Document b86ZgmNpDvM7qNnKownN233

Wildlife International, Ltd. " Project Number 434A-103A PFOS: A 96-HOUR TOXICITY TEST WITH `THE FRESHWATER ALGA (Selenastrum capricormutum) ADDENDUM `WILDLIFE INTERNATIONAL, LTD. PROJECT NUMBER: 3M LAB REQUEST NO. U2723 SerieUsSS8,50EnviErcoonlomgeinctaall fPercottcsiToenstAGguiedyelines 'OPPTS nd Number 850.5400 `OECD Guideline 201 454A-103A This study was reported using et substance purityof90.49%. The test substance ci haracterizoapttrisoienahwtaoesdswrieesvriesesdiromepnvli9ys/ec7d/ob0ea0ssatenedddatnbhyetthphuroitteyiwoeaosnftdhteetpeturimoitnypeaduntailoysbseeso.8r6.T9ah%ce.torTrehsoeufsSp0o.9n6s03o.r Noattemptwas madeto recalculate analyticalstandardconcentrations of measured concentrations fromorigainalyrawdpaatga.fAosmstuhceh,nmailnorrepeorrrtor4sdduheetomrooeutnrdeicnegnmt aeyrbiecpraesoefnatn.alAystist. acihsethde = ~=Z 389g3= Wildlife International, Ltd. -2- Project Number454A-103A REVISED STUDY SUMMARY BASED ON A PURITY OF 86.9% Soo NI Corpora SPONSOR'S REPRESENTATIVE: Ms. Susan A. Beach ERA Auch LOCATION OF STUDY, RAW "DATAAND A COPYOFTHE TTDOPFROTCRNAATBIEORN `Wildlife Intemational Ltd. `Easton,MD 21601 SSOA103A `TEST SUBSTANCE: PFOS(Perfluoroocatane Sulfonic Acid Potassium Salt) STUDY: PFOS: A96-Hour Toxicity Testwiththe Freshwater Alga (Selenastrum capricornutum) MCEoACRNTMREAATSIUORNESD:TEST NegativeControl, 5.3, 11, 20,42, 83and 172 mga.i/L `TESTDATES: Letom oFEXPOSURE: TEST GROEN sRourscesOF TEST 96Hours BeStmtan-raAiptroiln1A2p,a 1169,919999 `Experimental Termination -April 21, 1999 Fre Nor CamaraSpear Wtioldns maNtaiionodn$s16L0i1d CELLDBT = T 72-HOUR EC50: $meain, 95% CONFIDENCE LIMITS: T5mgaill 96.+0UR ECI0: 95% CONFIDENCE LIMITS: gma 4land 48 mg ai/L 9S6-HNOURFEbCe50N:ceLos: 6S8mgYai/nlgain 9956%-HOCUORNFEICD9E0N:CELIMITS: Tom NOARE: 96-soUR NOAEC: 110312am6ga1i4/7lmg aL 2mgain 2mgain Wildlife International, Ltd. oe REVISED SUMMARY (Continued) AaRmEAeUNDER THE GROWTTT 7925-%HCOOUNRFEISDOEN:CELIMITS: STumwgdaPimngail ho 9o6st-cHoOUnRrEpC1m0C:EIMTS L | Budla meaid | 9965-%HCOOUNRFEICD5E0N:CELIMITS: : I SB `mgail gain, 9956%-HCOOUNRFEICD9E0N:CELIMITS: 72HoURNoASC: S6H0URNOAEC: 112309 amngdai14l9lmgaL mail s2mgail CT2R-OHWOTURTEACTSEO: 95%CONFIDENCE LIMITS: 9956%-HOCUORNFEICD1E0:NCELIgTS: 96-HOUR EC50: 95% CONFIDENCELIMITS: 96-HOUR EC90: 95% CONFIDENCE LIMITS: 72-HOUR NOAEC: S6-HoURNoAEC: Usmgainl 9912a 7mn gaid /L S572Tamga6i0/ml ail 121 mgai/L 110 133amgn aid /L >112mgai/l Not Calculable a2mgaill 2mgail Project Number 4564-1034 3| Wildlife International, Ltd. - `CERTIFICATE OF ANALYSIS Prec Number 4544-1034 INTERIM CERTIFICATE OFANALYSIS Revision1(9/7/00) Centre Analytical Laboratories COA Reference #: 023-0184. 3M Product: PFOS, Lot 217 Referenc#e: SD-018 Purity: 86.9% . pT wr ew Tentifcaitokn To E T ee | Metals (ICP/MS) Tae 21.. CMaalgcnieusmium S o BE e ee BI Sl 51. 0g.0000l5wwAvwt% 34.. PSootdaisusmium? 5. Nickel Los a eee n chad 431463899 uwtmha%% a Ses wy 6. Iron 7._Manganes CRs Ee ie 6.0005 w/m% 7 <0000wwe Tooutall% purmputri yGE) EEEE=RBSESEPE Bawlwiiens | "Total % Impurity TITER None Detected [aco Related Compounds -- [THI Tee ee [PuriybyDSC___ organicAnions (IC) I. Chloride 2. Fluoride 3. Bromide 4. Nitrate 5. Nitrite 6. Phosphate 7._Sulfate* OrganicAcids* (IC) 1. TFA 25. PrFoPaA 4 NFPA `ElemIe.ntaClaArnbaolnysis 2. Hydrogen 34. NSuiltfraorgen 5. Fluorine [7 7 mE |27E SER Ta ian Een ne Sarin a Alben EA : TT LE Se CE] Jie a BEER i pei snl dean Wd cll 1. TheorVealtuei=1c7a.8l% 2 `TheoreticalValue =0% 43 `TThehoreetiocarlVVeaallutueei==5c0.%a95l% 3. Theoretical Value =60% NorApplies] 1 <0.01Swth % 2 059wm% 3 <0.040 wnt % 4. <0009wit % 5. <0.006 wAwt% 6. <0.007 wiwt% 7. 876 wiwt% 231. Sheen <<00.11wwtt/hwnt%% 4028 hv 1 11248 wthn% | 2. 0244 wm% 43. s17844wwt An%% | 5. 54.1 wt/m% conzsaisa Page 10r3 INTERIM CERTIFICATE OFANALYSIS Centre Analytical Laboratories COA Reference #: 023-0184 DateofLast Analysis: 08/31/00 Expiration Date: 08/31/01 Storage Conditions: Frozen 0-10C Reassessment Date: 08/31/01 "Purity = 100% - (sumofmetal impurities, 1.45% +LC/MS impurities, 8.41%+norganic Fluoride, 0.59%+NMR impurities,1.93% organic acid impurities, 0.38% POAA, 033%) Total impurity from all tests = 13.09% Purity = 100% - 13.09% = 86.9% ?Potassium is expected in this saltform and is therefore not considered an impurity. PobusreirtvyebdyfDorStChisissgaemnpelrea.lly not applicable to materials of low purity. No endotherm was "Sulfur in the sample appears to be converted to SO4 and hence detected using the inorganic anion method conditions. The anion resultagreeswell with the sulfur determination in the elemental analysis, lending confidence to this interpretation. Based on the results, the SO4 is not considered an impurity. TFA HNFFPBAA PFPA `THreipftlaufolruooarcoebtiuctyarciicdacid Nonofluoropentanoic acid Pentafluoropropanoic acid "Theoretical valuecalculations based on the empirical formula, CsF17S0K;* (MW=538) "This work was conducted under EPA Good Laboratory Practice Standards (40 CFR 160). COA023.018A Page20f3 INTERIM CERTIFICATE OF ANALYSIS Centre Analytical Laboratories COA Reference #: 023-0184 LC/MS Purity Profile: Impurity 122 -- esr iy ET Te 1 a1 CC To ma Note: The C4 and C6 vawlereucalceulasted usingthe C4 and C6 standard calibration curves, respectively. The CS value was calculated using the average response factors from the C4 and C6 standard curves. Likewise, the C7 value was calculated using the average response factors from the C6 and C8 standard curves PreparedBy. _______ _ David S_ Bell Date Scientist, Centre Analytical Laboratories Reviewed By: John Flaherty _ Date Laboratory Manager, Centre Analytical Laboratories COA3018A Page of 3 ilCOdTlOiXfICeOLIOnGYt&eArNAnLaYTtICiAoLTnESaTIlNG, SLERtVIdCE.S 7 5 Ms. Susan A. Beach 3E9n3Mv5CiosBroupnsomhrenaAttvaielonnuLaeboratory 320M. BBuoilxd3in3g332-135-09 St PaulMinnesota 55133 Dear Susan ET September27,2000 1 LEAL he g OLEEY pe A Enclosed please find Addendums for Wildlife Intemational, Ltd. Project Numbers 454A-101, 4544-102, 454A-103A, S4A-104, 4S4A-105, 454A-106, 454A-107, 454A-108and 454A-109 16y0uhaveanyquestion, please ltmeKnow. Sincesy, KExeoenpsin Kurt R. Drottar SeniorBiologist 8598 Commerce Drive-Easton, Maryland 2161 ToL 10-22-8600-Fax 410.622.0632 - Ems ecotoxawidifetermtioatcom Evga Ofc OmstemseTeAg. WAANL ari ToaNetrtins Ta OTES18 FSOSHS Er socpsaowririot