Document aOvn6D9Qx64eoYG3ELpNwGe9
ACUTE TOXICITY TO FISH
TEST SUBSTANCE
Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, or as a component of FC-185F. (1Octanesulfonicacid)(CAS # 2795-39-3)
Remarks: The 3M productionlotnumber was 1-2303. The testsample is FC-185F, cc 817-19. Currentinformationindicatesitisa mixtureof4% PFOS, 36% diethyleneglycolbutylether,29.29% water,14% ethyleneglycol, 8% Sultonefoamer,8.35% sodium octylsulfate0,.3% sodium laurylsulfate,
and 0.06% tolyltriazole.
The following summary applies to a mixture with incompletely characterized concentrations of impurities.Data may not accurately reflectthe toxicityof the fluorochemkal component of the testsample.
METHOD:
Method: ASTM-E729-80
Type: Staticacute
GLP: No
Year completed: 1982
Species: Pimephalespromelas
Supplier:Dale FaftigFishFarm, Brady,NB
Analyticalmonitoring: Temperature,pH, and DO
Exposure period: 96-hours
Statisticamlethods: LC50 valuescalculateudsingboth moving average
angle and probitprograms inconjunctionwiththe 3M TRAC Computer
system
Test fishage: Juvenile
Length and weight:
Average length 38 mm
Average weight 0.38g (wet)
Loading: 0.48g fish/L
Pretreatment:None
Test conditions:
Dilutionwater: Carbon-filterweedllwater
Dilutionwater chemistry:
Hardness:
252 mg/L as CaC03
Alkalinity: 225 mg/L as CaC03
Lighting:A dailyphotopedod of16 hourslighatnd 8 hoursdarkwitha 30 minute transitiopneriodwas maintainedthroughoutthe testingpedod.
Stock and testsolutionpreparation: Test solutionswere createdby direct individuawleight additions. Concentrations dosing rate: Once
StabiliotfythetestchemicalsolutionsN:otnoted Exposure vessels: All-glasasquaria,35x2Ox25 cm containin1g6 literosf testsolution Number of replicates: 2 Number of fish per replicate:20 Number of concentrations: fiveplus a blank control Water chemistry during the study:
pH range (0-96 hours): 8.0 - 8.2 (controlexposure) 7.7- 8.2 (370 mg/L exposure)*
Temperature range (0-96hours): 17 -21 OC
Dissolved oxygen range (0-96hours): 5.8- 9.2 mg/L (controlexposure) 1.3- 9.3mg/L (370 mgll exposure)*
370 mg/L (second highestconcentration)exposure values used because the highestconcentrationused resultedintotalmortalityby 24 hours.
Remarks: The dissolved oxygen concentrationwas below 60% saturationin allexposure concentrationsexcept the controlby 48 hours. This may have contributedto the observed mortality.
RESULTS
Nominal concentrations: Bk control,100, 155, 240, 370, and 560 mg/L Element values: 96-hour LC50 = 344 (318 - 376) mg/L
Element values based on nominal concentrations
Remarks: Testing was conducted on a mixtureof as described in the Test Substance Remarks field.The values reportedapply to thatmixture and not the fluorochemicalcomponents alone.
CONCLUSIONS
The testsample 96-hour LC50 forfathead minnow was determined to be 344 mg/L with a 95% confidence intervalof 318 to 376 mg/L.
Submifter: 3M Company, Environmental Laboratory,P.O. Box 33331, St. Paul,Minnesota, 55133
DATA QUALITY
ReliabilityK:Jimischranking= 2. Testing meets the criterifaorqualitytesting. However, sample puritywas not properlycharacterizedand the study lacks analyticalconfirmationof the amount offluorochemicalproportioninthe solution.
REFERENCES Test was conducted by the 3M Company, EnvironmentalLaboratory,Lab Request number 7544,1982. OTHER Last changed: 6/26/00
I
orm
-11-C
TECHNICAL REPORT SUMMARY
D &to
2/l/82
.tO: TECHNICAL COMMUNICATIONS
CENTER - 201-2cN
(Important- Ifreportisprintedon both sidesof paper,send two copiesto TCC) See "GuidelinesforCompletion of TechnicalReport Summary" availablferom InformationLiaisonor TCC (3-5545).
Division
Dept Number
Environmental
Project
Laboratory
(EE & PC)
0535
Prol*ct Number
Colpmercial
Report Title
Chemicals
9972210100
Report Number
Aquatic Toxicity
Testing:
FC-185F
(cc8l7-19)
L 1-2303 LR 7544
007
To
E. A.
Aghro_
Reiner
A. N.*&Iter -
Notabo4ok Reference
2-3E
Employee Numberis)
09362
No. of P"es Including Coversh"t
SECURITY 10.
13 ooon
0 cloud
KEYWORDS:
IncludLeabCode
4OR R E(JeefefSJMRCEJITV E ne the acute
varying concentrations
3M CHEMICAL REGISTRY
Now ChwnicalRseported
0 Yes
Ki No
aquatic LC50, followin
ex3osure to
of FC-185F (CC817-19) Lt
-2303.
EE & PC-Div. (Env. Lab)
Toxicity (Aquatic)
REPORT ABSTRACT: Company R&D.
This abstractinfomwtion isdistributebdy the TechnicalCommunications Center to alert3M'ers to
The acute static aquatic 96-hour LC50 values obtained for
r,-ithc.acmlinnows (PimLph,-il(--sprom(--las) followinq L-xposure to
FC-185F in replicii-te-d experiments were 339 (not defined)
and 372 (333-415) mg/l. The combined.96-hour
LC50 was 354
(0) (not defined) mg/l as calculated using the probit
analysis statistic.
@Data generated limits
include LC50 values with 95% confidence
Information lnitialc
FC-185F/ANW Page 2 February 1, 1982
CONCLUSIONS
In these replicated 96-hour LC50 aquatic tests, the calculated values
for fathead minnows (Pimephales promelas) were 339 and 372 mg/l.
Materials possessing an LC50
this order of maqnitude are considered
to be practically nontoxic based on the NIOSH criteria for aquatic
toxicity interpretation. (2)
II]TRODUCTION
The test substance is a foamy, clear, dark amber liquid, soluble in water at ambient room temperature. Tests performed on this material evaluated its potential aquatic toxicity and the data generated form the basis of this report.
ACCLTMATION AND EXPERIMENTAL
DESIGtl
The test protocol utilized for this study was modeled after that
described by ASTM-E729-80.
(1)
The fathead minnows (Pimephales promelas) were obtained from a private hatchery (a). Stock E71-sh-wereheld in a flow through system fiberglass holding tank filled with carbon-filtered well-water at 14-16 degrees C. A daily photoperiod of 16 hours light and 8 hours dark, with a 30-minute transition period was maintained throuqhout the acclimation and testing period. The fish were fed Tetra-Min (b) daily, food being withheld 48 hours prior to and throughout the test period. Fish were acclimated to test temperature for 2 days prior to use in assays.
Twenty (20) fathead minnows, uniform in size, were tested at each concentration of chemical. All-qlass aquaria, 35x2Ox25 cm containing 16 liters of diluent plus toxicant comprised a study chamber. Test fish were randomly allocated to each test chamber within 30 minutes followinq toxicant addition. The fathead minnow experiments were conducted at 19+2 degrees C. Mortality, temperature, dissolved oxygen level, and pH of- all test solutions were measured at 24-hour intervals or until total mortality had occurred. General observations were recorded relative to swimminq pattern and behavioral chanqes induced by exposure to the test chemical.
The acute short-term static bioassays were performed on FC-185F using carbon-filtered well water of known composition and constant quality as the diluent (Tables li2).
a) Dale Fattig Fish Farm, Brady, Nebraska. b) Commercial Fish Food of known composition.
FC-185F/ANW Page 3 February 1, 1982
The 96-hour fish studies were performed in replicate usinq the
following FC-185F logarithmic concentrations:
100, 155, 240, 370, and
560 mq/1. This range of dilutions was selected to bracket 96-hr LC50
values predicted from preliminary exploratory toxicity tests.
LC50 values with 95% confidence moving average angle and probit TRAC Computer system.
limits were calculated usinq both programs in conjunction with the 3M
Monitorinq equipment used included ASTM thermometers, temperaturr=--compensating pH meter, a Yellow Sprinqs meter, and a Taylor temperature chart recorder.
Orion dissolved
oxygen
RESULTS
The fathead minnows (Pimephales promelas) used in these studies had an average weight of 0.4 grams and a---fe--ng-otf-f3f.8 cm. The loadinq factor was 0.48 g/l; the maximum recommended loading factor is 0.8 g/l (1). Temperature, pH, and dissolved oxygen values and ranges obtained during this study were: 19+ 20C, 7.7-8.2 pH units, and 96-hour dissolved oxygen values raniied from 1.3-5.9 mq/1. These latter values calculated out to a minimal oxyqen saturation of 14-63%.
In this replicated study, the fish 96-hour LC50 values were 339 and
372 mq/1.
Chemical possessinq an LC50 value in this ranqe are
considered to be slightly toxic to the aquatic environment (2).
The data reported herein are based on studies performed by 11. T. Elnabarawy and R. R. Robideau. Accompanying data sheets comprise original data. A copy of this report is filed in the Environmental Laboratory Archive System, Building 2-3E.
It should hf- noted that the qenerated results were reported in the absence of information relative to the "purity" and the "stability" of the chemical substance. The reporter] LC50 values were calculated on the basis of the "observed mortality rates" and of the calculated initial concentrations of FC-185F in test solutions.
REFERENCES
(1) ASTfi-Standard Practice E729-80. Standard Practice for Conductinq Acute Toxicity Tests with Fishes, Macroinvertebrates, and Amphibians. American Society for Testing and Materials, Philadelphia, PA 1980.
(2) Registry of Toxic Effects of Chemical Substances, 176 Edition, Christensen, H. E., and E. J. Fairchild, Eds., p. XV and Ci, U.S. Department of Health, Education and Wt-.1fare;National Institute for occupational Safety and Health, Washington, D.C. (June, 1976).
TABLE 1
WELL WATER QUALITY
SAMPLE DATE: 1/28/82 SAMPLE TAKEN: 3M Bldg. 2-3E, St. Paul
SAMPLE DATE 1/28/82 (TAKEN FROM ROOM 5)
PARAMETER
CONCENTRATIO17
ENV. LAS WATER QUALITY BOOK REF.
pll Residual Chlorine Ammonia Nitrogen Conductivity M-Alkalinity Total Solids Total Hardness Total Fluoride Dilute COD Total Coliform Standard Plate Count
7.7
8
<0.02 mg/l
9
<0.1 mg/l as N
8
330 umhos/cm at 250C
9
223 mg/l as CaC03
8
315 mg/l
8
252 mg/l as CaC03
8
<0.02 mg/l
9
<0.4 mg/l
9
Negative
10
21 per ml
10
Aluminum as Al*
0.2 mg/l
14
Arsenic as As*
<0.005 mg/l
14
Boron as B*
<0.2 mg/l
14
Cobalt as Co*
<0.02 mg/l
14
Copper as Cu*
<0.02 mg/l
14
Chromium as Cr*
<0.03 mg/l
14
Cadmium as Cd*
<0.01 mg/l
14
Iron as Fe*
<0.02 mg/l
14
Nickel as Ni*
<0.02 mg/l
14
Lead as Pb*
<0.05 mg/l
14
Silver as Ag*
<0.02 mg/l
14
Zinc as Zn*
0.05 mg/l
14
Mercury as fig*
<0.2 ug/l
14
PCBIS**
ND
95
Total Organochlorine**
ND
95
Pesticides
Total
ND
95
organophosphorus**
Pesticides
Sample Date 1/28/81 **S ample Date 3/4/81
TABLE 2
ENVIRONMENTAL BLDG.
ST. PAUL,
LABORATORY 2-3E MINNESOTA
FOURTH QUARTERLY REPORT, 1981 WELL WATER SUPPLY FOR AQUATIC MLOXICITY TESTING
PARAMETER
TOTAL HARDNESS* as mg/l CaC03
TOTAL "M" ALKALINITY* as mg/l CaC03
ELECTRICAL SPECIFIC CONDIJCTANCE* as umhos per cm2 at 250C
TOTAL SOLIDS* as ing/l
pii**
NOV 2 248 228 330
320 7.6
NOV 30 252
DEC 29 256
AVERAGE (+ S)
252 + 4
226
221
225 + 4
340
380
350 + 27
300
308
309 + 10
7.6
7.7
7.6 + 0.1
STANDARD PLATE COUNT*** per 1.0 ml
2
10
27
13 + 13
CRITERIA OF CONSTANT QUALITY
Monthly ranges should be less than 10% of their respective averages. (ASTM Standard E729-1980.)
Monthly range of pil should be less than 0.4 unit. E729-1980.)
(ASTM Standard
Total bacterial densities should not be greater than 500 organisms per ml. (USEPA-600/8-78-017, p. 101.)
ENVIRONMENTAL
LABORATORY
AQUATIC TOXICITY DATA SHEET
DEFINITIVE (FULL-SCALE TEST)
LR
De Tes--!RlQgVK. &UTF
SftrMaterial
Test edF r-j&sE-w-Bil--L9-1-ot
-st Organi-m-Fitnr=At-mi-mrcme-:&@.5oRAaMsou_MER.Date Recld 11/no JAL_Satch 1I
g.Wt.0, 5&granis Avg. Size_5LB_= No. Orgarusms per dose_,Zo Loading FactorA L4$;
g/l
.sh Disease TreatTient:_Ll-!DMC-
@st SolutionVolume 1(6_Liters/All-GlassTank Tank Dimensions: 35x2O an and 25 an deep
-me Started
:P" Date Started
Z_j Date Canpleted
-lutionWater Carbon-filteredwell water (Tenp.
-C., D.0.
ppn, pH
ivestigator.z@:@@C-@
LC 50 (9(5M%oCoUnKfidence Lim-its)
@ullSignature)PROIB@trAN*&.YsLs 1-"9
cy)s 0-,omi
D:N)
MOVItJi@ AVERAfiE ,ank InitialTotal
ANfiM Mtl"m 24 Hours
SWIL4 (2-Ctto48 Hours
wxg 7
@0. Conc. Amount Total No. pH D.O. Total No. pH ID.O. Total No. pH D.O. Total No. pH D.O.
mg/i Added
Dead
PPM Dead
ppm Dead
ppm Dead
pprn
Cont:rui
0
Ili
I
ckL n
0
0
0
-"u'q
10 -0
0
C)
0
1.4 2,
9 2-,1
ZLL
/a
0
0
A/
20
2-()
Test TenTp.OC
Initi.al&s Date
m@Tne@n :4k-liiftut
AALI
%Ll4/-2ZZ!2
Liqll%r),
ALIIF%LC- ILI 1,1
2condaryEffects (otherthan Mortality):
rank No. Symptans or Reactictis
Dntrol:
24-Hr.
4tT
-w-3;0"K
EC50
Total Number Affected
48-Hr.
72-Hr.
2^
f3-
as 63VAM fl
ki9--
LIIE
4
flu
2_1qo
arm5azA
96-Hr.
c--
ENVIRONMENTAL
LABORATORY
AQUATIC TOXICITY DATA SHEET
DEFINITIVE (FULL-SCALE TEST)
LR
pe Te-stginiAm,
Material Tested_EC-W?@ S_Fm
Atti-icIT-ot I- 4,W)m
@st organism_r_p_ggg&D__@@@Source
@ WES@:A Date Rec Id
rg.Wt. -0@grairs
Avg. Size % j,.g an l@b.organisms per dose_2_CLLoading
Batch
P2,)
84,46-1m)
Factor
g/l
i-shDisease Treatment: hlomjfast Solution VolLune_VD_Liters/All-Glass Tark
Tank Dimensions:
35x2O an and 25 an deep
-me Start ed_Jll_t@I)ate
Started. 1.]ZIDIS2-.,
1-lutionWater Carbon-filtered well water (Temp.
- - Date Ccrnpleted_4L VA&
C. I D.0...
pprn pH_I?.Z.)
ivestiga
---KA
LC (995%Z=
-ull Signature) -;R6iaA%NTALYSIS
mo\jl
ri A\VTE
ANC-T@
mErAm
SWC73.@-MOw-4-t
Lank
Initial Total
24 Hours
48 Hours
\@0. Conc. Amount Total No.
D.O.lTotal No. pH
mg/i Added
Dead
ppin Dead
1 ence Lin-Ltis)
CoR&uen
72 Hours -D.O.lTotal No. pH ppm Dead
i@ (kioUt-im
Lp@D0*4 96 Hours
D4.:mO. Total No. pH D.O.
ppm Dead
pprn
w@i
ax
Control
0
5,9
11)0 -VaQf)
I-s;:z
0
:-2zb
LM
CC))
riI c:>
-,c@ di,q P3
4/.-11@
Test Temp. OC
1.7
Initials & Date
cmTients:.L*=UmljlrAJkALI
'O)t@L4jALC-- im
AMRICtxr
-condary Effects (other than Mortality):
-rank No.
Symptans or Reactions
o,no4notro1l'
7
24-Hr.
@Jr+i
Nw e-
0 5 1/-2R
20 oe-"
ZaL-3. //@22
DAZE -
EC50
Total Number Affected
48-Hr.
72-Hr.
-AJLIAtg
AloAj r,@
157
2A' IT/Z=
in
96-Hr. lAbAE
4F
- ---
?V!:;T\ -::z7v0-@W
6
malp,
10
ENVIRONMENTAL
LABORATORY
AQUATIC TOXICITY DATA SHEET
DEFINITIVE (FULL-SCALE TEST)
TYPE TESTO@o&, MATERIAL TESTEDW--I-PSF TEST ORGANISM r4tmm
Mir-JNQIt)s
I-ZSOS
LC50's
(95% CONFIDENCE LIMITS) PROBIT ANALYSIS
T RUNS UN C,
UN
H TJRS
-AB--HQITRq
72 HOURS
96 HOURS
COMBINED
5SZ5 w--4Q 5 5 -,-w's sm :55clVKO
(Ko L4mils)(@k Lim,-M) 554
(,4-2-Ot-52-cl
@51ZW4 fl I
(Kio
96 L7LOURS
MOVING AVERAGE ANGLE METHOD
@Slfl@ut@s 24 11OUItS 48 HOURS
72 HOURS
96 IIOURS
.ui
'UN
,'4Sf ol
COMBINED 96 HOURS
ENVIRONMENTAL LABORATORY
AOUATIC TOXICITY DATA SHEET
EXPLORATORY (RANGE-FINDING TEST)
LR
Type Testlq6HR,_ la4C.UM :fsnkne_ Material Tested
05F (cc-1511l-q)LOtI-
Test Organ ismFA-rrr=M_ tiiMkWtl)Source
Date Recld.
Batch No.l::B7
Avg. Wt.
grams Avg. Size5,qCM No. Organisms p,-rdose - 5
Test Solution Volume 3 Liters/All-Glass Tank Tank Dimnsions:W IDE -MOUTH I GALLON GLASS J,
Time Started I]:
Date Started I 1q] B2j
Date Complete d
Dilution Water Carbon-filtered well water_(Temp.
)C., 1).0. A.c9
Investigator (Full Signature
-k L(: (95% Confidence Limits)
so
12-Y&Zj PPM, PH-Bi-)
Tank -!LO -
Cl_
LM
iA ZA
Init: Conc miz/1
Totalt Anoun Added
24 Hours
Total
D.O.
No. Dead pH PPM
Control
Ids
6 ....-...
-to --
10.0
48 Hours Total No.Dead pH
D.O. pps
72 Hours
Total H r.o.
No.Dead
ppm
la-,1 Lv@l
Hou@s Total No.Dead I )H
0 o
A/ 1, 7 0 09Li2
0 Test Temp. C.
zare-,
Zj Oe7
Date
Comments:
UMUI
MARK A&4EEg I-1011 f:)OLIIelcr- im ILVMEI@;
Effects other than Mortality
EC so
l@r 4w
:PRARY
(=A?-
Ewur 3RQQI,(IESfESM
Tank No.
Control
Symptoms or Reactions
4kgb
I-r
24-Hr.
AEl-AIE I
Total No. Affected
48 Hr.
72-Hr.
f @.0 "-Hr.
Ale
2A
-All),uIAFI-D
t'MMCItIS