Document b5w6bXb5L8bavOXgVdL1kGe70

TRADE SECRET DuPont-8861 AR226-3212 Study Title H-25134: Static, Acute, 96-Hour Screening Test to Oncorhynchus mykiss Laboratory Project ID: DuPont-8861 Author: Laurie D. Bouchelle Study Completed on : December 21,2001 Performing Laboratory: E.I. du Pont de Nemours and Company Haskell Laboratory for Health and Environmental Sciences Elkton Road, P.O. Box 50 Newark, Delaware 19714-0050 1Company Sanitized. Does not contain TSCA CB1 H-25134: Static, Acute, 96-Hour Screening Test to Oncorhynchus mykiss DuPont-8861 C E R T IF IC A T IO N We, the undersigned, declare that this report provides an accurate evaluation o f data obtained from this study. Work Completed by: 0 Laurie D. Bouchelle A ssociate Scientist HI JUIWUWTUJWoyJU D ate Issued by Study Director: A lan Samel, M.S. Research Ecotoxicologist Z l v 2*wl D ate ?- . Cut*;: f t; A C ' T ' * H-25134: Static, Acute, 96-Hour Screening Test to Oncorhynchus mykiss STUDY INFORM ATION DuPont-8861 Haskell Number: 25134 Sponsor: E.I. du Pont de Nemours and Company Wilmington, Delaware 19898 U.S.A. --'Life frutiated/Completed : December 10, 2001 / December 14,2001 -3Com p.ny Sanitized. Does not contain T S C A C B I H-25134: Static, Acute, 96-Hour .Screening Test to Oncorhynchus mykiss DuPont-8861 SUMMARY The acute toxicity o f H-25134 to the rainbow trout, Oncorhynchus mykiss was determined in an unaerated, 96-hour, static test. The study was conducted with 4 concentrations o f H-25134 and a dilution water control at a mean temperature o f 11,3C (range o f 11.2-11.3C). One test chamber was used per test concentration with 10 test organisms in each chamber. Based on visual observations, the water control was clear and colorless, and the 1.0, 10, 100 and 1000 mg/L test concentrations were clear and colorless with undissolved test material on the water surface and on the bottom o f the test chambers at test start. All water quality parameters were within acceptable limits during the Exposure o f H-25134 to the water control and nominal H-25134 concentrations o f 1, 10 100 and 1000 mg/L resulted m 0, 0, 0, 0, and 0% mortality, respectively, at the end o f 96 hours. No mortality or sublethal effects were seen in the water control test organisms. The highest nominal concentration causing no mortality at test end was 1000 mg/L. The results are summarized as follows: Nominal concentrations o f H-25134. me/L dilution water control, 1, 10, 100. and 1000 96-hour LCjo for H -25134, based on > 1000 nominal concentrations, me/L H-25134 exhibited low concern for aquatic hazard3in an unaerated, 96-hour, static, acute test usmg the rambow trout, Oncorhynchus mykiss. W h e fc , J Clements, R,, Morcock, R,, and Rabert, W. (1993). Assessing ecological hazard under TSCAmethods and evaluation of data. Environmental Toxicology and Risk Assessment. ASTMSTP 1179 PWkd ^ h . ' J S' HUgheS' 3nd M A' LeWiS' EdS )' PP 22' 39' Amencan Society for Testing " d Materials -4 Company Sanitized. Does not contain TSCA Ci H-25134: Static, Acute, 96-Hour Screening Test to Oncorhynchus myiciss DuPont-8861 TABLE 1 DISSOLVED OXYGEN AND pH OF TEST AND CONTROL SOLUTION SAMPLES Nominal Test Initiation (0 hours) H-25134 Dissolved Concentration Oxygen (mg/L) _ (mg/L) PH Water Control 1.0 10 100 1000 10.6 10.8 10.8 11.0 11.1 7.4 7.5 7.5 7.5 7.6 Test Completion 196 hours'! Dissolved Oxygen (mg/L) pH 8.7 7.3 9.1 7.3 8.7 7.3 8.8 7.3 9.0 7.3 TABLE 2 MORTALITY OF Oncorhynchus mykiss, IN AN UNAERATED, STATIC, ACUTE 96-HOUR TEST WITH H-25134 ' Nominal H-25134 Concentration ________ (mgfc)________ W ater Control 1.0 10 100 1000 Number o f Dead Test Organisms / Number o f Test Organisms at Studv Start 24 Hours 48 Hours 72 Hours 96 Hours 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 -5Company Sanitized. Does not contain TSCA CE