Document aBbX8Z33Zk3OyvnMDYx7rYNvb

/WU06-OS33 BIODEGRADATION TEST SUBSTANCE Identity: Perfluorooctylsulfonate, didecyldimethylammonium salt; may also be referred to as Fluoroalkyl ammonium derivative. [1Decaminium, N-decyl-N,N-dimethyl-, salt with 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecafluoro-loctanesulfonic acid (1:1), CAS # 251099-16-8] Remarks: The 3M production lot number was Lot 1. The test sample is L-14394 referred to by the test laboratory as P3025. The sample was labeled F-11615, Lot 1. The test sample is a mixture of the test substance in water (approximately 30-40% test substance, 60-70% water, and 0-5% of residual perfluorochemicals). All values reported relate to this mixture. The test sample appears to be a 2-phase dispersion (clear liquid with opaque solid) which rapidly separates after agitation. No calculations were made to adjust for the actual concentration of the test substance in the test sample. METHOD: Method: OECD Method 301D Type: Aerobic GLP: No Year completed: 1997 Contact time: 28 days Inoculum: Polyseed- NX (with nitrification inhibitor), lot A306, Polybac Corp, Bethlehem, PA RESULTS COD: 125,000 mg O2 / kg chemical CBOD Exposure period, Day 5 10 20 28 First Replicate CBOD Mean Value (mg/kg) <40,000 <40,000 <40,000 55,039 Second Replicate CBOD Mean Value (mg/kg) <40,000 <40,000 <40,000 <40,000 C4222 Remarks: Values reported are for the test sample. No calculations were made to adjust for the concentration of the test substance in the test sample. CONCLUSIONS This testing indicated that some portion of the test sample biodegraded in aerobic environments capable of supporting biodegradation. This study cannot rule out the possibility that conditions could be found that would allow for biodegradation of the fluorochemical component, nor could it exclude the possibility that a microorganism could eventually be enriched that could metabolically alter this chemical. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking 2. This study lacks sufficient characterization of the test substance purity and analytical measurement of test concentrations. Because a mixture was tested, the testing cannot conclude what portions of the test sample were responsible for the apparent biodegradation values. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, St Paul, MN, Lab Request number P3025. OTHER Last changed: 5/24/00 C04223 3M ENVIRONMENTAL LABORATORY DETERMINATION OF THE 28-DAY CARBONACEOUS BIOCHEMCIAL OXYGEN DEMAND (CBOD) FOR P3025 OECD Closed Bottle Test, #301D (Modified) STUDY COMPLETED: March 28, 1997 FINAL REPORT COMPLETED: May 20,1997 Env. Lab Request Number P3025 Prepared by: Name: Title: Laboratory: Address: Susan A. Beach Advanced Environmental Biologist 3M Environmental Laboratory Building 2-3E-09 935 Bush Avenue St Paul, MN 55144 004224 28-Day CBOD Page 1 May 20, 1997 SUMMABY In this study the aerobic, aquatic ready biodegradability of P3025 in general accordance with the "Closed Bottle Test", OECD Method No. 301 D was determined. The test substance was found to be not readily biodegradable with removal values of <32% and 44% (duplicate) obtained after 28 days. OBJECTIVE The purpose of this study was to evaluate the ready biodegradability of P3025 after 5,10, 20, and 28 days of exposure using a modified form of OECD Method 301 D. MATERIALS AND METHODS Test Substance: P3025, date 10/7/96, Lot 1 Appearance Aqueous Solubility Two-phase dispersion (clear liquid with opaque solid) Apparently not completely soluble in water at tested concentrations. Available Physicochemical Properties Boiling Point: Vapor Pressure: Vapor Density: Evaporation Rate: Solubility in water: Specific Gravity: Percent Volatile: pH: 100C ca. 18 mm Hg @ 20C <1 <1 Insoluble ca. 1.2 60 - 70% 6-8 Date Received 10/16/96 Storage In the dark at ambient room temperature (22 2C) Test Solutions Test solutions were prepared as Water-Accommodated Fractions (WAFs). Four stock solutions, each in duplicate, of approximately 6, 12, 25, and 50 mg/L were prepared in aerated mineral nutrient media. Each was capped and allowed to mix overnight, in the dark, at ambient room temperature. Test substance was apparently not completely miscible with water. A few white particulates were observed in each stock solution after mixing. The clear liquid portion was withdrawn for biodegradation testing. Reported values The reported values were based on nominal concentrations in the test media at the beginning of the toxicity test. 004225 28-Day CBOD Page 2 May 20, 1997 Test Organisms: Type Source Lot number Preparation Polyseed-NX (with nitrification inhibitor) Polybac Corp., Bethlehem, PA A306 Contents of one Polyseed-NX capsule placed into 500 mL aerated mineral media. Solution stirred for sixty minutes on magnetic mixer. Solution kept stirring while aliquots withdrawn to inoculate test bottles. Test Conditions: Photoperiod Test Vessels Test Volume Loading No. Replicates Mineral Medium 24-hours dark 300 mL clear glass Wheaton BOD bottles with ground glass stoppers sealed with a water seal and capped. 300 mL 5 mL reconstituted inoculum added to each bottle except for media blanks which received none 2 each for medium blank, seeded blank, reference standard and each test concentration. Only one replicate for toxicity check. Prepared with Hach BOD Nutrient Buffer Pillows (catalogue # 14863-98) each dissolved in 19 liters Millipore Milli-Q water. Glass carboys aerated with laboratory air filtered through a 0.2 pm filter for approximately 2hours. Carboy openings covered with new foam plugs and allowed to stand overnight in the dark at ambient room temperature. Initial Stock Solution Loadings nominal First Replicate 6.7 mg/L 12.3 mg/L 26.9 mg/L 51.6 mg/L Duplicate 5.6 mg/L 10.9 mg/L 24.3 mg/L 54.2 mg/L Test Bottles Each test BOD bottle was seeded and filled completely full with solution from the appropriate water-accommodated fraction. Reference Standard Toxicity Check Glucose/glutamic acid mixture. Hach BOD standard solution ampules, cat. # 14865-10. Added 1.5 mL of solution to each reference standard BOD bottle. Added 1.5 mL of Hach glucose/glutamic acid mixture to a seeded BOD bottle containing the test substance P3025 at the highest loading. '04226 28-Day CBOD Page 3 May 20, 1997 Test Temp., C Stock Solutions pH Exposure Period Measurements Date Test Initiated Date Test Terminated Procedure 21 - 22C, continuously recorded. Initial pH values ranged from 6.5 to 6.7. 28-days Dissolved oxygen at 0, 5,10, 20, and 28 days. Dissolved oxygen meter calibrated by means of Winkler titration of aerated Milli-Q water each day. 2/28/97 3/28/97 The test generally followed the recommendations of the OECD Guidelines for Testing of Chemicals CALCULATIONS The reported values are based on nominal stock solution loadings at the beginning of the study. The concentrations tested and the corresponding response data derived from the duplicated test are used to calculate, if possible the mg of carbonaceous biochemical oxygen demand per kg of test substance (BOD). The percent biodegradability is then determined by dividing the CBOD by the Chemical Oxygen Demand (COD) and multiplying by one hundred. RESULTS Percent biodegradability values obtained after 5, 10, 20, and 28 days of exposure to P3025 are summarized in Table 1. In duplicated measurements, the percent biodegradability of P3025 was reported as <32% and 44% of chemical oxygen demand. ACCEPTABILITY CRITERIA This biodegradation test meets quality criteria provided in test guidelines: 1) The glucose-glutamic acid reference standard 5-day C-BOD results were within the range normally seen in this laboratory. See Figure 1. 2) The oxygen depletion of the medium blanks was < 0.5 mg/L after 5-days. 3) The oxygen depletion of the medium blanks was < 1.5 after 28-days. 4) The C-BOD result for the toxicity check (highest tested concentration plus glucose-glutamic acid) was greater than the value found for glucose-glutamic acid alone. This indicated the test substance did not inhibit the microorganisms. 5) Only those data points where the corrected dissolved oxygen drop was > 2.0 mg/L and the final dissolved oxygen concentration in the bottle was > 0.5 mg/L were used to calculate C-BOD. C04227 28-Day CBOD Page 4 May 20, 1997 This test was conducted by Trudy K. Stiefenhoefer as requested by Pam Wolf of 3M Specialty Chemicals Division. REFERENCES APHA, AWWA, WEF. 1995. Standard Methods for the Examination of Water and Wastewater. Method 5210 B, 5-Day BOD Test. APHA, AWWA, WEF. 1995. Standard Methods for the Examination of Water and Wastewater. Method 4500-0 C. Oxygen (Dissolved), Azide Modification. OECD. 1993. 301 D Closed Bottle Test. Guidelines for Testing of Chemicals. Organisation for Economic Co operation and Development, Paris, France. OECD. 1981. Principles of Good Laboratory Practice, Annex 2, C(81) 30 (Final): 7-28. OECD Guidelines for Testing of Chemicals. Organisation for Economic Cooperation and Development, Paris, France. Polybac. 1997. Polyseed-NX, "The Original" CBOD Seed Inoculum. Polybac Corporation, Bethlehem, PA. C04228 28-Day CBOD Page 5 May 20, 1997 TABLE 1 SUMMARY OF READY BIODEGRADABILITY OF P3025 ExDosure Period 5-Days Duplicate 10-Days Duplicate 20-Days Duplicate 28-Days Duplicate____________ CBOD, ma/Ka <40,000 <40,000 <40,000 <40,000 <40,000 <40,000 55,039 <40.000 *COD reported as 125,000 mg/kg Percent of COD* <32 <32 <32 <32 <32 <32 44 <32 04229 mg/kg C 04230 28-Day CBOD Page 6 May 20, 1997 FIGURE 1 CBOD GGA 5-day Reference Standard Date PFOS didecyldimethylammonium salt Synonyms: P3025, L-14394 Physical/Chemlcal Properties Title None Laboratory or Date Author Completed Type Environmental Fata and Transport Title Determination of the 28-day Carbonaceous Biochemical Oxygen Demand (CBOD) for P3025 Laboratory or Author 3M Env. Lab Completion Date Type Robust summary, 5/20/97 final report Ecotoxlcity Elements Title Acute Toxicity of P3025 Developmental Material to Fathead Minnow (Pimphates prom elas) Acute Toxicity of P3025 Developmental Material to Daphnia magna Growth Inhibition of P3025 Green Alga (Selenastrum capricom utum ) Growth Inhibition of P3025 to Green Alga (Selenastrum cap rico m u tu m ) Inhibitory Effects of P3025 on Microtox Inhibitory Effects of P3025 on Activated Sludge Respiration Laboratory or Completion Author Date Type AScI Corporation, Robust summary, Duluth, MN 1/2/97 final report AScI Corporation, Robust summary, Duluth, MN 1/10/97 final report AScI Corporation, Robust summary, Duluth, MN 1/29/97 final report AScI Corporation, Duluth, MN 3M Env. Lab 3M Env. Lab 2/21/97 5/12/97 5/12/97 Robust summary, final report Robust summary, final report Robust summary, final report C-04231