 613.0/169.0 Da Baseline c Missed Wrong Peak
PFTrA 2 663.0/169.0 Da Baseline a Missed c Wrong Peak
- :cv-t
i. 5.54 '.o tinssn.in in PFTeA2
713.0/169.0 Da Baseline a Missed
Wrong Peak
Before After
Page 169 of 170
211
p. 36 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
Project Data File Result Table Instrument Name
Ewan's Projects\PFCs 020910\Samplesshort.wiff 02081 OSamplesShortedit.rdb API 5000
Sample Name Acquisition Date Acquisition Method Injection Vial
KQ1000620-05 2/9/2010 5:15:26 PM PFCkinetexShort.dam 22
Injection Volume Sample Type Dilution Factor Weight to Volume
5 Unknown 1.00 0.00
Results Summary
Analyte Peak Name
IS Name
PFBA1
13C-PFBA
PFPeA 1
13C-PFHXA
PFBS 1
180-PFHS
PFHxA 1
130-PFHxA
PFHpA 1
130-PFHxA
PFHxS 1
180-PFHS
S J 8 0 -P F H S
13C-PFDA
SJ3C-PFDA
13C-PFDA
IS Area 5403500 6049600 3362100 6049600 6049600 3362100 3961000 3961000
Analyte RT (Exp RT ) 1.27 (1.27) 3.58 (3.60) 4.24 (4.26) 5.30 (5.31) 6.22 (6.23) 6.31 (6.32) 6.31 (6.31) 7.65 (7.65)
Analyte Area 11746000 11889000 19382000 11643000 4823600 19525000 3362100 3961000
Calc. Cone (ng/mL) 20.50 20.60 18.10 19.10 20.70 19.60 0.96 1.00
Modified? No No No No No No No No
212
>
-z, Page 237 of 245
Created with A n a ly ^ '^ p o rte r Printed: 15/02/2010 2:34:00 PM
IRC Summary
Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 SJ3C-PFBA S_13C-PFHxA
RT (Exp RT) 1.27(1.27) 3.58(3.60) 4.25 (4.26) 5.30(5.31) 6.22 (6.22) 6.31 (6.32) 1.26(1.26) 5.30 (5.30)
Area 11746000 11889000 7895400 1158200 13739000 8221600 5403500 6049600
Modified?
Calc (Exp) MRM Ratio
No 1.00(1.00)
Ratio Confirms ID
YES
No 1.00(1.00)
YES
No 0.41 (0.40)
YES
No 0.10(0.10)
YES
No 2.85 (3.03)
YES
No 0.42(0.40)
YES
No 0.46(13.55)
No
No 0.52 (13.06)
No
213
Page 238 of 245
Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
Quantitative Peak Review
KQ1000620-05
5 1.26
3.58
;o6
5.30
16*(
1 5P5
I
(1' >0
15
20
PFBA 7
213.0/169.0 Da
c Baseline Missed n Wrnnn Peak 4.24
:ti
1
is 45 Twne iui
PFBS 1
ia
299.0/80.0 Da
Baseline Missed n Wrnnn Peak
.4*C- 5.22
10*1
*
i0tt 1
55
5Ci I (.5
70
PFHpA~1
363.0/169.0 Da Baseline Missed
p Wrnnn Peak 6.31
& 6*5 *5
L
55 60 65 76
?
S -US 1 *4
C<5
1)5 10 15 20
13C-PFBA 217.0/172.0 Da c Baseline c Missed n Wrnnn Peak
M6
6 i t 1
I
55 60 65 70
180-PFHS 403.0/103.0 Da Baseline Missed n Wrnnn Peak
5.30
1S6
& 10C
S S0e5 45
1
50 55
0
13C-PFHxA
315.0/270.0 Da
Baseline Missed
n Wrnnn Peak
1 M.
06*6 2s
SO.S
1 40*5
7.^5
?0*S
& 15e$ .4I 10*6 S
50*5
I
30 35 Tn nan
PFPeA 1
40
i ^ 50*5
45
1
4.5 50 55
13C-PFHxA
6I>
263.0/219.0 Da
315.0/270.0 Da
Baseline Missed n Wrnnn Peak
)0*6
2Si
c Baseline Missed n Wrnnn Pm k
16*6
. ..0., | . 3 >0*6
& IU4C
!
60*5 5
I
50
55
PFHxA~1
60
I
45 50
55
Tun* min
13C-PFHXA
60
313.0/269.0 Da
315.0/270.0 Da
Baseline Missed
_________n Wrnnn .Peak_____ 6*6 6.^1
Baseline Missed
n Wrnnn Peak 6.31
8*6
9 3*5 51
16
55 SO (5 T*n* mki
PFHxS 1
4i 4.5
1 if5
70 85
i
6.0 65
180-PFHS
70
399.0/80.0 Da
403.0/103.0 Da
D Baseline a Missed n Wrnnn Peak
10*6 7.65
Q Baseline o Missed n Wrnnn Peak
l 0*6 7.65
*& 80*5 tik i
1
8(l*f
4& 60e5 | 40*5
20*5 20*5
70 76 00 <5
70 75 60 85
70 )S 0 85
S 180-PFHS 403.0/103.0 Da Baseline c Missed n Wrnnn Peak_____
13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak_____
S 13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak_____
13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak_____
Before After
214
Page 239 of 245
Ion Ratio Confirmation
Created with Ana Printed: 15/02/2010 2:34:00 PM
KQ1000620-05
PFBA2 213.0/169.0 Da o Baseline Missed Wrong Peak
PFPeA2 263.0/219.0 Da Baseline Missed a Wrong Peak
PFBS2 299.0/99.0 Da Baseline a Missed Wrong Peak
PFHxA 2 313.0/119.0 Da Baseline Missed Wrong Peak
PFHpA2 363.0/319.0 Da d Baseline Missed Wrong Peak
PFHxS2 399.0/99.0 Da Baseline Missed Wrong Peak
S_13C-PFDA 515.0/470.0 Da Baseline Missed Wrong Peak
NB. PFBA and PFPeA do not have qualifying ion transitions.
Before After
N/A
Baseline Missed Wrong Peak
215
Page 240 of 245
Columbia Analytical Services"
p. 40 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM
Project Data File Result Table Instrument Name
Ewan's Projects\PFCs 021710\SamplesLong.wiff 021710long.rdb API 5000
Sample Name Acquisition Date Acquisition Method Injection Vial
KQ1000620-05
VX- f
2/17/2010 11:40:18 PM
PFCkinetexLong .dam
22
Injection Volume Sample Type Dilution Factor Weight to Volume
5 Unknown 1.00 0.00
Results Summary
1Analyte Peak Name
IS Name
PFOA 1
13C-PFOA
PFNA 1
13C-PFNA
PFOS 1
13C-PFOS
PFDA 1
13C-PFDA
PFUnA 1
13C-PFUnA
PFDS 1
13C-PFOS
PFDoA 1
13C-PFDOA
PFTrA 1
13C-PFDOA
PFTeA 1
13C-PFDOA
IS Area 3601600 2912100 9040200 2873500 188040 9040200 177590 177590 177590
Analyte RT (ExpRT) 1.22 (1.36) 1.99 (2.06) 2.01 (2.09) 3.04 (3.06) 3.85 (4.06) 3.85 (3.87) 4.49 (4.55) 5.04 (5.06) 5.52 (5.51)
Analyte Area 3503400 6463900 19559000 6304500 4700500 14141000 3124100 1298700 1004800
Calc. Cone (ng/mL) 19.200 20.400 20.300 21.300 23.900 13.900 29.100 21.500 22.200
Modified? No No No No No No No No No
216
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IRC Summary
Analyte Peak Name PFOA 2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS2 PFDoA2 S_130-PFDoA PFTrA 2 PFTeA2
RT (Exp RT) 1.20(1.36) 1.23(1.36) 1.99 (2.08) 0.00 (2.44) 2.02 (2.12) 2.01 (2.00) 3.03 (3.06) 3.05(3.04) 3.85(3.88) 3.84 (3.88) 3.85 (3.87) 4.50 (4.53) 4.49 (4.64) 5.03(5.13) 5.51 (5.56)
Area 8128700 3601600 2125900
0 6235900 9040200 924920 2873500 955300 188040 4593600 776090 177590 399750 308040
Modified?
Calc (Exp) MRM Ratio
No 2.32(2.16)
Ratio Confirms ID
YES
No 1.03 (43.19)
No
No 0.33(0.34)
YES
No 0.00 (0.00)
No
No 0.32(0.32)
YES
No 0.46 (21.71)
No
No 0.15(0.14)
YES
No 0.46(17.53)
No
No 0.20(0.19)
YES
No 0.04 (1.60)
No
No 0.32(0.32)
YES
No 0.25 (0.28)
YES
No 0.06(3.71)
No
No 0.31 (0.34)
YES
No 0.31 (0.29)
YES
217
Page 163 of 170
ical m m m v
Quantitative Peak Review
|
1.23
Created with Anal^sfReporter Printed: 18/02/2010 3:24:00 PM
KQ1000620-05
PFOA 1 413.0/169.0 Da Baseline Missed
Wronn Poak
pfosT 499.0/80.0 Da Baseline Missed n Wrnnn Poak
1
13C-PFOA 417.0/372.0 Da Baseline Missed ___n Wronn Peak___
I
13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Ppak
PFNA 1 463.0/419.0 Da Baseline Missed r, Wrnnn Peak
2.5 30 15
PFDA 1 513.0/469.0 Da Baseline Missed
; Wrnnn Pp.ak
13C-PFNA 468.0/423.0 Da Baseline Missed
Wronn Peak
13C-PFDA 515.0/470.0 Da Baseline Missed
PFUnA 1 563.0/519.0 Da Baseline c Missed n Wronn Peak
13C-PFUnA 565.0/270.0 Da Baseline Missed n Wrnno Peak
Tin*, mi*
PFDS 1 599.0/80.0 Da Baseline Missed n Wronn Peak
13C-PFOS 503.0/80.0 Da Baseline Missed i Wronn Peak
PFDoA 1 613.0/569.0 Da Baseline Missed n Wronn Paak
130-PFDoA 615.0/169.0 Da Baseline Missed
Wronn Paak
50 55
PFTrA 1 663.0/619.0 Da Baseline Missed ____n Wrnnn Paak___
13C-PFDOA 615.0/169.0 Da Baseline Missed ___ n Wrnnn Peak___
PFTeA 1 713.0/669.0 Da o Baseline Missed ___ r Wronn Peak___
13C-PFDOA 615.0/169.0 Da c Baseline Missed ___n W ronn Peak___
Before After
218
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Ion Ratio Confirmation
KQ1000620-05
..........1 . 2 0 ................
.......... f II
(St'
A
s .f
..A..,,1
.. .... io\1S
ia1,, >Oei .......... M
...........
IS 20 25 10
15 20 25 50
Si
fc*$
8
2*5 1*5
PFOA2 413.0/369.0 Da d Baseline Missed g Wrong Peak
PFNA2 463.0/219.0 Da o Baseline Missed Wrong Peak
PFOS2 499.0/99.0 Da c Baseline d Missed c Wrong Peak
PFDA2 513.0/269.0 Da C Baseline Misse d Wrong Peak
PFUnA2 563.0/269.0 Da Baseline Missed g Wrong Peak
PFDS2 599.0/99.0 Da c Baseline Missed Wrong Peak
PFDoA2 613.0/169.0 Da c Baseline o Missed Wrong Peak
PFTrA2 663.0/169.0 Da Baseline Missed Wrong Peak
5 .3 1
G
PFTeA2 713.0/169.0 Da Baseline c Missed Wrong Peak
Before After
219
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Created with AnalyJt'Reporter Printed: 14/02/2010 6:41:00 PM
Project Data File Result Table Instrument Name
Sample Name Acquisition Date Acquisition Method Injection Vial
Ewan's Projects\PFCs 02081 Ob\Samplesl_ong.wiff 02081 OSamplesLong.rdb API 5000 p j He s -- / o
KQ1000620-03 2/9/2010 2:42:01 AM PFCkinetexLong.dam 67
Injection Volume Sample Type Dilution Factor Weight to Volume
5 Unknown 1.00 0.00
Results Summary
Analyte Peak Name
IS Name
PFOA 1
13C-PFOA
PFNA 1
13C-PFNA
PFOS 1
13C-PFOS
PFDA 1
13C-PFDA
PFUnA1
13C-PFUnA
PFDS 1
13C-PFOS
PFDoA 1
13C-PFDOA
PFTrA 1
13C-PFDOA
PFTeA1
13C-PFDoA
IS Area 3179000 2393400 6138100 2263400 116310 6138100 120990 120990 120990
Analyte RT (Exp RT ) 1.37 (1.36) 2.27 (2.42) 2.33 (2.53) 3.30 (3.34) 4.10 (4.06) 4.10 (4.10) 4.74 (4.63) 5.27 (5.14) 5.72 (5.74)
Analyte Area 3708600 5125100 19843000 3960600 2101400 7472600 1452500 862140 671680
Calc. Cone (ng/mL) 24.100 18.700 30.300 16.600 19.100 10.700 22.100 20.200 21.000
Modified? No No No No No No No No No
220
A{ o * '*
'7, , f ( < ?
Page 234 of 242
p. 45 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
IRC Summary
Analyte Peak Name PFOA 2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_130-PFDoA PFTrA 2 PFTeA2
RT (Exp R T ) 1.36(1.36) 1.35(1.36) 2.31 (2.44) 2.30 (2.44) 2.33 (2.53) 2.33 (2.53) 3.31 (3.34) 3.33(3.34) 4.10 (4.06) 4.10(4.06) 4.10 (4.10) 4.74 (4.64) 4 .7 4 (4.64) 5.26(5.13) 5.72 (5.56)
Area 8680100 3179000 1654700 2393400 6328000 6138100 616360 2263400 478180 116310 2415400 436550 120990 277810 228290
Modified?
Calc (Exp) MRM Ratio
No 2.34 (2.38)
Ratio Confirms ID
YES
No 0.86(50.17)
No
No 0.32(0.28)
YES
No 0.47(19.17)
No
No 0.32(0.32)
YES
No 0.31 (22.53)
No
No 0.16 (0.15)
YES
No 0.57 (22.10)
No
No 0.23 (0.22)
YES
No 0.06(1.86)
No
No 0.32(0.32)
YES
No 0.30(0.29)
YES
No 0.08(3.08)
No
No 0.32(0.32)
YES
No 0.34 (0.36)
YES
Page 235 of 242
221
./? C o lu m b ia
J Analytical Services"
Quantitative Peak Review
1.35
p. 46 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
KQ1000620-03
PFOA1
413.0/169.0 Da
o Baseline Missed n Wronn Peak ______
13C-PFOA 417.0/372.0 Da a Baseline Missed ___n Wronn Peak
PFNA 1 463.0/419.0 Da d Baseline Missed n Wrnnn Pnak
13C-PFNA 468.0/423.0 Da Baseline Missed n Wronn Peak
PFOS 1 499.0/80.0 Da Baseline Missed r Wrnnn Ppak
4.10
JL
PFUnA 1 563.0/519.0 Da g Baseline c Missed ___n Wronn Peak
13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Ppak
4.10
i 5 ft
ft.
13C-PFUnA 565.0/270.0 Da c Baseline Missed n Wrnnn Peak
4.74
PFDA 1 513.0/469.0 Da Baseline Missed
1 t*t 4.10
f 2<
.. i
1
ft
m
PFDS 1 599.0/80.0 Da Baseline q Missed ----- n Wrnnn Peak -- :o*i 5.^7
13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Ppak
2.33
< .rt
I .'r t
13C-PFOS 503.0/80.0 Da Baseline c Missed
Wrnnn Peak 16*4
8
PFDoA 1 613.0/569.0 Da o Baseline Missed n Wrnnn Peak
5.";
l
13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Ppak
PFTrA 1 663.0/619.0 Da Baseline Missed r Wrnnn Ppak
13C-PFDOA 615.0/169.0 Da G Baseline Missed o Wrnnn Ppak___
PFTeA 1 713.0/669.0 Da c Baseline Missed ___ n Wrnnn Peak
13C-PFDOA 615.0/169.0 Da Baseline Missed ___r W rnnn Pea k___
Before After
222
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r t C o lu m b ia A n a ly tic a l Services"
Ion Ratio Confirmation
1.36
D. 47 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
KQ1000620-03
PFOA 2 413.0/369.0 Da Baseline c Missed u Wrong Peak
PFNA2 463.0/219.0 Da Baseline Missed Wrong Peak
PFOS 2 499.0/99.0 Da Baseline Missed Wrong Peak
PFDA2 513.0/269.0 Da Baseline Missed q Wrong Peak
to
1
PFUnA 2 563.0/269.0 Da Baseline d Missed o Wrong Peak
PFDS 2 599.0/99.0 Da Baseline Missed Wrong Peak
4.;4
I
PFDoA2 613.0/169.0 Da Baseline Missed o Wrong Peak
5.26
PFTrA 2 663.0/169.0 Da d Baseline d Missed c Wrong Peak
PFTeA2 713.0/169.0 Da c Baseline o Missed Wrong Peak
Before After
223
Page 237 of 242
p. 48 Created with Analyst Reporter
Printed: 15/02/2010 2:34:00 PM
Project Data File Result Table Instrument Name
Ewan's Projects\PFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000
Sample Name Acquisition Date Acquisition Method Injection Vial
KQ1000620-03 2/9/2010 2:06:09 PM PFCkinetexShort.dam 67
Injection Volume Sample Type Dilution Factor Weight to Volume
5 Unknown 1.00 0.00
Results Summary
Analyte Peak Name
IS Name
PFBA 1
13C-PFBA
PFPeA 1
13C-PFHXA
PFBS 1
180-PFHS
PFHxA 1
130-PFHxA
PFHpA 1
13C-PFHXA
PFHxS 1
180-PFHS
S_180-PFHS
13C-PFDA
S_13C-PFDA
13C-PFDA
IS Area 4500600 5418600 3366800 5418600 5418600 3366800 4586500 4586500
Analyte RT (Exp RT ) 1.26 (1.27) 3.58 (3.60) 4.25 (4.26) 5.30 (5.31) 6.22 (6.23) 6.31 (6.32) 6.31 (6.31) 7.65 (7.65)
Analyte Area 9936200 11400000 21210000 12220000 4448200 23483000 3366800 4586500
Calc. Cone (rig/mL)
20.80 22.00 20.00 22.70 21.30 24.30 0.83 1.00
Modified? No No No No No No No No
224
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p. 49 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
' CK
IRC Summary
Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA
RT (Exp RT ) 1.26(1.27) 3.58(3.60) 4.25 (4.26) 5.30(5.31) 6.21 (6.22) 6.31 (6.32) 1.26(1.26) 5.30(5.30)
Area 9936200 11400000 8675600 1176500 13019000 10177000 4500600 5418600
Modified?
Calc (Exp) MRM Ratio
No 1.00(1.00)
Ratio Confirms ID
YES
No 1.00(1.00)
YES
No 0.41 (0.40)
YES
No 0.10(0.10)
YES
No 2.93(3.03)
YES
No 0.43(0.40)
YES
No 0.45 (13.55)
No
No 0.44(13.06)
No
225
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................................................. / X C o lu m b ia
O Analytical
Quantitative Peak Review
p. 50 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
KQ1000620-03
Before After
226
Page 219 of 245
/ ? C olum bia A nalytical Son/kos"
p. 51
Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
Ion Ratio Confirmation
1.26
3. 58'
PFBA2 213.0/169.0 Da Baseline d Missed Wrong Peak
k3
PFPeA2 263.0/219.0 Da Baseline Missed Wrong Peak
KQ1000620-03
4............. ,
PFBS2 299.0/99.0 Da c Baseline Missed Wrong Peak
PFHxA 2 313.0/119.0 Da Baseline Missed Wrong Peak
60 OS 7b
00*0
PFHpA2 363.0/319.0 Da Baseline Missed c Wrong Peak
PFHxS2 399.0/99.0 Da Baseline Missed Wrong Peak
S_13C-PFDA 515.0/470.0 Da o Baseline Missed Wrong Peak
NB. PFBA and PFPeA do not have qualifying ion transitions.
Before After
N/A
Baseline Missed Wrong Peak
227
Page 220 of 245
CfUiHf'itjfi
reivffcal Services"
Created with AnalystR^ifwrter Printed: 14/02/2010 6:41:00 PM
Project Data File Result Table Instrument Name
Sample Name Acquisition Date Acquisition Method Injection Vial
Ewan's Projects\PFCs 02081 OtASamplesLong.wiff 02081 OSamplesLong.rdb API 5000
([ etyj' - c/jC [)j
KQ1000620-04
i
2/9/2010 2:49:36 AM PFCkinetexLong.dam
68
Injection Volume Sample Type Dilution Factor Weight to Volume
5 Unknown 1.00 0.00
Results Summary
Analyte Peak Name
IS Name
PFOA 1
13C-PFOA
PFNA 1
13C-PFNA
PFOS 1
13C-PFOS
PFDA 1
13C-PFDA
PFUnA1
13C-PFUnA
PFDS 1
13C-PFOS
PFDoA1
13C-PFD0A
PFTrA 1
13C-PFDOA
PFTeA 1
13C-PFD0A
IS Area 3103900 2051400 4511700 2374100
75386 4511700
77328 77328 77328
Analyte RT (Exp R T) 1.39 (1.36) 2.34 (2.42) 2.35 (2.53) 3.32 (3.34) 4.09 (4.06) 4.10 (4.10) 4.73 (4.63) 5.26 (5.14) 5.71 (5.74)
Analyte Area 3677500 4273700 15682000 3291000 1673800 5610800 1167500 612880 345520
Calc. Cone (ng/mL) 24.400 18.200 32.600 13.100 23.500 11.000 27.800 22.500 16.900
Modified? No No No No No No No No No
228
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p. 53 Created with Analyst Reporter
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IRC Summary
Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA2 S_13C-PFDA PFUnA 2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2
RT (Exp RT ) 1.39(1.36) 1.37(1.36) 2.30 (2.44) 2.31 (2.44) 2.35 (2.53) 2.36 (2.53) 3.31 (3.34) 3.31 (3.34) 4.10(4.06) 4.10(4.06) 4.10(4.10) 4.73(4.64) 4.74 (4.64) 5.26 (5.13) 5.71 (5.56)
Area 8571100 3103900 1426000 2051400 5014100 4511700 497670 2374100 399530
75386 1781400 324640
77328 212670 123070
Modified?
Calc (Exp) MRM Ratio
No 2.33(2.38)
Ratio Confirms ID
YES
No 0.84(50.17)
No
No 0.33 (0.28)
YES
No 0.48(19.17)
No
No 0.32(0.32)
YES
No 0.29 (22.53)
No
No 0.15(0.15)
YES
No 0.72 (22.10)
No
No 0.24(0.22)
YES
No 0.05 (1.86)
No
No 0.32(0.32)
YES
No 0.28(0.29)
YES
No 0.07(3.08)
No
No 0.35 (0.32)
YES
No 0.36 (0.36)
YES
229
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Created with AnalyiV dporter Printed: 14/02/2010 6:41:00 PM
Quantitative Peak Review
! 1.39
Jwi
b t 15**> * 1U5
I1.37
I
IL
S
1 i*5
KQ1000620-04
2,34
B
\ ____ ft
PFOA 1 413.0/169.0 Da Baseline Missed p Wrnnn Pnak
2.35
13C-PFOA 417.0/372.0 Da Baseline Missed ___ n Wrnnn Peak___
PFNA 1 463.0/419.0 Da
Baseline Missed n Wrnnn Peak
13C-PFNA 468.0/423.0 Da Baseline Missed p Wrnnn Peak
PFOS 1 499.0/80.0 Da c Baseline a Missed n Wrnnn Peak
13C-PFOS 503.0/80.0 Da o Baseline Missed n Wrnnn Peak
4.10
PFDA 1 513.0/469.0 Da Baseline Missed
Wrnnn Peak
PFUnA 1 563.0/519.0 Da d Baseline Missed ___n Wrnnn Peak___
13C-PFUnA 565.0/270.0 Da Baseline Missed ___n Wrnnn Peak___
PFDS 1 599.0/80.0 Da o Baseline Missed n Wrnnn Peak
13C-PFDA
515.0/470.0 Da o Baseline Missed
n Wrnnn Peak
12.36
13C-PFOS
503.0/80.0 Da Baseline Missed
n Wrnnn Peak
PFDoA 1 613.0/569.0 Da q Baseline Missed n Wrnnn Peak
13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Peak
PFTrA 1 663.0/619.0 Da Baseline Missed n Wrnnn Peak___
45 Sfl
13C-PFDOA 615.0/169.0 Da Baseline Missed ___ p Wrnnn Peak
PFTeA 1 713.0/669.0 Da c Baseline Missed n Wrnnn Peak
13C-PFD0A 615.0/169.0 Da q Baseline Missed n Wrnnn Peak
Before After
230
Page 240 of 242
Colombia Analytical
p. 55 Created with Analyst Reporter
Printed: 14/02/2010 6:41:00 PM
Ion Ratio Confirmation
1.39
i 1j
2|3C|
m . i a t .............
PFOA 2 413.0/369.0 Da D Baseline Missed d Wrong Peak
PFNA2 463.0/219.0 Da d Baseline Missed o Wrong Peak
KQ1000620-04
r-M
i r j . 2j3$..............
zr\- / \ Mn .i --/.-.MMLV
In #
PFOS 2 499.0/99.0 Da Baseline o Missed Wrong Peak
? :! il
m:--1.......^...
.........
Tm nui.
PFDA2 513.0/269.0 Da c Baseline c Missed Wrong Peak
PFUnA2 563.0/269.0 Da q Baseline Missed d Wrong Peak
PFDS2 599.0/99.0 Da Baseline Missed Wrong Peak
PFDoA2 613.0/169.0 Da c Baseline Missed Wrong Peak
PFTrA 2 663.0/169.0 Da d Baseline Missed Wrong Peak
PFTeA2 713.0/169.0 Da c Baseline o Missed c Wrong Peak
Before After
Page 241 of 242
231
Columbia
A n alytical Services"
p. 56 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
Project Data File Result Table Instrument Name
Ewan's Projects\PFCs 020810\SamplesShort.wiff 02081 OSamplesShortedit.rdb API 5000
Sample Name Acquisition Date Acquisition Method Injection Vial
KQ1000620-04 2/9/2010 2:15:44 PM PFCkinetexShort.dam 68
Injection Volume Sample Type Dilution Factor Wejght to Volume
5 Unknown 1.00 0.00
Results Summary
Analyte Peak Name
IS Name
PFBA 1
13C-PFBA
PFPeA 1
13C-PFHXA
PFBS 1
180-PFHS
PFHxA 1
13C-PFHxA
PFHpA 1
13C-PFHxA
PFHxS1
180-PFHS
S_180-PFHS
13C-PFDA
S_13C-PFDA
13C-PFDA
IS Area 4259800 5160300 3066700 5160300 5160300 3066700 4359900 4359900
Analyte RT (Exp RT ) 1.26 (1.27) 3.58 (3.60) 4.25 (4.26) 5.30 (5.31) 6.22 (6.23) 6.31 (6.32) 6.31 (6.31) 7.65 (7.65)
Analyte Area 9880600 11205000 20699000 12208000 4378700 22943000 3066700 4359900
Calc. Cone (ng/mL) 21.90 22.70 21.60 23.90 22.00 26.50 0.80 1.00
Modified? No No No No No No No No
232
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p. 57 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
IRC Summary
Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA
RT (Exp RT) 1.26(1.27) 3.58 (3.60) 4.25 (4.26) 5.30(5.31) 6.22 (6.22) 6.31 (6.32) 1.26(1.26) 5.30(5.30)
Area 9880600 11205000 8785200 1157500 12493000 9897500 4259800 5160300
Modified?
Calc (Exp) MRM Ratio
No 1.00(1.00)
Ratio Confirms ID
YES
No 1.00(1.00)
YES
No 0.42 (0.40)
YES
No 0.09 (0.10)
YES
No 2.85(3.03)
YES
No 0.43(0.40)
YES
No 0.43 (13.55)
No
No 0.42(13.06)
No
233
Page 222 of 245
Quantitative Peak Review
p. 58 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
KQ1000620-04
Before After
234
Page 223 of 245
p. 59 Created with Analyst Reporter
P r in t e d : 1 5 / 0 2 / 2 0 1 0 2 : 3 4 : 0 0 PM
Ion Ratio Confirmation
` ' T.26............
tr: ..... i ................ us 10 15 ifl
?5i>
$& lSet ! ,0*6
5Of5
........3.58 ............
1i ...............
30 35 O
<5
PFBA2 213.0/169.0 Da Baseline Missed o Wrong Peak
PFPeA 2 263.0/219.0 Da Baseline Missed Wrong Peak
fi ??
KQ1000620-04
1........_ ............. 4 .
........-
I
35 4G 45 5D Tim* mm
PFBS2
"-I|
8
li i * |
S 1Oat -1
SOffj 00*0
45
5, k =
1 ........................ ....
50 55
5b
Tow <mn
PFHxA 2
299.0/99.0 Da
313.0/119.0 Da
Baseline Missed
Baseline Missed
Wrong Peak
Wrong Peak
1
PFHpA2 363.0/319.0 Da o Baseline Missed Wrong Peak
PFHxS2 399.0/99.0 Da Baseline q Missed Wrong Peak
S_13C-PFDA 515.0/470.0 Da Baseline Missed Wrong Peak
NB. PFBA and PFPeA do not have qualifying ion transitions.
Before After
'mageisnotviable
N/A Baseline Missed
d Wrong Peak
235
Page 224 of 245
p. 60 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
Project Data File Result Table Instrument Name
Ewan's Projects\PFCs 02091 OVSamplesshort.wiff 02081 OSamplesShortedit.rdb API 5000
Sample Name Acquisition Date Acquisition Method Injection Vial
KQ1000620-01 2/9/2010 4:56:19 PM PFCkinetexShort.dam 23
/{<, Injection Volume 5
Sample Type
Unknown
Dilution Factor
1.00
Weight to Volume 0.00
Results Summary
Analyte Peak Name
IS Name
PFBA 1
13C-PFBA
PFPeA 1
13C-PFHXA
PFBS 1
180-PFHS
PFHxA 1
13C-PFHXA
PFHpA 1
13C-PFHxA
PFHxS 1
180-PFHS
S_180-PFHS
13C-PFDA
SJ3C-PFDA
13C-PFDA
IS Area 4214700 4994800 3302300 4994800 4994800 3302300 4280400 4280400
Analyte RT (Exp RT ) 1.26 (1.27) 3.59 (3.60) 4.25 (4.26) 5.31 (5.31) 6.22 (6.23) 6.32 (6.32) 6.32 (6.31) 7.65 (7.65)
Analyte Area 10378000 13234000 23412000 14640000 5126300 32353000 3302300 4280400
Calc. Cone (ng/mL) 23.20 27.70 22.90 30.40 26.70 37.60 0.88 1.00
Modified? No No No No No No No No
236
A
"((o
Page 229 of 245
r J A n alytical Services"
p. 61 Created with Analyst Reporter Printed! 15/02/2010 2.34i00 PM
IRC Summary
Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA
RT (Exp RT) 1.26(1.27) 3.59 (3.60) 4.25 (4.26) 5.31 (5.31) 6.22 (6.22) 6.32 (6.32) 1.26(1.26) 5.31 (5.30)
Area 10378000 13234000 9933600 1471500 13804000 16038000 4214700 4994800
Modified?
Calc (Exp) MRM Ratio
No 1.00(1.00)
Ratio Confirms ID
YES
No 1.00(1.00)
YES
No 0.42(0.40)
YES
No 0.10(0.10)
YES
No 2.69(3.03)
YES
No 0.50(0.40)
YES
No 0.41 (13.55)
No
No 0.34 (13.06)
No
237
Page 230 of 245
Quantitative Peak Review
1.26
p. 62 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
KQ1000620-01
PFBA 1 213.0/169.0 Da Baseline Missed
4.25 | iI Jp
___ If1t ___ !
13C-PFBA
217.0/172.0 Da
Baseline Missed
n Wrnnn Peak
6.32
11^
"
I 1
r: ft
PFBS 1 299.0/80.0 Da Baseline Missed n Wrnnn Peak
180-PFHS 403.0/103.0 Da Baseline Missed n Wrnnn Peak
5,31
PFHpA 1 363.0/169.0 Da o Baseline Missed n Wrnnn Peak
S ' 60*5
ft
k
130-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Peak
PFPeA 1
13C-PFHXA
263.0/219.0 Da
315.0/270.0 Da
Baseline Missed
Baseline Missed
n Wrnnn Peak
5.31 126 10*6
___ 1___s*1| Ut
i I ft
i& 80*5 3 <UeS
;c6
S 65
PFHxA 1 313.0/269.0 Da d Baseline Missed
?*6 Oti ,, 5*6
1:
>5
6.32 1
ft
At
13C-PFHXA 315.0/270.0 Da Baseline Missed n Wrnnn Peak
ie 5
| 6*5 S' l
PFHxS 1 399.0/80.0 Da Baseline Missed n Wrnnn Peak
180-PFHS 403.0/103.0 Da d Baseline Missed n Wrnnn Peak
S J 8 0 -P F H S 403.0/103.0 Da a Baseline Missed n Wrnnn Peak___
13C-PFDA 515.0/470.0 Da Baseline o Missed n Wrnnn Peak
S_13C-PFDA 515.0/470.0 Da Baseline q Missed n Wrnnn Peak
Before After
13C-PFDA 515.0/470.0 Da Baseline Missed r. Wrnnn Peak___
238
Page 231 of 245
p. 63 Created with Analyst Reporter
Printed: 15/02/2010 2:34:00 PM
Ion Ratio Confirmation
.... 1;26
I
i !0
KQ1000620-01
PFBA2 213.0/169.0 Da Baseline Missed g Wrong Peak
PFPeA2 263.0/219.0 Da Baseline Missed Wrong Peak
PFBS2 299.0/99.0 Da o Baseline Missed Wrong Peak
~K2T I
to* 50*5 <0*5 ?0*5
PFHpA2 363.0/319.0 Da Baseline a Missed Wrong Peak
PFHxS2 399.0/99.0 Da Baseline Missed Wrong Peak
S_13C-PFDA 515.0/470.0 Da Baseline Missed Wrong Peak
NB. PFBA and PFPeA do not have qualifying ion transitions.
Before After
PFHxA 2 313.0/119.0 Da Baseline Missed Wrong Peak
N/A Baseline Missed
Wrong Peak
239
Page 232 of 245
p. 64 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM
it
Project Data File Result Table Instrument Name
Ewan's ProjectsVPFCs 021710\SamplesLong.wiff 021710long.rdb API 5000
Sample Name Acquisition Date Acquisition Method Injection Vial
KQ1000620-01 2/17/2010 11:25:11 PM PFCkinetexLong.dam 23
Injection Volume Sample Type Dilution Factor Weight to Volume
5 Unknown 1.00 0.00
Results Summary
Analyte Peak Name
IS Name
PFOA 1
13C-PFOA
PFNA 1
13C-PFNA
PFOS 1
13C-PFOS
PFDA 1
13C-PFDA
PFUnA 1
13C-PFUnA
PFDS 1
13C-PFOS
PFDoA 1
13C-PFDOA
PFTrA 1
13C-PFDOA
PFTeA 1
13C-PFDOA
IS Area 3208300 2508200 7403500 2971200 141100 7403500 140730 140730 140730
Analyte RT (Exp RT ) 1.24 (1.36) 2.00 (2.06) 2.06 (2.09) 3.08 (3.06) 3.84 (4.06) 3.86 (3.87) 4.49 (4.55) 5.03 (5.06) 5.50 (5.51)
Analyte Area 5749300 5838700 31342000 4804900 2980500 9878300 1926000 1015800 763940
Calc. Cone (ng/mL) 35.300 21.400 39.600 15.700 20.200 11.800 22.600 21.300 21.300
Modified? No No No No No No No No No
240
Page 154 of 170
Created with Analysf^eporter Printed: 18/02/2010 3:24:00 PM
IRC Summary
Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA 2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2
RT(ExpRT) 1.26(1.36) 1.24 (1.36) 2.02 (2.08) 0.00 (2.44) 2.05 (2.12) 2.04 (2.00) 3.04 (3.06) 3.04(3.04) 3.84 (3.88) 3.86 (3.88) 3.86 (3.87) 4.49 (4.53) 4.50 (4.64) 5.03 (5.13) 5.48 (5.56)
Area 12744000 3208300 1893900
0 10691000 7403500
700880 2971200 608500 141100 3242000 527580 140730 319340 278780
Modified? No
Calc (Exp) MRM Ratio
2.22(2.16)
Ratio Confirms ID
YES
No 0.56 (43.19)
No
No 0.32(0.34)
YES
No 0.00(0.00)
No
No 0.34 (0.32)
YES
No 0.24 (21.71)
No
No 0.15(0.14)
YES
No 0.62(17.53)
No
No 0.20(0.19)
YES
No 0.05(1.60)
No
No 0.33(0.32)
YES
No 0.27 (0.28)
YES
No 0.07 (3.71)
No
No 0.31 (0.34)
YES
No 0.36 (0.29)
No
Page 155 of 170
241
iumba
p. 66 Created with Analyst Reporter
Printed: 18/02/2010 3:24:00 PM
Quantitative Peak Review
KQ1000620-01
-V. i*5
2.00 20?
2[>,5
2 `Vj
& i (c
4 1| 23,,55
&
15?
1(1*6
1*5 1*5
1 i Ios n Timemisi ?o
l
05 ID 15 20
15 20 25 30
15 nf2l0nHBkh 25
PFOA 1
13C-PFOA
PFNA 7
13C-PFNA
413.0/169.0 Da
417.0/372.0 Da
463.0/419.0 Da
468.0/423.0 Da
d Baseline Missed 2Sr. n \A/rnnn Peak
8 15*4 | 1(lt(
`.(M
i r'j TO
2'.
PFOS1 499.0/80.0 Da Baseline Missed n \Afrnnn Ppak 5*5 3.84
4*5 & 1*5
S i'ei
1*5
S'. 40 45
PFUnA 1
Z Baseline d Missed n Wrnnn Peak
4*5
5*5
S S f 3*5 I ,4,
1*5
iti ts l 20 25
13C-PFOS
503.0/80.0 Da
Baseline o Missed
n Wrnnn Peak
:sok>
.'WOO
!&
15000 10QC0
Woo
I
50 35 40 45
13C-PFUnA
Baseline o Missed p Wrnnn Peak
5*5 3.08 5*5
i$ ^
| 3*5 5 2,5
1*5
30 25 30 35
pfdaT
513.0/469.0 Da
O Baseline Missed
n Wrnnn Peak
15*0
11V 10*0
I 50*5
3.86
1
50 30 35 Tim*m4i!0 45
PFDS 1
Baseline Missed 4*5 n Wrnnn Peak
5 & i1
40
I
n Ti3m0.nwt 25
13C-PFDA
515.0/470.0 Da
a Baseline o Missed
n Wrnnn Pp^k
5*5
4*5
5i
3=5
2?
1*5
2.04 15 k21* 25
13C-PFOS
in
563.0/519.0 Da
565.0/270.0 Da
599.0/80.0 Da
503.0/80.0 Da
Baseline Missed
c Baseline o Missed
Baseline Missed
Baseline Missed
n Wrnnn Peak
n Wrnnn Peak
4*5 4.49
30*4 4.50
:s*4
b & 00*4
I
1*5
13
10*4
1 _______
40 45 50
50*3
40
i45
50
n Wrnnn Peak 25*6
20*5 & 15,5. | 10*5
50*4
55 45 50 55
n Wrnnn Peak
30,4
4^25,4
ita
20*4 15,4
3 10*4
50,3
50 4# 45 50
55
PFDoXi 613.0/569.0 Da o Baseline Missed n Wrnnn Peak
5.50
13C-PFDOA
615.0/169.0 Da
Baseline Missed
p Wrnnn Peak 311*4 4j^)
:s*4 8 .-e*4 | '5*4 " 10*4
PFTrAI 663.0/619.0 Da Baseline Missed ______ n Wrnnn Peak_____
13C-PFDOA 615.0/169.0 Da Baseline Missed p Wrnnn Peak_____
A 40 5 5 55
PFTeA 1 713.0/669.0 Da Baseline o Missed n Wrnnn Peak_____
13C-PFDOA 615.0/169.0 Da
C Baseline Missed n Wrnnn Peak_____
Before After
242
Page 156 of 170
iiimbia Ion Ratio Confirmation
Created with AnalysPf&porter Printed: 18/02/2010 3:24:00 PM
KQ1000620-01
PFOA2 413.0/369.0 Da o Baseline Missed Wrong Peak
PFNA2 463.0/219.0 Da d Baseline Missed Wrong Peak
1S<-
.................. . . s i ,'4....................
L ........ ..... j j
PFUnA2 563.0/269.0 Da Baseline Missed Wrong Peak
t i 50*5
PFDS 2 599.0/99.0 Da Baseline Missed Wrong Peak
PFOS 2 499.0/99.0 Da Baseline c Missed Wrong Peak
PFDA2 513.0/269.0 Da Baseline Missed Wrong Peak
PFDoA2 613.0/169.0 Da Baseline Missed o Wrong Peak
1o*s
5CWM
OM
PFTrA 2 663.0/169.0 Da Baseline o Missed Wrong Peak
PFTeA2 713.0/169.0 Da Baseline Missed Wrong Peak
Before After
243
Page 157 of 170
p. 68 Created with Analyst Reporter
Printed: 15/02/2010 2:34:00 PM
Project Data File Result Table Instrument Name
Ewan's Projects\PFCs 020910\Sampiesshort.wiff 02081 OSamplesShortedit.rdb API 5000
Sample Name Acquisition Date Acquisition Method Injection Vial
KQ1000620-02
%% - | | f)A5
2/9/2010 5:05:54 PM
PFCkinetexShort.dam
24
Injection Volume Sample Type Dilution Factor Weight to Volume
5 Unknown 1.00 0.00
Results Summary
Analyte Peak Name
IS Name
PFBA 1
13C-PFBA
PFPeA 1
13C-PFHXA
PFBS 1
180-PFHS
PFHxA 1
130-PFHxA
PFHpA 1
13C-PFHXA
PFHxS1
180-PFHS
S_180-PFHS
13C-PFDA
SJ3C-PFDA
13C-PFDA
IS Area 4214300 5320100 3336400 5320100 5320100 3336400 4022000 4022000
Analyte RT (Exp RT ) 1.26 (1.27) 3.59 (3.60) 4.25 (4.26) 5.30 (5.31) 6.22 (6.23) 6.31 (6.32) 6.31 (6.31) 7.64 (7.65)
Analyte Area 10275000 13234000 23296000 14261000 4921800 32324000 3336400 4022000
Calc. Cone (ng/mL) 23.00 26.00 22.50 27.50 24.00 37.10 0.94 1.00
Modified? No No No No No No No No
244
^"4
(9 ^
^
f. i l o
Page 233 of 245
Created with Analy^'ffeporter Printed: 15/02/2010 2:34:00 PM
IRC Summary
Analyte Peak Name PFBA2 PFPeA2 PFBS2 PFHxA 2 PFHpA2 PFHxS2 S_13C-PFBA S_13C-PFHxA
RT (Exp RT) 1.26(1.27) 3.59(3.60) 4.25 (4.26) 5.30(5.31) 6.22 (6.22) 6.31 (6.32) 1.26(1.26) 5.30(5.30)
Area 10275000 13234000 9848700 1412600 13220000 16255000 4214300 5320100
Modified?
Calc (Exp) MRM Ratio
No 1.00(1.00)
Ratio Confirms ID
YES
No 1.00(1.00)
YES
No 0.42 (0.40)
YES
No 0.10(0.10)
YES
No 2.69(3.03)
YES
No 0.50(0.40)
No
No 0.41 (13.55)
No
No 0.37(13.06)
No
245
Page 234 of 245
Quantitative Peak Review
p. 70 Created with Analyst Reporter Printed: 15/02/2010 2:34:00 PM
KQ1000620-02
5.30
PFBA 1 213.0/169.0 Da Baseline Missed n Wrnnn Peak
13C-PFBA 217.0/172.0 Da Baseline Missed n Wrnnn Peak
PFPeA 1 263.0/219.0 Da Baseline Missed n Wrnnn Peak
13C-PFHxA 315.0/270.0 Da Baseline Missed n Wrnnn Ppak
PFBS 1 299.0/80.0 Da d Baseline Missed n Wrnnn Peak
60 i$
180-PFHS 403.0/103.0 Da Baseline Missed ___ r. Wrnnn Pnak___
5 SO S3 Tun min
PFHxA 1 313.0/269.0 Da Baseline Missed n Wrnnn Ppak
13C-PFHxA 315.0/270.0 Da o Baseline Missed n Wrnnn Peak
PFHpA 1 363.0/169.0 Da Baseline o Missed n Wrnnn Pftak
S_180-PFHS 403.0/103.0 Da Baseline Missed o Wrnnn Peak___
13C-PFHXA 315.0/270.0 Da o Baseline Missed n Wrnnn Peak
55 60 65 70
PFHxSI 399.0/80.0 Da Baseline Missed n Wrnnn Ppak
13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak___
Before After
S_13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak
180-PFHS 403.0/103.0 Da Baseline Missed n Wrnnn Pf-ak
8 0*5 6 0*S 0*6
7V 0
13C-PFDA 515.0/470.0 Da Baseline Missed ___ n Wrnnn Peak___
246
Page 235 of 245
Columbia
A nalytical
Ion Ratio Confirmation
Created with Analys?Reporter Printed: 15/02/2010 2:34:00 PM
KQ1000620-02
PFBA2 213.0/169.0 Da Baseline Missed Wrong Peak
PFPeA2 263.0/219.0 Da a Baseline Missed Wrong Peak
PFBS2 299.0/99.0 Da Baseline Missed Wrong Peak
os t(*i
5 6fl
J6.5 to*e
PFHpA2 363.0/319.0 Da Baseline Missed Wrong Peak
PFHxS2 399.0/99.0 Da c Baseline Missed Wrong Peak
S_13C-PFDA 515.0/470.0 Da Baseline c Missed Wrong Peak
NB. PFBA and PFPeA do not have qualifying ion transitions.
Before After
PFHxA 2 313.0/119.0 Da c Baseline Missed Wrong Peak
Thistiaqe isnotrtb le
N/A g Baseline Missed
Wrong Peak
247
Page 236 of 245
p. 72 Created with Analyst Reporter Printed: 18/02/2010 3:24:00 PM
Project Data File Result Table Instrument Name
Ewan's Projects\PFCs 021710\Samplesl_ong.wiff 021710long.rdb API 5000
Sample Name Acquisition Date Acquisition Method Injection Vial
KQ1000620-02 2/17/2010 11:32:43 PM PFCkinetexLong.dam 24
Injection Volume Sample Type Dilution Factor Weight to Volume
5 Unknown
1.00 0.00
Results Summary
Analyte Peak Name
IS Name
PFOA 1
13C-PFOA
PFNA 1
13C-PFNA
PFOS 1
13C-PFOS
PFDA 1
13C-PFDA
PFUnA 1
13C-PFUnA
PFDS 1
13C-PFOS
PFDoA 1
13C-PFDOA
PFTrA 1
13C-PFDOA
PFTeA 1
13C-PFDOA
IS Area 3101700 2471900 7509200 2881600 138680 7509200 148730 148730 148730
Analyte RT (ExpRT) 1.21 (1.36) 1.97 (2.06) 2.01 (2.09) 3.02 (3.06) 3.82 (4.06) 3.83 (3.87) 4.46 (4.55) 5.00 (5.06) 5.47 (5.51)
Analyte Area 6154700 5639900 33440000 5008800 3195800 10251000 2010700 931760 932890
Calc. Cone (ng/mL) 39.100
2 1 .0 0 0
41.700 16.800
2 2 .0 0 0 12.100
22.400 18.400 24.500
Modified? No No No No No No No No No
248
* -
Page 158 of 170
Created with Analy^'if^porter IB Printed: 18/02/2010 3:24:00 PM
IRC Summary
Analyte Peak Name PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA 2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2
RT (Exp RT ) 1.19(1.36) 1.19(1.36) 1.97 (2.08) 0.00(2.44) 1.99 (2.12) 2 .0 0 (2 .0 0 ) 3.01 (3.06) 3.00 (3.04) 3.81 (3.88) 3.83(3.88) 3.83 (3.87) 4.46 (4.53) 4.47 (4.64) 5.00 (5.13) 5.47 (5.56)
Area 12697000 3101700 1799000
0
11179000 7509200 730930 2881600 578150 138680 3330500 571530 148730 349410 340820
Modified?
Calc (Exp) MRM Ratio
No 2.06 (2.16)
Ratio Confirms ID
YES
No 0.50 (43.19)
No
No 0.32(0.34)
YES
No 0 .0 0 (0 .0 0 )
No
No 0.33(0.32)
YES
No 0.22(21.71)
No
No 0.15(0.14)
YES
No 0.58 (17.53)
No
No 0.18(0.19)
YES
No 0.04(1.60)
No
No 0.32 (0.32)
YES
No 0.28(0.28)
YES
No 0.07(3.71)
No
No 0.38 (0.34)
YES
No 0.37 (0.29)
No
249
Page 159 of 170
Services'
Quantitative Peak Review
Jl _ PFOA 1 413.0/169.0 Da c Baseline Missed
n Wrnnn Peak
13C-PFOA 417.0/372.0 Da Baseline Missed
Wrnnn Peak
PFOS 1 499.0/80.0 Da o Baseline Missed n Wrnnn Peak
13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Pnak
Created with Analps/^eporter Printed: 18/02/2010 3:24:00 PM
KQ1000620-02
15 2t Tim* tnn
PFNA1
25
463.0/419.0 Da
Baseline Missed n Wrnnn Peak
3.02
1S5
1
10*5 S
50*4
10
15 20
25
Tun, non
13C-PFNA
468.0/423.0 Da
Baseline Missed
n Wrnnn Peak 3.00
i
25 18 7*1*
PFDA 1
15
513.0/469.0 Da
Baseline Missed
n Wrnnn Peak
& 3t i
'5 4(1
i
25 10
35
13C-PFDA 515.0/470.0 Da o Baseline Missed n Wrnnn Peak
PFUnA 1 563.0/519.0 Da Baseline Missed n Wrnnn Peak
PFDoA 1 613.0/569.0 Da Baseline Missed
. '^5
c 1IMS
5.47
I
I
13C-PFUnA
PFDS 1
13C-PFOS
565.0/270.0 Da
599.0/80.0 Da
503.0/80.0 Da
Baseline d Missed n Wrnnn Peak
4.47
c Baseline Missed n Wrnnn Peak
:o 5.00
Baseline Missed Wrnnn Peak
30*4
25*4
1
<0 5 56 Tim*, mm
1 3C -P F D 0 A
a '** ''0*5
50*4
55
1
45 50
PFTrAI
55
& 3$l 15*4
1Q4 5 0*3
(0
13C-PFDoA
615.0/169.0 Da
663.0/619.0 Da
615.0/169.0 Da
Baseline Missed
n Wrnnn Peak
3 0*4
25*4
o Baseline Missed --..Wrnnn Peak_____
Baseline Misse n Wrnnn Peak
3 10*4
'.0 55 til
PFTeA 1 713.0/669.0 Da D Baseline Missed n Wrnnn Peak_____
13C-PFD0A 615.0/169.0 Da
Baseline Missed n Wrnnn Peak
Before After
250
Page 160 of 170
Ion Ratio Confirmation
Created with AnalysPffdfaorter Printed: 18/02/2010 3:24:00 PM
KQ1000620-02
Before After
Page 161 of 170
251
p. 76
Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
Analyte Name: Internal Standard:
PFOA 1 13C-PFOA
Data File Acquisition Date Acquisition Method Project
020810b\CalLong.wiff 2/8/2010 6:00:11 PM PFCkinetexLong.dam Ewan's Projects\PFCs
Result Table Algorithm Used Instrument Name
02081 OSamplesLong.rdb IntelliQuan API 5000
Regression Equation:________y = 0.0486 x + -0.00166 (r = Q.OOSQ)^
Exp Cone (ng/mL)
# of Values
Mean Calc Cone (ng/mL)
0.1 1 0.109
0.5 1
0.444
1 1 1.070
5 1 4.884
10 1 10.218
20 1 18.401
50 1 51.474
% Accuracy
109 89 107 98 102 92 103
/
STD
NaN NaN NaN NaN NaN NaN NaN
%CV
NaN NaN NaN NaN NaN NaN NaN
252
J<.
7 / i( C Page 1 of 242
p. 77
Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
Analyte Name: Internal Standard:
PFNA 1 13C-PFNA
Data File Acquisition Date Acquisition Method Project
02081 OtACalLong.wiff 2/8/2010 6:00:11 PM PFCkinetexLong .dam Ewan's Projects\PFCs
Result Table Algorithm Used Instrument Name
02081 OSamplesLong.rdb IntelliQuan API 5000
Regression Equation:
Exp Cone (ng/mL)
0.1 0.5
1 5 10 20 50
y = 0.115 x + -0.00273 (r= 0.9981)
# of Values
Mean Calc Cone (ng/mL)
1 0.109 1 0.467 1 0.975 1 4.961 1 10.803 1 17.941 1 51.343
% Accuracy
109 93 98 99 108 90 103
J
STD
NaN NaN NaN NaN NaN NaN NaN
%CV
NaN NaN NaN NaN NaN NaN NaN
.1
'.arv. /It
vT * *
''^7 7 -/
t
253
Page 2 of 242
jT i C o lu m b ia
J Analytical Services'
p. 78 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
Analyte Name: Internal Standard:
PFOS 1 13C-PFOS
Data File Acquisition Date Acquisition Method Project
Regression Equation:
Exp Cone (ng/mL)
0.1 0.5
1 5 10 20 50
020810b\CalLong.wiff 2/8/2010 6:00:11 PM PFCkinetexLong.dam Ewan's Projects\PFCs
Result Table Algorithm Used Instrument Name
02081 OSamplesLong.rdb IntelliQuan API 5000
y = 0.107 x + -0.00155 (r= 0.9990) /
# of Values
Mean Calc Cone (ng/mL)
1 0.089 1 0.488 1 1.059 1 5.162 1 10.967 1 18.843 1 49.992
% Accuracy
89 98 106 103 110 94 10p
STD
NaN NaN NaN NaN NaN NaN NaN
%CV
NaN NaN NaN NaN NaN NaN NaN
254
sj*
r'l-'X
( U ( ,D
Page 3 of 242
p. 79 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
Analyte Name: Internal Standard:
PFDA 1 13C-PFDA
Data File Acquisition Date Acquisition Method Project
Regression Equation:
Exp Cone (ng/mL)
0.1 0.5
1 5 10 20 50
02081 OtACalLong.wiff 2/8/2010 6:00:11 PM PFCkinetexLong.dam Ewan's Projects\PFCs
Result Table Algorithm Used Instrument Name
02081 OSamplesLong.rdb IntelliQuan API 5000
y = 0.106 x + -0.00306 (r = 0.9994) /
# of Values
Mean Calc Cone (ng/mL)
1 0.108 1 0.477 1 1.023 1 4.698 1 10.406 1 18.965 1 50.923
% Accuracy
108 95 102 94 104 95 102
STD
NaN NaN NaN NaN NaN NaN NaN
%CV
NaN NaN NaN NaN NaN NaN NaN
.v r' "'3 C'-
Js -V'
\7<-
r,
255
Page 4 of 242
j T i CbiliITlbi
Analytical
p. 80
Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
Analyte Name: Internal Standard:
PFUnA 1 13C-PFUnA
Data File Acquisition Date Acquisition Method Project
02081 OtACalLong.wiff 2/8/2010 6:00:11 PM PFCkinetexLong.dam Ewan's ProjectsVPFCs
Result Table Algorithm Used Instrument Name
02081 OSamplesLong.rdb IntelliQuan API 5000
Regression Equation:
Exp Cone (ng/mL)
0.1 0.5
1 5 10 20 50
y = 0.943 x + 0.0207 (r = 0.9983)
# of Values
Mean Calc Cone (ng/mL)
1 0.092 1 0.411 1 1.199 1 5.187 1 10.978 1 18.446 1 50.287
% Accuracy
92 82 120 104 110 92 101 /
STD
NaN NaN NaN NaN NaN NaN NaN
%CV
NaN NaN NaN NaN NaN NaN NaN
Page 5 of 242
256
/ ? Columbia : Analytical i'feii if J
p. 81 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
Analyte Name: Internal Standard:
PFDS 1 13C-PFOS
Data File Acquisition Date Acquisition Method Project
Regression Equation:
Exp Cone (ng/ml_)
0.1 0.5 1 5 10 20 50
02081 ObtCalLong.wiff
Result Table
02081 OSamplesLong.rdb
2/8/2010 6:00:11 PM
Algorithm Used
IntelliQuan
PFCkinetexLong.dam
Instrument Name API 5000
Ewan's Projects\PFCs
/
y = -0.000453 xA2 + 0.118x + -0.00243 (r = 0 .9 9 8 7 /
# of Values
Mean Calc Cone (ng/mL)
% Accuracy
STD
%CV
1 0.094
94 NaN NaN
1 0.490
98 NaN NaN
1 1.041 104 NaN NaN
1 5.064 101 NaN NaN
1 10.924 109 NaN NaN
1 18.516
93 NaN NaN
1 50.535 101 NaN NaN
257
4 Page 6 of 242
Analytical Services'
p. 82 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
Analyte Name: Internal Standard:
PFDoA 1 13C-PFDoA
Data File Acquisition Date Acquisition Method Project
Exp Cone (ng/mL)
0.1 0.5
1 5 10 20 50
020810b\CalLong.wiff 2/8/2010 6:00:11 PM PFCkinetexLong.dam Ewan's Projects\PFCs
Result Table Algorithm Used Instrument Name
02081 OSamplesLong.rdb IntelliQuan API 5000
/
# of Values
1 1 1 1 1 1 1
Mean Calc Cone (ng/mL)
0.082 0.518 0.985 5.302 11.377 19.829 48.506
% Accuracy
82 104 99 106 114 99 97
STD
NaN NaN NaN NaN NaN NaN NaN
%CV
NaN NaN NaN NaN NaN NaN NaN
> i |C
258
l I t * 1' 6 Page 7 of 242
Cointibia Analytical
p. 83 Createci with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
Analyte Name: Internal Standard:
PFTrA 1 13C-PFDoA
Data File Acquisition Date Acquisition Method Project
020810b\CalLong.wiff 2/8/2010 6:00:11 PM PFCkinetexLong.dam Ewan's Projects\PFCs
Result Table Algorithm Used Instrument Name
02081 OSamplesLong.rdb IntelliQuan API 5000
Regression Equation:________y = 0.352 x + 0.013 (r = 0.9967]
Exp Cone (ng/mL)
# of Values
Mean Calc Cone (ng/mL)
0.1 1 0.062 0.5 1 0.567
1 1 1.018 5 1 5.785 10 1 11.661 20 1 18.568 50 1 48.939
% Accuracy
62 113 102 116 117 93 98
STD
NaN NaN NaN NaN NaN NaN NaN
%CV
NaN NaN NaN NaN NaN NaN NaN
V'
259
Page 8 of 242
-> t i k \
p. 84 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
Analyte Name: Internal Standard:
PFTeA 1 13C-PFDoA
Data File Acquisition Date Acquisition Method Project
02081 OtACalLong.wiff 2/8/2010 6:00:11 PM PFCkinetexLong.dam Ewan's Projects\PFCs
Result Table Algorithm Used Instrument Name
02081 OSamplesLong.rdb IntelliQuan API 5000
Regression Equation:________y = -0.000306 xA2 + 0.27 x + -0.001 (r = 0.9948)
Exp Cone (ng/mL)
# of Values
Mean Calc Cone (ng/mL)
% Accuracy
0.1 1 0.080
80
0.5 1
0.587
117
1 1 0.928 93
5 1 5.191 104
10
1
11.943
119
20 1 17.075 85
50 1 50.817 102
STD
NaN NaN NaN NaN NaN NaN NaN
%CV
NaN NaN NaN NaN NaN NaN NaN
.. L *-*" ''_____________ ______ ___________ ______ ____________ __________________ __.________________
260
7 - IU- (0 Page 9 of 242
/ Columbia
* 3 Analytical Services''
p .85 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
Project Data File Result Table Instrument Name
Ewan's Projects\PFCs 02081 Ob\CalLong.wiff 02081 OSamplesLong.rdb API 5000
Sample Name Acquisition Date Acquisition Method Injection Vial
IB 2/8/2010 6:00:11 PM PFCkinetexLong.dam 1
Injection Volume Sample Type Dilution Factor Weight to Volume
5 Unknown 1.00 0.00
Results Summary
Analyte Peak Name
IS Name
PFOA 1
13C-PFOA
PFNA 1
13C-PFNA
PFOS 1
13C-PFOS
PFDA 1
13C-PFDA
PFUnA 1
13C-PFUnA
PFDS 1
13C-PFOS
PFDoA 1
13C-PFDOA
PFTrA 1
13C-PFDOA
PFTeA1
13C-PFDOA
IS Area 305 253 326 97 33 326 139 139 139
Analyte RT (Exp RT ) 0.00(1.36) 0.00 (2.42) 2.36 (2.53) 0.00 (3.34) 4.16 (4.06) 0.00(4.10) 0.00 (4.63) 0.00(5.14) 0.00(5.74)
Analyte Area 0 0
955 0
2031 0 0 0 0
Calc. Cone (ng/mL) N/A N/A 27.500 N/A 65.500 N/A N/A N/A N/A
Modified? No No No No No No No No No
Page 10 of 242
261
p. 86 Created with Analyst Reporter
C o l Printed: 14/02/2010 6:41:00 PM
IRC Summary
Analyte Peak Name PFOA 2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 S_13C-PFOS PFDA2 SJ3C-PFDA PFUnA2 S_13C-PFUnA PFDS2 PFDoA2 S_13C-PFD0A PFTrA 2 PFTeA2
RT (Exp RT) 0.00(1.36) 1.25(1.36) 2.25 (2.44) 2.48 (2.44) 2.29 (2.53) 2.77 (2.53) 0.00(3.34) 3.34 (3.34) 0.00 (4.06) 4.17 (4.06) 4.06 (4.10) 4.69 (4.64) 4.85 (4.64) 5.14(5.13) 5.59 (5.56)
Area 0
305 63 253 255 326 0 97 0 33 365 635 139 376 236
Modified?
Calc (Exp) MRM Ratio
No 0.00 (2.38)
Ratio Confirms ID
No
No 0.00 (50.17)
No
No 0.00 (0.28)
No
No 0.00(19.17)
No
No 0.27(0.32)
YES
No 0.34 (22.53)
No
No 0.00(0.15)
No
No 0.00 (22.10)
No
No 0.00 (0.22)
No
No 0.02(1.86)
No
No 0.00 (0.32)
No
No 0.00 (0.29)
No
No 0.00(3.08)
No
No 0.00 (0.32)
No
No 0.00 (0.36)
No
262
Page 11 of 242
es"
Quantitative Peak Review
Created with A nalyst^i^orter Printed: 14/02/2010 6:41:00 PM
IB
Before After
263
Page 12 of 242
Colum bia
Analytical
Ion Ratio Confirmation
p. 88 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
IB
PFOA2 413.0/369.0 Da c Baseline Missed a Wrong Peak
PFNA 2 463.0/219.0 Da Baseline Missed Wrong Peak
PFOS2 499.0/99.0 Da Baseline Missed Wrong Peak
PFDA2 513.0/269.0 Da Baseline Missed Wrong Peak
-rw rA r
PFUnA2 563.0/269.0 Da c Baseline Missed Wrong Peak
PFDS2 599.0/99.0 Da o Baseline o Missed Wrong Peak
PFDoA2 613.0/169.0 Da d Baseline o Missed Wrong Peak
PFTrA 2 663.0/169.0 Da d Baseline Missed o Wrong Peak
PFTeA2 713.0/169,0 Da Baseline u Missed q Wrong Peak
Before After
264
Page 13 of 242
ytcl Services
Created with Analyst B'e^&rter Printed: 14/02/2010 6 4 1 :00 PM
Project Data File Acquisition Method
Ewan's Projects\PFCs 02081 OtACalLong.wiff PFCkinetexLong.dam
Sample Name Acquisition Date Acquisition Method Sample ID Sample Comment
PFC 0.10ppb 2/8/2010 6:22:50 PM PFCkinetexLong.dam LCMS2-85A
Results Summary
Analyte Name
Analyte RT (Expected)
PFOA 1
1.36 (1.36)
IS Name 13C-PFOA
IS Area 2771900
PFNA 1
2.31 (2.42) 13C-PFNA 2300200
PFOS 1
2.35 (2.53) 13C-PFOS 6845000
PFDA 1
3.32 (3.34) 13C-PFDA 2198200
PFUnA 1 4.10(4.06) 13C-PFUnA 162500
PFDS 1
4.12(4.10) 13C-PFOS 6845000
PFDoA 1 4.76 (4.63) 13C-PFDOA 239910
PFTrA 1 5.29 (5.14) 13C-PFDoA 239910
PFTeA 1 5.74 (5.74) 13C-PFDOA 239910
Result Table Algorithm Used Instrument Name
02081 OSamplesLong.rdb IntelliQuan API 5000
Injection Vial Injection Volume Sample Type Dilution Factor Weight to Volume
2 5 Standard 1.00 0.00
Analyte Area 10143 22540 54526 18255 17391 59395 15176 8315 4924
Exp Cone (ng/mL)
0.100 0.100 0.100 0.100 0.100 0.100 0.100 0.100 0.100
Calc. Cone (ng/mL)
0.109
Accuracy (%)
109
0.109
109
0.089
89
0.108
108
0.092
92
0.094
94
0.082
82
0.062
62
0.080
80
Mod? No NO No No No No No No No
265
to Page 14 of 242
IRC Summary
i Analyte Peak Name
PFOA2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 S _13C -P F O S PFDA2 S_13C-PFDA PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2
p. 90
Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
RT (Expected)
1.33(1.36) 1.35(1.36) 2.32 (2.44) 2.28 (2,44) 2.33 (2.53) 2.32 (2.53) 3.33 (3.34) 3.32(3.34) 4 .1 0 (4 .0 6 ) 4 .1 0 (4 .0 6 ) 4.12(4.10) 4.75 (4.64) 4.76 (4.64) 5 .2 9 (5 .1 3 ) 5.75(5.56)
Area
19840 2771900
3319 2300200
16682 6845000
2768 2198200
2974 162500 17921
2899 239910
2346 1505
Modified?
No No No No No No No No No No No No No No No
Calculated Ion Ratio (Expected Value)
1.96 273.28
0.15 102.05
0.31 125.54
0.15 120.42
0.17 9.34 0.30 0.19 15.81 0.28 0.31
266
Page 15 of 242
p. 91 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
Quantitative Peak Review
!
1.35
1
PFOA 1 413.0/169.0 Da c Baseline Missed r Wrnnn Ppak
2.35
1 3C -P F O A
417.0/372.0 Da Baseline Missed
n Wrnnn Peak___
PFOS 1 499.0/80.0 Da a Baseline Missed n Wrnnn Ppak
1
PFUnA 1 563.0/519.0 Da Baseline Missed ___ h Wrnnn Peak___
13C-PFOS 503.0/80.0 Da Baseline Missed n Wrnnn Ppak
13C-PFUnA 565.0/270.0 Da a Baseline Missed n Wrnnn Peak
PFC O.IOppb
2.31
PFNA 1 463.0/419.0 Da Baseline d Missed n Wrnnn Peak
i N
PFDA 1 513.0/469.0 Da
Baseline d Missed
n Wrnnn Ppak 4.12
I
PFDS 1 599.0/80.0 Da Baseline Missed ___ n Wrnnn Peak___
13C-PFNA 468.0/423.0 Da Baseline Missed
Wrnnn Peak
13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Peak
13C-PFO S
503.0/80.0 Da q Baseline Missed
n Wrnnn Peak
PFDoA 1 613.0/569.0 Da Baseline Missed n Wrnnn Ppak
13C-PFDOA 615.0/169.0 Da o Baseline Missed n Wrnnn Peak
PFTrA 1 663.0/619.0 Da Baseline Missed n Wrnnn Ppak
13C-PFDOA 615.0/169.0 Da Baseline Missed ___ r Wrnnn Peak___
PFTeA 1 713.0/669.0 Da d Baseline Missed n Wrnnn Peak
13C-PFDOA 615.0/169.0 Da Baseline Missed ___ n Wrnnn Peak___
Before After
267
Page 16 of 242
Ion Ratio Confirmation
p. 92 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
PFC 0.10ppb
Before After
268
Page 17 of 242
P. 93 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
Project Data File Acquisition Method
Ewan's ProjectsVPFCs 02081 OtACalLong.wiff PFCkinetexLong.dam
Sam ple Name A cquisition Date A cquisition Method S a m p le ID Sam ple Com m ent
PFC 0.5ppb 2/8/2010 6:30:24 PM PFCkinetexLong.dam LCMS2-85B
Results Summary
Analyte Name
Analyte RT (Expected)
PFOA 1
1.37(1.36)
PFNA 1
2.34 (2.42)
PFOS 1
2.36 (2.53)
PFDA 1
3.34 (3.34)
PFUnA 1 4.12(4.06)
PFDS 1
4.13(4.10)
PFD oA 1 4.77 (4.63)
PFTrA 1
5.29 (5.14)
PFTeA 1
5.74 (5.74)
IS Name 13C-PFOA 13C-PFNA 13C-PFOS 13C-PFDA 13C-PFUnA 13C-PFOS 13C-PFDOA 13C-PFDOA 13C-PFDOA
IS Area 2869800 2221600 6831100 2150700 185430 6831100 197790 197790 197790
Result Table Algorithm Used Instrum ent Name
02081 OSamplesLong.rdb IntelliQuan API 5000
Injection Vial Injection Volum e Sam ple Type Dilution Factor W eight to Volum e
3 5 Standard 1.00 0.00
Analyte Area 57106
112750 344960 101810 75738 378480 59384 42023 31152
Exp Cone (n g /m L )
0.500 0.500 0.500 0.500 0.500 0.500 0.500 0.500 0.500
Calc. Cone (n g /m L )
0.444
Accuracy (%)
89
0.467
93
0.488
98
0.477
95
0.411
82
0.490
98
0.518
104
0.567
113
0.587
117 /
Mod? No No No No No No No No No
269
V'
( l 1"
Page 18 of 242
Columbia Analytical
IRC Summary
Analyte Peak Name
PFOA 2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 S_13C -PFO S PFDA 2 S _ 1 3 C -P F D A PFUnA 2 S _ 1 3 C -P F U n A PFDS 2 PFDoA2 S_13C -PFDoA PFTrA 2 PFTeA2
p. 94
Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
RT (Expected)
1 .3 7 (1 .3 6 ) 1.36 (1.36) 2.32 (2.44) 2.33 (2.44) 2.35 (2.53) 2.35 (2.53) 3.36 (3.34) 3.33 (3.34) 4 .1 3 (4 .0 6 ) 4 .1 3 (4 .0 6 ) 4 .1 3 (4 .1 0 ) 4.76 (4.64) 4.76 (4.64) 5.30 (5.13) 5.74 (5.56)
Area
148080 2869800
32633 2221600 110050 6831100
15344 2150700
18267 185430 117570 19133 197790 12533 12051
Modified?
NO No No No No No No No No No No No No No No
C alcu la te d Ion Ratio (Expected Value)
2 .5 9 5 0 .2 5 0 .2 9 1 9 .7 0 0 .3 2 1 9 .8 0 0 .1 5 2 1 .1 2 0 .2 4 2 .4 5 0.31 0 .3 2 3 .3 3 0 .3 0 0 .3 9
270
Page 19 of 242
Columbia Quantitative Peak Review
p. 95 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
PFC 0.5ppb
PFOA 1 413.0/169.0 Da c Baseline Missed r Wrnnn Peak
13C-PFOA 417.0/372.0 Da c Baseline Missed n Wrnnn Peak
PFNA 1 463.0/419.0 Da Baseline o Missed n Wrnnn Peak
13C-PFNA 468.0/423.0 Da Baseline Missed n Wrnnn Perak
PFOS 1 499.0/80.0 Da Baseline Missed n Wrnnn Peak
4.12
13C-PFOS 503.0/80.0 Da
Baseline Missed n Wrnnn Perak
4J3
PFDA 1 513.0/469.0 Da Baseline Missed n Wrnnn Peak
13C-PFDA 515.0/470.0 Da Baseline Missed n Wrnnn Perak
PFUnA 1 563.0/519.0 Da Baseline Missed ___n Wrnnn Peak___
13C-PFUnA 565.0/270.0 Da Baseline Missed ___ n Wrnnn Peak___
PFDS1 599.0/80.0 Da Baseline a Missed n Wrnnn Peak
13C-PFOS 503.0/80.0 Da Baseline Missed ___n Wrnnn Peak
PFDoA 1 613.0/569.0 Da Baseline a Missed n Wrnnn Peak
5.74
13C-PFDOA 615.0/169.0 Da c Baseline Missed n Wrnnn Ppak
PFTrA 1 663.0/619.0 Da Baseline Missed ____n Wrnnn Peak___
Ll......... .........
PFTeA 1 713.0/669.0 Da c Baseline Missed ___n Wrnnn Peak___
13C-PFDoA 615.0/169.0 Da o Baseline a Missed ___ n Wrnnn Peak___
Before After
13C-PFDOA 615.0/169.0 Da o Baseline Missed ___ n W rnnn Peak___
Page 20 of 242
271
Ion Ratio Confirmation
p. 96 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
PFC 0.5ppb
Before After
272
Page 21 of 242
"' i Columbia J A u slvtioi Services
p. 97 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
Project D ata File A cquisition Method
Ewan's Projects\PFCs 020810b\CalLong.wiff PFCkinetexLong.dam
Sam ple Name A cquisition Date A cquisition Method S am p le ID Sam ple Com m ent
PFC 1.Oppb 2/8/2010 6:37:56 PM PFCkinetexLong.dam LCMS2-85C
Results Summary
Analyte Name
Analyte RT (Expected)
PFOA 1
1.33(1.36)
IS Name 13C-PFOA
IS Area 2783400
PFNA 1
2.31 (2.42) 13C-PFNA 2203300
PFOS 1
2.34(2.53) 13C-PFOS 6650300
PFDA 1
3.32 (3.34) 13C-PFDA 2119100
PFUnA 1 4.12(4.06) 13C-PFUnA 155180
PFDS 1
4.12(4.10) 13C-PFOS 6650300
PFD oA 1 4.75 (4.63) 13C-PFD0A 234070
PFTrA 1
5.29 (5.14) 13C-PFDOA 234070
PFTeA 1
5.74 (5.74) 13C-PFDoA 234070
Result Table Algorithm Used Instrum ent Name
02081 OSamplesLong.rdb IntelliQuan API 5000
Injection Vial Injection Volum e Sam ple Type Dilution Factor W eight to Volume
4 5 Standard 1.00 0.00
Analyte Area
140010 240150 740480 222700 178520 800030 129680 86772 58420
Exp Cone (n g /m L )
1.000 1.000 1.000 1.000 1.000 1.000 1.000 1.000 1.000
Calc. Cone (n g /m L )
1.070
Accuracy (%)
107
0.975
98
1.060
106
1.020
102
1.200
120
1.040
104
0.985
99
1.020
102
0.928
93 /
Mod? No No No No No No No No No
273
Jr ^
y ^ i j l' 1U ( (0
Page 22 of 242
Columbia
IRC Summary
Analyte Peak Name PFOA 2 S_13C_PFOA PFNA2 S_13C_PFNA PFOS 2 S _13C -P F O S PFDA2 S J I3 C -P F D A PFUnA2 S_13C-PFUnA PFDS 2 PFDoA2 S_13C-PFDoA PFTrA 2 PFTeA2
p. 98 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
RT (Expected)
1.35 (1.36) 1.31 (1.36) 2.28 (2.44) 2.27 (2.44) 2.30 (2.53) 2.31 (2.53) 3.31 (3.34) 3.32(3.34) 4.13 (4.06) 4.11 (4.06) 4.12 (4.10) 4.75 (4.64) 4.75 (4.64) 5.31 (5.13) 5.75 (5.56)
Area
331790 2783400
72566 2203300 228880 6650300
29896 2119100
41151 155180 253850 42524 234070 29476 19065
Modified?
No No No No No No No No No No No No No No No
Calculated Ion Ratio (Expected Value)
2.37 19.88 0.30 9.17 0.31 8.98 0.13 9.52 0.23 0.87 0.32 0.33 1.80 0.34 0.33
274
Page 23 of 242
iiim fesa
Quantitative Peak Review
1^3^
Created with Analyst*fteporter Printed: 14/02/2010 6:41:00 PM
PFC 1.0ppb
PFOA 1 413.0/169.0 Da g Baseline c Missed r Wrnnn Peak
1
PFOS 1 499.0/80.0 Da Baseline c Missed r Wrnnn Peak
13C-PFOA 417.0/372.0 Da c Baseline Missed
Wrnnn Peak
l
13C-PFOS 503.0/80.0 Da d Baseline Missed n Wrnnn Peak
PFNA 1 463.0/419.0 Da
Baseline o Missed n Wrnnn Peak
3.32
13C-PFNA 468.0/423.0 Da a Baseline o Missed r Wrnnn Peak
J 3.32
i .-IMS
PFDA 1 513.0/469.0 Da o Baseline Missed r Wrnnn Peak
4.12
13C-PFDA 515.0/470.0 Da Baseline Missed
Wrnnn Peak
PFUnA 1 563.0/519.0 Da c Baseline Missed r Wrnnn Peak
13C-PFUnA 565.0/270.0 Da c Baseline Missed n Wrnnn Peak
PFDS 1 599.0/80.0 Da o Baseline Missed ___n Wrnnn Peak___
5.29
13C-PFOS 503.0/80.0 Da q Baseline o Missed ___n Wrnnn Peak___
PFDoA 1 613.0/569.0 Da c Baseline Missed r Wrnnn Peak
5.74
13C-PFDOA 615.0/169.0 Da Baseline Missed n Wrnnn Peak
PFTrA 1 663.0/619.0 Da Baseline a Missed ___p Wrnnn Peak___
13C-PFDoA 615.0/169.0 Da Baseline o Missed n Wrnnn Peak
PFTeA 1
713.0/669.0 Da c Baseline g Missed
r Wrnnn Peak
13C-PFDOA 615.0/169.0 Da c Baseline Missed ___ n Wrnnn Peak___
Before After
275
Page 24 of 242
Columbia
Analytical!
Ion Ratio Confirmation
1.35
p. 1 0 0 Created with Analyst Reporter Printed: 14/02/2010 6:41:00 PM
PFC 1.0ppb
PFOA2 413.0/369.0 Da c Baseline o Missed c Wrong Peak
PFNA2 463.0/219.0 Da o Baseline Missed Wrong Peak
PFOS2 499.0/99.0 Da Baseline Missed Wrong Peak
PFDA2 513.0/269.0 Da Baseline o Missed Li Wrong Peak
PFUnA* 2 563.0/269.0 Da c Baseline Missed l Wrong Peak
PFDS2 599.0/99.0 Da Baseline o Missed Wrong Peak
PFDoA2 613.0/169.0 Da Baseline Missed Wrong Peak
I'................ 5.
____________ t
PFTrA 2 663.0/169.0 Da Baseline Missed d Wrong Peak
PFTeA2 713.0/169.0 Da d Baseline Missed g Wrong Peak
Before After
276
Page 25 of 242
