Document aJXdrMgXN0G557NrE41nZvyrN
MICROBIOS MICROTOX TOXICITY TEST
Aft 336-0113 -/?
TEST SUBSTANCE___________________________________________
Identity: Perfluorooctanesulfonate, Lithium salt; may also be referred to as PFOS Li salt, FC-94, or FC-94-X. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecafluoro-, lithium salt, CAS # 29457-72-5)
Remarks: The test sample is a mixture of the test substance in water (approximately 24.5% test substance and 75.5% water). No calculations were made to adjust for the actual concentration of the test substance in the test sample.
M E T H O D _______________________________________________
Method: Microbics Microtox "BASIC" Procedure Test type: Static acute GLP: No Year Completed: 1994 Species: Photobacterium phosphoreum Analytical monitoring: pH and osmotic condition Statistical methods: EC10 and EC50 values calculated by statistical linear regression program provided for Microtox Test organism source: Microbics, Reagent Lot # AM025. Test conditions
Dilution water: Millipore Milli-QTM water Stock and test solutions preparation: A primary stock solution was prepared in dilution water at 2000 mg/L. The pH of the stock solution was then adjusted from 5.9 to 7.0 using 0.02N NaOH and an osmotic adjustment was made using 200 mg NaCI dissolved in 10 mL of stock solution. Appearance of test solutions was noted as "clear and colorless". All test solutions were made by proportional dilution. Exposure vessels: 3 mL glass cuvettes. Number of replicates: 2 Number of concentrations: Four concentrations and controls Element basis: Percent light loss
RESULTS___________________________________________________
Nominal concentrations: 125, 250, 500,1000 mg/L Element value: 15-minute EC-io = 564 (367-866) mg/L
15-minute EC5o= >1000 mg/L 30-minute ECi0 = 487 (320-738) mg/L 30-minute EC50= >1000 mg/L
001542
Remarks: Values reported are for the test sample. No calculations were made to adjust for the concentration of the test substance in the test sample.
CONCLUSIONS The test sample containing 24.5% Perfluorooctanesulfonate, Lithium salt exhibited a 30-minute ECsofor Photobacterium phosphoreum of >1000 mg/L. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY_____________________________________________ R eliability: Klimisch ranking 2. This study, while well conducted, lacks analytical data for: determination of the test substance concentration in the test solutions, and determination of the sample purity. REFERENCES____________________________________ This study was conducted by the 3M Company, Environmental Laboratory, Lab Request number M1018,2/07/94. OTHER____________________________________________ Last changed: 5/2/00
001543
ENVIRONMENTAL LABORATORY MICROBICS MICROTOX TOXICITY TEST
LR M1018 JBD 2/7/94
SAMPLE: FC-94-X
Test Conc.
___ imsM__ _
PERCENT LIGHT LOSS OF P. phosporeum AFTER:
5-Mln. Expoaura
15-Mln. Expour>
30-Mln. Bmamr
125 250 500 1000
EC50, mg/L (95% C .
0 0 0 2 > 1000
_________ LI______ _ _
7 5 9 15 > 1000 (-)
8 7 10 18 > 1000 (-)
001544
ample Description F c -q ^ x
Environmental Lab -- W orksheet
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001543
O U or\v* \ MICRC'TOX DATA REPORT
V % VJ
FILE NAME: M1018.K30
Investigator
Samole Description: Microtox Testing
Procedure: BASIC
Initial Concentration 1000 ma/L
T e s t Time: 30. m i n u t e s
NUMBER
1 0 / IT
TEST DATE; '
T E S T TI ME : _____"
A p p r o v e d o y : _____________ .
o-f F C - 9 4 - X
Osmotic Adjustment: NaCI
Dilution Factor : 2
Concentration Units: ma/L
CONC
GAMMA
97.94/ 77.53
97.79/ 73.S6
39.54/ 73,12
4 96.83/ 74,15
5 96.54/ 73.54
90.53/ 70.23 7 93.75/ 64.43
3 91.26/ 64=43
C O N T R O L I T / 10
S I .26/ 96.69
RATIOS
0.3406
CORRECTION FACTOR = 0.3494
125.0000 125.0000 250.0000 250.0000
500.0000 500.0000 10000000 1000 0 0 0 0
83.78/ 97.62** 0 0 5 3 2 *
0- 0 7 2 7 6 0.09 493 0 .04017# 0.10924# 0.11509# 0.09495# 0.23597# 0.20221#
Y\vCSC\C|k LcV * AfVsO^iT
iO ZDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD?
35 LO PE = 0.5607
` -_________________3
3 ______________________ 3
y ________3
3
-j 3 i
`T T T T T T T T ? 1
zzzzzzzz .
< w 3 *7
3 *7
3 -
3
38
3
3
EC50
`*
3 --Jy>
.10 @DDDDDDDDDDDDDDDDDDD'"DDDDDDDDDDDDDD.DDDDDDDDD ^DDDDDDDDDDDDDDDDDDDY
100 1000 CONCENTRATION- 10E4
10ES
C 5 0 3 0 4 8 .3767 (957. 4FIDENCE RANGE: 6 0 0 . 9 3 8 6 TO 7 1 5 4 6 2 , 1 3 9 5 )
iEST COPY AVAILABLE
# Us e d -For c a l c u l a t i o n s
001546
50 .
STATISTICAL REPORT FOR DATA FILE M1018.K30
E S T I M A T I N G EQ U A T I O N : L OG C = 0.3351 :< L OG b +3.4541
NUMBER OF DATA PAIRS USED: 6
RESIDUAL VARIANCE: 0.0254S
957. C O N F I D E N C E FACTOR: 5 . 0 7 2 2 7 FOR EC50
CORRELATION COEFFICIENT: R = 0.34731
COEFFICIENT OF DETERMINATION: RA2 = 0,71579
E F F E C T I V E C O N C E N T R A T I O N S <957. C O N F I D E N C E RANGE):
EC50 = 3 0 4 8 . 3 7 6 7 .na/L
EC20 = 957.7798 mg/L . E C 10= 4 8 6 .5695 mg/L
ECO! = 65.6808 mg/L
< 600.9886 TO 715462.1895)**#
< 474.9458 TO 1931.4672) < 320.5334 TO 735.4969) < 10.7405 TQ 401.6549)*#*
*** EXTRAPOLATED RESULTS
' IIVJVO
001547
"3 0 oo-o, ,
" V Q
INITIAL DATA
M1013.K30
tHIMH!1M/'/1MMMHMM1MMMMM1MMMHMM1HMMMMMMMMMMMM!111M11MMMMMMMMMM11M11M1MMM11MMMMMil M1//M/.rtftfpi
MBER
CONCENTRATION
GAMMA
% EFFECT
1
125.0000
0.073
**T.
125.0000
0.095
3
250,0000
0.040
4
250.0000
0,109
er
500.0000
0.115
500 -0000
0.095
/
1000.0000
0,236
c
1000.0000
0.202
6.78 S 67 3.86 9.85
10,32 8.67
19.09 16.82
7.73 6 ,,86 9.50 17.96
!1MI1MMMMMMMMMMMMMi1MMI1MMMMMMMMMMMMMMMI1MMMMMMMMMMMMI1MMMl1MMMMMMl1MMMMMMI1MmiMMMMMMMMM
C e r e T o u c h S P A C E BAR t o - c o n t i n u e ....
CCtCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
001548
SJ \ MICROTGX DATA REPORT
" iu iO
FILE NAME: N 1018.K 15
Inves-i-iaator : ^3"X5>"X>
S s m o l e D e s c r i o t i a n s Mi>.:rotox Tes ting
Procedure s BASIC
Initial Concentration : 1000 ma/L
Test Times 10 minutes
NUMBER
1 0 / IT
TEST DATE:
TEST TIME:
A o Droved bv;
of FC-94-X
Osmotic Adjustment: N&Ci
Dilution Factor : 2
C o n e sntration Units: ma/.L
CONC.
GAMMA
1 97.94/ 76.80
2 97.79/ 75.09
3 9.54/ 72.73
4 96.33/ 74.33
5 96.54/ 73,35
6 90.53/ 6?. 03
7 93.75/ 65.86
8 91.26/ 65.14
CONTROL IT/IO = 80.46/ 96.69
RA !IOS --
0 <p r*7'
C O R R E C T I O N F A C T O R = 0. 8351 .
125.0000 125.0000 250.0000 250.0000 500.0000 500.0000 1000.0000 1000 T000)0
8 1,82/ 97.62* 0.8381##
0.06503 rn VCCCCX
0.02817#
<J.0S72z# 0.09918#
0.09526# 0.18881# 0.17002#
<) .crV *
"/'Ceto
ri T o
10
M A
zdddddddddddddddudddddddbdddddddddddduddddddddddddddddddddd?
3SL0RE = 0.9269
3
J 3
33
3
3
33
3
33
j
3
3
33 3T
33
3
3
__! 8
EC50
3
3 4 6 __ _
3
. 10 QDDDDDDDDDDDDDDDDDDD''DDDDDDDDDDUDDDDDD''-DDVDDDDDDDDDDDDDDDY
100
1000 CONCENTRATION 10E4
10E5
503096.745
ANGEs 02.4584 TO 7.15917.8008) # Used for calculatrices
001549
/"S" o r \ v O
STATISTICAL REPORT FOR DATA FILE M1018.K15
ESTIMATING EQUATION: LOG C = 0.7754 k LOG b +3.4909
NUMBER OF DATA PAIRS USED; 6
RESIDUAL VARIANCE: 0.02548
957. C O N F I D E N C E FACTOR: 5 . 1 4 0 1 3 F O R EC50
CORRELATION COEFFICIENT: R = 0.04730
C O E F F I C I E N T O F D E T E R M I N A T I O N : R'v2 = 0 . 7 1 8 7 6
E F F E C T I V E C O N C E N T R A T I O N S (957. C O N F I D E N C E RANGE):
E C 30 = 3 0 9 6 . 7 4 2 2 inq/L EC20 = 1 0 5 6 . 9 3 7 3 rng/L E C 10 = 563.5806 mq/L EC01 = S7.7366 mq/L
( 602.4584 TO 715917.8003)*** ( 433.4232 TO 2237.1653)*** ' /\ 3 6 6 . 3 5 2 2 TO S 6 5 .3 U & 7 ) < 13.3214 TO 420.6264)***
*** EXTRAPOLATED RESUL TS
001550
t s r r \ v y> I
r r MIS
INITIAL DATA
mois,
MMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMMM
NUMBER
CONCENTRATION
GAMMA
V, E F F E C T
1
125.0000
0 0 6 5
Oil
125.0000
o .o s a
r*s
250.OoO
0.028
4
250.0000
0.087
5
500,0000
0.099
6
500.0000
0.095
7
.1000.0000
0,189
a
1000.0000
0.170
o .i1 S .0 6 2.74 3.02 '9.02 8.70 15.88 1 T * S 3
7. OS 5.33 3.36 15,21
H//MM11MMt`i il MMMMMSIMMMM11MMMMMMMMMMMMMMMMMMSIil MMHM '1MMMWMMMHNil MMMMilH111-IM MM1111Mi-tflMHMHMMM
ffiTTauch SPACE BAR to continue,...
001551
vj n \ M i ,
MICRGTCX DATA REPORT
""U i
FILE NAME: M1018.K5
Investigator r r Kc>
Samle Descrioticn: Microtox Testing
Procedure: BASIC
Initial Concentration : 1000 mq/L
Test Time: 5 minutes
NUMBER
10/ IT
TEST DATE
TEST TIME
Approved by
of FC-94-X
Os mo tic Ad jus tmen t : N a C 1
Dilution Factor : 2
C o n c e n t r a t i o n Units: rna/L
CONC.
GAMMA
4 X
97.94/ 8 5 .74
97.79/ 83.68
89,54/ 8 0 .52
4 9 6 .83/ 8 2 . 6 0
3 9 6 .54/ 34,41
f Q
90.53/ 3 0 .48
7 9 3 .75/ 77 91
3 91.26/ 73.10
C O N T R O L IT/IO = 52,-95/ 9 6 . 6 9
RATIOS =
0 ,3579
CORRECTION FACTOR = 0.8587
1 2 5 .00 0 0 125.0000
2 5 0 .0000 2 5 0 .0000 5 0 0 .0000 5 0 0 .0000 1000,0000
1 0 0 0 .0 0 0 0 83.91/ 97,62$#
0.8596$#
--0.01908$
0 .00352$ --0 .04508#
0 .00667$ " 0 * i7-j>$ - 0 .03404$
0.03332 0 .00342$
r.
Lc.V * A r t C S
,,.N0 TOXICITY AT CONCENTRATIONS TESTED $ Invalic data or controls
001552
\- s. ' :n - tiaL D A T A
NUMB:ER
5 " K'VV VCN .
CONCENTRATION
GAMMA
U
M1013.K5 w w M vm m m m iiM H tim im m im
% EFFECT
i 2
4 5 6 7 8 it 7
125.0000 125.0000 250.0000 - 250.0000 500.0000 500.0000 1000.0000
1000.0000 INVALID DATA REMOVED
-0.019 * 0 .004 t
-0.045 * 0.007 %
- 0 .0 is % -0.034 *
0.033
0.003 * FOR CALCULATION
-1.95 0.35
-4.77 0 .6 6
-1.82 -3 52
3.22 0.34
-0.80 -2.03 -2.67
1.78
.'T T o u c h S P A C E B A R to c o n t i n u e , . . .
CCCCCCCC. CCCCCCCCCCCCECCCCCC'CCCuCC
001553
