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123812b Factort Influencing the production of staphylococ cal enterofokm A in milk. Tatini. S. R~: Tezeski. t. \ - olwn,
]. u., Jr.; dtsttian, si. P. (Dep. Food Sci. Ind., Univ. Minne
sota, St. Paul, Minn.). J. Dairy Sci. 1971, 54(3), 312-20
(Eng). The influences of the initial bacterial count and heat
treatment of raw whole milk and the initial pH of sterile recon
stituted nonfat dry milk on growth and entertoxln A production
by Staphylococcus aureus were evaluated. In milk relatively free
from competing microorganisms, an 5. aureus population of 2 to
3 million per milliliter was assocd. with detectable enterotoxin A.
It was detected after 4 to 6 hr in low-count raw milk or sterile
reconstituted nonfat dry milk inoculated initially with 10J to
10s 5. aureus/ml. Though S. aureus reached 2 to 3 X 10*/ml
in com. raw milks, no enterotoxin A was detected. Growth
inhibition of 5. aureus in raw milks may or may not always result
in simultaneous inhibition of enterotoxin A production. Good
growth and detectable enterotoxin A resulted in heat treated raw
milk (pasteurized or heated at C5.6 for 10 sec). Enterotoxin A
was produced in sterile reconstituted nonfat dry milk at initial
pH's of 4.5 to 0.5 (adjusted with HC1). At pH 4.5 lactic acid
was bactericidal to S. aureus.
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DSW 379438
STLCOPCB4100210