Document b5b1x1GVbJja2d4KXJbLGjYy3
MICROBIAL INHIBITION (DEHYDROGENASE TES -SUBSTA ICE
ACTIVITY)
Identity:A mixturecontainingperfluorooctanesulfonawthei,ch may alsobe referredtoas PFOS, FC-95, oras a component ofFC-206A. (1Octanesulfoniaccid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber was cc 785-22. The testsample isFC-206A. Currentinformationindicateistisa mixtureof0.92% PFOS, 18.75% diethyleneglycolbutylether,75.44% water,1.83% Hydroxy foamer, 1.75% sodium octylsulfate1,.2% poloxyethylenemonooctylphenylether, 0.06% sodium laurylsulfatea,nd 0.05% benzotriazole..
The followingsummary applies to a mixture with incompletely characterizedconcentrations of impudiies. Data may not accurately retlectthe toxicityof the fluoruchemical component of the testsample.
MET IOD:
Method: TTC. GLP: No Year completed: 1979
Analyticalmonitoring:Amount offormazanformed (asdeterminedwitha spectrophotometermeasuring % transmittance). Statisticamlethods: Graphed the measured % transmittance. Test organism source: Activatedsludge mixed liquorfrom theMetropolitan Wastewater TreatmentPlant(Pig'sEye Plant),St.Paul,MN. Test condition:
Dilutionwater: Deionizedwater
Syntheticsewage: Nutrientbroth(0.1g/100 mL mixed liquora)dded approximately1-hourpriortostartoftest.The mixed liquorwas kept aerated priorto the test.
Stock and testsolutionpreparation: A primarystocksolutionwas prepared by dissolving22 g oftestmaterialto 100 mL withdeionized water. The stocksolutiownas then diluted(1mL ofstocksolutionto 100 mL deionizedwater)to prepareDilutio#n1. Dilutio#n2 was preparedby adding 1 mL of Dilutio#n1 to 100 mL deionizedwater. These solutionswere used topreparethetestconcentrations. Test vessels: Not given. Number of concentrations: fiveplus2 blankcontrols Incubation Temperature: 37:t 1 'C
Totalsuspended solidson day of testing:10.6gA-(TSS). Element Basis: Microbialdehydrogenase activitbyy formationof formazan. Incubation time: 15-minutes.
RESULTS
Nominal concentrations: Blank control,10, 100, 1,000,10,000,and 100,000 mg/L.
Element values: Not given.
CONCLUSIONS
No conclusionsgiven. No triphenylformaza(nTF)standardcurvewas prepared inordertodetermine pmoles ofTF produced inthetestsolutions, thereforea, plotofpercentinhibitiovns.logconcentrationswas not constructedtodeterminethe EC50 value based on dehydrogenase activity..
Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133
DATA QUALITY
ReliabilityK:limischranking3. Study lacksdescriptioannd recordsofthe method followed.The inoculum isnot properlycharacterized.There isno characterizabonofthetestsubstance purityand the studylacksanalytical confirmationoftheamount offluorochemicalproportioninthe solutions.
REFERENCES
The studieswere conducted by the3M Company, EnvironmentalLaboratory, Lab Request Number 4970S, 1979.
OTHER
Last changed: 6/26/00
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