Document aywkjX90N5LzMvpzJ7gNKn6M

(412) 687-2100 INDUSTRIAL HYGIENE FOUNDATION OF AMERICA. INC. 3231 Centre Avenue Pittsburgh. Pa. 15232 PROGRESS REPORT NO. 4 on FIBROUS DUST STUDIES for Johns-Manville Corporation Pittsburgh Corning Corporation PPG Industries Owens'Corning Fiberglas Corporation RaybestoS'Manhattan, Inc. by Paul Gross, M.D. Director of Research Laboratory- Robert T. P. deTreville, M. D., D. Sc. President December, 1968 02 018 1790 ** ammmtion o* MMMraiM tm tovucmm o mimtmwi mommimm conoitioms U12) 687-2100 INDUSTRIAL HYGIENE FOUNDATION OF AMERICA. INC. 5231 CENTRE AVENUE PITTSBURGH. PA. 15232 FIBROUS DUST STUDIES REPORT OF PROGRESS COVERING THE QUARTER ENDING DECEMBER. 1968 Experiment*! studies on rats and hamsters injected intratracheally with fibrous dusts were described in our proposal dated June 1. 1966, and progress reports dated February, 1967; June, 1967; and June, 1968, respectively, have been submitted to sponsors. Cumulative mortality among a total of 485 rats injected with the types of dust and in the dosages shown, is indicated in Table 1. in the format normally used in technical publications. A different form of tabulation in the case of hamsters to show percent of mortality by dust type dosage and exposure duration has been developed. It differentiates between cumulative mortality versus sacrifice of survivors at the end of the 24*nonths exposure period, and indicates stage of progress of animals which remain under observation. In addition to the above studies, work on fibrous dusts sponsored jointly with the Mellon Institute; University of Pittsburgh, Graduate School of Public Health; the Department of Health. Education, and Welfare, Occupational Health . -V *> AM AMOClATtOftl OR IMOVtTBlH * Tbs* aOVANCIMINT P HgbbtHPWb Wl 02 018 1791 e< I INDUSTRIAL MTOIENS FOUNDATION OF AMERICA. INC. 2. Program; awl in collaboration with the University of Cambridge (British Asbestos Council) have been undertaken. These were described previously in IHF Medical Series Bulletin, No. 11, "Asbestos Bioeffects Research For Industry." Progress has also been reported in papers before the Foundation's 31st and 32nd Annual Meetings and in publications ("Ferruginous Bodies in Human Lungs, Prevalence at Random Autopsies, " M. D. Utidjian, P. Cross, and R. T. P. deTreville. Arch. Environ. Health 17:327-333, Sept. 1968; and "Pulmonary Ferruginous Bodies, Development in Response to Filamentous Dusts and a Method of Isolation and Concentration, " P. Gross, R. T. P. deTreville, . L. J. Cralley, J. M. C. Davis, Arch. Path. 85:539-546. May, 1968.) In addition, the most current report of progress was given at the Foun dation's Fibrous Dust Seminar on November 22, 1968, at Mellon Institute (Enclosure). A report of the proceedings is now in preparation and should appear in 1969; however, it may be of interest to record here that 100% of the cores of the 17 ferruginous bodies isolated by Dr. Michael D. Utidjian? from the 100 a autopsy specimens in the Foundation's Laboratory when subjected to electron diffraction by Mellon Institute, were shown not to be chrysotile asbestos. They are crystalline and it is not possible to rule out some complex form of asbestos. However, they do not appear similar to diffraction patterns obtained on known samples of amosite and crocidolite provided to IHF's Research Laboratory by Dr. James Leinweber, Johns-Manville Corporation, Research and Engineering Center. Manville, New Jersey.* * Assistant Professor, Department of Epidemiology, University of Pittsburgh, Graduate School of Public Health (formerly IHF Fellow in Occupational Medicine). 018 1792- industrial htoikni foundation of amkrica. inc. 3. Further progress was reported by Dr. John M. G. Da via. Department of Pathology, Cambridge University, Cambridge, United Kingdom, who had preaented earlier findinga at the Foundation'a 32nd Annual Meeting in 1967. It now appeara that the precipitation of a matrix and aubaequent impregnation by iron aound flbroua particlea of reapirable aize ia probably a function of scar tiaaue formation and availability of iron in the form of red blood cell hematin from capillariea. In denae acar tiaaue, every fiber waa coated with a matrix (acid mucopolyaaccharide) but few had the invaaion by iron granulea typical of ferruginoua bodiea. Aa the Mellon Inatitute and U. S. Public Health Service aupport for the above atudiea ia ending, a new propoaal ia being prepared by IHF for a more ex panded atudy of fibera and ferruginoua bodiea from autopaied lunga. Obviously, the above results are on too small a population to allow us to reach any conclusions but they do represent an area of considerable challenge for further study in our Laboratory. . * 02 018 1793 Table 1 Mortality of Rata Injected Xntratraeheally with Fibrous Oust* Fibrous Oust i l Glass (Gorski) Glass (Refrasil) Chrysotile (Elect. Precip.) Synthetic Chrysotile (Mellon) Synthetic Chrysotile and Bensopyrene Synthetic Chrysotile'and Nickel Synthetic Chrysotile and Chromium Glass and Phenol*Formaldehyde Binder Glass (uncoated) Glass and Phenol-Formaldehyde Binder Brucite Synthetic Chrysotile and Nickel Synthetic Chrysotile and Chromium Synthetic Chrysotile and Cobalt Amorphous MagnesiumSilicate and Nickel Amorphous Magnesium Silicate Synthetic Chrysotile and Manganese Talc with low metal content Talc with high metal content Brake Drum Oust Synthetic Chrysotile (J-M) Synthetic Chrysotile (J-M) and Nickel Fiberfrax Fiberfrax (Hammer milled) Black Lake Chrysotile (Hammer mill) TOTAL NUMBER RATS Oose mg No. of Rats Cumulative Mortality Percenta 8e 6 mo. 12 mo. 18 mo. 24 mo. 3 x 3. 5 3 x 3.5 3 x 3.5 3x15 15 15 15 15 3x15 15 3x15 15 3x15 15 3 x 3.5 15 3 x 3.5 15 3 x 3.5 15 4 x 3.5 15 3x15 10 3x15 3x15 10 10 1 x 25 3 x 25 10 10 7 7 13 20 7 7 7 80 13 27 40 30 40 20 50 10 73 100 73 93 93 53 73 100 33 47 53 27 40 80 60 73 93 27 40 100 87 87 67 80 67 47 47 40 -- 50 -- -- 20 -- -- 50 -- -- --_ 3x15 10 50 50 ---- 1 x 25 25 44 1 x 25 25 40 ---- 2x10 10 0 4 x 3.5 40 12 22 4 x 3.5 40 28 35 -- 3 x 3.5 40 55 58 72 _ 3 x 3. 5 40 52 70 85 -- 4 x 3.5 40 45 78 90 _ 485 . -r *> 02 018 1794 (Top row of figures signify number of anlm alel AO O Untrtfttod m Coaifol# 2 JAM#"* Ni Salt e Microquarts -- (Acid leached) Fiber Glass with hour.) 2 Bruclte Tiber O am m m O e 0 0 0 e o 0 u. 0 o O O o e e a b. O o e e e e a a a ifts 0 0 o 0 e 0 o 0 o u. e e O o 0 o e 0 o a o o u 2! Ground Bruelta 0 0 e O 0 O o o a 9 o e 0 0 -a -wasta.?1* , (Ball Muled. 2 beVtra) . Flb*r ** (Ball Milled. 2 hours) sf[^sa*GUM 0 0 0 0 *< 8 O 8 8 0 m m a 88 o a u Beoeficiated " Altapulgita x Oust from " Brahe Drum x Johnson's " Baby Talc 2 SUllmontte oo * " 0 d St e 0 e o 0o o d St St < m o a St m m d 2! Attapulglta # o CryietUi Ppt * VaifalAfi 2 Grace 7I6SB 2 Grace 716SC 8 St 8 8 m o e o o o d o e 8 O O 0 O o 0 o o 0 0 0 e o o o a 8 8 8 8 0 8 8 o 8 8 o e <n u PERCENT M ORTALITY-HAM STERS * S urvivor* Sacrificed e See A ttachsd Sheet 2 Grace 716JC (l-PR-75) ,, Fibrous Glnaa - tCAWi-.oo ,, Silicon Whiskers Heated and 3 Treated Ppt Washings Talcum -- Powoir Ground Fiber 2 Glass (an Gorskt) - teiftum 8 a St St ana 0d Mi m m Od #4 e O 0 9 0 o 0 d o 7 a m 8 8 8 d 8 m m m m d ? St m 8 O X < --CP e 8 8 0 m e1 PH 40)jv uviiodvi jo ivpvoyi INOUSTftlAL HYOICNS FOUNDATION OF AMKHICA. INC. Attachment to Table, Percent Mortality---Hamsters The following dosages were used: A = 3 x 3.5 mg B = 1 x 3. 5 mg C = 1 x 1.75 mg D = (two dosages were used) 12 = 1x3.5 mg 12 : 2 x 1.75 mg E = (two dosages were used) 4 = 3x3.5 mg 8 = 6x1.75 mg F = 2 x 12. 5 mg . -- *> 02 018 1796