Document aJMNZV3OyNBN34mGpbRa0R07b
ACUTE TOXICITY TO FISH
TEST SUBSTANCE
Identity:A mixturecontainingperfluorooctanesulfbnatweh,ich may alsobe referredtoas PFOS, FC-95, or as a component of FC-201. (IOctanesulfoniaccid,perfluoroalksyullfonatesalts(5)+ (5140P)) (CAS # 2795-39-3)
Remarks: The 3M productionlotnumber was Lot1. The testsample is FC-201. Itspuritywas not sufficientclhyaracterizedt,hough current informationindicatesitisa mixtureof6% PFOS, 36% diethyleneglycolbutyl ether,36.44% water,12% Sultonefoamer,9% sodium octylsulfate0,.5% sodium laurylsulfatea,nd 0.06% benzotriazole.
The followingsummary applies to a mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflectthe toxicityof the fluorochemical component of the testsample.
METHOD:
Method: Not given.
Type: Staticacute
GLP: No
Year completed: 1975
Species: Pimephalespromeias
Supplier:Not given.
Analyticalmonitoring: Temperature,pH, and DO
Exposure period:96-hours Statisticamlethods: LC5o valuescalculateudsinggraphicinterpolation,
recalculatedusing probitanalysis.
Test fishage: Juveniles
Length and weight:
Average length 2 in.
Average weight 1.5g (wet)
Loading: Not given.
Pretreatment:None.
Test conditions: Dilutionwater: Carbon filtereSdt.Paulcitywater,St.Paul,MN
Dilutionwater chemistry: Not given.
Lighting:Not given.
Stock and testsolutionpreparation:Not given.
Concentrationsdosing rate:Once
Stabilitoyfthe testchemical solutions:Not noted
Exposure vessels: Not given.
Number of mplicates: 1
Number of fishper replicate:Not given.
Number of concentrations:fiveplusa blankcontrol
Water chemistryduringthe study:
pH range(0-9h6oum): 7.0- 7.0(contreoxlposure) 6.8- 6.9(750mg/L exposure)*
Temperature range (0-96hours): 69 -70 "F
Dissolved oxygen range (0-96hours): 4.7- 5.6mg/L (controelxposure) 4.0- 5.0mg/L (750mg/L exposure)*
750 mg/L (secondhighestconcentratione)xposurevaluesused because thehighestconcentratiounsed resultedintotalmortalitbyy 72 hours.
RESULTS
Nominal concentrations:Bk control1,00,250,500,750,and 1,000mg/L Element values: 96-hourLC50 = 526 (397- 631) mg/L
Element valuesbased on nominalconcentrations
Remarks: Testingwas conductedon a mixtureas describedintheTest Substance Remarks field.The valuesreportedapplytothatmixtureand not thefluorochemicaplroportionalone.
CONCLUSIONS
The testsubstance 96-hourLC50forfatheadminnow was determinedto be 526 nig/Lwitha 95% confidenceintervaolf397 to 631 nig/L.
Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133
DATA QUALFTY
ReliabilitKyJ:imischranldng3. Testinglacksrecordofmethodology used. The sample puritywas notproperlycharacterizeadnd thestudylacks analyticaclonfirmatioonftheamount offluorochemicaplroportioinnthe solution.
REFERENCES
The testwas conductedby the 3M Company, EnvironmentalLaboratoryS,t. Paul,MN, Lab Request number 2563, 1975.
OTHER
Last changed: 6/26/00
-FORM
106 20-PWO
@--c-c-.TABREQUEST BOOK - 42-5W
Water and WastoWater REQUEST FOR LABORATORY
WORK
File: LabRequestNo.
TO: ENVIRONMENTAL
ENGINEERING
Requestor
Name:W2 l@@(M@A Request Date
ProjectNo.
Prob. N
Code
Description
Sample Date:
Date Needed:
HoldSample For-Days
Est.Time:
H rs.
LABORATORY
- BUILDING 53-6N
Lab Personnel
Date Rec'd Analyst:
y
P-
Date Completed:
Hrs.Spent:
Sample Description
Total Hardness,mg/i asCaC03 Calcium Hardness,mgA as CaC03 7'F-"'Alkalinimtgy/,i asCaC03 "M" Alkalinitym,g/i asCaC03 pH Sulfate,mg/i as S04 Nitratem,g/i as N03 Chloride,mg/l as Cl Silicam,g/i as Si02 Fluoride,mg/i as F Ortho Phosphate,mg/i as P Total Phosphate,mg/l as P Total Chromium, mg/i asCr Hex. Chromium, mg/i as Cr Total Cyanide,mg/l asCN Ferrocyanide,mg/i as CN Iron,mg/i as Fe Manganese, mg/l as Mn Surfactant,mg/l as-LAS Phenol,mg/l BOD, mg/l COD, mg/l (Short,Long,Dilute) TotalSuspended Solids,mg/i VolatileSuspended Solids,mg/i Turbidity,mg/l Silica TotalSolids,mg/i Total VolatileSolids,mg/l Conductivity,mhos Total Bacteria,No.7100 mi TotalColiform,No./100 mi FecalColiform,No./100 ml FecalStreptococcus,No./100 mi Ammonia Nitrogen,mg/ias N KiedidahlNitrogen,mg/i as N Oil and Grease, m@/1
Comments: 0
White - Environmental Engrg. Lab Canary - Environmental Engrg. Lab Pink
Environmental Engrg. Lab Goldenrod
3M
- Requesters Copy
01
F.NVIRCWWCAI EMDOMING' IAB^'O"RATORY
@WATIC TOXICITY WORK SHUT,:
Type Test % Hours Test Organism Fathoad Minnow
g4
L
Material Teste'd Fr-- gi
Avg. - - -
Avg. Weight
-Size lid
Date Started
1-7-75-
Date Completed
Time Started Dilution Water Carbon Filtered
Plotted L4C Sd
St. -P-aIu City water
Conc.
24 hrs D.0
Survival _pH ppm
49 hrs D.0
Survival pH iom
72 hrs %
Survival pH ppo@
D. 0 SuTvi va I -@PH PPM
Control 100 -7615.4 too
loopel 100 7.ol
-7.ol,5.\ 106 7.6.
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Average Temperature
70
Comments.
461
70
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