Document aJrQkRErDjMa4gYa0YgLMwYB

AR 826 - O7T#3 ACUTE TOXICITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA) EST SUBSTANCE Identity: A mixture containingperfiuorooctanesulfonate, `which may also be referred to as PFOS, FC-95, or as a component of FC-203CE. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was 519. The test sample is FC- 203CE (2/21/89). Current information indicates it is a mixture of 1.84% PFOS, 30% diethylene glycol butyl foamer, 3.1% sodium octyl sulfate, 2et.h1e%r,p6o0ly.o2x3y%etwhaytleern,e2m.o5n6o%ocStuylltpohneenyl ether, 0.12% sodium lauryl sulfate, and 0.05% tolyltriazole. The following summary applies to a `mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect the toxicityof the fluorochemical component of the test sample. METHOD: Method: (OECD Method 202 and U.S. EPA Method EG-1 Type: Acute static renewal GLP: No Year completed: 1989 Species: Daphnia magna Supplier: In-house cultures. Initial brood stock from Aquatic Bio Systems, Inc, Fort Collins, CO. Analytical monitoring: Temperature, pH, and DO Exposure period: 48-hours Test organism age: <24 hours old Statistical method: Not given. Test conditions: Dilution water: Carbon-filtered well water Dilution water chemistry: Temperature: 20 C DO. 9.6 mglL pH: 8.0 LSitgohctkinagn:dNtoetstgsivoelnu.tion preparation: The test material was added directly 1o the dilution water on a weight/volume basis. ECoxnpcoesnutrreatvieosnsedloss:in2g50ramteL: PDyarielxy glass beakers containing 200 mL of test solution and a water depth of6 cm. -- Number of replicates: 2 `Numberof daphnids per replicate: 10 Number of concentrations: one plus a blankcontrol Water chemistry during the study: PH range (0-48 hours): 001091 8.0 - 8.2 (control exposure) TDeismspoelr7va.et9du-ro8ex.y1rga(en1ng0e0r0(a0nm-gg4e/8L(h0oe-ux4rp8so)sh:uorure2s)1):C 86..5899..66 mmgg/.L ((c1o0n0t0romlge/xLpeoxspuroes)ure RESULTS Nominal concentrations: Bk control and 1000 mg/L. Element values: 48-hour ECso= >1,000 mg/L Element values based on nominal concentrations Remarks: Substance TReestmianrgkwsafsielcdo.ndTuhcetevadlounesarmeipxotrutreed as described apply to that inthe Test mixture and not the fluorochemical proportion alone. `CONCLUSIONS The test substance 48-hour ECso was determined to be >1000 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY tReesltiianbgi.litHyo:weKvieirm,istchhersatnukdiyngla=ck2.s Testing meets information on ptuhreitcyroiftertihaeftoresqtuasluibtsytance and actual measurements of the amount of fiuorochemical proportion in the `solution. REFERENCES TSth.isPasutlu,dyMNw,asLacbonRdeuqcuteesdtbynutmhbee3rMG1C3o5m8p-a1n,y1,96E9n.vironmental Laboratory, OTHER Last changed: 6/26/00 001092 .. DAEPNHVNIIRAOTNOMXEINCTIATLYLDABAOTRAATSOHREYET ESE -- UA AERensfH LrOz E 2a /24/s 57 VTL TGEESTOVFESTSEESLT:O25R0mGlAPyNreIxhSgrlsHa.s0Ss1bd:eiankse2trat4sTE(SneTonSaOtLesU)T.IONNO.VOOURMGEA:NI2S0M0SmPlERWATTERR EDEPATHT:SM2cNanL TIME STARTED. LilSpat DATE STARTED_.S////BQ DATE OMPLETED_.S/A3/89 WDAILSUTTIRONRWCAATELR/CEaFribodno-fiwleFtlCle)wra0te5edrC(oTnefimdpe.n_ce2L0imiCt,s)D2 .0./_Z0 le 0 ng/0 ly e HElLls Se HE EE TEE Too [maria] Tora] ots | tatours __] Vessel] Conc.| Amount Total NoJ pH |D.O. [TotalNo. D.0. (fom =| o [|| o ladas] TL ooolz] 2Tooolzoo]l o || a || 1 | o lauluol o laws] 3 3 Cr A rrr Tr] I > 3 (r orem r | 2r 7 | TT 27 | z mn Dinseins pee [750 5/2/87|755 5/3/57 Coments:. Ludi dual eightsSyst soleil appacice; clear uellad ~6 ps T(e3s3tOmBeCtDho#d2o0l2og"yDacpohnnfioarmSsp.witAhcutthee"Ifmomlolboiwliinzgattiwoon Tteesstt parnodtoRceoplrso:duction Test. (2) US-EPA EG-1 "Daphnid Acute Toxicity Test". 001093