Document aJ58NvzjyN3Gndxd1Kyp5Nor9
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE
ARI 0510
Identity: P21es1r6Pf,lFuFOoCr-Ao1o2ac6mt,amnFooCni-ci1ua6cm9i,ds,aolr`t,aFmACm-mo1mn4o3in.umi(usOamclttp;aenmrofaiiycuoaarcloisodoc,tbaencraetfee,rrFedC-to pentadecafluoro-, ammonium salt, CAS # 3825-26-1)
Remarks: 3M production lot number 390. The test sample is FC-126,a
white powdery solid. It's purity was not sufficiently characterized, though
7cu-rr2e2nt%iCnsf,orCmsa,tiaonndiCnd7ipceartfeisuoitrios aanmailxotguuree cofom7p8o-un9d3s%.test substance and
METHOD:
TMeestthotdy:peO: EStCaDtic2a0c9ute
AYGneLaaPlr:ytciNocmoapllmeotneidt:or1i9n8g7: Dissolved oxygen concentrations.
TmeestrtoorWagsatneiwsamtesrouTrrceea:tmAecnttivPaltaendt,sSltudPgaeulm,iMxNe.d
liUqsueordctohlelesctaemdefrdoamy
the as
Tcoelsletctcioonnd.itions: Dilution water:
Aerated,
distilled,
deionized water
TSyenstthveetsiscelsse:waNgoet:stpaetredOECD 209
Nsuumbsbtearncoefacnodnc2enbtlraantkioconnst:rol5s.plus one reference
TMeisxtetdelmipqeuroartSuurse:pe2n0d-e2d1SoliCds: 2.7 g/L
pHof Activated Sludge Mixed Liquor: 7.8 (initial) and 8.0
(final)
(pfHinaolf) 1000 mg/L test concentration: 7.4 (initial) and 8.1
Exposure period: 30-minutes and3-hours
wDRieerfweeatrceerrne,caet1e6adnmbdLytsaeydsndttihsneogtliuictndsiioevnwidapurgaelepwafereiaegdth,itoasnn:tdoA2sl0lol0tuetsmitLosnsuinbcosoctnuatlnauicmne.isnogTlhu2te8i4opnHmsLof
these solutions was then adjusted to 7.0 using 1N NaOH. Reference solutions were created using a 500 mg/L stock solution of 35dichlorophenol
03854
Element basis: Respiration inhibition as determined by dissolved oxygen consumption.
RESULTS
rr------
Nominal concentrations: Two Blank controls, 100, 180, 320, 560, and 1000 mglL test materia! solution.
Percent Inhibition in Respiration Rate
CoTnosctenSturbasttiaoncnes 3E0x-pMoisnuurtee
Como [Como
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se0 o
[woo
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ur
az]
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iar|
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Element values: 33-0h-omuirnuEtCe sEC=so>=10>0100m0g0/mLg/L
tCownotcroonltrroelsspfoornstehe: 3A0d-emqiunauttee 2ex.p2os%urdeifafnedre0n.ce8%indriefsfpeirreantcieoninrarteespoifratthieon rate of the two controls for the 3-hour exposure.
CONCLUSIONS
aTfhteert3es-thomuartseroifalexipnodsuucreed. 38% inhibition in respiration rate at 1000 mg/L.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331,
St. Paul, Minnesota, 55133
DATA QUALITY
Reliability: Kiimisch ranking 2. This study meets the criteria for quality
testing. However, it lacks characterization of the test substance purity and analytical confirmation of test substance concentrations. No results were given for the reference compound.
REFERENCES
C03855
TLhaibsorsattuodryy,wSats. cPaounld,ucMtNe,dLbaybtRheeq3uMesCtonmupmabneyr, EE1n2v6i2r.onmental OTHER
Last changed: 5/24/00
03856
"Let
ENVIRONMENTAL LABORATORY
ACTIVATED SLUDGE MIXED LIQUOR
E Ww J2RR
Dmantale4ye-r2S0A-R27
Type Test Achwated Slo a Rec cation Tohibition ozen r20q
source Mescn Uiacte Treatment Plant St Pavol mw
Date Obtained 4-20-87
Date Vsed__4-30-@7
Mixed Liquor Suspended Solids (MLSS) A. 7 g/L Adjustment fone g/L
PAI2n8iFt inau lsl 80:
Comments,
------------------
Test Substance Sample Description FC -)2L Lot 390
SamplePMOLbyghesttons scalesfu MIustaent_ANsersuns. 102.0
Comments_ Tnauudua cabs
Reference Substance
sample Description _3,.5= Du hlosapheaa
Semple pi 2.3
Adjustment
Comments_Stock Solution
S00 bag Je
Alone
Exposure Conditions Test Duration 3- Hpors Test Temperature 20-21 c
Coments_Teot ran Guth
LR EJARA
000000000
et e --------------------0-- 3857
[Ip o
Lbrectuo,rE ARR
Environmental Lab -- WorkSheet
sumpepmeipion Achiaied Slodae Respiration
a
one: H=20-87
FC-12b L310 T\(hgion Test OLD #209 am SA
-white powdery
eld 7
tes Solomens Prepacabion
Te Goa
00 CSTweer pesREE PE EEeR Tmhtjul
So + 2%
+
le
+ doo = S00
180
q0
4
+
1
+
400 = Soo
20
leo +
o
280 +
+
+
Ib
+ 400 = soo
+ Zoo = Sco
0a0ni0(25
sop + agq +
Jb
+ Aco - Soo
=
oO
ee,
e--e------
Orindusweights. Appeared so chiosalie Completely
T;
sone. seed, toooolly = bok
---- -- 1 ----------
nN
T bared sl LR EIA81)_FC-uux ls -lol Test, ds
-
Cento L
te eee--
-- -- ,------ ee ------e------
--------------e------------
PLwater weeds
a_i e-- --y_-- ------ -_------------_-- ------ --------r ea
03858
WE IAQ
i
acrivaTEDsuassriation amsrrion 1st oan rags POX BD
RAW DATA
en sons Ee t2l Las 200 Source of Activated Sludge Mixed Liquor:
J)
Smee ha 30 - MIN. EXPOSURE 2-0mom I Test Substance
"TIHE-MINUTES
nl MAS
[emg
Concentration
JL)
0.5 1.0/2.0] 30)40] 5016.0] 7.0 8.0129.0]10.0
--
bolo 5s]1elit[3alae]aifa]]i Ja
>
lsa[sslesldi|a4]27]20]13]o[|s] [a
2 a5a[[uyaal]i9laaaa[ll133soo5aesffaiZll8]al||sa 1aal
co on
ellel5d2s]saalls aslaalep[aA3l2aLaealllzeaa]F]ad1lJaaolsas
oman I eileiI lzrzolralbelaalzaliBsatloar
3 - HOUR EXPOSURE
v il DISSOLVED OXYGEN CONCENTRATIONS mg/L
p-- Concentration 7.4[7.20 2fe.a]ss. [te [hl (mg/L)| 0.5 1.0 | 2.0] 3.0] 4.0f 5.04 6.0 | 7.0 slofsa 8.0} 9.0 10.0 .
2l3e0o JJ sulsaalraerzoell desl[zeest[|5a eeallssl ts[shaer lltie fb [a[s.ce]ao
Sto bazar lasses fu ols
/ oon gsles|2.3]g
PE
wen La L TTr T ST HEa T iglneT l7b 15] ola
48 of fottented Stutee Hieed Ligue Toupee 713
po AEE
BH of highest concentration tested Trial = 7.4
Test Temperature 20-21 C
Finals 8:
Sossse
Lo Ce -
R_E]2ga
ACTIVATED
SLUDGE
ENVIRONMENTAL LABORATORY RESPIRATION INHIBITION TEST
OECD
#209etre
gE
CALCULATION OF RESULTS
a3 BHI
Test Substance Lo_y_ 24 _0__E 00( 00C 000- 00j 00A 00 L,
30 - WINUTE EXPOSURE
ConcoeanttraStiuobnastance (ng conTRoL
20 20 S20 cco
Geygen Uptake Tate] R 2 Zo [7 RTehsipoiirtaitoinonTnRate (1)
0.7332 lo.Lai|%
=
708 low rps] I
0.714 PT -
ot
lo. e350] 0
0.bost lo. ost]
co 442 luo gue| 29
ConTor
708 EWETEN =
Percent Difference in Controls 3) 2 2 Up
EI CS0 (95 X ConfidenceI Lintts) mg/l 8s2w,eSLzOo,wfocomyi
3 - HOUR EXPOSURE
CasmoamIseinod
------------------.
Test Substance
Concentrations L)
control o gc
330 o
Gxygen Uptake Rate|R Cornuied| TahIbToICIon
R
to ape
Respiration Rate (1)
0. 5255 |o43dend
-
o.
= lo.4ga|g
X
4.4800 |4o goo|
7
0.133 lo4333] I
o. 4400
o. s lo.314|5
2
covTRoL 1
0.529
lo 5059| -
Percent Difference tn Comcrole 0.5
BC30 (957 Confidence Linica) mg/L 37, 569, 1000 mgiL
----1+-- 5 zcsoG=isotiurasy Se_s,--Pas 4 Sor Gok rect
(1) 2 Iabibieton =Efro =)- -- mail sein . Tprep. Sew
Taempp. Cotcted. toSa 202gC 11
Where: RB== OCxgyegnen-sUUpptaakka|eRate (ng O,2/L/Kin.),
--Lo*eks| ="mOge[L,perTamiyn at Roc
@ 2 Difference =(RES1_i*Rs C3) * 100
CByi=rCooentrrol I C,= Control0II3860
The tSwo hBK CoLnottroolf rCerspirad ttionnrro antes su hould d