Document a1jGYXObqBmq2bGVXZ6qab4Va

~ K atherine E . Reed, Ph .D . Staff Vice President Certified Mail July 28, 200 6 3 N I Environmental, Health and Safetv Operations 900 Bush A,,enue, Building 1_1_'E-_'6 PO Box 3333 1 St . Paul, MN 55133-3331 651 778 433 1 ^ C- n 2L ; j ;tu~ 3 ConttJIfl$140 69 66 00 e,2 6 - 36 7)~ ~ Document Processing Center EPA East - Room 6428 Attn : Section 8(e) Office of Pollution Prevention and Toxic s CON-rA ',A/ N(; V B US EPA 1200 Pennsylvan ia Avenue NW J l , Washington DC 20460-0001 <-^ RE : TSCA 8(E) SUBSTANTIAL RISK NOTICE ON : PFHS and PFOS ; Supplemental to Sulfonate-based Fluorochemicals, Docket Nos . 8EHQ-1180-374 ; 8EHQ-0381-0394 ; 8EHQ0598-373 Dear Sir/Madam : Preliminary data fr om an on-going study sponsored by 3M that is being conducted by TNO Laboratories in Leiden, Netherlands suggest an effect of perfluorooctanesulfonate (PFOS) and perfluorohexanesulfonate (PFHS) on body weight, food consumption, and serum cholesterol in 15% dietary fat fed E3 Leiden tran sgenic mice in vivo . Cholesterol was reduced at dietary concentrations of 30 and 100 ppm for PFOS and 60 and 200 ppm for PFHS . Food consumption an d body weight were reduced at dietary concentrations of 100 ppm PFOS and 200 ppm PFHS . On removal of the 100 ppm PFOS dosage-group mice from exposure through diet, food consumption and serum cholesterol showed recove ry, but bodyweights remained low . On removal of the 200 ppm PFHS dosage-group mice from exposure through diet, body weight an d food consumption showed recovery , but serum cholesterol remained low . The lowered serum cholesterol in the 200 ppm PFHA dosage-group mice is being observed for recovering in the on-going study . 3M believes that this is the first observation in mice of cholesterol reduction as a result of treatment with PFOS and PFHS, and the first obse rv ations in mice tre ated with PFHS of reductions in body-weight an d food consumption . PFOS and PFHS has previously been shown to have the effect of reducing body-weight and serum cholesterol in rats, an d PFOS has been shown to produce the la tt er effects in monkeys . Therefore, 3M believes this information to be corroborative . A final report will be forwarded to the EPA when it is received from the test facility . If there are questions, please contact Dayna Blomquist (651-736-5413) . Sincerely, Kathe ne E . Reed Staff Vice President, Environmental Health and Safety Operations I ~i' ~ 9 o 11~11 IE~ b ~ 0 1[111111111111 () 0 4 o 11i 1 11 p. 2 c~ f 00 N o ' IIIIt m o<o 0 o~ x ---._,~ r~ -- ~ ..~C,~~; ;,~'_. . -- - ~.- -, - - ,--__ . - _ _ ____ '4::~'_ ---- ---- -- . /~ /~ . =.:~~" ~