Document a58oBVJ637edqmQew4vEZnGa
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ACUTE TOXICITY TO FISH
TEST SUBSTANCE
Identity: Perfluorooctanoic acid; may also be referred to as PFOA, FC26, or FX-1001. (Octanoic acid, pentadecafluoro-, CAS # 33567-1)
Remarks: The 3M production lot number was not noted. The test sample is FX-1001. It's purity was not sufficiently characterized, though current information indicates it is a mixture of 95 - 98% test substance and 1 - 5% perfluorochemical inerts.
METHOD:
Method: Not noted.
Type: Static acute
GLP: No
Year completed: 1985
Species: Pimephales promelas
Supplier: Dale Fattig of Brady, Nebraska.
Analytical monitoring: Temperature, pH and DO
Exposure period: 96-hours
Statistical methods: Probit analysis
Test fish age: Not noted.
Length and weight:
Average length = 4.1 cm
Average weight = 0.50 g
Pretreatment: 25.0 mg/L tetracycline HCI, 5 months prior to study
initiation.
Test conditions: Dilution water: Carbon-filtered well water. Dilution water chemistry at test initiation: Temperature: 19C Dissolved Oxygen: 9.5 ppm pH: 7.8
Stock and test solution preparation: A stock solution was prepared by dissolving 45 g test substance (neutralized with NaOH to pH 7.5) in 3 liters of water and transferring appropriate aliquots to test vessels to make 5 liters of test solution at the selected test concentrations. Concentrations dosing rate: Once Stability of the test chemical solutions: Not noted Exposure vessels: 24 cm inside diameter and 26 cm deep glass beakers containing 5 liters test solution. Number of replicates: two Number offish per replicate: 5
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Loading rate: 0.50 g/L Number of concentrations: five plus a blank control Water chemistry during the study:
Dissolved oxygen range (24 - 96 hours): 7.3 - 7.7 mg/L (control exposure) 6.1 - 6.7 mg/L (870 mg/L exposure)*
pH range (24 - 96 hours): 7.5 - 7.7 (control exposure) 7.5 - 7.5 (870 mg/L exposure)*
* 870 mg/L test group (second highest concentration) data given because total mortality occurred in the highest test concentration by
48 hours. Test temperature (24 - 96 hours): 19 - 20C
RESULTS
________________________________
Nominal concentrations: Bk control, 690, 750, 810, 870, and 930 mg/L Element value: 96-hour LC50 = 843 (811 - 878) mg/L
Element value based on nominal concentrations
CONCLUSIONS
The test sample 96-hour LC50 for fathead minnow was determined to be 843 mg/L with a 95% Confidence Interval of 811 to 878 mg/L.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY
_____
_________
Reliability: Klimisch ranking 2. Testing meets the criteria for quality testing. However, sample purity was not properly characterized and it lacks analytical confirmation of test substance concentrations.
REFERENCES
This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request Number C1006, completed on February 2,1985.
OTHER
Last changed: 5/24/00
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ENVIRONMENTAL LABORATORY
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TABLE I. SUMMARY OF ACUTE TOXICITY OP E -X /n r> {
TO FATHEAD MINNOW UNDER ~ S ^y -z f7 7 f^______ EXPOSURE CONDITIONS^1^
CHEMICAL CONCENTRATION (ag/1)
24-HR.
PERCENT MORTALITY 48-HR.
72-HR.
96-HR.
CONTROL Mo *fc>o .61 n
- fftt? 4.*o
o o 1o 10 10 <40
o lO O >40 ICO
c O \0 JO 4C IGO
o >
10 ^> o jS O toe
LC.n (9SZ CONFIDENCE LIMITS)(2)
50 ag/1
>4*0
84q<j6n-66'l)
644(614-664)
8*5(6"'
(1) Decs ere average of two replication for each concentration with
fish per
replicate. A total of twenty fish were used per concentration. ten .
(2) LC^q values were calculated using logistic regression aethods (Probit Analysis).
(3 ) Nicte. -{ke d c s e /re 5poiLS^ Q ^O v ^lJ t jZ X ffo H otf4aM ^y
Odttitc
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/ ` kW M r k # w
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DE:Fi:ti::TiVE :full- scale t ;::s t )
=vMBOMKECtU-w Li4*
-`la te rU l le s a id _ i
LR rt i.. Lot
'oat Organigg f e ^ ^ r / y > / ^ Som ce^ ? ^ y f ^ a t ^ W M ^ ^ ^ / ^ B a t c h I r& s / r& )
ivg.Wt^ ?/, V ) grace Avg. Size / camNo*_teganisms p er dose "rf~' Loading Factor / ? , ,'SV"J g /1
'ish Disease Treatment
Pest S o lu tio n Voinne ^ 'S " L ita te /A ll-G la s s tin s S ta rte d > < / St ar t ed D ilu tio n Water c a u to n -filtg o a d w e ll w ater <Tenp.
, w* d<rtP deep ^
Date Ccepleted Zyf/B Zf
C ., D.O. ^*5 T p p n , pH
f
Investigai (Full Signal
(95% Confidence Lim its)
Tank I n it ia l T o ta l 1 24 Hours
48 Hours
72 Hours
96 Hours
|
No. Gone. Amount T o ta l Ho. PB D.O. T o ta l No. pH D.O. T o ta l No. pH D.O. T o ta l No. pH D.O.
mg/1 Added Dead
PE* Dead
PE*" Dead
PE*" Dead
ppn
X Cbntxo]
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<?p{j o 74 (/y o l o 7,0 O S
i-7 ? y< r< ? r? 5 T ?
lr ^ > P)in 'f W t i k e y e pf)n m ; 9s*n >ta
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y
l. fai - l 2 /
7'3 '/t/ ?> i Lhf _ ^ r
tu w - o Ul - /
l 1, fait -f)
S Y/) 0 ,
Y,!
te If,Y / 22 /.9
t e (fifi J Z - 22
v /T
T est Temo. C In itia ls & Date
V ^ /J T W iS m T /F J X r^
i v rm
Secondary
~ ^ U U iS ,'S o ff` t-
iO + 's '
Tank No. Synptcnvs o r R eactions
Control lL
H
I-O .
24-H r.
T o tal Nrdber A ffected
48-Hr.
72-Hr.
/. . i. . r ^ ...
/
- ^ U ^ i T / P X m 4-.
12 > 1
/ --
yT 1
96-Hr.
. j c c f f - ...........V'
/ _________ C Q 3 8 S 2
AOVATIC TOXICITY DATA SHEET DEFINITIVE (FULL-SCALE TEST)
jrtwwwnw- wiB ype ^ 4 /s M r ) 4 * A ^ L ^ Z g M a t e r i a l T ested
LR
t o t ---------------------------------
e s t ( ^ a n i ^ ^ ^ y / ^ / ^ ^ S o u rje ^ ^ ^ ^ ^ - D a te R e e Bat ch
vwg.Wt.
grams Avg. Size / a n No. Chpgea^ians per dose
Loading Eactor_
q /1
ish D isease Treatm ent: . U < Y , r ) a
J: f
{ _^
X3>. a . <3-5~guA. d<rfip
est Solution Volane^^ Liters/All-Glass Tank Tank Dimensions: 9Djl20 urani 25
ime S ta rte d
Date S tarted
XU * t e Ocmpleted
ilu tio n Water C arb o n -filtered w ell w ater (Tenp. / t f C ., D.O. ^T" ppm, pH % f i ' ' )
LCgQ (95% Confidence Lim its)/^_2<T/VV /--
Tank I n i t i a l T otal | 24 Hours
48 Hours
72 Hours
96 Hours
|
No. Gone. Amount T otal No. pH D.O. T otal No. pH D.O. T o tal No. pa D.O. T o tal No. P D.O.
iog/1 Added Dead
ppn Dead
ppn Dead
ppn Dead
ppn
w x ~ Oontrol
Z1 77 O ZL fis a Ik A f) < 9 Is 7<
Z .-I /r fQ 1 Y .S O
o T&
1 2k
o ... Ujl Y fQ ,
/ Ul M
n
/
l l
C
25 l )
( 25 Y i
l f i / O pY /
2-4 ffY y ? 0
Zk >$ Z b i
tk 6 fi 7
2 j l
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l ?f) f t
l ? ,S
9 *fi
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,s T
i
LT est Tene. C_____
.~ 2 c * ____ i
____ V ~ W S L z___ _____
oondary
Tank No. Synjtoms o r R eactions o n tro l JT 2 .-1 2 --?
^ -4
24-Hr. /
T o tal Number A ffected
48-Hr.
72-Hr.
/
.. \V J . .. .
U
.
/V
............
//
y T-
96-Hr.
U ry
y
t-u u o ao
