Document a44nDO7k9ab616BVagBvk1VpX

Summary Report 104-Week DietaryChronicToxicityand CarcinogenicitSytudy withPerfluorooctanSeulfonicAcid Potassium Salt(PFOS: T-6295) inRats PREPARED FOR: 3M COVANCE STUDY NUMMER: 6329-183 SUMMARY REPORT -WEEK 53 104Week DietarTyoxiciatnydCarcinogeniSctiutdyywithPerfluorooctane SulfonicAcid PotassiumSalt(PFOS; T-6295)inRats Timeline Covance 6329-183: In-lifsetartdate:April20, 1998 Week 53 completed:April25, 1999 Introduction The purposeofthisstudyisto assessthechronictoxicitaynd carcinogeniciotfythetest materialT,-6295 (PerfluorooctanseulfoniaccidpotassiumsaltP,FOS), when administered inthedietto ratsforatleast104 weeks. Thissummary reportincludesinformation throughWeek 53 and containscurrentfindingsi;tisnot ,however,thefinarleportforthe study.Covance LaboratorieIsnc.reservestherightofreviewofthesedatabeforeissuing a finalreport. Methods Male and femaleCrl:CD"(SD)IGS BR ratswere assignedto groupsaccordingto the followingdesign: Group I (Control) 2 (LOW)B 3 (Mid)b 4 (Mid-Hlgh)b 5 (High)b,d 6 (Mid-ffigbRecovery) Number Male 70 60 60 70 70 40 of Animals Female 70 60 60 70 70 40 DietaryLevels (ppmT-6295)r 0 0.5 2.0 5.0 20.0 20.0 a The controlanimalsreceivedthebasaldietonly. b Five animals/sexin Groups I through 5 were -,acrificdeudring Weeks 4 and 14 forhepatocellulaprroliferatiornatemeasurements, biochemicalanalyses(paimitoyl-CoAoxidation)m,itochondrial activitaynalyses,and histopathology(Week 14 only). c T-6295 isperfluorooctanseulfonicacidpotassium (PFOS); dose levels are expressedas ppm of T-6295 d Ten animals/sexin Groups I and 5 were designateda,,i,nterim sacrificaenimals. These animals were sacrificeadfteratleast 52 weeks of treatment.. Covance 6329-183 3M T-6295 Observadon ofAnimals Food was providedad libitume,xceptwhen animalswere fasted.Water was provided ad libitum.The animalswere observedtwicedaily(a.m.and p.m.)formortalitaynd moribundityf;indingwsere recordedas theywere observed.At leastonce priorto treatmentand weekly thereaftere,ach anirnawlas removed from itscage and examined; abnormalfindingosr an indicatioonf normalwas recorded.Body weightdatawere recordedweekly foreach aniinaflorWeeks I through17 and once every4 weeks thereafterF.ood consumptiondatawere collectefdoreach animalforWeeks I through16 and once every4 weeks thereafter. ClinicalPathology. Blood and urinesampleswere collectefdorhematology,clinical chemistry,urinalysiasn,d urinechen3istrfyrom 10 animaLVsex/groupinGroups I through5 duringWeeks 4, 14,27,and 53. Animalswere fastedovernighta,nd urinewas collectecdhilleodvernight(approximatel1y6 hours)beforebloodsampling.Blood was collectefdrom a jugularvein.The anticoagulanwtas potassiumEDTA forhematology tests.The samplesforclinicaclhemistryand serum sampleswere collectewdithout anticoagulantW.hen possiblea,blood filmwas made forpossiblefutureexamination from animalssacrificeadtunscheduledintervalsA. bloodfilmwas alsomade and heldfor possiblefutureexaminationfrom animalssacrificedduringWeek 53. Serum PFOS Analyses. DuringWeeks 4 and 14,bloodsampleswere collectefdor serum analysesfrom fiveanimalstsex/groiunpGroups I through5. During Week 53, blood sampleswere collectefdrom fiveanimals/sex/grouipnGroups 1 and 5. Animals were fastedovernight.Blood (approximatel2ymL) was collectefdrom ajugularvein, allowedto clotatroom temperaturea,nd centrifugedS.ampleswere collectewdithout anticoagulantS.erum was harvestedand storedina freezers,ettomaintain-60 to-800C, untilsenttotheSponsor foranalysesof PFOS levels. Temiination. During Weeks 4 and 14,fiveanirnals/sex/group/inteirnvGarloups I through5 were fastedovernightb,ledforserum chemistrya,nesthetizewdithcarbon dioxide,weighed,and exsanguinated.The abdominalcavityof each animalwas opened, theliverwas removed and weighed. Liver samples were collectedforpahnitoyl-CoA, cell proliferatioann,d PFOS concentratioannalysesand forinitochondriaacltivit(yWeek 4 sacrificoenly)and histopatholog(yWeek 14 sacrificoenly).Animalssacrificedduring Week 4 were discardedafterEvercollectionD.uringWeek 53, 10 animals/sex/groufprom 2 Covance 6329-183 3M T-6295 Groups I and 5 were fastedovernight,bledforserum samples (fiveanimaL-/sex/group), anesthetizedwithcarbon dioxide,weighed, exsanguinateda,nd necropsied.The liverwas weighed and liversamples were collectedand frozenforanalysis.Animals sacrificed duringWeeks 14 and 53 had macroscopic observationsrecorded,selectedorgans weighed, and selectedtissuescollectedand preserved.Adrenals,brain,eyes,kidneys, liverm,esentericlymph node,pancreas,spleen,testesa,nd ovarieswere processed and examined microscopicallfyrom animalssacrificedduringWeek 14 and on alltissuesfrom the animalsinGroups 1 and 5 thatwere necropsiedduringWeek 53. Animals thatdiedor were sacrificeadtunscheduled intervalwsere alsonecropsiedand microscopic examinationswere done on selectedtissuescollectedb,ut organ weightswere not recorded.A blood filmwas takenforeach animalsacrificeadtunscheduledintervalasnd atthe Week 53 interimsacrifice. ProliferationCellNuclear Antigen (PCNA) Evaluation. During theWeek 4 and 14 tissuecollectionfsrom animalsinGroups I through5 (fiveanimals/sex/group), representativseamples oftheleftlaterallobeand any macroscopiclesionsofthe liverwere collectedand preservedinzincformalin.Liversampleswere fixedand embedded, and blockswere sentto PathologyAssociatesInternationa(lPAI);PAI willevaluatethe samples(lefltaterallobeonly)forPCNA. In additionl,iversectionswillbe stainedwith hematoxyhn and eosinand examined microscopically. Palniitoyi-CoA (PCOA) Oiddase Analyses. During theWeek 4 and 14 tissuecollections from animalsinGroups 1 through 5 (fiveanimaL-/sex/groupa),sample (approximately 500 mg) ofthe rightlaterallobeoftheliverwas collectedand flash-frozeinnliquid nitrogen.The samples were storedina freezersettomaintain-60 to -800C, until analyzedby Covance forPCOA oxidaseactivity. PFOS Analysis. During theWeek 4 and 14 tissuecollectionfsoranimalsin Groups I through5 (fiveanimals/sex/groupa)nd Week 53 foranimalsinGroups I and 5 (fiveanimaLVsex/group)a, portionoftheremainingliverwas flash-frozeinnliquid nitrogenand storedina freezersettomaintain-60 to-80'C, untilsentto theSponsor. The Sponsor willanalyzetheliversamples forPFOS and metabolites. 3 Covance 6329-183 3M T-6295 MitochondrialAnalyses.DuringtheWeek 4 tissuceollectiofnosranimalsinGroups I through5,hversamples(approximatel1y.5g) were conectedand analyzedby the Sponsor formitochondriaalctivity. Bromodeoxyuxidine InununohistocheinistryS.even daysbeforetheWeek 53 interim sacrificoes,moticpumps (ALZET@ Model 2MLI) were surgicauyimplantedfrom fiveaniinaLs/sex/grofurpom Groups I and 5. The osmoticpumps were preloadedwith approximatel2y mL ofbromodeoxyuridine(BrdU) ata concentratioonf20 mg/mL. The animalswere anesthetizebdy administrationf acepromazine-ketamine-xylazinaen,d one pump/animalwas asepticalilnysertesdubcutaneousl(ydorsalsurface)T.he incision was closedwithwound chps,and theanimalswere monitoredduringclinicaolbservations untilthetimeof sacrificteo ensurethatthereareno chnicalsignsofinfectionO.ne Group 5 male implantedwith osmoticpump was sacrificebdeforetheWeek 53 sacrifice. At theWeek 53 sacrificBer,dU immunohistochemistrwyas performedby PAI on the liverasnd duodenums from thefiveanimaLs/sex/groufprom Groups I and 5 thatreceived BrdU. In additions,ectionsoftheliverasnd duodenums from theseanimalswere stained withhematoxya and eosinand examinedmicroscopicallyR.esultswillbe providedfor inclusioinnthefinalreport. Results ClinicalObservationsand Survival.Adjustedsurvivadlataaresummarized inTable 1 ClinicaolbservationdataaresummarizedinTable2. Survivalafter53 weeks of treatment was 96.0%, 96.0%, 100%, 94.0%, 96.0%, and 100% formales inGroups 1,2,3,4,5, and 6,respectivelayn,d 100%, 94.0%, 94.0%, 96.0%, 98.0%, and 98.0% forfemalesin Groups 1,2,3,,4, 5,and 6,respectively. There were no apparenttestmaterial-relatceldinicaolbservationnsotedthroughWeek 53. Body Weights. Body weightand body weightchange dataaresummarized inTables3 and 4. Males given20.0ppm had significantlloywermean body weightscompared to thoseof animalsgiventhecontrolmaterialduringWeeks 9 through53,and femalesgiven 20.0 ppm had significantlloywerbody weightscompared to thoseof animalsgiventhe controlmaterialduringWeeks 3 through53. Mean body weightsformalesand females were similairnalltheothertreatedgroupscompared to thoseof animalsgiventhecontrol 4 Covance 6329-183 3M T-6295 material.Males and females given 20.0 ppm gained lessweight during the overallperiod from Weeks I through 53; the differenceswere statisticalsliygnificanftor the females. Animals at lower dose levelsalso had occasionalstatisticalsliygnificandtecreasesin body weight gain;however, theseoccurrenceswere inconsistentover time between sexes and were not clearlydose related. Food Consumption. Food consumption data are summarized inTable 5. Although not always statisticalsliygnificantm,ales given 20.0 ppm tended to consume lessfood during Weeks I through 24. Food consumption was similarformales given 20.0 ppm compared to those of animalsgiven thecontrolmaterialduringWeeks 28 through 52. Statistically significantlyower food consumption was noted forfemales given 20.0 ppm during Weeks 2 through 44. Food consumption for males and females were similarin allthe othertreatedgroups compared to those of animals given the controlmaterial. Test Material Consumption. Test materialconsumption dataare sun-unarizedin Table 6. Animals were fed dietsintendedto provide0, 0.5,2.0,5.0,20.0 opm. The amount of testmaterialconsumed by animalson a mg/kg of body weight/day basiswas as follows: Target Dose LeveLs (ppm) 0.5 2.0 5.0 20.0 20.0 Achieved Dose Levels -Range (mg/kg body weight/day) Males Females 0.017-0.057 0.071-0.226 0.174-0.570 0.714-2.205 0.732-2.336 0.022-0.052 0.095-0.213 0.237-0.559 1.069-2.149 1.047-2.160 Clinicaland Anatomic Pathology. Hematology, clinicaclhemistry,urinalysisa,nd urine chemistrydata are summarized inTables 7 through 22. Terminal body weights,absolute organ weights,organ-to-bodyweight percentages,and organ-to-brainweight ratiosare summarized inTables 23 and 1.4.Incidencesof macroscopic and microscopic observationsare summarized inTables 25 through 29. The Pathology Report containsa discussionof the data. 5 1. f Covance 6329-183 3M T-6295 Dose Analyses. Results of the homogeneity, stabilitya,nd dose preparation analyses are inTables 30 through 32. Severalhomogeneity analyseswere conducted on each of severalmixes in an attempt to resolveissuesrelatedto the inherentvariabilitaynd lackof sufficienstensitivitiyn the analyticamlethod.formeasuring (PFOS-,T-6295). Mean valuesof thehomogeneity analysesforthe n-iidxone on March 18, 1998, ranged from 119-135%, and 107-118% of the theoreticacloncentrationsforthedietscontaining0.5 and 20 ppm (PFOS; T-6295), respectively.Mean valuesof the homogeneity analysesfor the niixdone on April 16, 1998, ranged from 96.2-122%, 98.5-107%, 97.6-101%, and 90.0-101% of the theoreticacloncentrationsforthedietcontaining0.5,2, 5, and 20 ppm (PFOS; T-6295), respectively.Mean valuesof the homogeneity analysesfor the mix done on April22, 1998, ranged from 93.5-103% of thetheoreticacloncentrationsforthe diet containing20 ppm (PFOS; T-6295). Mean valuesof thehomogeneity analysesforthe niixdone on June 18, 1998, ranged from 91.0-175%, 111-130%, 99.6-102%, and 90.5-97.0% of the theoreticacloncentrationsforthe dietcontaining0.5,2, 5, and 20 ppm (PFOS, T-6295). These resultsindicatethatthe mixing procedure produced a homogeneous distributioonf the testmaterialinthe dose preparations;although variability generallyappeared slightlgyreateratthe lower dietaryconcentrations. Stabilitaynalyseswere conducted prestudy in an attempt to resolveissuesrelatedto the inherentvariabilitaynd lackof sufficienstensitivitayt levelsinthe analyticamlethod for measuring (PFOS; T-6295). Resultsof stabilitaynalysesfortheniixconducted on March 18, 1998, of samples storedfor28 days atroom temperatureindicatedthatthe mean concentrationswere 78.8 %, 76.7%, 79.8% and 88.1% of the initiacloncentrations of 0.5,1,2, and 20 ppm, respectively.Resultsof stabilitaynalysesfor the mix conducted on April 16, 1998, of samples storedfor 33 days atroom temperatureindicatedthatthe mean concentrationswere Ill-.3%,119.6%, 113.9% and 126.7% of the initial concentrationsof 0.5,1,2, and 20 ppm, respectively. The mean concentratioonfsthedosepreparatioannalysefsorallevelsrangedfrom 44.4%-276% of the theoretical concentrations (including the reassay and retention sample analyses). Inherent variability and lack of sufficient sensitivity at low levels in the analyticamlethod formeasuring (PFOS; T-6295) resultedin homogeneity, stabilitayn,d routineanalysisdata thatwere in many cases outsideof the standardlimitsof +/- 15%. 6 Covance 6329-183 3M T-6295 These resultwsillbe reevaluateidnconjunctionwiththeanalyticarlesultfsrom theblood and liverleveldeterminationwshen theyareprovidedby theSponsor. 7 PATHOLOGY REPORT Week 14 and 53 InterimSacrifices Covance 6329-183 3M T-6295 SUMMARY The purposeof thisstudyisto assessthechronictoxicitaynd carcinogeniciotfythetest materialP,erfluorooctanSeulfuriAccid PotassiumSalt(PFOS) when administereidnthe diettoratsforatleast104 weeks. The testmaterialisbeingadministereadtdose levelsof 0.5,2.0,5.0,and 20.0ppm. Thisinterimreportdiscussefsindingtshroughthefirst 53 weeks ofstudy. Dietaryadministrationf PFOS forapproximately53 weeks was associatewdithmildly higherureanitrogenformales and femalesfed5.0or 20.0ppm; mildlylower glucosefor males and femalesfed20.0 ppm; mildlyto moderatelylower cholesteroflormales and femalesfed20.0 ppm; and mildlyhigheralanineaminotransferasfeormalesfed20.0 ppm. None oftheseeffectwsere consideredadverse.There was no effecton hepatic pahnitoyl-CoAoxidaseactivity. Terminalbody weightsinmalesand femalesatWeek 14 were comparablebetween control and treatedgroups.At theWeek 14 interimsacrificaeb,soluteand relativleiverweights were significantilnycreasedinthemales given20.0ppm. In femalesgiven20.0 ppm, only theliver-to-bodwyeightpercentagewas significantilnycreased.Test material-related histomorphologichangeswere limitedtotheEverinthemalesgiven5.0or 20.0 ppm and inthefemalesgiven20.0 ppm. The changesconsistedofhypertrophyof hepatocytesin centrilobulareasinmales and females,and midzonalto centrilobulhaerpatocytic vacuolation.The incidenceand severitoyf thechangestendedto be greaterinthemales. At theWeek 53 interimsacrifictee,rminalbody weightswere significantdleycreasedinthe femalesgiven20 ppm. Inthemales,absoluteand relativleiverweightswere increasedin thegroup receiving20 ppm. Inadditiona,bsoluteand relativsepleenweightswere decreasedinmalesgiven20 ppm. There were no clearorconsistengtrossobservationsat theWeek 53 sacrifictehatcouldbe attributetdo theadministratiofnthetestmaterial.At theWeek 53 sacrificcee,ntrilobulhaerpatocytihcypertrophyand vacuolationwas 8 Covance 6329-183 3M T-6295 increasedinincidenceand severityinthe males given 20 ppm. In the females given 20 ppm, generally,only centrilobuluhypertrophywas seen,and the change was less severethan thatnoted in the males. In addition,minimal to slightcentrilobulahrepatocytic pigment was found in thefemalesgiven 20 ppm. Ilere were no otherhistomorphologic changes thatcould be associatedwith the administrationo-f the testmaterial.Findingsin liversinseveralunscheduled deaths given 20 ppm resembled those seen inanimals sacrificedatWeek 53. METHODS Six groups of Crl:CD (SD)IGS BR ratswere studiedusing the following study design. Group I (Control) 2 (Low) 3 (Mid) 4 (Mid-High) 5 (High 6 (High Recovery) Number Male 70 60 60 60 70 40 of Animals Female 70 60 60 60 70 40 DietaryLevels (ppm T-6295) 0 0.5 2.0 5.0 20.0 20.0 During Weeks 4, 14, 27, and 53, blood and urinewere collectedfor hematology, clinical chemistry,urinalysisa,nd urinechemistrytestsfrom 10 animawsex inGroups 1 through 5. Five animaLVsex inGroups I through 5 were sacrificeduring Week 4; liverswere collectedand weighed. A portion.ofthe liverwas shipped to the Sponsor forPFOS analysisand mitochondrialactivitya,portionwas shipped to Pathology Associates Internationaflor hepatocerularproliferatiornatemeasurements by proliferatiocnell nuclearantigen(PCNA), and a thirdportionwas used fordeterminationof pahnitoyl-CoA oxidase activity.During Weeks 14 and 53, necropsieswere performed on fiveand 10 animaLs/sex.respectivelyi,nGroups I through 5. At necropsy,macroscopic observationswere recorded,organ weights were obtained,and tissueswere placed in fixativeas specifiedby the protocol. In addition,liversamples were collectedfor PFOS analysis(Weeks 14 and 53),hepatoceuularprolfferatiornatemeasurement [by PCNA at Week 14 and by bromodeoxyuridine (BrdU) immunohistochemistry atWeek 53] and palmitoyl-CoA oxidasedetermination(Week 14). A sectionof duodenum was also 9 Covance 6329-183 3M T-6295 collectedatWeek 53 forBrdU immunohistochemistry.At unschedulednecropsiesfor animalsthatdiedor were sacrificebdecauseof poor health,macroscopic observations were recorded,and tissueswere placedinfixativeb,ut organ weightswere not obtained. Microscopicexaminationswere done on selectedtissues(adrenalsb,rain,eyes,kidney, liverm,esentericlymph node, pancreas,spleen,testes,and ovaries)from the animals necropsiedduringWeek 14 and on alltissuesfrom the anijrnailnsGroups 1,and 5 that were necropsiedduringWeek 53. In additionm,icroscopicexaminationswere done on the tissuesfrom animalsthatdiedor were sacrificeddue topoor health. StatisticalsliygnificandtifferencecsitedintheResultsand Discussionsectionarebased on comparisons between thecontroland treatedgroups. RESULTS AND DISCUSSION ClinicalPathology Weeks 4, 14,27, and 53. There were relativelfyew statisticaslilgynificanotr otherwise notabledifferencefsorclinicaplathologyresultsbetween the controland treatedgroups. Those differencetshatwere themost consistenotver timewere consideredto be associatedwithadministratioonfthe testmaterial.These includedmildlyhigherurea nitrogenformales and femalesfed 5.0or 20.0 ppm-,mildlylower glucoseformales and femalesfed 20.0ppm; mildlytomoderatelylower cholesteroflormales and femalesfed 20.0 ppm; and mildlyhigheralanineaminotransferasfeormales fed20.0 ppm. There were no correlativmeicroscopicrenalfindingsforthe minor change inurea nitrogen.The findingsforalanine-aminotransferaasned,possiblyforglucoseand cholesterolw,ere likelyassociatedwith thehistopathologicfailndingsof hepatocerular hypertrophyand vacuolation.None oftheclinicaplathologyeffectswere considered adverse. Of uncertainrelationshitpothetestmaterialwere statisticahlilgyherureanitrogenfor males fed 2.0ppm and statisticalolwyer glucoseforfemalesfed 2.0 or 5.0 ppm at Week 53. These findingswere not asconsistenotver timeas thoseconsideredto be effectsof the testmateriala,nd themagnitudes of thesedifferencewsere very smar. 10 Covance 6329-183 3M T-6295 AD otherstatisticalsliygnificandtifferencefsorclinicaplathologyresultsbetween the controland treatedgroups were consideredincidentalM.ost ofthesedfferencesdidnot affectanimalsfedthe highestdose level,and none of thesedfferenceswere consistent over time. Anatomical Pathology Week 14,Terndnal Body Weight and Organ Weights. Terminalbody weightswere comparable between controland treatedgroups. Absoluteand relativleiverweightswere significantilnycreasedinthemales given20.0 ppm. Infemalesgiven20.0 ppm, onlythe Ever-to-bodyweight percentagewas significantilnycreased.The absolutespleenweight was significantdleycreasedinthefemalesgiven20.0 ppm, as was the absolutelung weight infemalesgiven 2.0,5.0,or 20.0ppm. Spurious,significainntcreasesinleft thyroid/parathyroid-to-bowdeyight percentageswere seen infemalesgiven5.0 or 20.0 ppm. Macroscopic Observations. There were no macroscopic observationsthatcould be attributetdo theadministratioonf thetestmaterial. Microscopic Observations. Test material-relatheidstomorphologicchanges were limited to theliverinthemales given5.0 or 20.0ppm and inthefemalesgiven 20.0ppm. The changes consistedof hypertrophyofhepatocytesincentrilobulaarreasand midzonal to centrilobulahrepatocyticvacuolation.The incidenceand severityof thechanges tended to be greaterinthemales. There were no potentialltyestmaterial-relatleedsionsintheremainingtissuesexamined. Week 53 Interim Sacrifice,Body and Organ Weights. In thefemales,terminalbody weights were significantdleycreasedinthegroup given20 ppm. Terminal body weights in themales were comparable between controland treatedgroups. In themales,absoluteand relativleiverweightswere increasedinthegroup receiving 20 ppm. In addition,absoluteand relativsepleenweightswere decreasedinmales given 20 ppm. Significantdleycreasedleftthyroid/parathyrowiedightswere consideredto be spuriousdue to the absence of a contralateraclffect. Covance 6329-183 3M T-6295 In view of the significandtecreasedin body weight infemales given 20ppm, significant increasesin organ-to-body weight percentagesfor brain,kidney,liver,and spleenmay be of no toxicologicailmportance. Decreased absoluteweights in the leftadrenalgland and bilateraaldrenal-to-braiwneight ratiosmay alsorepresentchanges secondary to the body weight loss. Macroscopic Observations. There were no clearor consistentgross observationsat the Week 53 sacrifictehatcould be attributedto the administrationof the testmaterial. .Microscopic Observations. Centrilobularhepatocytichypertrophy and vacuolationwas increasedinincidenceand severityin the males given 20 ppm. In the females given 20 ppm, generally,only centrilobulahrypertrophy was seen,and the change was less severe than thatnoted in the males. In addition,minimal to slightCentrilobular hepatocyticpigment was found inthe females given 20 ppm. There were no other histomorphologicchanges thatcould be associatedwith the administrationof the test material. Unscheduled Deaths Through Week 53 Large, mottled,or dfffuselydark Evers were noted in 2/3 males and 1/1 females given 20ppm. There were no othergross observationsthatcould be attributedto the administrationof the testmaterial.Microscopicallys,everalanimalsgiven 20 ppm exhibitedliverchanges thatwere similaror relatedto thoseseen at the Week 53 Interim Sacrifice. Robert L. Hall,DVM, PhD Diplomate, ACVP (ClinicaPlathology) Richard D. Alsaker,DVM, MS Diplomate, ACVP Diplomate, ABT Date Date 12 TABLE 1 Col summary of )Ldjusted survival Data 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ---------------------------------------------------------------------------------------------------------------------- GROUP AND DOSE LEVEL (PPM) ---------- 0 2 .500 3 2 4 5 5 20 6 20 WEEK: START 1 2 3 4 5 6 7 a 9 10 -------------------------------------------------------------------------------------------------------- 70/70 70/70 70/70 70/70 MALE 65/65 65/65 65/65 65/65 65/65 65/65 65/65 60/60 60/60 60/60 60/60 55/55 55/55 55/55 55/55 55/55 55/55 SS/55 60/60 60/60 60/60 60/60 55/55 55/55 55/55 55/55 55/55 55/55 55/55 60/60 60/60 60/60 60/60 55/55 55/55 55/55 55/55 55/55 55/55 55/55 70/70 70/70 70/70 70/70 65/6S 65/65 65/6S 65/65 65/65 65/65 65/65 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 TABLE 1 c Summary of Adjusted Survival Data 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS -------------------------------------------------------------------------------------------------------------------- GROUP AND DOSE LEVEL (PPM) ---------- 0 2 .500 3 2 4 5 5 20 6 20 WEEK. 12 13 14 15 16 17 18 19 20 21 22 ------------------------------------------------------------------------------------------------------ 65/65 65/65 60/60 60/60 KALE 60/60 60/60 60/60 60/60 60/60 60/60 60/60 55/55 55/55 50/50 50/50 50/50 50/50 50/50 49/50 49/50 49/50 49/50 55/55 55/55 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 55/55 55/55 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 65/65 65/65 60/60 60/60 60/60 60/60 60/60 60/60 60/60 601/60 60/60 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/4 TABLE 1 c Summary of Adjusted survival Data 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANS SULFONIC ACID POTASSIUM SALT IN RATS --------------------------------------------------------------------------------------------------------------------- GROUP AND DOSE LEVEL (PPM) ---------- 0 2 .500 3 2 4 5 5 20 6 20 WEEK: 24 25 26 27 29 29 30 31 32 33 34 ------------------------------------------------------------------------------------------------------- 59/60 59/60 59/60 59160 KALE 59/60 59/60 59/60 59/60 59/60 59/60 59/60 49/50 49/50 49/50 49/50 49/50 49/50 49/50 49/50 49/.50 49/50 49/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/SO 50/50 50/50 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 TABLE 1 Co Summary of Adjusted survival Data p 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS ---------------------------------------------------------------------------------------------------------------------- GROUP AND DOSE LEVEL (PPM) ---------- 1 0 2 .500 3 2 4 5 5 20 6 20 WEEK: 36 37 38 39 40 41 42 43 44 45 46 -------------------------------------------------------------------------------------------------------- 59/60 59/60 59/60 59/60 MALE 59/60 59/60 59/60 59/60 59/60 59/60 59/60 49/50 49/50 49/50 48/50 48/50 48/50 48/50 48/50 48/50 48/50 48/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/SO 50/50 50/50 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 TABLE 1 c sumary of Adjusted Survival Data 104-WMM DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS --------------------------------------------------------------------------------------------------------------------- GROUP AND DOSE LEVEL (PPM) ---------- 0 2 .500 3 2 4 5 5 20 6 20 WEEK-. 48 49 50 51 52 53 ------------------------------------------------------------------------------------------------------ 58/60 58/60 58/60 58/60 MALE 58/60 48/50 48/50 48/50 48/50 48/50 48/50 48/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 49/50 47/50 58/60 59/60 58/60 58/60 57/60 48/51 40/40 40/40 40/40 40/40 40/40 40/40 TABLE I c summary of Adjusted survival Data p 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ---------------------------------------------------------------------------------------------------------------------- GROUP AND DOSE LEVEL (PPM) ---------- 1 0 2 .500 3 2 4 5 5 20 6 20 WEEK: START 1 2 3 4 5 6 7 8 9 10 -------------------------------------------------------------------------------------------------------- 70/70 70/70 70/70 70/70 FEMALE 65/65 65/65 65/65 65/65 65/65 65/65 65/65 60/60 60/60 60/60 60/60 SS/55 55/55 55/55 55/55 55/55 55/S5 55/55 60/60 60/60 60/60 60/60 55/55 55/55 55/55 55/55 55/55 54/55 54/55 60/60 60/60 60/60 60/60 55/55 55/55 55/55 55/55 55/55 55/55 55/55 70/70 70/70 70/70 70/70 65/65 65/65 65/65 65/6S 65/65 65/65 65/65 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 TABLE 1 Co Summary of Adjusted Survival Data p 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ---------------------------------------------------------------------------------------------------------------------- GROUP AND DOSE LEVEL ippm) ---------- WEEK: 12 13 14 15 16 17 18 19 20 21 22 -------------------------------------------------------------------------------------------------------- 0 2 .500 3 2 4 5 5 20 6 20 6S/65 55/55 54/55 55/55 65/65 40/40 65/65 5S/55 54/55 55/55 65/65 40/40 60/60 50/50 49/50 50/50 60/60 40/40 60/60 50/50 49/50 50/50 60/60 40/40 FEMALE 60/60 50/50 49/50 50/50 60/60 40/40 60/60 50/50 49/50 50/50 60/60 40/40 60/60 SO/50 49/50 SO/50 60/60 40/40 60/60 50/50 49/50 50/50 60/60 40140 60/60 50/50 49/50 50/50 60/60 40/40 60/60 SO/50 49/50 50/50 60/60 40/40 60/60 50/50 49/50 50/SO 60/60 40/40 TABLE 1 Co su=tkary of Adjusted surviv&l Data 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PE"LUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS ---------------------------------------------------------------------------------------------------------------------- GROUP AND DOSE LEVEL (PPM) ---------- WEEK: 24 25 26 27 28 29 30 31 32 33 34 -------------------------------------------------------------------------------------------------------- 0 2 .500 3 2 4 5 s 20 6 20 60/60 50/50 49/50 50/50 60/60 40/40 60/60 50/50 49/50 50/50 60/60 40/40 60/60 50/50 49/50 50/50 60/60 40/40 60/60 50/50 49/50 50/SO 60/60 40/40 FEMALE 60/60 50/50 49/50 50/50 60/60 40/40 60/60 50/50 49/50 50/50 60/60 40/40 60/60 50/50 49/50 50/50 60/60 40/40 60/60 50/50 49/50 49/50 60/60 40/40 60/60 50/50 49/50 49/50 60/60 40/40 60/60 49/50 49/50 49/50 60/60 40/40 60/60 49/50 49/50 49/50 60/60 40/40 TABLE 1 c Summary of Adjusted Survival Data 104-WMM DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTAN]3 SULFONIC ACID POTASSIUM SALT IN RATS ---------------------------------------------------------------------------------------------------------------------- GROUP AND DOSE LEVEL (PPM) ---------- 0 2 .500 3 2 4 5 5 20 6 20 WEEK: 36 37 38 39 40 41 42 43 44 45 46 -------------------------------------------------------------------------------------------------------- 60/60 60/60 60/60 60/60 FEKALE 60/60 60/60 60/60 60/60 60/60 60/60 60/60 49/50 49/50 49/50 49/50 49/50 48/50 48/50 48/50 48/50 48/50 48/50 49/50 49/50 49/50 49/50 49/50 49/50 49/50 49/50 49/50 48/50 47/50 49/50 49/50 49/50 49/50 49/50 49/50 49/50 49/50 49/50 49/50 49/50 60/60 60/60 60/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 40/40 40/40 40/40 40/40 40/40 39/40 39/40 39/40 39/40 39/40 39/40 TABLE 1 Cov Summary of Adjusted Survival Data P) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PMTFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------------- GROUP AND DOSE LEVEL (PPM) ---------- 0 2 .500 3 2 4 5 5 20 6 20 WEEK: 48 49 so 51 52 S3 --------------------------------------------------------------------------------------------------------- 60/60 60/60 60/60 60/60 FEMALE 60/60 50/50 48/50 48/50 47/50 47/50 47/50 47/50 47/50 47/50 47/50 47/50 47/50 47/50 49/50 49/50 49/50 49/50 48/50 48/50 59/60 59/60 59/60 59/60 59/60 49/50 39/40 39/40 39/40 39/40 39/40 39/40 c Table 2 summary of Clinical observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS --------------------------------------------------------------------------------------------------------------------- DAYS 1-371 NUMBER OF ANIMALS AFFECTED CATEGORY SEX: GROUP: DOSE: ------------MALE ------------ 1 2 3 4 5 6 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 --------------------------------------------------------------------------------------------------------------------- *** TOP OF LIST APPEARANCE BENT TAIL CONVULSIONS HEAD TILT 0 1 0 0 0 1 0 0 0 0 0 0 LEFT 0 0 0 0 0 0 RIGHT HUNCHED 0 0 0 0 0 0 0 0 0 1 0 0 LIMITED USE LIMB-HIND-LEFT LIMBS-HIND MALOCCLUSION MISSING 0 0 0 0 1 0 0 0 0 0 2 0 6 6 4 6 8 7 TAIL-DISTAL TEETH PARALYSIS LIMBS-HIN6 SWOLLEN 0 0 0 0 0 0 1 2 0 1 2 1 0 1 0 0 0 0 LIMB-HIND-LEPT MOUTH NOSE PAW-HIND-LEFT TAIL-DISTAL VENTRHL-ABDOMINAL TESTES THIN 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 2 0 0 2 1 0 Coi Table 2 summary of clinical observations p 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ---------------------------------------------------------------------------------------------------------------------- NUMBER OF ANIMALS AFFECTED DAYS 1-371 SEX: ------------KALE ------------ GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ---------------------------------------------------------------------------------------------------------------------- BEHAVIOR ATAXIC <UNSPECIFIED> CIRCLING RIGHT HYPOACTIVE <UNSPECIFIED> MILD SENSITIVE TO TOUCH COLD TO TOUCH LIMBS-HIND ENTIRE BODY DISCHARGE GENITAL RED IN COLOR CLEAR ORAL MILD NASAL RED IN COLOR UNKNOWN RED IN COLOR EXCRETION FEW FECES 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 1 0 0 4 0 1 1 3 1 0 0 0 1 0 0 1 0 1 0 0 1 0 0 2 0 0 0 1 1 0 3 2 0 Cov Table 2 Summary of Clinical Observations p 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------------- NUMBER OF ANIMALS AFFECTED DAYS 1-371 SFX: GROUP: ------------MALE ------------ 1 2 3 4 5 6 CATEGORY DOSE-. 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ----------------------------------------------------------------------------------------------------------------------- EXCRETION NO FECES NONFORMED FECES EYES CLEAR DISCHARGE EYE-LEFT RED DISCHARGE EYE-LEFT EYE-RIGHT EYES SCAB EYE-RIGHT SQUINTED PERI-ORBITAL RESPIRATION AUDIBLE IRREGULAR LABORED SKIN & PELAGE ALOPECIA BROWN HAIR SCROTUM COAT 0 0 0 0 0 0 0 0 0 1 0 2 1 1 0 0 0 0 0 0 3 1 0 0 0 0 1 2 1 2 1 0 0 1 0 0 0 2 0 0 0 0 0 0 0 0 0 0 1 2 1 1 0 0 1 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 Cov Table 2 summary of Clinical observations p 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------------- NUMBER OF ANIMALS AFFECTED DAYS 1-371 CATEGORY SFX: GROUP: DOSE: ------------ KALE ------------ 1 2 3 4 5 6 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ---------------------------------------------------------------------------------------------------------------------- SKIN & PELAGE BLACK SKIN DORSAL PALE ENTIRE BODY RED HAIR COAT HEAD-ENTIRE HEAI)-CRANIAL HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT ROUGH HAIR SORE/SCAB AXILLARY COAT REGION-LEFT DORSAL DORSAL-CFRVICAL-LEFT DORSAL-CERVICAL-RIGHT DORSAL-THORACIC-LEFT DORSAL-THORACIC-RIGHT EAR-RIGHT HEAD-CP-ANIAL HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT LATMLKL-CERVICAL-RIGHT LIMB-FRONT-RIGHT MOUTH PAW-HIND-LEFT 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 2 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 1 0 0 0 0 0 1 c Table 2 Sumary of clinical observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTAME SULFONIC ACID POTASSIUM SALT IN RATS --------------------------------------------------------------------------------------------------------------------- NUMBER OF ANIMALS AFFECTED DAYS 1-371 SF.X: ------------MALE ------------ GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 --------------------------------------------------------------------------------------------------------------------- SKIN & PELAGE SORE/SCAB SHOULDER-RIGHT TAIL VENTRAL-ABDOMINAL VENTRAL-ABWMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT VENTRAL-CERVICAL VENTRAL-CERVICAL-LEFT VENTRAL-CERVICAL-RIGHT YELLOW HAIR COAT MASS(ES) AXILLARY REGION-LEFT WEEK 37 WEEK 38 WEEK 39 WEEK 41 WEEK 44 WEEK 45 WEEK 46 WEEK 48 WEEK 50 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 1 0 0 1 0 1 2 0 0 1 0 0 2 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Co Table 2 Summary of Clinical Observations p 104-WMM DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS ---------------------------------------------------------------------------------------------------------------------- NUMBER OP ANIMALS AFFECTED DAYS 1-371 SEX: ------------MALE ------------ GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ---------------------------------------------------------------------------------------------------------------------- HASS(ES) AXILLARY REGION-LEFT WEEK 51 AXILLARY REGION-RIGHT WEEK 36 WEEK 37 WEEK 45 WEEK 46 WEEK 48 WEEK 53 DORSAL WEEK 11 WEEK 29 WEEK 49 WEEK 51 WEEK 53 DORSAL-CERVICAL-LEFT WEEK 25 PAR-LEFT WEEK 48 EAR-RIGHT WEEK 47 HEAD-MAXILLARY-RIGHT WEEK 43 INGUINAL REGION-LMPT WEEK 43 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 c Table 2 summary of Clinical observations 104-WEEK DIETARY CHRONIC TOXICXTY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTAKE SULPONIC ACID POTASSIUM SALT IN RATS ---------------------------------------------------------------------------------------------------------------------- NUMBER OF ANIMALS AFFECTED DAYS 1-371 SEX: ------------MALE ------------ CATEGORY GROUP: DOSE: 1 2 3 0 0.5 2 4 5 6 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ---------------------------------------------------------------------------------------------------------------------- MASS(ES) INGUINAL REGION-LEFT WEEK 46 WEEK 48 INGUINAL REGION-RIGHT WEEK 28 WEEK 43 WEEK 50 LIMB-FRONT-LEFT WEEK 45 LIMB-FRONT-RIGHT WEEK 47 WEEK 51 LIMB-HIND-RIGHT WEEK 47 SHOULDER-LEFT WEEK 48 SHOULDER-RIGHT WEEK 50 TAIL WEEK 49 VENTRAL-ABDOMINAL WEEK 15 WEEK 27 WEEK 29 WEEK 30 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 Cov Table 2 Summary of clinical observations PA 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------------- DAYS 1-371 NUMBER OF ANIMALS AFFECTED CATEGORY KEYWORD SEX: GROUP: DOSE: -----------M-ALE ------------ 1 2 3 4 s 6 0 0.5 2 5 20 20 QUALIFIER NUMBER: 70 60 60 60 70 40 ----------------------------------------------------------------------------------------------------------------------- KASS(ES) VENTRAL-ABWMINAL WEEK 36 WEEK 39 WEEK 41 WEEK 43 WEEK 45 WEEK 47 WEEK 53 VENTRAL-ABDOMINAL-LEFT WEEK 18 WEEK 36 WEEK 33 WEEK 34 WEEK 40 WEEK 42 WEEK 43 WEEK 44 WEEK 45 WEEK 46 WEEK 49 WEEK 50 WEEK 51 VENTRAL-ABDOMINAL-RIGHT WEEK 25 WEEK 38 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Cov Table 2 summary of clinical observations p 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------------- NUMBER OF ANIMALS AFFECTED DAYS 1-371 SEX: ------------MALE ------------ GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ----------------------------------------------------------------------------------------------------------------------- MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 40 WEEK 44 WEEK 47 WEEK 48 WEEK 50 WEEK 53 VENTRAL-CERVICAL WEEK 37 WEEK 45 WEEK 53 VENTRAL-CERVICAL-LEFT WEEK 3 WEEK 21 WEEK 42 WEEK 47 VENTRAL-CMTVICAL-RIGHT WEEK 47 VENTRAL-THORACIC WEEK 45 VENTRAL-THORACIC-LEFT WEEK 25 WEEK 47 VENTRAL-THORACIC-RIGHT WEEK 10 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Table 2 Sunnary of Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTAKE SULFONIC ACID POTASSIUM SALT IN RATS ---------------------------------------------------------------------------------------------------------------- NUMBER OF ANIMALS AFFECTED DAYS 1-371 SEX: ------------MALE ------------ GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ---------------------------------------------------------------------------------------------------------------- WART LIKE LESIONIS) DORSAL-CERVICAL WEEK 7 EAR-RIGHT WEEK 41 HEAD-KAXILLARY-LEFT WEEK 19 WEEK 31 MOUTH WEEK 46 WEEK 47 NOSE WEEK 46 VENTRAL-ABDOMINAL WEEK 40 VENTRAL-THORACIC-LEFT WEEK 46 SURGERY PERFORMED BY JOHN VAN BERKUH KATHY EWERS PATTIE MURPHY 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 1 0 0 0 2 0 3 0 0 0 2 0 1 0 0 0 1 0 Table 2 Summary of Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------- NUMBER OF ANIMALS AFFECTED DAYS 1-371 SEX: GROUP: ------------MALE ------------ 1 2 3 4 5 6 CATEGORY DOSE: 0 O.S 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ----------------------------------------------------------------------------------------------------------- ANTIBIOTIC ADMINISTERED 0.5 ML END OF LIST s 0 0 0 5 0 Table 2 summary of clinical observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------- NUMBER OF ANIMALS AFFECTED DAYS 1-371 SEX: ----------- FEMALE ----------- CATEGORY GROUP: T)OSE: 1 2 3 0 0.5 2 4 5 6 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ----------------------------------------------------------------------------------------------------------------- *** TOP OF LIST APPEARANCE BENT TAIL CONVULSIONS HEAD TILT LEFT RIGHT HUNCHED LIMITED USE LIMB-HIND-LEFT LIMBS-HIND MALOCCLUSION MISSING TAIL-DISTAL TEETH PARALYSIS LIMBS-HIND SWOLLEN LIMB-HIND-LEFT MOUTH NOSE PAW-HIND-LEFT TAIL-DISTAL VENTRAL-ABDOMINAL TESTES THIN 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 12 9 6 6 5 7 0 0 1 0 0 0 1 2 1 1 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 1 1 Table 2 Surmary of Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------- NUMBER OF ANIMALS AFFECTED DAYS 1-371 CATEGORY SEX: GROUP: DOSE: -----------FEMALE ----------- 1 2 3 4 5 6 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ---------------------------------------------------------------------------------------------------------- BEHAVIOR ATAXIC <UNSPECIFIED> CIRCLING RIGHT HYPOACTIVE <UNSPECIFIED> MILD SENSITIVE TO TOUCH COLD TO TOUCH LIMBS-HIND ENTIRE BODY DISCHARGE GENITAL RED IN COLOR CLEAR ORAL MILD NASAL RED IN COLOR UNKNOWN RED IN COLA)R EXCRETION FEW FECES 0 0 1 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2, 1 0 0 0 0 0 0 0 0 0 0 2 1 1 1 1 0 Table 2 Summary of clinical observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS ---------------------------------------------------------------------------------------------------------------- NUMBER OF ANIMALS AFFECTED DAYS 1-371 SF.X: -----------FEMALE ----------- CATEGORY GROUP: DOSE: 1 2 3 0 0.5 2 4 5 6 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 --------------------------------------------------------------------------------------------------------- EXCRETION NO FECES NONFORMED FECES EYES CLEAR DISCHARGE EYE-LEFT RED DISCHARGE EYE-LEFT EYE-RIGHT EYES SCAB EYE-RIGHT SQUIN D PERI-ORBITAL RESPIRATION AUDIBLE IRREGULAR LABORED SKIN & PELAGE ALOPECIA BROWN HAIR SCROTUM COAT 0 0 1 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 0 2 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 Table 2 summary of Clinical observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ---------------------------------------------------------------------------------------------------------------- NUMBER OF ANIMALS AFFECTED DAYS 1-371 CATEGORY SEX: GROUP: DOSE: -----------FEMALE ----------- 1 2 3 4 5 6 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ------------------------------------------------------------------------------------ SKIN & PELAGE BLACK SKIN DORSAL PALE ENTIRE BODY RED HAIR COAT HEAD-ENTIRE HEAD-CP.ANIAL HEAD-MAXILLARY-LEFT HEAD-MAXILLARY-RIGHT ROUGH HAIR COAT SORE/SCAB AXILLARY REGION-LEFT DORSAL DORSAL-CERVICAL-LEFT DORSAL-CERVICAL-RIGHT DORSAL-THORACIC-LEFT DORSAL-THORACIC-RIGHT EAR-RIGHT HEAD-CRANIAL HEAD-KAXILLARY-LEFT HEAD-MAXILLARY-RIGHT LATEM-CERVICAL-RIGHT LIMB-FRONT-RIGHT MOUTH PAW-HIND-L.EFT 0 1 0 0 0 0 0 1 1 0 1 0 1 0 3 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 2 0 0 0 a 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 1 0 0 0 0 0 Table 2 summary of clinical observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ---------------------------------------------------------------------------------------------------------------- NUMBER OF ANIMALS AFFECTED DAYS 1-371 CATEGORY SEX: GROUP: DOSE: -----------FEMALE ----------- 1 2 3 4 5 6 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 --------------------------------------------------------------------------------------------------------------- SKIN & PELAGE SORE/SCAB SHOULDER-RIGHT TAIL VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT VENTRAL-CERVICAL VENTRAL-CERVICAL-LEFT VENTRAL-CERVICAL-RIGHT YELLOW HAIR COAT MASS(ES) AXILLARY REGION-LEFT WEEK 37 WEEK 38 WEEK 39 WEEK 41 WEEK 44 WEEK 45 WEEK 46 WEEK 48 WEEK 50 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 1 0 2 0 1 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 a 0 0 0 1 0 0 0 0 1 1 0 0 0 1 0 0 0 0 0 3 0 0 0 0 0 0 2 0 0 0 1 Table 2 Summary of Clinical Observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS --------------------------------------------------------------------------------------------------------------- NUMBER OF ANIMALS AFFECTED DAYS 1-371 SEX: -----------FEMALE ----------- GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ------------------------------------------------------------------- HASS(ES) AXILLARY REGION-LEFT WEEK 51 AXILLARY REGION-RIGHT WEEK 36 WEEK 37 WEEK 45 WEEK 46 WEEK 48 WEEK 53 DORSAL WEEK 11 WEEK 29 WEEK 49 WEEK 51 WEEK 53 DORSAL-CERVICAL-LEFT WEEK 25 F.AR-LEFT WEEK 48 EAR-RIGHT WEEK 47 HEAD-MAXILLARY-RIGHT WEEK 43 INGUINAL REGION-LEFT WEEK 43 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 2 0 1 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 Table 2 Summary of clinical observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS -------------------------------------------------------------------------------------------------------------- NUMBER OF ANIMALS AFFECTED DAYS 1-371 CATEGORY SEX: GROUP: DOSE: -----------FEMALE ----------- 1 2 3 0 0.5 2 4 5 6 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 -------------- ------------------------------------------------------------------------------------------------- KASS(ES) INGUINAL REGION-LEFT WEEK 46 WEEK 48 INGUINAL REGION-RIGHT WEEK 28 WEEK 43 WEEK 50 LIMB-FRONT-LEFT WEEK 45 LIMB-FRONT-RIGHT WEEK 47 WEEK 51 LIMB-HIND-RIGHT WEEK 47 SHOULDER-LEFT WEEK 48 SHOULDER-RIGHT WEEK 50 TAIL WEEK 49 VENTRAL-ABDOMINAL WEEK 15 WEEK 27 WEEK 29 WEEK 30 0 0 1 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 Table 2 Summary of Clinical observations 104-WEEK DIETARY PERFLUOROOCTANE CHRONIC TOXICITY AND CARCINOGENICITY STUDY SULPONIC ACID POTASSIUM SALT IN RATS WITH ----- ------------------------------------------------------------------------------------------------------- NUMBER OF ANIMALS AFFECTED DAYS 1-371 SEX: GROUP: DOSE: -----------FEMALE ----------- 1 2 3 4 5 6 0 0.5 2 5 20 20 CATEGORY KEYWORD NUMBER: 70 60 60 60 70 40 ----Q-UA-L-IF-I-E-R------------------------------------------------------------------------ --------------- MASS(ES) VENTRAL-ABDOMINAL WEEK 36 WEEK 39 WEEK 41 WEEK 43 WEEK 45 WEEK 47 WEEK 53 VENTRAL-ABDOMINAL-LEF'r WEEK 18 WEEK 30 WEEK 33 WEEK 34 WEEK 40 WEEK 42 WEEK 43 WEEK 44 WEEK 4S WEEK 46 WEEK 49 WEEK 50 WEEK 51 VENTRAL-ABDOMINAL-RIGHT WEEK 25 WEEK 39 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1 0 0 0 1 1 0 0 0 0 1 0 0 0 0 0 Table 2 summary of Clinical observations 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ---------------------------------------------------------------------------------------------------------------- NUMBER OF ANIMALS AFFECTED DAYS 1-371 SEX - ----------F-EMALE ----------- GROUP: 1 2 3 4 5 6 CATEGORY DOSE: 0 0.5 2 5 20 20 KEYWORD QUALIFIER NUMBER: 70 60 60 60 70 40 ------------------------------------------------------------------------------------------------------------- KASS(ES) VENTRAL-ABDOMINAL-RIGNT WEEK 40 WEEK 44 WEEK 47 WEEK 48 WEEK 50 WEEK 53 VENTRAL-CERVICAL WEEK 37 WEEK 45 WEEK 53 VENTRAL-CERVICAL-LEFT WEEK 3 WEEK 21 WEEK 42 WEEK 47 VENTRAL-CERVICAL-RIGHT WEEK 47 VENTRAL-THORACIC WEEK 45 VENTRAL-THORACIC-LEFT WEEK 25 WEEK 47 VENTRAL-THORACIC-RIGHT WEEK 10 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 1 1 0 0 0 0 0 1 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 0 1 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 Sumary Table 2 of clinical Observations 104-WEEK DIETARY PERFLUOROOCTANE CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------- ------------------------ NUMBER OF ANIMALS AFFECTED DAYS 1-371 CATEGORY KEYWORD SEX: GROUP: DOSE: NUMBER: -----------FEMALE ----------- 1 2 3 4 5 6 0 0.5 2 5 20 20 70 60 60 60 70 40 ----Q-U-AL-I-F-I-ER------------------ --------------------------------------- -------------- WART LIKE LESION(S) DORSAL-CERVICAL WEEK 7 EAR-RIGHT WEEK 41 HF.AD-MAXILLARY-LEFT WEEK 19 WEEK 31 MOUTH WEEK 46 WEEK 47 NOSE WEEK 46 VENTRAL_ABDOMINAL WEEK 40 VENTRAL-THORACIC-LEFT WEEK 46 SURGERY PERFORMED BY JOHN VAN BERKUM KATHY EWERS PATTIE MURPHY 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 a 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 2 0 0 0 0 0 1 0 3 0 0 0 2 0 sumary Table 2 Clinical observations of STUDY WITH -WEEK DIETARY 104 PERFLUOROOCTANE CHRONIC 'roXIcr.TY AND CARCINOGENICITY IUM SALT IN RAT s ------------ SULFONIC ACID POTASS - --------------------------- --------------------------------------------------------------- ------ NUMBER OF ANIMALS AFFECTED DAYS 1-371 CATEGORY KEYWORD QUALIFIER ---------------- ANTIBIOTIC ADMINISTERED Ci.5 ML ... END OF LIST SEX: GROUP: DOSE: -----------FEMALE ----------- 1 2 3 0 0.5 2 4 5 20 20 NUMBER: 70 60 6-0----6-0----70----4-0-------------------------------- -------------------------------- 5 0 0 0 5 0 Covan Table 3 Summary of BoclyWeight Data (9) 104-WL7EK DIETARY PEPYLUOROOCTANE CARCINOGENICITY STUDY CHRONIC TOXICITY AND SIUM SALT IN RATS SULPONIC ACID POTAS *DFAFT* WITH PAGI ------------------------- ---------------------------------------------------------------------------------------- SEX: MALE --------------------------------- 3 4 5 6 GROUP: 1 DOSE: 0 2 0.5 2 5 20 20 PPM ------------ WEEK UNITS: PPM PPM --P-P-M ---------p--p-m---------P-P-M---------------------------------------- -------------------------------------- 1 N 70 MEAN 179 S.D. 18.6 2 N 70 MEAN 237 S.D. 21.8 3 N 70 MEAN 287 S.D. 25.0 4 N 70 MEAN 334 S.D. 27.6 5 N 6S ME" 367 S.D. 31.4 6 N 65 MEAN 399 S.D. 34.3 7 N 65 MEAN 419 S.D. 35.1 60 178 18.7 60 237 20.2 60 290 21.4 60 333 23.9 55 370 27.1 55 402 30.8 55 428 33.3 60 179 19.0 60 237 21.S 60 286 23.0 60 328 25.4 55 367 28.4 55 397 30.3 55 418 31.8 60 179 19.2 60 236 20.5 60 290 22.8 60 333 24.3 55 367 27.9 S5 399 30.4 55 425 32.2 70 178 18.7 70 234 20.2 70 285 23.2 70 323 25.0 65 361 28.1 65 390 30.2 65 413 32.7 40 178 19.0 40 231 22.0 40 286 25.2 40 326 28.0 40 363 31.4 40 394 34.6 40 415 36.8 covan Table 3 Summary of Body Weight Data (9) ,CITY STUDY WITH PAGE 104-PWEEREFKLUODRIOEOTCATRANYE CHRSOUNLIPCONITCOXIACCIITDY POATNADSSCIAURMCINSOAGLETNIN RATS *DRAFT* ------------------------------------------------------------------------------------------ SEX: ----------------M-AL-E --------------- ----4----------5----------6--------- 2 3 2 5 20 20 GROUP: 1 DOSE: 0 0.5 PPM PPM PPM P-P-M ---------P-P-M---------------------------------------- W-E-E-K--U-N-I-T-S:------P-PM------------------------------------ a N 65 MEAN 448 S.D. 37.8 9 N 65 MEAN 464 S.D. 41.6 10 N 65 MEAN 481 S.D. 43.1 11 N 65 MEAN 499 S.D. 45.2 12 N 65 MEAN 509 S.D. 46.8 13 N 65 MEAN 517 S.D. 48.0 14 N 65 MEAN 528 S.D. 51.1 55 450 35.7 55 463 37.7 55 483 39.8 55 502 42.1 55 512 43.9 55 518 45.6 55 534 47.3 55 4143 35.9 55 4150 38.2 55 1473 40.7 55 479* 42.6 55 494 44.3 55 508 47.5 55 517 49.4 55 4485 3 .1 55 466 36.8 55 479 36.9 55 494 38.5 55 507 40.4 55 514 42.5 ss 527 44.4 65 432 34.2 65 446* 36.4 65 465 37.2 6!i* 474 38.5 65 485* 41.5 65 493* 43.2 65 S04* 46.1 40 436 40.8 40 446 41.0 40 466 42.2 40 473* 45.2 40 483* 46.4 40 491* 49.1 40 Sol*. so 4 covanc Table 3 summary of Body Weight Data (9) PAGE: IC roXICITY AND CARCINOGENICITY STUDY WITH IN RATS 104-WM DIETARY CHRON LPONIC ACID POTASSIU14 SALT ------------- PERIFLUOROOCTAKE SU *Dpbl"* ------------------------------- ------------------------------------------------------------- ---------------- -------------- .----MALE --------------5----- 6 SEx: --------------------------3 --- 4 1 2 2 5 20 20 PPM -------------------------- GROUP" DOSE: 0 0.5 PPM PPM PPM --------------------------------- WEEY UNITS: PPM ppm ------------------- ------------------------------------------ 60 40 is N 60 50 542 MEAN 535 50 531 49.0 50 535 45.5 513* 47.5 510* 51.2 S.D. 51.9 48.2 60 40 60 50 54s2o 55500 523* 51572*.3 16 NMEAN 549 5S3 46.5 49.3 SO.0 s.D. 53.5 49.9 60 40 60 so 50 55!0il 526* 52512*.9 17 MEAN 556 559 551 51.0 47.8 49.8 S.D. 5S.4 50.5 60 40 60 49 58502 58510 546* 54549*.2 21 N MEAN 580 594 57.5 53.2 57.2 62.1 57.5 60 40 S.D. so 50 567 566* 25 NMEAN 65099 49 621 63.0 611 61.0 604 . 56 7 62.9 65.8 S.D. 67.8 50 so 6@o 59,5* 40 595* 29 N 59 49 654 640 632 62.5 69 .0 69.8 MEAN S.D. 644 76.0 66.6 67.4 50 oio 40 620* 33 N 59 68439 66506 65638.8 61796*.6 15.1 MEAN 669 71.0 70.4 S.D. 79.5 covance Table 3 ot Body Weight Data (9) SummarY PAGE: C TOXICITY AN15 CARC3:NOGENICITY STUDY WITH 104-WEF,K DIETARY CHRONI, FONIC ACID POTAS.SLM SALT IN RATS -------------- PERPLUOROOCTANE SUL@ *DftAFT* -------------------------------- ---------------------------------------------------------------------------- -------tiALE -------------------------- 4 5 6 20 SEX: GROUP: 1 2 3 5 20 PPM ppm -------------------------------------- DOSE: 0 5 PPM ppm ------------ 0P.PM ------------------------- W EE X---U-NI-TS-,-. -----P-P-M ----------------------------- 40 --- 50 59 644* 3'7 NMEAN 69591 49 700 71.8 51) 68'7 73.8 fil 73 6 644* 84.7 81.8 S.D. 83.5 so 59 40 657 59 48 !)i2o 690 663 92.5 89.6 41 N 699 716 "078.2 7-7.6 MEAN S.D. 81.9 81.8 59 40 150 51) 6*76 674 45 N 59 48 735 721 708!2.3 100. 1 97.6 MEAN 715 95.8 87.8 80.4 40 S.D. so 50 58 691 699 49 N 58 48 759 740 726 88.9 108.3 104.3 MEAN 136 89.5 83.5 S.D. 91.2 4@8 57 40 712 513 N 58 MEAN 149 48 77 0 91.7 so 753 86.2 742 . 97 I 713 115.0 109.2 S.D. 103.5 Table 3 Summary of Body Weight Data 19) Covan 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PACE PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS *DRA17T* ------------------------------------------------------------------------------------------------------------------------- SEX: ----------------------------F-EK-A-LE- -------------------------------- GROUP: 1 2 3 4 s 6 DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------- 1 N 70 MEAN 154 S.D. 11.1 2 N MEAN 183 S.D. 14.6 3 N 70 MEAN 207 S.D. 18.6 4 N 70 KEAN 224 S.D. 22.1 5 N 65 MEAN 23) S.D. 23.6 6 N 65 MEAN 248 S.D. 25.3 7 N 65 MEAN 254 S.D. 27.7 60 154 10.4 60 180 15.0 60 204 17.4 60 222 21.2 55 236 22.9 55 250 25.0 55 257 27.1 60 iss 10.0 60 176 14.2 60 199* 15.7 60 217 18.9 55 232 20.1 55 245 21.6 55 251 20.0 60 154 10.1 60 ISO 13.2 60 204 is.s 60 218 19.1 ss 232 20.0 55 244 21.1 S5 250 21.7 70 153 11.1 70 177 13.5 70 199* 16.2 70 215* 11.4 6s 224* 17.4 65 233* 19.6 65 236* 20.1 40 iss 11.8 40 178 14.8 40 198* 15.8 40 214 18.8 40 22'1 19.4 40 235* 19.8 40 240* 18.8 Table 3 Summary of Body Weight Data (g) Covan, 104-WEEK DIETARY CRRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PAGE PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS *DRAFT* ------------------------------------------------------------------------------------------------------------------------- SEX: -----7---------------------F-E-M-AL-E--------------------------------- GROUP: 1 2 3 4 5 & DOSE: 0 0.5 2 5 20 20 WEEK UNITS: PPM PPM PPM PPM PPM PPM ------------------------------------------------------------------------------------------------------------------------- 8 N 65 MEAN 266 S.D. 30.0 9 N 65 MEAN 268 S.D. 31.4 10 N 65 MEAN 279 S.D. 33.5 11 N 6s MEAN 281 S.D. 33.8 12 N 65 MEAN 290 s.D. 37.0 13 N 65 MEAN 295 S.D. 38.9 14 N 6s MEAN 300 S.D. 40.1 55 269 28.1 55 273 30.2 55 283 31.7 55 291 33.1 55 292 34.5 55 296 3S.3 55 303 37.6 55 262 23.0 55 263 21.7 54 271 23.7 54 277 23.8 54 278 23.6 54 284 24.4 54 292 24.5 ss 263 24.4 55 264 25.3 5s 275 27.2 55 277 25.8 5S 279 27.5 55 285 29.1 55 292 31.7 65 247* 20.2 65 245* 22.0 GS 253* 22.4 65 258* 22.6 65 257* 21.1 65 261* 22.7 65 265* 23.3 40 250* 21.0 40 247* 22.1 40 254* 22.0 40 255* 22.6 40 259* 23.0 40 262* 23.2 40 266* 23.6 covance Table 3 ot Body W,Jght Data (9) Summary PAGE: 104-WEEK DIETARY CHRONI:C TOXICTY AND CARCINOGENICITY STUDY WITH 'FONIC ACID POTASSIUM SALT IN RNTS PERFLUOROOCTAM SUL *DPJKFT* --------------------------------------------------- -------------------------------------------------------------------------------------------------- --------------F-E-I"E---- SEX: ---------------- 3 4 5 6 20 GROUP: DOSE: 1 2 o.5 2 5 20 PPM ------------------------- PPM ------------------------ 0 ------P-P-M-- ------p-p-m---------pp-m-------------------- WEEK UNITS: PPM ----- --------------- so 60 40 269* so 249k49 298 266* 22.0 23.7 is 60 MEAN 302 304 36.7 2 6.4 33.3 S.D. 42.2 50 60 40 271* so 49 2;8 303 268* 23.1 24.3 16 N 60 MEAN 307 310 39.1 28.2 33.8 S.D. 43.9 49 so 60 40 273* 60 50 311 303 305 272* 23.6 25.0 17 N ME" 3 07 38.3 26.5 34.9 s.D. 43.9 49 so 60 40 280* 60 50 322 314 313 38.2 280* 24.1 26.5 21 Nmm 316 40.2 29.9 S.D. 44.8 !io 60 40 291* 50 49 324 32ps 29207*.8 29.6 25 N 60 MEAN 331 335 45.6 30.7 42.2 S.D. 50.9 498 50 6() 40 301* :z9 N 60 50 347 33 342 47.2 298* 30.2 32.5 MEAN S.D. 342 S2.8 50.0 36.1 49 60 40 312* 33 N 60 36s2o 34596 35436.8 31302*.8 36.8 MEAN S.D. 358 58.0 55.0 40.7 covanc Table 3 Sunnary of Body Weight Data (9) PAGE 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS *DRAFT* ------------------------------------------------------------------------------------------- SEX: ----------------------------F-EM-A-L-E 2 3 -------------------------------- 4 5 6 GROUP: 1 DOSE: 0 0.5 2 5 20 20 PPM PPM WEEK UNITS: PPM PPM PPM PPM --- ------------------------------------------- ------------------------------------------------- 37 N 60 MEAN 373 S.D. 64.8 41 N 60 MEAN 389 S.D. 69.4 45 N 60 MEAN 409 S.D. 73.3 49 N 60 MEAN 422 S.D. 74.5 53 N 60 MEAN 427 S.D. 78.8 49 375 56.7 49 393 62.4 48 413 63.6 48 424 66.3 47 435 71.8 49 371 43.4 49 385 50.0 49 396 59.8 47 41S 55.7 47 428 61.2 49 365 47.3 49 378 49.8 49 394 51.1 49 407 55.1 48 422 56.5 60 319* 36.6 59 328* 38.3 59 339* 41.0 59 351* 45.5 59 358* 48.3 40 321* 39.8 40 329* 46.5 39 346* 48.0 39 356* 50.0 39 366* 52.6 covan Swmmry Table 4 of Bocly Wsight change Data to) 104-WEEK DIETARY pEpyLUoRoocTANE CARCINOGENICITY STUDY WITH PAG CHRONIC SULPONIC TOAXCIICD:ETPYOTAASN"DSIUM SALT IN RATS *DRAFT ---------------------------- ----------------------------------------------------------------------------------------------------- SEX: -------------- GROUP: 1 2 0 0.5 3 MALE ----4----------5-----------6 -------2 5 2PP0M 2PP0M DOSE: WEEK UNITS: PPM PPM --P-PM---------P-P-M--------------------------------------------------------------- -------------------------------------- 1-2 2-3 3-4 4 -5 5-6 6-7 7-8 N MEAN S.D. N MEAN S.D. N MEAN S.D. N KEAN S.D. N MEAN S.D. N MEAN s.D. N MEAN S.D. 70 58 5.8 70 50 6.5 70 47 6.8 65 34 7.6 65 32 6.2 65 20 6.5 6S 29 5.8 60 59 5.6 60 53* 6.2 60 42* 8.0 55 38* 6.3 55 32 7.0 55 26* 5.9 55 22* 6.7 60 58 5.2 60 48 6.2 60 43* 7.6 55 38* 6.9 55 30 5.7 55 21 6.3 55 25* 7.2 60 57 4.4 60 55* 7.5 60 43* 6.8 55 34 8.2 55 32 5.3 55 26* 5.8 55 23* 6.7 70 57 5.9 70 50 6.2 70 38* 6.6 65 37 5.6 65 29 5.1 65 23 7.5 65 20* 5.5 40 53* 6.4 40 56* 7.2 40 40* 7.3 40 37 5.8 40 30 6.0 40 21 5.3 40 21* 10.4 Coval Table 4 Summary of Body weight change Data (9) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PAG PERFLUOROOCTAKE SULFONIC ACID POTASSIUM SALT IN RATS *DRAFT* -------------------------------------------------------------------------------------------------------- SEX: -------------------------3----KA-L-E------4----------5-----------6 -------- GROUP: 1 DOSE: 0 2 0.5 2 5 20 20 W-E-E-K---UN-I-T-S-: -----P-PM----------PP-M---------PP-M---------p-pm---------P-PM----------PP-M------------------------------------------------- 8-9 N MEAN S.D. 9-10 N MEAN S.D. 10-11 N MEAN S.D. 11-12 N MEAN S.D. 12-13 N MEAN S.D. 13-14 N MEAN S.D. 14-15 N MEAN S.D. 65 17 11.0 65 16 5.7 65 is 5.3 65 10 8.2 65 9 5.8 65 11 5.4 60 7 5.8 55 13 6.1 55 20* 4.7 55 19 5.1 55 10 4.2 55 6* 5.5 55 16* 4.7 50 6 6.5 55 7* 5.8 55 23* 6.5 55 6* 6.9 ss 15* 9.3 55 14* 6.8 55 9 4.4 so 9 4.7 55 18 4.7 55 13* 4.0 55 15* 6.8 55 13* 6.0 55 7 5.1 55 13 4.3 50 ll* 5.7 65 13 . 79 65 20* 5.3 65 9* 6.5 65 10 5.6 65 8 4.7 65 11 7.1 60 8 4.5 40 9* 9.0 40 20* 5.0 40 8* 5.8 40 10 4.3 40 8 5.4 40 10 5.8 40 9 5.1 Covai Table 4 Summary of Body Weight Change Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PAG PE"LUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS *DRAFT* ---------------------------------------------------------------------------------------------------------- SEX: GROUP: -----1 ----------2----------3----MA-L-E------4----------5----------6--------- DOSE: 0 0.5 2 5 20 20 W-E-E-K---UN-I-T-S-: -----P-PM---------P-P-M ---------pp-m---------PP-M---------P-PM----------pp-m-------------------------------------------------- 15-16 16-17 N MEAN S.D. N MEAN S.D. 17-21 N MEAN S.D. 21-25 N MEAN S.D. 2S-29 N MEAN S.D. 29-33 N MEAN S.D. 33-37 N MEAN S.D. 60 14 4.7 60 7 5.3 60 25 12.0 59 28 9.9 59 35 13.0 59 26 10.2 59 21 10.3 so ll* S.6 50 7 4.6 49 34* 10.5 49 27 12.9 49 34 13.5 49 29 10.7 49 17 23.1 50 ll* 4.7 50 9 4.6 50 30* 11.3 50 30 10.5 so 29* 10.3 50 25 8.9 50 21 13.0 50 14 3.9 50 8 4.2 50 23 11.6 so 23 9.9 50 28* 9.6 50 20* 12.1 so 23 9.8 60 10*. 46 60 3* 5.5 60 20 11.4 60 21* 9.9 60 28* 11.7 60 24 12.4 59 26 11.9 40 8* 4.6 40 4* 4.7 40 22 10.4 40 22* 12.1 40 30 7.7 40 25 9.7 40 24 11.8 covanc4 Table 4 Sumary of BodY WGight change Data (g) 104-wEBY, DIETARY PERFLUOROOCTANE :C TOXICITY AND CARCINOGENICITY STUDY WITH PAGE: CHRON] SIUM SALT IN RATS SULFONIC ACID POTAS *DP-bM* ------------------------------- ------------------------------------------------------------------------------------------ ---------------M-AL-E 6 GROSUEPX:: -----1----------2- 3 4 5 20 20 0 0.5 2 ppm PIPH PPM PPM -------------------------- WEEK UNDIOTSES:: PPM PPM --------- ----------------------------------- ------------------------------------------- 37-41 N MEAN S.D. 41-45 N MEAN S.D. 45-49 N MEAN S.D. 49-53 N MEAN S.D. 1-53 N MEAN S.D. 59 8 12.5 59 16 19.8 58 20 15.6 58 13 15.9 58 570 100.0 48 13 11.9 48 19 10.2 48 24 8.9 48 11 IS.0 48 593 91.6 so 15* 10.6 50 198.6 50 19 9.7 50 13 8.9 50 573 83.0 so 15* 14.2 50 19 12 4 50 17 12.9 48 12 28.3 428 56 96.0 59 19* 11.7 59 13 14.1. se 21. 13 8 57 14 13.2 57 535 112.8 40 13 11.4 40 16 10.9 40 24 9.6 40 15 10.3 40 534 104.9 covanc Table 4 Summary of Body Weight change Data (9) PAGE 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH pERFLUoROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS *DRAFT* ----------------------------------------------------------------------------------------- FEMALE ------------------------------- SEX: GROUP: -----1----------2----------3------- 4 5 5 20 6 20 DOSE: 0 0.5 2 PPM PPM PPM ppm --------P--P-M -------------------------------------- W-E-E-K--U-N-I-T-S:------P-PM-------------------------------------------------- 1-2 2-3 3-4 4-5 5-6 6-7 7-8 N MEAN S.D. N MEAN s.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. 70 29 6.7 70 24 7.0 70 17 7.8 65 14 7.2 65 11 6.5 65 6 5.8 65 13 6.8 60 26* 7.5 60 24 6.6 60 18 7.0 55 14 7.9 55 14 6.3 55 6 7.4 55 12 5.0 60 21* 6.9 60 23 6.3 60 18 6.9 55 15 8.2 55 13 5.4 55 6 5.9 55 11 7.4 60 26* 6.3 60 24 6.5 60 14 7.3 55 is 7.8 55 12 6.9 55 6 7.3 55 13 6.0 70 25* 7.2 70 22 7.0 70 16 7.6 65 9* 7.4 65 9 5.7 65 3* 5.0 65 11 4.9 40 23* 6.1 40 20* 7.4 40 16 8.2 40 13 7.2 40 8* 5.8 40 5 6.0 40 10 5.1 covance TablO 4 Summary Ot Body weight Change Data (9) PAGE: 104-WEEK DIOECTTAARNYE pEpYLUORO EC TOXICITY AND CARCINOGENICITY STUDY wiTH CSHURLOFNO]NIC ACID.POTAS* SIUM SALT IN RATS ------------------------ DRAFT --------------------- ----------------------------------------------------------- ---------------- ---------------------------- ---FEMALE ---- 5 6 SEX: -----1----------2----------3---- 45 20 20 --------- PPM ----------------------------- GROUP: 0 0.5 pp2m PPM ppm ------------- DOSE: ppm ------------------------- WEEK UNITS: --P-P-K---------- 40 --------------- 55 65 -3* 5S ss 1 -2 4.6 2 4.6 8-9 N 65 4 6.2 MEAN 2 49 S.D. 6.0 6.2 5s 65 40 8* 7* 55 54 8* 11 . 6.6 5.5 9-10 N 65 10 MEAN 11 S.8 68 S.D. 6.3 6.8 65 40 ;s 5* 0 558* S4 6* 2 5.6 4.9 10-11 N 65 14EAN 2 5.7 5.8 5.7 s.D. 5.1 155 65 40 4* 65 55 54 1* 1* 2* -1 4.6 5.8 11-12 N MEAN 9 6.6 5.7 6.0 S.D. 6.3 55 65 40 3 65 55 54 7 6 3 5.3 5.0 12-13 NMEAN 5 45 .3 5.9 4.8 S.D. 7.0 55 65 40 5 13-14 N 65 55 S4 7 7 4 4.3 5.1 MEAN 57.0 5.3 85*.1 5.5 0 40 S.D. 60 so 49 3 2 50 6 2 4 1. 3.8 6.4 54 14-15 NMEAN 4 65 6.0 S.D. 5.8 covance Tabl8 4 Sumary of Body W,,ightChangs Data (9) PAGE: D CARCINOGENICITY STUDY WITH 104_WF .Er DIETARY CHRONIC TOXICITY AN slUM SALT IN RATS OCTANE SULFONIC ACID POTAS --- PERFLUORO ------------------------------------------ ----------------------------------------------------------------------------- -------F-E14ALE -------------5-----------6- SEX: GROUP: 1 DOSE: 0 2 3 45 20 2PP0M -------------------------------------- 0.5 2 ppm PPM PP-M---------------------------------------- WEEK UNITS: --P-PM----------PP-M------------- --------------- so 60 40 3 so 919 4 5 35.3 4.1 15-16 NMEAN 604 6 5.3 5.7 5.6 S.D. 4.4 60* 40 60 50 494* 503 4 2 5.1 16-i'l NMEAN 0 1 7.0 6.2 4.5 S.D. 6.5 4.8 50.1 60 40 7 8 17-21 N 609 5101 411910.6 8.9 6.6 6.9 MEAN 8.1 6.7 40 S.D. 49 so 60 9* 11 21-25 NMEAN 6105 50 io* 16 . 9.0 5.5 13 11.3 93 9.9 S.D. 9.2 50 60 40 9 so 49 is 14 . 98.0 'J.6 25-29 N 60 MF" 12 129.8 11.9 9 9 S.D. 7.8 d9 6() 40 12 29 -33 N 60 16 MEAN sios 4i19s3.8 1130.0 i:9z.7 9.6 13.1 S.D. jo.8 60* 40 60 49 4194 4192 9 9 10.S 33-31 NMEAN 15 15 9.9 9.9 8.2 S.D. 12.9 12.2 covance Tabls 4 surmary of Body Weight change Data (a) D CARCINOGENICITY STUDY WITH PAGE: 104-WEEK IDILrrARYCHRONIC TOXICITY AN SlUm SALT IN RATS ------------ PERFLUORO OCTANE SULIPONIC ACID*DPFO.TAAnS* --------------------------------------- ------------------------------------------ ----------------------------- ----------------------------- ---------------FE-M-ALE--- 5 6 3 4 20 20 ------------ SEX: -----1----------2- 2 5 ppm -------p-p-m------------------------------- GRDOOUSPE':- 0 0.5 ppm PPM -P-P-M -------------------------- WEEK UNITS: --p-p-m --------------------- 40 --------------- 49 59 8* 49 13. io* 16.7 31-41 N 60 49 is 14 a9 9.3 MSE.ADN. 1l6o.s 13.7 18.0 49 59 3194* 48 49 17 10.1 4 1-45 N 6109 . 18 1313.2 12.4 7.5 MSE.ADN. 9 a 13.0 47 49 5192 3109 60 48 13 13 9.3 8.9 45-49 N MEAN 13 11 9.0 8.8 11.6 S.D. 16.3 S9 39 48 10 47 47 14* 7 7.3 419-53 N 60 ll:& 13* 10.4 10.5 MSE.ADN. 250.8 10.6 1417-1 48 25096 23191* 1-53 N 60 47 282 274 268 . 51 4 46.0 48.6 MEAN 273 66.7 57.9 S.D. 73.5 covance TablO 5 Data (9) suvmary Of pood COnsumPtion PAGE* ) CARCINOGENICITY STUDY "ITH 104-WEEK D T.ETTAANREY CHRONIC SULFONIC TOXICITY AM ACID.POTASS luM SALT IN RATS ------------------------ PEPYLUOROOC DRAFT* ------------------------- ------------------------------------------------------- ----------------- -------------- ---MALE ----------------- 6 GROSUEPX:: -----1---------0-2-5---------3-2 --- 4 20 5 20 PPM --------------------------- ppm ------p-p-m----------------------------- I)OSE* 0 ppm ppm ------------- WEEK UNITS: -p-pm--------------------------- ---------------- 69 39 56 61) 59 164 Ise* 166 14.2 1 N 69 165 165 13.2 11.6 ME" 16'7 13.3 11.5 14.1 37 s.D. 54 69 172 2 N 62 54 173 1113 172 167* 13.8 17.2 13.4 MEAN 17'7 13.9 12.9 13.9 38 S.D. 56 57 17708 181 3 N 68 184 MEAN 52 183 13.0 IBI 14.1 182 13.4 15.2 16.0 S.D. 12.9 48 62 13884 60 47 49 184 17 7* 176* 14.7 17.4 4 NMSE.ADN. 19116.5 is1s3.7 19.3 15.0 64, 40 62 50 18560 18495 183 18178.7 5 N MEAN 191 193 16.7 16.3 15.6 S.D. 16.1 16.1 65 38 43 46 49 1816 lei* 185 17.6 6 N 56 192 191 187 6.4 15.9 MEAN S.D. 15.1 17.3 15.9 60 37 47* 45 183* 184 59 42 195 183 187 17.2 15.4 19.9 7 NMEAN 193 18.3 ia.8 S.D. 15.6 covance 3 Table 5 surmary oi pood ConsumPtion Data (9) 104-WEEK DIETARY PMWLUOROOCTANE PAGE: CARCINOGENICITY STUDY WITH : TOXICITY AND CHRONIC ACID POTASSIUM SALT IN RATS ----------- SULPONIC *DltPM* ---------------------------- ------------------------------------------------------------------ ----------------- ------------- - --MALE --------------5------ 6 SEX: ---------------2-----------3 --- 4 5 1 2 20 20 ppm --------------------- GROUP: DOSE: 0 0.5 PPM --------P-PM------------------------------------ PPM --------p-p-m -------------- WEEK UNITS: PPM -------------------------------- 60 34 54 is 4'j@ 43 1'78* 181 iss 19.7 8 NMEAN 189 193 181@ 17.7 15.3 18.0 s.D. 19.2 17.9 60 32 59 47 183 19460 175* 18127.8 9 NMP" 190 190 16.9 17.4 15.5 17.0 S.D. ja.o 59 38 59 48 46 18438 177* 18159.1 10 NMEAN 188 189 18187.2 15.6 15.1 S.D. 18.0 17.6 59 38 60 46 4@8 i4s5s 179* 18128.9 11 N MEAN lee 189 1E13 . 17 7 15.1 17.8 S.D. 16.8 18.3 61 39 51 !52 4!) 185 iso 178* 12 N 18597 189 184 20.5 16.7 21.1 18.6 MEAN 18.5 S.D. 20.1 54 52 18603 13778 60 52 187 187 185 18.8 19.6 21.0 13 N MEAN 185 16.8 19.2 17.4 38 S.D. 59 49 48 186 188 45 la12 17.0 57 178 . 16 0 184 20.8 14 N MEAN 184 17.4 19.0 18.9 S.D. covance 3 Tabl8 5 summary ot pood ConsumPtion Data @9) PAGE: 3 CARCINOGENICITY STUDY '41TH L,rARy CHRON3:C TOXICITY AND U,4 SALT IN RATS 104-WEEK DI SULPONIC ACID POTASS PERFLUOROOCTANE *DRAFT* ----------------------------------------------------- 7 ---------------------------------------------------------------------------- )"E --------------------- 6 5 -------------- SEX: ---- 2 3 4 5 20 20 --- PPN -------------------------------------- GROUP: 1 DOSE: 0 0.5 pp2m PPM ------P-PM---------------- WEEK UNITS, PPM PPM --------------------------- ------------------------------- 56 40 45 185 se 41 19417 181 18121.1 is.7 15 N 190 188 16.0 14.1 MEAN s.D. 16.1 18.8 59 38 41@ 49 183 183 16 N 57 49 191 192. 191 19.5 17.6 18.6 MEAN 190 16.5 17.3 14 0 57 38 S.D. 49 41 184 184 20 N 58 46 195 194 188 . 20 9 18.3 21.3 MEAN 192 17.9 16.1 39 S.D. 19.1 so 60 118 58 48 19429 184 112708.3 21.5 24 N 184 188 18.6 20.2 HEM S.D. 20.1 20.1 59 36 418 46 iso iso 28 N 56 18456 as 181L le-I 11.8 16.3 MEAN 183 15.6 1.5 20.4 35 S.D. 48 4-7 18592 179 32 N 58 117 MEAN 45 184 14.4 184 15.3 182 17.4 19.3 JS.7 39 S.D. 16.8 50 se lal 36 N 59 183 48 181 . 50 181 16.6 183 19.9 183 21.1 23.0 MEAN 11.9 28 3 S.D. covance TablO 5 sunaary of Food Consugption Data @9) PAGE: ) CARCINOGENICITY smy WITH 104-WEEK D :ELPTARYCHSRUOLNFIOCNICTOXAICCIIDTY POTAANSSIUM SALT IN RATS ------------------- PERFLUOROOCTANE *DRAIFT* ----------------------------- -------------- -------------------------------------------------------- ------------- --- -----MALE ------------------------ 5 6 SEX*. 3 4 20 20 ---------- 2 5 PPM ----------------------------- GROUP: 0 o.5 2 PPM PPM ------P-P-M----------------- DOSE: PPM p-p-m--------------------------- WEEK --U-N-IT-S-:---------------------- 58 39 46 Ass is48.7 197 18222.7 40 N 18584 184 186 16.2 20 3 22.5 MEAN S.D. 19.1 19.1 47, 55 i3s9$ 58 48 14159 186 18240.7 23.9 44 N MEAN 182 iss1.0 814.7 20 0 24.9 2 55 37 185 S.D. 48 148 185 184 18.3 48 N 57 184 191 186 18 8 18.3 20.8 MEAN 20.2 18.5 54 37 44 SN.D. 54 18456 184168 l2a'l1.5 17282.4 17290.9 176 7.3 S2 MEAN 27.6 17.8 S.D. cov Tabl* f Food ConsuMtion Data (9) surmary p 104-WE,1U3OXRODOICETTAANREY PEWL CSHURLOFNOINCIC TOXICITR AMD CARCINOGENICITY STUD' WITH ACID POTASSIUM SALT IN RATS --------------- *DPun* ------------------------- --------------- -------------------------------------------------------------------------------- FE14ALE ---------------5 6 SEX: ------------ 2 3 4 20 5 20 PPM --------------------------------- GROUP: 1 DOSE: 0 0.5 PP2M -P-P-M ----------PP-M----------------- WEEK UNITS: -----p-pm----------P-PM----------------------- 62 35 ------------ 48 55 124 125 1 N 55 MEHN 129 55 120* 12.0 123 14.3 130 13.5 11-2 12.4 34 S.D. 11.9 62 51 41 130* 126 2 N 62 48 133 131* 136 ig-6 13.8 12.7 MEAN 140 16.5 11.8 33 S.D. 16.9 55 51 129 54 49 14501 141 13114*.7 13.3 3 N 144 142 11.6 14.9 MEAN 16.8 17.0 58 33 S.D. 41 45 126* 128 4 N 41 44 137 142 138 18.3 12.5 14.0 MEAN 140 15.9 11.5 20.1 55 33 S.D. 43 45 135* 130* 5 N 50 48 146 144 144 19.3 14.0 11.4 MEAN 146 18.5 17.6 14.2 31 s.D. 42 ss 124* 45 40 35 137 134 124* 11.4 13.1 6 N 138 142 20.3 Il.3 WIAN S.D. 18.6 23.3 46 53 12363* 54 49 4S 133 136 126* JO.7 12.0 1 N 138 142 12.2 16.3 MEAN 17.0 S.D. coi Table 5 f Food consumption Data (9) sumary p 104-WEF.r, DIETARY pEpyLUOROOCTANE VV AND CARCINOGENICITY STUDY WITH ARON3:C TOX'C,'OTASSIU14 SALT IN RATS C'LI20NIC ACID P -------------- SU *DRAFT* --------------------------- ------------------ ----------------------------------------------------------- ------------------ -------------- -------FEMALE 5 6 SEX: ----------------2--------- 3 4 20 20 s ppm --------------------------- GROUP: 1 0.5 2 ppm PPM ---------------- DOSE: 0 PPM ppm ----------------------- WEEK UNITS. ppm --------------------------- ------------------------ 54 31 38 43 42 123* 121* 135 12.6 8 N 46 138 136 15.5 14.7 mmw 138 15.9 11.3 S.D. is.5 46 56 36 125* 55 48 13421 134 12122*.6 17.5 9 N MEAN 140 13S 15.2 11.0 17.3 18.8 45 53 34 125* S.D. 53 45 39 135 124* 14.4 134 io.0 10 N MEAN 141 135 16.0 Ij.0 13.8 S.D. 18.2 55 38 11 N S6 13542 13436 1341486.0 12120*.2 121S4*.8 138 13.4 S.D. 21.7 15.5 so 35 52 43 13379 13468 12j7g*.s 12131*.1 12 MEAN 138 133 15.7 17.6 21.5 S.D. 20.1 49 59 123S5 56 46 14371 131 12128*.4 16.8 13 N MEAN 133 135 17.7 13.1 20.1 S.D. 17.7 40 54 12322* 53 40 13337 125 11191*.3 8.5 14 NMEAN 131 129 js.O 13.4 11.3 S.D. 20.4 c Table 5 Summary of Food Consumption Data (g) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY PERFLUORO(>CTANE SULFONIC ACID POTASSIUM *DFL%M* SALT IN RATS ---------- --------------------------------------------------------------- WITH ------------ FE m E ------------------------------- SEX: -------------------------------- 2 3 4 5 6 GROUP: 1 DOSE: 0 0.5 2 5 20 20 PPM PPM WEEK UNITS: PPM PPM PPM PPM - ------------------------------------------------- ----------------------------------------------- ------------- 15 N MEAN s.D. 16 N MEAN S.D. 20 N MEAN S.D. 24 N KEAN S.D. 28 N MEAN S.D. 32 N MEAN S.D. 36 N MEAN S.D. 53 133 17.1 54 133 19.4 50 139 19.0 53 134 18.9 S6 132 15.7 58 136 18.4 5S 135 17.9 49 134 15.9 44 135 18.1 46 134 16.0 44 132 18.7 48 133 24.7 47 134 26.5 49 135 20.9 43 135 14.9 41 136 15.3 42 136 16.2 44, 136 17.6 45 141 20.5 45 139 15.5 46 137 15.3 45 133 16.2 45 133 15.6 44 138 18.8 47 132 20.0 48 132 18.7 45 134 17.9 47 133 17.8 57 124* 13.6 56 124* 12.2 49 128* 12.8 54 123* 14.0 56 122* 20.4 58 124* 17.4 58 124* 16.1 39 125 17.7 34 124 15.0 28 131 14.2 32 122* 13.3 40 121* 18.9 40 127 17.7 39 123* 14.9 Ca Table 5 SU=Ray of Food Consumption Data (9) V=y rY AND CARCINOGENICITY 104-1@rEEY,DIL'RARY CHRONIC TOXICI' SIU14 SALT IN RATS PMUFLUOROOCTANE SULFONIC ACID*DRPAOFTATS* p 141TH --------------------- ------------------------------------------------------------ ---------- --- ------ SEX. -----------------------------3---FEMALE ---4-----------5 --- 6 20 GROUP: * I 2 2 5 2PP0M ppm 7 ------------------------- DOSE?.: 0 o.5 ppm Ppm PPM -------------------------- WEEY, UNITS ppm -------------------------------------- ------------------------ 56 38 56 44 14428 13487 135* 13104.7 40 NMEAN 139 139 20.6 17.6 20.7 S.D. 20.3 15.6 53 38 56 45 45 13474 126* 12185.9 44 N MEAN 137 134 137 18.6 20.7 17.1 s.D. 17.8 19.3 47 47 57 38 130 133 141 13.3 48 NMEAN 13548 13438 14126.6 17.8 20.0 s.D. 23.6 19.5 46 45 57 38 133 133 139 14.0 52 NMEAN 13573 13445 14119.8 22.2 21.9 S.D. 24.0 20.5 c Summary Table 6 Test Material Consumption Data of (mg/kg/day) 104-WEEK DIETARY PERFLUOROOCTANE CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH IN RATS ------------------------------ SULFONIC ACID POTASSIUM SALT - - --------------------------------------------------------------------------------------------------------------FE-MALE -----------5 --MALE -------------------- 6 2 3 4 s 20 ------------------------3 4 5 20 0.5 2 PPN PPN SEX: GROUP: 2 2 5 20 PPN PPN ---------PP-N----------------------------- DOSE: 0.5 PPN ---P-PM----------P-PM----------p-p-m--------------------------- 62 W-E-ER-,---U-NI-T-S--------------- 39 55 48 55 0.559 2.1 60 59 69 2.205 2.336 0.052 0.213 0.020 0 046 0. 1 N MEAN 56 0.057 0.226 0.570 0.041 0.156 0.170 0.004 62 S.D. 0.004 0.015 51 37 48 0.506 1. 2 N 54 0.047 58 0.189 54 0.471 0.031 69 1.846 0.115 J.907 o.118 0.050 0.005 0 200 0.*019 0.055 0. 57 MEAN S.D. 0.003 0.012 38 49 5101.194 550 ..481 1. 56 57 70 1.675 1.701 0.048 0.026 0 038 0. 3 N MEAN 52 0.042 0.169 0.420 0.026 o.097 0.097 0.003 58 S.D. 0.002 0.010 38 44 410.180 450.4q4t9o 1 49 48 62 1.477 1.531 0.043 0.018 0.0 0 4 N MEAN 47 0.039 0.151 0.364 o.020 0.081 0.086 0.003 55 S.D. 0.002 0.011 40 48 43 415 0.-431 1 5 N so 0.036 so 0.139 49 0.346 0.019 64 1.394 0.072 1.409 0.073 0.043 0.003 0.170 0.011 0.036 0 55 MEAN S.D. 0.002 0.007 38 40 3S 162 0 3' 90 1 6 N MEAN 43 0.033 46 0.132 49 0.325 0.017 65 1.287 0.065 1.307 0.068 0.040 0.005 0.157 0.019 0.* 034 0 53 S.D. 0.002 0.007 49 45 46 0.382 1 47 45 60 1.241 37 1.233 0.038 0.148 0.010 0.028 7 N MEAN 42 0.032 0.122 0.010 0.311 0.016 0.061 0.078 0.003 S.D. 0.002 ca Table 6 Consulaption Data (mg/kg/day) summary of Test Material p 104-WEEr DIETARY ppRFLUOROOCTANE HRONIC TOXICITY AND CARC3:1;OGENICITY STUDY WITH C SULPONIC ACID -P-O-T-AS-S-I-UM----SA-L-T--I-N--R-A-TS--------------------------------- ------------- --------------------------------------------------- -------------------------F-EKA] IE ----------5 - ---------------------------MALE --------------------------6 2 3 4 5 20 sm 3 4 5 20 0.5 2 ppm ppm GROUP: 2 2 5 20 PPM PPM PPM -------------------------------------- DOSE. : o.5 PPM PPM ppm ------------ ----------------- WEEK UNITS ----P-PM-------------------------------- 42 54 ----------- 60 34 38 0.036 43 0.148 .368 1.4 0.1 45 47 43 0.293, 1.161 1.178 o.002 0.011 0.03@O 8 N MEAN o.030 0.121 o.012 0.093 0.106 46 56 S.D. 0.002 0.007 60 32 48 41o1.143 0.355 1. 51 46 1.108 1.135 0.035 o.023 0.027 0. 9 N MEAN 47 0.029 0.001 0.113 o.006 o.288 0.016 0.076 0.063 o.002 38 45 319 4!5 . 53 0 355 1. S.D. 46 48 S9 1.074 1.130 0.033 0 .138 0.010 0 .033 0. 10 N 48 o.113 0.270 0.080 o.002 MEAN 0.028 0.006 0.017 0.060 o.002 46 48 5S1. S.D. S9 38 520.033 0.136 0.346 0. 11 N 46 48 0.108 45 0.266 1.056 1.086 0.071 0.003 0.011 0.029 MEAN 0.027 0.007 o.014 0.060 4is 58 S.D. 0.002 61 39 43 0.032 390 .137 0 '344 1 0 52 49 j.055 1.050 0.003 o.015 0.'040 12 N MEAN 51 0.026 o.002 0.106 0.009 0.262 0.016 0.101 0.075 47 49 59 46 0.324 1 s.D. 52 63 37 1.024 0.032 0.129 0.026 0 13 N 52 o.025 54 0.105 0.256 1.029 0.093 0.080 0.003 0.011 MEAN o.002 0.006 0.017 40 . 51t 38 40 3'7 0.130 0 .310 1 S.D. 48 45 57 1.007 1.043 0.030 0.012 0 031 0 14 N 49 0.025 0.102 0.248 0.073 o.104 o.004 MEAN o.007 0.018 S.D. o.002 cov Table 6 Data (mg/kg/day) sumary Of Test Material ConsuPtiOn p micilm S-MY WI'M 104-WEEKLUORDOIOECTTAARNEY TOXICITV AND CARC114OG SALT --IN RATS ---------------------------- C"SRUOLNFIOCNIC ACID -P-O-TA-S-SI-U-M---------------- PERF - FEMM 3------------- --------------- ----- ------ 4 5 -MALF 6 2 32 5 20 SEX: ----------------3---------- 4 20 20 o.5 ppm ppm PPM ----------- GROUP* 02.5 2 5 ppm PPM ----------------------- ppm ------------------------------ DOSE* wep,y, UNITS* ppm ---P-PM----------P-P-M-------------- 4-.1 45 51 1.33 ------------------------ 41 45 56J.010 401.032 4900..000341 0-.011219 00..301283 o.1 15 N MEAN 41 ().02S 0.002 o.102 0.006 0.250 0.013 0.074 0.069 38 44 0.031 4'1 D.129 45 0 ..315 56 1.31 0.1 s.D. 49 59 1.010 0.016 0 027 48 1.006 0.004 16 N MEAN 49 0.025 0.002 0.,Ioo 0.006 o.241 o.011 0.084 0.067 380 46 42 124 44 o.316 49 1.3 o.1 S.D. 49 41 57 0.969 910 0.030 0.003 00..012 o.029 20 N MEAN 46 0.024 0.001 o.096 0.007 0.234 0.020 0.065 o.'013 39 44 44 0 120 54 41 1. 0.:288 0. S.D. 49 so 600 904 0.904 o.028 0.004 0.'015 o.024 24 N MEAN 48 o.022 0.002 o.090 0.007 o.219 0.018 0.- 070 0.084 36 48 41a 56 45 279 1. 121 0. S.D. 46 59 0.872 o.028 00.-02 2 00-025 46 48 0.206 o.875 o.069 0.005 28 N o.021 0.083 0.016 ().082 45 58 HEM o.002 0.006 35 41 45 0 112 0. 273 I. S.D. 41 S9 0.831 o.027 0.013 o.o2g 0. 45 48 0.199 o.850 o.059 0.006 32 N MEAN o.019 o.080 o.006 o.012 0.083 46 4k7 581 S.D. 0.001 so 39 49 0.026 06 .@261 00 .'023 0 so 50 c).81*7 805 o.003 0.*01102 36 N 48 0.019 0.016 o.195 0.021 o.084 00-.046 MEAN 0.003 0.008 S.D. Table 6 Summary of Test Material Consumption Data (Mg/kg/d4tY) 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------- --------------------------- -- ----------------------FE-MALE ----------5 --------------------------MA-LE 5 6 2 4 5 20 SEX: GROUP: 2 3 4 20 20 0.5 2 PPM 2 5 PPM PPM WEEK UNDOISTES:: PPM0.5 PPM PPM PPM PPM PPM ------------- ---------------------- 48 47 56. 40 N 46 48 48 sa 390.794 440.025 0.106 0.263 1 0.018 0.076 0.196 0.812 0.090 0.052 0.003 0.014 0.025 0 MEAN S.D. 0.002 0.005 0.020 53 44 N 49 47 55 39 45 45 47 0.098 0.244 1 48 0.018 0.074 0.188 0.786 0.801 0.055 0.024 0.003 0.012 0.027 0 MSE.ADN. 0.002 0.006 0.017 0.069 57 48 N 48 47 55 37 48 0.023 47 0.099 47 0.247 1 48 0.018 0.072 0.184 0.765 0.780 0.049 0.003 0.013 0.020 0 MEAN S.D. 0.002 0.007 0.017 0.080 46 45 57 1 52 N 46 48 44 54 37 4S 0.022 @0.095 0.237 0.071 0.174 0.714 0.732 0.012 0.028 0 MEAN 0.017 0.006 0.025 0.082 0.053 0.003 S.D. 0.002 1.146 0.034 0.139 0.348 1 1-52 MEAN 0.028 0.115 0.284 1.147 104-WEEK Table 7 Summary of clinical Hematology Data Males Week 4 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ RBC X101/,uL -------- HGB G/DL -------- HCT % -------- mcv MCH FL PG -------- -------- MCHC PLT % X101/,uL -------- -------- 0 MEAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN s.D. N 20.0 MEAN S.D. N 7.47 .459 10 7.22 .414 10 7.54 s99 10 7.43 .622 10 7.30 .234 10 15.2 .81 10 14.7 .77 10 15.3 1.09 10 15.4 1.04 10 15.0 .44 10 44.8 2.35 10 43.4 2.43 10 45.1 3.39 10 45.0 3.32 10 44.2 1.56 10 60.0 l.s6 10 60.2 1.69 10 59.9 1.65 10 60.7 1.97 10 60.6 1.40 10 20.3 .73 10 20.4 .67 10 20.4 .85 10 20.7 .68 10 20.6 .45 10 33.9 .59 10 33.9 .36 10 34.0 .61 10 34.1 .41 10 33.9 .37 10 1048 107.7 10 1082 111.4 10 1028 68.7 10 1013 118.6 10 1051 118.1 10 104-WEEK Table 7 summary of clinical Hematology Data males Week 4 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PPOS; T-6295) IN RATS DOSE ppm T-6295 ------ WBC X101/,uL -------- N-SEG X101/,uL -------- LYMPH X10'/14L -------- MONO X103/,uL -------- EOSIN X101/,uL -------- BASO X10'/,uL -------- N-SEG% -------- LYMPH% -------- MONO% -------- EOSIN% -------- 0 MEAN S.D. N 8.6 2.03 10 .7 7.2 .30 1.92 10 10 .6 .1 .0 9 83 7 1 .16 .06 .00 3.5 5.3 2.2 10 10 10 10 10 10 10 0.5 MEAN 9.3 1.2 7.4 .6 .1 .0 12 82 6 1 S.D. 2.54 1.32 1.29 .31 .06 .00 7.6 9.1 2.5 N 10 10 10 10 10 10 10 10 10 10 2.0 MEAN 9.3 S.D. 2.30 N 10 .7 8.0 .6 .1 .0 7 86 6 1 .23 1.94 .26 .06 .00 1.4 3.4 2.3 10 10 10 10 10 10 10 10 10 5.0 20.0 MEAN S.D. N MEAN S.D. N 7.2 1.15 10 8.4 2.87 10 .6 6.1 .21 .99 10 10 .8 6.8 .46 2.19 10 10 .4 .1 .0 8 85 6 2 .24 .15 .00 2.3 6.5 2.9 2 10 10 10 10 10 10 10 .6 .1 .0 10 83 6 1 .38 .07 .00 2.8 4.8 2.4 10 10 10 10 10 10 10 104-WEEK Table 7 summary of clinical Hematology Data Females Week 4 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTAKE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ RBC X10'/,uL -------- HGB C/DL -------- HCT -------- mcv FL -------- MCH PG -------- MCHC PLT X101/,uL -------- -------- 0 0.5 2.0 5.0 20.0 MEAN S.D. N MEAN S.D. N ME" S.D. N MEAN S.D. N MEAN S.D. N 7.28 .276 10 7.40 .367 10 7.49 .367 10 7.40 .396 10 7.38 .238 10 1S.2 .45 10 15.3 .62 10 15.1 .70 10 15.4 .42 10 15.3 .27 10 45.1 1.20 10 45.7 1.46 10 45.2 1.64 10 45.8 1.00 10 45.6 1.07 10 62.0 1.80 10 61.8 1.36 10 60.4 1.62 10 62.0 2.25 10 61.9 1.21 10 20.8 .63 10 20.7 .58 10 20.2 .64 10 20.8 .79 10 20.8 .64 10 33.7 .41 10 33.5 .57 10 33.5 .55 10 33.6 S2 10 33.6 .53 10 1103 109.6 10 1126 181.3 10 1069 128.2 10 1104 116.6 10 1108 88.4 10 Table 7 summary of Clinical Hematology Data Females Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUORGOCTANE SULPONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ 0 0.5 MEAN S.D. N MEAN S.D. N 2.0 MEAN S.D. N 5.0 20.0 MEAN S.D. N MEAN S.D. N WBC X101/)uL -------- N-SEG X101/,uL -------- 7.4 .7 2.27 .23 10 10 6.5 .7 1.01 .78 10 10 5.0 .4 1.80 .15 10 10 69 .5 2:88 .23 10 10 6.2 .5 1 .17 10 LYMPH X103/,uL ------- MONO X10'/ML --- ---- EOSIN XIOI/,uL BASO X10'/,uL -------- -------- 6.3 2.12 10 .3 .1 .0 .20 .06 .00 10 10 10 5.5 ..313 ..005 ..000 10 1.01 10 10 10 41..833 .121 ..005 ..000 10 io 10 10 6.0 2.62 10 .2 1 .0 .11 :04 .00 10 10 10 5 4 ..124 ..014 ..000 10 1.'72 10 10 10 N-SEG% LYMPH% MONO% EOSIN% ------- --- ---- -------- -------- 9 3.5 10 84 4.0 10 5 2.2 10 2 1.4 10 10 10.8 10 9 5.1 10 8 2.1 10 84 10.4 10 84 7.9 10 87 3.7 10 4 1.7 10 5 3.5 10 4 1.7 10 1 .6 10 1 10 1 10 8 86 4 1 2.7 4.6 2.1 10 10 10 10 104-WEEK Table 8 Summary of clinical Hematology Data Males Week 14 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ RBC X10'/IjL HOB G/DL -------- -------- HCT % -------- mcv FL -------- MCH PG -------- HCHC PLT % X101/,uL -------- -------- 0 0.5 2.0 5.0 20.0 KEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N 8.55 .446 10 8.77 .441 10 8.68 .456 10 8.57 .478 10 8.23 .216 10 15.6 .69 10 is.8 .56 10 15.7 .60 10 15.2 .63 10 15.1 .51 10 45.9 1.83 10 46.7 1.45 10 46.5 1.65 10 45.2 1.61 10 44.6 1.30 10 53.7 1.52 10 53.3 1.68 10 S3.6 2.19 10 52.8 1.64 10 54.2 1.42 10 18.3 .56 10 18.0 .48 10 18.1 .60 10 17.8 .48 10 18.4 .61 10 34.1 .55 10 33.9 .53 10 33.7 .47 10 33.7 .49 10 33.9 .38 10 1142 164.6 10 1092 104.7 10 1168 189.6 10 1078 155.6 10 1139 124.3 10 104-WEEK Table 8 Summary of Clinical Hematology Data Males week 14 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ WBC X101/,uL N-SEG X101/,uL -------- -------- LYMPH XIOI/,uL -------- MONO X101/,uL -------- EOSIN X101/,uL -------- BASO X103/,uL -------- N-SEG% -------- LYMPH% -------- MONO% -------- EOSIN% -------- 0 MEAN 8.2 1.1 6.1 .8 .2 .0 14 74 10 2 S.D. 1.95 .37 1.46 .27 .07 .03 3.1 3.2. 2.1 N 10 10 10 10 10 10 10 10 10 10 0.5 MEAN 8.5 1.3 6.2 .8 .2 .0 is 73 10 2 S.D. 1.20 .28 1.04 .19 .10 .00 3.6 4.S 1.6 1. N 10 10 10 10 10 10 10 10 10 10 2.0 MEAN S.D. N 7.8 1.64 10 1.2 .33 10 5.7 1.20 10 .8 .2 .0 .29 .07 .03 10 10 10 15 73 10 2 1.9 3.8 2.8 1. 10 10 10 10 5.0 MEAN 7.8 1.2 5.7 .7 .2 .0 16 73 9 2 S.D. 2.08 .37 1.97 .20 .05 .00 4.S 6.1 2.5 N 10 10 10 10 10 10 10 10 10 10 20.0 MEAN 10.5 1.6 7.8 .9 .2 .0 16 73 8 2 S.D. 3.65 .35 3.43 .39 .08 .03 5.4 7.3 4.2 N 10 10 10 10 10 10 10 10 10 10 104-WEEK Table 8 summary of Clinical Hematology Data Females Week 14 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PRRFLUOROOCTANE SULPONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ REC X101/,uL -------- HGB G/DL -------- HCT % -------- mcv FL -------- MCH PG -------- MCHC PIT t X101/jwL -------- -------- 0 0.5 2.0 5.0 20.0 MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N 8.00 .535 10 8.22 .377 10 8.06 .253 10 7.97 .453 10 7.87 .247 10 15.3 .87 10 15.5 .59 10 15.2 .58 10 15.2 .70 10 15.1 .67 10 4S.2 2.59 10 46.0 1.80 10 45.2 1.76 10 45.0 2.17 10 44.2 1.82 10 56.6 1.18 10 56.0 1.93 10 56.1 2.37 10 S6.5 1.32 10 56.2 1.64 10 19.1 .25 10 18.9 .74 10 18.8 .72 10 19.0 .41 10 19.2 .59 10 33.8 .57 10 33.7 .91 10 33.6 .57 10 33.7 .65 10 34.1 1.08 10 1061 68.5 10 1143 67.8 10 1070 93.4 10 1082 64.7 10 1144 122.9 10 104-WEEK Table 8 Summary of clinical Hematology Data Females Week 14 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ WBC X101/,uL -------- N-SEG X101/,uL -------- LYMPH X101/,uL -------- MONO X10'/,uL -------- EOSIN X101/,uL -------- BASO X103/,uL -------- N-SEG% -------- LYMPH% -------- MONO% -------- ROSIN% -------- 0 MEAN 4.9 1.0 3.4 .4 .1 .0 20 69 8 2 S.D. 1.51 .53 1.14 .17 .07 .00 8.8 9.7 2.9 N 10 10 10 10 10 10 10 10 10 10 0.5 MEAN 6.4 1.0 4.8 .5 .1 .0 16 73 9 2 S.D. 2.64 .45 2.18 .25 .03 .00 5.1 9.1 4.0 N 10 10 10 10 10 10 10 10 10 10 2.0 MEAN 4.9 .7 3.9 .3 .1 .0 14 77 6 2 S.D. 1.65 .17 1.50 .08 .03 .00 4.4 5.1 1.2 N 10 10 10 10 10 10 10 10 10 10 5.0 MEAN 5.8 .9 4.4 .4 .1 .0 15 75 8 2 S.D. 1.96 .55 1.54 .32 .05 .03 7.1 7.7 3.9 N 10 10 10 10 10 10 10 10 10 10 20.0 MEAN 4.8 1.0 3.4 .3 .1 .0 19 72 7 2 S.D. 1.68 .64 1.12 .17 .06 .00 6.3 6.7 2.5 1 N 10 10 10 10 10 10 10 10 10 10 104-WEEK Table 9 Summary of Clinical Hematology Data Males Week 27 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ RBC X10'/ML -------- HGB G/DL -------- HCT % -------- mcv PL -------- MCH pa -------- MCHC PIT % X103/AAL -------- -------- 0 0.5 2.0 5.0 20.0 MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N 8.78 .471 10 8.84 .629 10 8.82 .301 10 9.04 .439 10 8.67 .2S2 10 15.7 .58 10 15.5 .84 10 15.5 .54 10 15.8 .60 10 15.1 .62 10 46.1 1.96 10 46.0 2.34 10 45.6 1.62 10 46.6 1.84 10 44.7 1.22 10 52.6 1.60 10 52.1 1.63 10 51.8 1.01 10 51.6 1.40 10 51.6 2.06 10 17.9 .56 10 17.6 .60 10 17.5 .45 10 17.4 .58 10 17.4 .83 10 34.0 .34 10 33.7 .31 10 33.9 .38 10 33.8 .36 10 33.8 S7 10 1235 171.4 10 1270 138.4 10 1237 127.7 10 1217 116.4 10 1287 162.9 10 104-WEEK Table 9 Summary of Clinical Hematology Data Males Week 27 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ WEC X10'/,uL -------- N-SEG X101/,uL -------- LYMPH X10'/PL -------- MONO X10'/ML EOSIN BASO X101/,uL X10'/,uL -------- -------- -------- N-SEG% LYMPH% -------- -------- MONO% EOSIN% -------- ------- 0 .2 .0 19 67 13 MEAN 7.5 1.3 5.0 1.0 S.D. 1.43 .29 1.14 .23 .08 .05 4.1 3.9 2.1 N 10 10 10 10 10 10 10 10 10 1 0.5 MEAN 8.2 1.5 5.6 .9 .2 .0 19 67 11 S.D. 1.42 .31 1.40 .19 .03 .05 5.6 6.2 2.3 N 10 10 10 10 10 10 10 10 10 1 2.0 MEAN 8.7 1.7 5.7 1.0 .2 .0 19 66 12 S.D. 1.53 .52 1.28 .26 .07 .04 6.1 7.8 2.2 N 10 10 10 10 10 10 10 10 10 1 5.0 MEAN 8.6 1.6 5.7 1.2 .2 .0 19 65 14 S.D. 1.88 .31 1.74 .32 .05 .05 4.5 6.7 3.0 N 10 10 10 10 10 10 10 10 10 1 20.0 MEAN 8.7 1.6 5.9 1.0 .1 .0 18 68 12 S.D. 1.88 .42 1.41 .28 .05 .04 3.2 4.9 2.6 N 10 10 10 10 10 10 10 10 10 1 104-WEEK Table 9 summary of clinical Hematology Data Females Week 27 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ RBC X10'/,uL -------- HGB G/DL -------- HCT % -------- mcv FL -------- MCH PG -------- MCHC PLT % X101/,uL -------- -------- 0 0.5 2.0 5.0 20.0 MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N 7.56 .563 10 7.93 .639 10 8.00 .312 10 8.12 .353 10 7.73 .398 10 14.6 .97 10 14.9 .52 10 15.1 .66 10 15.3 .61 10 14.5 .53 10 43.5 3.16 10 44.8 1.92 10 45.2 1.94 10 46.1 1.61 10 43.3 1.70 10 57.6 1.06 10 56.8 2.94 10 56.S 1.44 10 56.8 1.20 10 56.1 1.87 10 19.2 .33 10 18.9 1.18 10 18.9 S3 10 18.9 .57 10 18.8 .66 10 33.4 .63 10 33.3 .35 10 33.4 .32 10 33.3 .44 10 33.5 .39 10 1051 121.7 10 1057 127.0 10 1074 75.0 10 1095 131.4 10 1062 153.7 10 104-WEEK Table 9 sumnary of Clinical Hematology Data Females Week 27 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ WBC X101/,uL -------- N-SEG XIOI/,uL -------- LYMPH X101/juL -------- MONO X101/,uL -------- EOSIN X101/oL BASO X103/,uL -------- -------- N-SEG% -------- LYMPH% -------- MONO% -------- EOSIN% ------- 0 MEAN 5.5 .9 4.0 .6 .1 .0 16 72 10 S.D. .77 .54 .63 .26 .10 .03 9.1 9.9 3.3 N 10 10 10 10 10 10 10 10 10 1 0.5 MEAN S.D. N 6.7 4.82 10 1.1 1.22 10 5.0 3.24 10 .5 .1 .0 14 77 7 .33 .05 .03 5.3 5.6 3.2 10 10 10 10 10 10 1 2.0 5.0 20.0 MEAN S.D. N MEAN S.D. N MEAN S.D. N 4.8 1.17 10 6.1 1.95 10 5.9 1.61 10 .7 3.6 .21 .97 10 10 .8 4.7 .23 1.74 10 10 .9 4.4 ss 1.41 10 10 .4 .1 .0 15 74 a .18 .03 .00 3.1 6.0 3.4 10 10 10 10 10 10 1 .5 .1 .0 14 76 8 .24 .05 .00 4.8 6.5 3.9 10 10 10 10 10 10 1 .4 .1 .0 16 75 7 .18 .04 .00 8.4 10.7 3.2 10 10 10 10 10 10 1 104-WEEK Table 10 Summary of Clinical Hematology Data Males Week 53 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ RBC XIOI/,uL -------- HGB G/DL -------- HCT % -------- mcv FL -------- MCH PG -------- MCHC PLT % xlO3/,uL -------- -------- 0 MEAN S.D. N 9.33 .286 10 15.5 .70 10 43.5 2.02 10 52.3 1.70 10 19.6 .59 10 35.6 .38 10 1112 155.9 10 0.5 MEAN S.D. N 8.33 .266 10 15.2 .21 10 43.4 .77 10 52.1 1.23 10 18.3 .57 10 35.1 .49 10 1059 172.9 10 2.0 MEAN S.D. N 9.11 .288 10 14.9 .64 10 42.5 1.63 10 52.4 1.79 10 18.4 .75 10 35.1 .36 10 931 108.6 10 5.0 MEAN S.D. N 8.43 .536 10 15.2 .45 10 43.7 1.56 10 52.0 2.41 10 18.0 .94 10 34.7 .58 10 1011 111.9 10 20.0 MEAN S.D. N 8.28 .360 9 14.9 .71 9 41.7 1.74 9 50.4 1.58 9 17.9 .47 9 35.6 .49 9 1241 124.3 9 104-WEEK Table 10 summary of clinical Hematology Data Males Week 53 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ WBC X101/,uL N-SEG X101/,uL -------- -------- LYMPH XIOI/ML -------- MONO X101/,uL -------- EOSIN amo X10'/juL X10"/ML -------- -------- N-SEG% -------- LYMPH% -------- MONO% -------- EOSIN% -------- 0 MEAN 7.1 2.4 3.6 .9 .1 .0 34 51 13 2 S.D. 1.33 .96 .96 .26 .05 .00 11.9 10.6 3.2 N 10 10 10 10 10 10 10 10 10 10 0.5 MEAN 7.8 1.7 4.9 * 1.1 .1 .0 22 63 14 2 S.D. 1.59 .31 1.10 .32 .06 .03 3.0 4.0 2.5 N 10 10 10 10 10 10 10 10 10 10 2.0 MEAN 8.6 2.1 5.4 * 1.0 .1 .0 22 64 11 1 S.D. 2.97 1.64 1.20 .65 .04 .03 7.6 10.2 4.5 N 10 10 10 10 10 10 10 10 10 10 5.0 MEAN 8.5 2.0 5.4 * .9 .1 .0 24 63 11 2 S.D. 1.63 .74 1.28 .33 .04 .00 6.2 7.9 2.6 N 10 10 10 10 10 10 10 10 10 10 20.0 MEAN S.D. N 7.3 1.78 9 2.3 1.sg 9 4.0 1.29 9 .8 .1 .0 30 56 12 2 .42 .03 .03 12.3 15.1 5.0 9 9 9 9 9 9 9 104-WEEK Table 10 Summary of Clinical Hematology Data Females Week 53 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT (PPOS, T-6295) IN RATS DOSE ppm T-6295 ------ RBC X101/,uL -------- HGB HCT C/DL % -------- -------- mcv MCH FL PG -------- -------- MCHC PLT t X101/mL -------- -------- 0 0.5 2.0 5.0 20.0 MEAN S.D. N MEAN S.D. N MEAN S.D. N HEM S.D. N MEAN S.D. N 7.39 .485 10 7.26 .695 10 7.31 .566 10 7.32 .479 10 7.05 .881 10 14.5 .76 10 14.2 1.04 10 14.3 .61 10 14.2 .56 10 13.5 1.66 10 40.5 1.84 10 41.0 3.11 10 41.1 1.91 10 41.1 1.73 10 38.1 4.56 10 54.9 1.64 10 56.6 2.40 10 56.3 2.32 10 S6.2 1.96 10 54.0 1.25 10 19.6 .60 10 19.7 .94 10 19.7 .89 10 19.5 .83 10 19.2 .46 10 35.7 .31 10 34.7 * .42 10 34.9 * .45 10 34.7 * .40 10 35.5 .29 10 1009 80.9 10 908 99.0 10 851 101.9 10 837 $5.9 10 1023 113.7 10 104-WEEK Summary Table 10 of Clinical Hematology Data Females Week 53 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT (PPOS; T-6295) IN RATS DOSE ppm T-6295 ------ WBC X10'/,uL -------- N-SEG X101/,uL -------- LYMPH X101/,uL -------- MONO X10"/j4L -------- EOSIN BASO X101/,uL X10'/,uL -------- -------- N-SEG% -------- LYMPH% -------- MONO% -------- EOSIN% -------- 0 MEAN 4.2 1.6 1.9 .5 .1 .0 37 49 12 2 S.D. 1.72 1.16 .57 .16 .00 .00 10.5 8.8 3.2 N 10 10 10 10 10 10 10 10 10 10 0.5 MEAN 4.7 1.0 3.1 * .5 .1 .0 22 64 12 2 S.D. 1.53 .28 1.27 .20 .03 .00 4.7 7.2 4.6 N 10 10 10 10 10 10 10 10 10 10 2.0 MEAN 5.2 1.5 3.1 * .5 .1 .0 28 59 10 3 S.D. 1.28 .54 .94 .16 .05 .00 7.4 8.6 2.5 N 10 10 10 10 10 10 10 10 10 10 5.0 MEAN 4.2 1.2 2.5 .5 .1 .0 27 60 11 2 S.D. 1.27 .58 .70 .19 .04 .00 8.2 8.2 3.9 N 10 10 10 10 10 10 10 10 10 10 20.0 MEAN 5.4 2.1 2.6 .5 .1 .0 34 53 10 2 S.D. 2.33 1.94 .90 .27 .04 .00 17.1 17.6 2.5 1 N 10 10 10 10 10 10 10 10 10 10 104-WEEK Table 11 Summary of Clinical Chemistry Data Males Week 4 DIETARY CHROMIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ GLU MG/DL -------- UN MG/DL -------- CREAT MG/DL -------- T PRO C/DL -------- ALE G/DL -------- GLOB O/DL T BILI MG/DL -------- -------- CHOL MG/DL -------- 0 o.5 2.0 5.0 20.0 MEAN S.D. m MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N 97 12 .6 6.2 4.4 1.8 .1 49 11.4 1.2 .18 .43 .26 .32 .09 6.6 10 10 10 10 10 10 10 10 97 12 .6 6.4 4.6 1.8 .1 49 5.2 1.8 .06 .33 .29 .31 .09 6.5 10 10 10 10 10 id 10 10 91 12 .6 6.0 4.5 1.6 .1 44 10.8 1.3 .14 .33 .29 .16 .08 8.6 10 10 10 10 10 10 10 10 94 12 .6 6.3 4.6 1.7 .0 48 8.9 2.1 .10 .29 .19 .15 .05 15.3 10 10 10 10 10 10 10 10 84 13 .6 6.3 4.6 1.7 .1 45 5.0 1.5 .12 .23 .18 .13 .09 13.7 10 10 10 10 10 10 10 10 104-WM Table 11 Summary of Clinical chemistry Data Males Week 4 DIETARY CHRONIC ToxicITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANS SULPONIC ACID POTASSIUM SALT (PPOS; T-6295) IN RATS DOSE ppm T-6295 ------ AST/SGOT IU/L -------- ALT/SCPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- 0 0.5 2.0 5.0 20.0 MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N 122 26.4 10 146 28.9 10 104 22.6 10 114 17.2 10 131 19.9 10 31 3.4 10 32 4.2 10 28 5.2 10 30 3.8 10 33 3.0 10 1 1.3 10 0 .7 10 1 1.6 10 0 .7 10 1 1.3 10 10.7 .41 10 10.7 .31 10 10.6 .41 10 10.9 .28 10 10.6 .31 10 10.0 .54 10 9.5 .62 10 10.1 .39 10 9.8 .62 10 9.8 .59 10 148 1.6 10 147 1.3 10 148 1.4 10 147 1.4 10 148 1.4 10 6.3 .69 10 6.3 .42 10 6.2 .71 10 6.2 .62 10 6.1 .58 10 104 1.5 10 104 1.1 10 105 1.1 10 103 2.2 10 103 .9 10 104-WEEK Table 11 summary of Clinical chemistry Data Females Week 4 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SAIT (PFOS; T-6295) IN PATS DOSE ppm T-6295 ------ GLU MG/DL UN MG/DL CREAT MG/DL -------- -------- -------- T PRO G/DL ALB O/DL GLOB G/DL -------- -------- -------- T BILI MG/DL CHOL MG/I)L -------- -------- 0 MEAN 114 14 .7 6.9 5.1 1.8 .1 72 S.D. 7.1 1.7 .10 .14 .11 .16 .10 11.5 N 10 10 10 10 10 10 10 10 0.5 MEAN ill 13 .8 6.8 5.0 1.8 .0 70 S.D. 7.0 1.8 .09 .16 .18 .23 .07 25.8 N 10 10 10 10 10 10 10 10 2.0 MEAN 113 14 .8 6.8 5.1 1.7 .0 61 S.D. 17.9 1.8 .13 .39 .32 .19 .07 17.7 N 10 10 10 10 10 10 10 10 5.0 MEAN 109 13 .7 7.1 5.3 1.8 .0 S8 S.D. 11.0 1.2 .08 .35 .31 .17 .04 14.8 N 10 10 10 10 10 10 10 10 20.0 MEAN 107 14 .7 6.8 5.2 1.6 .0 54 S.D. 7.7 1.8 .10 .3S .19 .18 .10 10.7 N 10 10 10 10 10 10 10 10 104-WEEK Table 11 Sumary of Clinical Chemistry Data Females Week 4 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ AST/SCOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA KHOL/L K KNOL/L CL MMOL/L -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN S.D. N 123 28.2 10 32 4.8 10 0 .7 10 10.8 .34 10 8.5 .90 10 145 1.0 10 5.9 .43 10 104 1.3 10 2.0 5.0 20.0 MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N 120 37.2 10 101 12.2 10 112 23.9 10 92 16.0 10 31 5.6 10 29 5.6 10 32 5.1 10 32 7.1 10 1 1.4 10 2 2.0 10 2 1.3 10 1 1.1 10 10.6 .31 10 10.7 .37 10 10.7 .35 10 10.6 .24 10 8.4 1.03 10 8.5 1.09 10 7.9 .83 10 8.2 1.47 10 145 .8 10 147 1.3 10 147 2.2 10 146 1.8 10 6.1 .45 10 6.2 .51 10 6.1 .41 10 6.0 .56 10 104 9 10 106 1.2 10 105 1.5 10 105 1.1 10 Table 11 Summary of Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CHROMIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 PCOAO IU/G ------ -------- 0 MEAN 9 S.D. 2.2 N 5 0.5 MEAN 9 S.D. 2.3 N 5 2.0 MEAN 7 S.D. 4.0 N 5 5.0 MEAN a S.D. .8 N 5 20.0 MEAN 6 S.D. 1.4 N 5 104-WEEK Table 11 Summary of Clinical Chemistry Data Females week 4 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ PCOAO IU/C -------- 0 MEAN 5 S.D. 1.5 N 5 O.S MEAN 6 S.D. 1.1 N 5 2.0 MEAN 3 S.D. 1.7 N 5 5.0 MEAN 2 S.D. 1.1 N 5 20.0 MEAN 4 S.D. 1.1 N 5 104-WEEK Table 12 Summary of clinical Chemistry Data Males Week 14 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PMWLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE PPM T-6295 GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL -------- -------- -------- -------- -------- -------- -------- -------- 0 MEAN 102 13 .4 6.9 4.9 2.0 .30 .2 63 .18 13.4 S.D. 6.5 1.8 .14 .41 .26 10 10 10 10 10 10 10 N 10 o.5 MEAN 106 14 .6 7.0 5..020 2..026 ..215 5136.6 S.D. 11.2 101.6 .1014 1.034 10 10 10 10 N 10 2.0 MEAN S.D. N 91 13 .5 6.7 4.7 2..025 ..112 5114.5 13.7 2.0 .10 .34 .16 10 10 10 10 10 10 10 10 5.0 MEAN 99 14 .6 7.0 4.9 2.1 .2 57 6.9 8.9 1.4 .12 10 .46 10 .29 10 .20 10 .15 10 10 S.D. N 10 10 20.0 MEAN 95 16 .6 6.9 4.9 2.0 .1 37 12.9 1.6 .16 .34 .24 .18 .13 10 10 S.D. N 10.3 10 10 10 10 10 10 104-WEEK Table 12 Summary of Clinical Chemistry Data Males Week 14 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID P6TASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 AST/SGOT IU/L ALT/SGPT IU/L -------- -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MMOL/L -------- 0 0.5 2.0 5.0 20.0 MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N ill 23.1 10 112 18.8 10 127 20.5 10 129 1S.4 10 128 60.7 10 36 7.0 10 41 6.3 10 41 4.7 10 44 13.6 10 65 52.9 10 1 1.5 10 1 .7 10 1 1.3 10 1 1.6 10 1 1.6 10 10.5 .28 10 10.5 .29 10 10.4 .20 10 10.5 .31 10 10.5 .23 10 7.5 .57 10 7.8 .64 10 7.8 .64 10 7.5 .57 10 7.8 .58 10 146 .7 10 147 2.6 10 147 1.8 10 147 2.8 10 146 1.3 10 6.2 .37 10 6.5 .32 10 6.4 .46 10 6.1 .37 10 6.4 .52 10 105 1.2 10 105 2.0 10 105 2.2 10 104 2.0 10 104 1.4 10 104-WEEK Table i2 Summary of Clinical chemistry Data Females Week 14 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTAKE SULPONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ GLU MG/DL UN MG/DL CREAT MG/DL -------- -------- -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- 0 MEAN 106 15 .6 7.6 5.6 2.1 .1 75 S.D. 12.0 1.7 .12 .34 .25 .22 .19 14.9 N 10 10 10 10 10 10 10 10 0.5 MEAN 106 15 .6 8.2 6.2 2.0 .13 .42 .44 .19 .2 88 .14 27.1 S.D. N 9.1 3.2 10 10 10 10 10 10 10 10 2.0 MEAN 108 is .8 7.9 5.9 2.0 .1 87 S.D. 5.9 2.3 .12 .30 .24 .22 .08 23.9 N 10 10 10 10 10 10 10 10 5.0 MEAN S.D. N 95 15 .6 8.0 5.9 2.1 8.3 2.0 .14 .44 .43 .31 10 10 10 10 10 10 .2 70 .14 13.4 10 10 20.0 MEAN S.D. N 99 18 .6 8.0 6.1 1.9 .2 66 6.7 2.5 .05 .45 .33 .19 .14 14.0 10 10 10 10 10 10 10 10 S6Z9-,L ME EBT-6ZE9 SC)U'?AOD 01 O*E 90T OT LS' Z'9 OT 9*Z OtT OT LL" S'9 OT OE' B*OT OT S*T T OT Z'si 6E OT L*Ot 06 -a-s mvm 0*0 OT 6*T LOT OT V*T SOT 01 ZL* B*s OT TL* Z*9 OT O'Z ati OT O'E OPT OT till 9'9 OT LB* S'9 OT TS' L*OT OT Et' 6'OT OT 6*T z ot l,'T T OT L't 1,E OT 6*Ll LE OT S'9T EOT OT 6*9Z EOT u a.s mvm 0' u U.s tmam 0* OT 9*T 90T OT 9L* V'9 01 T*Z SVT OT OE'T V"9 OT 6v* T*Tl OT L*l T ol T'6 9E OT O'LT LOT u -a-s mvzx S' OT 6' LOT OT Et' 0*9 OT O'Z OPT OT S6, E*9 OT st* LIOT OT Z'l T OT V'Z VE 01 E*9T EOT -a-s HYM -------- -------- -------- -------- ------- 1/170mm Ila I/IIOKK I/rIONK yu ria/f)m SOHD I ria/f)m yo -------- ------- -------- I/iii ILE)E) rilni ri/nI S6Z9-,L ul xdos/,Lrm JODS/JSV as s,LvliH, (S6Z9-.L !sojd) Lrms KnIssyLLod ajoy aimodirins MN.LDW'dofiriaHad H.Ljt4)LA(IJSA.LIDIN3E)OHI3HVD aNY ALIDIXO.L OINODHZ) AUVJZIA PT '5(set4 SeTieulea t-qua Ai-4sTuielqoivoTuTTo ;o Aieumins ZI elqw.L XHRM-poT 104-WEEK Table 12 summary of Clinical Chemistry Data Males week 14 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ PCOAO IU/G -------- 0 MEAN 5 S.D. 1.3 N 5 0.5 MEAN 5 S.D. 3.3 N 5 2.0 MEAN 5 S.D. 3.0 N 5 5.0 MEAN 2 S.D. 1.8 N 5 20.0 MEAN 5 S.D. 1.9 N 5 104-WEEK Table 12 Summary of Clinical Chemistry Data Females Week 14 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ PCOAO IU/G -------- 0 MEAN 2 S.D. 1.6 N 5 0.5 MEAN 3 S.D. 2.4 N 5 2.0 MEAN 1 S.D. .7 N 5 5.0 MEAN 2 S.D. 2.6 N 5 20.0 MEAN 5 S.D. 2.9 N 5 104-WEEK Table 13 summary of clinical Chemistry Data Males Week 27 DIETARY CHRONIC ToxiciTy AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT (PPOS; T-6295) IN RATS DOSE ppm T-6295 ------ CLU MG/DL ---- --- UN MG/DL CRRAT MG/DL T PRO C/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL -------- -------- -------- -------- -------- -------- -------- 0 MEAN 115 12 .7 7.3 4.9 2.4 .2 89 S.D. N 8.1 2.3 .10 .43 10 10 10 10 .28 .34 10 10 .12 24.7 10 10 O.S MEAN 117 13 .8 7.1 4.7 2.4 .3 84 S.D. 14.7 1.7 .16 .48 .30 .33 .06 31.8 N 10 10 10 10 10 10 10 10 2.0 MEAN 114 13 .8 7.2 4.8 2.4 .4 74 S.D. 13.2 2.3 .08 .29 .23 .26 .16 1S.5 N 10 10 10 10 10 10 10 10 5.0 MEAN 113 14 .7 7.4 4.9 2.5 .3 72 S.D. 11.7 2.5 .16 .45 .28 .24 .12 28.2 N 10 10 10 10 10 10 10 10 20.0 MEAN ill 17 .8 7.5 5.1 2.4 .34 .25 .19 .2 46 .10 11.6 S.D. N 12.8 2.1 .15 10 10 10 10 10 10 10 10 104-WEEK Table 13 Summary of clinical Chemistry Data Males Week 27 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTAKE SULPONIC ACID POTASSIUM SALT (PPOS; T-6295) IN RATS DOSE ppm T-6295 AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL NHOL/L -------- 0 0.5 2.0 5.0 20.0 MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N 84 17.8 10 81 13.0 10 92 22.0 10 80 9.7 10 103 53.6 10 37 6.2 10 36 6.1 10 47 10.5 10 38 6.3 10 65 48.7 10 1 1.3 10 2 1.8 10 1 .6 10 1 1.4 10 1 1.9 10 11.1 .36 10 11.1 .43 10 11.1 .28 10 11.3 .57 10 11.2 .34 10 6.7 .36 10 6.9 .59 10 6.8 .30 10 7.1 .62 10 7.3 .92 10 146 1.1 10 147 1.8 10 147 1.9 10 148 1.9 10 147 2.5 10 6.7 .23 10 7.0 .53 10 6.7 S4 10 6.9 .46 10 6.9 .65' 10 104 2.0 10 104 1.4 10 104 i.s 10 104 1.4 10 104 1.4 10 104-WEEK Table 13 Sumary of Clinical Chemistry Data Females Week 27 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ GLU MG/DL -------- UN MG/DL CREAT MG/DL -------- -------- T PRO G/DL ALB G/DL GLOB G/DL -------- -------- -------- T BILI MG/DL -------- CHOL MG/DL -------- 0 0.5 2.0 5.0 20.0 MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N 104 7.3 10 98 6.3 10 99 8.2 10 98 10.0 10 94 6.8 10 14 1.3 10 15 2.1 10 15 1.9 10 15 3.2 10 20 2.8 10 .9 .17 10 .9 .21 10 1.0 .16 10 .9 .14 10 .9 .13 10 8.9 .37 10 8.4 .66 10 8.4 .71 10 8.3 .39 10 8.7 .55 10 6.4 .41 10 6.2 s9 10 6.0 .65 10 5.9 .28 10 6.4 .37 10 2.5 .20 10 2.3 .29 10 2.4 .26 10 2.3 .29 10 2.3 .31 10 .4 115 .14 26.0 10 10 .3 95 .10 29.0 10 10 .3 78 .13 16.3 10 10 .3 79 .15 10.9 10 10 .4 80 .14 24.4 10 10 104-WEEK Table 13 summary of clinical chemistry Data Females Week 27 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ AST/SGOT IU/L -------- ALT/SGPT IU/L -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL ---- --- NA MMOL/L K MMOL/L CL MMOL/L -------- -------- -------- 0 0.5 2.0 5.0 20.0 MEAN S.D. N MEAN S.D. N MFAN S.D. N MEAN S.D. N MEAN S.D. N 119 71.9 10 102 18.6 10 143 116.7 10 117 39.6 10 117 62.6 10 64 49.4 10 34 8.4 10 77 130.6 10 47 25.8 10 64 55.5 10 2 2.0 10 2 1.5 10 2 2.0 10 4 1.7 10 2 2.6 10 11.7 .34 10 11.7 .67 10 11.2 .38 10 11.2 .37 10 11.5 .44 10 6.7 .77 10 6.5 .74 10 6.4 .96 10 6.7 .75 10 7.3 1.20 10 148 2.1 10 147 2.2 10 148 2.4 10 148 1.9 10 148 2.9 10 5.8 .71 10 5.9 .69 10 5.9 .57 10 6.0 .63 10 6.0 .67 10 106 2.3 10 106 1.9 10 107 1.8 10 108 2.0 10 106 2.0 10 104-WEEK Table 14 summary of clinical Chemistry Data Males Week 53 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE PPM T-6295 AST/SGOT IU/L ALT/SGPT IU/L GGT IU/L -------- -------- -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K MMOL/L -------- CL MKOL/L -------- 0 o.5 2.0 5.0 20.0 MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N 106 27.0 10 118 38.8 10 ill 16.6 10 130 21.9 10 129 70.0 9 54 65.8 10 62 51.8 10 40 7.5 10 44 8.3 10 83 84.4 9 0 .0 10 0 .5 10 0 .8 10 0 1.3 10 1 1.3 9 10.5 .39 10 11.4 * .41 10 11.0 * .43 10 11.1 .55 10 10.5 .41 9 6.3 .42 10 7.1 * .34 10 7.2 * .59 10 7.7 .64 10 7.0 .61 9 147 1.6 10 148 2.5 10 149 2.4 10 149 3.5 10 148 3.5 9 5.9 .44 10 6.5 .55 10 6.6 .56 10 6.8 .63 10 6.2 .89 9 106 1.8 10 106 1.9 10 107 1.7 10 107 2.4 10 110 2.1 9 104-WEEK Table 14 Summary of Clinical Chemistry Data Females Week 53 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ AST/SGOT IU/L ALT/SGPT IU/L -------- -------- GGT IU/L -------- CA MG/DL -------- I PHOS MG/DL -------- NA MMOL/L -------- K KNOL/L -------- CL MMOL/L -------- 0 O.S 2.0 5.0 20.0 MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N 119 61.0 10 115 15.3 10 121 21.4 10 108 2S.1 10 93 17.5 10 50 30.2 10 43 7.1 10 54 14.5 10 46 19.5 10 46 17.3 10 0 .3 10 0 .5 10 1 1.3 10 1 1.7 10 0 .3 10 10.6 .54 10 11.1 .48 10 10.8 .24 10 11.0 .29 10 10.6 .47 10 5.8 .90 10 5.8 .83 10 5.6 .58 10 5.5 .74 10 5.9 .65 10 146 2.4 10 147 3.6 10 147 2.1 10 147 1.8 10 147 3.1 10 5.1 .47 10 5.8 1.04 10 5.1 .45 10 5.4 .65 10 S.3 1.00 10 106 2.6 10 106 3.7 10 107 2.5 10 108 3.2 10 110 2.7 10 104-WEEK Table 14 Summary of Clinical Chemistry Data males Week 53 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PPOS; T-6295) IN RATS DOSE ppm T-6295 ------ GLU MG/DL -------- UN NO/DL -------- CREAT MG/DL -------- T PRO G/DL ALB G/DL -------- -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- 0 MEAN S.D. N 129 13.2 10 10 1.4 10 .6 7.0 4.4 2.6 .17 .46 .35 .33 10 10 10 10 0.5 MEAN S.D. N 125 12.5 10 12 1.2 10 .7 7.4 4.8 .09 .26 .26 10 10 10 2.6 .28 10 2.0 MEAN S.D. N 121 13.8 10 12 1.5 10 .7 7.3 .15 .38 10 10 4.7 .20 10 2.6 .32 10 5.0 MEAN S.D. N 121 8.0 10 14 1.3 10 .7 7.6 .16 .46 10 10 4.8 .28 10 2.7 .32 10 20.0 MEAN S.D. N 110 10.2 9 13 2.3 9 .7 7.0 4.6 .09 .61 .5S 9 9 9 2.4 .23 9 .0 120 .07 44.6 10 10 .2 129 .16 53.1 10 10 .1 89 .09 17.7 10 10 .1 92 .12 15.2 10 10 .0 50 .03 17.8 9 9 104-WEEK Table 14 Summary of Clinical chemistry Data Females Week 53 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ GLU MG/DL -------- UN MG/DL CREAT MG/DL -------- -------- T PRO G/DL -------- ALB G/DL -------- GLOB G/DL -------- T BILI MG/DL -------- CHOL MG/DL -------- 0 MEAN S.D. N ill 10.1 10 11 2.5 10 .7 7.7 .10 1.04 10 10 5.5 .81 10 2.2 .38 10 0.5 MEAN S.D. N 102 8.4 10 13 2.3 10 .7 8.1 5.8 2.3 .25 .44 .34 .19 10 10 10 10 2.0 MEAN S.D. N 96 12.9 10 13 2.2 10 .8 8.0 S.S 2.5 .15 .45 .44 .46 10 10 10 10 5.0 MEAN S.D. N 97 14 .8 8.2 5.8 2.4 6.3 2.9 .19 .31 .32 .30 10 10 10 10 10 10 20.0 MEAN S.D. N 98 9.8 10 16 2.8 10 .8 7.9 .14 .76 10 10 5.7 .81 10 2.2 .39 10 .0 116 .07 19.8 10 10 .2 108 .14 26.1 10 10 .1 101 .11 17.2 10 10 .1 110 .13 49.6 10 10 .0 79 .10 25.5 10 10 Table 15 Summary of clinical Urinalysis Data males Week 4 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PPOS; T-6295) IN RATS DOSE ppm T-6295 ------ U VOL ML SP GR U PH -------- -------- -------- 0 MEAN S.D. N 21.4 11.72 10 1.010 .0036 10 7.0 .16 10 2.0 5.0 20.0 MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N 22.6 14.76 10 25.3 16.35 10 31.8 14.52 10 22.6 9.78 10 1.014 .0080 10 1.010 .0073 10 1.009 .0055 10 1.012 .0069 10 7.0 .24 10 7.0 .33 10 7.2 .24 10 7.0 .00 10 104-WEEK Table 15 Summary of Clinical Urinalysis Data Females Week 4 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTAM SULPONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ U VOL ML SP GR U PH -------- - ------ -------- 0 0.5 2.0 5.0 20.0 MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N 10.9 13.48 10 15.2 16.13 10 14.2 17.68 10 13.2 7.12 10 17.5 16.39 10 1.019 .0103 10 1.015 .0106 10 1.015 .0087 10 1.014 .0060 10 1.013 .0083 10 6.8 .26 10 6.8 .24 10 6.8 .26 10 6.8 .24 10 6.8 .26 10 104-WEEK Table 16 summary of Clinical Urinalysis Data Males Week 14 DIETARY CHROMIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ U VOL ML SP GR -------- -------- U PH -------- 0 0.5 2.0 5.0 20.0 MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N 28.8 16.45 10 23.5 16.23 10 20.8 17.37 10 25.4 14.27 10 18.5 14.64 10 1.014 .0099 10 1.018 .0140 10 1.018 .0099 10 1.014 .0067 10 1.019 .0073 10 7.2 .34 10 7.1 .39 10 7.0 .31 10 7.0 .33 10 7.0 .28 10 104-WEEK Table 16 Summary of Clinical Urinalysis Data Females Week 14 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFIUOROGCTANE SUTFONIC ACID POTASSIUM SALT (PPOS; T-6295) IN RATS DOSE ppm T-6295 ------ u voij ML. SP GR U PH -------- -------- -------- 0 MFAN S.D. N 0.5 MEAN S.D. N 2.0 MEAN S.D. N 5.0 MEAN S.D. N 20.0 MEAN S.D. N 17.5 21.04 10 16.8 17.86 10 7.8 6.43 10 25.7 14.20 10 19.5 17.83 10 1.020 .0166 10 1.016 .0090 10 1.021 .0082 10 1.009 .0050 10 1.015 .0119 10 6.8 .26 10 7.o .50 10 6.8 .35 10 6.8 .35 10 6.9 .32 10 104-WEEK Table 17 Summary of Clinical Urinalysis Data Males Week 27 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PPOS; T-6295) IN RATS DOSE ppm T-6295 ------ U VOL ML -------- SP OR -------- U PH -------- 0 MEAN S.D. N 15.2 7.12 10 1.022 .0105 10 6.9 .32 10 o.5 MEAN S.D. N 16.2 8.42 10 1.017 .0056 10 7.1 .21 10 2.0 MEAN S.D. N 16.2 6.79 10 1.019 .0053 10 7.0 .37 10 5.0 MEAN S.D. N 20.4 15.70 10 1.019 .0107 10 7.0 .24 10 2060 MEAN S.D. N 17.9 16.42 10 1.029 .0231 10 6.8 .26 10 104-WEEK Table 17 Summary of Clinical Urinalysis Data Females Week 27 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ U VOL ML -------- SP GR U PH -------- -------- 0 0.5 2.0 5.0 20.0 MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N 17.6 17.05 10 15.9 12.34 10 15.6 12.72 10 21.6 14.18 10 13.7 11.58 10 1.016 .0142 10 1.016 .0089 10 1.016 .0092 10 1.014 .0104 10 1.022 .0170 10 6.6 .21 10 6.6 .34 10 6.8 .24 10 6.9 .32 10 6.6 .37 10 104-WEEK Table 18 Sumary of clinical Urinalysis Data Males Week 53 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PPOS; T-6295) IN RATS DOSE ppm T-6295 ------ U VOL ML -------- SP GR U PH -------- -------- 0 MEAN 12.5 1.025 S.D. 7.75 .0110 N 10 10 6.6 .32 10 0.5 MEAN S.D. N 18.0 13.20 10 1.026 .0123 10 6.7 .26 10 2.0 MEAN S.D. N 16.2 7.90 10 1.021 .0063 10 7.1 .42 9 5.0 MEAN S.D. N 11.6 7.S3 10 1.032 .0192 10 6.9 .39 10 20.0 MEAN S.D. N 11.0 4.55 9 1.029 .0074 9 6.9 .49 9 104-WEEK Table 18 summary of Clinical Urinalysis Data Females Week 53 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROO@TANE SULPONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ U VOL ML -------- SP CR -------- U PH -------- 0 0.5 2.0 S.0 20.0 MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N 9.1 2.00 10 7.7 4.07 10 9.0 5.46 10 8.3 12.54 10 10.1 13.35 10 1.022 .0039 10 1.025 .0070 10 1.025 .0114 10 1.030 .0105 10 1.028 .0117 10 6.5 .33 10 6.6 .52 10 6.5 .33 10 6.6 .41 10 6.2 .26 10 104-WEEK Table 18 Sumary of Clinical Urinalysis Data Males Week 53 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ U VOL ML -------- SP GR -------- U PH -------- 0 MEAN S.D. N O.S MEAN S.D. N 12.5 7.75 10 18.0 13.20 10 1.025 .0110 10 1.026 .0123 10 6.6 .32 10 6.7 .26 10 2.0 MEAN S.D. N 5.0 MEAN S.D. N 16.2 7.90 10 11.6 7.53 10 1.021 .0063 10 1.032 .0182 10 7.1 .42 9 6.9 .39 10 20.0 MEAN S.D. N 11.0 4.55 9 1.028 .0074 9 6.9 .49 9 104-WEEK Table 18 Summary of Clinical Urinalysis Data Femles Week 53 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS: T-6295) IN RATS DOSE ppm T-6295 ------ U VOL ML SP GR -------- -------- U PH -------- 0 MEAN S.D. N 0.5 MEAN S.D. N 9.1 2.00 10 7.7 4.07 10 1.022 .0039 10 1.025 .0070 10 6.5 .33 10 6.6 .52 10 2.0 5.0 20.0 MEAN S.D. N MEAN S.D. N MEAN S.D. N 9.0 5.46 10 8.3 12.54 10 10.1 13.35 10 1.025 .0114 10 1.030 .0105 10 1.028 .0117 10 6.5 .33 10 6.6 .41 10 6.2 .26 10 104-WEEK Table 19 summary of urine chemistry Data Males Week 4 DIETARY CHRONIC TOXICITY AND CARCINC>GENICITY STUDY WITH PERFLUORO(>CTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC KNOL -------- 0 0.5 2.0 5-0 20.0 MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N 12 3.5 10 19 13.9 10 13 9.5 10 14 7.9 10 17 16.4 10 65 20.2 10 86 53.4 10 65 42@S 10 54 32.0 10 68 39.1 10 .22 .092 9 .29 .096 9 .24 .114 7 .40 .186 7 .28 .086 10 1.18 .258 9 1.35 .191 9 1.18 .211 7 1.47 .289 7 1.23 .175 10 104-WEEK Table 19 summary of urine chemistry Data Females Week 4 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- 0 0.5 2.0 5.0 20.0 MEAN S.D. N MEAN S.D. N MEAN S.D. N HEM S.D. N MEAN S.D. N 22 10.6 10 23 18.0 10 28 16.7 10 22 12.2 10 20 12.3 10 103 59.9 10 86 74.1 10 80 43.9 10 70 34.1 10 67 40.2 10 .13 .049 9 is .071 8 .20 .069 8 .24 .076 10 .18 .048 8 .56 .198 9 .59 .152 8 .57 .198 8 .75 .222 10 .66 .130 8 104-WEEK Table 20 summary of Urine Chemistry Females Week 14 Data DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUH SALT (PPOS; T-6295) IN RATS DOSE ppm T-6295 ------ U NA MMOL/L UK MMOL/L -------- -------- NA EXC MMOL -------- K EXC MMOL -------- 0 0.5 2.0 5.0 20.0 MEAN S.D. N MEAN S.D. N HEAN S.D. N MEAN S.D. N MEAN S.D. N 32 23.2 9 27 17.6 10 43 21.2 10 16 9.1 10 37 40.5 10 77 53.0 9 70 43.6 10 106 44.1 10 42 25.2 10 69 68.9 10 .22 .058 6 .20 .06S 8 .26 .146 10 .31 .085 8 .35 .197 8 .54 .172 6 .56 .212 8 .63 .234 10 .88 .281 8 .65 .259 8 104-WEEK Table 20 Summary of Urine Chemistry Data males Week 14 DIETARY CHRONIC TOXICITY AND CARCINOGMCITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ U NA MMOL/L UK MHOL/L NA EXC KNOL K EXC MMOL -------- -------- -------- -------- 0 0.5 2.0 5.0 20.0 MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N 20 17.1 10 25 16.5 10 23 12.5 10 22 11.7 10 26 13.2 10 60 50.1 10 87 80.7 10 82 56.0 10 65 34.8 10 89 37.8 10 .43 .255 7 .38 .165 8 .33 .180 8 .44 .104 8 .37 .182 10 1.17 .294 7 1.13 .314 8 1.01 .372 8 1.33 .226 8 1.24 .438 10 104-WEEK Table 21 Summary of Urine Chemistry Data Females Week 27 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ U NA MMOL/L uK MMOL/L -------- -------- NA EXC KNOL K EXC MMOL -------- -------- 0 0.5 2.0 5.0 20.0 MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N 18 18.0 10 14 9.9 10 21 18.1 10 18 17.2 10 32 37.4 10 71 68.2 10 74 50.2 10 70 49.9 10 67 56.7 10 96 85.7 10 .14 .070 8 .16 .055 9 .19 .083 10 .22 .139 9 .21 .119 10 .58 .241 8 .79 .212 9 .66 .158 10 .84 .280 9 .73 .270 10 104-WEEK Table 21 Summary of Urine Chemistry Data Males Week 27 DIETARY CHRONIC TOXICI7rY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- 0 MEAN S.D. N 22 14.5 10 116 67.1 10 .29 .153 10 1.43 .422 10 0.5 MEAN S.D. N 16 10.0 10 77 28.1 10 .22 .098 10 1.10 .234 10 2.0 MEAN S.D. N 24 9.9 9 93 25.3 9 .35 .192 9 1.27 .333 9 5.0 MEAN S.D. N 21 15.5 10 89 65.7 10 .30 .122 8 1.22 .394 8 20.0 MEAN S.D. N 46 42.4 10 139 114.S 10 .41 .226 8 1.31 .532 8 104-WEEK Table 22 Summary of Urine Chemistry Data males Week 53 DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-6295 ------ U NA MMOL/L -------- uK MMOL/L -------- NA EXC MMOL -------- K EXC MMOL -------- 0 MEAN S.D. N 32 15.5 10 133 65.6 10 .34 .141 10 1.30 .390 10 0.5 MEAN S.D. N 2.0 MEAN S.D. N 23 18.2 10 21 11.4 10 144 103.4 10 96 30.6 10 .27 .158 9 .31 .168 10 1.65 .354 9 1.40 .401 10 5.0 MEAN S.D. N 32 21.5 10 168 105.3 10 .27 .115 10 1.41 .228 10 20.0 MEAN S.D. .N 46 20.0 9 145 36.4 9 .49 .215 9 1.51 .473 9 104-WEEK Table 22 summary of Urine Chemistry Data Females Week 53 DIETARY CHRONIC TOXICITY AND CARCINOCENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295) IN RATS DOSE ppm T-629S ------ U NA MMOL/L -------- UK MMOL/L -------- NA EXC MMOL -------- K F.XC KNOL -------- 0 o.5 2.0 S.0 20.0 MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N 40 14.8 10 29 13.6 10 23 8.8 10 37 14.5 10 42 22.7 10 135 42.4 10 132 SI.3 10 140 69.9 10 172 80.9 10 164 85.4 10 .36 .122 10 .22 .163 10 .18 .081 10 .21 .147 10 .26 .196 9 1.19 .337 10 .87 .278 10 .99 .307 10 .76 .239 10 .93 .226 9 TABLE 23 Summary of Organ weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LF ADRENAL -- --- ---- -- ----- -- --- ---- --- ---- -- --- --- ---- --- --- ---- - TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGP SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BOD GROUP (g) (g). (%) RATIO GROUP (g) (g) ------------------------------------------------------------------------------------------------------------- m I p -- ---- -- ----- -- ---- - -- --- ---- -- ---- ---- -- --- ---- --- - NUMBER IN GROUP: 5 5 5 5 NUMBER IN GROUP: 5 5 5 MEAN: 495.5 0.0331 0.0068 0.0151 MEAN: 284.0 0.0443 0. STANDARD DEV: 55.9 0.0016 0.0010 0.0011 STANDARD DEV: 39.2 0.0073 0. ------------------------------------------------------------------------------------------------------------- m 2 ---- --- --- ---- --- ---- -- ----- --- F. . .2 . . . . . . . . . . . . . . . . . . . . NUMBER IN GROUP: MEAN: STANDARD DEV: 5 5 5 5 NUMBER IN GROUP: 5 5 5 481.0 0.0296 0.0061 0.0133 MEAN: 298.9 0.0346 0. 51.3 0.0071 0.0011 0.0032 STANDARD DLIV: 40.8 0.0062 0 ------------------------------------------------------------------------------------------------------------- m 3 -- ----- -- -- ----- --- ---- --- ---- - F 3 ---- --- -- ---- --- ---- --- NUMBER IN GROUP: 5 5 5 5 NUMBER IN GROUP: 5 5 5 MEAN: 434.1 0.0305 0.0071 0.0133 MEAN.- 265.7 0.0383 0. STANDARD DEV: 31.4 0.0082 0.0023 0.0032 STANDARD DEV: 16.3 0.0060 0 ------------------------------------------------------------------------------------------------------------ Table 23 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL:WEEKS=ALL DEATH=2;SUBSET=ALL LF ADRENAL - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -- - - - - - - - - - - - - - - - - - - - - - TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL BODY WT ORGAN WEIGHT ORGAD BOD) SEX DOSE BODY WT WEIGHT BODY WT (t) BRAIN WT RATIO SEX DOSE GROUP (g) (g) (I ----G-R-OU-P---------------(-g--)-------(-g-)-.------------------------------------------------------------------------- m 4 F - - -- --- ---- -- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 524.2 44.5 5 0.0330 0.0063 5 0.0063 0.0008 5 0.0146 0.0034 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 5 5 246.7 0.0355 0. 17.5 0.0072 0. ----------------------------------------------------------------------------------------------------------- m 5 - - - - - - - - - - - - - - - - - - - - - - - - - - - - -- - NUMBER IN GROUP: MEAN: STANDARD DEV: 5 470.3 40.1 5 0.0250 0.0032 5 0.0053 0.0004 5 0.0114 0.0005 F _ _5- - - - - - - - - - - - - - - - - - - - NUMBER IN GROUP: MEAN: 5 5 5 249.1 0.0345 0. STANDARD DEV: 26.1 0.0043 0. --------------------------------------------------- ------------------------------------------------------- Table 23 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL RT ADRENAL - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -- - - - - - - - - - - - - - TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGK SEX DOSE BODY Wr WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT HOD ----G-RO-U-P---------------(-g--)-------(-g-)-.--------(-%-)-------RA-T-I-O------------G-RO-U-P---------------(-g-)--------(-g-)------------ m 1 --- --- -- --- ---- --- ---- ---- --- -- F. . .1 . . . . . . . . . . . . . . . . . . . . NUMBER IN GROUP: 5 5 5 5 NUMBER IN GROUP: 5 5 5 MEAN: 495.5 0.0302 0.0062 0.0137 MEAN: 284.0 0..0417 0. STANDARD DEV: 55.9 0.0051 0.0013 0.0021 STANDARD DEV: 39.2 0 0093 0. ------------------------------------------------------------------------------------------------------------- m 2 -- --- --- -- ----- --- --- ---- --- --- F 2 - --- ---- --- ---- -- ---- -- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 481.0 51.3 5 0.0247 0.0094 5 0.0051 0.0016 5 0.0112 0.0043 NUMBER IN GROUP: MEAN- STANDARD DEV: 5 5 5 298.9 0.0322 0. 40.8 0.0056 0. ------------------------------------------------------------------------------------------------------------- m 3 - --- --- --- --- --- ---- ---- -- ---- - p 3 ---- -- ----- -- --- ---- -- - NUMBER IN GROUP: MEAN: STANDARD DEV- 5 434.1 5 0.0286 5 0.0066 5 0.0126 NUMBER IN GROUP: MEAN: 5 5 5 265.7 0.0373 0. 31.4 0.0079 0.0019 0.0034 STANDARD DEV: 16.3 0.0052 0. ------------------------------------------------------------------------------------------------------------- Table 23 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL RT ADRENAL -- -- ---- --- -- --- ---- ---- -- ---- -- -- ----- --- ---- -- ---- -- - TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORG SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BO GROUP (g) (g). (Pd) RATIO GROUP (g) (g) --------------------------------------------- --------------------------------------------------------------- m 4 - ---- --- -- --- ---- --- ---- --- --- - p 4 --- --- ---- --- --- ---- -- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 524.2 44.5 5 0.0318 0.0072 5 0.0060 0.0010 5 0.0140 0.0032 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 5 5 246.7 0.0329 0. 17.5 0.0042 0. ------------------------------------------------------------------------------------------------------------- m 5 --- --- ---- -- --- ---- ---- --- ---- - F 5 --- -- ---- ---- -- --- ---- - NUMBER IN GROUP: 5 5 5 5 NUMBER IN GROUP: 5 5 5 MEAN: 470.3 0.0253 0.0054 0.0115 MEAN: 249.1 0.0323 0. STANDARD DEV: 40.1 0.0044 0.0009 0.0012 STANDARD DEV: 26.1 0.0052 0. ------------------------------------------------------------------------------------------------------------- Table 23 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL BRAIN - ---- -- ---- --- --- ---- ---- --- --- - - ---- -- ---- --- ---- --- --- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BODY GROUP (g) (g). (%) RATIO GROUP (g) (g) (t -------------------------------------------------------------------------------------------------------------- m 1 - ---- -- ---- --- ---- ---- --- ---- -- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 495.5 55.9 5 2.2027 0.0998 5 0.4499 0.0620 5 1.0000 0.0000 F 1 - ---- --- ---- -- --- ---- -- NUMBER IN GROUP: MEAN- 5 5 5 284.0 2.0506 0.7 STANDARD DEV: 39.2 0.0695 0.0 -------------------------------------------------------------------------------------------------------------- m 2 ---- -- --- ---- --- ----- --- --- ---- p 2 - ---- -- -- ---- --- ---- -- NUMBER IN GROUP: 5 5 5 5 NUMBER IN GROUP: 5 5 5 MEAN: 481.0 2.2216 0.4652 1.0000 MEAN: 298.9 2.0992 07 STANDARD DEV- 51.3 0.1022 0.0408 0.0000 STANDARD DEV: 40.8 0.1878 0:1 -------------------------------------------------------------------------------------------------------------- m 3 --- --- -- ---- --- ---- ---- --- --- -- F 3 --- --- --- -- --- ---- --- - NUMBER IN GROUP: 5 5 5 5 NUMBER IN GROUP: 5 5 5 MEAN: 434.1 2.2768 0.5280 1.0000 MEAN: 265.7 2.0240 07 STANDARD DEV: 31.4 0.0980 0.0621 0.0000 STANDARD DEV: 16.3 0.0532 o: ------------------------------------------------------------------------------------------------------------- Table 23 Summary of Organ Weight Data week 14 sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS --------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DF.ATH=2;SUBSET=ALL BRAIN - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - --- - - - - - - - - - - - - - - - - - - - - TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGJ SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BOI GROUP (g) (g). (t) RATIO GROUP (g) (g) ------------------------------------------------------------------------------------------------------------ m- - -4 - - - - - - - - - - - - - - - - - - - - - - - - - - - - F 4 - ---- ---- -- --- -- ----- - NUMBER IN GROUP: MEAN: STANDARD DEV: 5 524.2 44.5 5 2.2740 0.1332 5 0.4366 0.0471 5 1.0000 0.0000 NUMBER IN GROUP: MEAN: STANDARD DEV* 5 5 5 246.7 2.0631 0 17.5 0.0817 0 ------------------------------------------------------------------------------------------------------------ m 5 --- --- --- ----- -- ---- --- ---- --- - F 5 ----- -- ---- --- -- ----- - NUMBER IN GROUP: MEAN: 5 470.3 5 2.1976 5 0.4655 5 1.0000 NUMBER IN GROUP: MEAN: 5 5 5 249.1 2.0375 0 STANDARD DEV: 40.1 0.2136 0.0318 0.0000 STANDARD DEV: 26.1 0.2129 0 ------------------------------------------------------------------------------------------------------------ Table 23 Summary of organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LP KIDNEY --- --- --- --- --- --- ----- --- --- --- -- ---- --- --- --- --- --- -- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGA SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BOU GROUP (g) (g) (t) RATIO GROUP (g) (g) ------------------------------------------------------------------------------------------------------------- N - -1 - - - - - - - - - - - - - - - - - - - - - - - - - - - - NUMBER IN GROUP: MEAN: STANDARD DEV: 5 495.5 55.9 5 1.9158 0.2630 5 0.3867 0.0350 5 0.8733 0.1428 F 1 -- --- ---- --- --- ---- --- - HUM13ER IN GROUP: 5 5 5 MEAN: 284.0 1.1576 0. STANDARD DEV-. 39.2 0.1245 0. ------------------------------------------------------------------------------------------------------------- m 2 -- ---- -- --- ---- -- ----- --- --- --- F 2 -- --- ---- --- --- --- --- -- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 481.0 51.3 5 1.8956 0.2315 5 0.3920 0.0241 5 0.8470 0.0779 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 5 5 298.9 1.1245 0. 40.8 0.1201 0. ------------------------------------------------------------------------------------------------------------- m 3 - - - - - - - - - - - - - - - - - - - - -- - - - - - - - - - p 3 ---- --- --- --- --- ---- --- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 434.1 31.4 5 1.8856 0.1583 5 0.4356 0.0413 5 0.8295 0.0808 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 5 5 265.7 1.1610 0. 16.3 0.0565 0. ------------------------------------------------------------------------------------------------------------- Table 23 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LF KIDNEY - - - - - - - - - -- - - - - - - - - - - - - - - -- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BODY GROUP (g) (g). (%) RATIO GROUP (g) (g) (t -------------------------------------------------------------------------------------------------------------- m 4 - - - - - - - - - - - - - - - - - - - - - - - -- - - - - - - F 4 - --- --- --- --- --- ----- -- NUMBER IN GROUP: 5 5 5 5 NUMBER IN GROUP: 5 5 5 ME": 524.2 2.0510 0.3919 0.9034 MEAN: 246.7 1.1187 0.4 STANDARD DEV: 44.5 0.2638 0.0448 0.1208 STANDARD DEV., 17.5 0.1230 0.0 -------------------------------------------------------------------------------------------------------------- m 5 - - - -- - - - - - - - - - - - - - - - - - - - - - - - - - - F 5 ---- --- --- --- --- ---- --- NUMBER IN GROUP: 5 5 5 5 NUMBER IN GROUP: 5 5 5 MEAN: 470.3 1.9496 0.4174 0.9001 MEAN: 249.1 1.1562 0.4 STANDARD DEV: 40.1 0.1517 0.0525 0.1341 STANDARD DEV: 26.1 0.0619 0.0 -------------------------------------------------------------------------------------------------------------- Table 23 Summary pf organ Weight Data Week 14 sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AMD CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DRATH=2;SUBSET=ALL RT KIDNEY --- --- -- ---- ---- -- --- --- --- ---- - ---- ----- -- ---- --- ---- -- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BODY GROUP (g) (g) (%) RATIO GROUP (g) (g) (%) -------------------------------------------------------------------------------------------------------------- m 1 - - - - - - - - -- - - - - - - - - - - - - - - - - - - - - - NUMBER IN GROUP: MEAN: STANDARD DEV: 5 495.5 55.9 5 1.8989 0.1660 5 0.3849 0.0314 5 0.8647 0.0988 F 1 - ---- --- --- --- ---- ---- - NUMBER IN GROUP: MEAN: STANDARD DEV: 5 5 5 284.0 1.1863 0.4 39.2 0.1066 0.0 -------------------------------------------------------------- m 2 --- --- -- ---- --- -- ---- --- --- --- - NUMBER IN GROUP: MEAN: STANDARD DEV: 5 481.0 51.3 5 1.8373 0.1452 5 0.3834 0.0238 5 0.8262 0.0350 ------- --------------------------------------- F 2 --- ---- --- --- --- ----- -- NUMBER IN GROUP: 5 5 5 MEAN: 298.9 1.1307 0.3 STANDARD DEV: 40.8 0.1396 0.0 -------------------------------------------------------------------------------------------------------------- m 3 -- --- --- -- ---- --- --- --- --- --- -- NUMBER IN GROUP: KEAN: STANDARD DEV: 5 434.1 31.4 5 1.9428 0.1201 5 0.4487 0.0321 5 0.8551 0.0730 F 3 -- --- ---- --- --- ---- -- -- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 5 5 265.7 1.1545 0.4 16.3 0.0706 0.0 -------------------------------------------------------------------------------------------------------------- Table 23 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------ TAJBLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL RT KIDNEY - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -- - - - - - - - TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BODY GROUP (g) (g) (%) RATIO GROUP (g) (g) (t) -------------------------------------------------------------------------------------------------------------- m 4 -- --- -- --- -- ---- ---- --- -- ---- -- F 4 -- --- ----- --- --- ---- --- - NUMBER IN GROUP: MEAN: STANDARD DEV: 5 524.2 44.5 5 2.0560 0.2362 5 0.3930 0.0415 5 0.9070 0.1245 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 246.7 17.5 5 1.1446 0.1468 5 0.46 0.03 --------------------------------------------------------------------------------------------------------------- m 5 -- --- -- --- -- --- --- ---- --- ---- -- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 470.3 40.1 5 1.9357 0.1823 5 0.4152 0.0634 5 0.8945 0.1506 F 5 - --- ---- ----- -- --- --- --- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 249.1 26.1 5 1.1993 0.0960 5 0.48 0.04 --------------------------------------------------------------------------------------------------------------- Table 23 Summary of Organ Weight Data Week 14 sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LIVER - -- --- --- ---- --- ---- -- --- --- ---- ---- --- ---- --- -- --- --- -TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BODY GROUP (g) (g) (t) RATIO GROUP (g) (9) (t --------------------------------- ----------- --------------------------------------------------------------- m 1 - ---- --- -- --- --- --- ---- -- ---- -- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 495.5 55.9 5 15.8246 1.1470 5 3.2105 0.2501 5 7.2089 0.7794 F - - 1 -- - - - - - - - - - - - - - - - - - - - NUMBER IN GROUP: 5 5 5 MEAN: 284.0 9.2939 3.2 STANDARD DEV: 39.2 1.5941 0.2 ---------------------------------------------------- -------------------------------------------------------- N 2 F _ _2- - - - - - - - - - - - - - - - - - - - NUMBER IN GROUP: MEAN: STANDARD DEV: 5 481.0 51.3 5 15.5356 2.6697 5 3.2119 0.2424 5 6.9828 1.1308 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 5 5 298.9 9.1934 3. 40.8 1.2506 0. ----------------------------------------------------------------------------------------------------------- m 3 F 3- - - - - - - - - - - - - - - - - - - NUMBER IN GROUP: MEAN: STANDARD DEV: 5 434.1 31.4 5 14.0264 1.3506 5 3.2296 0.1645 5 6.1829 0.7912 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 5 5 265.7 8.4060 3. 16.3 0.6870 0. ------ ----------------------------------------------------------------------------------------------------- Table 23 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LIVER -- ---- --- -- --- -- ---- --- --- --- --- --- ---- --- ---- -- ----- --- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BODY GROUP (g) (g) (t) RATIO GROUP (g) (g) (t ----------------------------------------------- --------------------------------------------------------------- m 4 - - - - - - - - - - - - - -- - - - - - - - - - - - - - - - - F 4 - - - -- - - - - - - - - - - - - - - - - - - NUMBER IN GROUP: MEAN: STANDARD DEV: 5 524.2 44.5 5 18.8254 3.0304 5 3.5758 0.3075 5 8.3121 1.5632 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 5 5 246.7 8.7074 3.5 17.5 1.0293 0.2 -------------------------------------------------------------------------------------------------------------- m 5 -- --- ---- -- -- ---- --- -- ---- --- -- NUMBER IN GROUP: MEAN: 5 470.3 5 20.3339* 5 4.3329* 5 9.3597* STANDARD DEV: 40.1 2.2244 0.4281 1.3157 F 5 --- -- ---- --- --- L --- ---- NUMBER IN GROUP: MEAN: 5 5 5 249.1 10..5929 4.2 STANDARD DEV: 26.1 0 6685 0.3 -------------------------------------------------------------------------------------------------------------- Table 23 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DRATH=2;SUBSET=ALL LUNG -- -- ---- -- --- --- --- --- ---- --- -- - --- ---- --- ---- ---- --- -- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORG SEX DOSE BODY WT WEIGHT ]BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BO GROUP (g) (g) (%) RATIO GROUP (g) (g) --------------------------------------------- --------------------------------------------------------------- m 1 - - - - - - - - - - - - - - - -- - - - - - - - - - - - - - - F- - -1 - - - - - - - - - - - - - - - - - - - - NUMBER IN GROUP: MEAN: STANDARD DEV: 5 5 5 5 NUMBER IN GROUP: 5 5 5 495.5 1.9239 0.3910 0.8773 MEAN: 284.0 1.4837 0. 55.9 0.1514 0.0406 0.1103 STANDARD DEV: 39.2 0.1221 0. ------------------------------------------------------------------------------------------------------------- m 2 - - - - - - - - - - - - - - - - - - - - - - - - - - -- - - F. . .2 . . . . . . . . . . . . L . . . . . . . NUMBER IN GROUP: MEAN: STANDARD DEV: 5 481.0 51.3 5 1.7946 0.1266 5 0.3760 0.0428 5 0.8099 0.0776 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 5 5. 298.9 1.4581 0 40.8 0.0258 0. ------------------------------------------------------------------------------------------------------------- m 3 --- --- --- -- ---- --- -- --- ---- -- -- F- - -3 - - - - - - - - - - - - - - - - - - - - NUMBER IN GROUP: MEAN: STANDARD DEV: 5 434.1 31.4 5 1.8522 0.2526 5 0.4291 0.0704 5 0.8137 0.1089 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 5 5 265.7 1.2902* 0 16.3 0.1419 0 -------------------------------------------------------------------- ------------ -------------------------- Table 23 Summary of Organ Weight Data Week 14 sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LUNG - - - - - - - - - -- - - - - - -- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BODY GROUP (g) (g) (t) RATIO GROUP (g) (g) (% -------------------------------------------------------------------------------------------------------------- m 4 --- -- -- ---- --- --- ---- -- --- --- -- F 4 --- -- ---- ----- -- ---- --- MU14BER IN GROUP: MEAN: STANDARD DEV: 5 524.2 44.5 5 2.6655 1.5011 5 0.5072 0.2769 5 1.1500 0.5633 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 5 5 246.7 1.3227* 0.5 17.5 0.1112 0.0 -------------------------------------------------------------------------------------------------------------- m 5 --- -- -- ---- --- --- ---- -- -- ---- -- p 5 - - - - - - - -- - - - - - - I - - - - - -- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 470.3 40.1 5 1.7721 0.1713 5 0.3767 0.0158 5 0.8107 0.0351 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 249.1 26.1 5 1.2912* 0.1316 5 05 0:0 -------------------------------------------------------------------------------------------------------------- Table 23 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LF OVARY -- --- --- --- ---- -- ---- --- --- ---- - ---- --- ----- --- ---- --- -- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT SEX DOSE 13ODY WT WEIGHT BODY GROUP (g) (g) (t) RATIO GROUP (g) (g) (% -------------------------------------------------------------------------------------------------------------- m 1 --- --- --- --- --- --- ---- --- --- --- F 1 -- --- --- ---- --- ---- --- - NUMBER IN GROUP: MEAN: STANDARD DEV: 5 495.5 55.9 NUMBER IN GROUP: 5 5 5 MEAN: 284.0 0.0807 0.0 STANDARD DEV: 39.2 0.0147 0.0 -------------------------------------------------------------------------------------------------------------- m 2 - ----- -- ---- --- -- ---- --- --- ---- F 2 - --- --- ---- -- -- - ---- -- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 481.0 51.3 NUMBER IN GROUP: 5 5 5 MEAN: 298.9 0.0707 0.0 STANDARD DEV: 40.8 0.0169 0.0 -------------------------------------------------------------------------------------------------------------- m 3 --- --- -- --- ---- -- ---- --- --- --- - F 3 --- --- ---- --- ---- --- --- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 434.1 31.4 NUMBER IN GROUP: 5 5 5 MEAN: 265.7 0.0674 0.0 STANDARD DEV: 16.3 0.0094 0.0 ------- ------------------------------------------------------------------------------------------------------ Table 23 Summary of Organ Weight Data Week 14 sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SLIBSET=ALL LF OVARY --- -- -- ---- ---- --- -- --- --- --- --- --- --- ---- ---- --- ---- --- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN SEX DOSE BODY Wr WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BODY GROUP (g) (g) (%) RATIO GROUP (g) (g) (% ---------------------------------------------- --------------------------------------------------------------- m 4 --- --- - ---- --- ---- --- --- --- --- - F 4 --- ---- ---- -- --- ---- --- NUMBER IN GROUP: MEAN: STANDARD DEV., 5 524.2 44.5 NUMBER IN GROUP: MEAN: 5 5 5 246.7 0.0768 0.0 STANDARD DEV: 17.5 0.0129 0.0 -------------------------------------------------------------------------------------------------------------- m 5 -- --- -- ---- --- -- ---- --- --- --- -- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 470.3 40.1 F 5 -- --- ---- --- -- - NUMBER IN GROUP: MEAN: STANDARD DEV: 5 249.1 26.1 - - - - - -- 5 5 0.0737 0.0 0.0164 0.0 -------------------------------------------------------------------------------------------------------------- Table 23 Summary of Organ Weight Data week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTAKE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL RT OVARY - - - - - - - - - - - - - - - - - - - -- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BODY GROUP (g) (g) (t) RATIO GROUP (g) (g) (t) --------------------------------------------------------------------------------------------------------------- m 1 --- --- ------ -- --- ---- -- ----- --- F 1 - --- ---- ---- --- --- --- --- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 495.5 55.9 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 284.0 39.2 5 0.0731 0.0060 5 0.02 0.00 --------------------------------------------------------------------------------------------------------------- m 2 --- --- ----- --- --- --- --- ---- --- - p 2 ---- --- --- ----- - ---- --- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 481.0 51.3 NUMBER IN. GROUP: MEAN: 5 5 5 298.9 0.0700 0.0 STANDARD DEV: 40.8 0.0180 0.0 -------------------------------------------------------------------------------------------------------------- m 3 --- -- - ----- --- -- --- ---- ---- --- - F 3 --- --- ---- --- ---- --- -- - NUMBER IN GROUP: MEAN: STANDARD DEV: 5 434.1 31.4 NUMBER IN GROUP: 5 5 5 MEAN: 265.7 0.0707 0.0 STANDARD DLIV; 16.3 0.0154 0.0 ------------------------------------------------------------- ----------------------------------------------- Table 23 Summary of organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL RT OVARY --- -- --- --- ----- -- --- -- ---- --- -- ---- --- ----- -- ---- ---- -- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BODY GROUP (g) (g) (t) RATIO GROUP (g) (g) (%) -------------------------------------------------------------------------------------------------------------- m 4 - -- --- ---- -- ---- --- --- -- ---- --- F 4 -- --- --- ---- --- ---- ---- NUMBER IN GROUP: MEANSTANDARD DEV: 5 524.2 44.5 NUMBER IN GROUP: 5 5 5 MEAN: 246.7 0.0769 0.0 STANDARD DEV: 17.5 0.0191 0.0 -------------------------------------------------------------------------------------------------------------- m 5 - ---- - ---- --- --- --- --- -- ---- --- F 5 -- --- --- ---- --- - ----- - NUMBER IN GROUP: MEAN: STANDARD DEV- 5 470.3 40.1 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 5 5 249.1 0.0699 0.0 26.1 0.0086 0.0 -------------------------------------------------------------------------------------------------------------- Table 23 Summary of Organ Weight Data week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES, SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL SPLEEN --- --- --- ---- ---- --- -- ---- --- --- --- --- ---- ---- --- ----- - TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGA SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BOD GROUP (g) (g) (t) RATIO GROUP (g) (g) --------------------------------------------- --------------------------------------------------------------- m- - 1 -- - - - - - - - - - - - - - - - - - - - - - - - - - - - F 1 -- ---- ----- -- --- ----- - NUMBER IN GROUP: 5 5 5 5 NUMBER IN GROUP: 5 5 5 MEAN- 495.5 0.7759 0.1575 0.3526 MEAN: 284.0 0.5129 0. STANDARD DEV: 55.9 0.0794 0.0174 0.0364 STANDARD DEV: 39.2 0.0444 0. ------------------------------------------------------------------------------------------------------------- m 2 - --- -- --- ---- -- ---- --- --- --- --- NUMBER IN GROUP: mM - STANDARD DEV: 5 481.0 51.3 5 0.7825 0.1814 5 0.1612 0.0229 5 0.3510 0.0728 F _ _2 - - - - - - - - - - - - - - - - - - - - NUMBER IN GROUP: 5 5 5 MEAN: 298.9 0.5494 0. STANDARD DEV: 40.8 0.1033 0. -------------------------------------------------------------- m 3 - --- -- -- --- ---- -- ---- -- ---- --- - NUMBER IN GROUP: MEAN: STANDARD DEV: 5 434.1 31.4 5 0.7287 0.0928 5 0.1686 0.0250 5 0.3201 0.0404 ---------------------------------------------- F _ _3- - - - - - - - - - - - - - - - - - - - NUMBER IN GROUP: 5 5 5 MEAN: 265.7 0.5110 0. STANDARD DEV: 16.3 0.0445 0. -------------------------------------------------------------- ---------------------------------------------- Table 23 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;StIBSET=ALL SPLEEN ---- --- - ---- --- -- ---- -- --- ---- -- --- --- --- ----- ---- --- --- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BODY (t GROUP (g) (g) (%) RATIO GROUP (g) (g) ---------------------------------------------- --------------------------------------------------------------- m 4 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 524.2 44.5 5 0.7046 0.1067 5 0.1341 0.0120 5 0.3120 0.0619 F 4 --- -- ----- ---- --- ---- -- NUMBER IN GROUP: 5 5 5 MEAN: 246.7 0.4750 01 STANDARD DEV: 17.5 0.0247 0:0 -------------------------------------------------------------------------------------------------------------- m 5 -- --- -- ---- --- --- --- -- --- ---- -- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 470.3 40.1 5 0.7754 0.0826 5 0.1650 0.0135 5 0.3543 0.0103 F 5 - ---- --- ---- -- ----- -- -- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 5 5 249.1 0..4326* 0 26.1 0.0310 o: ------------------------------------------------------------------------------------------------------------- Table 23 Sumnary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LF TESTIS - -- --- -- ---- --- ---- --- --- ---- --- - ---- --- ---- ---- --- --- -- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BODY GROUP (g) (g) (%) RATIO GROUP (g) (g) (% -------------------------------------------------------------------------------------------------------------- m 1 - --- --- --- --- --- ---- --- --- ---- - F 1 - - -- - - - - - - - - - - - - - - - - - - - NUMBER IN GROUP: MEAN: STANDARD DEV: 5 495.5 55.9 5 1.7498 0.2466 5 0.3560 0.0582 5 0.7964 0.1237 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 284.0 39.2 -------------------------------------------------------------------------------------------------------------- m 2 - --- --- -- ---- --- --- ---- --- -- --- F 2 - - - - - - - - - - - -- - - - - - - - - - - NUMBER IN GROUP: MEAN: STANDARD DEV: 5 481.0 51.3 5 1.7146 0.1818 5 0.3581 0.0344 5 0.7711 0.0617 NUMBER IN GROUP: MEAN: STANDARD DEV-. 5 298.9 40.8 -------------------------------------------------------------------------------------------------------------- m 3 -- -- --- -- ---- --- --- --- --- --- --- F 3 -- -- ----- -- --- ---- --- -- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 434.1 31.4 5 1.6809 0.0700 5 0.3887 0.0308 5 0.7394 0.0461 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 265.7 16.3 -------------------------------------------------------------------------------------------------------------- Table 23 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANK SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LF TESTIS --- --- --- -- --- --- ---- -- --- ---- -- ---- -- ----- --- --- ---- --- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BODY ----G-RO-U-P---------------(-g--)-------(-g-)---------(-t-)-------RA-T-I-O------------G-RO-U-P---------------(-g-)-------(-g--)---------(-t-)--- m 4 - - - - - - - - - - - - - - - - - -- - - - - - - - - - - - - F 4 ---- --- ----- --- --- --- --- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 524.2 44.5 5 1.6548 0.1429 5 0.3177 0.0390 5 0.7269 0.0220 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 246.7 17.5 -------------------------------------------------------------------------------------------------------------- m 5 --- --- --- -- --- --- ---- -- --- ---- - F 5 --- --- ---- --- ---- -- ---- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 470.3 40.1 5 1.6454 0.1290 5 0.3504 0.0163 5 0.7561 0.0726 NUMBER IN GROUP: MEAN: STANDARD DEVT 5 249.1 26.1 -------------------------------------------------------------------------------------------------------------- Table 23 Summary qf Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL RT TESTIS - ---- -- --- ---- -- --- ---- --- ---- -- ---- -- ----- --- ---- ---- - TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAI SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BOD GROUP (g) (g) (t) RATIO GROUP (g) (g) ------------------------------------------------------------------------------------------------------------- m 1 --- -- --- ---- -- --- ---- -- ----- --- F 1 - --- --- ----- --- ---- -- -- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 495.5 55.9 5 1.7446 0.2362 5 0.3544 0.0524 5 0.7945 0.1246 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 284.0 39.2 ------------------------------------------------------------------------------------------------------------- m 2 --- -- --- ---- --- -- ---- -- ---- ---- F 2 - --- --- --- ---- - -- --- -- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 481.0 51.3 5 1.6989 0.1610 5 0.3548 0.0310 5 0.7642 0.0531 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 298.9 40.8 ------------------------------------------------------------------------------------------------------------- m 3 --- -- --- ---- --- -- ---- -- --- ---- - F 3 ---- -- ---- ---- --- ---- -- NUMBER IN GROUP: mM : STANDARD DEV: 5 434.1 31.4 5 1.6716 0.0876 5 0.3863 0.0289 5 0.7356 0.0557 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 265.7 16.3 ------------------------------------------------------------------------------------------------------------- Table 23 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DRATH=2;SUBSET=ALL RT TESTIS -- -- --- - ----- -- --- --- --- --- ---- - - ---- --- --- ---- -- ----- -- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BODY GROUP (g) (g) (t) RATIO GROUP (g) (g) (%) ------------------------------------------------ ----------------------------------------------------------------- m 4 - - - - - - - - - - - - -- - - - - - - - - - - - - - - - - - F 4 - --- ---- -- ---- ---- --- --- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 524.2 44.5 5 1.6615 0.1437 5 0.3190 0.0402 5 0.7299 0.0263 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 246.7 17.5 -------------------------------------------------------------------------------------------------------------- m 5 --- --- -- ---- -- --- ---- -- --- ---- - NUMBER IN GROUP: MEAN: STANDARD DEV: 5 470.3 40.1 5 1.6738 0.0932 5 0.3567 0.0134 5 0.7686 0.0513 F 5 ---- -- --- ---- -- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 249.1 26.1 --- ---- -------------------------------------------------------------------------------------------------------------- Table 23 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LF THYROID/PARA - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -- - - - - - - - - - - - - - - - - - - - - - TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE TERMINAL BODY WT ORGAN WEIGHT ORGAN BODY SEX DOSE GROUP BODY WT (g) WEIGHT (g) BODY WT (t) BRAIN WT RATIO GROUP (g) (g) (t ---------------------------------------------- --------------------------------------------------------------- m 1 -- --- -- --- --- --- ---- --- -- ---- -- NUMBER IN GROUP: MEAN- STANDARD DEV: 5 495.5 55.9 5 0.0162 0.0098 5 0.0032 0.0015 5 0.0074 0.0046 p 1 - ----- --- ---- --- -- ----- NUMBER IN GROUP: 5 5 5 MEAN: 284.0 0.0069 0.0 STANDARD DEV: 39.2 0.0015 0.0 -------------------------------------------------------------------------------------------------------------- m 2 - --- --- -- --- ---- -- ---- --- -- ---- F 2 - --- --- ---- --- ---- --- -- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 5 5 5 NUMBER IN GROUP: 5 5 5 481.0 0.0121 0.0025 0.0054 MEAN: 298.9 0.0098 0.0 51.3 0.0025 0.0004 0.0009 STANDARD DEV: 40.8 0.0025 0.0 -------------------------------------------------------------------------------------------------------------- m 3 - --- --- -- -- ---- --- --- -- ---- -- -- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 434.1 31.4 5 0.0140 0.0091 5 0.0033 0.0025 5 0.0060 0.0036 F 3 --- --- --- ---- --- --- --- - NUMBER IN GROUP: 5 5 5 MEAN: 265.7 0.0080 0. STANDARD DEV: 16.3 0.0028 0. ----------------------------------------------------------------------------------------------------------- Table 23 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL LF THYROID/PARA - - - - - - - - - - - -- - - - - - - - - - - - - - - - - - - - - - - - - - - -- - - - - - - - - - - - - - - - TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BODY GROUP (g) (g) (t) RATIO GROUP (9) (g) (t -------------------------------------------------------------------------------------------------------------- m 4 - --- --- --- -- --- ---- --- --- ---- -- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 524.2 44.5 5 0.0143 0.0045 5 0.0028 0.0011 5 0.0063 0.0020 F 4 - ---- --- ---- --- -- ---- -- NUMBER IN GROUP: 5 5 5 MEAN: 246.7 0.0091 0.0 STANDARD DEV: 17.5 0.0008 0.0 -------------------------------------------------------------------------------------------------------------- m 5 - --- --- --- -- --- --- ---- --- --- --- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 470.3 40.1 5 0.0114 0.0016 5 0.0025 0.0005 5 0.0053 0.0013 F. . .5 . . . . . . . . . . . . . . . . . . . . NUMBER IN GROUP: 5 5 5 MEAN: 249.1 0.0077 0.0 STANDARD DEV: 26.1 0.0012 0.0 -------------------------------------------------------------------------------------------------------------- Table 23 Sumnary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL RT THYROID/PARA - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -- - - - - - - - - TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BODY GROUP (g) (g) (%) RATIO GROUP (g) (g) (t ----------------------------------------------- --------------------------------------------------------------- m 1 -- -- --- ---- --- --- --- --- --- ----- p 1 --- ---- ----- -- ---- --- -- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 495.5 55.9 5 0.0171 0.0080 5 0.0034 0.0012 5 0.0078 0.0038 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 5 5 284.0 0.0097 0.0 39.2 0.0033 0.0 ---- 7 ---------------------------------------------------------- m 2 -- -- --- ---- -- --- --- --- ---- --- -- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 481.0 51.3 5 0.0132 0.0038 5 0.0027 0.0006 5 0.0059 0.0016 ----------------------------------------------- F 2 -- --- ----- --- --- ---- - NUMBER IN GROUP: MEAN: STANDARD DEV: 5 298.9 40.8 5 0.0110 0.0024 -- 5 0.0 0.0 ------- ------------ ----------------------------------------------------------------------------------------- m 3 - --- --- -- ---- -- --- ---- -- --- ---- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 434.1 31.4 5 0.0126 0.0017 5 0.0029 0.0005 5 0.0055 0.0007 F 3 ---- --- ---- --- --- ---- -- NUMBER IN GROUP: 5 5 5 MEAN.* 265.7 0.0116 0.0 ---S-T-AN-DA-R-D---D-E-V.--------1-6-.-3------0-.0-0-6-2-------0-.-0 --- --------------------------------------------------------------- Table 23 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL RT THYROID/PARA - --- --- --- -- ---- --- --- -- ---- --- - - ---- ---- --- --- ---- ---- - TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BODY GROUP (g) (g) (%) RATIO GROUP (g) (g) (t -------------------------------------------------------------------------------------------------------------- m 4 - - - - - - - - - - - - - - - - - - -- - - - - - - - - - - - NUMBER IN GROUP: MEAN: STANDARD DEV: 5 524.2 44.5 5 0.0159 0.0058 5 0.0031 0.0013 5 0.0069 0.0023 F 4 - ---- ---- --- ---- --- ---- NUMBER IN GROUP: 5 5 5 MEAN. 246.7 0.0097 0.0 STANDARD DEV: 17.5 0.0013 0.0 -------------------------------------------------------------------------------------------------------------- m 5 -- -- --- --- --- --- -- ---- --- ---- -- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 470.3 40.1 5 0.0104 0.0014 5 0.0022 0.0004 5 0.0048 0.0008 F 5 - ---- --- --- --- - NUMBER IN GROUP: MEAN: STANDARD DEV: 5 249.1 26.1 -- ---- - 5 5 0.0095 0.0 0.0011 0.0 -------------------------------------------------------------------------------------------------------------- Table 23 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL UTERUS - --- --- --- -- --- --- --- --- ---- --- - - ----- -- ----- -- ---- ---- - TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BODY GROUP (g) (g) (t) RATIO GROUP (g) (g) (t) --------------------------------------------------------------------------------------------------------------- m 1 -- --- -- --- -- ---- --- ---- -- --- --- F 1 - ---- ---- ---- --- --- ----- NUMBER IN GROUP: ME": STANDARD DEV: 5 495.5 55.9 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 284.0 39.2 5 0.8644 0.3226 5 0.31 0.14 --------------------------------------------------------------------------------------------------------------- m 2 -- --- -- --- -- --- ---- --- --- --- --- F 2 - - - - - - - - - - - - - - - - - - - - - -- - NUMBER IN GROUP: MEAN: STANDARD DEV: 5 481.0 51.3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 298.9 40.8 5 0.5986 0.1521 5 0.20 0.04 --------------------------------------------------------------------------------------------------------------- m 3 -- --- -- --- -- --- ---- -- --- ---- -- - F 3 ----- -- ---- --- -- ----- --- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 434.1 31.4 NUMBER IN GROUP: MEAN- STANDARD DEV: 5 265.7 16.3 5 0.8368 0.3843 5 0.31 0.13 --------------------------------------------------------------------------------------------------------------- Table 23 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=2;SUBSET=ALL UTERUS ---- -- -- --- -- --- ---- --- -- ---- -- - -- ---- --- ---- --- ---- -- -- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN SEX DOSE BODY wr WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BODY GROUP (g) (g) (16) RATIO GROUP (g) (g) (% ------------------------------------------------------------- ----------------------------------------------- m 4 - ---- -- - ---- --- -- ---- --- -- ---- - F 4 ---- --- --- --- ---- --- --- NUMBER IN GROUP: MEAN: STANDARD DEV: 5 524.2 44.5 NUMBER IN GROUP: 5 5 s MEAN: 246.7 0.9249 0.3 STANDARD DEV. 17.5 0.3717 0.1 -------------------------------------------------------------------------------------------------------------- m 5 -- --- -- -- --- --- -- ---- --- -- ---- - F 5 - - - - - - - - - - - - --- - - - - - - - - - NUMBER IN GROUP: MEAN: STANDARD DEV: 5 470.3 40.1 NUMBER IN GROUP: 5 5 5 MEAN: 249.1 0.7984 0.3 STANDARD DEV: 26.1 0.4389 0.1 -------------------------------------------------------------------------------------------------------------- TABLE 24 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CHF60NIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX--ALL;GROUP=ALL;WEEKS=ALL DRATH=3;SUBSET--ALL LF ADRENAL - - - - - - - - - - - - - - - - - - - - - - - - - - - -- - - - - - - - - - - - - - - - - - - - - - - - - - - - TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN SEX DOSE BODY Wr WEIGHT BODY WT BRAIN WT SEX DOSE BODY Wr WEIGHT BODY GROUP to) to) (%) RATIO GROUP (g) to) (% -------------------------------------------------------------------------------------------------------------- m 1 -- ---- --- -- --- --- ----- --- --- --- F 1 --- --- --- ---- ----- ---- - NUMBER IN GROUP: MEAN: STANDARD DEV: 9 713.4 105.5 9 0.0313 0.0076 9 0.0044 0.0010 9 0.0135 0.0036 NUMBER IN GROUP: YMM: STANDARD DEV: 10 414.5 101.9 10 0.0501 0.0290 10 0.0 0.0 -------------------------------------------------------------------------------------------------------------- m 5 ---- --- -- -- --- ---- ---- --- --- --- NUMBER IN GROUP: MEAN: STANDARD DEV: 9 668.6 142.5 9 0.0263 0.0046 9 0.0040 0.0008 9 0.0112 0.0018 p 5 - - - - - - - - - - - - - - - - -- - - - - - NUMBER IN GROUP: MEAN: STANDARD DEV: 10 331.9* 39.7 10 0.0311* 0.0053 10 0.0 0.0 -------------------------------------------------------------------------------------------------------------- Table 24 Summary of organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUSSET=ALL RT ADRENAL -- ---- - ----- --- ---- --- -- ---- ---- --- --- ---- ----- ---- --- -- TERMINAL ORGAN ORGAN-TO- ORGA.N-TO- TERMINAL ORGAN ORGAN- SEX DOSE BODY Wr WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BODY GROUP (g) (g) (%) RATIO GROUP (g) (o) 1%) --------------------------------------------------------------------------------------------------------------- m 1 --- -- --- ----- --- --- --- --- ---- -- F 1 - -- --- ---- ---- ----- ---- - NUMBER IN GROUP: MEAN: STANDARD DEV: 9 713.4 105.5 9 0.0275 0.0060 9 0.0039 0.0010 9 0.0119 0.0028 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 414.5 101.9 10 0.0368 0.0072 10 0.00 0.00 --------------------------------------------------------------------------------------------------------------- m 5 - - - - - - - - - - - - - - - - - - - - - - - -- - - - - - - NUMBER IN GROUP: MEAN: STANDARD DEV: 9 668.6 142.5 9 0.0262 0.0031 9 0.0040 0.0007 9 0.0111 0.0011 F 5 --- --- ---- --- --r-- --- --- NUMBER IN GROUP: MEAN: STANDARD DEV: 10 331.9* 39.7 10 0.0318 0.0037 10 0.00 0.00 --------------------------------------------------------------------------------------------------------------- Talble 24 Summary of organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL:GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET--ALL BRAIN ---- -- ---- --- ---- ---- -- ----- -- -- - ----- ----- ---- --- ---- -- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN SEX DOSE BODY Wr WEIGHT BODY WT BRAIN WT SEX DOSE BODY Wr WEIGHT BODY GROUP (g) (g) (%) RATIO GROUP (g) (g) (% -------------------------------------------------------------------------------------------------------------- m 1 -- --- -- ---- --- ----- -- --- ---- --- F 1 -- --- ------ --- ---- ----- NUMBER IN GROUP: MEAN: STANDARD DEV: 9 713.4 105.5 9 2.3308 0.1344, 9 0.3320 0.0442 9 1.0000 0.0000 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 414.5 101.9 10 2.1293 0.0884 10 0.5 0.1 -------------------------------------------------------------------------------------------------------------- m 5 -- --- -- ---- --- ---- --- --- --- --- - NUMBER IN GROUP: MEAN: STANDARD DEV: 9 668.6 142.5 9 2.3485 0.0730 9 0.3644 0.0722 9 1.0000 0.0000 F s - - - - - - - - - - - - - - -- - - - - - - - NUMBER IN GROUP: MEAN: STANDARD DEV: 10 331.9* 39.7 10 2.2148 0.1036 10 0.6 0.0 -------------------------------------------------------------------------------------------------------------- Table 24 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTAKE SULPONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;CROUP=ALL;WEF.KS=ALL DRATH=3;SUBSET=ALL LF KIDNEY - - - - -- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN SEX DOSE BODY WT WEIGHT BODY WT BRAIN Wr SEX DOSE BODY WT WEIGHT BODY GROUP (g) (g) (%) RATIO GROUP (g) (g) (% -------------------------------------------------------------------------------------------------------------- m 1 - - - - - - - - -- - - - - - - - - - - - - - - - - - - - - - F 1 --- --- ---- ---- ----- ---- NUMBER IN GROUP: MEAN: STANDARD DEV: 9 713.4 105.5 9 2.3449 0.1955 9 0.3327 0.0397 9 1.0078 0.0857 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 414.5 101.9 10 1.3621 0.2284 10 0.3 0.0 -------------------------------------------------------------------------------------------------------------- m s - - - - - - - - -- - - - - - - - - - - - - - - - - - - - - - NUMBER IN GROUP: MEAN: STANDARD DEV: 9 668.6 142.5 9 2.4193 0.2614 9 0.3712 0.0597 9 1.0322 0.1260 F 5 - - - - - - - - - - - - - --- - - - - - - - - NUMBER IN GROUP: MEAN: 10 10 10 331.9* 1.3638 0. STANDARD DEV: 39.7 0.1321 0. ------------------------------------------------------------------------------------------------------------- Table 24 Sunnary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET--ALL RT KIDNEY - --- --- --- ---- -- ----- -- ---- ---- - -- --- ----- ---- ---- ----- - TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN SEX DOSE BODY Wr WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BODY GROUP (g) (g) (%) RATIO GROUP (u) (g) (% -------------------------------------------------------------------------------------------------------------- m 1 p 1 --- -- ----- ---- ---- ----- NU-MBE-R - -IN -GR-OUP-: - - - - -9 - - - -9 - - - - - -9 - - - - - -9 - - - MEAN: 713.4 2.3577 0.3340 1.0129 STANDARD DEV: 105.5 0.2008 0.0348 0.0842 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 414.5 101.9 10 1.4200 0.2361 10 0.3 0.0 -------------------------------------------------------------------------------------------------------------- m 5 - -- --- -- --- ---- ----- -- --- ---- -- F 5 --- ---- --- ---- ---- ----- NUMBER IN GROUP: MEAN: STANDARD DEV: 9 668.6 142.5 9 2.4080 0.2312 9 0.3690 0.0529 9 1.0271 0.1109 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 331.9* 39.7 10 1.3769 0.1375 10 0.4 0.0 -------------------------------------------------------------------------------------------------------------- Table 24 Summary Qf organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DL7ATH=3;SUBSBT=ALL LIVER --- -- -- ---- --- --- ---- --- --- ---- - -- --- --- ---- ----- ----- -- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT SEX DOSE BODY wr WEIGHT BODY GROUP (g) (g) (t) RATIO GROUP lg) (g) (%) --------------------------------------------------------------------------------------------------------------- m 1 - -- --- -- --- --- ---- ---- --- ---- -- NUMBER IN GROUP: MEAN: STANDARD DEV: 9 713.4 105.5 9 20.027S 3.0980 9 2.8113 0.2044 9 8.6130 1.40S4 F 1 --- -- --- ----- ----- ---- -- NUMBER IN GROUP: MEAN: STANDARD DEV: 10 414.5 101.9 10 11.5769 3.0344 10 2.80 0.31 --------------------------------------------------------------------------------------------------------------- m s --- --- -- --- ---- --- ---- --- --- --- F 5 --- -- --- ----- --- ---- ---- NUMBER IN GROUP: MEAN-. STANDARD DEV: 9 669.6 142.5 9 26.6325* 5.5590 9 4.0039* 0.5449 9 11.3659* 2.4751 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 331.9* 39.7 10 13.8907 1.BS68 10 4.20 O.Si --------------------------------------------------------------------------------------------------------------- Table 24 Sunnary of Organ Weight Data Week 53 Sactifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL LUNG - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -- - - - - - - - - - - - - - - - - - - - - - - - - TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN SF.X DOSE BODY Wr WEIGHT BODY Wr BRAIN Wr SEX DOSE BODY Wr WEIGHT BODY GROUP (g) (g) (%) RATIO GROUP (g) (g) 4% -------------------------------------------------------------------------------------------------------------- m 1 --- -- -- ---- ---- --- --- --- ---- --- NUMBER IN GROUP: MEAN: STANDARD DEV: 9 713.4 105.5 9 2.1599 0.2545 9 0.3061 0.0413 9 0.9293 0.1174 F I -- --- ---- --- ---- ----- -- NUMBER IN GROUP: 10 10 10 MEAN: 414.5 1.6819 0.4 STANDARD DEV: 101.9 0.2502 0.0 -------------------------------------------------------------------------------------------------------------- m 5 - -- -- -- ---- ---- --- -- ---- ---- --- NUMBER IN GROUP: MEAN: STANDARD DEV: 9 668.6 142.5 9 2.0477 0.1879 9 0.3151 O.OS24 9 0.8719 0.0758 p 5 - ---- ---- --- ---- --- ---- NUMBER IN GROUP: MEAN: STANDARD DEV: 10 331.9* 39.7 10 1.5740 0.0922 10 0.4 0.0 -------------------------------------------------------------------------------------------------------------- Table 24 Sunnary of organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET--ALL LP OVARY -- --- -- ---- ---- -- ---- ----- -- ---- -- ----- --- ----- --- --- --- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BODY GROUP (g) (g) (%) RATIO GROUP (g) (g) (%) --------------------------------------------------------------------------------------------------------------- m 1 - --- -- -- ---- --- ---- -- ---- ---- -- F 1 - - - - - - -- - - - - - - - - - - - - - - - - NUMBER IN GROUP: MEAN: STANDARD DEV: 9 713.4 105.5 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 414.S 101.9 10 0.0562 0.0192 10 0.01 0.00 --------------------------------------------------------------------------------------------------------------- m 5 - --- -- --- --- ---- --- -- ---- ----- - F 5 --- --- ----- --- ---- --- --- NUMBER IN GROUP: MEAN: STANDARD DEV: 9 668.6 142.5 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 331.9* 39.7 10 0.0557 0.0123 10 0.01 0.00 --------------------------------------------------------------------------------------------------------------- Table 24 Summary of organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL RT OVARY -- --- -- ---- --- --- ---- ---- --- --- - -- ----- ---- ----- ---- ---- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAI SEX DOSE BODY WT WEIGHT BODY WT BRAIN Wr SEX DOSE BODY WT WEIGHT BOD GROUP (g) ig) (t) RATIO GROUP (g) (g) t ------------------------------------------------------------------------------------------------------------- m 1 - - - - - - - - -- - - - - - - - - - - - - - - - - - - - - - F 1 ---- ---- ---- ----- ---- -- NUMBER IN GROUP: MEAN: STANDARD DEV: 9 713.4 IOS.5 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 10 10 414.5 0.1357 0. 101.9 0.2404 0. ------------------------------------------------------------------------------------------------------------- m 5 - - - - - - - - -- - - - - - - - - - - - - - - - - - - - - - NUMBER IN GROUP: MEAN: 9 668.6 STANDARD DEV: 142.5 F 5 --- ---- --- ----- ---- ---- NUMBER IN GROUP: 10 10 10 MEAN: 331.9* 0.0561 0. STANDARD DEV: 39.7 0.0129 0. ------------------------------------------------------------------------------------------------------------- Table 24 Sumnary (?f organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SFX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET--ALL SPLEEN -- --- --- -- --- ---- ---- --- -- --- -- - --- ---- ---- ----- ----- --- TERMINAL ORGAN ORGAN-TD- ORGAN-TO- TERMINAL ORGAN ORGAN- SFX DOSE BODY WT WEIGHT BODY WT BRAIN Wr SEX DOSE BODY Wr WEIGHT BODY GROUP (g) (g) (%) RATIO GROUP (g) (g) (t) --------------------------------------------------------------------------------------------------------------- m 1 - ---- --- -- --- ---- ---- --- -- --- -- NUMBER IN GROUP: MEAN: STANDARD DEV: 9 713.4 105.5 9 0.9792 0.0975 9 0.1382 0.0100 9 0.4208 0.0427 F- - -I- - - - - - - - - - - - - - - - - - - - NUMBER IN GROUP: MEAN: STANDARD DEV: 10 414.5 101.9 10 0.5577 0.1021 10 0.1 0.0 -------------------------------------------------------------------------------------------------------------- m 5 - - - - - - - - - - - - - - - -- - - - - - - - - - - - - - - NUMBER IN GROUP: MEAN: STANDARD DEV: 9 668.6 142.5 9 0.8287* 0.1514 9 0.1252* 0.0133 9 0.3529* 0.0635 F 5 -- ---- ---- --- ---- ----- - NUMBER IN GROUP: MEAN: STANDARD DEV: 10 331.9* 39.7 10 0.5491 0.1344 10 0.1 0.0 -------------------------------------------------------------------------------------------------------------- Table 24 Sununary of organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTAME SULPONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET--ALL LF TESTIS -- -- --- - ---- ---- --- ---- --- --- --- - --- --- ----- ---- ---- ---- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN SEX DOSE BODY Wr WEIGHT BODY wr BRAIN WT SEX DOSE BODY WT WEIGHT BODY GROUP (g) (g) (%) RATIO GROUP (g) (g) (% -------------------------------------------------------------------------------------------------------------- m 1 --- - --- --- ---- --- ---- --- --- ---- p 1 - - - - - - - -- - - - - - - - - - - - - - - NUMBER IN GROUP: MEAN: STANDARD DEV: 9 713.4 105.5 9 1.8551 0.3741 9 0.2641 0.0658 9 0.7968 0.1645 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 414.5 101.9 ---------------------------------------------------------------- ----------------------------------------------- m 5 --- -- -- --- ---- --- ---- --- --- ---- F 5 ---- --- ----- ---- ---- --- NUMBER IN GROUP: MEAN: STANDARD DEV: 9 668.6 142.5 9 1.6414 0.3094 9 0.2531 0.0628 9 0.6995 0.1325 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 331.9* 39.7 -------------------------------------------------------------------------------------------------------------- Table 24 Sumary of organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL,WEEKS=ALL DEATH=3;SUBSET--ALL RT TESTIS - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -- - TERMINAL ORGAN ORGAN-TO- ORGAN-TO- - --- --- ---- ---- 7 ---- ---- TERMINAL ORGAN ORGAN- SEX DOSE BODY wr WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BODY GROUP (o) (g) (%) RATIO GROUP (g) (g) (%) --------------------------------------------------------------------------------------------------------------- m 1 - --- -- --- ---- --- --- ---- ---- --- - F 1 - --- ---- --- ---- ----- ---- NUMBER IN GROUP: MEAN: STANDARD DEV: 9 713.4 105.5 9 1.87S9 0.1801 9 0.2683 0.0491 9 0.8100 0.1189 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 414.5 101.9 --------------------------------------------------------------------------------------------------------------- m 5 - --- -- --- ---- --- --- --- ----- --- - p 5 - --- ---- --- ---- --- ------ NUMBER IN GROUP: MEAN: STANDARD DEV: 9 668.6 142.5 9 1.6551 0.2786 9 0.2560 0.0631 9 0.7054 0.1203 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 331.9* 39.7 -------------------------------------------------------------------------------------------------------------- Table 24 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL LP THYROID/PARA - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -- - - - - - - - - - - - - - - - - - - - - - TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN SEX DOSE BODY WT WEIGHT BODY Wr BRAIN WT SEX DOSE BODY wr WEIGHT BODY GROUP (g) (g) (%) RATIO GROUP (g) (g) (% -------------------------------------------------------------------------------------------------------------- m 1 -- --- -- ---- --- ---- --- ---- ---- -- NUMBER IN GROUP: MEAN: STANDARD DEV: 9 713.4 105.5 9 0.0246 0.0060 9 0.0035 0.0008 9 0.0106 0.0027 p I - - -- - - - - - - - - - - - - - - - - - - - NUMBER IN GROUP: MEAN: STANDARD DEV: 10 414.5 101.9 10 0.0136 0.0062 10 0.0 0.0 -------------------------------------------------------------------------------------------------------------- m 5 --- -- -- ---- --- ---- --- ---- --- --- p 5 -- ---- --- ----- -- ----- -- NUMBER IN GROUP: MEAN: STANDARD DEV: 9 668.6 142.5 9 0.0195* 0.0041 9 0.0029 0.0004 9 0.0083* 0.0018 NUMBER IN GROUP- MEAN: STANDARD DEV: 10 331.9* 39.7 10 0.0115 0.0036 10 0.0 O..o -------------------------------------------------------------------------------------------------------------- Table 24 Summary gf Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXIC17Y AND CARCINOGENICITY STUDY WITH PERPLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DRATH=3;SUBSET=ALL RT THYROID/PARA - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -- - - - - - - - - - - TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN- SEX DOSE BODY WT WEIGHT BODY Wr BRAIN Wr SEX DOSE BODY Wr WEIGHT BODY GROUP (g) (u) (%) RATIO GROUP (g) (g) (%) --------------------------------------------------------------------------------------------------------------- m 1 --- ---- -- -- --- --- ---- --- ---- --- F 1 - ---- ----- --- ---- ---- --- NUMBER IN GROUP: MEAN: STANDARD DEV: 9 713.4 105.5 9 0.0249 0.0048 9 0.0035 0.0007 9 0.0107 0.0018 NUMBER IN GROUP: MEAN: STA14DARD DEV: 10 414.5 101.9 10 0.0148 0.0039 10 0.00 0.00 --------------------------------------------------------------------------------------------------------------- m 5 ---- --- -- -- --- --- ---- --- ---- --- NUMBER IN GROUP: 9 9 9 9 MEAN: STANDARD DEV: 668.6 142.5 0.0240 0.0093 0.0036 0.0013 0.0102 0.0040 F 5 - - - - - - - - -- - - - - - - - - - - - - - - NUMBER IN GROUP: MEAN: STANDARD DEV: 10 331.9* 39.7 10 0.0124 0.0046 10 0.00 0.00 --------------------------------------------------------------------------------------------------------------- Table 24 Summary of organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SF.X=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL UTERUS - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -- - - - - - - - - - - TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN SEX DOSE BODY WT WEIGHT BODY WT BRAIN Wr SEX DOSE BODY WT WEIGHT BODY GROUP (g) (g) (%) RATIO GROUP (g) (v) it -------------------------------------------------------------------------------------------------------------- m I -- --- --- -- ---- --- --- --- ---- -- -- NUMBER IN GROUP: MEAN: STANDARD DEV: 9 713.4 105.5 F 1 - ---- --- ----- ---- --- --- NUMBER IN GROUP: MEAN: STANDARD DEV-. 10 414.5 101.9 10 1.0175 0.3207 10 0.2 0.0 -------------------------------------------------------------------------------------------------------------- m 5 -- ---- --- - ---- --- --- --- ---- --- - F 5 - - - - - - - - - - - - - - -- - - - - - - - NUMBER IN GROUP: MEAN: STANDARD DEV: 9 668.6 142.5 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 331.9* 39.7 10 1.0062 0.3090 10 0.3 0.1 -------------------------------------------------------------------------------------------------------------- TABLE 25 Incidence of Macroscopic observations Week 4 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS *DRAFT* ------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=1;SUBSET=ALL N U M B B R - 0 F -A N I N A L S - A SEX: ----------KALE --------- ---------FEMALEGROUP: -1- -2- -3- -4- -5- -1- -2- -3- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ ** TOP OF LIST ** GENERAL COMMENT (GC) ..............................N.UM.B.ER EXAMINED: 5 5 5 5 5 5 5 5 NO MACROSCOPIC END OF LIST LESIONS 5 5 5 5 5 5 5 5 TABLE 26 Incidence of Macroscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS *DRAFT* ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DRATH=2;SUBSET--ALL SEX: GROUP: N U N B E R -0 F A N I M A L S - A ----------HALE --------- ---------FEKALE-1- -2- -3- -4- -5- -1- -2- -3- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------ ** TOP OF LIST ** GENERAL COMMENT (GC) ..............................N.UM.B.ER EXAMINED: 5 5 5 5 5 5 5 s NO MACROSCOPIC LESIONS BONE NARROW SHEAR TAKEN MALOCCLUSION 3 1 4 3 3 2 5 4 4 5 5 5 5 5 s 5 0 0 0 0 1 0 0 0 KIDNEY (KD) ......................................NU.M.B.ER. EXAMINED: NOT REMARKABLE: 5 5 5 5 5 5 5 5 s 4 4 4 4 4 5 5 CYST(S) LARGE PELVIS(ES) GRANULAR MATERIAL UTERUS (UT) ......................................NU.M.B.ER. EXAMINED: NOT REMARKABLE: 0 0 0 0 0 1 a 0 0 1 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 5 5 0 0 a 0 0 3 5 4 LUMEN FILLED WITH FLUID THYMUS (TH) ......................................NU.M.B.ER. EXAMINED: NOT REMARKABLE: 0 0 0 0 0 2 0 1 5 5 5 5 5 5 5 5 4 5 5 5 4 5 5@ s RED FOCUS(I)/AREA(S) 1 0 0 0 1 0 0 0 TABLE 26 Incidence of macroscopic Observations week 14 sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS *DRAFT* ------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=2;SUBSET--ALL N U N B E R - 0 F -A N I M A L S -A SEX: ----------MALE --------- ---------FEMALEGROUP: -1- -2- -3- -4- -5- -1- -2- -3- ORGAN AND KEYWORDIS) OR PHRASE NUMBER: 5 5 5 5 5 5 5 5 ----------------------------------------------------------------------=--- -=- -=- -=- -=- -=- -=- -=- HARDERIAN GLAND (HG) ..............................N.UM.B.ER EXAMINED: NOT REMARKABLE: 5 5 5 5 5 5 5 5 5 4 5 5 5 5 5 5 HISSINGINOT IDENTIFIED 0 1 0 0 0 0 0 0 STOMACH, GL (ST) .................................N.U.M.BE.R EXAMINED: NOT REMARKABLE: 5 5 s 5 5 5 5 5 5 4 5 5 5 4 5 5 DARK FOCUS(I)/AREA(S) 0 1 0 0 0 1 0 0 LN, MANDIBULAR (HN) ...............................N.UM.B.ER EXAMINED: NOT REMARKABLE: 5 5 s 5 5 5 5 5 4 2 5 4 5 5 5 5 DIFFUSELY RED END OF LIST 1 3 0 1 0 0 0 0 TABLE 27 Incidence of Macroscopic Observations week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ---------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;CROUP=ALL;WEEKS=ALL DEATN=3;SUBSET--ALL SEX: GROUP: NUM BBR -0F-AN IMAL S ------------MALE ------------ ---------1- -2- -3- -4- -S- -6- -1- -2- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 9 o 0 0 9 0 10 D ------------------------------------------------------------------------- ** TOP OF LIST ** GENERAL COMMENT (GC) ..............................NU.M.BE.R EXAMINED: 9 0 0 0 9 0 10 0 NO 14ACROSCOPIC LESIONS BONE MARROW SHEAR TAKEN MALOCCLUSION ANIMAL OBESE 5 0 0 0 3 0 1 0 9 0 0 0 9 0 10 0 1 0 0 0 1 0 2 0 2 0 0 0 1 0 0 0 EYE (EY) ........................................N.U.MB.E.R . EXAMINED: NOT REMARKABLE-. 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 BRAIN (BR) .......................................NU.M.BE.R. EXAMINED: NOT REMARKABLE: COMPRESSED (HYPOTHALAKUS) LIVER (LI) .......................................NU.M.BE.R. EXAMINED: NOT REMARKABLE: DIFFUSELY DARK KIDNEY IKD) ......................................NU.M.BE.R. EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 9 0 0 0 9 0 9 0 0 0 0 0 0 0 1 0 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 0 0 0 0 0 0 0 0 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 Table 27 Incidence of Macroscopic observations Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DRATH=3;SUBSET--ALL N U H B E R - 0 F -A N I NA L S SEX: ------------MALE ------------ ---------GROUP: -I- -2- -3- -4- -5- -6- -1- -2- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 9 0 0 0 9 0 10 0 ---------------------------------------------------------------------=---- -=- -=- -=- -=- -=- -=- -=- SPLEEN (SP) .....................................N.U.MB.E.R. EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 CYST(S) 0 0 0 0 0 0 0 0 PANCREAS (PA) ....................................NU.M.BE.R. EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 9 0 0 0 9 0 '9 0 MASS(ES) 0 0 0 0 0 0 1 0 ADRENAL, CORTEX (AC) ..............................NU.M.BE.R EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 9 0 0 0 9 0 9 0 LARGE 0 0 0 0 0 0 1 0 ADRENAL, MEDULLA (KA) .............................N.UM.B.ER EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 LN, MESENTERIC (MS) ...............................NU.M.BE.R EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 UTERUS (UT) ......................................NU.M.BE.R. EXAMINED: NOT REMARKABLE: LUMEN FILLED WITH FLUID CONTINUED ON NMET PAGE 0 0 0 0 0 0 10 0 0 0 0 0 0 0 9 0 0 0 0 0 0 0 0 0 Table 27 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET--ALL N U N B 8 R - 0 P -A N I M A L S -A SEX: ------------KALE ------------ ----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 9 0 0 0 9 0 10 0 ------------------------------------------------------------------------- ** FROM UTERUS PREVIOUS PAGE ** JUT) ......................................NU.M.BE.R. EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 10 0 0 0 0 0 0 0 9 0 THICKENED WALL 0 0 0 0 0 0 1 0 TESTIS (TE) ......................................NU.M.BE.R. EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 0 0 8 0 0 0 8 0 0 0 SMALL 1 0 0 0 1 0 0 0 BONE, FEMUR (FE) .................................N.U.M.BE.R EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 MARROW, FEMUR (FM) ................................NU.M.BE.R EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 MARROW, STERNUM (SE) ..............................N.UM.B.ER EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 BONE, STERNUM (SB) ................................NU.M.BE.R EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 Table 27 Incidence of 14acroscopic observations Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL N U N B E R -0 F - A N I M A L S SEX: ------------KALE ------------ ---------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 9 0 0 0 9 0 10 0 ----------------------------------------------------------------------=--- -=- -=- -=- -=- -=- -=- -=- SPINAL CORD (SC) .................................N.U.M.BE.R EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 THYMUS (TH) ......................................NU.M.BE.R. EXAMINED: NOT REMARKABLE: LARGE GELATINOUS LUNG (LU) .......................................N.U.MB.E.R. EXAMINED: NOT REMARKABLE: LIGHT FOCUS(I)/AREA(S) DARK FOCUS(I)/AREA(S) HEART (HT) ......................................N.U.MB.E.R . EXAMINED: NOT REMARKABLE: 9 0 a 0 9 0 10 0 9 0 0 0 8 0 10 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 9 0 0 0 9 0 10 0 8 0 0 0 9 0 10 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 MUSCLE, SKELETAL (SM) .............................N.U.MB.ER EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 NERVE, SCIATIC (SN) ...............................NU.M.BE.R EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 Table 27 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET--ALL N U NB B R -0 P -A N I M A L S - A SEX: ------------X-KLE ------------ ----------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 9 0 0 0 9 0 10 0 ----------------------------------------------------------------------=--- -=- -=- -=- -=- -=- -=- -=- TRACHEA (TR) .....................................NU.M.BE.R. EXAMINED-. 9 0 0 0 9 0 10 0 NOT REMARKABLE: 9 0 0 0 9 0 10 0 ESOPHAGUS (ES) ...................................N.UM.B.ER. EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 THYROID (TY) .....................................N.UM.B.ER. EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 PARATHYROID (PT) .................................N.U.MB.E.R EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 PITUITARY (PI) ...................................N.U.MB.E.R EXAMINED: NOT REMARKABLE: LARGE DARK FOCUS(I)/AREA(S) RED FOCUS(I)/AREA(S) RAISED AREA(S) CYST(S) HARDERIAN GLAND (HG) ..............................NU.M.BE.R EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 9 0 0 0 8 0 6 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 Table 27 Incidence of Macroscopic observations Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES- SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET--ALL N U N B B R - 0 P -A N I N A L S SEX: ------------MALE ------------ ---------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 9 0 0 0 9 0 10 0 ---------------------------------------------------------------------=---- -=- -=- -=- -=- -=- -=- -=- SALIV GL, MANDIB (SG) .............................N.UM.B.ER EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 STOMACH, GL (ST) .................................N.U.M.BE.R EXAMINED: NOT REMARKABLE: RED FOCUS(I)/AREA(S) THICKENED WALL STOMACH, NONCL (SU) ...............................NU.M.BE.R EXAMINED: NOT REMARKABLE: THICKENED WALL DUODENUM (DU) ....................................N.UM.B.E.R EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 9 0 0 0 7 0 10 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 9 0 0 0 9 0 10 0 9 0 0 0 a 0 10 0 0 0 0 0 1 0 0 0 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 JEJUNUM (JE) .....................................N.UM.B.ER. EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 ILEUM (IL) .......................................NU.M.BE.R. EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 Table 27 Incidence of Macroscopic observations Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL N U M B B R - 0 F -A N I H A L S SEX: ------------MALE ------------ ---------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 9 0 0 0 9 0 10 0 ----------------------------------------------------------------------=---- -=- -=- -=- -=- -=- -=- -=- CECUM (CE) .......................................NU.M.BE.R. EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 COLON (CO) .......................................NU.M.BE.R. EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 RECTUM (RE) ......................................NU.M.BE.R. EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 SKIN (SX) ............................................ NUMBER EXAMINED: NOT REMARKABLE: CRUSTED AREA(S) LIGHT APJFA(S)/FOCUS(l) KAMMMY, FEMALE (MF) ..............................NU.M.BE.R EXAMINED: NOT REMARKABLE: KASS(ES) THICKENED CYST(S) URINARY BLADDER (UB) ..............................NU.M.BE.R EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 9 0 0 0 9 0 8 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 10 0 0 0 0 0 0 0 6 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 2 0 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 Table 27 Incidence of Macroscopic observations Week 53 sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL N U N B E R - 0 P -A N I N A L S SEX: ------------MALE ------------ ---------GROUP: -1- -2- -3- -4- -5- -6- -1- -2- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 9 0 0 0 9 0 10 0 ---------------------------------------------------------------------=---- -=- -=- -=- -=- -=- -=- -=- CERVIX (CV) ......................................NU.M.BE.R. EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 10 0 0 0 0 0 0 0 9 0 KASS(ES) 0 0 0 0 0 0 1 0 OVARY (OV) ......................................N.U.MB.E.R . EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 10 0 0 0 0 0 0 0 9 0 CYST(S) 0 0 0 0 0 0 1 0 VAGINA IVA) ......................................NU.M.BE.R. EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 10 0 0 0 0 0 0 0 10 0 PROSTATE (PR) ....................................NU.M.BE.R. EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 0 0 9 0 0 0 9 0 0 0 SEMINAL VESICLES (SV) .............................NU.M.B.ER EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 0 0 9 0 0 0 9 0 0 0 EPIDIDYMIDES (EP) .................................NU.M.BE.R F-XMINED: NOT REMARKABLE: 9 0 0 0 9 0 0 0 9 0 0 0 9 0 0 0 Table 27 incidence of Macroscopic observations Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------------------------ TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3,SUBSET--ALL SEX: GROUP: N U M BB R - 0 F - A N I N A L S - A -----------M-ALE ----- ------ -----------1- -2- -3- -4- -5- -6- -1- -2- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 9 0 0 0 9 0 10 0 ------------------------------------------------------------------------ CAVITY, THORACIC (TA) .............................NU.M.B.ER EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 TAIL (TI) .......................................N.U.M.BE.R. EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 LN, MANDIBULAR (MN) ...............................NU.M.BE.R EXAMINEDNOT REMARKABLE: DIFFUSELY RED LN, MEDIASTINAL (ML) ..............................NU.M.BE.R EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 9 0 0 0 9 0 8 0 0 0 0 0 0 0 2 0 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 LN, AXILLARY (AX) .................................NU.M.13.ER EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 LN, INGUINAL (IN) .................................NU.M.BE.R EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 LN, OTHER (LN) ...................................N.U.MB.E.R EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 9 0 0 0 9 0 10 0 Table 27 c incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS ---------------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=3;SUBSET=ALL SFX: GROUP: N U H B ER - 0 P - A N I N A L S - A P F ------------ MALE ------------ -----------FENA -I- -2- -3- -4- -5- -6- -1- -2- -3- ORGAN AND KEYWORD(S) OR PHRASE NUMBER: 9 0 0 0 9 0 10 0 0 ------------------------------------------------------------------------- LN, RENAL (RL) ...................................N.UM.B.ER. EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 10 0 0 9 0 0 0 9 0 10 0 0 MAMMARY, MALE (MM) ................................NU.M.BE.R EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 0 0 0 9 0 0 0 9 0 0 0 0 COAGULATING GL END OF LIST (CG) ...............................NU.M.BE.R EXAMINED: NOT REMARKABLE: 9 0 0 0 9 0 0 0 0 9 0 0 0 9 0 0 0 0 TABLE 28 c Incidence of Microscopic observations Week 14 sacrifice 104-WPEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS *DRAFT* --------------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL N U N B B R - 0 F -A N IM A L S - A F SEX: ----------MALE --------- ---------FEMALE---GROUP: -1- -2- -1- -4- -5- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION NUMBER: s 5 5 5 5 5 s 5 5 ------------------------------------------------------------------------- ** TOP OF LIST ** EYE (BY) ........................................N.UM.B.ER.. EXAMINED: NOT REMARKABLE: 5 s 5 5 5 5 5 5 5 5 5 s 5 5 5 5 5 5 BRAIN (BR) ......................................N.U.MB.E.R. EXAMINED: NOT REMARKABLE: 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 5 LIVER (LI) ......................................N.U.MB.E.R. EXAMINED: NOT REMARKABLE: --INFILTRATE, LYMPHOHISTIOCYTIC --HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR --NECROSIS, INDIVIDUAL HEPATOCYTE --VACUOLATION, HEPATOCELLULAR, MIDZONAL / CENTRILOBULAR --VACUOLATION, HEPATOCELLULAR, PERIPORTAL --FOCUS, ALTERED HEPATOCELLULAR, CLEAR CELL --NECROSIS, COAGULATIVE KIDNEY (KD) ......................................NU.M.BE.R. EXAMINED: NOT REMARKABLE. --INFILTRATE, LYMPHOHISTIOCYTIC --DILATATION, PELVIC --MINERkLIZATION, PELVIC --REGENERATION, TUBULAR EPITHEL M --MINERALIZATION, TUBULAR 5 5 5 5 5 5 5 5 5 0 0 0 0 0 2 0 0 0 5 5 5 5 5 3 5 5 5 0 0 0. 5 5 0 0 0 1 2 2 0 1 2 1 2 2 1 0 0 0 3 4 0 1 0 0 2 3 2 0 0 1 0 3 2 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 1 0 5 5 5 5 5 5 5 5 5 0 1 1 2 1 2 5 2 3 4 2 2 3 2 3 0 3 1 0 2 1 1 2 0 0 1 1 0 0 0 0 1 0 0 0 1 1 0 1 3 1 1 0 0 0 0 0 0 0 0 0 0 1 0 Table 28 c incidence of microscopic observations Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS *DRAFT* --------------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL N U M B E R -0 P - A N I M A L S - A P SF.X: ---------M-ALE --------- --------F-EMALE---GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION NUMBER- 55 55555 5s ------------------------------------------------------------------------ SPLEEN (SP) ...................:...................N.U.MB.ER EXAMINED: NOT REMARKABLE: 5 5 5 5 5 5 5 3 3 4 5 4 0 0 55 10 --PIGMENT, INCREASED PANCREAS (PA) .................................NU.M.BE.R..E.X.AMINED: NOT REMARKABLE: 22 55 42 1 0 1 5 5 4 5 5 5 5 5 5 5 5 5 3 4 4 5 5 3 --ATROPHY, ACINAR --INFILTRATE, LYMPHOHISTIOCYTIC --INFILTRATE, MACROPHAGE, PIGMENTED --HYPERPLASIA, ISLET CELLS ADRENAL, CORTEX (AC) ...........................N.U.MB.ER...EXAMINED: NOT REMARKABLE: 0 0 0 2 1 0 0 0 1 0 2 0 2 1 1 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 5 5 5 5 5 5 5 5 5 5 3 5 5 5 4 5 5 4 --VACUOLATION, --UNILATERALLY ADRENAL, MEDULLA FOCAL EXAMINED (MA) ..........................N.U.MB.E.R..EXAMINED: NOT REMARKABLE. 0 1 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 1 5 5 5 5 s 5 s 5 5 5 4 5 5 5 4 5 5 4 --UNILATERALLY EXAMINED 0 1 0 0 0 1 0 0 1 LN, NESENTERIC (MS) ............................N.UM.B.ER...EXAMINED: 5 5545 555 5 NOT REMARKA13LE: 5 55445 s5 5 --HEMORRHAGE 0 0 0 0 1 0 0 0 0 Table 28 Cov Incidence of microscopic observations Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PP. PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS *DRAFT* ---------------------------------------------------------------------------------------------------------------------- N U N B B R - 0 F -A N I N A L S - A F F TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET--ALL SFX: GROUP: ----------MALE --------- ---------FEMALE ------1- -2- -3- -4- -5- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 5 =- -=- -=- -=- -=- -=- -=- -=- -=- ------------------------------------------------------------------------- OVARY (OV) .......................................NU.M.BE.R.. EXAMINED: NOT REMARKABLE: 0 0 0 0 0 5 5 5 5 0 0 0 0 0 5 5 5 5 TESTIS (TE) .....................................N.U.MB.E.R. EXAMINED: NOT REMARKABLE: --ATROPHY/DEGENERATION UTERUS (UT) .....................................N.U.MB.E.R. EXAMINED: NOT REMARKABLE: 5 5 5 5 5 0 0 0 0 4 5 s 5 5 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 BONE, FEMUR (FE) .................................N.UM.B.E.R EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 MARROW, FEMUR (FM) ...............................N.U.MB.E.R EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 MARROW, STERNUM (SE) ..............................NU.M.BE.R EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 BONE, STERNUM (SB) ...............................N.U.MB.E.R EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Table 28 c Incidence of Microscopic observations Week 14 Sacrifice 104-WEEX DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS *DRAFT* --------------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SFX=ALL,GROUP=1,2,3,4,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSRT=ALL N U N B E R - 0 P - A N I M A L S -A P SEX: ----------MALE --------- ---------FEMALE---GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 5 ------------------------------------------------------------------------- SPINAL CORD (SC) ...................................NU.MBER EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 THYMUS (TH) .....................................N.U.MB.E.R. EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 LUNG (LU) .......................................N.UM.B.E.R . EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 HEART (HT) ......................................N.U.MB.E.R. EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 MUSCLE, SKELETAL (SM) .............................NU.M.BE.R EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 NERVE, SCIATIC (SN) ..............................N.U.M.BE.R EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 TRACHEA (TR) .....................................NU.M.BE.R. EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Table 28 c incidence of Microscopic observations Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS *DRAFT* ---------------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DF-ATH=2;FIND=ALL;SUBSET=ALL N U N B E R - 0 F -A N I N A L S - A F F SFX: ---------- MALE --------- ---------FEMALE ----GROUP: -I- -2- -3- -4- -5- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 s 5 s 5 5 5 s 5 ------------------------------------------------------------------------- ESOPHAGUS (ES) ...................................NU.1.4B.E.R EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 THYROID (TY) .....................................NU.M.BE.R. EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PARATHYROID (PT) .................................N.UM.B.E.R EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PITUITARY (PI) ...................................NU.M.B.ER. EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 HARDERIAN GLAND (HG) ..............................NU.M.BE.R EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 SALIV GL, MANDIB (SG) .............................N.UM.B.ER EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 STOMACH, GL (ST) .................................N.U.MB.E.R EXAMINEI): 0 0 0 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 Table 28 Cov Incidence of Microscopic observations Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH P) PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS *DRAFT* ----------------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL NU NBBR -0F -AN IMA LS -AFF SF.X: ---------- MALE --------- --------F-EMALE -----GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 55555 s 5 5 5 ------------------------------------------------------------------------- STOMACH, NONGL (SU) ............................N.U.MB.E.R ..EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 DUODENUM (DU) .................................N.UM.B.ER...EX.AMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 JEJUNUM IJE) ..................................NU.M.BE.R..E.X.AMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 ILEUM (IL) ...................................N.U.MB.E.R..E.XA.MINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 CECUM (CE) ...................................N.U.MB.E.R ..E.XA.MINED: NOT REMARKABLE: 0000 0000 000 000 00 00 COLON (CO) ...................................N.U.MB.E.R ..E.XA.MINED: NOT REMARKABLE: 0000 0000 0 0 0 0 0 0 0 0 0 0 RECTUM (RE) ..................................N.U.MB.E.R..E.XA.MINED: NOT REMARKABLE: 00 00 00 00 00 00 000 000 Table 28 c incidence of Microscopic observations Week 14 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS .*DRAFT* --------------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL SEX: GROUP: N U N B E R - 0 F - A N I MA LS -A P ----------MALE --------- ---------FEKALE----1- -2- -3- -4- -5- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 5 5 5 5 5 5 5 5 ----------------------------------------------------------------------=---- -=- -=- -=- -=- -=- -=- -=- -=- SKIN (SK) .......................................N.U.MB.E.R.. EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 MAMMARY, FEMALE (MF) ..............................NU.M.BE.R EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 URINARY BLADDER (UB) ..............................NU.M.BE.R EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 CERVIX (CV) .....................................N.U.MB.E.R. EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 VAGINA (VA) .....................................N.U.MB.E.R. EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 PROSTATE (PR) ....................................N.UM.B.ER.. EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 SEMINAL VESICLES (SV) .............................NU.M.B.ER EXAMINED: NOT REMARKABLE: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Table 28 c Incidence of Microscopic observations Week 14 sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS *DRAFT* --------------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4,5;WEEKS=ALL DF.ATH=2;FIND=ALL;SUBSET=ALL N U H B ER - 0 P - A N I N A L S -A F SEX: ---------M-ALE --------- --------F-EMALE---GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5555 5 5 5 5 5 ------------------------------------------------------------------------- EPIDIDYMIDES (EP) .................................NU.M.BER EXAMINED: NOT REMARKABLE: 00 00 00 00 000 000 00 00 END OF LIST TABLE 29 incidence of Microscopic observations Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS --------------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3, FIND=A.LL; SUBSET=ALL NU NBER -0F SF.X: --KALE-- -FEMALEGROUP: -1- -5- -1- -5- A NI NA LS AF ORGAN AND FINDING DESCRIPTION NUMBER: 10 ------------------------------------ ----------------------------------7 -- ** TOP OF LIST ** EYE (EY) ........................................N.UM.B.ER.. EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 9 10 10 BRAIN (BR) ......................................N.U.MB.E.R. EXAMINED: 10 NOT REMARKABLE: 10 --COMPRESSION, VENTRAL 0 LIVER (LI) ......................................N.U.MB.E.R . EXAMINED: 10 NOT REMARKABLE: 0 --INFILTRATE, LYMPHOHISTIOCYTIC 9 --HYPERTROPHY, HEPATOCELLULAR, CENTRILOBULAR 0 --NECROSIS, INDIVIDUAL HEPATOCYTE 0 --VACUOLATION, HEPATOCELLULAR, KIDZONAL / CENTRILOBULAR 1 --VACUOLATION, HEPATOCELLULAR, PERIPORTAL 3 --FOCUS, ALTERED HEPATOCELLULAR, CLEAR / EOSINOPHILIC CELL 7 --HYPERPLASIA, BILE DUCT 1 --HYPERTROPHY, HEPATOCELLULAR, PERIPORTAL 1 --INFILTRATE, MACROPHAGE, PIGMENTED 2 --VACUOLATION, HEPATOCELLULAR 3 --HYPERTROPHY, HEPATOCELLULAR 0 --FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC 0 --CYSI,.BILIARY 0 --INF ilON, SUBACUTE 0 --PIGMENT, HEPATOCELLULAR 0 9 10 10 9 9 10 0 1 0 9 10 10 0 2 0 5 6 9 a 0 9 2 3 3 5 0 0 2 2 1 0 0 0 0 2 3 0 0 0 1 0 3 3 3 1 1 0 0 1 0 0 0 0 1 0 2 0 0 0 6 1 Table 29 c Incidence of Microscopic observations Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ---------------------------------------------------------------------------------------------------------------------- TAJBLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET--ALL N UXBE R-0 F AN INA LS A F SEX: --MALE-- -FEMALEGROUP: -I- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 10 9 10 10 --------------------------------7 ---------------------------------------- KIDNEY (KD) ..................................N.U.MB.E.R..E.XA.MINED: 10 NOT REMARKABLE: 0 9 10 10 112 --INFILTRATE, LYMPHOHISTIOCYTIC 0 --DILATATION, PELVIC 0 --MINERALIZATION, PELVIC 1 --REGENERATION, TUBULAR EPITHELIUM 1 --MINERALIZATION, TUBULAR 0 --NEPHROPATHY, CNRONIC PROGRESSIVE 9 --PIGMENT, TUBULAR 0 --HYALINE DROPLETS, TUBULAR EPITHELIUM 0 --INFILTRATE, MACROPHAGE, PIGMENTED 0 SPLEEN (SP) ..................................N.U.MB.E.R..E.XA.MINED: 10 NOT REMARKABLE: 0 101 001 064 000 146 631 211 100 010 9 10 10 2 0 0 --PIGMENT, INCREASED 10 --HEMATOPOIESIS, EXTRAMEDULLARY, INCREASED 6 --FIBROUS TAGS 0 PANCREAS (PA) .................................N.UM.B.ER...EX.AMINED: 10 NOT REMARKABLE: 3 7 10 10 2s2 0 0 1 9 10 10 2 9 7 --ZYMOGEN GRANULES, DECREASED --INFILTRATE, LYMPHOHISTIOCYTIC --INFILTRhTE, MACROPHAGE, PIGMENTED --HYPERPLASIA, ISLET CELLS --ATROPHY, ACINAR CONTINUED ON NEXT PAGE 1 0 0 0 1 0 1 2 s 7 0 1 2 0 0 0 1 1 0 1 Table 29 c Incidence of Microscopic observations Week 53 Sacrifice p 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS ---------------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SF.X=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL NU NBE R-0 F SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- AN INA LS A FF ORGAN AND FINDING DESCRIPTION NUMBER: 10 ------------------------------------ 7 ----------------------------------- - ** FROM PREVIOUS PAGE ** PANCREAS (PA) ....................................NU.M.BE.R. EXAMINED: 10 NOT REMARKABLE: 3 9 10 10 9 10 10 2 9 7 --NECROSIS / INFLAMMATION, ADIPOSE TISSUE 0 ADRENAL, CORTEX (AC) ..............................NU.M.BE.R EXAMINED: 10 NOT PYA4ARKABLE-. 2 0 1 0 9 10 10 2 1 0 --UNILATERALLY --INFILTRATE, --HEMORRHAGE --HYPERTROPHY, --VACUOLATION, --DEGENERATION, --HYPERPLASIA, EXAMINED MACROPHAGE, PIGMENTED CORTICAL DIFFUSE CYSTIC CORTICAL CELL / HEMORRHAGIC 0 0 1 0 6 4 5 8 0 0 1 0 2 3 0 3 0 1 0 0 0 0 4 1 0 0 0 1 ADRENAL, MEDULLA (KA) .............................NU.M.BE.R EXAMINED: 10 9 10 10 NOT REMARKABLE: 10 9 9 9 --UNILATERALLY EXAMINED 0 LN, NESENTERIC (MS) ..............................N.U.M.BE.R EXAMINED: 10 NOT REMARKABLE: 10 0 1 1 9 10 10 8 10 10 --HEMORRHAGE 0 1 0 0 Table 29 c incidence of Microscopic observations Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUORGOCTANE SULFONIC ACID POTASSIUM SALT IN RATS --------------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL NU NBE R-0F SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- AN INA LS A PF ORGAN AND FINDING DESCRIPTION NUMBER: 10 9 10 10 ---------------------------------=-----=- -=- -=- -------------------------------r----- OVARY (OV) ...................................1.4.ME.R...E.XA.MINED: 0 NOT REMARKABLE: 0 0 10 10 0 9 10 --CYST 0 0 1 0 TESTIS (TE) ...................................NU.M.BE.R..E.X.AMINED: 10 9 0 0 NOT REMARKABLE- 8 9 0 0 --ATROPHY/DEGENERATION 2 --GRANULOMA, SPERM 1 BONE, FEMUR IFE) ..............................N.U.MB.E.R..E.XAMINED: 10 NOT REMARKABLE: 10 00 0 00 a 9 10 10 9 10 10 NARROW, FEMUR (FK) .............................N.UM.B.ER..E.XAMINED: 10 NOT REMARKABLE: 5 --HYPOCELLULAR, NARROW 5 --HYPERPLASIA, MYELOID, MARROW 0 MARROW, STERNUM (SE) ...........................N.U.MB.ER...EXAMINED: 10 NOT REMARKABLE: 9 --HYPERPLASIA, MYELOID, KARROW 0 --HYPOCELLULAR, MARROW 1 9 10 10 6 10 8 301 001 9 10 10 9 10 9 0 0 1 0 0 0 Table 29 incidence of Microscopic observations Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH pE"LUoROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ------------------------------------------------------------------------------------------------ N U N B E R -0 F AN I H A L S A F TABLE INCLUDES: SEX--ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND--ALL;SUBSET--ALL SEX: GROUP: --MALE--1- -5- -FEMALE-1- -5- NUMBER: 10 9 10 10 ORGAN AND FINDING DESCRIPTION ------------ -------------------- -=- -=- -=- -=- ------------------------------7-* - NUMBER EXAMINED: 10 BONE, STERNUM (SB) ................................N.O.T. REMARKABLE: 10 9 10 10 9 10 10 NUMBER EXAMINED: 10 SPINAL CORD (SC) ..................................... NOT REMARKABLE: 10 9 10 10 9 10 10 NUMBER EXAMINED: THY14US (TH) ......................................N.O.T..REMARKABLE: --HEMORRHAGE --DEPLETION, LYKPHOCYTIC --M-THYMIC CARCINOMA --CYST NUMBER EXAMINED: LUNG (LU) ........................................N.OT...REMARKABLE: --CONGESTION --EDEMA, PERIVASCULAR --MINERALIZATION, VASCULAR --HEMORRHAGE --INFILTRATE, MACROPHAGE, ALVEOLAR --INFILTRATE, LYMPHOHISTIOCYTIC 7 8 10 9 5 2 7 6 2 3 2 2 2 3 1 0 0 1 0 0 0 0 0 1 10 9 10 10 4 3 4 4 1 0 0 0 3 3 2 2 1 3 1 0 1 1 2 1 0 1 2 4 1 1 3 2 Table 29 Incidence of Microscopic observations Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS --------------------------------------------------------------------------------------------------------------------- TABLE INCLLTDESSFX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET--ALL N UMBE R-0 P A N IH A L S A F SEX: --KALE-- -FEMALEGROUP: -I- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 10 9 10 10 --------------------------------r---------------------------------=-----=- -=- -=- HEART (HT) ...................................N.U.MB.E.R ..E.XA.MINED: 10 NOT REMARKABLE: 7 9 10 10 5 8 10 --CARDIOMYOPATHY 3 --HEMORRHAGE 0 MUSCLE, SKELETAL (SH) ..........................N.U.MB.E.R..EXAMINED: 10 NOT REMARKABLE: 10 4 2 0 1 0 0 9 10 10 9 10 10 NERVE, SCIATIC (SN) ............................N.UM.B.ER...EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 TRACHEA (TR) ..................................NU.M.BE.R..E.X.AMINED: 10 NOT REMARKABLE: 10 ESOPHAGUS (ES) ................................N.U.MB.ER...EX.AMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 9 9 10 9 9 10 THYROID (TY) ..................................NU.M.BE.R..E.X.AMINED: 10 NOT REMARKABLE: 8 --HYPERPLASIA, C-CELL 1 --M-CARCINOKA, FOLLICULAR CELL 1 9 10 10 9 10 10 000 000 Table 29 Incidence of Microscopic observations Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS --------------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SF.X=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET--ALL N U M B E li- 0 F SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- AN IMA LS AF ORGAN AND FINDING DESCRIPTION NUMBER: 10 9 10 10 ----------------------------------7--------------------------------------=- -=- -=- -=- PARATHYROID (PT) .................................N.UM.B.E.R EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 PITUITARY (PI) ...................................NU.M.B.ER. EXAMINED: 10 NOT REMARKABLE: 4 --B-ADENONA 4 --HYPERPLASIA 2 --ANGIECTASIS 1 HARDERIAN GLAND (HG) ..............................NU.M.BE.R EXAMINED: 10 NOT REMARKABLE: 10 --INFILTRATE, LYMPHOHISTIOCYTIC 0 SALIV GL, MANDIB (SG) .............................NU.M.B.ER EXAMINED: 10 NOT PJDLUUCABLE: 10 9 10 10 8 5 5 1 4 2 1 1 4 0 3 3 9 10 10 8 9 9 1 1 1 9 10 10 9 10 10 STOMACH, GL (ST) .................................N.UM.B.ER. EXAMINED-. 10 NOT REMARKABLE: 9 --EDEMA 0 --INFLAMKATION, ACUTE 1 --CONGESTION 0 --EROSION 0 9 10 10 7 8 10 0 1 0 0 2 0 1 0 0 1 0 0 Table 29 incidence of Microscopic observations Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS --------------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL N UNB ER -0F AN INAL S A F SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 10 9 10 10 ----------------------------------7--------------------------------------=- -=- -=- -=- STOMACH, NONGL (SU) ...............................NU.M.BE.R EXAMINED: 10 9 10 10 NOT REMARKABLE: 9 9 10 10 --ACANTHOSIS / HYPERKERATOSIS 1 0 0 0 DUODENUM (DU) ....................................NU.M.BE.R. EXAMINED: 10 9 10 10 NOT REMARKABLE: 10 9 10 10 JEJUNUM (JE) .....................................NU.M.BE.R. EXAMINED: 10 9 10 10 NOT REMARKABLE: 10 9 10 10 ILEUM (IL) ......................................N.U.MB.E.R. EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 CECUM (CE) ......................................N.U.MB.E.R. EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 COLON (CO) ......................................N.U.MB.E.R. EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 10 10 Table 29 c Incidence of Microscopic observations Week 53 Sacrifice PA 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERPLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS ----------------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;CROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET--ALL NUM BE R-0 P SEX: --MALE-- -PEKALEGROUP: -1- -5- -1- -5- AN IMAL S A PF ORGAN AND FINDING DESCRIPTION NUMBER: 10 9 10 10 -------------------------------7--------------------------------------=-----=- -=- -=- RECTUM (RE) ..................................N.U.MB.E.R..E.XA.MINED: 10 NOT REMARKABLE: 10 9 10 10 7 10 10 --PARKSITISM 0 2 0 0 SKIN (SK) ............................................ NUMBER EXAMINED: 10 NOT REMARKABLE: 10 9 10 10 9 a 10 --ACANTHOSIS/HYPERKERATOSIS --INFLAMMATION, CHRONIC-ACTIVE --ULCERATION --HEMORRHAGE --DEGENERATION, MUSCLE, SUBCUTANEOUS 0 0 2 0 0 0 2 0 0 0 2 0 0. 0 1 0 0010 MAMMARY, FEMALE (MP) ...........................N.U.MB.E.R ..EXAMINED: NOT REMARKABLE: 0 0 10 10 0032 --CYST / GALACTOCELE --HYPERPLASIA, GLANDULAR --SECRETORY MATERIAL --M-PIBROSARCOKA 0032 0044 0065 0010 URINARY BLADDER (UB) ...........................N.U.MB.E.R ..EXAMINED. 10 9 10 9 NOT REMARKABLE: 7 7 10 8 --INFILTRATE, LYMPHOHISTIOCYTIC --PROTEINACEOUS MATERIAL, LUMINAL 3 0 0 1 0200 Table 29 c Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WM PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT IN RATS ---------------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET--ALL N U N B E R -0 F SEX: --KALE-- -FEMALEGROUP: -1- -5- -I- -5- AN IMA LS A FF ORGAN AND FINDING DESCRIPTION NUMBER. 10 9 10 10 -------------------------------7----------------------------------------- UTERUS (UT) ...................................NU.M.BE.R..E.XA.MINED: NOT REMARKABLE: 0 0 10 10 0 0 10 --DILATATION CERVIX (CV) ...................................NU.M.BE.R..E.XA.MINED: NOT REMARKABLE: 0 0 0 3 0 0 10 10 0 0 9 10 --CYST, SQUAMOUS VAGINA (VA) ...................................NU.M.BE.R..E.XA.MINED: NOT REMARKABLE: 0 0 1 0 0 0 10 9 0 0 10 9 PROSTATE (PR) .................................N.UM.B.ER...EX.AMINED: 10 9 0 0 NOT REMARKABLE: 4 6 0 0 --INFLAMMATION, CHRONIC-ACTIVE --INFILTRATE, LYMPHOHISTIOCYTIC 5 3 0 0 1 0 0 0 SEMINAL VESICLES (SV) ..........................N.U.MB.E.R..EXAMINED: 10 9 0 0 NOT REMARKABLE: 10 9 0 0 EPIDIDYNIDES (EP) ..............................NU.M.BE.R..E.XAMINED: 10 9 0 0 NOT REMARKABLE: 9700 --IMMATURE SPERM FORMS CONTINUED ON NEXT PAGE 0100 Table 29 c incidence of Microscopic observations Week 53 Sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS --------------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SRX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET=ALL NUM BER -0F SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- AN IMA LS AF P ORGAN AND FINDING DESCRIPTION NUMBER: 10 9 10 10 -------------------------------7----------------------------------------- ** FROM PREVIOUS PAGE ** EPIDIDYMIDES (EP) ................................N.U.MB.E.R EXAMINED: 10 9 0 0 NOT REMARKABLE: 9 7 0 0 --INFILTRATE, LYMPHOHISTIOCYTIC 1 HE14ATO NEOPLASIA (HN) ..........................N.U.MB.E.R ..EXAMINED: 10 NOT REMARKABLE: 10 100 9 10 10 9 10 10 CAUSE OF DEATH (CD) ............................N.UM.B.ER...EXAMINED: 10 NOT REMARKABLE: 0 --SCHEDULED SACRIFICE 10 LN, NEDIASTINAL (ML) ...........................N.U.MB.E.R ..EXAMINED: 0 NOT REMARKABLE: 0 9 10 10 0 0 0 9 10 10 0 0 0 0 0 0 LN, AXILLARY (AX) ..............................NU.M.BE.R..E.XAMINED: 000 0 NOT REMARKABLE: 0 0 0 0 LN, INGUINAL (IN) ..............................NU.M.BE.R..E.XAMINED: NOT REMARKABLE: --INFILTRATE, MACROPRAGE, PIGMENTED --HEMORRHAGE 0 0 1 0 0 0 0 0 0 0 1 0 0 0 1 0 Table 29 c incidence of Microscopic observations Week 53 Sacrifice p 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS ---------------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;PIND=ALL;SUBSET=ALL NU MBER -0 P SEX: --KALE-- -FEMALEGROUP: -1- -5- -1- -5- AN INA LS AFF ORGAN AND FINDING DESCRIPTION NUMBER: 10 9 10 10 --------------------------------7-------------------------------- --- LN, OTHER (LN) ................................N.UM.B.ER...EX.AMINED: 0 000 NOT REMARKABLE: 0000 LN, RENAL (RL) ................................N.U.MB.ER...E.XAMINED: NOT REMARKABLE: 0000 0000 LN, MANDIBULAR (MN) ............................N.U.MB.E.R ..EXAMINED: NOT REMARKABLE: --HYPERPLASIA, PLASMACYTIC --HEMORRHAGE VERTEBRA/MARROW (VT) ...........................N.U.MB.E.R ..EXAMINED: NOT REMARKABLE: 1021 0000 0020 1021 0000 0000 MAMMARY, MALE (MM) .............................N.UM.B.ER...EXAMINED: NOT REMARKABLE: 0000 0000 COAGULATING GL ICG) ............................N.UM.B.ER...EXAMINED: NOT REMARKABLE: 0000 0 0 0 0 Table 29 incidence of Microscopic observations Week 53 sacrifice 104-WEEK DIETARY CHRONIC TOXICITY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULPONIC ACID POTASSIUM SALT IN RATS --------------------------------------------------------------------------------------------------------------------- TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=3;FIND=ALL;SUBSET--ALL NUNB ER- 0F A NI NALS A P SEX: --MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION NUMBER: 10 9 10 10 ----------------------------------7--------------------------------------=- -=- -=- -=- TAIL (TI) .......................................N.U.M.BE.R. EXAMINED: NOT REMARKABLE: 0 a 0 0 0 0 0 0 END OF LIST Covance 6329-183 3M T-6295 io4 WEEK D=ARY Table 30 ResultsofHomogeneity Analyses(ppm) N&ed 3/18/98 cHRoNic To)acrrYAND CARaNOGEMCrrY STMY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS*,T-6295)IN RATS Samples Location Rephcate T-6295 (ppm) 0.5 20 Top Mddle Bottom 1 0.605 21.3 2 0.588 21.4 Mean 0.597(119)' 21.4(107) 1 0.657 23.6 2 0.690 23.5 Mean 0.674(135) 23.6(118) 1 0.601 24.6 2 0.631 20.4 Mean 0.616(123) 22.5(113) a Each valueinparenthesiissthepercentoftheoretical Covance 6329-183 3M T-6295 Table30 (Continued) ResultsofHomogeneity Analyses(ppm) Mxed 4/16/98 104-WEEK DIETARY CHRONIC TOMCITY AND CARCINOGENICRTY STUDY WrM PERFLUOROOCRANE SULFONIC ACM POTASSRJM SALT (PFOS; T-6295)IN RATS SampleLocation Replicate 0.5 T-6295 (ppm) 2 5 20 Top Middle Bottom 1 0.743 2.22 5.36 18.1 2 0.521 2.03 5.48 17.4 3 0.406 2.12 4.04 18.4 Mean 0.557(111)' 2.12(106) 4.96(99.2) 18.0(90.0) 1 0.478 2.28 4.90 19.7 2 0.478 2.01 4.90 19.5 3 0.486 2.09 5.28 21.3 Mean 0.481(96.2) 2.13(107) 5.03(101) 20.2(101) 1 0.955 2.04 4.70 17.6 2 0.519 2.03 5.00 18.7 3 0.359 1.84 4.94 18.4 Mean 0.611(122) 1.97(98.5)4.88(97.6) 18.2(91.0) a Each valueinparenthesiissthepercentoftheoretical Covance 6329-183 3M T-6295 Table30 (Continued) Resultsof HomogeneityAnalyses(ppm) Mxed 6/18/98 104-WEEK DIETARY CHRONIC TOMCTRY AND CARCINOGENIC= STUDY WrM PERFLUOROOCTANF- SULPONIC ACID POTASSIUM SALT (PFOS; T-6295)IN RATS sampieLocation Replicate 0.5 T-6295 (ppm) 2 5 20 Top Topb Middle Bottom 1 2 3 Mean 1 2 3 Mean 1 2 3 Mean 1 2 3 Mean 0.566 2.38 4.87 20.3 1.10 2.71 5.18 18.7 0.955 2.23 4.90 18.6 0.874(175) 2.44(122) 4.98(99.6) 19.2(96.0) 0.483 0.480 0.401 0.455(91.0) - - - 0.617 2.50 5.15 18.3 0.637 2.11 5.43 17.8 0.635 2.05 4.70 18.2 0.630(126) 2.22(111) 5.09(102) 18.1(90.5) 0.640 3.08 5.08 19.4 0.694 2.29 5.12 20.5 0.532 2.41 5.15 18.2 0.622(124) 2.59(130) 5.12(102) 19.4(97.0) a Each valueinparenthesiissthepercentoftheoretical. b Reassay. Covance 6329-183 3M T-6295 Table30 (Continued) ResultsofHomogeneity Analyses(ppm) Mixed 04122/98 104-WEEK DEgTARy CHRONIC TOMCrrY AND CARCINOGENICrrYSlUDY Wrni PERFLUOROOCRANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295)IN RATS Sample Location Replicate T-6295 (ppm) 20 Top Middle Bottom 1 18.9 2 18.4 Mean 18.7(93.5)a 1 20.1 2 19.6 Mean 19.9(99.5) 1 21.1 2 20.1 Mean 20.6(103) a Each valueinparenthesiissthepercentoftheoretical Table 31 Covance 6329-183 3M T-6295 Resultsof StabiliAtnyalyses(ppm) Mixed 3/18/98 104-WEEK DIERARY CHRONIC TOMCrrY AND CARCINOGENICITY STUDY WITH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295)IN RATS StorageConditions Replicate 0.5 T6295 (ppm) 1.0 2.0 20 Inifie 1 0.657 1.15 2.29 23.6 2 0.690 1.51 2.67 23.5 Mean 0.674(135)b 1.33(133) 2.48(124) 23.6(118) 28 days,room temperature 1 0.507 1.11 1.94 20.7 2 0.554 0.937 2.01 20.8 Mean 0.531(106) 1.02(102) 1.98(99.0)20.8(104) a Resultsforthe0.5-and 20- levelswere derivedfrom thesamplecollectefdrom the middle ofthedose preparationfsorhomogeneity analysis. b Each valueintheparenthesiissthepercentoftheoretical Covance 6329-183 3M T-6295 Table31 (Continued) Resultsof StabiliAtnyalyses(ppm) Mxed 4/16/98 104-WEEK DIERARY CHRONIC TOMCRTY AND CARaNOGENICrrY STUDY WrM PERFLUOROOCTANE SULFONIC ACID PCYRASSIFJM SALT (PFOS; T-6295) IN RATS StorageConditions Replicate 0.5 T6295 (ppm) 1.0 2.0 20 Inifie 1 0.478 2.28 4.90 19.7 2 0.478 2.01 4.90 19.5 3 0.486 2.09 5.28 21.3 Mean 0.481 (96.2@ 2.13(107) 5.03(101) 20.2(101) 33 days,room temperature 1 0.527 2.57 5.64 28.4 2 0.553 2.54 5.83 22.1 Mean 0.540(108) 2.56(128) 5.74(115) 25.6(128) a Resultswere derivedfrom thesamplecollectefdrom themiddleof thedose preparationsforhomogeneity analysis b Each valueintheparenthesiissthepercentoftheoretical. Covance 6329-183 3M T-6295 Table32 ResultsofDose PreparatioAnnalysis(ppm) lo4 WEEK DiErARY CHRONIC TO)acrrYAND CARCINOGENICRTY STUDY WMI PERFLUOROOCTANE SULFONIC ACM POTASSIUM SALT (PFOS; T-6295)IN RATS Mix Date Replicate0.5 T-6295 (ppm) 1.0 2.0 20 3/18/98 1 0.657 1.15 2.29 23.6 2 0.690 1.51 2.67 23.5 Mean 0.674(135P 1.33(133) 2.48(124)23.6(118) a Resultsforthe0.5-and 20-levelswere derivedfrom thesample collectefdrom themiddleof thedose preparationfsorhomogeneity analysis. b Each valueintheparenthesiissthepercentof theoretical. Covance 6329-183 3M T-6295 Table32 (Continued) ResultsofDose PreparatioAnnalysis(ppm) 104-WEEK'D]LrrARY CHRONIC TO]A=Y AND CARaNOGEMCrrY STMY WITH PERFLUOROOCTANE SLUZONIC AaD POTASSIUM SALT (PFOS; T-6295)IN RATS Mix Date Replicate0 0.5 T-6295 (ppm) 2.0 5.0 20 4/16/988 1 b 2 b 3 Mean 0.478 2.28 4.90 19.7 0.478 2.01 4.90 19.5 0.486 2.09 5.28 21.3 0.481(96.2y 2.13(107) 5.03(101) 20.2(101) 4/23/98 1 b 2 b Mean - 4/23/98d 1 - 2 - Mean - 4/23/98'- 1 - 2 - Mean - 0.375 2.07 6.54 19.9 0.379 1.76 4.92 21.3 0.377(75.4) 1.92(96.0)5.73(115) 20.6(103) 0.674 - 0.535 - 0.605(121) - 0.780 - 4.78 - 2.78(55.6) - 0.517 - 0.406 - 0.462(92.4) - 4.55 - 6.12 - 5.34(107) - 4/30/98 1 b 2 b Mean - 4/30/98d 1 - 2 - Mean - 0.551 2.12 5.55 22.6 0.478 2.11 6.03 22.1 0.515(103) 2.12(106) 5.79(116) 22.4(112) 5.24 5.24 5.24(105) a Resultsforthe0.5-,2.0-,5.0-and 20-ppm levelwsere derivedfrom thesample collected from themiddleofthedose preparationfsorhomogeneityanalysis. b Below thelimitofquantitati(o<n0.4ppm). c Each valueintheparenthesiissthepercentoftheoretical d Reassay. e RetentiorL Covance 6329-183 3M T-6295 Table32 (continued) ResultsofDose PreparatioAnnalysis(ppm) 104-WEEK DWRARY CHRONIC TOMCrrY AND CARCINOGENICRIY STUDY WrM PERFLUOROOCTANE suLFoNic AaD PoTAssium SALT (pfos;T-6295)IN RATS Mix Date Replicate0 0.5 T-6295 (ppm) 2.0 5.0 20 5nl98 1 0.962 0.406 1.73 4.37 18.2 2 0.942 0.390 1.74 4.31 18.2 Mean 0.952 0.398(79.6y 1.74(87.0)4.34(86.8) 18.2(91.0) 5nl98d 1 1.11 0.202 - - - 2 1.12 0.557 - - - Mean 1.12 0.380(76.0) - - - 5n/98e 1 b 0.476 - - - 2 b 0.347 - - - Mean - 0.412(82.4) - - - 5/14/98 1 b 2 b Mean - 0.474 2.52 5.48 19.8 0.402 2.54 5.06 21.5 0.438(87.6) 2.53(127) 5.27(105) 20.7(104) 5/21/98' 1 2 Mean 5/21/98d 1 2 Mean <0.75 0.157 1.60 4.98 19.6 <0.75 0.349 1.86 6.27 20.9 - 0.253(50.6) 1.73(86.5)5.63(113) 20.3(102) b 0.248 - b 0.326 - 0.287(57.4) - - - 5/28/98 1 b 0.442 1.95 6.19 2 b Mean - 0.453 2.29 6.49 0.448(89.6) 2.12(106) 6.34(127) b Below thelimitofquantitati(o<n0.4ppm). c Each valueintheparenthesiissthepercentoftheoretical. d Reassay. e Retention. f The low standard(0.4ppm) was notusedforthecalibraticounrve. 21.8 22.3 22.1(111) Covance 6329-183 3M T-6295 Table32 (continued) ResultsofDose PreparatioAnnalysis(ppm) 104-VIEEK D]ERARY CHRONIC TOMCrrY AND CARCINOGENICITY STUDY WITH PERFLUOROOCRANE SULFONIC ACH) POTASSIUM SALT (PFOS; T-6295)IN RATS Mix Date Replicate0 0.5 T-6295 (ppm) 2.0 5.0 20 6/4/989 1 b 2 b Me-an - 0.618 1.95 4.91 18.2 0.610 1.66 5.39 20.3 0.614(123)' 1.81(90.5)5.15(103) 19.3(96.5) 6/11/989 1 b 2 b Mean - 0.446 2.25 5.22 19.0 0.539 1.91 4.81 16.9 0.493(98.6) 2.08(104) 5.02(100) 18.0(90.0) 6/18/98" 1 b 2 b 3 Mean 0.617 2.50 5.15 18.3 0.637 2.11 5.43 17.8 0.635 2.05 4.70 18.2 0.630(126) 2.22(111) 5.09(102) 18.1(90.5) 7/16/98 1 b 2 b Mean - 7/16/98d I - 2 - Mean - 0.564 1.60 3.90 17.9 0.519 1.90 4.13 17.4 0.542(108) 1.75(87.5)4.02(80.4)17.7(88.5) 4.76 5.41 - 5.09(102) - 8/13/98 1 b 2 b Mean - 0.478 2.07 5.60 18.9 0.569 2.04 4.95 19.9 .0.524(105) 2.06(103) 5.28(106) 19.4(97.0) a Resultsforthe0.5-,2.0-,5.0-and 20-ppm levelswere derivedfrom thesample collectefdrom themiddleofthedose preparationfsorhomogeneity analysis. b Below theEmitofquantitati(o<n0.4ppm). c Each valueintheparenthesiissthepercentoftheoretical d Reassay. g Reassayed due tounacceptablecalibratiocnurvefororiginaalssays. Covance 6329-183 3M T-6295 Table32 (continued) ResultsofDose PreparatioAnnalysis(ppm) 104-wEEK DIETARY cHRoNic ToiacrrY AND CARaNOGENICrrY STUDY WrM PERFLUOROOCTANE SULFONIC ACTD POTASSIUM SALT (PFOS; T-6295) IN RATS Mix Date Replicate0 0.5 T-6295 (ppm) 2.0 5.0 20 9/10/98 1 b 2 b Mean - 9/10/98d 1 - 2 - Mean - 9/10/98e' I - 2 - Mean - 9/10/98d., 1 - 2 Mean - 9/10/98, 1 - 2 - Mean - 0.477 2.28 1.38(276Y 2.23 4.58 17.1 2.27 4.64 16.8 2.25(113) 4.61(92.2) 17.0(85.0) 0.213 - - - 0.231 - - - 0.222(44.4) - - - 1.09 - - - 1.27 - - - 1.18(236) - - - b - - - b - - - 0.475 - - 0.386 - - 0.431(86.2) - - - 10/8/98 1 b 2 b Mean - '0.836 1.79 5.68 17.9 0.482 1.83 4.90 17.2 0.659(132) 1.81(90.5)5.29(106) 17.6(88-0) b Below theEmitofquantitati(o<n0.4ppm). c Each valueintheparenthesiissthepercentof theoretical. d Reassay. e Retention. f Reassayoftheretention. Covance 6329-183 3M T-6295 Table32 (continued) ResultsofDose PreparatioAnnalysis(ppm) 104-WEEK DIETARY CHRONIC ToiacrlAYND CARCINOGENICITY STUDY WnH PERFLUOROOCTANE SULFONIC ACID POTASSRUM SALT (PFOS; T-6295)IN RATS Mix Date Replicate0 0.5 T-6295 (ppm) 2.0 5.0 20 10/8/98d 1 - 2 - Mean - 10/8/980 1 - 2 - Mean - 10/8/98' 1 - 2 - Mean - 11/5/98 1 b 2 b Mean - 12/3/98 1 b 2 b Mean 12/3/98d 1 2 Mean b - 0.649(130)' - 0.473 1.02 0.747(149) 0.474 0.497 0.486(97.2) - 0.546 0.556 0.551(110) 1.84 2.05 1.95(97.5) 4.33 16.3 4.45 15.4 4.39(87.8)15.9(79.5) b 1.659 3.86 14.6 b 1.51 3.69 16.6 - 1.58(79.0)3.78(75.6)15.6(78.0) 0.607 1.98 0.539 1.69 0.573(115) 1.84(92.0) b Below thelimitofquantitati(o<n0.4ppm). d Reassay. c Each valueintheparenthesiissthepercentoftheoretical. e Retention. f Reassay oftheretention g Group willreassayeddue to unacceptablechromatography. Covance 6329-183 3M T-6295 Table32 (continued) ResultsofDose PreparatioAnnalysis(ppm) 104_WEEK DIETARY CHRONIC T03aCny AND CARCINOGENICITY STUDY WRIH PERFLUOROOCTANE SULFONIC ACID POTASSIUM SALT (PFOS; T-6295)IN RATS Mix Date Replicate0 0.5 T-6295 (ppm) 2.0 5.0 20 12/31/98 1 2 Mean 12/31/98d I 2 Mean 1/28/99 1 2 Mean 1/28/ggd 1 2 Mean 1/28/ggd 2 Mean 2/25/99 1 2 Mean 2125/99d I 2 Mean b 0.339 1.89 4.42 19.0 b 0.246 1.69 4.71 19.4 - 0.293(58.6)c 1.79(89.5)4.57(91.4)19.2(96.0) - 0.442 - 0.422 - 0.432(86.4) - - - b e b e 1.67 4.71 17.6 2.05 4.92 17.1 1.86(93.0)4.82.(96.4)17.4(87.0) b b b b - - - - - 1.498 b - 0.907 2.06 5.67 20.1 0.852 6.79 7.48 20.1 0.880(176) 4.43(222) 6.58(132) 20.1(101) b 0.450 1.64 4.91 b 0.526 2.06 4.56 b 0.488(97.6) 1.85(92.5)4.74(94.8) b Below thelimitofquantitati(o<n0.4ppm). c Each valueintheparenthesiissthepercentoftheoretical d Reassay. e This levelwas outsideofthemodifiedstandardcurveand willbe retested. Covance 6329-183 3M T-6295 Table32 (continued) Resultsof Dose PreparatioAnnalysis(ppm) 104-WEEK DIERARY CHRONIC To)acrTYAN][)CARaNOGENICrIY stmy wnH PERFLUOROOCTANE SULFONIC ACK) POTASSIUM SALT (PFOS;T-6295)IN RAIS Mix Date Replicate0 0.5 T-6295 (ppm) 2.0 5.0 20 3/25/99 1 b 2 b Mean - 0.414(82.8)' 1.91 5.44 18.5 b 2.97 5.59 19.9 - 2.44(122) 5.52(110) 19.2(96.0) 3/25/ggd 1 - 1.63 2.18 2 - 0.702 1.91 Mean - 1.17(234) 2.05(103) - - 4/22/99 1 b 2 b Mean - 4/22/ggd 1 - 2 - Mean - b 1.97 4.73 17.7 b 2.04 5.72 18.8 - 2.01(101) 5.23(105) 18.3(91.5) 0.431 0.405 0.418(83.6) - 5.06 4.90 4.98(99.6) - 5/20/99 1 b 2 b Mean - 0.582 1.76 4.65 17.8 0.531 1.68 4.67 20.1 0.557(111) 1.72(86.0)4.66(93.2)19.0(95.0) b Below thelimitofquantitati(o<n0.4ppm). c Each valueintheparenthesiissthepercentof theoretical d Reassay.