Document a4d0wkb015X2dyVan8688VE5M
UNITED STATES ENVIRONMENTAL PROTECTION AGENCY REGION 1
Laboratory Services and Applied Science Division 11 Technology Drive
North Chelmsford, Massachusetts 01863-2431
Drafted Date: Finalized Date:
08/26/2022 10/6/2022
Subject:
Reconnaissance and Sampling Inspection Report Clean Water Act - National Pollutant Discharge Elimination System City of Methuen, MA
From:
Michelle Coombs, Inspector Laboratory Services and Applied Science Division (LSASD) Field Services Branch (FSB)
/MRC/
Reviewed By:
Jerome Keefe, Investigations Team Leader, LSASD-FSB
/JCK/
To:
Denny Dart, Water Compliance Section Chief,
Enforcement and Compliance Assurance Division (ECAD-EWC)
CC:
Elizabeth Kudarauskas, Water Technical Lead, ECAD-EWC
I. Facility Information
A. Facility Name: City of Methuen, MA B. Facility Location: 41 Pleasant St, Searles Bldg. Room 206, Methuen, MA 01844 C. Facility Contact: Stephen Gagnon, Engineering Dept. Administrator,
SJGagnon@ci.methuen.ma.us, (978) 983-8550 D. NPDES Permit ID:MA041210 (MS4)
II. Background Information
A. Date/Start time of recon inspection: 08/24/2022, 11:15 AM B. Weather conditions: Mostly cloudy, ~70F C. USEPA Representatives: Shannon Brunelle, Michelle Coombs D. State/Local Representatives: None A. Date/Start time of sampling inspection: 09/06/2022, 10:00 AM B. Weather conditions: Overcast, rain, ~70F C. USEPA Representatives: Michelle Coombs and Caitlin Brown (LSASD) D. State/Local Representatives: None E. Federally Enforceable Requirements Investigated:
Stormwater discharges and certain non-stormwater discharges from the City of Methuen, MA (the City's) Municipal Separate Storm Sewer Systems (MS4) are authorized by the National Pollutant Discharge Elimination System (NPDES) General Permit for the Discharge of Stormwater from Small MS4 (hereinafter, the Permit), issued by the Environmental Protection Agency (EPA) Region 1. The City of Methuen obtained coverage under the Permit with NPDES Permit ID MA041210.
City of Methuen, MA (MA041210) EPA MS4 Compliance Sampling Inspection
F. Pollutants Sampled: Pharmaceuticals and Personal Care Products (PPCPs), E. coli/Enterococcus, Ammonia (nitrogen), Total Chlorine, and Surfactants
III. Disclaimer:
Unless otherwise noted, this report describes conditions at the facility/property as observed by EPA inspector(s), and/or through records provided to and/or information reported to EPA inspector(s) by facility representatives and as understood by the inspector(s). This report may not capture all operations or activities ongoing at the time of the inspection. This report does not make final determinations on potential areas of concern. Nothing in this report affects EPA's authorities under federal statutes and regulations to pursue further investigation or action.
IV. Type and Purpose of Inspection(s)
The purpose of the reconnaissance inspection was to evaluate access requirements of various locations along Bloody Brook, while noting appearance and presence of flow for one or more sampling inspections.
The purpose of the sampling inspection was to identify potential illicit connections or illegal discharges within the City of Methuen, MA MS4 that may adversely impact the water quality in Bloody Brook. Samples were collected from eight (8) locations in accordance with the FSB Investigations Team Stormwater Program Plan. Sample sites were identified during a reconnaissance inspection conducted on 08/24/2022.
V. Permittee Details
The City of Methuen, MA occupies approximately 23 square miles in Essex County, MA, approximately 30 miles northwest of Boston, MA. The City of Methuen shares borders with the municipalities of Haverhill to the northeast, North Andover to the east, Lawrence and Andover to the south, Dracut (Middlesex County) to the west, Pelham, New Hampshire (Hillsborough County) to the northwest, and Salem, New Hampshire (Rockingham County) to the north. According to the 2020 U.S. Census, the regulated population of Methuen was 53,059. Bloody Brook is listed as an MS4 receiving water in the City of Methuen Notice of Intent (NOI) for coverage under the permit.
VI. 08/24/2022 Reconnaissance Inspection Summary
On Wednesday, August 24, 2022, EPA employees Shannon Brunelle and Michelle Coombs ("the EPA Inspection Team") conducted an unannounced reconnaissance inspection of the City of Methuen MS4. They were not accompanied by any city or state representatives. The inspection started at approximately 11:15 AM where Bloody Brook flows underground at the CVS on Jackson St. At the time of the inspection, the weather was approximately 70F with mostly cloudy skies.
According to the National Weather Service (NWS) for the Lawrence Municipal Airport (KLWM), the last precipitation event prior to the inspection occurred on August 23, 2022, with 0.05 inches recorded. There was 0.27 inches precipitation recorded within the 72 hours before the inspection. In the month of August (to date), the area observed 0.52 inches of rain, which is
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Sampling Inspection Date: 09/06/2022
City of Methuen, MA (MA041210) EPA MS4 Compliance Sampling Inspection
approximately 22% of the 30-year normal value of 2.38 inches. According to the US Drought Monitor, as of 08/30/2022, Essex County was experiencing extreme drought conditions.
None of the locations visited during this inspection were field screened with the test kits or the YSI quality meter. Locations visited were noted for appearance, presence of flow, and access requirements. Photographs taken during the inspection are listed in Appendix A.
VII. 09/06/2022 Sampling Inspection Summary
On Tuesday, September 6, 2022, EPA employees Michelle Coombs and Caitlin Brown ("the EPA Inspection Team") conducted an unannounced compliance sampling inspection of the City of Methuen, MA MS4 at the locations described in Section VIII. They were not accompanied by any city or state representatives. The inspection started at approximately 10 AM where Bloody Brook flows underground at the CVS on Jackson St. At the time of the inspection, the weather was approximately 70F and raining with overcast skies.
According to the NWS for the KLWM, the last precipitation event prior to the inspection occurred on September 5, 2022, with 1.25 inches recorded. In the month of September (to date), 1.85 inches of rain was observed, which is approximately 264% normal of the 30-year normal value of 0.70 inches. According to the US Drought Monitor, as of September 6, 2022, Essex County is experiencing severe to extreme drought conditions.
The sampling locations described in Section VIII were not field screened with the test kits for ammonia (nitrogen), surfactants, and total chlorine due to high flow conditions and a strong dilution factor potential, with the exception of "BrookOut". In-situ measurements were taken using a YSI meter for specific conductivity, salinity, and temperature at all locations. All field data were recorded on field data sheets. Samples were delivered to and analyzed for E. coli and Enterococcus and PPCPs at the EPA New England Regional Laboratory (NERL) in North Chelmsford, MA. Table 1 below summarizes the sampling and analytical data.
VIII. Description of Sampling Locations (in order of collection time) Photographs depicting each sample location are included in Appendix A of this report.
CVS: An in-stream sample of Bloody Brook collected near the CVS parking lot at the corner of Jackson Street and Swan Street. Some trash and duckweed was observed floating on the stream surface. Sample was collected from the stream prior to the reinforced concrete inflow pipe that flows under the parking lot. The outfall adjacent to this inflow point was dry and not flowing.
Curtis: An in-stream sample of Bloody Brook collected at a single steel ovular stream culvert approximately 30 inches in diameter located on Curtis Street near the intersection with Bicknell Avenue. Discharge flow was approximately 50 gallons per minute (gpm) and is Bloody Brook upstream of "CVS". Sample was collected at the mouth of the culvert. A smaller concrete outfall to the left of the culvert was flowing, and upon further examination, the flow was coming from a catch basin nearby on Curtis Street.
47Bick: An in-stream sample of Bloody Brook collected at a single reinforced concrete stream culvert approximately 24 inches in diameter located at 47 Bicknell Street where Bloody Brook daylights from underneath Bicknell Avenue. Stream flow was
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approximately 15 gpm and is an upstream location of "Curtis". Sample was collected at the mouth of the culvert. 96East: An in-stream sample of Bloody Brook collected at a concrete stream culvert where the brook flows underneath East Street near the intersection with Jackson Street. Outfall characteristics could not be determined due to thick vegetation overgrowth over the culvert. Sample was collected at the base of the vegetation, approximately 6 inches from the mouth of the culvert. BrookOut: A single concrete outfall approximately 15 inches in diameter located adjacent to 180 Jackson Street. Discharge flow was approximately 5-7 gpm into Bloody Brook. Sample was collected at the mouth of the outfall. Brook: An in-stream sample of Bloody Brook collected at a rectangular stream culvert that directs flow underneath Brook Street near the intersection of Jackson Street. Sample was collected at the mouth of the culvert. Trash was observed downstream along both banks. Cedar: A single white PVC pipe approximately 24 inches in diameter located on the south side of the Cedar View Nursing Home. Discharge flow was approximately 12-15 gpm. A slight musty odor was detected. Sample was collected at the mouth of the outfall. Dearborn: An in-stream sample of Bloody Brook collected at a singular steel stream culvert approximately 48 inches in diameter where the brook flows underneath Dearborn Road. Discharge flow was approximately 50 gpm. Some trash was observed downstream and along the banks. Orange staining of the sediment was observed, and a slight musty odor was detected. Sample was collected at the mouth of the culvert.
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City of Methuen, MA (MA041210) EPA MS4 Compliance Sampling Inspection
Map of Sample Locations
Note: the locations on the map were estimated using Google Earth and are used for display purposes only.
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Table 1: Summary of Sampling and Analytical Data
Site ID
CVS
Curtis
47Bick
96East BrookOut Brook
Cedar Dearborn
Location
Sample Date
9/6/22
9/6/22
9/6/22
9/6/22
9/6/22
9/6/22
9/6/22
9/6/22
Sample Time
10:00
10:14
10:31
10:46
11:11
11:13
11:43
11:58
Coordinates1 (Decimal degrees)
North West
42.723491 -71.158446
42.723956 -71.158704
42.725974 -71.160000
42.726326 -71.162087
42.729698 -71.164418
42.729732 -71.164377
42.733045 -71.166526
42.735490 -71.168122
Salinity, ppt.
0.2
0.2
0.2
0.3
0.0
0.3
0.1
0.1
YSI Meter Temperature, C
19.1
19.2
19.2
19.1
19.6
19.3
19.2
19.6
Conductivity, S/cm
391.5
437
453.2
527
25
600
120.2
112.5
Field Test Kits (mg/L)
Ammonia Total Chlorine Surfactants
N/A
N/A
N/A
N/A
0.00
N/A
N/A
N/A
N/A
N/A
N/A
N/A
0.00
N/A
N/A
N/A
N/A
N/A
N/A
N/A
0.25
N/A
N/A
N/A
Bacteria (MPN/100mL)
E. coli Enterococcus
3,080 8,164
2,908 19,863
9,678 12,997
3,080 5,172
689 3,654
2,190 1,317
3,683 12,033
3,683 8,664
Cotinine
19
17
18
16
13
19
26
32
Acetaminophen
11
9.0
13
8.9
13
10
6.9
10
Paraxanthine
22
14
14
14
7.7
20
7.1
8.6
PPCPs (ng/L)
Atenolol Caffeine
ND
ND
ND
ND
ND
ND
ND
ND
180
130
210
120
55
130
160
200
Metoprolol
ND
ND
ND
ND
ND
ND
ND
ND
Diphenhydramine
ND
ND
ND
ND
ND
ND
ND
ND
Carbamazepine
ND
ND
ND
ND
ND
ND
ND
ND
Abbreviations and Notes:
1. The coordinates listed in Table 1 were derived from the map created in Google Earth, therefore the coordinates are estimates of the actual locations.
N/A: Not Analyzed
ND: Not Detected above Reporting Limit
E. Coli: Red 10,000 col/100ml, Orange 1,260 col/100ml, Yellow 410 col/100ml, Black < 410 col/100ml
Entero: Red 1,000 col/100ml, Orange 350 Yellow 130 col/100ml, Black < 130 col/100ml
NH3: Red 6 mg/L, Orange 0.5 mg/L, Yellow 0.25 mg/L, Black < 0.25 mg/L
Cl2: Red 1.0 mg/L, Orange 0.3 mg/L, Yellow 0.02 mg/L, Black < 0.02 mg/L
Surfactants: Red 1.0 mg/L, Orange 0.5 mg/L, Yellow 0.25 mg/L, Black < 0.25 mg/L (may give false positive at salinity greater than 1 ppt)
PPCPs: Dark Pink 100x the RL; Pink 10x the RL; Light Pink 3x the RL; No Pink < 3x the RL*
*See Reporting Limit (RL) values for each compound in attached Laboratory Report for Source Tracking
EPA notes while there are currently no numerical standards to compare pharmaceutical results against, it is EPA's experience that acetaminophen is the single best bacterial source
tracking compound of those listed above, and any detection of this compound may indicate a source of sanitary sewage. With respect to all of the above compounds, when a sanitary sewage
source is present, depending on the type of source, distance from the sample location, and the strength of the source, concentrations of these compounds may range from the low ng/l range
up to thousands of ng/L.
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IX. Appendix Appendix A: Photograph Log X. Attachments Attachment A: Laboratory Report for PPCPs (Source Tracking) Analysis Attachment B: Laboratory Report for E. coli Analysis Attachment C: Laboratory Report for Enterococcus Analysis
End of Report
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Appendix A Photograph Log
Photo Name CVS Curtis 47Bick 96East
BrookOut Brook Cedar
Dearborn
Time 10:04 10:19 10:34 10:49 11:10 11:10 11:43 11:59
Date 9/6/22 9/6/22 9/6/22 9/6/22 9/6/22 9/6/22 9/6/22 9/6/22
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CVS: In-stream sample of Bloody Brook collected just before the opening of the culvert pipe that flows under the parking lot. Flow direction indicated by a blue arrow. Sample
point is indicated by a red "X".
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Curtis: In-stream sample of Bloody Brook collected at the stream culvert on Curtis St. Flow direction indicated by a blue arrow. Sample point is indicated by a red "X". The
smaller flowing outfall is indicated by a red circle.
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47Bick: In-stream sample of Bloody Brook at approximately 47 Bicknell Street. Flow direction indicated by a blue arrow. Sample point indicated by a red "X".
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96East: In-stream sample of Bloody Brook at the stream culvert in the parking lot of 96 East Street. Flow direction indicated by a blue arrow. Sample point indicated by a red
"X".
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BrookOut: A singular concrete outfall, flowing into Bloody Brook on Brook Street. Flow direction is indicated by a blue arrow. Sample point is indicated by a red "X".
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Brook: In-stream sample of Bloody Brook at the stream culvert on Brook St. Note "BrookOut" is located on the left side of the photo (red circle). Flow direction is
indicated by a blue arrow. Sample point is indicated by a red "X".
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Cedar: A white PVC outfall located on the south side of the Cedar View Nursing Home. Flow direction indicated by a blue arrow. Sample point indicated by a red "X".
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Dearborn: An in-stream sample of Bloody Brook collected at the stream culvert on Dearborn Road. Flow direction indicated by a blue arrow. Sample point indicated by a
red "X".
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Attachment A Laboratory Report for Source Tracking Analysis Author: LSASD Laboratory Services Branch (LSB)
Pages: 14
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September 15, 2022
Michelle Coombs - LFSB US EPA New England R1
United States Environmental Protection Agency Laboratory Services and Applied Science Division
11 Technology Drive North Chelmsford, MA 01863-2431
Laboratory Report
Page 1 of 14
Project Number: 22090004 Project: MS4 Methuen, MA Analysis: HPLC/MS/MS Source Tracking Analysis EPA Chemist: Peter Philbrook
Date Samples Received by the Laboratory: 09/06/2022 Analytical Procedure:
All samples were received and logged in by the laboratory according to the USEPA New England Laboratory SOP for Sample Log-in. Sample preparation and analysis was done following the EPA Region I SOP, LSBSOP-LCMS_STA3.
The SOP is based on an EPA Regional Analytical Method developed at the EPA New England Laboratory.
Data were reviewed in accordance with the internal verification procedures described in the EPA New England Quality Manual for NERL. Results relate only to the items tested or to the samples as received by the Laboratory. This analytical report shall not be reproduced except in full, without written approval of the laboratory.
If you have any questions please call me at 617-918-8340 . Sincerely,
22090004$STA
Qualifiers:
RL = Reporting limit ND = Not Detected above Reporting limit NA = Not Applicable due to high sample dilutions or sample interferences NC = Not calculated since analyte concentration is ND. J = Estimated value J1 = Estimated value due to MS recovery outside accceptance criteria J2 = Estimated value due to LFB result outside acceptance criteria J3 = Estimated value due to RPD result outside acceptance criteria J4 = Estimated value due to LCS result outside acceptance criteria E = Estimated value exceeds the calibration range L = Estimated value is below the calibration range B = Analyte is associated with the lab blank or trip blank contamination. Values are
qualified when the observed concentration of the contamination in the sample extract is less than 10 times the concentration in the blank. R = No recovery was calculated since the analyte concentration is greater than four times the spike level. P = The confirmation value exceeded 35% difference and is less than 100%. The lower value is reported. C = The identification has been confirmed by GC/MS. A = Suspected Aldol condensation product. N = Tentatively identified compound.
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22090004$STA
U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH MS4 Methuen, MA
HPLC/MS/MS Source Tracking Analysis
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Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis:
Dry Weight Prepared: Wet Weight Prepared:
CVS 9/06/2022 9/07/2022 9/12/2022 N/A N/A
CAS Number
486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4
Compound
Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine
Concentration ng/L
19 11 22 ND 180 ND ND ND
Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH:
AC01526 Stormwater 500 mL N/A 1 N/A
Dilution
1 1 1 1 1 1 1 1
RL ng/L
Qualifier
0.40
2.0
2.0
2.0
4.0
2.0
0.40
0.40
Surrogate Compounds Phentermine-D5 Diphenhydramine-D3
Recoveries (%) 74 52
QC Ranges 37 - 113 8 - 114
Comments:
22090004$STA
U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH MS4 Methuen, MA
HPLC/MS/MS Source Tracking Analysis
Page 4 of 14
Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis:
Dry Weight Prepared: Wet Weight Prepared:
CURTIS 9/06/2022 9/07/2022 9/12/2022 N/A N/A
CAS Number
486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4
Compound
Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine
Concentration ng/L
17 9.0 14 ND 130 ND ND ND
Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH:
AC01527 Stormwater 500 mL N/A 1 N/A
Dilution
1 1 1 1 1 1 1 1
RL ng/L
Qualifier
0.40
2.0
2.0
2.0
4.0
2.0
0.40
0.40
Surrogate Compounds Phentermine-D5 Diphenhydramine-D3
Recoveries (%) 76 48
QC Ranges 37 - 113 8 - 114
Comments:
22090004$STA
U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH MS4 Methuen, MA
HPLC/MS/MS Source Tracking Analysis
Page 5 of 14
Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis:
Dry Weight Prepared: Wet Weight Prepared:
47BICK 9/06/2022 9/07/2022 9/12/2022 N/A N/A
CAS Number
486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4
Compound
Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine
Concentration ng/L
18 13 14 ND 210 ND ND ND
Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH:
AC01528 Stormwater 500 mL N/A 1 N/A
Dilution
1 1 1 1 1 1 1 1
RL ng/L
Qualifier
0.40
2.0
2.0
2.0
4.0
2.0
0.40
0.40
Surrogate Compounds Phentermine-D5 Diphenhydramine-D3
Recoveries (%) 72 46
QC Ranges 37 - 113 8 - 114
Comments:
22090004$STA
U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH MS4 Methuen, MA
HPLC/MS/MS Source Tracking Analysis
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Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis:
Dry Weight Prepared: Wet Weight Prepared:
96EAST 9/06/2022 9/07/2022 9/12/2022 N/A N/A
CAS Number
486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4
Compound
Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine
Concentration ng/L
16 8.9 14 ND 120 ND ND ND
Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH:
AC01529 Stormwater 500 mL N/A 1 N/A
Dilution
1 1 1 1 1 1 1 1
RL ng/L
Qualifier
0.40
2.0
2.0
2.0
4.0
2.0
0.40
0.40
Surrogate Compounds Phentermine-D5 Diphenhydramine-D3
Recoveries (%) 77 52
QC Ranges 37 - 113 8 - 114
Comments:
22090004$STA
U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH MS4 Methuen, MA
HPLC/MS/MS Source Tracking Analysis
Page 7 of 14
Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis:
Dry Weight Prepared: Wet Weight Prepared:
BROOKOUT 9/06/2022 9/07/2022 9/12/2022 N/A N/A
CAS Number
486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4
Compound
Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine
Concentration ng/L
13 13 7.7 ND 55 ND ND ND
Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH:
AC01530 Stormwater 500 mL N/A 1 N/A
Dilution
1 1 1 1 1 1 1 1
RL ng/L
Qualifier
0.40
2.0
2.0
2.0
4.0
2.0
0.40
0.40
Surrogate Compounds Phentermine-D5 Diphenhydramine-D3
Recoveries (%) 78 30
QC Ranges 37 - 113 8 - 114
Comments:
22090004$STA
U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH MS4 Methuen, MA
HPLC/MS/MS Source Tracking Analysis
Page 8 of 14
Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis:
Dry Weight Prepared: Wet Weight Prepared:
BROOK 9/06/2022 9/07/2022 9/12/2022 N/A N/A
CAS Number
486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4
Compound
Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine
Concentration ng/L
19 10 20 ND 130 ND ND ND
Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH:
AC01531 Stormwater 500 mL N/A 1 N/A
Dilution
1 1 1 1 1 1 1 1
RL ng/L
Qualifier
0.40
2.0
2.0
2.0
4.0
2.0
0.40
0.40
Surrogate Compounds Phentermine-D5 Diphenhydramine-D3
Recoveries (%) 70 45
QC Ranges 37 - 113 8 - 114
Comments:
22090004$STA
U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH MS4 Methuen, MA
HPLC/MS/MS Source Tracking Analysis
Page 9 of 14
Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis:
Dry Weight Prepared: Wet Weight Prepared:
CEDAR 9/06/2022 9/07/2022 9/12/2022 N/A N/A
CAS Number
486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4
Compound
Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine
Concentration ng/L
26 6.9 7.1 ND 160 ND ND ND
Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH:
AC01532 Stormwater 500 mL N/A 1 N/A
Dilution
1 1 1 1 1 1 1 1
RL ng/L
Qualifier
0.40
2.0
2.0
2.0
4.0
2.0
0.40
0.40
Surrogate Compounds Phentermine-D5 Diphenhydramine-D3
Recoveries (%) 74 45
QC Ranges 37 - 113 8 - 114
Comments:
22090004$STA
U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH MS4 Methuen, MA
HPLC/MS/MS Source Tracking Analysis
Page 10 of 14
Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis:
Dry Weight Prepared: Wet Weight Prepared:
DEARBORN 9/06/2022 9/07/2022 9/12/2022 N/A N/A
CAS Number
486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4
Compound
Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine
Concentration ng/L
32 10 8.6 ND 200 ND ND ND
Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH:
AC01533 Stormwater 500 mL N/A 1 N/A
Dilution
1 1 1 1 1 1 1 1
RL ng/L
Qualifier
0.40
2.0
2.0
2.0
4.0
2.0
0.40
0.40
Surrogate Compounds Phentermine-D5 Diphenhydramine-D3
Recoveries (%) 70 44
QC Ranges 37 - 113 8 - 114
Comments:
22090004$STA
U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH MS4 Methuen, MA
Laboratory Blank
Page 11 of 14
Client Sample ID: Date of Collection: Date of Preparation: Date of Analysis:
Dry Weight Prepared: Wet Weight Prepared:
N/A N/A 9/07/2022 9/12/2022 N/A N/A
CAS Number
486-56-6 103-90-2 611-59-6 29122-68-7 58-08-2 56392-17-7 58-73-1 298-46-4
Compound
Cotinine Acetaminophen Paraxanthine Atenolol Caffeine Metoprolol Diphenhydramine Carbamazepine
Concentration ng/L
ND ND ND ND ND ND ND ND
Lab Sample ID: Matrix: Amount Prepared: Percent Solids: Extract Dilution: pH:
N/A Stormwater 500 mL N/A 1 N/A
Dilution
1 1 1 1 1 1 1 1
RL ng/L
Qualifier
0.40
2.0
2.0
2.0
4.0
2.0
0.40
0.40
Surrogate Compounds Phentermine-D5 Diphenhydramine-D3
Recoveries (%) 83 100
QC Ranges 37 - 113 8 - 114
Comments:
22090004$STA
PARAMETER
Acetaminophen Atenolol Caffeine Carbamazepine Cotinine Diphenhydramine Metoprolol Paraxanthine
U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH MS4 Methuen, MA
MATRIX SPIKE (MS) RECOVERY
Sample ID: AC01533
Page 12 of 14
SPIKE ADDED
ng/L
120 120 240 24.0 24.0 24.0 120 120
SAMPLE CONCENTRATION
ng/L
10.0 ND 201 ND 32.0 ND ND 8.6
MS
MS
CONCENTRATION
%
ng/L
REC
98.3
74
107
89
409
87
23.9
100
55.5
96
9.75
41
85.6
71
122
95
QC LIMITS (% REC)
55 - 99 24 - 160 34 - 131 61 - 107 48 - 112 17 - 112 31 - 137 41 - 132
22090004$STA
PARAMETER
Acetaminophen Atenolol Caffeine Carbamazepine Cotinine Diphenhydramine Metoprolol Paraxanthine
Comments:
U.S. ENVIRONMENTAL PROTECTION AGENCY, REGION 1 LABORATORY SERVICES BRANCH MS4 Methuen, MA
Laboratory Fortified Blank (LFB) Results
LFB AMOUNT SPIKED ng/L
120 120 240 24 24 24 120 120
LFB RESULT
ng/L
84.1 126 186 19.1 19.3 21.2 112 111
LFB RECOVERY
%
70 105 78 80 80 88 93 93
Page 13 of 14
QC LIMITS
% 45 - 106 33 - 129 41 - 120 63 - 108 55 - 106 15 - 113 41 - 122 52 - 115
Samples in Batch: AC01526, AC01527, AC01528, AC01529, AC01530, AC01531, AC01532, AC01533, AC01534
22090004$STA
Page 14 of 14
City of Methuen, MA (MA041210) EPA MS4 Compliance Sampling Inspection
Attachment B Laboratory Report for
E. coli Analysis Author: LSASD-LSB
Pages: 4
Page 18 of 19
Sampling Inspection Date: 09/06/2022
October 03, 2022
Michelle Coombs - LFSB US EPA New England R1
United States Environmental Protection Agency Laboratory Services and Applied Science Division
11 Technology Drive North Chelmsford, MA 01863-2431
Microbiology Laboratory Report
Project Number: 22090007 Project: MS4 Methuen, MA Analysis: E. Coli Defined Substrate Analyst: Jack Paar
Date Samples Received by the Laboratory: 09/06/2022
Analytical Procedure: All samples were received and logged in by the laboratory according to the USEPA New England Laboratory SOP for Sample Log-in.
Sample preparation and analysis was done following the EPA Region I SOP, TC/ECColilert4.
Sample preparation and analysis was done following the EPA Region I method: Total Coliform/E. coli by Defined Substrate.
Data were reviewed in accordance with the internal verification procedures described in the EPA New England LSASD Biology QA Plan.
Results relate only to the items tested or to the samples as received by the Laboratory. This analytical report shall not be reproduced except in full, without written approval of the laboratory.
If you have any questions please call me at 617-918-8604. Sincerely,
Jack A. Paar, III Biology Laboratory Manager
Page 1 of 4
22090007ECMM
Water Microbiology Laboratory Data Qualifier Codes
J = Estimate H = Exceeds holding time I = Exceeds incubation time At = Atypical overgrowth S = Lost sample V = Insufficient sample volume TNTC = Too numerous to count MB = Media blank +++ = Positive control - - - = Negative control SP = Spiked Sample L = Estimated, result below reporting limit (RL) ND = Not Detected, result less than RL D = Lab Duplicate P = Plate counts outside preferred range FDUP = Field Duplicate collect time not specified on Chain of Custody. Field duplicate LIMS default time = 12:00AM
Page 2 of 4
22090007ECMM
Matrix: Stormwater
UNITED STATES ENVIRONMENTAL PROTECTION AGENCY REGION 1 - NEW ENGLAND
LABORATORY SERVICES BRANCH MS4 Methuen, MA
E. Coli Defined Substrate
Sample Number
Lab ID Date of Collection
CVS CURTIS 47BICK 96EAST BROOKOUT BROOK CEDAR DEARBORN
AC01549 AC01550 AC01551 AC01552 AC01553 AC01554 AC01555 AC01556
09/06/22 09/06/22 09/06/22 09/06/22 09/06/22 09/06/22 09/06/22 09/06/22
10:00 am 10:14 am 10:31 am 10:46 am 11:11 am 11:13 am 11:43 am 11:58 am
Number of Samples: 8
Date of Analysis
09/06/22 09/06/22 09/06/22 09/06/22 09/06/22 09/06/22 09/06/22 09/06/22
2:30 pm 2:30 pm 2:30 pm 2:30 pm 2:30 pm 2:30 pm 2:30 pm 2:30 pm
Compound
E. Coli Defined Substrate E. Coli Defined Substrate E. Coli Defined Substrate E. Coli Defined Substrate E. Coli Defined Substrate E. Coli Defined Substrate E. Coli Defined Substrate E. Coli Defined Substrate
Concentration MPN/100 mL
3080 2908 9678 3080 689 2190 3683 3683
RL MPN/100 mL
4 4 4 4 4 4 4 4
Qualifier
Page 3 of 4
22090007ECMM
Page 4 of 4
City of Methuen, MA (MA041210) EPA MS4 Compliance Sampling Inspection
Attachment C Laboratory Report for Enterococcus Analysis Author: LSASD-LSB
Pages: 4
Page 19 of 19
Sampling Inspection Date: 09/06/2022
October 03, 2022
Michelle Coombs - LFSB US EPA New England R1
United States Environmental Protection Agency Laboratory Services and Applied Science Division
11 Technology Drive North Chelmsford, MA 01863-2431
Microbiology Laboratory Report
Project Number: 22090007 Project: MS4 Methuen, MA Analysis: Enterococcus in Water Analyst: Jack Paar
Date Samples Received by the Laboratory: 09/06/2022
Analytical Procedure: All samples were received and logged in by the laboratory according to the USEPA New England Laboratory SOP for Sample Log-in.
Sample preparation and analysis was done following the EPA Region I SOP, ECA Enterolert3.
Sample preparation and analysis was done following the EPA Region I method: Enterococcus by Defined Substrate
Data were reviewed in accordance with the internal verification procedures described in the EPA New England LSASD Biology QA Plan.
Results relate only to the items tested or to the samples as received by the Laboratory. This analytical report shall not be reproduced except in full, without written approval of the laboratory.
If you have any questions please call me at 617-918-8604. Sincerely,
Jack A. Paar, III Biology Laboratory Manager
Page 1 of 4
22090007ENTERO
Water Microbiology Laboratory Data Qualifier Codes
J = Estimate H = Exceeds holding time I = Exceeds incubation time At = Atypical overgrowth S = Lost sample V = Insufficient sample volume TNTC = Too numerous to count MB = Media blank +++ = Positive control - - - = Negative control SP = Spiked Sample L = Estimated, result below reporting limit (RL) ND = Not Detected, result less than RL D = Lab Duplicate P = Plate counts outside preferred range FDUP = Field Duplicate collect time not specified on Chain of Custody. Field duplicate LIMS default time = 12:00AM
Page 2 of 4
22090007ENTERO
Matrix: Stormwater
UNITED STATES ENVIRONMENTAL PROTECTION AGENCY REGION 1 - NEW ENGLAND
LABORATORY SERVICES BRANCH MS4 Methuen, MA
Enterococcus in Water
Sample Number
Lab ID Date of Collection
CVS CURTIS 47BICK 96EAST BROOKOUT BROOK CEDAR DEARBORN
AC01549 AC01550 AC01551 AC01552 AC01553 AC01554 AC01555 AC01556
09/06/22 09/06/22 09/06/22 09/06/22 09/06/22 09/06/22 09/06/22 09/06/22
10:00 am 10:14 am 10:31 am 10:46 am 11:11 am 11:13 am 11:43 am 11:58 am
Number of Samples: 8
Date of Analysis
09/06/22 09/06/22 09/06/22 09/06/22 09/06/22 09/06/22 09/06/22 09/06/22
2:30 pm 2:30 pm 2:30 pm 2:30 pm 2:30 pm 2:30 pm 2:30 pm 2:30 pm
Compound
Enterococcus in Water Enterococcus in Water Enterococcus in Water Enterococcus in Water Enterococcus in Water Enterococcus in Water Enterococcus in Water Enterococcus in Water
Concentration MPN/100mL
8164 19863 12997 5172 3654 1317 12033 8664
RL MPN/100mL
10 10 10 10 10 10 10 10
Qualifier
Page 3 of 4
22090007ENTERO
Page 4 of 4