Document aBVmqLGwnyL3mGw64dozm34Ee

Younger Laboratories Hiochemliii'. .. Phabmacoloyiili . .. /inali^Ui- 123 CLIFF CAVE ROAD Saint Louis, Mo, 63129 PHONE) TILDEN 6*2540 SUBJSCI Toxicological Investigation Of; ?STD3ACL 62 5 November ?tfc, 1906 iV. fitFSalHiNTAL MOCdOUSi; - A) Oral L030 (Hats, Mixed The undiluted compound weired by st\\cnStfybe to oprague-Davley strain albino Bale and female rats. ( I || V groups of male and 0.1 fractional leg intervals at ffc'fr levels designid**fo blanket the toxicity range thereby supplying da tailor calculation of the LDj0 which was done according to a modification o\\he metho/y&f . J. de Beer. Observation wergNaaqa toxic symptoms and the viscera of the animals tnat ouecumbed were examined macroscopically. The data are shown in Table I. 3) Skin Absorption MLD (Babbits, Mixed Sex) The undiluted compound was applied in increasing doses at increments of 0.2 fractional log intervals to the closely clipped, intact skin, of New oealaad white male and female rabbits. The treated areas were, covered with plastic atrips and the animal5 placed in wocden stocks for periods up to twenty^four hours, after which time they were assigned to individual cages. Observations were made for'toxic symptoms and the viscera of the aninals that succumbed were examined macroscopically. The data are shown in Table II. . 03641V3 Tbi* confidential report it not to be lifted in nny form of ndvettiaing without written pertniawon TOXSTUDIES0728 To) Monsanto Company ' 1< St. Louis, Missouri lounger Laboratories Certificate of Analysis - Page 2 (11/9/66) - Y-66-162 SUMMARY pyPRAPL 62 5 A) Oral LD5o (Rate, Mixed Sex) The Oral LD90 for male and female rats was placed at 20,700 nilllgrams per kilogram with loser and upper limits of 17 590 to 24,630 milligrams per kilogram* The compound as classed as practically non-toxic by oral Ingestion in male and female rats. B) Skin Absorption WLD (Rabbits, Hixed Sex) The Minimum Lethal Dose by Skin Absorption in male and female rabbits was found to be greater than 2000 milligrams per kilogram and less than Jl6o milligrams per kilogram. The compound was classed as slightly toxic by skin absorption in male and female rabbits. XODflGLR LA BORA.TORI id *!*"* is to bo ft -- Mousanto Company 036*l*>`i TOXSTUDIES0729 - Td: Monsanto Company ( ( St. Louis, Missouri lounger Laboratories Certificate of Analysis - Page 5 (11/9/66) - Y-S6-1S2 TAELS I THE ORAL LD50 OF 'PYlK-iAUL 625 FOil i&?3 Sample Fed Undiluted Animal No. - ASex Weight On > 1- Female 2- Male 5- Female 4- Male 5* Female 6- Male 7- Female S- Male 9- Female 10- Male 11" Female 12- Male 15* Female 14- Male 15- Female l6- Kale 17- Female 18- Kale 19* Female 20- Kale 225 250 210 240 215 255 220 245 2x5 250 225 255 220 255 210 260 250 240 215 250 Dose i&Zi& 12,600 12,600 12,600 12,600 12,600 15,000 15,800 15,800 15,000 15,600 20,000 2C,COO 20,000 20,000 20,000 25,100 25,1C0 25,100 25,100 25,100 _____ Fate___ Survived Survived Survived Survived Survived Survived Died Survived Survived Survived Suwiv ed Survived Died Survived Died Survived Died Died Died Died i I The Oral LDS0 for male and female rats was placed at 20,700 milligrams per kilogram with lower and upper limits of 17,590 to 24,6jO milligrams per kilogram. The compound was classed as practically non-toxic by oral ingestion in mile and female rats. Survival time was two to six days. Toxic symptoms Included loss of appetite, diarrhea, increasing weakness, dyspnea, and collapse. At autopsy there was liver, renal, and pulmonary hyperemia. 0364195 TOXSTUDIES0730 ' Toi Monsanto Company V ( St. Louis, Missouri Younger Laboratories Certificate of Analysis - Page 4 (11/9/66) ** Y-66-162 TABLE II THE MINIMUM LETHAL LOSE OF FYDRAUL 62 5* BY SKIN ABSORPTION IN RABBITS Sample Applied Undiluted Lmal Ko. -^Se% 1 - Male 2 * Fercale 5 - Male 4 - Female 5 - Male 6 - Female height JLXi. 2.1 2.5 2.0 2.7 2.S 2.5 . Dose K&ZSfc 734 1260 2000 2160 5cio 7340 height Change 5 Lays Later _____ 5fc_____ + 0.1 *- 0.1 - 0.1 - 0.3 - 0.4 - 0.3 ________ Fata Survived Survived Survived Died ~ 9 Days Died -- 7 Days Died - 5 Lays i El 3SUSSI ON ~ . The Mininun Lethal Dose by Skin Absorption in csale and fersnle rabbits was found to be greater than 2OC0 milligrams per kilogram and less than 31^0 milligrams per kilogram. The compound was classed as slightly toxic by skin absorption in male and female rabbits. Survival time was five to nine days. Toxic symptoms included weakness in two to three days followed by paralysis. At autopsy the lungs were hemorrhagic and there was liver discoloration. 0364196 TOXSTUDIES0731