Document aJXZKbO5EZRn8eyR5Vx3bX8ya
3M REPORT: BIOACCUMULATION STUDIES
3M REPORT: BIOACCUMULATION STUDIF.S
March 8, 1983
March 8, 1993
66.
RR(e1ept2~o0or2rt8t7NN9o)o..*F00u00s11or((o55ct1h22e22m/7i79c9)a)ls""BBiiniooaaTcceccnuunmemusulslaeatetiioRoninveoorffFFFillsuuhoo"rroocchheemmiicceallss
in
in
Tenn.
Tenn.
River
River
Fish
Fish"
and
and
Report
Re~ort
No.
No.
100
100
(12128/79) "Fluorochemicals in Tennessee River Fish."
verityTThhoifisscppoaanitirenootffs,ppaaappneedrrssitishseqquGuiiiftteficccoolntnfyfuusosifinnagg,c, lulaa]rrggeedlleyytebbremceianuacsteioooafnfsi.iunnccooWsrrhrreeeeccntt sstttahanenddsaarraddrsse,, cccooomnnfbfuuissneeedddiedwneitntihtittayy aooffclkaabboeefllsclaaensaddt vsspaearmrmoipbtpylllieionnmfgg. cddoTeenhsstieieggsnnnetsiip,nnaaprrneeedrllasattthiimoeoannkdttiefoofiacoopuuutleftffyxaaclleolllflaaeannncddttussaaaalmmmcpppllellitienenrggmoppfinooiahinntotitowssu,,sa.tthhleeiWtlrrheeesesukunlnlttothwa3eld4seddedgllaieirttetlceecaont1mo0bbeooiuundrreadnuugnwnedrdietoehrurssstal.aannladdciiknnggofooffcletthaheer
problem. These papers make an excellent example of how a little knowledge can b~ dangerous.
6 -
Exhibit 2596.
22559966..00000011
3M_MNO1639837
3M MN01639837
Aom n
Teckdi--n--_dk heron summary
To. TECHMEAL CoMAmCATION CONTA - 31~26E8P~)RT S!~MARY
-- [Fram
(import = If rtis priviedan 30 ie ofpepe nd 0coo1s0 TCC)
DEC 20187
TT avirosmestat tortor a5 830) ous Environmental Laboratory (BE PC)
~" ~
0222
Decatur, Alabims = Tessesses iver rin See Dec&tur~ Alabxm& - Tennessee River Fish
Pq~ame~ ! Title
Bioscemulation of Fiusrochestcals ta Tass. iver Fish|~Ooor Btoaccumulatoa of Fluorochemtcala tn Tenn. River Ftsb
O1
To
bt Bacon : : D. L. B&coa
staan ._cagon Siam Jam i~. Gtl~oo
R= ES ebfIisEo emTares [SDTR[aemTtCoaetvmcuiuorannsetspeamaRraeieq,vruesra2.l0it As1ontbaaivlteydi.L sve.eahafoeaodrbtosqdlrueeasianntWniiistoeavitsoiarvdTehDbduaoaecreatrn"aieIa'ststLion of menrrd
BO
comentLab Reems Request | HERD
Fee
cu~mwre~crrv~: Qutlttative and qu~ttattve do~e~lnat~on o
F63~.
~-3925, ~d ~-3422 In ~l~h t~en fr~ the
Tennessee ~ver sbov~ and below Wheeler
~ca~ur plant. ~alye ~or org~ic ~d tnorganc
1uorLde in the ~
TE tEaotL~rmltsC~s)ettaattSheeacmtErxttarKshRtrssysooSeffn)a CC ahr~ aeorl4 rvccaatetfefisIhocsr((i,tlsasltaTulruuE~s TpFunticttuatse)),,
annulirl~) ~re ~lyz~ by gas chr~t~r~.
cc. 9. micker-200-3 cc : D,ItLcker-33~-3 Site A. We lter Aisa A. il4ode I zm)
2255996..00000022
a3M uoressess MN01639838
Teon. River Fisn/JEc
-
Tenn. River FIsh/JEG
_~_
5/22/70
5/22/79
1TaoOuCTION
oofIItrrtlgguaaoiinsnsriiocckkcahnoefTowmll~siuucooatrtrhhliiaaddsteet ca3IllM nee'vvs'eebsllissD Do,,eweccca11act00tuu..umr95rl,,apptppAAe~allaabb((ia1aInm3)m0(saf22i))psp:.hllaannitotIItt eeafhfMffal~lslauubeeaoannlrltstasootohhrbabayeseseennhehiaiggvhshhhioo-wwan (hat
5rrtflo1e0ouauaomag8wrateeesoeentchtha1idfe((33md)ff)i(eli(c44tssa)hbe)l.c.stccaaacbau"W uWnlggiihehtttthbh ilottieiahnhnvceeecslsttueeshhm eeccouooTfTl~meeabnbnftinseilnneeaeesscinddssieeoescfffaihasecRcRhtmiitooivvrceerinsarrs,l,snnaeetthrahlaserebonttnhhereeexaxtttoDDreessytccteaaepetptuunrrwwvaair-s
plant had detect~ble levels of flucrochemicals.
RREESSUULLTTSS AANNDD DpIrSaCcUuSsSsIrOoNn
eT oBTfhaacbibclihlheeathh1e1aarvvellieesists~ttthsah(eeT'4ttshsh3aeea0mm3ee,cco|onrrnececettTeeneHnnts-trt3triai9aoot3t~3niio,onntt,i,aimnmedeiinaaaTspspHpp-m m3tt,h4h, e3e2iin)otthhrrffeeiiseeshhfflooiuffuoorccrooooc~mchhppeeoom muuinnicddcassallss
of interest (F-6309,
1~-3925, and 1~-342~).
S3A gSGAuaarnrnm ndoaeapllaplliyftylaeeeeaetrir.ls"s33aee;,ooxxftIfteetsshhnnttohotchtww eaesn'siinnrreeatthshtlsthauhasuteelotlttsstbtgfhohaaeefsfsosotterfrrFrlsoolusittiiohhnn oreecrttoehoecsaddcshttihitiseisonnsm entesahsiiicecclcttiaemeltLldrudssraasccccbbctlhihti,oea,sasccnneorrnleeneanelpcpylceerreocnrconatddattivuutrfaacfaciisttttsseeiihhvvf,e,ettooousssydya&ssttseeem mr.,
atFo0onsdb bu.ilifto oaaaat.iccacugumrI~ eitellaacwtt~eiothatthlh~eeeoar~hlbhriereeesreseerffnlelupuooothrrrtaoosctchhet(~~ m3eii0ccam a)ll.~ ss cooleff
isterest. layer w~
interest.
Thess found not ~ese
W hrttGwehrehoiosennnuw~lhctcthsowoooliml~ceep~aeaFrrffleiitinsenhshggehwsstftihaahlthem meupoplrettleoooeasctstr.haa,lleilemtthihrffceleluauroloelJrrau~oorcrccrgoahthnee~ eetrrmm esncc(ilth~ hcc,aaaa)ldnl(n43ne.cc)el7.ool4nntccteaepasinpttffmtliaev((hTneT.FFC~ce)1o)o.sn1tfft3~oooirirnnpeerttdhdeh.eem moorree
SSteefh ixoixnnrp~pccleeicar~hnabbaawtoatoiitnictohheoenlntfThifcfiseoosahrhtrffiww l~tusahhehro irl:esse~hm acceaaaTauyuym lgguhbblhocteetraorrliihnenee]lcaaaotti~hthnceeeetaddels8 nsattto~ ,boed~itthoAha,7resere4ecscahh .ui,plmg~ ~ihaalap rvFpt~eas~ea.srlsoi~ s1intni.li1aaoe3bbFnsllaesp ttcc~yo~.offliisicecisi~eun .att~s
4fF3oldruoGcrohic~nhnoeenlimcoarctsastffisusoihut.yld tbiFev.leueuoxorp~eihcset~DoIarc:ogwlcsal blinoa(chce~llaar~geer in[lfuamttymorteissue,
fluor~hemical~ would ~ expecled.
CctFtfhh-ii4o6aoo3ra3nnn0n0n3~tnohrreleaal ttecochiissuasiatgtfhphfphiieararssrvveeh heses,,eecnnonstonwsottacatm meaatbpnbtpultoloooerhhnna~it3gSgiA Adhdh,oeoo,enrttrvsetthrrchcm aiac(cn.niaoiananccoeeooedtdnbtnhhet~ereffrrooaorartfrtifssiooeaF~ Fnnrm-0p 6seps3)lld0ee3ii9n.sn .
tthheeSiddsiiceseasSeeiccetteseddccumulaas explana ions Since bioaccumula-
no explanations
for the higher concentrations can be offered.
STTrtErhfhhiihaeaggelooftrrtt1,owotutohouuhoeessrlloffyyiCccisohsohhreccreeaooismmsacsppsam aaaamam lprrmpnleelppeddell8we(esttsw oWohSh~ ohaiwlwiyychehchhoollnGfeeoohhi~tasahddffLLlicacssvaovhhaoverlereuesssaesarrmsemeppptptl~lmarre.eeaxestseseys:.enssnbnteZffirTTraolhhhot~ieemiivvfeenrtrtheeeherccaaemossmoosoonncnrw wcoieinlffLlnlcoooltrrrvraemnatroeott)tihhtio.iicnssbbeosse ilss
S3otihfineovtfoerlueCcwoouorrlr~oetshv~caiamcnpaiiblsse w(reeihrgooorleoLuuskfliosynhc[ovrmoaapluareteshde~sFppbresihais~ihcerwoe~s l~ Toecri)li.on,
the results c~ be rigorously c~ared.
DTTFfahih1snee.hnw w"hTvThIia~CitCmteeplo0ebbfa~s002ss4:s.4,400ffrhroppiompdmpm,bb,&eellwwwoohhw hwioillleeeW~hhettfehehieeelsleherw wrhThiDDFiatCatsm ee.o, fccserra~0aa.papm0pppp0liil4eeee 11pffr3Brao,i,m :ehhaaamddFbooivvaaneewwW~ hwhhoehoeleelesneelleerr
GowoD~Tltffma~m atmnrriaiggRh,eliouiirlrrsggh`ahs~pTteeTtpoarrRa~sclCtseiiffisnlIllbctsu2luuiiAeooccddr,raauuoe~lonlcxhhhpaedeisoowmasnaa~lilmlaap ccypyaalaltweelliLthnsoooo.,nccls~ootLnn~hIiceecttffeeiosnnw wrVwhtthhoorrllutuaatilT hltteedde~ iioobbb Dhnneeaoi.,v~ sfen4de0dd1iro.ffl~L0wfiifn0invTvics4sRiiuu tCtnniigp grltetC~ eaoIi~s.nnuitnoldftt~l@ hsar~aebaem uiyeynthpprrttitthohnuhh~eeaesdauteecthcnppnclitth,enshnaeott..
OFto~leirovpiongssible egpl~a~ions for the higher T~ could be ~e
following:
22559966..00000033
3M_MNO1639830
3M MN01639839
Teor. River Fish/JEG
"i
Tenn. Slyer FIsh/JEG
sjzzym
Ta0E 1 FLUOK`IONCTIEENNIRCSASLEECONRCIVEENRTRAFTIISOHN (3pa)
sSaamnppllee
v-463~0o99
1A- Whole fier
0.40
0.40
18 ~I~
-
-
Core ~hole (3)~ish
0.2
21B
-
-
Ccoorree
((35))
0.82
0.08
a2A -- cae Core (5)
0.0~
1.48
3A - Gills
34 - Liver
207 1.46
SA - Liver
2.17
3 - bare (D131.333
30 = Muscle SA - Parte (7)
xD.
3A - Muscle
M.D,
n= Fat (
1.88 13.~5
3A
30
-
-
Pat
Gall
(8b)ladder
1.57
water blask 3A - Gall bladder nD.
~&ter bl~
EtBhlyalkacetate Ethyl acetate
xo.N.D.
N.D.
b1~ak
FTnu--e3390n22S5 8(1)
15-3422 (I)
0.
0.73
EEN
3.31
x0. (8)
N.D. (6)
0.80
0.60
0.38
0.38
0.43
0.43
0.
~.D.
62
~.12
0.74 nb.
N.D.
x0.
N.D.
TT ooettaanll ~ rCiooam~ npinn~cdl
ope) 1~ 1~
(~)
(3Fish
(2)
11
I ,13
00..4400
(1)
(~)
0o..o0o044 ((44))
2.74 (9)
2.74 (9)
.
Footnotes te Table 1
F((((1123o))))otFunPIDSh3Uuaso1oe-wmt-ded3ep3;s0d;l93e25t5ttoohhnteeo~aTrrarnfeeadd~rf,foboo1PzlrcrNe53een--.,31,364:4v122ea22lcghcc,accootama~bnn1n ino4onfoetect~doibbnneevvetaaarlrilfeuueinseesseohod.lltivvssseekddirr~se,vWppiloo~trrfhh~tieeldGeGd.tCC.,
parameters
p&rm~ers
reproductive
(2)
(3)
(4)
(4)
(5)
~ S o r~ TFeaer~es spalpud ~ rm~ mlesessss .,oscncnot~ tathanfrhrectaao,titzvppee3aa~tnrr,hh6~teeo18w sf coe ~ ootofnfhin,ce~cekkeniStrdtndoderntfsna~ecteiy~ loyt,,onioefotnarrn8o ifsetnlchcsetoeothdubb ~s.tft,,asisikslnh~ n ian.ea~ dd,d Sunple core, 3.81 ca 14 contatnod filei,
~ df1iaalcc ekt~ tbh,oeo aec..ore are Ivnortthcebracco,re kin,
representa~tve of ~he ~ot of the fish.
(5)
((((~678))))
SiK;NGCnDo~ a,ndD.nLpu.dilb ioai-=vtNtleescN aNa.oodotrteoo,fdtot3mGeu,u6csi1tctolrde~ o.;iawltdkeinact,ioohnbtllaolonCdr~.ierb~,olslcer.ti.pwrvao~ddurtcccatbrirtvaie~ls.sgrsa.iem.
(7) CuntS.ted of muscle, ~ktn, bl~, b~e. ~d
(8)
(9)
B ~ pBTaardsg8oea~dsno0n8vettihhneet,aacctAtuunaadllwww eeieigigh~ht$t boeofrfces sna~ tmpllooef
~uesaeecddh,, p1 1ar88t...8~%
less
less
thas
frown vel~:, and ~elEbt ke~ea~ of e~h part.
22559966..00000044
3M_MNO1639840
3M MN01639840
=|
TT 3M CONFIDENTIAL
Tenn. Hlver Fish/JEG
-4-
5!22/79
L~aler river residence time, older
Loaler location residence time. Different species a) Different feeding and life styles
b) Contains lsr~er wetsht percent of
which tend to bloaccumulate fluor~chemlcal~
the
) Larger fluorocbealcsl partition coefficients o~e 8~loe ~re representative of whole fish concentrations
{BuehiattsephemiHn,fooe TJoprseesgaesits]sSof siale tria csom,es fluor~itcals to a Ireater o~tont th~ either rhine b~s or
Tire 3 eis a resi ofeae h grote (1i ) svt spec Table 2 |Iv~e ~e results of the orlanI (RF) and inorganic
fluoride (F') co~,contratlon, in ppn, In the fish eaa~loe.
RnoT us 2 0 T ORGANIC (RF) AND INORGANIC ()
Sample
IA
9.7
24.8
2A
18.2
13.3
1D
10.5
later
N.I.
O.01
Joe ~oliele point~ out that th, high Inorganic ~l~ori~e walu~
mater Sprig, hy SySotaeTN Te Prewlo~lF ~aly~d, few a different r~tor, ~ she ~o ~ TR iLOH,e i a seMedRdenSi EiR AT gHdE ~o calculate 1~ levels of or~tc fluoride. CFdEomInpEaSriisSoAnNoffthha sgrmucn,ices3e maJrgeimn ffhuarrisd ccoonnetntent seonm b~lber, ~l~em tbu f~ t~ s~le t~ ~1~ the ~. ~ m S Ssumicsn eroriteaiini,tfKnhe sEell tle ST l hs ualyols of Te~ool~ ~er water ob~ hl~ orl~lo tl~r~ niy EaRE Eelreet fae ~ncentratl~s u~t~ f~ t~8 ~l~t. kt t~at tim, It w~
22559966..00000055
3M MN01639841
< Leve Welsh
fi
= [leo
Tenn. miver Fion/3%G --
Tenn. River Fish/JEG
saz 5/22/79
miLttaywwoaou~ ulluddnssleloy eemmoboeetcooe. leeiisnenmddSiiEcbceeaalttAoeerwatthVW hahaatehtteecettthleheaerer sDcsSIooeRmmsnTsh.ceeesnntLTtrFrhasaoitsttiioooasmnnaayyooovfehhfreef{lSccluahuohueorisroeuocadcbmh,~ebbm syy(1it)cc.a~ll~s
role ofDI volatilization of the fluorochemical when EoinE over the dam (1),
set~linl of fluorochemicals before the dam.
CCoompL arisonTof orgaynoic efilpuioroiyde hav.aluBeisfhofsrcomoTragbulaeiss 1flusoardid2e.shvoawlue Comparison of orEanlc fluoride values from Tables 1 and 2 show 38 EE Rampe TanhHE,A nSae hShoameaxFpricd.a 5.0l0o4s pLomastaor, thheere no correlation. For example, the hlShest or|anic fluoride value,
16.2 ppm for s~mple 2A, had the lowest
a een er una Sh rory fluorochemicals ~nalyzed. A possible ETeEne an aratens caiivabaieiuaelatityl.in, ~l'e or|8~lc fluorides Dissent in very
TFC, 0.004 ppm. for the
Bieh concencratione Thich expl~"ation is that there Tbhied rThpeecilooewewshticohreIhaaai.c the hiEh concentrations which
were not ~nalyzed for individually. hi.eat fs~ content, channel catfish,
Hi tht ton fluoride concentrations.
The h~d
species which had the the lowes~ orEanlc
WTitihm1einditedmsenmlplee poPpuolastieon ((31 fiRshsof ctuelee spTeecidsesansasdaycoe i~h limited s-sT.le population (2 fish of one species and one ot Cn Suatoams TThhea"bapleSaarrinTiotsblcoosnccelvusmiioent:e 1ba hFaitver of each of two other nDecies). It is difficult to drs~ any
Bn SemonSS meaningful cuuclusions. The onl~ definite conclusion In tba~
Emm
E1X.PERsIamMpElNTeALmaserinie
1.
ne3an~le materials 1h - SFmarl) cr hanneE l cactiR sn (Tt otairus
punctatus)
Cush
15 - WaHbhioAtveeAgbaR hneeele(ErHoDso~us chiyscpal, in ?ennesseecaught River. below Vhseler
20 - DWahmiteincTR raepnpnieEess(ePeamRsisviesr.anmularia), aught sbove Whecior
Sh = DLt~aamrrssin SSThheanmenene)sseSceuatRTUlvshe,Jn'.(Ferbateurustepereiatas), CaughS
sustaarbeonve ~beele~ Dms Ln ~enne~ee ILtvo~.
TEern
13m, yasas, mpoem astatndards7of
waa esr 70403.om VVUi-a2n8d2a5r.d,
s31da
PwAhCt3]I2dcwaeturtoe.prep0armeadrk
SL IR TR a A
3. Anslrsie tstrusmncsuteriale
Siesder: vnarigsg CC omarea isl be lender,aMoedel
M13Re63,haarvaTiolrds,bleor ffroomst
22559966..00000066
Sw uorsssss2 3M MN01639842
|
follonsa
following:
Tenn. River Fian/JEG
--
22/78 5/22/70
Tiusventzer
TM M3o7io~d2de0mel3lu,e##m8SCDDlazTTec,,r:aaanavvaaaciisll,aabblOleeF
ffr4ro3om2m33TTeekksmaarr
Cospaay,
Company,
P.O.
P. O.
Box
Box
D3i7s2k0e2r, Die: Cincinn&tx, O~ 45222.
D 3b3.y.lS6n11kJeecrcrmnryDi1ied4G:utAAhIIrS$iI1a--0022inhhCiigeghnhtrccaaalrrbbooRnneseesattereecelhl ccLauubttstt,linnggdssddciiereivmmeaadddee
bUy3 3JerHryichGa~itcharlleNoIntebCoeonktr#a5l 15It6e8s-e3a5rch I~bs, described
in 3M Technical Notebook #51565-35.
dizer
""VVToaordrtuteesxxiriG Geaesnn,siue""TacM M.oo,ddee)Bloh##eKKm--i53a55,00--G GN.Y,
aa1vv1aa7il1lla6a.bblloo
from
from
Scientific
Indu~trlel, Inc., Bohemia, NY 11716.
centrifuge
DCC Doaaermmnpoootanrr-i-aIIfEtuECiC[oen:M~,ooddeNeleled/#BhB--a22mO0AAH.o, igaahvviaasii,llaabbMill.ee
from
from
Damoa-IKC
Damon-IEC
Corporation, Needh*m Heights, ~A.
Botton
;
DPFoouuirr--ooauunncceeoTwwiiiddeetm maodouutsthhleeuddmtccoluleseaarTr og~ illla-~sissisebbdoottcitallepee.sseeaalleedd
with
with
aluminum foil Lad al,,mlnum foil-lined cape.
J
p1o13l25y5-s-~ae11nlllicinanepeeaa.rr ppoollyyeetthhyylleennee ((LLFPF~)) ppllass-ttlicc bboottttllee w wiitthh
polyseal caps.
4
GCOaah~sroCs~art~tooggrraapphh::
4
CCThnhrtrooong~arntatoocgogrrraapp=hhHo--lwlHlolowtoliwe-tl~Poa-PtcaekcaPlraadacrrtddModM Meoolddoe}Jl O5$77A1133 iG~ aCt.ogrator-
Integrator
-
bristor. liewlett-P~ka~'d
pr inter.
Model
33;SO&
Intogrator-
Br1ko5)t0thhvioofefe tthhWeoi1Taabbooovvuecd,~avvaPaiai1ll& aobblAleil:toff,rroommCAH flao9~w4l|3eo0tt4tt--PPaacckkaarrdd Cn. ('~..
Column - Lr~O P~l:e
Col~ln -
TMS SHiiiTlxxlh--ffoo~3o6oo4tu"t,,d,cv11l~//o88aul'~iinoacAachh~8L0OO~/DD,3,,0C"AsetC~aha9irin4onlm3leea0sss4sos. rssstteese-lAl.H,
packed
packed
CCCoaoldluuoescmatorTTeew mm =ptp~eoehrnra-atc1tuo0uSrtreuemCa--ll20aIIstsooto3thnhe0e~6r0sm0~a0/_.8ll0
Chromasorb
180[0 C. 180 C.
D PDInleeotJtweeeccct-ttooo"rrrU0--- CEcFle~leal-ccaottirlrnuooumnntneCCaoaa~pfpttu2uArr0rge0eon:aatC~M. 33e0t~0ha_neCC.. (95/3).
EFtIh~yl - A~ca0tate~/ltnUte of Ar~n:~h~e
~M "N"ULLoCtChir/l/~Be8oCC loaM MhdrA ar,aonocwnueeo`ufotliafalavvctc"e"tt:u4u5crcr3hihi1nnrr3oggo,~maC~ shtwtoteeo~aegtrCretaapatu ihypn,,7yios3s2g8oo94l#0l3vv6090ee0snH H8tit6lg~8hash8ivvKlasa.aniaidldlaubbL&lvlveee~souffuerre.o.~m
22559966..00000077
SW_MNO1639843
3M MN01639843
(79)
(9)
iaBanaddseadfatoth. the a- ctual BTraosezden onweithghet,acatudal
wweweieigighghthtt
yoeofrfcessanam tmpplolfee
used, 18.8.% euxscehd. pa1r8t.8%
less
less
thun
thmn
frozen weight, and weight ~ercent o each p~rt.
Tean. River Fish/JEG
=_,._
Tenn. Rver Flsh/JEG
55//2222//7799
W ataotre.r:
Doloazed water
Deonized water.
3. Procedure (8;
PwProroroceceeddouburtreeassineuudsseeddfrbboemellooew wa,,rlieeexxrcceep3ptMt Tffoeorcrhnm miiicnnaoolrr mRoedpiofrticastuimosnasr,ies (7).
were obtained frc~ earlier 3 Technical Report summaries (7).
S80aadm mpplplelesaece11AAd ttihhorrooluuaggrhhge 33Aalaaunamddiou11BsB ww peaersrsee, rreeimnmoov4veeddfusffreroommbootthdh,ee affraredeeezzeerr
aanldlowpeladcetdo itnhawl.arge al~lnum pane, In fume hood. and
allowed ~o Thaw.
A2A2w wdhhohololeemocgchehananinnzneeeldl
ccianattffaiissbhhl,,endss~earmmpplwleeith1l&A,,20ww0aassml ccuwutatteiInrnttoo
5
5
sections
sections
and h~mogenized In a blender with 200 ml wa~er.
3Slaaam mtpeplrleael l1BDlinhheaaddbeaahiddniindnkketerhreddiigeeillccooprreleatsseaa.m mpplleeContt~takkeeennntsjJuuosfstt thooeffff
the
the
2~Tla0e0.tp.5e5r90ro11adlucggtrrlaiamnvneeeslaoa~~rmegpbpal~lneensd,w vetehrpresaertsegkkiiollnnfl,, pkiffldaiiullte eeeyt.t,,, C asam mnoad~nltllrelencppttsa~urrstot.fooftfhebbaacckkbboonnee,,
reproductive organs, part of kidney, and rectum.
3S6a1am 1z1ppllepela33tAAe.hhaadd
Thaaeddi1lnn6kk.e8er8r4
ddigieerasccoorvreeampssalam empplcleeontttaaakkieennnedbbaeChhiilinnetdd,
the
the
hVvKooeLrmrltotleegbbeprnrlaaiaszet,ee, d. eskwkiTiinnth,h,e 1aa1n0a6dd,8m8lbbi4iloleefg.r.aVmatSSeaaurm m~pm pll1peeam lse a~11BBo"ntaatianasddsnue2eAdmizw wfeeierlrr.ese"t,
ho~offen*zed w*th 10 ml ot water In a
3wSaaom mrpeplleehos~3A4ogw ewanasiszeddditessweeiectcthteedw~,ataaennrdd. tthheeInvvdai~rvriilooduuuaasl iInpnaddriitvvsiidduuwaealilghppiaanrrtgtss
w ~ mToherroreeesehtthhoofam ~nolg22e~5s5.sn.00el~rdfgfwrre~w aim:i~gsthhtwwew erwraeeetreehhr.oomh~oog gmIneoengdaieilvzn~eiidddzuedaiInnl inpaa abbxallteae"nntddw ieseersri,g,uehw wminhihgizlleleer.'
wTaa~tblelere
a33ddllleiads~teefort:~heheomssoa~mgppolnleei,,iiissga~ mpellaeech
wwesieagmth~ ptlt.e. .
and
~d
amount
~nt
of
of
water ~d~ for h~ntzl:,g each m~ple.
dAd(Li1idvL1viiicdoodhefefrddotmthhaIieentnotto/aoababcooIfivvivdevee,e ssu~ wasllapipitqllqoeueurseoo,t,rrssikAoafafntat,ededrr ppdhblrlaoyao~,mcm eoegddoteo~nliinniunlzozamppaotrtr,iei~oocncnld,erl, eay)s~ w w.ead edrree b boottttlleess,,
oT(thdhrtceehreroatm wloiaqtwueeo/rtaey~ldwpil, raycweadptiiesrncergdtl.a~ isas, PdbKoryttG, loevtslo.l~ ewn,Sea,mWphlidelrsey)w.iedree stored
ia a refrigerator at 4.3C. until needed.
In a terra.rater at 4.5 C. until
S pSru~ empplalereeesdaan~caaollryyzzdeeiddngffootrroPF-4t~3h3e009~,)f, o=llFFoUwM-i-1n39g9323p5,r, ocwaneadddurFPeN1.--334433S22eew weTerar~eble 4
pfffooorerrrpwav eerxeeitdlgrdhatct~tcioocofofnrdsss~a~nmp~pllee~o
andthe
and
milliliters foll~In~
millllitees
of ethyl pr~edure. of ethyl
ac~ etat~ e uIssed
acetate ~
4
wAAaspprr~ewveviilooguuhs~eldl~y (hhnooom mologagerengnieizzreeddthasssaammp4pl.le0e,0, ssg)ttoorraeeaddd aiIndadeaadgBltlaaomses& 3bbo0ot-ttatllle e,, pwp Arcaeresct~i~aetlee sels~~ evedads 8g(la1nad~osdmsedlcc~ eaetnntrtrtrihifefuugr~eatettuubboefe.. 1.0AA v valoo luelmt~ehylooff aeecttehdtyyaltle pur Bgaircxaeestdaoteffow hroum1o.g5~ enmd aitneu.tesThiea eathmyilxeraceAttatae/sfpiesehd sheotmtoigaegnaotef v3ers The samples vere resoved sad centrifuged at 1800 rpm at
~e o~le8 were r~ved and ~entr~d at 1~ ~ at
22559966..00000088
3M_MNO1639844
3M MN01639844
Tosa. River Fiab/3E0 --
Tenn. River FIsh/JEG
spam
FRA rr rg TE 2211 C.. ffoorr 1200 m miLsautuetes.e.
A Afftteerr
cceennttrriiffuuggiinagg,,
the
the
othyl
ethyl
STeait oTIsM aoC mcbgr rapaiScamsaotni(ystea.ndare) Tae placed in v~al. F~ve ~1 of e~ple (standard) ws~
Injected for Ku chro~to|raph~ ~aly818.
SHamUpleBs L14,I3,4, sada18hannomeosgesssotnesB.atlpallues o8fwattheerceBnltanrka,l 8~1e8 1, 2, ~ IB h~nstem, pl~ J g~ter hick, mn LT or brani ad thoresate Fluoride In ~E ~ttlo8, uore 8en~ to J~ ~IL~Io of t~ ~ntral
m(1)o9aW TnechaicoalnRepoSrhticaSuomTarFyh.iorsvoemumsetntc30a.is13P7r8o.jecAtrch-urProepnrdess]s
(3)
Spore Cgarstrralatnasteairc,h Livoratory
Meport
Number
6603,
April
20.
(3)
~ tS"b-inohenceorlncoCn& tirta~tssiho,n"
of
.
TaU-E 4Z2 R in Dluckill
T. K1n~b~swy to ~.
TuntHizhEand
N. gelter,
in
(4)
(4)
3T0 TLIS,Apungutst May 17, 1977.
3 T~, ~ugust
111660,,
11i99t77i88.a, dAAiaXNt..tiegleoltntaorrPottooeDntD.i.alLL..ofDDaaPscoaaRa, B"
(5) "S CoEnvt~rlua&) ~ttoaT ~seonefchtheLa. BboLroactoanrcytnMl;arpsotrlotncPa oB~eoqnutel&stl ATI of Ftl-3423."
:
(8(6)
bK BEF yax peJT~~irs~e~nalti tlm a a llaew w,oorrkkAm ddTo~nLesub~inn ~ cTooorpyeAr~ aHhtEioRn
w PsiOitt,hh
wA.hoX.peWreflotremred
1. N. ~lter
(7
of
328
the
Ta ochalcal TopR rt SuY mmary,
NovoaseboTroe15.aWIrTi.edA.."Nowiet
LCilzeaan& Sop
|
| .~G/cea
22559966..00000099
au_notessds 3M MN01639845
Tena. River Pish/JEG
-o-
-9-
Tenn. River FIsh/JEG
3/22/79
5/22/79
Tame 3
FISH
WEIGHTS
AND
WATER
TABLE 3
VOLUMES
USED
FOR
HOVOGENIZATION
FISII WEIGHTS /LND IATER VOLUN]~S USED FOR HO~3CENIZATION
DesSSc~arumnipppllteeion
Description
n
IA
2
2A
1
1B
3a - mace
3A
3a
-
-
MGualslclebladder
3A
an
-
-
Laver Gall bladder
3A - Liver
3nA =-
rae
Fat
2 - pares
3A - Parts
34 - Gute
3A - Gills
IIPnorifottsiieaall wWWehhioogllneet
Frozen WeiGht
146.0 8
146.0 E
26.5
266.5 E
210.0
210.0 K
2.0 8
752.0 K
725522..00
&
E
775522..00 g
725.2.00 g
75522..00 g
775522..008g
WAAeccittguuhasltl SSUaasmmeppdllee
ml
ml
vWWeaadtteerr
WeiGht Used
Used
Whole fish (1)(2) 220000
Whole fish (1)(2)
16.684 & (3)
10
16.684
20.591
E
5
((33))
10
10
20.591 g (3)
i0
220099..9933 g
200
200
1am
10
10
1.378 g
5.99
10
10
5.949 g
52.20 6
100
5Z.2~;0
321.57 5
100
300
321.57 g
300
1199..338 g
110000
Footnotes:
((I1)) ^AfifCmiisahnwadhhoooovkka,,s
wwFiiottmhhovonnood
sDappepprnaorrrreaahttumrrouugssuttn ioorrallTiiinnoene.,,.w,w~a.x~
found
~oun(l
in
in
(2)
(2)
TRTtTohhoeeeccaofofininsesahthoatrJaa~anppFt p1p2esae0iaairrrreeuddffellodtotwowocboaboeovyveershxrliai.vi~ggeuuhhrrtftbfllaaeyycceeenddeooelfhhfeysydsffdriirsasathththe)e)iddnswoe((fprpootothzahseeensniibbaallwccyytetuuiagddalhuultcc
weiKht of fish used may ha~e huen less than frozen
((33))
SSaammppllee
ccoorree
33..6611
ccma
1d.
id.
22559966..00001100
IM_MNO1639845
3M MN01639846
Tena. Biver Plan/JEG
Tenn. River Fish/JEG
-10-
5122/79
TTAABBLLEE 44
FFIISSHH
W~EEIIGGHH"UTTSSSEDAANNFDDOREETETXHITYYRLLACAATCCIEEOTTNAASTTEE
VOLUKES
VOLUMES
USED FOR EXTRACTIONS
Desscarmipplteion
N`eSetoiggmnohtgt eaoosffteFFiisshh
3
%
TYIaatteerr
AFFEAicixcststthuhruaallW~cttt.ed
Fe Homogen~te Hosoingenste _Extr(aag)cted
cmaol3se
3am~le
(Ira.s)
Ho~olenate
EtOAC
D 3Aes-crGipaltilonBladder 1.1.2200
07.9 ws2 11.22
87.9
3A - Gall B1&dder
34 - Laver
22..3200
6622..77
s0.6 22..?2-
820.6
3A - Liver
3a - usscle
2.40
a48s.s8
1288 22.44
1228.8
2.40
3A - Muscle
an - Fa
2.40
6655..77
s23.2 22.44
823.2
2.40
3A - Fat
34 - Parts
33..0000
a48w.a3 11555511.00 33..00
3A - Parts
3 - cine
3.00
s8a3.s8
468.6.00 33..00
3.00
3A - Gills
ater Blask
2.40
110000..00
-
22..44
2.40
nwTater Blank
2.40
s57r.e8
1001228.8 22.a4
:
IA
fr)
2.40
2.40
2.7 e532 22.44
32.7
IB
2
2.40
2.40
ars 1a5s0e00.00 22.44
37.5
2A
2.40
22559966..00001111
33MM_MMNNO011663399884477
a
sw Fr
i Baa
TTEECCHHNNIICCAALL~ RREEPPOORRTT SSUUMMMMAARRYY
To: TECHNICAL COMMNICATIONS CENTER -- 201.208
impoTrEaCnHtN -ICeALrCtOMpMrU inNICaAn'bnoOtIh~sCnENTofERpap-e,2m01d-2CwNocopier fo TCC.)
#mpor~t - If report kJ Wk~d on bo~h sidm of pq~r, stud two coplm to TCC.)
[2/28/79 |
Pr~iect Re~o~t Tit~l
Environmental Laboratory (EE
Environmental Laboratory (EE
Decatur, Alabama - Tennessee
Decatur, Alabama -
FFlluuoorroocchhee~miiccaallss iinn
T Teennnneesssseeee
Tennessee
To
D. L. Bacon D. L. Bacon
----Jy5a1m5e6s8 B.. GLaagbnoRne.qu+est = #4871 > Carns commun son Runorarion)
[ CE K E YWORDS: Re a he (S~l~'t ~'rm from 3M
Thesaurus. SoUl.st ot~r
om Progress Report. z~:~i~ble terrm.|
:URRENT O~JECYIVE:
Progress Report.
DeEEEEca&&tuPPrCC
Decatur
& BC)
& PC)
River
Fish
R Riivveerr FFiisshh
River Fish
aERR > CHEMICAL REGISTRY
.
0323 76.2740
| 78-2740
100
/ ~oo
213531 NoeGr3it azerad
aE
A AT TE Ee ST Soret To mrs Corr REPORT ABSTRACT: (20~250 W~CI~ This muuact informztton is d~t~)utsd by the T~hni@ Comm~nk~donl Canlw 1o
~z 3M'e. 1o Company R&O. It is Company confider;'~ mstm'i~L
TTeSsyhyhressetetem emmniuicctrraiaoonnlwdwdiazavevccedeaappisstiuloulllssaatterarxayyiainmneieccdondoleluhhumemefnlnliiisuwuw hminitethhppxltlaareessallmecmectaactstrrddooefenntotreeccccttaafbopplrrtutuuorrreoe-
. .,,
wcCaaerrrbbeoonnutaailllicczooehhdoolltolleevvexeeallm ss..ine fish extracts for fluoro-
"~
"~
et
c:
DWD,.eRRliicctkkeeerrr--5533--44
AA..MWMeeanlnddteeellr
22559966..00001122
SW_MNO1639848
3M MN01639848
"% e
-2-
IInnttrroodduuccttiioonn::
PPtrrheeevviipooruuesssewnwocoerrkk!Iofiinvndodliiacctaaittleeedd afalunnoeereeoddchffeoomrricmmaoolrrsee. ddeeCffaiipnniiilttliiavvreey asngaassswweerrcsshrottmooastttuhooesggtrraaappiprhhneyyesdenwwciibettehhloifuaannmvoeepllllaeeactcstitmrlrsoeonnd~Tecc1taauepopctrttuouocrrrheee(mdMdiSeecHttaPeelDccs)tt.oorrwerC((eaCCpG~iCuClEEsCCle))adryaatnonddgaamsmniiacclcrryhoozrwweoaamvvae-e nesetetuhahsyrytllai3naa'eccsdeettDaahetteceelaitueeumxxrtt,rrpaalccaAttslssmaabaoofmfdaetffepiilcssahthnotrtt.aakk(eeMnnASHPfMfDrir)oonmmnweesttrohheeteaTTubeesrnneonndweensstssoeebeeu~laRlRliihyvvezeeaerrd,, ssnaaaemmabpprallceek3,,gM'rsoeeuxxDnttedrrcaaacccthttueeercdd,k.aassAlappbrreeavvmiiaoouupsslllanyyt.ddeesscAcrriMibibenedndIe,,sowwtaaassbraaollwssnoo baaunnlaalllyhyzzeeeaddd aass
a background check.
H Reessuullttss::
11.. C Caappiillllaarryy GGaass CChhrroommaattooggrraapphhyy wwiitthh E Elleeccttrroonn CCaappttuurree
NN(oosamccpoolmmeppoouu1nnM)ddsshwwaeevrrieengddreeettteeeccnttteeiddoniinntittmhheeesMMGiiInnonn8eeEssooTttoaa Tbbhrrooewwfnnlubbouurlloll-hheeasdd mcc(ihhsneeaummmtiipecclsaae,ll IssMa)ttnaadnnhdsdaaoavrlriddvnssegntr((eTTtpaaebbeallnkeetsi,oI1n))..tthiemEEexxcsccheerppcottlmoastffeooorgrrzoaaampptewe~aaaeskkfavlatteuroyr66o..-11c44lean. bmIIeinnnluocctwooemmsppa,aanrrdiiassanoobdnno,,vseolssvWaaehmmnepptlleeelssepera11kBBsDa,maa,nnddtrhee33sAApceh(c(brtbaoiasmvsssealtyaao)nngddrasccmhaaowttwaffesidisshhvemroffyrrreoommcltehaann. bd2255eloteepp~eceqaat~kkeassdn.,d--ina%basoavppeemeapakWlEheeswweiiltt1heh8r rraDneeadttmee,nn3tAtiirooe(nnsTpaetbtcilitmemeiev1e)ss.liiymm)iillaasrrhowttoeo dFFm--o66r33e0098 twwhaaasns
detected in samples IB and 3A (Table i).
TABLE 1
TABLE 1
qQUUAALLIITTAATTIIVVEE AANNAALLYYSSIISS OOFF FFIISSHH EEXXTTRRAACCTTSS FFOORR FFLLUUOORROOCCHHEEMMIICCAALLSS
SSaammppllee
"R ReettfeenntgtiioonneTtTpiiimmneeep((MM t(iMiipnpoyuuettdeens)s)
.
10.84
12.68
31wBm F313MA8u-3422 Std. FF-M6-3304922 StSdt.d.
F-6309 Std.
N-*.* D.
N NND.D D..
5
NN *. D.D..
oN.eD N.D.
N.D.
we N.D.
N*.*D*.
***
N.D.
N N+**".t.D D.0
== NNVoeotrty ddseemtteaelccltteeddamount = PVeearyk Asm reaall laemssoutnhtan standard, but greater than *
+s **
***
= P1100eakppppmmArssetataannleddasarsrddthan standard, but greater than *
22.. MMiiccrroowwaavvee SSuussttaaiinneedd HHeelliiuumm PPllaassmmaa DDeetteeccttoorr:: ((MMSSHHPPDD))
TaThhneed
and
saabubolovvfeeurssaadmmepptlleeecsstiwwoeenrreemodaaellsss.oo
sulfur detection modes.
aaTTnnhhaaeellyyzzrreeeeddssuullbbttyyssMMSSooHHbbPPttDDaaiinniieennddtthbhbyeey
ttffhhlleeuuoorr, iinnee
mmaiinccdrrooF-ww6aa3vv0ee89,ppllaaissfmmaapreddseeettneetcc,ttoorrcooounnldsshppaiivkkeeeddbesseaanmmpplldeeesstesschhtoeowdw ttfhhraaottm tFFhMMe--i33r442222
eaffxnlltduurooarrFcii-tnn6see3,09cc,oonnNtotiee~nnfttplrueoaastrteonctttahh,reebo00cn..oI1uplpepdappkmhmsavlleweeevvrebeelleenoiibnnsdeettrhthveeeecdetetethhdiyynllftrhaoaemcceettmtaachttteeeuiarl
essxaatmmrppallceetsss...2 No Zluorocarbon peakswere observed in the actual
22559966..00001133
IM_MNO1639849
3M MN01639849
Meus Discussion: hTpPFhrreeeersseeaaennbbtsoodvveeiSnn" rr1Sese5eassmmuuTppllhlitteecsssso..hiiinneddTiTiihhncceaaeatlteellaaserttrvghhgoeaaerttsalag~nnoooouuarnnvvettoo.esllaadttoouifieflleeooTor.rEffgalltnuuhiloeoecrroopccrf{aaellrrsuubbeocoornrninicsnnseeewwoeferree mnTfF BeoooonnrEmtvvoiRNEoloVranFateECtid'Tails.eein((6fef.tlEoaghui.sero,uerooonrcRFcsihChCge-aeB9imm:85ni))acelahl1wwssairssep((ohhNWriViFtFnnCddC)ee)|.rraeerddeTThbbhdiyylunen-aanLlnt.eaovyboeevvtrreehreraaccbpbhhururrnenaodsdmmaeasannnttcccooeesggrraaooopfpffhhyy iInntteergfreitryrinofg tchoempoSutnadnsd.ards:
Integrit7 of the Standards:
A TTFTAFBefHM hfrttEet-oe3e3eanr3rc4eo52sm i23ttho"hopneeeTaonrarueshniiandnnaeboiisnotouet':hiitstah.aensdllaabmyttrhhprffeeeelouorpeprroc)csor,aaoortmmuhute?1sFer'r,,Uo-1Fccmoo3~ii-inm0tt-iDp35op0a93wwogt32auaasS3ssnd(ad.tpbbsh`hrtser((yaotoN N,nauuo0-ndcgg1eaeha0thtththrhtryde"yaiunuslyppthhaTSaFanOuOddtthd-BhSaSEEaaggruttiia3vaaoraetFFl).nccnMH,oo--n3hFaa3o9S9rUo2a)d2d-lSm3)3i3ivp,f~f4flaf.a3eenen,rsrddeennaotfnt d
FFvFreeeM~Ur-ti-e3i33nf99i3i22t2aic53oatntw((iieaaeoortnien.m rr.eweesle,nnsIItbattytmypwstolaoegessaT)CKsh,ooddamacetmmhFtiooe~errnorr-rmm ec3miil9iLanaa2ontesl3feododgCrC(rahehttnpoehhahameaBittlyeocoi, nalettlldrthhhsseea,sn'MooAosmnlnltseadFdallMnyPyIcd-bUt~e3aii--4rcc532daa092"ll22.)33,LLFaSsa~sIpb-otta3am a4nnsffp2oododl2rrsaae,irrsiddtoaynf,d
BFhad
tthhee
been improperly labeled before being sent to us. In reality,
ssaam mppllee wwaass FF--66330099 ((N N--eetthhyyllppeerrfflluuoorrooooccttaanneessuullffoonnaam miiddee::
CPhApBesreL8eCpFoopBa1ffa37rrE3aa3220tt772iiNMooAAeHnnxuutE'ggouuotD)sssfe. tton`sst11ta99pa77nrn99dog,,apirerdrtatstshhyoe ((nLniaeeuduwwvenentFFiti~Ueii-.dfi33ic90coa23ao3t3t,,iiFooonnLLaoosttvvooesrr55i11lt88fi,,elaehdww)~i~)l11l aabbnneedd
uutshseeeddolffdoorr
the old
FM-3923 has been properly labeled ~s F-6309,
ReRfeeferreenncceess::
-
61GaaEggnEnoonna,,trJJoaaemmmeeossnicEEa..,,ts33M4"inTTeeTccohhnnnniiecccaaellseRReeRppioovrrettr SFSuuimmsmmhaa,rryy23""BBHiiaooyaacccc1u0u7mm5uu.ll~attlioonn
.)
of F1~orochemicals in Tennessee River Fish," 22 H~y 1979.
2a Cerober 1875. 2HHaaggeenn, D. F., 3M Technlical Report Summaryy ""AARR NNoo.. 77223388 --
ne
DDeetteerrmmiinnaattiioonn ooff FFlluuoorriinnaatteedd AAllccoohhoollss iinn FFiisshh EExxttrraaccttss,,""
p3p2e3errssOoocnntaaollberCCoomm1m9m7uu9nn.iiccaattiioonn wwiitthh AA.. MMeennddeell..
Non Shoe8, 4wWinter, L. No. 14998,
D234.4",
augur To%. Commercial
August 1979.
Chem
icalas
Analytical
Lab
Request
.
o6cL r p1s ~ G/cel
.
22559966..00001144
swNot39850 3M MN01639850
3m ~a0i~m a,,r,a,-r,i,r-Aa
A TTEECCHHNNIICCAALL RREEPPOORRTT SSUUMMM MAARRYY
[{_oectcobeor 2n233.,e310r9779
SAIN. b.4A .. (fml~ortant - If report is printed on both sides of paper, send tv~ copie~ to TCC.)
--------
eo
CENTP~L RESEARCH LABORATORIES, Analytical and Properties Research LaboratoryI 0502
Environmental Engineering and Pollution Control
{ A000007
7a
AR No. 7238 - Determination of Fluorinated Alcohols in Fish Extracts
To
J. E. GAGNON - 209-IS
Author{s)
D. F. HAGEN - 201-iS
SentSECURITY dh~1~
RR consent ~ Open
ICompany Confidential)
iiorou aussie .] Closed
ISpecial Authorization]
KEYWORDS: terms from 3M
i a 8oDl~ca hie terms,)
Central Research
Analytical Report
CURRENT OBJECTIVE:
RReeqquueesstt NNoo.. AA773311S544
Requestor- J. E. Gagnon Project No. 91500600
|t "REGRTY 3t~ CHEMIICAL >~1~
| REGISTRY
[ 23~
042608
82
gra" New Chemt,c~s Fhe Ret:)orted
Chemical Analysis
a REPORT A~;TRACT: (~0"250 words) This abstract inforn~llion ;s distributed bV the Technical Communl~atlnns Center to
alert 3M'ert to Comoanv R&D. It is Comnany confidential material.
The microwave sustained helium plasma detector system and capillary column with electron capture detoction were utilized to examlnu [ish extracts for [luorocarbon alcohol 1ove]s.
|| rg, |
22559966..00001155
3M MN01639851
CCEENNTTPR~AALL AANNAALLYYTTIICCAALL LLAABBOORRAATTOORRYY
RReeppoorrtt NNoo. ...........J28.ocemennn DaDattee............Q ~.U%.he/0_22c23,~eA_~9Wh_7T_e9.S.....
SSuubbjjeecctt:: DDeotteerrmmiinnasttiioonn ooff FFlluuosreiinnaatteedd AAllccoohhoollss iinn FFiisshh EExxttrraaccttss
Request No. AT ReRq.euqeusetstoorr:: _L_J_-_E_E: _GC_aaa.~mn.eon_n..._._._ Request No. __A_7_3_I_5_4 ........ RReeppoorrtt: IInntcreodoudcutitcooenn
DDeepptt.. NNaammee . EEHE7&CPC_______
DDaatted. _A_Au_gui_sgztt8. _1S: _91.7977......
Proj. No. 71302800
Proj. iWo. _9_1500600
aTThhneed mmCiiaccprriooluwlaaavrveye cssouulssuttmaaniinneecddhrhhoeemalltiiouvgmatapppllhaayssemnawitddheettaesccltotsootrrco((nMMPPcDDa--p88t55u00r))e--ccdhherrtooemmcatttoiopogsnraappvhheiircce susyeysistlteiezmmessd
aftnoo4d3ee0xxc2aaa5mmp,iiinnleelaandrffyiissPhhc-o3lee4uxx2mtt2rnraaccctthrsosamamamptplloeegssrapffhooyrr
the presence of Fluorocarbon alcohols MI-3923, with electron capture detection were utilized
the pre~ence of fluorocarbon alcohols FM-3923,
FM-3925, and FN-3422.
DDiissccuussssiioonnaanndd RReessuullttss
TTthhheee hhceehlleiiouvnmnazoppgllarasasmepaahicddeettpeeeccottkoorrasyyiiietellddcssluaatettsoommiifccrolmliinnteehessppceeoclctutmrrnaa. ffoorrOnetthheecaneelleetmmheeennrttessforpperreessmeeonnnttitoiirnn stsShpipeceehcciicoffhsiirccoaamtllahlletyyoglrffuaoooprrchoiffccllauutoopbrreoiiannkneeaaslaanncddoihtossleuuslll,fufuturersDettootferacaoltlmliloootwnwhe ffloocerrovleulttmshhnee.arddeeettOeneieccntttiiceooarnnnmeodotffihaetrsseeppfeeboccreiieftfiiwccemeonncciootmmFoppIrDoouunnddss adasninuddcchtaEECtCaesdddeetttbheyeecccttfoohrlresus.o.rsoacmapIIlrnnebottnshhaiitssarlictxtoy.yhppoeelsooI.fff sslDaaammerpptglleeeec,,tnioonttnhh-eeClleullvoooewrvleeisrrnedadreecettoeenccitttnaiitlooennnrimneglTdiiimnpaiietttaeksi1sbseattssweooemmeeepnwwrhheaaFsttIeDnt dotttihhceeytytahtewwiei1dlq1lubayoorvveeestrrhlelcooaaavsddiaemypttlhheeetumbppeall.taarssimmxaaT.hiaasccttIfiipvrvaealtstaieringnnegtgsnaaoln"i"-btbfytyllppeuaaossrssdlifnmseoeCddieecc""uolnttttyaooinppiirrfenevgvteehnneptteankoccsnaar-rb{aboroinenvorbpbisrunieielsleddnuutpp oifInnnnctceteehrrreeffxeetqrr.ueuanncrceteTzhenpeecaaalkkvossvietryaarreetluebaaveedd.leeqqouufTaahttieetsllhyyeprss{eeelsppueaaonrcrtaaosttceeaddclbiotffntrrlooenmalcdttoihhhefeofli(fsclluuuldootetryiitnneeeciftaccbootlnnhetetaaiinLnnoniinnn-tggfhleusppoeeeraaikkentehoofyfl iancteetraetset.{ishTheextlroawecrts le1vselabo6fve 3the nafnlougorraomcsa/r1b0o0n1 fnjoet fon alcohols detectable in these ethyl
acetate fish extracts is abou= 5 nanograms/10Nl injection.
AAd0dddi2it0tuiolonnaaallnd sseeinnnssjiiectciitvviiinctgyy 1vw0aa01ss tfoor 20t~hie WanPdO-8i3n0jeaccteingItisc0e~di
ooofbbetaatiihnnieesddcbobyynceccnootnnrccaeetnnettrraafttoiirnngganai1l00y00s~uiLis.ooff obfelotwh.is omcentrate for analysis.
Ottphheeerasstooillnuugttiioocnnondaassitirroeenccseeiivveedd
Operating conditions
for the MPD-850 are listed below.
CC1oo0ll0uummcnna SS2vy0s0sttCeemnacAA --156''C,,/at66n%%. CCWU--W22o00lMlM-u-mTTPPAAcocoornnle88r00//11a00t00 2mm5eecssch/hniCCnhh.rroonmwiGcG..h pH1u1,r.Pg7.e. ppfrraoocgegrraacmmommeeWddD fforrfoonm 50
Scc1ech0l0/rnmoiimtnnoa..io2s0e0FFaooCnrrcthyyatnppdee1rt5cc=OeeCFnn/ttmiLnoos.ff ctrtHhhaeeenlsiccluooemllruurmemcndnarreetfifoeffrlleuuheeoanntctNP2D5i3-cs8c/5sSm0plflinipt.tlawsftioomtahtthhceepauvriFF~tIIeyDD ovornniaatetthhaeetohHeHMPaPP-t-D7e7d6632o00f1/s5g1a0a6ss"
ccciashaprpioiellmallasaattrroayygtraanlLpdiihnneeaayana:dt3 611180800%00=CC.ie.setTrTshhaeelncsrcfcoaeauvrvairivettedyy Tihse~eumsitsaslinoend bLyineas iu0s0edwatftormiFclruoowraivnee
hhgteoeoaavddetrhetteesMmmaPpppDeep-rrl8aya5tt0uucrrreeepclaaiLsstsmianhhgeellcddaoveiaattt2y.022v500i000aCCpaiagannohddheeacttteehhdsee pp1ll/aa1ss6em"sa poonwdersulsfuuprplyveroeper6a3t5i6n.g0 aatnd 2.5465503.5gi|~ahreerstpze.ctively
TAMAhpupeplprLrodeoxumxiniimmsaaostntieeollntyyhel0i0.n.q5e5usaramltlu//ssameiidpnn.l,afsoraoofaffoolrx~ueyyoaggrceeitnnnoerilsastnubduuesssees.ddulafassur ttwhheeeressccaa6vv8ee56nn.gi0eerrangAdaass54to5o3.pp9rreevvAeennrttespcceaacrrtsbioovnnely.
buildup on the quartz plasma reactor tubes,
TTchhaeepcaaarbbeoovveedezsseaacmmtppilleoesns vwieenrreeanaaalltsstooemeepxxtaammiitnnoeeddlooovnner3a tccheaappiisllellnaasrriyytivcciootllyuummnnletvseyylcssctoenmfowrwiittthhh eeslloeemccpttorruoonnnds oocffaptiiunnrtceeerreedssetct..ectOiOoppneerraaittniinna~gn acctootnneddmiiptttiioonntsso lffooorrwertthheetheccaappsiiellnllsaairrtyyivisstyyysstteelmmeveaalrrsee lfloiirsstteetddhebbeecllooomwwp.ounds
CCGooollluuumamnn SScyyessmtcpeeemsraB8cuc--e330w0ammseett6ee0rrCggllaanadssss Lctcaapupniisllllaaprrryyogccrooallmuuemmdn wwasatllll10ccCoo/santtieendd. wwiitttohh 2CC4HW0--2C20.0YH.. SplIIinntiitsiimsaollde
ocoffoluiimnnnjjeeccttteiimoopnnervwaaatssureuuetwliallsiizzee6dd0=Cwwiittahhnd 9999i%t%
ofwas
of
tthpheerogiirnnajjmeemccettdeedd
atssaamm1pp0ll#eeC/m((iIinb~.1i))
thobeefi2nn4g0g~Cvv.eenntteeSddplitttao
chomode
the
22559966..00001166
3M_MNO1639852
3M MN01639852
AAORceoNvoob..er77222333,88 1979 OPcatgoobe2r 23, 1979
Page 2
aa"1ttemoocssepp/hhneeirrne.e., ofCCo9ol5l-uu3mmnArffgllooonww-nwwaeastshaaanppepprruooaxxsiinmaauttteeilllyyizei1dcccctoHHeep//uamrtiknne.. taahnenddeaalnnecaatuurxoxinilllicaaarrpyyturffello~wwdecooeffctor. 4T1hiscc/pmuirns,e olfov95-i5s Aardgdoend-maetthtahneeexwiats ouftilsihzeedcoltoumnpursgyestetmheoneletchteroHnP-5c8a4p0t.ure detector.
Thi:~ purge flow is added at the exit of ~he column system on the HP-5840.
SSFyoysrsi:eaenm1AA041=- CCfhhnrrjooemmcaatttiaoogngreoaafmn 11a00--101100--p77o99n--11soliIulltlliuuossnttrraoatfteessPH-tt3hhe9e23fflluouroorriiCnnueeF;7aaSnnOdd;Nss(uuCllHffyuu)rr rCraeeNssLppOooRnn.ss.eess NNFfoooetrsteoanttshh1eaa0tt~llattghhisrrneejeeethcetffillouuFnoolrruiioofnnreeinaeppeeI0aarkkesspsppmoaarrneesseolooubbbtssyieeorr0nvv.ee5ddofaiwwniiF.ttMhh-3t9tot2hh3eeprmomeraavjjeoonCrrtaF~aapTtceSnO662..oN55v(eCmrsHili3nna)..p.C2TTHhh~eeOHss.uullffuurr
response lags the fluorine response by 0,5 min. to prevent pen overlap.
CCehhxrrcseoammcaaectooggoerfaanm8 b11r00o--w11n00--77b99u--l22lherraeedssuullfttrssomffMrrioonmmneaasoI1t00a~4.I1 {inonjjteesccttiitoohnne ooaf[bseasnsacmmepplleeof1I-FHMluo((reetithnhyeyllcaaoccneetttaaaittneeing
Seas. extract
peaks.
of
a
brown
bullhead
from Minnesota.
Note the absence of fluorine
CG(hhereth~symmlaa~zooaggcreeataamnteI100e--xI1t00r--a77c99t--334Aoflilalluucsshetarrnaancetelesscatcthhfeeisrrheessubsllhttossve ffWoorrheaealei1r00~41iDamii).nnjjeeccttAiitoonnthoooffsesspaaommippnlleees I-A
wa"(hhreeeotrruheenydl aannatcheeootvvaeeptrrlellaoosaameddaxtrciisasacvtissthhyooowfwnt,,uabectt.hhheeanneCeelffleffalltuuceeanntattfriesapphseeaakkadobwwohvheieixccihhWstheifeshsloenwnreoovn~e--DraFfmll)uuw.oohrtelirnnAeatattteethddehosfL~essluboyrpoacsasrebdon
aaalrlcocuoonhhdoollsstheeelluupctleeasmaaanndd cattvhhieetyyy aapptppueebeaa.rt
to be sbaent. Clear areas do
to be absent.
exist
however
where
~he
fluorocarbon
CCehthhzryo0lmmaactaoocggerteaaatmne1100e--x11t0r0-a-c77t99--44of sshhsooawwmsspletthhee1-Brreess(ppbooannsssseebeoolbbtotawaiinnWeehddeelffeoorrr aDaami1)00.4~1i ~fnn.j|eecctti~oonn oof~ t~hhee
ethyl acetate extract of sample l-B (bass below Wheeler Dam).
CGofhhrroosmmaaamcptlooeggrraa1-nm3.1100--1100--7799--55 i{l1lluussttrraatceess tthhee rreessppoonnssee cobbetaatinneedd f[oorr aa 55 ffool|dd cco~nncceennttrra~tee
of sample l-B.
CCofhhrrosomanamapttlogegrraa3nm. 1100--1100--7799--66 sshhoowwss tthhee rreessppoonnssee ffoorr aa ]100u~1I iInnjfeeccttiioonn ooff a3 S3 ffooll~d ceoonnceeennctrra~tte,e
of sample 3A.
CCfhhnrrjooammcaatttioooggneraaomnfss 1u11100--1o1f00--771990--77ppmaannddsoli1u00c--ii1o00n--s7799--o88f MifNl1-ll2uu0ss2ttr)raattaeend ttPhheeI-r3re9es7s5ppoonnrsseeessspeocotbbittvaaeiilnnyee.dd ffNooorrtetthhee hcitohhnreejoelcFFotM-si-.3o39n92255ofCCs4IF~Ti,],?5Sof0O020Ni0 ((pCG~p1Hm1s))sCCb~2OuHt~lNOoHnseelluuoctfeessFM-aa3pp9pp2rr3ooxxiiamanadatceeFllMyy-39~22Samlnnrt~etseepssectaaif~vtteeelrry.tthheeNonteemethyl
homolog.
TT5hheehssaeenoglleeevaveenllsslevcceoolrrrreessppoonndd
ttoo
i100
nnaannooggrraammss
iinnjjeecctteedd
aanndd
I T
eexxppeecctt
oonnee
ccoouulldd
detect
dete~t
3
a
5 nanogram level.
CCkhhnerocowanmaattlooeggverealansm i1of00--i1t00h--e77s99e--99homi(olllllouugssstt.rraatteeIssn ttthhheeisssaacmsapspellee 20ooffngIINMowfwhhiicccahhchhhaassspebbceeieeenns svpaiskeadddweidthto
kit1nh00oa~0wni1~ ioolnffejveecssltaasemmdppllofeefor1tI--hAAetsheeaannddhaonmattolhhlyiiosssgtsswH..aass
Icnonctohnisteactaesed
concentra=ed
2v0iangevaofpoeraacthiosnpetco|e2s0u1.
via evaporation to 20~|.
1061 vere
then injected for the analysis.
~SCsayrtsteutmzes3nB r--esCpCohhnresooemmaattfoooggrrraasnmassmplI1e00s--1122I---A77,99--i11,,M,1I00--a11n22d--77199---322,,teaasnnpddectI1i00v--e11l22y--.7798--3N3otLIelilultushntetrraalttaeersett~ hseuebl~eerc~t:r~o=n ooc3ffapntccuuamarpbpeeetruutrreeeosfpssoceenonnsmssepiiottuiifnvvodeer pCpseelaaaamkkspsssl..eess TTg3hih-eveAe,ss.eeI4-aaMrrd,eeegranmenoodtet nnolef-eBcceeFssCr~ienasrrrpeilepl|coytymisvchhe.~alllyoo.TKgheeennaa~ttaoee[rdderowsst|spp~ee~pc|oi|eteasnsrt~efIcnnu~tm[t.h~h.,a,t~~w att1hh0oon2ssue1emb2eaa7rrr9ee4aaossf atnwcdhhoeemrrp1eeo0u-n1tcd2hh-ee7c3ala-lal5csc.sooehhsoollghihovoemmoollaooggdssegrwweiiellllofeelluuE=Cceeraaesspo,[{sllell.uussteTrrhaa~ttee~darrII~nnn~echh~rro|~mm.a~~tzo~~ srt~arm~s
10-12-79-4 and 10-12-79-5.
TTthhoee cccoaanpptiiallillnaarrLyyassccoolltuuhmmannn--ee0ll.ee0cc5ttrrpoopnne cbcaaasppettduurreeanrreetshseuullttasstteinnnudda|itciaaottneestcthf~aotrt ~ stahmelpe lseam!1l-~ %e ~ wvao.u|~ ldTe~ hfaevrecote atootuctoinotnasi.n laSsasmpltehsan 3-04.05andppm1-bBaaveoduldon altshoe actontteanlunativoenrsy L[iortelteh~ofs.t~he|eP~M-.192~3[oer ~e
solutions. Samples 3-^ and I-B would also ontn[n ~er~. l|ttle of the ~-3~5 er
22559966..00001177
3M_MNO1639853
3M MN01639853
AAORccoNNobo..er77222323,88 1979
|
OPcatgoebe3r 23, 1979
Page 3
|| PI-3422 species. These laccer tuo saaplen do have a peak at the recencion clue oFoCM~fh-r.o~R3mti4a--2c233o99n22sr33paenmc.miaaejjsoo.rrLowiiTesshrooemmseelerrevbbeluulattsttetotrhfheedtewifotsaeoocsmmteaerimrpolnddetisssvttidraroiibbeuuhltatelvicoeotnrnoanifpssecanankooptttautreeevviitddhweeeonnuttlrdetiiennrnetttqihhuoeeinrseamapdldeitional ScahmrpolmeatoCglreaamn.sp Lporwieorr loevcelhsromofacodReEtaepchtyi.on via electron capture would require addit~onal
sample cleanup prior to chromatography.
TTthhheeeserreessauulllcttossholoosbbttaaLiiEnneepddrebbsyyentcthheceoummliidccrrohowaaavvveee hpapallnaassmedaaeteddceettteeedccttoofrrroooann cshspopiiikrkeedd(lssuaanemeppillneeess cossnhhtooewwnttthhaaattt tttInhhh'eeesce00h..e1IalaccppotpphumaolllLseevvseeaillfmplpiiernnse.sttehhneet eecctohhuyyllld aachcaeevtteaatteebeeeen~xttdrreaacctttessc..ted NNooFroffmlluutoohrrooecciaar rrbb[oolnnuorppeleanakeksscwovenertreeentohbsase~errvweedd
in ~he actual samples.
0D.. F. HHaaggeenn
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DFH/rs
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