Document a4wKxJp5kvgbgGqwaq224bEXM

R J REPORT % AR226-2753 Copies Material Tested Uctanoic acid, pentadecafluoro-, ammonium sait**i E. I. du Pont de Nemours and Company Haskell Laboratory for Toxicology and industrial Medicine Elkton Road, Newark, Delaware^ 19711 HASKELL LABORATORY REPORT NO. 659-79 Haskell Other Study Initiated/ ______ Completed ____ 8/21-9/12/79 (rabbits) 8/22-9/11/79 (rats) p ______ Material Submitted by _________ I J i ^ ^ H M P l a s t i c Products and Resins DepartEen^Twashington Works Procedure: (Rabbits) SKIM ABSORPTION LD50 - RABBIT AND RAT i Fouy BrouPs f raale albino rabbits were clipped free of hair over the back and, trunk area and equipped with F ^ co ars" T e proper dose of test material (made into a slurry with water) was applied to the back of each rabbit and wrapped successively with layers of Saran Wrap, KlinjP gauze bandages and Elastoplast elastic bandages. After 24 Hours ot exposure, the animals were unwrapped* washed with water, dried and returned to cages for 14 or 15 days obser on or until death. During the observation period, the animals were weighed and observed for clinical signs. Gross pathology was done on all survivors and 1 animal that was found dead at 3,000 mg/kg. The LD50 values were calculated rium the mortality data using the method of D. J. Kinney.t Procedure : (Rats) Three groups of ChR-CD male vats 5 rats per group, were clipped free of hair over the back and trurik area. The proper dose of test material , as a 50/50 aqueous suspension, was applied to the back of each rat under a Square of a iinunum foil and held in place with 3 Elastoplast . After 24 hours exposure, the rats were unwrapped, sponged off with a mi detergent, rinsed, dried and returned to cages for 13 days observation or until death. During the observation period, the rats were weighed and observed for clinical signs. Gross pathology was done on all survivors and l rat that uled at /,500 mg/kg. The LD50 values were calculated from the mortality data using the method of D. J. Finney.t Composition I Finney, D. J., Probit Analysis. 3nd Ed. 197.1, Cambridge University Press . (Hata with MR file) due rat dosed at /, 500 mg/kg did not have liver weight recorded at: pathology. Company Sanitized. Does not contain T S C A CBI Resu'L cs : Lj . of AnLmaLs 2 5 5 5 (Rabbles) Dose fog/kg) Average Dose (g) (Aqueous Slurry) Average Weight Initial/Final Mortality1 _________________ Clinical S i g n s _________ 7,500 5,000 3,000 1,500 25.28 16.63 10.39 4.99 3370.5/ 3326 / 2872 3463.2/2827.2 3320 / 3002 ID - 2 d ID - 3 d 2D - 3 d ID - 4 d 2S - 14 d ID - 7 d 2S - 14 d 2S - 15 d 5S - 14 d Weight>loss, labored breaching, cyanosis, body wet underneath and Moderate skin irritation (erythema). Nasal discharge,! shallow breathing, pallor, diar rhea, wet underneath body, weakness, weight loss and skin irritation with necrotic spots. Labored breathing, lethargy, skin irritation and weight loss. Labored breathing, skin irritation with rusty hard area and weight loss. LD50 = 4,278 mg/kg 95% Confidence Limits: Lower: 2,369 mg/kg Upper: 9,814 mg/kg Slope: 6.2504 Results: No. of 'Animals (Rats) Dose (mg/kg) 5 7,50L 5 5,000 5 i,000 Average Dose (g) Average Weight (Aqueous Slurry) Initial/Final Mortality^ Clinical Signs 1.9 Gross Pathology: 254 / 298 3D - Id. ID - 2 d. IS - 14 d. Lethargy, chromo^lacryorrhea, stained nose, wet and stained perineali area and initial weight loss. 2 rats: 1 FD at 1 day - liver weight not taken. No other compound-related signs noted. 1 Survivor (14 days) - increase in liver weight. 1.4 276 / 316 5S - 14 d. Lethargy, wet perineal area and initial weight loss Gross Pathology: 5/5 Rats: Increase in liver weight. 0.87 290 / 345 5S - 14 d. Initial weight loss only. Gross Pathology: 5/5 Rats: Increase in liver weight. LD50 - 6,959 mg/kg 95% Confidence Limits not defined - Slope: 25.9 Company Sanitized. Does net contain T S C A CBl 3 Summary: (Kabbits) Oiiunoic acid, pentaducafluoro-, ammonium saLt is moderately toxic by skin absorption in rabbits* its ID50 is 4,278 mg/kg of body i^ight. ' Gross patbologi.i.al examination showed no compound-related changes; however, there was skin IrriLation at all levels. Clinical signs included weight loss and labored breathing at all levels and wet underneath the body, cyanosis diarrhea and lethargy appearing at mos' levels. ' r'! Summary; (Rats) Octanoic acid, pentadecafluoro-, ammonium salt is slightly toxic in male rats by skin absorption; its LD50 is 6,959 mg/kg of body weight. All rats dosed at 7,500, 5,000 and 3,0UQ mg/kg had initial weight loss. Rats dosed at 7,500 and 5,000 mg/kg were lethargic on the day of treatment and the majority had wet and/or stained perineal areas. Chromodacryorrhea was seen in rats treated at 7,500 mg/kg. Gross pathology showed an increase in liver weights in all surviving rats examined at 14 days after treatment. DFE/JEH:scg Report No. 659-79 Date Issued: October 26, 1979, a,e Reissued: February 26, 198(7 Repor t by: fl* ' ^ <" Doris F. Edwards Toxicologist i 1fctvw. .<___ John E. Henry Technician Approved by: Gerald L. Kennedy Chief, Acute Investigations Section Company Sanitized. Does not contain T SC A CBI