Document b5zpYpjE9Kg6m03pmM84gvyKk
ARRRG - O04
ACUTE TOXICITY TO FISH (RAINBOW TROUT)
TEST SUBSTANCE
Identity: ArefmeirxrteudretocaonstPaiFnOiSng, pFeCr-f8l5u,oroorocatsanaescuolmfponoanteen,twhoficFhC-m3a0y52a.ls(o1b-e Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: is FC-3052.
CTuhrere3ntMipnrfoodrumcattiioonn liontdincuamtebseritwisaasmnioxttunroeteodf.5T%hePFtOesSt,sa3m0p%le
dociteytlhyslulefnaeteg,ly6co%l sboutdyiluemthdeerc,yl33su.l5f8at%e,wa0t.er3,6%10so%dSiuulmtolanueryflosaumlefra,te6, %ansdodium
0.06% benzotriazole.
The following summary applies to a mixture with incompletely crehfalreacctttehreizteodxiccoitnycoefnttrhaetfilounosroofchimepmuirciatliecso.mDpaotnaemntayofntohteatcecsutrsaatmepllye.
METHOD:
Method: Environment Canada Method EPS 1/RM/9 July 1890
TGyLpPe:: NAocute static
YSepeacriceosm:plOentceodr:hy1n9c9h9us mykiss
SAnuaplpyltiiecra:l SmporniintgorCirnege:k TTreomupterHaattcuhreer,y,pHL,ewcionsdtuocwtni,viMtyT,.and dissolved
oExxypgoesnu.re period: 96-hours
SKtaartbiesrtimcealthmoedt,hwohdisl:e tLheCNOvaElCuewsacsaldceutleartemdinbeydtvhieaTFrisihmemr'esdESxpaecatrTmeastn.-
Tost fish age: Juveniles Length and weight:
Length = 38 -- 49 mm
Loading: 0.242 gL Average weight = 0.648 g (wet) .
PTreesttrceoantdmietnito:nsN:one
Dcialrubtoino.n Wwaatteerr:waDsecahelroartiendafteodr C2i4t-yhoofurDsulpurtiho,r MtoNustea.p water, post-
DilutioHnawradtneerssc:hemistr4y4:mg/L as CaCOs
Alkalinity: pH:
44 - 46 mg/L as CaCOs 7.82
--
Laingdht8inhgo:urCsoodla-rwkhwiatesfmlauionretsaciennetdltighhtrs.ouAgdhaoitluhyetphtoesttoipnegrpieordioodf.16 hours light Stock and test solution preparation: The test material was added directly
to the dilution water on a weight/volume basis.
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CStoanbcielinttyroafttihoenstedsotscihnegmriactae:l sOonlcuet.ions: Not noted. Exposure vessels: 40 L glass aquaria containing 26.7L test solution.
Number of replicates: 2
NNuummbbeerrooff cfiosnhcepnetrrraetpiloincsa:te:fiv1e0plus a blank control Water pchHemriasntgrey(d0-u9r6inhgoutrhse):study:
77..1356 --- 77.,8529 ((c1o2n5trmogl/eLxpeoxspuorseu)re)" Temperature range (0-96 hours): 14.--4 15.4 C Conduct1i2v6i--ty1r3a6npgmeh(o0s-/96omho(ucronst)r:ol exposure) Dissol1v3e6d --ox1y4g4epnmrhaonsg/ec(m0(-19265hmougr/sL):exp_osure)
45..48--99..10 mmgg//LL ((c1o2n5trmogl/eLxpeoxspuorseu)re) b* e1c2a5umsge/tLhe(ihnitgehremsetditahtreeecocnocnecnetnrtartaitoino)nsexupsoesdurreesvualltuedesinutsoetdal
mortality by 48 hours.
RESULTS Nominal concentrations: Blank control, 62.5, 125, 250, 500, and 1000 mg/L Element values: 9966--hhoouurr LNCOsoEC= 1=7172(5CImgno/tLcalculable) mg/L
Element values based on nominal concentrations
RSeubmsatraknsc:eTReesmtianrgkwsafsielcdo.nTduhcetveadlouensarmeipxotrutreed
as described apply to that
in the Test mixture and
not
the fiuorochemical proportion alone.
CONCLUSIONS
The test sample 96-hour LCso for mg/L. The 96-hour No Observed
rainbow trout was determined Effect Concentration (NOEC)
to be was
177
determined to be 125 mg/L.
`Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY
tReesltiianbgi.litHyo:weKvieirmi,stchhersatnukdinygla=c2k.s Tihnifsorsmtautdiyonmoenetpsurtihteyocfrtithereiatefsotr squuablsittaynce
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and it lacks analytical confirmation of the amount of fluorochemical proportion in solution,
REFERENCES
--------
`DTihviisstieosn,towfaDsulcuotnhd,uMctNedatbythAeSrcelquCeorsptoorfatthioen3, MEnCvoimropnamneyn,taElnvTiesrtoinnmgental Laboratory, St. Paul, MN, Lab Request number W2039-2, 1999.
OTHER
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Last changed: 6/26/00.
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yd
QR:\ 9 oT Are S =F AN
or STUDYTITLE
ACUTE TOXICITY OF FC-3052 TO
RAINBOW TROUT (Oncorhynchus mykiss)
DATA STANDARD
Environment Canada ~
Method EPS 1/RM/9 July 1990
STUDYCOMPLETED July 19, 1999
TESTING FACILITY.
AScl Corporation
Envi4r4o4n4meAnitraplarTkesBtoiunlgeDviavridsion
Duluth, MN 55811
Tel. No. (218) 722-4040
Fax No. (218) 722-2592
STUDY IDENTIFICATIONNUMBERS
AScl Study ID# 5010-068
3M Lab Request# W2039-2
J Envi"rAosnemSeundTyes1i0o1g5D0i1v0i0si6o8n
SSppoonnssoor:r StIudMy IDI W20392
1
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Sponsor'sLabReguesy foams --------] |{
| Data Standarg
|| sponsor f | Sponsor'sRepresentative
|
I
{
1.0 GENERAL INFORMATION
EnvirAoSnmeelnSttaulAdTSyees1lt0i4Cnog5m0Dp1o0rraaigvon
RpaeitneboTwoxTircoiutty o(fOnFcCo.r3h0yn5ehggs mykiss).
|
MEentvihroodnmEePnSt ICaRnMa/d9a uly 100,
|
SS3te.rMvPiEacunelvs,i,rMBounNimled5ni5tn1ag3]32,T.e3c5h.n0o9,lo8g3y9apngaScafAevteynue, |
3SSEEu-cs0ha9nn,oAl9,o3g5ByeBaaucnshdh,SAa3vfmeeEtnnyuveSi,erroynimceenstBaulilding 2.
| |I
Tel No.(651) 778-7452, 31 Paul, MN 55133,
I I
Principal Investigator
| Project Director | Testing Facility Director
Definitive Test Dates
||[| Data Validation and Report Review
Retention of Raw Data and Fig] Report
|| Location of Raw Dag. and Final Report
L
DiSveislioCno,rp4o4ration EnvironmengayTesting |
MN 55811; 4Te4lA.iNrop.ar2kB1o8u)le7v2a2r-d4,04Duluth,
Joseph Dierkes,
0,
I
|
Donald Mount,
July 15-19, 1999,
po
|
|
I
5
{
Ten
years
from
the
date
=
study 15 completed.
|
|
MDAiSNvcils5i5oC8no1,r1p4;o4r4Taet4li,oANniEornp.vair(rk2o1Bn8om)uel7ne2tv2aa-r]4dT0,e4s0Dt,uilnugth, ||
I
SSppoonnssoorrsSu3dy ID Waoze.2 2
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EnvironmentalATseestiCnogrpDoirvaitsiioonn "ASC! Study 104 5010068
2.0 OBJECTIVES)
"tTesotdcehteemrimcianle, tFhCe-9360-5h2,LCfoyrg,raLiCnsboo,watnrdou9t6(-OhncNoOrEhyCnc(hnuosombyskeirsvse)duenfdfeecrtsctaotniccenttesrtatcioonndi)tioofnst.he || This study was conducted according to Environment Canada Reference Method EPS I/RM/S
| July 1990, "Acute Lethality Test Using Rainbow Trout."
3.0 TEST METHODS
[Test Chemical 1 |{ Test Organisms
|| II |
1
| (DiPlCutWiaotneWra)ter
| Properties: FC-3052, dark amber/brown colored liquid, specific gravity | ca. 1.1 (water = 1), vapor pressure ca. 16 mm Hg calculated at 20C, stable, and miscible with water.
T1e0s0t0comngc/eLntrbaatsieodnso:n r0a(ndgiel-uftiinodninwgatteerstcroensturlotls)., T6e2s.5t,so1l2u5t,io2n5s0,pr5ep0a0readndby |
direct addition to each replicate test chamber.
| Source: Healthy, juvenile rainbow trout (Oncorhynchus mykiss) obtained
from a commercial supplier.
i
Hwaotledri.ngL/eascscltihmaanti1on%: moHretladliatnydocaccucrlrimeadtdeudrifonrg 2h4olddaiyngs/aincctlhiematteistondipleurtiioodn.
At test weight
termination, with a mean
they were weightof
3.8-4.9 cm in length and 0.414-0.928 g in 0.648 + 0.169 g. Loading rate was 0.242
gL.
|
Use in test: Did not feed 24 h before test initiation. Used 10
|
organisms/replicate and 2 replicates/test concentration.
Scohuermciec:al Daencahllysoirsiniastiend RCaitwy DoaftDaulPuatchk,agMe.N, tap water. The most recent |
CChaarCaOci)eraisntdicas:pHAtofte7s.t82t.imeA,ertahteedwaftoerr
mhoardeathhaanrd2n4essh
of 44 prior
mg/L (as to using in
the
||
test
SSppoonnssoorr:SuIdMy IDF W2039:2
3
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EnvirAoSnelmeSntauAdTSyeesl(iDCn4ogrSpbootiroast0oigonsn
|| CEhxpaomsbuerres
| I
|
l |I Observations
|
|f Endpoint | Catcutations
L
Type: 40-L glass aquaria.
I
StUeosslteu,tiiknoenptstestwc:oevre2er6e.nd7o,tLeaxeocrfeatpdetidluwdthiueornninwegaxttpeheerrioemxepnottessautlresosob.lsuetrivoant/icohnasmbweerr.e
During
made.
the
{
Durasion: 96 h.
|
Dfaluiolryepscheonttoplearmiposd.: 16 h light and 8 h dark, maintained using cool-white
Temperature: 15+ 1C.
:
sBtiroelsosg,icaanld: deDaadilyte-stToersgtanoirsgmasn.isms with abnormal behavior and signs of l
ddWaeaittleeyr-rmitcnehametpmieiorsntasrtyuw:reer,(e1D)mOaA,dteatneasdctcpioHnri,dtiiaantngidotnso-peAtocPtiafHlicAhacromdnendteuhcsotsdivasint(dy1.9a9lA8kl)all.inity, (2)
l |
Tn24to-i,omb4ms8ee-rd,vaS7b2pl-e,eareafmnfadenc9-t6Kc-aohrnbcleeenrttharMlaettcihoononcde,(nNtLrOaECtCi,)on.dset(LeLrCCmsgissn)ed,deatvineardmEi9n6Pe-Adh
vLiCayg ICp
and
|
Method, and NOEC determined via Fisher's Exact Test.
4.0 RESULTS
|
Test 95%
CchoenmfiidceanlceToixnitceirvtayls()P.arePnetrhteitniecntvadlauteasaarree
|
240k
LCgg:
330 mg/L (301 mg/L362 mg/L).
|
{ in Table 1.
48:h LCs: 177 mg/L (Not Reliable).
|
72h LCs: 177 mg/L (Not Reliable).
I
96-h LCs: 177 mg/L (Not Reliable)
|
|
Re9l6ihablLeC.);
138 mg/L, Confidence Interval not
| |
96-h NOEC: 125 mg/L.
I
SSppoonnssoorrs Si3nMd.y IDF W2035.2
4
-
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EnvirAoSnCmleSnutuAdTSyeesl1t0iC1nogr0pD1oi0rv.aa0ii6oo8nn
| Ranges of Water Chemistry Parameters
I |
_
_
Total hardness (as CaCOy mg/L): 44.
l
Alkalinity (as CaCOy mg/L): 44-46.
Temperaure (C): 14.4-15.4.
DO (mg/L): 4.49.1.
PH: 7157.82.
| Specific conductivity (umhos/cm): 126-185. ||
5.0 TEST QA/QC
| Mthoarntal1i0t%y.in control exposure must not be more | 0% mortality.
1|
[||
The lowest termination
DO concentration at test must be at least 60% of
the
air
4`T4he%olfotwehset DaiOr scaotnucreantitornatviaolnuemeaatsu1r5.e0dCw.as |
| satwration value at test temperature.
The lowest DO concentration observed was at
tNhOeE9C6. hoTuhretelsotwienstterDvaOl actontcheent1r2a5timogn/L I
|I
tohbese2r5v0edmagt/tLhetetstimceonocfenctormaptlieotnewmaosrt6al4i%tyoifn saturation. The data show dissolved oxygen
1
deprivation did not cause toxicity observed. |
| Test duration must be 96 h.
`The test duration was 96 h.
Reference Substance Toxicity Test [Loptionah).
(this test is| Data package for the sodium chloride test with this fish batch isinRawData Package
SSppoonnssoorr: SiIudMy IDY W2039.2
s
:
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EnvirAoSnEmeSnutdATySee1sl0iCn4ogr3p0Bo1r0r-a0io0o5n
6.0 REFERENCES
|| WAamteerricaanndPWuabsltiecwHaetaelrt.hAAPssHoAc,iatWiaosnhi(nAgPtHoAn),.D.1C99.8. Standard Methods for the Examination or]i | PErnovtierctoinomne.ntOuCaawnaa,da.Ont1a9r9i0o.. ARceuptoertLeEtPhaSli1t/yRTMe/sSt.Using Rainbow Trout, Conservation and
|| f1Hoa1rmDiE:lstto7in1m,a4t.iM7n1.g9A;.M,eCdoRri.raCen.ctLiReoutnshsao1l,2C4aon)nc:den4Rt1.r7Va.t(i1To9hn7us8r)si.tnoTno,xi1c9i7t7y. BiTorasismamyse.d SEpnevairromna.n-SKcai.rbTeerchMneot]h.od ||
|
|
TUo.xSi.ciEtny:viTrhonemeInnhtiablitPiroonteCcotnicoenntArgaetnicoyn.(I1C9p9)3.ApAprLoianceha.r
Interpolation
Method
for
Sublethal
|| West inc, &
[version 3.5.
University
of Wyoming,
Laramie,
Wyoming.
1996,
Toxstat
Software
Package,
|
|
J
SSppoonnssoorr:Su3dmy 04 2039.2
6
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CrimTAB n AScl Corporation
TABLE 1. ACUTE TOXICITY OF FC-3052 TO RAINBOW TROUT (0. mykiss)
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EFFECT AND NO-EFFECT CONCENTRATIONS WITH `CORRESPONDING 95% CONFIDENCEINTERVALS
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