Document aD40kxraXQZwEEDDa3w2zQBvy
TOXICITY TO AQUATIC PLANTS
TEST SUBSTANCE
Identity:A mixturecontainingperfluorooetanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, or as a component of FC-3017. (1Octanesulfoniaccid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber was notnoted. The testsample isFC-3017. CurrentinformationindicatesFC-3017 isa mixtureof0.67% PFOS, 20% diethyleneglycolbutylether,55.41% water,21% ethyleneglycol, 1.33% Hydroxy foamer,1% sodium octylsulfate0,.5% polyoxyethylene monooctylphenylether,0.04% sodium laurylsulfatea,nd 0.05% tolyltraizole.
The followingsummary applies to a mixture with incompletely characterizedconcentrations of impurities.Data may not accurately reflecttoxicityof the tluorochemical component of the testsample.
METHOD:
Method: OECD Guideline201 and EPA CFR 40 797.1050. Type: Acute Static GLP: No Year study performed: 1996 Species: Selenastrumcapricomutum Source: In-housecultures.OriginallsytockfromATCC, RockvilleM,D Element basis: AJgalcellcounts(cells/mis)p,ecifigcrowthrates. Exposure period: 96 hours StatisticaMlethods: Not applicabldeue tostudyresults. Analyticalmonitoring: Not given.
Test Conditions:
AlgalNutrientMedium: Nutrienntiediumforfreshwateralgaeper OECD method. The pH ofthismedium was 7.9. Stock and testsolutionpreparation: A 1000 mg/L primarystocksolution was prepared by dilutin5g00 mg oftestsubstance in500 mL ofstandard algalnutrienntiedium. Aliquotswere then added directltyodilutiownater in testvesselsto createtestsolutions. Exposure vessels: Not given Agitation: Not given Number of replicates:Not given Initiaclellloading: 1 X 104 cells/mL Number of concentrations: one plusa blankcontrol Lighting: Continuous Water Chemistry: Not given.
RESULTS
NominalconcentrationsB:lankcontroalnd 1000mg/L
AlgalGrowthResponse E,,Cwvalues
Exposure (Contact)
Hours 24 48 72 96
Mean PercentChange in
SpecifiGcrowth Rate
(1000mg/L Conc.)
15
9.1
16
1
17
EC50 (mg/L)
>1000 >1000 >1000 >1000
Element valuesare based on nominal concentrations.
Remarks: Testingwas conducted on the mixtureas describedintheTest Substance Remarks field.The values reportedapplytothatmixtureand not the fluorochemicalproportionalone.
CONCLUSIONS FC-3017 exhibitsa 96-hour ErC5oforspecificgrowth rateof >1000 mg/L.
Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133
DATA QUALITY
Reliability:Klimischranking= 4. Not alllaboratorydata couldbe located. Study lacksdescriptioannd recordsofthe method followed.Sample purity was not properlycharacterizedand the studylacksanalyticaclonfirmationof the amount offluorochemicalproportioninthe solution.
REFERENCES
Thistestwas conducted by 3M Company, EnvironmentalLaboratory,St. Paul,MN, Lab Request number N3188, 1996.
OTHER
Last changed: 6/26/00
05/30/2000
ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT
LAB REQUEST NO. N3188 REQUESTOR NAME: SBS/BOB COX DEPARTMENT: 3058 PROJECT NO: BILTWENVIR DATE RECEIVED: 09/20/1995 DESC: FC-3017 (LIGHT WATER)
CONTRACT LAB(S): ASCI EXP COMP DATE: 11/22/1995 DATE COMPLETED: 05/23/1996 PROJECT LEAD: SA BEACH PHONE NO: 651-778-7452 3M FAX NO: 651-778-6176
Standard set of tests. Please make this a priority project as Europe needs data as quickly as possible without incurring additional costs. Please also copy Agnes Vidal and Rene Lehenaff on testing results. MSDS Doc.# 10-3922-1.
BOD and COD will be put on another lab request. sab 9/28/95
Results from Test Conc. mg/l 125 250 500 1000 EC50 95% CI sab 9/28/95
Microtox Test: Percent
5-min exp. 6.5 16 26 44
> 1000
Light Loss 15-min exp. 9.5 22 37 57 780 (730 - 850)
30-min exp. 14 28 46 67
560 (540 - 570)
Results from 96-hr Fathead Minnow Toxicity Test:
Test was run as a static-renewal. Beakers were renewed at 48 and 72-hrs
of exposure. This was done because the dissolved oxygen levels dropped
below the required 60% of saturation. (1000 mg/L renewed at both times,
100 mg/L renewed only at 72 hours. sab 10/27/95
Test Conc., mg/L 1.0 10 100 1000 Duplicate 1000
Percent 24-hrs exp.
0 0 0 0 0
Mortality 48-hrs exp. 0 10 0 0 0
72-hrs exp. 0 10 0 0 0
96-hrs exp. 0 10 0 0 0
LC50
>1000
>1000
>1000
>1000
NOTE: The blank controls and 1000 mg/L concentrations were duplicated; this test combined a range finder with a definitive test at 1000 mg/L. sab 10/27/95
Results from Activated Sludge Respiration Inhibition Test:
Test Conc., mg/L 1000 Dup. 1000 EC50
Percent 30-min Exp.
0 0 > 1000
Inhibition in Respiration 3-hr Exp. 0 0 > 1000
Rate
No significant inhibition in respiration rate was seen after 30-minutes and 3-hours of exposure to 1000 mg/L.
sab 10/17/95
BOD AND COD ARE ON LR N3688. sab 11/27/95
Results from algae toxicity test:
Test Conc. mg/L 1000
Mean Percent change in Specific Growth Rate
24-hrs
48-hrs
72-hrs
96-hrs
15
9.1
16
17
EC50 sab 1/16/96
>1000
>1000
>1000
>1000
Results from Daphnia magna acute toxicity test:
Test Conc. mg/L 1000 (all 4 reps)
Percent Immobilization
24-hrs Exp. 0
48-hrs Exp. 0
EC50 sab 5/22/96
>1000
>1000
--------------------------------------------------------------------------------
SAMPLE DATE
CODE
DESCRIPTION
RESULT
MDL or (95% C.I.)
--------------------------------------------------------------------------------
CONTRACT LAB DATA
05/30/2000
ENVIRONMENTAL LABORATORY
2
ARCHIVED FINAL REPORT
LAB REQUEST NO. N3188
CONTINUED
--------------------------------------------------------------------------------
SAMPLE DATE
CODE
DESCRIPTION
RESULT
MDL or (95% C.I.)
--------------------------------------------------------------------------------
1
FC-3017
Amber Liquid
ASCI 48-HR-EC50 *DAPHNIA ACUTE TEST
96-HR-LC50 FISH ACUTE TEST - 96 HOUR
IC50-ALGAE ALGAE ASSAY - IC50
MICROTOX
MICROTOX - EC50
SLUDG-RESP SLUDGE RESPIRATION - EC50
>1000 MG/L >1000 MG/L >1000 MG/L 560 MG/L >1000 MG/L
(540 570)
ENVIRONMENTAL LABORATORY
PREPARATION WORKSHEET FOR FRESHWATER ALGA GROWTH INHIBITION TESr'
LR SEE BELOW PAGE OF DA I AN
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@tiT!elsl-tmet c6nforwmislthhefo0owitnwaolest ECD #M I @Alga,'GrowtlhnhibdioTnesr and Fed.Reg:Vol.40.No:797.1050't2'A
STANDARD
ENVIRONMENTAL LABORATORY NUTRIENT MEDIUM FOR FRESHWATER
ALGAE
NUTRIENT
CONCENTRATION IN STOCK SOLUTION
FINAL CONCENTRATION IN TEST SOLUTION
STOCK SOLUTION 1: MACRONUTRIENTS
NH4CI MgC6 6H,,O CaC6 2H20 MGSO, 7H20 KH,PO,
1.5 g@t 1.2 g4. 1.8 g4. 1.5 giL 0.16gA.
STOCK SOLUTION 2: FE-EDTA
FeCl, -6H20 Na2EDTA -2H20
80 mgfl 100 mg/L
STOCK SOLUTION 3: TRACE ELEMENTS
H,BO, MnC6 4H20 ZnC6 CoC6 6H20 CUC6 -aw Na2MoO,, 2H20
185 M91L 415 mgtl
3 mgA. 1.5 mgtl 0.01ffo& 7 mgtl
STOCK SOLUTION 4: NaHC03
15 mg(L 12 mg/L 18 mgfl 15 mgfl 1.6mg(L
80 ug/L 100 ugIL
185 ugil 415 ug/L
3 ug41.5 ug40.01ugfl 7 ugil
NAHCO,
50 g/L
SterHkzsetocksokftm 14 by membrane riftrati(o0n.2um).
SOLUTIONS. NUTRIENT CONCENTRATE
50 mg/L
100 ML ofSTOCK SOLUTION
..l.ci.o-tmSLTOCK SOLUTION 2; -10M 0fST.O.I.C.K.SOLUTION3;" -'10rnl'ofSTOCK S CLUTION 4.,:,'.
LR M1655 N3188 N3194 N3281
L NUTRIENT MEDIU m
ju umerdt. D
ntrate
ENVIRONMENTAL LABORATORY .
FRESHWATER ALGA GROWTH INHIBIT10k T'E'S SPECIFIC GROWTH RATE (341
-C "X
ALGAL SPECIES: Freshwater Green Algae, Solonastrum capricomutum.--@-,@@@.@.,..
DATEINITIATED:181qlgSli.
o DATE TERIWINATEO: 1011344-1:rt(ooo
ys
TEST SUBSTANCE CONCENTRATION mg/L
24 HRS
MEANORRATE
%C"ANGER
48 HRS
MEANORRATE
%CHANGE"
@72
MEANGRRATE
CHANGE*
MEAN OR gift
BK CONTROL
Z. 104
.4
STD NUTRIENT
M1655 1000 mg/L
1.988
1.2.97, z 7-
ILVIO
N3188 9.1
1000 nV/L
N3194
100M0gA-
o.sql
05
N3281
1000 MgA-
1.841
I.-Oril 14
DATE/ANALYST Testmothodokw conkxmsvalhthefolowwltgwolostprotoook)
OECD #201 *Alga.Growth lnh&bon Tear and Fed.Reg,Vol.40, No.797.1050,*AlgalAcuteToxicitTyesr, Speak &owth Asia (u)- &nN,-InN,4W.here N,Isdw ffmanmeastoodfinacle*densityN; Isthelnifaclalldonsityand tIsthetim ofthenisasurwnenl PercentChange inGrowth Rate% (L40anControlVaka -K*an sampie vmmywan ControlVaka x 100.