Document b5OQpNGgX8d7X42G9jjmQqKro

.mU/ J, I'V * '. - m. Mk i Item tl* sidrsdys cf eninais sns.ll enough in mti to b* coBvwiiwi for tup* z I sent .si n 1 ;>t. J af lor . Clw.irly, tM would It *n mportant cons i dc rat i en in xic* of n, tlcJpati-d lnirst ijnrlo.is rtni ring dietary control, adii n i * t ra t inn of precis* quantities of various heavy fevtals, coIJection of itjrcrewent, etc. To thi* er.d, we first attempted to astertsln if it would be possible to isolate a protein having the characteristics cf ectsljctiiloacln tre* rabbit Ucnt;s. SloiU'^enites of i.lU'uys :* t aim-d ire* adjlt rabbits were c.l- onatogr cn cclnnnj. oi Srphri** G-'J (fine! in 0.C2 M TiifO.OOj Cl > -, ffer. ri * t-. fractionation vii followed b) the ati'.rr'cncj at l o th 2 >' w.}' 0 210 1 iothioneiu con> t, ; .. n J ^V^.ofh, riuntij-. ' y no jroeit, > a* v*a roi' jteofr' .nine* a . 5r-' 1 2*e J urns Pat 2 sf- ? i nn.l t'>- 'Jiorhir rfMrst gl C4 h (t u ; i i v .* tlnUfP* V i-;' V.-. Hi of at-.Ji -.. .' i.rrjit inrj >| r. t'..scop v. The result' arc rhovn i.. ft jure J. A sloull-r cl 9 n-.. or-.l:tg ? rial it evident in the trstling e Jg*' of t. j a)'r pfiitwi peak and tf ;rvmn with a sharp cn.lsiu* pras. Ir i pattern on ..'phiiCrr rh a t o k r^p r f 1* precisely ** *nolc *, intirlpateu f*.* a S3>all ftvle.ular velc'.f protein ucn as n X * l ] othi , r l *>,4 r&lnrlJos w t? * ** l !,"vr ''rhsvtor of this ' | T t " lf Vh`: ` * fJ ! * ** 'J (.'*>* * ' J * 1 * >4 a-J VJ'Jftf** lor l'i * rt ; u r i f t s , * i , j i fo<; t f. > .t i f t r t of. t, i V.t p f o r * * ri *-' fu' f>*sai .ii;1"*' > *. >**. ** * * 1 *1- i ^ :*- '**.'hr'**V'v`tK * j w t o mu i a.; fwppaiJLagBwiM4 unm. *. -mm ^v^eu imi 'rui -V-^m'w-p i wyjuwu.gmi^/Lii i ..'' Ifv5?'rta.V vJ' '- --r -j s - *- . .'(''.i1" j *'. ^ t 9 F^r ' .v*^ v' y- - irl ijV'^t- .I* ?^-- ***-* mii^i r/itrfiiirtiriivi) i r iHrnif Snr^i'iiji^'Trirr- - ~r '"--* -4*1...... rV . f------- i~.-I'.'A ' IV IV J) v l *1. . jI.ivl-.DT rn t,. I M f t *. -V . ii' '** fl., {>': ., -if /'.Of M rr-.s-.'": V r-tof.i1!..' i" r. f 1 : f U 1 ;{.! . v.-n: : : . . . I*. . --at-T .>! Ml LJ w'-' :. : >' '.Hi f . ... .. <- i - i ; - i i..i r1' * ;.'-f ri **>*.* if *.t ;`<t bf t ! * I : 4 r i < 1. '.ill- r.t*f H <> J f i'. ' ">:; f f rt >) r ! ( ' .* ! ! at re i'li! t' r: 11 \ (tiff!' *r. vivo. * * ' .!: f 1 < : * /. I : i ,,v - * T" ( ' r< ; . , * ` r .'- ^ raqa<uP wigpmu'iiuy.j imj ^ f r::- g^-jj-'Ai/aw-- v-.S 0 3 ,r i ' '` ! ' ' ' - ' wM le the liver tissue* is currently ng preserved for isolation of RNA in tn effort to erploro the possibility of lnteracticn of lead with KliA in_ v 1 v u (v 1 de lj>fra) The results of the isolation of tallothionein fro* kidneys of control unisals correspond to these shown in figures 1 and Z, ccaf l r.-.irg the results of cur preliminary c *pe rittents. The cadnluit ant!' nine contents of the two principal peaks resolved l>y I i.A chrot.-, t r gr.tphy(Figure ?) are shown in T s'nfe l. The clin.-' att-gtaphir results ate characteristic of oe t! l-.-t h i one in r< ether species ut serve d in "ur i *'*o ra to if r , hi,t the 1 :si tr i ir *i.i.; .{ ferisl j y .u I ildc after completion of" t \ -i.it -.n'-r-t re t * l mptlt <?t ( f loti t. s ch ui-ief : ri .... }*t- : rad* inter} t " .i raVbs t* were ' - , 7' - r-i ` a of * - t-yh .*,;'e t ar .! f>;: t iv? ". a 1 .. . >;''*/ a re >vn i n * t/ure* ! and < r* t i v 1 t*~ rr c * 1 e.'*-, f r p iff listed ir* : i: j ?j s ; ii. ! ! . direct Siw c 1 > i r. sit' t' ..... . . ; , . , - . nr. ;';* , !i-J sr-' . . r <, f < .. f J .-. j * > f " - , ' ! > - */ eWL'Srpy; i 4$ gCft twee MMWW '3.3'3v1C'-.'V-A;7" .y3t3i*;-'v._7^: life# dSc, Ihc distinct .ujoc'at ton of H^^fi *tS orf- -iti^j ;\.i f or i a i , caditiun, and loJ strongly MiggeleJ{Flg'jre JJ tbit lead, 11 ie CAtliatiua, ii bound to mo tal iolh ion< m in rabbit kidneys. The results free. MiAF. chromatography art* in accord with this cone 1 us Ionfigure- il since the Rajor quantity of lead-cor,taming natcrial is distributed in the fractions through vhlch the two betel lotMonein peaks eserge fioe the column. Unfortunately, the rather large U concentration, in this area, coup lei! with the t.u'l .trounte . of snrml a*a iiMi- at thii stage of t' - isolation procedure, v tt . iu-.'O"! ccartt q<..r.U uMsc !.-te m;-ij * : . n*- if t'.e r tal c<r.t*nt :.d, '.e-ic, the iriultl ir. Yjiiie !l cannot bo ;m ticehul t, he (mil. lh', it n nut j.oviiMo t - -t M i *h, a i i, "cd, thv f r, ut r :: oct or. the bet at cciitent oi uii. th ic. 'in w 1 T t i> tc.iltJ'iir i r> the rabbit, 1argely j jo to t -e ** i 1 mis of :kc *;.in/s yielding insufficient aa&unta cf setterm! fer character teat inn. Thera' con he little doubt, however, of the a-. -.o< at inn of is c1 with pclallcth. loiicln in this -aalerUt, and ri. si;.f rtisst . r j c 11' t hn t the rahttt Will l-a a highly Suitable iif*iHff.l > 111., ICS cf Ui >; J wC ear , t by . * t * i ' Mrt ; t * *; *t Ur at l-e ? ,* %**'$[ -V ^. .AVj.ac, c>;-n '>"%, a j i .V f f r> ; ? ' I fi . t y * yin'M ?**$*# -*4 In order to obtain the desired quantitative estinatet of the interrelationship of the various heavy net*is vlth Metallo* thioneio in state* of lead intoxication, as well as to havo suitable quantities of tutorial available for anino acid analysis, peptide aappinf, and further rharactarizarion of lead setallothlonein, we have recently cenlod out a.chronic poisoning ecperlacnt in a larger aniaal-- a posy. Vc were able to purchase thin aninal approx eately fivo uon'-hs ago, and in tho lnterrming period, with t e aid of thr MarvarJ Veterinary Service, h*'-e a da ir.'ls cored' 'N to the nil sltn'.y increasing quantities cf ltad acetate, by :hi crt. route, u, to a final total dose of 1J prsrs daily, intreronoul cr intrsausculat adninistration was precluded owing to the rc-id/ Jw.-wtlon of threnherc* LaJ abjeest <*5 . i!ence. Initial'/, this tutorial was given by stcaach tube. It was vtrbseq.ently found that, if thlrity, the pony would drlnV the *iter>al nlied with a quart of ordinary water, thr taste apparently ot 'ring loo rnple-oar t. Sincr no specific information on load ,* miration ir. thl * specie* v*s available, the v-ocet had to he . /.jjed s rtp i t i (.* 1 ly. h>,i]* the absorption of fro* the. itest iisl tract of this ir.t is pntmbly quite snail, ^he 'arje ',oe > $ni trr d vw'tially enabled tj?riticr* in* nxira'lne to ir t!iif-o^. c'a had ^ac.-lient *uci'- with our tc polar" the ni*l aluwly, and induce tut, i>, tut at u i* to render hie critically tit. F s h. "t ;; * i\ C&cii l&vfcua* I I uwa>yweavWWjaein>tHiaa4tjey-111 Wn-e--OBMBt!" i 1^'tk'***'' ' 'ri ',( i 'i - - vm - ' * ' . . ,;. 'I*''' .-^K^-'e.-* ... -; ;)t ". . 1&aSsAnrjiiJf3W,i,. S'5 itffjWL'< wmm * i. V -'. '; "'-4 ,?c`vunlnj: <* ** i#int toreJ t*/ :> of bimonthly *"= 4%:jos*-r.ts ;.{ th< ivi'j CDiiwC > t r ( ton m . icle riovit vpr* Bd".f, ?mien' in tit? rsuga t a ;yzvxut* t# ly SO to 00 yjjn-i. -ml ticaatoi r i i , viitili til ir.it * ty fli tu ne,ul> \J? V..? r filial vaJ Our Ini tr* final i>n in f !hr t. t it.yr. I. tls* i* 1 .! ., '.:.arf;. .in.', t ii !. t'. :r. t uti^M iut*. aiti'Ci.;!* partlyslt, ten- . ; i;.. ./, or . i r > of , '.r ` n :r. *- t* u>: t <ifi r nt tioO *r i -l.iir I vy i. ?.> ;t- :-.j i . v it r i f : - ; .i: July '1, <>,, it , i'? ' j . 1 ^ ; : l i** > -i; .1 * : s i:? i'** * u . - f J it c ' ,r.,; * * r *. i r >>' ire bMr.* ; feM*rv?--' f-3f fuiorc v r- *{r. (,r .i.), n, ; {.Sr 't [ ffv * C.i Tc ffT -n- t d 1 1 > '. ' ? C.':'- ' 1 .'i tt > ti,!>;:. *r-U tM enable u* t<? u>t4j*, |w#.-.ts. .>. ? >'-; 11 i ,'r. ii'Hi.; i%ln; ihrir 1 * r j C t l ct. * to*,I * ; ; trl;1 ., ' i .-.. '*-* it,- ! I . t > of BtVf "t i t.\' '. *!} > n. { n'.if - > * t `. .* i , r i t!.o r "j-j y< ,i f *; s. iv ; t? : nur ' * * tr ; '< * itM.'.'.f i t ,-r-T1 ' 1 V.'_ nji ;t f ton' i * ifr 7 5'.'*Ct t'v p f >' - : r h;t *<-* < j i , rtM v-> -.i. tSii' C it'ju/ n m*i'. > t: ;' .. ; ( v- , ,1 : 1 it .' : . ; ; j * - : ~ -'**?* - ':v ? fV i*. c . . ; *, i , -! I *> v ` it ` ' -BS! !.-v v. S.5f -VV'. J r '-t.ctjjer cr not lead aught replace the natite ertalcf certain . txjj&lieeniytaej known to ft present tn liver tissue* o. tl - , )iv i alcohol dehydrogenase, a tine containing ctity*>e. Prel i-iar,- cvoWcc suggest'. that it cay he possible to carry our inch a !i*;> 1 trerent of rloc by lead iri vi tro arid the availability of tniiailr ^usr.t 1 tl e* of tissue f roi the leal poisoned am pal per** u? ui to search for the tji y^vo counterpart. frosts? c i Vi.-.'or. to contain verv ht fch e* nt ent r-i' .if 111: c although the jrecie foar: tonal role rf the eetal in . a i jin Is a -cert . i.. 7 lost at 1C tl-tsw alto has a high iffl 'it t i .a '>> v..; i- 3. site of lr'ury in cs itu:* l"t5io' . ;o i kt were able to secure the prostate fr- f.c ivii ; , t: v >\ . -i a:.(l fie o.. ..v t : l;i <>t 'e.id in t .:r !; will V.- sr rtai nc>l and the possible di p) n'wnt of nun'll n : xs ic\. Much, of the analytical vorL of this natj'c to be nnicrtalm during this neat year will benefit froi* the availability of i-ji table spec t rngrapb t c nethods developed In this laboratory jtm t ? t .,,; the % i 11 ancous de t rainat i on ir. biologic adterials < f .( r.ueber <>f rla.'jfits .jUSii t i t a t l ve l y . It is- proposed to .. - s.,t ;:. r t h>; r st-.Jt'-s > ;> tiii-. ;prc!?a if thi* pilot pc r 1 s. r . s ; * . If It |; ' ir I r .1 - '> 1 141 11 T i 6 1 I e^t: f t,rv>?}vs,'-ssvcsvA * ;*'*.*.. yf-!- '.'if--- 4i jagtBsjMjfi a . -/- 'fth^rher';>r nrt rijht rep)tre th* native wtt*\ of ccrtnin fs-tvll.ctr. tfhfj known to he ptre<n-?;t m Jiver tiiM.t, ?.*., liver <$ alcohol ibjhyciri'st-naso, line containing cnxy^c. Ptclja-iftaty rvi ! r,cf! suggest that it n*y be poslMe Jo cut ry not such n i 11 v 1 ..c***n of line b/ lezd in fit ro fin J the ar a i}v.h 11t y of Mi-.t ii ir ii.f!tit:e of tljioc fren tin? lrvi noisonr.! jnjal fritits ns. to search lot tbc in rjlvo r>*rt. I'l.V'ti* ic t :>'t i> V contain very high con .< (.' r i . i' '.'I' | rfco c f ,:'n J i or. . 1 role of the so, ..... : ; v i-1 : i: n . ( icsutu t i >ur xiso !,.* % Hist' j j Ii * t f -. ,. . .- JT, : , a ,.itf }`. l.;ui. in C. !*;.> ) ' t i C o ` - m. : n -* \ [, , : *. c Kric <*U'r to si iu; th? ft Off J'f icsJ 1 ,i . ; . ' , ! l ' * t'':` Ci! '..)!iv f; i ! C w t! 1 ' * . . -till !< act rtsi-'.c^ r.r.i the jeulhic <Jin~!#frptit of n"r*-'l fin Much of the (nxlytlcal soil o toi> nature to be i.i i-r t > c h tlurins tM next year will brneftt fton th? svii laM i l ty of jult&i-ie sped mjrraphlt. ex-tKoJv Jv* 1 opeil in tM' laboratory frr.tJi.:jf tfco *i sc; It *,-.< o \j > .! . t ? mina t i on Ir. biologic a#trlls of i r-ur'iif 0? nuaatitativr ly. It t pfot-ovri to :s Jitiuc t- further stiititr* In tM* species if I'.li pilot <> ; ^ 11 VI' S', Vi !m4J t'j !i".| C I flufll trfj-.l i a' 11 V>!'rJ, V . ft- t t I > . r nth .j.ii *'i~ I'nSine "f PV. 4 --'*S`V i;a .C-'v, fi-ia'-ffsyi&t.n F'fJ-c / *< h<r in)itt>1 previously that the facultative na.sr:>e. r-iV.-W ?cvir.v-;<.j Vit.yrSZ ei~ `7 >*' * 4mm!ki*!W JWJPJ ID1! WWI1.U JL ^.'iiinmijpaP"<wggww ^BMS9f^P|R i';. K: ' T ?v- & r-.'"- ju'r-v** attractive b * * potto I. 3 <vrM4 far ptm!l* rf ht-avy s.Pt*l Bv'tabaiiiV n.ut Jnfo*lc*rlmi. Our'iBit progt-eM report itvtcts ptt*lt*J`!lc!S rujilavjriit {, :? i <1 itTetpt* to siM' i:';1 a -tsVoitC sift to ivi 5r.t xutict d*i out furvtj o: tl-u ir.u;cf !r j.i ;-r-'cht-s (,.*r r-rov u r iKr.j. SSiti.i,. .ft; f,( v-.ii ..'.vs !< <?!'. s: '' >o c i; i T" : . . . 4. < -,U : *. : ( .* I- : tn i ; * : (. . . ; S' l>. c , - ' " . * ' i. L. V . z - - *. i ' rf >. t. -.; i. v t . i ;... t : i. %' ; ' ` ; f r . v ; * ; > r- .* (. yrci > ? i t \ '\\*r :i * , t 1 * ' : I r : -* *' j'tf * *.* . . * . '> - -; r-v \ c r * 1 * * i a*, , * , * 4?' `wtrif1' ; .* * ' \h * iv*i * ; IN* s ; * !>*',. I -* ; * ** * * : * ** * * . !t " ; ,u -1 * * * . f ' i * ; + * . ;*p* i ^ : * ; 1 k * r ' r r. ^ - ' f V' hr. H t m v '.j - -V r-' . 'r/?v`v- 'I S*'ij t!t i it il-l , MJ-Gttttlfc Ui4( .>~ 50* V?n:-K '#< r*I grusi o f ^itr, > 'M it .' fr-joi rrd, u ^ r r j er i i.i ir. I liter 5r5esu.ii, r flmli, vl.ilr fir . zttnnr.'i ; r^iis J-.r n<t *.iw nutntlos'iii rj-*riu. ?? it *e,i f-vi.td to t-f tfitwncRt t'i fr >'!. .luf=)iC*U> i*l tur* v i t'i'* &X mnisEs ' u ;> r s tr*-. t.%; !, , K ` : t: t - 1 \r. ;r.*i *vi;i 5 :vj frc^.- ;/kt sliuVt-** r \iy> of the Situ sveie (. I. rc tro>4: ;c i<u? * ' . j r: w * v ii ri'.u tuiini f y c^' A^ .5 rr*-/;. * of t:.r* . r. J f , is !>o* t V 4> J *. .-t ., ? :. f'C'er v ti . t lr ST fii- It. rM-.il ly k**t5tf lilt* J**!-/ inrroii j n utw* It, ptgiieit : f ..: -1 - : ii.l it.:-;:.: n i`i J.fiuc i .`ru-r thv * ic. -.1 ! ! u* < ; i. .*- it-* Ug {*?>** ..si., .i .* * l- , .* >i i.t'.r Ji: it. tlj* *. te t ri,oi.u s n*, ;*t r*|>i t n>- tt. {.ft*** 5 ft inn e.` r / d li t * 4 l S .: * ./ f-Hii si 45 U ' . 5' . it t .. T-iK-rc .' iJes- J.; ' f hi ; 1 t'i t j - . i % ,i; U:. fi ' f :* l - t o ! r . * i >:- .* . .< . i- i, t- Is - : t* ..! *. *: * rg --! iiv t ; / * , -* f ; , r' *' , i. r r * ( ; .! ,* '*> - J . r ` ' . ' i r . * i - * . * i1 : - - t - .. f. * -: - . tr w * % !i ViO*v . ' "*.'"* V. . >' - v ,.%*&, ^...'.sv*. >v. -*.* . -f-;'' ;x p." i" t. j; ' - h.' HtJ.ili' t !i>n vj V , 1I , i r,h j > i t i { is * nv*l p rc *' -i v > i >*.Iy -y-.i i * :o viiiti.'.iO':., Kifc*pv tonctrrrtion. f i n-t li'v'i i t < * .t'. -j ' C s.t !, i.'Ii!i>n. ; tr ' 1 y-.I l> .'/r r .v' t`>* i*.V.bi t '.* i// '< i.. 'ir f,f I * s* v i o' i ft;; .. -r.\* t - ; : i:,i . i: ti`T.;S jj r. '* v<jTf : #t > t ? fi> !.y ' >. Ji. At ^ t <, rt> j : 5 si >is a Is c ti; f ijiifti i rtj.U 3. Thr,-, - i. . j i r. v.-V : -. ',! it* ?i } c r:>v. l<;Cw 1! af ; i-i. !' s'-ir*' .' ft'r. ..i jt j : c'. v r. r >:: ' r. > ' ^'1^;'` t'' 1 f 'ft b* : !; (*; > . -v \ - t . . n ! f { ; r t to - v ! Ptoi . _ Hi, * V' * f V t * , ft !*.. > re, ?1 ,Vi,' , it Wtc'. ' ;. - - , ! ' ; * --*** * ' r . ...i. , :;, `~ .; .-*!!, ., - ; < r A apA . * . , . .. ( . t : - - ; -V- I-?-!-- ! ' a * ` t ] ` > '* *<.*> r. ry t t< ' . . : iT> ' I - t - > T I 'rW'SL*^ v* X ^ iVX- ? s`ktjT .* r.y?>i: V t oci.ur' ja t!ic prfi-si'f-i vi I - 0% ii it?r t-ach of tbiarwino si,4: j/i*J i'r l tn' g! hjctfn. "nJrr the <n<5l tien' enployc-l f>*r * < nt r>? rj.'.'rt* ri ar-aclirte rt)r,cl refill for c f 'hrao cs hi : ; % -.*.t rcnf i r*'eJ j 1:hot.-^h vc chtrc:el I-*t rojulri far thr cpt1 - ;il rc'r.trrtrdf iw for each cf 'he Thr ^rowrh of the orysrtijst aa a function .it ir *of.ict or cor'eor. vrt t i .o.i i> tin v.; In r; T ' . vo -. . oi c<* cort r.r.f 4 ort cf 1 ' v''? ho -'pin's! -r'.f \ ? r < v-T'.csi.t rjucri i. -t ; : p0. * . ip .'n v >: I .'! o > r o:> o r * 11] i; > 1i!>! t , f fv.Ii ]N * V. ti > V *-,# * { ~ A \ or i vr- rlUV'j.T.l ft } f- l . iC ' . ^ >i rn t rc > tr t." s 9r*dl* 1V 1/* tC*? ;ki! i! -> i*jr *'/ 11 tcf. Thic - ' . * ' > -c r f - : r t \ i . j t K'l'-* ' i V ' ' ' ' 4 i " r .f . iM Vt . pov K.nsxo > k S1 :' : l - Y -5 . )* '; ? ' I* . . r - l 4 f r r* 1' rr * ; --n v ! in-j i + t: j r : ' * In .1 idiuicl to ti**t Icy<?-J lor ?hU*lre, th* .Affect of nicotinic 4Ca on the ld'i.idccc J ir.hlbiilon of >-`P* <),... r-iif.c) vis *i*e5i*cd 1% in Fi^. li and IS. No clear , relrflso! ilni* 1* oiiir.fj hetvec... the tofccent ritlooj of nscoi:..ic , ;.c;J v-.e sr.hio.tion ! Vti. U . v v b nvid-nt - f %. !ca-l ;,Hn Jne not 6c t Fy I/.: c t tinj dSt.vt!/ I y rorpe * loc ; vit>. c o s j tor. r.r -.iri^lr trci-i i si tV hv'i a a full' t . or ct i ''ilCc*;. t Ti? iCiit v* t i* .r h f; i v. I ;i t* Ol.u S f'*n . I ?.>S4V> 1 U C.^UiC ^ ,, " t rf.-. ... . t ... r 1 t!, >; t ' -. t ; >-. i>! I---J :. o c a ih - . l i Q 'r i *'. *f Si ii.tr to thc*-f lif r: k ' 4iv~, 1C - > il ;a fcU!!Xt?.i;o it f -tJ to th. !*.., In' i*ion .'1 . ` i- . ..' . `'.j'. "V r - J * t = ft ; -- n I . c lfr v ;# ! ;* '> '( Nt'-in r*' , o i t .-* * r: < r . -s ; ; . . , >. . . . t ! it - ' ' t f | * 4 ** \ ' t t ( ;r- . - .. t*>- I'.v. ti.fc'"vt* - 1 < 1 , < < O-S - ' . * t <>* > f -.it t rm . ( . : ' it ! - ,v < < 11 r .i < . i - ; i> o,;)6 'iW.B^!iitSWj.' jjy.HjlU. ,J CI.WUI a f`' w,i* ovr rrcia: * iji.it con:<ntr4 liwii *crc cptiirai.. It J >k .i iu.>' < .`ni.-ntrfttJ o hiitiir. #*t to cf'rctjvcl / rets**!* .vrm f i or*- <tvt !M i! ;< Cor r:<rt -.hr 1 i t nn.', tar. u-:,/ , t!v It.-Jt an 4 l lit tft IfaU- tnu<.er 1 ah i St . a . T!. i * rvls? ct>;hip was llso further i:'.5utei! *t `till higher MoUii crutcessf r*ti>>ft>(10C0 ltrojr fr liter) wl <it iron > i.') io:.> : rvt> t to I.5" v.:ntIi jf r 1ltcrt?tyurej ' '] 1.},. VJ.i'.c *i i oj*e vsrtali'r. > r. t?>c eapr r im-:- ta J data, ; t *< ,<!." r` ' t-1.- ; ! .' Jif-i by 11 . * i n Is ware >*ta 1 ; ', - L - ; . : r sf > : V. ->r ' f 11; ' >* . I >, y-* .i :,f j>(iv i run crvii.tr: * t v i: on, tvHl jer-*.ti* ij of cnurie coni itituh 1/ lejTcrrC ovrT 'll', it "r.o s rtt," * *Kiio Mjjil c vf.t cri t r^t i >r. ->i i.jc-si 5nc 4CiJ ire c t 1 t jJ ihitir.j, troy art <t t.r.cr ni.tr, l rer. it aMeitt froo ;t (tro-tii ;!). Pi:, -ily, tite rfK'ti of c .t f re t orn or* tl.c It ji! - tr. juc ed t r f. > *; i T i rr n or fpt, <^>*_crri .ic. *\"> !r!) ntuditil In evcrj . s : : j. i o <i*c rtu^.t o( i ;> i'v`-n , -. fi^cr** 'f in.' .* f. . ' sw., th.' i! :*t. It itT-C -ir. Ip l e ith' i -1 .. vf. *.I .; f j-rr-.r rti-c.-.ufi *,rt. m <fcih rttliu*. I . 'i:Tji rr t\ ) ; -1 r : i- t t , i-: : v n 1t , ; ? ! .tj t"*>cr t -t j'-v-.'mM*.* t j cone! t; * t tv 1 * mat tr.* i ,-.!i; i> i lin *f. Rrotl ' t . . . , *. t < .*; ' ! .r \ ? . -r t T ' * v * t * ;, t s r * + \ ; : 1 i.\ f. , 1 ' ' ` - K - , - *- ; rr . i' - -* r : . r *`Tc V t % <r : a I rr \ I C T - 5 T *' t.*- >* " t l I I Kit !* -, Ar ' . vi * r .* f* I t *.* .t ' ' ` H '* t r! 4. t : r.tt r'r. r t: c *-,*> * fntntUl ' i K c '*-;*>>> t r. i i ? - , ! -? * t i,: vri'r; t * * t [>rt):iiUr i' Irrrr! f t w.i3.VT-i' r>]1- !yv Z+. *.* fr/w&V.T^ f-<CrVy #A?tev ^:\fT j. r- Aim *, f4 -3s*j? -t +` j^%W%. * . "->J7 J&T-3 - 5^4 &&!&&i r-"J r `V ' >j,iitsmvY' f*i4S *t*l Jt ktu*m to ncti'/M* fo*t of rnt/rejfJc rraciiori involving t ran* fc rn*o> , J.ydto] **e , l>acv, !> '- ^rnc % , and lign-ei, and *ar.g*r.c(; lias nl ready beer, ihnvn to tc : : e.".>?lji desert I in lha !>lt>ln Jrptiiidir, c pyruvate <% rht x ! a ' of cMflrn *"i t-..rlcf .-.i! likely rn analofoui ro> :n other A-.t a Itrenty***. Hatigve.eve hat 3l*o born found to (t flral? ss<.'f i*tJ with ribonucleic cil isolated fiuo direric sovtcoa .mu, in i.' A, the /trial nay < e rve st * l.-.;*.c,-! ir r -.at of Mni'-fiirc art.? If rmy also fonctiop. in ^ruun vynfhoi*. Vii.ijAfje i . i i 1.., tt-v> af'.T. [ .. t til a t e J to nl-* r * in At i d j ic , > r > ! <t , *>r. , jtetal el last <fi fait*- ar Jv , Mi l in' c`)` '. .- -. Xit. -I>)' ft % i > . loo .olfect of tMi c a :-.< > r >t <> , vt; f vj-> - .' i ; i %a * t - i < J i . . it ioi : v - , #-,'. 'ii >t u>. 1? r c*rt.l- (--r.-H ton* wi-jrioif nlf ut i i - * - * i . k ( , ; ^ . k rt ' * C: TSe '( <' * ! o>. the trcwt-V. am* :..e l.-n -n.d,, vv, l : ' it)'#. > * *, - . .;* x*> l*s". !:: i.'.s ;ifi'r ; I if**., t.> $ l- ,> rt > 'K''' : s' '."'liter if-;, i * 'Hr*-: r. f i *n an-* i i f. i , t| .tji.C'l 041{ . i."! i >!,' i 'll'; ^ -i j *'m# / ;o t^* ` v r ,. r~T it # i ##-*. 'eA i. L .; , 1^' vt*w l?; *# u ui)uni|^yajp^wi mij u wuwn.'--jewiffi " 4 'r ' "ft ' j-* -i '`apy*2t-f-ihi. A . im` i>;>>/ t\ X* ;^ `j ri..!'.cy t*r the t f rev-tli iKhan-,i v i n Cif*virj tn t;. 0 1 rft 1! 'o;,pi'vp-J i ren suf Ci - * * * 5 i<p-sn 4<1. t 1 c a c ( 5 ag/t < f rarw r-ru'-M' , r v in i-r i*r J !! r * - .1 i. 1 !*.- v< j. r ff.!>. T:-.is c t' fe. 1 i* 'Mrr* ^ .r.- >. t riVi r. wJ.rr; T; t ii' sor-'r.. >: at ! 'P na , refl^tl iW.l p -jf 11 r>i, . 17 `. ' P* g*ri? 15 4|f >' .I'litMi* ill (Wc-f:rsUtr, f it tK* i'piuj SM'-.tificfv t'Xpofvi colli. I x ... i. ; * M'vr-1 r r. t; om 2/ : r<vv 1 . ,, t :>i J s t *. r ~ -3f > -1 i. :- i` ., > >, . .! c ! I. i r > % Ht t . i I 1 > r-* ' to.;. i* %: :;<> f .. < f l. 1!;. vi liPi.,'!,' "fKt, 1 r,e .aiil'niiiii . , t: ' . ; : '. 4. ...v -., . .. , t. . . I ' * - - - ; *- i Jj .f ,-%'* ; tr !t r; ; ; l`ir : ' : * w. I * J C l ! ! r f. I : . *, * fI J ..u'.ii lead to ,scfti.i4at)cn at <vi: cpv. III. ti tin? rf.'t tr U, ji'*: ji.,i fti j!it **. t I ' ^lisulsfr tl.< j.*? i'u i i! invoJ\(*4 in 5itr* [') iur to thf synthesis of jprr.' nr*'; vri"-.7;t'i! ftrid increasr *he<e preci-rjors an*! <upro- no ill irfi`Hv` the Capacity f^r farther fws t Hic li'* `x "i'ir |){.c. ?','cnf.;i he if the re ted to t he-ii * if retabol i te which .ordirtartlj served r .. : at fi *i acti fe mhitor fer the *,, : ,i * t r *| 'ft* > ly : ! f . f'.e wO-. Vr,- .c-i v i of. i.i t i ; S f- * it : . *i it ; t :h. - j> i / ' vt * . ? ;.( \? o.' - : _ ,-il ."c-th * cr t'-e , ; J ; * < . ! %; J ' i l t \r >/!.* < ; v i*. v * ' * . * ' * . ' r , 5 ' " , ; i ' i v. . * - -* - : - - - t- r-- !-1 v ~ ' ; ?;t, '. * ' - "* * l i^tltggppi J i "'1 ^ .:1 ' '. ''Jl1 l`Tr^~~^"^"'' n, '* "1-- n.|i||i|.n -pir-- I "> . jyWWgurt^ t !t:n variable pigt-eut product!on ight fcc expected to influent*' the at-iottunc/ neasurcasnta. Patarainat ion of the dry vrigb.t ..f f<lls per unit volosse of cultures ptovido ,uch so'inivx of total gre-th but it quit* lir.e-ennsuriing. it va* found petit1 !<* to ft tain extremely convenient estfr-atei, however, by .lej.iureren! of the packed cell voluac after centrifugation of the culture* In WintToho tubes, quite analogous to the procedure enpioyed for determination of MooJ herit t crl t s. The nu'rr ! a 1* on of p*cbrd. cell voluc-es. e'e tertsi nod by this neans, viih the weight of coll* after beating to Crynctt si J(M* 1* in ligern 51. ''n t S i > i.;,>is, a i11 r of peeled cel! ve. lueeJeO') - * cor:cpotlis l l.??t eg* of salt-free drtr.' i-.:-s -.,uc 11 t-?a>or* rer-t-i <i,k r-rfe r red t. i`,;i # f rnrtsa 11 ring thr data. . f }-. f->; rw; . -.ei C, If feet of Cord( r J nil ,-f ..if :>{ 1 ji.id** ; i * -! n btrtH Tv; *- f < ; t of n.,j-f j.i-se l OS; a t :* t: ov- n :; ~t- f <. * _1 i i V, thr-** eft f fefenr mro-t. trs!*^' f r ' ; ` > . _1 - / 11 t I ( I.T . I it!'-, be !)!.. ' j . : , t i r i , o ' ` -; s c . ; * < . i ' ? l . ,; > I ; - . ' 'i, ' ; ,*`;r r - r > t ) * i' t 1 ...*' V . V ' * , . u ; . . t ; . t < t ' . :rn r * ... .1 , f i , .. . . r :' f.. . - *- : . :,.jt it l; ; Jrtu tc **'!< < 13 t'.e jirc>- t ;t i s i-l'. i! 51 1 t'-fl 3-.S{ rojtJirj ir. The piwr.r .of *us .trot.. -*j u > i .,, v' ich f'ccnnu;', 1 j;t inj ** r f'* . '-fH * ci t!wi-,ic < *a', ch n (''lnr.to'i .hJji has ia c i-t *<. K-.<;.'*, v v jsm ivtkn.'A 0revioy/. ^ii; it r<--.r..v to is otrtaifsoi f ,,ny .tthr;r -.'tal will (iihstitHU !* t* iattf Hvr1 $: i that i-jf.. ' l f '< : i , ; 1 >i c r< *' * f-*c r i *i. f ' b \ Thr ir.` i ,* I>-' ' ;hi;V^,cVs'. T,".1.-*-t*24?^.'.,-j^Vi*3,ai;>sJ. ` '. ; * r *: i 0 i.. 4 O' 3^ - r Cf-ptriti.. !. t Sit*.: The `?frct ' { In clfC':v! ts ;a figure.'34. 'Conditions tr ns it , Fis'.srr '* j V'-t than (he prw.sencc of 1 x 10'* M Mi acelate. In goner'll,' if l *r,r3*'*ri{ that hiwrr c ci.crr.t t i ons of %*i {.j>U'<e :tv required lo Indore ccf.n porpli.rm f 11 proiinction if lemf l p,-&r:it. Uni*, In tultv-r.-'. '%rown in the presence >f 10 *VI of iron >.,ti 10 of Hija'ieie, cep repo rp!.y r i r. Ill ; re.-etiw i if !< i! is present in this i wv u'!ur-, l`.--irsti . :.-r;a prol.-.t n is c : c ! f.ur ;'. *.i-1, K . ir.'. -s > ; ffre: ii. _i_i i i f f. * v. f V . v* -. i s M r: c : i i x- -Z*: 'i * - i - ^ **-' <`*t 5* f t ` * : r , ^ f. r iy-* ,i it'- rw Jt * : - 2*^ ;;^U t^frn!;.p ;. . i - . ~v >vr- i l **>;*? ^ Tr . *s ? * : ; < *1 i t>f V,'* i 1 - " * ***;* :"* r * t! i i * < : ** i * * ? ^ tp'.t f i it %< :U';, ' 11 t r . t*i J ' t Jr i f, r f* ; 2 CtV*s* . riV. Wi. # , r*d } 'J ^ ; , i' 1 ' ; , ^ * '* * fi Pi' >f : s ;; e i c*r ^ f ~ i -'i . r,^-; .* * t/, > ?.*:.* i i - *-.r* . - > l h i * * ' > ` * . ( t - ! ' * s f r i i i t .*'. * v ' r' * ? ?' i * . i i r > '? . /* i Z-i*- **3*. wTgjwgggy.w1 tlo c!:Vct of i ron in th; .Tfpri cppoara rather itloinn jawfi in the j'r*r:;c*i of )o*J ! i .* fi i r: y at* iilc^tioai interference with t;? te rioch Jo rojtiiy 1 i proJnr. tie:. when ranjcaiio it 11 v>MoI. Thus, _l T * cn_ ! .* -.*< of these t- i*><.- t i j!ur t he mgr* a.cj ercr-Mnr. af coj'rop;- r- hy ri.-. I ' 1 i n to the_r*_fj*_of ?V? . c t'>1 -iet M.9K_1_<n the rrcien.t of tunjar.rst', cn/t attritotrrf to a blech by tM> element in the 'vn_t_h.-t it i_ h-^verc j to ts*i fS 'Jf.l hoctv rUc\ i_"rv ' 1. ar. ' f. fifret of cri !h;i*c Vic<iijc! 1 on e f f.c i >'. .... -c -r p r _ ...... t ! . :! .... ,t ' < ; ! i. the '.it ? /, .* ri :rt: ! r! ; i-t . ' : i i ft _ n! t i t i o;, i . T t < <t :;" ! i t t ; i . i ,* ' i i J ij t ; ;;.w iter !.<. ic *:rp* ;. t ' the . | *1 ' i !..'4 ( :r, .if th s* a! ii in* j->r;-Jl">r > " ; -.i> frs. - * -jf .. .ije- , v1 . . t r i.. f , o t f f i r.n; I. . i t r ' v i * ?.* < r v I - -.0 ! > j , . ft >; - f>,.. h>l .<. c t -.. . I r.<: 'ts'ilt. iro rj't-nn if. Table iV. l lthrr in ti;i nrcfzutc or a! jrnct cf oc-iecJ i ror. , or i-i.it. ! Ir-'t rt- v U j -1, io * d2 ?.<;.<* in tV- ciiiiuro* was i*Ssu. -3 > *' - ' rr 11 &:, 1'. the rulturrJ without auJe! iron, hfvewr, a.| 1 it v r i-< , r if c,,-s .- r` ti-r< ssilo" In t-.cn* yy > ' l>. - ' 1 - , , . . .,, - . : , : . ` \' - . ~ "r . v..'. i :V*iT <'*, . - t.r v : r ,i' >i -.o ' V.- "**;.* !irt i > -r ..- t-'.vr *yr. i< . New , to.'Vrr, JO.!:*. i .-t. i l. ? K- re t'lA.- I."*. Tt.fcH * ' 3 .1 * T:. t!.f .>S'*?rvir-. on c o j m op o rph' - i.r- j-1 t> ; .-.iti !. S> r-c 7j; I t ik-kl 1/i - > nle*.e is >.t * l t ;t s i *{( :?. . ., ' . * ' ... v -v . :: _f . * . ; -, * to P :ri f*o wit.*.' tatry t . ts ,, .!#> f t * , i i .. ( */^i t 1 1 ' 'l ,,! '4'. \ _ (.. i * * 5 1 i ('- !. )- *.!* jtCw-,.! (tier, it. li.-f jl *(' ` i. ' .- ; . ? , l f r f . ! r <. *!, !' !< * t * i ** i ' i ; . , r ! > , . r - ' />- r ' ' < '- i ' i r> I i <- r* . .i-o! -...* - tit-' . t > *' ` >.- . ?k:>yo-z-: .......... . ---- t , a ------ ------------ --------------------- - a . . - . --- w.^wnwwp^ , zs~- '' xaai *--t*.*xr .i<5*. . 'Jii * .vr.cs is c >(>.: '.ecu J> `ril*:r than for eut-y nt.-Jii, r. jt. , . ijt* *, or Co*'. Irad 1* a ..*UUv:l) ..* Sr.hiL.lfor of i nty*e&. ' " i ' ; * ; *>r > r ;vxs utc ?M>' f ', y o *'5 !>r a ' * ! f ti! -.*)i)iT''J' ifr t: r V th ;i ;v ft 4 1 n f * h- o .( r ic"* n u ` I*'- ' ' \ * { >. ' r` :? **!* t i v; 'a1' . a-..! " -.'!> ir.:<A <CJtrJ lillf -1>. Or. tr* i5 Li -t'. it *! .r. i * ; ;-,,i t< .U *' . it. n`. fe u *. i. i. on: / -1 vt > k S ; ; f 1 . . ? ly , t iM lit y Ki * V > t 0 * v \w'!i ' v*s ; i > , > T .-* t * t ti * ! t r : w r r '? ' 4 ?*- > t * '-l F i *' . f : : - / * ^; - -. V .4 f - - . - r H - . : l- < r : ~ *> . ' ' ' ; 11 . ^*c ! ) < ; .! UV- :.*;* t \ ?m, * . , 4 . - . - ai y * *' *T K * ..: * ` - '.' v -* J .:. t - V' it r * t * - " i. > , * ^ 4 '' C >. \ * r . t - : : i - t 'if nt: r >; i ! , .-* , * i y'. t1 - ---7- . It 5 j- ' i ; ' v a J. , .. *- f:- 1T r ;V:V' '*' ^ 1-_L V 'Va .---:-- %- jk3--.g jt'HWijjumwWMjP. HI-P1-1 > |Uj -gm *';'lT^f* ^ /y-c'.v * C?w, I'r^'nffc'iUtifeMiiittihis ** `'J i^..t ^n*--- ' ai3A- ' < f; r \ ' ' %*; 'i - i , 1.1 i - .. i * t * j-rcwi,, t* *i * r.j t . : -rf t :> * n- I : -J ... r^up j' Tft.ir c*v.>i./iti iop -r -\c : r.*; , : .5 *i!5--- <. i-o< h s^rt* \ * w t > I. I< : ! thjt. s \ > r f `-M' s-v^ , */- 1 *' i r ' *r r '* v i* : * i r'* J M* V~ -* . 'v "t ...tur li i. ;. . . i < ?' - : * - - > ;*. t, : r ; I : ^ . - ; . . i . '' ' *r ; t / ' - ui t > . '? o t i i f V V ! j i u . I vi r -f '.-if, ;>* r'*!' ' , .*' .nil', r-' *u. * J I'rr.i r, Mitr i jv t ; n of tSo :-;j i% W<- > .:/? <. r I.*:* t i . #v t i I t / To *vM l-*ij tVr l M<? r*; T ^ r,*, of lc*3 v 1 O' t\l% j:t r. t -f frIi c t i'?*> of Ir-crU uc l J ? v ; r -IU : f i - ' t >. '-t# - , 'I 'V PS2^S iwwvgr.'WF.T^r* UJA"1 t..iJMN.!i^JL.i, I i; m\pm w11-- ' < V * C fv*- '1, i K Ji CM e i! ftf l.f'w "olS &f ?*J of tl.v- jui' : u .'C! ivlti i; :' * r-ftscf.cr nf b. ; ?. V i,L. * {: .OJd t * * . P r r:t, vpQt&T* to K* t i r.-. * .'c "c:i'.:'-r , 'j *' .j r>f? :: .*' i; j . ~ ,r I'viirr - i>J ;r.c issr.". 7!u'> Lf <,! of ITMI are e *.-a-luted A r;:vot.'- After o' r* '-t t i r-J' p Xi r-th*' j of in*. IM * . <,* I * .' `f'.d re.sril* ft ?*/ tonc<*n tT* HCtj of i t it* "`M. frf* t'ii . r , r U: s, l ` . .'"P i i t " r -T. :! tv ! tr'iu >{ t* > ; V ; i.f '. r(. : J r %* .. *?.* ' '...V. ! J> ; ^ 5' i- . 5 *s t \t t- * - t i;.o *:v Lp.''^'-.t ~ fP"1 y .jy *yuiwi.WBJwy.*' i) i; i fHHmmgwiwqypt .i. i.i,u * tp*i i.% X-U' BWKUM*! A;tii l;;;'vatc, only 'ui inhibit ion v*; >bsn.rvc-; at t:# content r4f ton of !}>! 'irodoi*.;*^ ''' * * l.h i ft t t olt w-Str. th* i>r*V / ->f of li*ACt.r*tt J*CYi ft 'i* ,* :.'i- f.;vnt ;> , th*- s T'Hi! S t tt` .u, v 1shifted te*r. "-l i ;!.< r l*i ,v."f cr i a* ici-'. ccroting rvn appa.*cc. *!o.cr fll f i'" of l^a.l ft,r Mi-f, 1* u*, t>rrc r-' v;H> tler-.onst rt- pnf.il P rot *' f t ton I; . > t rtf tC% Afc.ilrst l .1** C t l t A11 ort of :h. V. :-Ji. T'i- #HtCf '( rtx-t.zrf'e' An. *sl < t r * t os on *\t ' *v< r* U i * 1t- ,. Viii on l>' l^T* .< .oviJe* .M> tir- ;l <-v. j .-.< i- . . Viaai it l;t .-.<ti /o ltc of * ; %e,v*- .* * o * c* , u ; ** * , ` 1 >v / .It- < i: : on f,; * ' *f;` :V r J. - sS'Atl- I..J > ,f l I ' I ' *v ' ` / t A A * ?' l . - '. - ) > *':* \ IV- ?if . :n 11: ; t s *> * o v- " K* - i SSv SpIliVllP I*-.1.1 !JiaFHBTq it yyfinipi if HjPg|lMlii 'I ar-n r-yry^ agysay * 1' WW [|C3V;*{Vt*i;j **'*r'' -*v t i 1 i Cm; * !> , a v * j v <in sli'p ifthii-l I lvt; 1* &i' ;j*v rv: . M''. f`. V[ .|S ,, t inn; i tO r of \* _n Lt*f * rc^i *i**t, I/;* V ^ ri ll; r :i,-rli r/ f~>r t t ! i i: in .: *.! ' .< * . s *;: r vV rC r:'.. . ' r - nji^r ?rnU-i t 0 ** t.iAl. V: id. A ,'u rJ,c c o r n f t * rtv Vi : f.r, t.y r.ljj ; -jr is it5r r*' .rr >` 1 jr;<j t'1*# '.r c*ivo Cv!-Ui'? ft Cl h ' ! r _ ' * r- Irr wirh .r- rr.ryx"-': and *>:M t rate) I }< If V). i * *\ c*^ f j c i d an i . ` i.r- c;,v. r r * t * -* i , x > - . ' ; . * .M f ;; <F . i ? * * / . ^ - f > -f J ? u i . ? * I i: fo : - V! '.t - .' i : i < "i T-- 'i'VX - r h * ujuMjHuiy*! ^3* V WiUjrygwftMjg ,3 thfl atio *i:tw,:. ^;;i*vtT-*- of FAU Ku ?i ? u 11 4 t* that of fnc f>;)( i?*i;t it witU tho t-tfyi.j; *. *3 c? t: ion of ?/.r> vitjl tl j>f< t*i n, Hij s ii i.en'i t Cl. f r< the Mjj',j;ei t i o'* t '> tt r r. ?**/ *' *'i th Xht >i!. thiol /. v v.r cc rt ir. .'. -- ; ' f rA C'i' , Tj: v ! f. ;y, s? , V -3 :. ?, a , * ?, 7`n* h> tif :1 * Ch sstcjl n?>; * t' l i * * c f 1 t prtrfpj i' &j i<! i1 % inter j - t i* i t; i < a A i - J t:nh> r c/,`! _T ,. '* wAi * r - .t v. :hc C-J-- '. : i' *- , lr o;ir^;'. r..' i> ;-<VO' ;t .< the / < > ,n ). ! l * to nh !' i ? il>n h. t fe r \ '; ., . .. , - > w ' > . .' *? * v c 2 } a *. ;. * ' `. * ? , ' .-... . l,o *.: 1 .r , , i. : . ' v , -.V *: , V ^ ^ ' > 1 r. * * 0.- ' ... ' .' -# SAJg; Tkblt I mem c c ml h i cr xirAUOTHjcwix rx ncsm w j it k :c*t s CCA Cfiro(Mtc^riphy Ps*k 1 C->*1un jriisur?**!* 230 Ztn-c 540 J*ti It 5M 1M3 ESI Ui-% :v?sv=>>.'.-r j ^:m Tiki* II >1141 Ct*T*T 0* KTALUWmia no1 lE,\0-rOtS3D >WJIT M.'rn$ &.tl Chrt<e***vv.rr.'f Ckja'un ---------- -------- --------*177* proUln P*A I BZ r-ii it tjnc Vr,/y' pn>t*1 20* nci L^t4. i*c/-r IOC-5 m I -.. 't'tfw.t 3, l Pl-Ii J ` Sodl'^vL-glutiflrtU orohydrit* 389 0t-9llc icld rMiW4 W (KM4)^HP04 2.7 9 600.0 *? 5CO.O ag 000.0 *J ^504-7Kj 0 c c i2 HtcotlMc dd 200.0 *g 43.0 mt 1.0 tn TblMln*>HCl Biotin 1.0 OJ 10.0 mltr to 1 1*U^ p* 1$ to 6.8 *fw 1 4 H*0H b*f-r *locU1f-7 for 10 olAutfrt *i 10 lbn/M 1. SUftrv>Cf. 'JHilltl, 21x^v J. W. 70, 1164 ? i rc-i pM' i: c 0 0 U Hie l > ,y * n 0 J r !,ri.: k v>- _v. 0 I 'tt; . ei,/nv-- 0.77 .7. *4 C.-le i'-H O.T-4 t.'O n. <ij i y U'lHPta^WWP H4W . i , EPTA.M Table V REACTIVATIOM Of LEAD A'.'l' CtPMlUM CCVrLrxr.S OF LIS'OAMIDfc !'l.Iirp?OCL')A.SE (LAD) WITH LETA Rcforerce: 1RL :C`)-27S) (.'filer of Addition cf U'TA ACTIVITY l I.ADico.itrol) , Cb-LAD* , Cd-LAO* - 3.<in ' '.ifin ' after C'.u'tryrc* and juioirate 1 * fn i e f oe/. t > ?v ,.>'U 9i IOC 1 It T 2 : ."if* \ 3 O4 , A v v * A C "f * i - i * v.!^t it l . i * v* . **'< , * . A A.1 I'CHt. i * * *'. '.IpOJ't, j. * i ;0* <M; 7>i}S Cl', O.iH, p'| 7; s f ; TA a ir.'l t > ! v J. r.- : a d i'ir, <: ' 1 : ir.',' AlinN iw '* * ( AD ui ih 2 * 10 4S S>b** 1 it 0 1 > T r 1 A r lie . pii 7 , c .t t \:> prepared >-? i-tcuL.itJOi Tf>* -olvn pC l. I VH * i !h i * 104V Cd** i ft ] J 1 jym*. y-j oo Crl44 250 In pprr* 1.0-HX5 O'f Ottuilviyni'Uy - Abftf Ci/ finKht r?fbr*.itc&. Gf<t.323-'>1 0.8--0.4 0.5--0.3 0 0. 4 0.2 0.1- o.l o 0 UJ *..nf>n. gj.^Lwyy1 20 40 60 80 tOO Ho Ho Wj Tube Number fiqur<* 4 i p\yuix? i.iPuii|iji.njUHtii t.y[yuiw ryiwr,*y > wi* ffW.m i'1gpw>j- -******z--xi`-ru .v* a l?ggwffW"wWi L IA 12 268 * i run i'i wnipii j~rriTprig-m !f|i>|y nni~r 'imy i LI442269 t Scphodex CHfoi^ato<jrophy /eatf Ao/so ne</ Rabbit Rir/neyS ji i I ! h.;.V tw <r? C$ Of P*> p p Cor >i O Chrornatography of lOdr.cys fron- lend Po^orc ftabcc: -DIAL Colon, f- \ 1(0 t` * w. K ^ s ** -^5^SI 3' r ,* - *nf%z r "* ", ''* >' '*>?'*&**\<** k7 y^ *- v>x *"** " ^ ^-y*n "' * V i>f CJn ^ 4 4"" " t +~* ^ ~ - .- - -G, \ ^ * V*^ nk \ * - ' . G. GROWTH CURVES FOR Rpi srl^r^: (sl'jMr/rW conjtUoi.;-} real's;F'Crt DHt 323-^1 Fe locyw/L'.^r J0.0 fo b/j M /J rter s ao 8 <>.o AO ;.Q 0 J______ ;o 40 MO'JS J v* ^*:v 1 J____ 00 .'i.n^n.uw 'J gn ,,._l_____ CO Kin-ir'; r ! i:; ' .,;' [ r FECT Of IRON ON GROWTH Of R>-5 wh^nkits reference B*L 32V34 N, l-VUe. A 400 /otin 10/j^/i'ter ^4----- ----j' > GO o < ro ft- O '> cO < 40 /ft 0 io 30 1 X 50 IRON, XX V y) riW/l'tr,- *v If- .1 ''yur^ & vyu a>* '-'i fjiw ".*7^'W ` ' ' ' ' .'1 :-s f'^Vi GJWTHCWES FOR RPs spheroidesCtfFeotcf AISOM* LEAD \2 Oi-- reference BRL 323- 45 30OyuMFe +P& 3oo /iMFe 10.0 JOOyjMFo t Pt ao 100/4 MFe As . c V i.o 0 l/j M fy OFe I/j WFc + Pt> oFf. ` Pb tyr:.rtv^vw 'fv*? 1 60 ,r>0 If'. 7 ks Pic? * '`j -*.*/ t v * .As'- v -.-ffr? GROWTH CF Rps SPHEBOIOE5 ' ~ Effect of Iron Concei^roHonin Presence cJnd Absence of read Aoetote reference 6RL- 342-4f)& dlollnjtyjg /lifer TMcjmin, Irnp/lifrer Sd-- C+)Pb f^irotinic Arid, Unrj/litflr 4^1 -- 3.0 o < 'O 2.0U 1.0 T' i; ii I' ii l !i I II ii ii 11 n j iii i! il 1 ii to s6 lron,,u.M/Urer l! ii NI 4b~ loo 300 figure 8 r; **1Sy* yr?fr.-.**V!KN! -J., &" ii. |0.0 6.0 mi - -*-. * * * V Vv' ,. '. ; * * -- , . - ' -*'(*&:r'^ M <w. *w ' ' - V'' ' .GROWTH OP Rps 5PH6R0fDtS Effect of Iron jr Concentration }n Presence andAbjenca of |r wlO'4 M Pb HI Acetate Slotin- JJtig/Liter *. rtfew.-SRUiMM Thfiaminlmj/UVer 1 I Nicotinic Acldhflg/l ^ |, j | - JH3f-)PI> I 3(+)Pb t I Fi--'-*.--' fa-,'.'*' A';-">.'.' * ; A-V" ll 6.0 IT C 4.0 I! i *--' vrF-;^t's T- " ~ 2.0 Tn 0 A 10 50 Iron, /jM/Uter ...u iv m;,'" m,u -// \oo 300 A^qr* 0 n j* iV-^' fe*' ^ >^*j ^ ^*H*' ws ./, 5i:4-,--^ * -V.- - >wiW. .- .^il.' ... t M-- >>r rfAtmSi^-Jkg^r v .* ' -* v. V-VW ir i?cv'--^ `O*y*v .V'^si;*^'; J -vs,..!_) ,,...-j. --. n,v- -^?T -.Z "i'' CiKOWTHOF Rpj SPHEROIDS Effect ofCoPqctors on Growth reference -6Rl'323*34 CDC fe* 10/jM/uter Pe-ioo/^yLj ... . ;-::" 0 cxi i o to yj k o Wcot'nic Aod nry/nmr Liur f^ggyya Biotfp ^yL.t ,".iivr.i|u|tji,''w ficfiU / W & GR.OWTH OF Rps 5PHWOIDES Effect of AJron vs. AT7iinnfn in Ppisenm arid Af>5eCe of Lead reference 6R 1-323-400.1; Wico^infc fSf'irt IrrtQ/ljkur Lend Acptate iylo*4fS4 rrjwi MPb 6otln lOyug/litA/' 4.0 C+OPb \ f ipMJUMW. *^<.,firriirwr -* * tH-HM*-!; ' >^;U - , . 4k- ' \ ' ~-S& Gk CV/TW Rp; SPHEK0:C*S.......... ' '' " ^`f-'.ccc/ Alr^vyj Mlv'iffti?* >r> Pj^ftf r *? tjiifi At-*cri.> c> l-n`^ 9 or ~ fzftr+r^fi. ORl' 3D~4ftQ-F WroN^C Aci^j%* frr,/f.f/v 80 ?i --*(-) Pb *P L^.ad Oi ^.<Aot*i ot in '| <yio'AM iO/jo /uhr I! !! II 70 |l ll ll 6.0 |l 5o oQ iO XT 3.0 10 in ii I h> O'fVn ww: *"vgA.jw. *}> ^ .I-./ rwg 'j.<> jtp 3^ il U 'tj ^ WlpH >>J_. .*y4yjWXJJj.ig.<j*Ft ^s.^yg;r'p^jfti,i|W n^fti <y* ' u. OWTH or RFs 3PM F [ffecr of A \rpn vs. A Nicotinic Acid in presence and essence- cf Lead reLV. encp Na-325- 40 A c ^ (-)Pb 4o r-s<*' )Ftf TV*)cfv'tn * Pb " ! X 10 ^ M <Oyj^/Lifer y m m. LMII MU* JW J|L.jyg;i.^fu.^jjagFygg.!!^*ifL?- **v-i ij* H t\C:;Ui'5 of-41ron-vs A Nicr/t')*jr./'rl/l *. prc^e;n*,*vy? ? I w'i 6*) r.'t hianVn . H: * Wl'V'V4 B' ot,r> ' r -- .-m j *J**m.im { *~f13 I i !' !' jl M ;i; *8.0 i* i` |. ;i iihi i* * . ; " I;i 13 ii i. , {iU'tj \ f ! n U> \l j .l .' .?r , *w ,.wj i JtjjS ju uni* jg*17-1 g m. ; & n i," .Ul 1 !U `-'i i ** a 1 .-j A r**-\ 'piSRsfriS-M8^.5$^^ ^ Vv ^ ,,-' itS'w./k Z~-?& :r* s^-isf fG^*" > wr /I-.'jf!J5^ **' . V") .. , IWr .f'f' ; t >u' y ivtoT, *, ~ +~/ . " `, ^'- , ^- :>xr-v . *, KVsf',, V * - '' ` . ' ****** GROWTH OF Rp>$f'HfRQIDE5 Effect, of Aliens ABiot'in in pt^ei'-ce 'jHobitnceo^ {./*# reference BRL-3?^'^ 0/ = = * * (-JPh -J~*ssssss# f U Pb /V.e ote ifZ-'r,! K/i'.-;t;rv:- Ino/'rLU I i* *CfA> r >rr* v / l/l'r >1 0 3> . '> / i < 14 I in ^i; ' ' :i .: 11 iS 11 !lI MI ! I* I ;i i !. i* i.; ii ! 1: 'I i| :p '* N >: - V !i1 i 'll > ! V2: :H: 1 ! t h '!:! :r* i K; 3 sr ! '* *' ti**!j L* ,^ T * *t |Mf! ^ 5 15 > ` r\ Ii t' >; >* ',.7 , ri, ,*. O : j*; "il |i 'f i!i!i!.if.j !ij -iP___ Ifi ll : i..!. ,, jt ?> tii ;*. 1,1 f' 1 i- , i ?' ;i ' * : ^\t o' 1 '.ft, i . I!,,'1: \ If ;j ` ! ___ii! J ;! O F f' - 1. r rtyyratyj^pywm p^yasi liw^wwpwwwwyg r^r- y-r- ,f]* * '*...? ' .'. *Q8kt G ROWTH OP i>,5 ^PIU;R0!05...... . -- Clf oF A !ror> vs/> hx>tm in premwe > iiv) ab&`'ce cf Iccjd CUwm Of %I>: Siyr'lRCiV$ ................................................................................. Cffr ft < AI rt m v> Ocf'-'l f O *' S 11 rt4c;i(`i*Z i`.Kl `>ij~-4*1 KT n: o tl r-e r-y <f 1.-0 <* l-'yl v * 1 XfO M '?* | y i w y i, l|..l y pyiW j| . t , l ili < * ^ * w W * PW an I. in ........ . SEit r~------rriirnniif-Ti mm**r*, .''tiJz * *?*1 ">' ,.: ?* '*'/'**?:*5r*^r*s'T^78r'*' * '' %. < %, j - /O ^ t 'iy, v-. - ,- ' * ,, ` ' J :jSSTiSgt______ **s.^j ~w *w s bf .,--5 t- "3 ' " ~H r V*-'.- "H v vi/;V :LIA12285 , *% '- ^xxsxesss. *y*?BW yr^n ^:-v ---* .{*. V'1'***S* $ '' 5'i.'*^ .il /"/O^'y wV& ft'iAmitffri t.ROWTH OF Rp$ SPHER0/DE5 fffect of 4 fron vs. Co factors In 9.0 ~ presence uM obsence.ef lead reference 8Rt323~iH 1) || Lead Acetate l xlo_4M 8.0 (-) Pb 3(+)Pb I! II / * iiil 11 !i o o T -.0 .0 l' L o S h Ilf l! I I; ll iw r.` i ip cA H * i* p 8 :i !- irrr y.M/1 tier ti U 3 o '< K ' i* TJ rr> S 100 Figure /? i; . {**** ^ Tr1.* V .: - f v-:: K5' *V**- L IA12286 - ------------------------------------------------- v. -' ' " "v-;,. . I LI A12287 GROWTH Of np'> S^Ht!K;iOc.S ............. - ... ......... .... PfLpcl of H">K. (-oncer-1 i tOM,Ot O OjcO.rs- A'o hnn 4.o :5.r* n Or- 7 C s.O (y$ 0 If. f|! .1 if f-jff-rot rr* fcV*Cf ,,V4< *5* r r- W-) ft' m II T22sv?~%< * ) ft li Ii |l 3 i5 ii il II I:1 ii II il I* (' II ! H1 ! ii h II I Is l I ii il il Ii !l ii 1, l! i* 1' H II II II II 1 l` in I 1 0v>-. A If t V -0 a\ <1 8 if, VI / t* o h V-> * 1i It t i< 8 nt I 8 i tQ* Ifi i1 ,1* il li. <V .V V t ts $ ) I V J ;ii| T i! fill !H0if| t*: U( !i ; U\ . ;*i I VJ t* C A# t* t*) D I I I III ill lf| i ii! f i`-Ud .la M-. 1 MM f '< cj * r f y* A- s^.X_<__g--y&` ,- `-w . :^v ' ^ ^.~t -'r .T, - ' > .* . .M ' VO-* j-sss-!* ` -V * 0. ,, .... `TfltMi +xS* *** f*^ ' *" '*H<5 1 Tjr** *i in *. ***._ 1 , v* " -* & 4Z` * > J * O' * 14 wmmm -y y : -<>. - A -'.-.-*,'-'.r- --..:!-: ,!,',..:?* ~ -v- -w 'a :.-: -; i-. msm?'1* - ^qpppwwpi* j^spiPifpw^^w8 '' ' LIA122.89 1 y. i,L*W*-i> Vi~ * ?*" mROWT.H Of .V.Ofi:LR0/UL3............... . ........ ... Cf^'ii-fc of HiyhTjj Cc<r.entrution Cofactors + lron rcfererce BRL 342-5^ Iron, 10/iM/Liter Ij =^(-)pb I 3i(+)Pb -. *? - ' o ro < -'jf--'V;j.' figure A3 .. t * >* * * / <\t* ^ ^ - * "**** v? Vs . *.%* ~ " "f - 'iv "'V-, -8**~ ^;.. ,v*. ^ - '-*!* ^ **v - - -' ^ - i *&,' ' * ^ v^c* LIA12290 "W1 r*ROWTn of QpsSPHFRO0E5 tfftir* c/ Tli'fli.iin Oh [filt} !*/ * t> MoyO- Or <*w i rrfrt p fit (t 6KL 'Yl2~Ai Vb N.bVuY.b * !* 1(0 4 U1 4 Of - Pt> hoi)i^> ^a *^ do ih 'A i') < LI 1* li I, I 'I 1 :1 |l! I I' I l IC *c i? l Q JJ li0.1> * -4 : r * `c I. ,S 'v it ,*> ;-ii/ C > 2f 'i; l hn b % V ILilL.. Jl: MliCL 3&S* 't, '*V---_ -v : -'-.J V- . * LIA12291 1? ' CtfOWTH OP Rps 5PH&R0IDfc'5 f/rt</Hi|/j/nmooL''OfJ inhil>tUcn 0Ci~ Fb * I X |0 "*M rcfri.c' B*l -.ss*,* (-) Pb SS5I H)Pt 8.0 II I? !i !'! 7.0 I! II I! U It 5.c *! i l1i 1I c. o r i.o- if i 30 li ifc- 2.0 I v li, i .* IV 3 1.0 fi r, .111 i 30 liii&aitMiriii-iirtfflitiWiarfrwin'iw^Mif^i' > -- r Hqure Jo" aifeiiwSaSfK-': *T#* V ' ......... >j'' <:&: i i 'i f -"' HM229? X' gJMWIMi LIA12293 *tgij *(*, ' J ja.jffftp^^gs,!ggaag!gp^pgpwpp^i^^^iy^^ Li412294" GKO'Mi i! T)| !<,, * ;'.rHn*0!pl->' f f"itrt Mu ^ * .4 reference BHI 342*53 -Z-^r*(-)ft> f I` J f' ti /,o 6.0 )y o -o .S ',. I .1 l,, ?:\ :i .\ iii ** >! i'l i` * : %m!*? !* t ! !;f*: :V I '5 H1 i* ,;r 'a... ' > ' If t: ii St f:j iI ii ii! w\ ;n *1 j: <i! If if. * -hV !T ii* fc: I'll l'1 ,b l" *tfi I ! ;3 i rt ! irt '!; p '! pi '!. I H ,, C; ,y. ;j* i- | *l*' 4,1J: .1'1 , Vi-------- * <<Sy 'U-'-'-j-<F'< HPqrJWBWWy rt-*^ ' *>- ,/- 1 <0* * w /*V* ^ ^ > f- '' ' ,, *- ' i. > *<* )< w J ^ j4 * ^ '? -t*>F J?- * ' -y V ( ** v~v>~- : .... .. :*ns-. .>'../ :#' . - '#*r' '' * ' j -.' *V-' * ' ,, ,, V" * 4 .*>*V ^ X'-vW^, 1. jp*^* - y'* J "V ^ . >kJ? ... . '* * -4 "i?-*- r ' 'i-s3p!t<jz- 71y3l SS >; :SSo '3--; : i # ^333 -:*i^is5--V*.yi :?&.->: :; -A t`i3* S&&; LIA12295 ;. y ... !f> 0 r K . o H.o Vv ;-' < 7 '5 *-nw' ,'.... ^ .> >' i- Oi [fCr cl of Mn'M< r'fi'C' f'l'.o {', Hi J, -/- 53 .r.r-v.'-OPb *=** { -/b :i ji fl fa I ii ii h ii `P iir: ; , * !! |i i i' S13 '3 ii' 3; * I, ,u u If f i> M ,i; i*f ii s ll .fi'.;! jit i1 , . .1 3'tis XX itj^ > * . '" iV ; Hi it-:< ` -v o, :' r *"-* 1 i'}: ' .i -ii. c:! SI mi 'i ifi.1 'll:! 'I|.i `i I .Vi* ' * *4 333I" ii ? 'h i .SlJL tr i .** S- 3: 3,3 7' , s* * , 3S'tS- - 5U " S ^.7 " ' '' f LI A122 96 '" ; LIA12297 i ' -O ;' ^ | GROWTH of ftp. SPHfROIDFS U?nrfat ion Of packed, cell v-ofu.M" r^r./t dry weight of ceils Reference.* 8R(. 3V3-?I Sc -- JS fc' t. vO 13 / *V 'c ;l / r? ./" / / / i r&*~* ", ii. : -pf' w-fi.'A"" J 30 31 . yi'i< rjamis-jMgaagioM J : LIA12298 I \ i t;ffi.vT Of HaTM ON CrROvrtH OF- Rps- Sn\tR0U)0 <T Ketdrc/ice .- 8tfl '3*0.'ri. - 7c m> jiwwpgswwr wr LIA12299 *P> LUi'RJt.'S:. EfrT r C.c i -awAh.--|r nilirfriff**1'-^*-- .. N M tXLKtllUf' . - . /:/ DiDib J *r a y /My/** c<x/. IKo/J REFERENCE- &RL M tZ-7l O iron. / in lCC ^x irnn. A/ J * *3'- . ,_ .'*/ ( l%7 D - '*** . i " - - r1 f - U- . 0 C' tr A." JJII np<LP>in#iwi'w It : "w"""v''' - JJWBWWPII^^--MM .'T' r;r-- ^ "T LIA12300 9 - . i 'i j .i* LIA12301 r* K*iiinii.npji T LIA123Q2 W Httrnmiikmkitkk JHi fclTiO*J OF lIPO^MiDE DEH> nRO G EN P S E- B'Y ItRO -ns^ 100 'X- PisAy Ev.'Z'fKE DPN" DPWkUa o c t j e TWS Ci* Ur d Rc . ' ir * o *-* n no4,k 21 * * xnV atM|PH? VRAitC < ; : r f\ * ActlV.17 1 '. .. n ^ .* 11 ^ S . rCT v* KCMd\f K \\ \> \\ *\ *!*nwp^e|pi -,-. .XL. 10 -b !0's uERO Co ^-EK'TKR'mON m 'Z *' i"' 00thr- h il iw p * mm LIA12303 mmm s' t Siiii iT:'nlW. < PART\f\L ?ROTECT\0>J Of UfOAtf\OE DEHVDROG&NRSE BV SUBSTRATE AMD COEMZVMES AGRINST INHIBITION (ifeS-ZlsO LEAD 1IA12304 DiFfiRHNCe SPECTRR OF COMPLETES OF UPCRM<tE DEHYDROGENASE W'TH LEAD AND CADMIUM Ufrl-lSH) iit. H C i-U ft * 10 mw uniiin^uCTnia V- ) WM 'T" -F -&! 'f>+y 350 Fis^ 4