Document akGK5B7JgGxNYEppJpEGOL3X
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST
TEST SUBSTANCE
Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, oras a component ofFC-3017. (1Octanesulfoniaccid)(CAS # 2795-39-3).
Remarks: The 3M producbon lotnumber was notnoted. The testsample isFC-3017. CurrentinformatioinndicatesFC-3017 isa mixtureof 0.67% PFOS, 20% diethyleneglycolbutylether,55.41% water,21% ethyleneglycol, 1.33% Hydroxy foamer, 1% sodium oetylsulfate0,.5% polyoxyethylene monooctylphenylether,0.04% sodium laurylsulfatea,nd 0.05% tolyltraizole.
The followingsummary applies to a mixture with incompletely characteilzedconcentrations of impurities.Data may not accurately reflecttoxicityof the tluorochemical component of the testsample.
METHOD
Method: OECD Guideline209 GLP: No YearCompleted: 1995 Analyticalmonitoring: Dissolvedoxygen concentrationsp,H and temperature. Statisticamlethods: Resultswere determined by calculatioonfthe % inhibitoninrespirabonrate. Test organism source: Activatedsludge mixed liquorcollectedfrom the Metro Wastewater Treatment Plant,St.Paul,MN. Test condition:
Dilutionwater: MilliporMeilli-Qrwmater Synthetic sewage: per OECD method Reference and testsolutionpreparation: A stocksolutionofthe referencesubstance,3,5-dichlorophenowla,s prepared by dissolving 500 mg in1OmL 1N NAOH, dilutedto 30mL withMilliporMeilli-QTm water,then broughtto the pointof incipienptrecipitatiownith 1N H2SO4 and dilutedto 1L withMilli-Qrwmater. The pH ofthe referencesolution was measured tobe 7.7. Test substance was added directltyothe testvessels. Test vessels: Not given. Number of concentrations: one plusblankcontrol,induplicate Temperature: 18.9- 20.6 OC Totalsuspended solidsand pH on day of testing:3.8 g/L(TSS in final1:.5g/L),initipaHl 6.4 and finalpH 7.1 Element Basis: Respirationinhibitioans determined by oxygen consumption. Remarks: Values correctedto20*C forcalculabons.
RESULTS
Nominal concentrations: 2 Blank controls,1000 mg/L plus 10 mg/L concentrationforreferencesubstance,allin duplicate.
Elementvalues:
30-minuteEC50=>1000mg/L 3-hour EC50 = > 1000 rng/L
Element values based on nominal concentrations.
Remarks: Tesfingwas conducted on themixtureas describedintheTest Substance Remarks field.The valuesreportedapplytothatmixtureand not the fluorochemicaplroportionalone.
CONCLUSIONS
The testsubstance 3-hour EC50 foractivatedsludge respiraboninhibitiownas determinedto be >1000 mg/L, the highestconcentrationtested.
Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133
DATA QUALITY
Reliability:Klimischranking= 2. This,study meets the criterifaorquality testing.However, the sample puritywas notproperlycharacterizedand the studylacksanalyficaclonfirmationofthe amount offluorochemicalproportion insolution.
REFERENCES
This studywas conducted by the3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number N3188,1995.
OTHER
Last changed: 6/26/00
ENVIRONMENTAL LABORATORY ACTIVATED SWDGE MIXED UOUOR
ILRIV3 /'Fy Date 9/.2!Z/f5 Analyst Page / of 7
Type Test @)Z
il/t
alcci
71f
ACTIVATEDSLUDGE MIXEDLIQUog
Date Obtained c?121?lrl
Date Used 9 2
-5-
Mixed Uquor Suspended Solids(MLSS)--3
g/L Adjusted to
g/L
MLSS In finaltestsolution
g/L
InitiaplH l@@ (Isttestsoln.prepared)
FinalpH 7 1 (lastsoln.prepared)
Comments ""CSL e c,c,0:c@ C,t'
I oic Cti-t-.Ik4r?@ IL f 4ttig.FIlVr.4(ey,r
L CL
I-aLf
TEST SUBSTANCE
Pample Description
InitiaplH of highestconc. tested
Adjusted to PH-.@..
Comments
13EFERENCE SUMANCE Sir@ple"'D@4,scriptior,
pH. of,stock solution
Lill6 r 4-115LiLeel@) mg_/L)
Adjusted to p
coN N
ura id
-..iperdWrd'Range.,--,@1,)1,
c
F@2.(7
ENVIRONMENTAL LABORATORY
LR-A/-Z
OECD ACTIVATED SLUDGE RESPIRATION INHIBITX)NTEST #209 TEST SOLUTIONS PREPARATION
Test Substance:
7
Sample Description:
ZA I
Analyst:
Date:
Test Conc. ffigadded
mgll
per 500 mL
mL MO(l) water mL syn.sewage
n-L microbial
R2r 500 mL
feed 2&r 500 mL inoculum2er 500 mL
TotalVolume mL
P7
-7
L R. ENVIRONMENTAL LABORATORY
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
RAW DATA
Test Substance:
3c, 7
Source of Activated Sludge Mixed Liquor:
Date Received:
Z
Analyst:
led,@fe lo-eo
Date Used:
30-MINUTE EXPOSURE
Test Substance Concentration(.4)
0 5'.. 1 0
DLQQOLVED OXYGEN CONCENTRATIONS, nxyl
TIME-MINUTES
Temp
1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0- 5.5 OC
SK CONTROL 1
/CC el r-)
7,-7@- 5 7. 7,'7. q c,e.,(rq, 6-
(11-61(0-13 r-I-1, 15.4 -clI- t1.-7 q -.,N. 5 Z- 14-7 '12-
5 13. ri2,C 126.G
BKCCgfrPXXU
1 .2-1
3-HOU R,EXPOSU
Test Substance Concentration
BKCCNTRMI
0-5. 1 0
DWMVED OXYGEN CON@@@
TIME - MIN UT ES 's 2.0',@, @.5 3.0 @.51
mgrl4.0 .4.
Dj' 5 5
7,0
-7;1--5-17@O (r,. co
aim
-i
L-WRONmeTrAL LABORATORY OECD ACTIVATED SLUDGE RESPIRA'NON CALCUATION Cf RESULTS
INHIBMON
TEST #209';@,'L,,@;,,@
Test Substance: FC-3017
Analyst:
30-Min toExposure
D-issolveOdxvci)nConcentrations.
ma/L 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0
BKI
7.7 7.5 7.3 7.1 6.8 6.6 6.4 6.0 5.7 5.4
iooo.o 6.6 6.3 5.9 5.4 5.1 4.7 4.3 3.9 3.5 3.0
Dw. 7.1 6.6 6.2 5.9 5.4 5.2 4.7 4.2 3.8 3.4
5.5, Tom.*c
5.2 20.2 2.6 1-120*6 3.0 20.3
.. . Ra
0.5285 0.5 0.8133 0.7 0.8073' 0.7
.BK2 7.7- 7.6 7.3 % diffincontrol(s4): 6.0
3-Hou Exposure m a/L 0.5 1.0 1.6
BKI 8.0 7.8 7.6 iooo.o 7.7 7.5 7.3 Dw. 8.0 7.8 7.7
7.0 6.7 C,4. 6.i- 6.9 ECSO (96% Cl):
5.f; f).3
20.0
DissolvedOxvcan Concentrat ns. 2.0 2.5 3.0 3.6 4.0 4.5 S.o
7.6 7.2 7.0 6.7 6.5, 6.3.- 6.0: 5.8,; -@@019.3 7.0 6.7 .6.5 6.2 6.8 -5.5.- 6.3 :-S.Ol@ 7.4 7.1 7.0 6.7 -6.4 6.2 5.9@ ,o6 7z. zy19.3
0.6479----10.6 'RaCn
.4568-,.0.'4 0.6697@ 0.6 :Oi4830' 0.6
1 *Okq
BK2 7.9 7.8 7.6 7.4 7.1 6.9 6.7 6.4 6.2 6.0 @S 7@, 18.9
% diffincontrol(s4): 4.9
ECSO (95% Cl):
(1)A.Oxygenuptakr*ol(ern0g2&-min) (2)Ra-R adimietdo2(rC-(Ple.Exl (3)% lnhiNtWlo(RaCfn)-ReSI(RACM)IIOO
sRoAC1M..072 nmn nom'sdP,
Ok- bW* oftCLa- $W"b,
(4)% differeimnecotnrob:ItRaSki-RaBk2VRaCm(lTiwOocOoraronlisusbtewknn16%d eachothw.) Tobs-5-om tmp Ti*$.WC
(5)Meanad)usiRo.dconvol(sR-aBkl+R&BIQY2
O.'46W.- 0.5 RaC
P,@
ENVIRNOMENTAL LABORATORY
OECD ACTIVATED SLUDGE RESPIRATION INHIBMN TEST SOLUTIONS, PREPARATION
LR A/ TEST$209
Test Substance: sample Description: Analyst: Date: Stock Solution Preparation:
C C%t e (*/%C
e
Ila.
-72
itifIUIC, @i?,C.
e-^)-a
04 e_
1 @414
A,'A@, C
v
Test Conc. mg/L
mL added per 500 mL
1C>
I C:>
ML MO(l) water mL syn.sewage
22r 500 mL
feed per 500 Div
-7q
mL mkmbial inoculumper 500 mL
TotW Volume mL
'ZA mI
LR
ENVIRONMENTAL
LABORATORY
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
RAW DATA
Test Substance: Source of Activated Sludge Mixed Liquor: Date Received: Analyst:
2!'
Date Used:
30-MINUTE EXPOSURE
DISSOLVED OXYGEN CONCENTRATICNS. nigl
Test Substance Concentration
TIME-MINUTES
Temp
L) 0 -5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 OC
BK CONTROL I
BK CONTROL 11
1-7 1-7
3-HOUR EXPOSURE
DISSOLVED OXYGEN CONCENTRATIONS, mgL
Test Substance
TIME - MINUTES
Temp
Concentration IL)0.5 1.0 1.5 2.0 2.5 13.0 3.5 4.0 4.5 5.0 5.5
OC
BK CONTROI- I
EK CONTROL 11
pH of activatesdludgemixed liquor: Initial pH of highestconcentrationtested: Initial Tt:sttemperature:
I/
e
Final Final I .
ENVIRONMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION INHIBMON CALCLLATION OF RESULTS
TEST #209
Test Substance: 3.5-DICHLOROPHENOL
Analyst:
30-Mln to Exposure
DissolvedOxygen Concentrati s. mq/L
mg/L 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5
BKI
7.7 7.5 7.3 7.1 6.8 6.6 6.4 6.0 5.7 5.4 5.2
10 8.0 7.8 7.6 7.5 7.3 7.1 - 6.9 6.7 . 6.6 6.3 6.1
Tomp, IC
20.2 20.1
L.R N318 Date: 9/2
R (1) Ra 0.5285 0.52 0.3745 0.37
BK2
7.7 1 7.5 7.3 7.0 6.7 6.4 6.2 5.9 5.6 5.3 5.1
""0diffin controls(4): 5.0
20.0
0.5479 10.54 Racm
3-Hou Exposure
Dissolved Oxygen Concentratins. mq/L
mg/L 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 Twnp-.C R (1) Ra
BK1 8.0 7.8 7.6 7.5 7.2 7.0 6.7 6.5 6.3 6.0 5.8 19.3 0.4558 0.47
10 8.2 8.1 8.0 8.0 7.9 7.8 7.7 7.6 7.6 7.5 7.4 20.0 0.1552 0.15
BK2 1 7.9 1 7.8 7.6 01. diffin controls(4): 4.9
7.4 7@l
6.9 6.7 6.4 6.2 6.0 5.7 1 18.9 0.4655 0.50 RaCm
tli R. Oxygen uptakerate(mg 02/L-min) @21Ra. R adjustetdo20'C- JR/o.Ex) ,31 % inhtbdioni.(RaCm).R&S/(RaCm)IIOU t4)% ddiofemeInoonirtasllR&Bkl.RaBk2itRaCmI(lTOwOocontrolmsustbe w"hin15% ofeachother) t5iMean adjusteRd. controlsI-R&Bkl.R&Bk2y2
1 072 R&Cm- mean ad@ntedR.controls @Tllokb.lankoorwirSo.l,sample
bs- sampletomp Ix--Tobs-20'C
..
Chain of Custody Record
3M Environmental Laboratory
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3M Fs"@ster
02298
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Item
2 3 4 5 67 8 9 10 11 12 13
Item
Sample Description
Date
7V@r 5
Time
30
PIatri
Number Containers L,
ofeach
- .l3r_lE
Preservatives
0 u
z0 zx
z
El Samples SplitSee At
Commenti
-- -
RelinquishedBy/Affiliation Time
v
Date
Shipped Via
Received By/Affiliation
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See Revers