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TRADESECRET
StudyTile EVALUATION OF THE BIODEGRADABILITY OF
USING THiEcCuLoOsSeEnD BsOToTrLE TEST
Author John T. Gannon Jr., Ph.D.
Study Completed on
8/18/95
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Report Revisedon
916195
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Performing Laboratory
E. ICendturaPlonRtesdeearNcehmo&ursDevanedloCpmoemnptany AppGlliaesdgowandBusEinnveisrsonCmoemnmtuanlitMyic3r0o1b,ioPl.oOg.y BLoaxbo6r1a0t1ory
Newark, DE 19714-6101
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EM Proj
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LaboratoryProjectID
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AEM Laboratory Report No. 205-95
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Page 1 of 8
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10941480
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DuPont AEMR 205.95
GENERALINFORMATION
MaterialTested:
a
Synonyms/Codes: NONE
`Submitter'sNotehookNo: N/A
Sponsor: FWiLlPlRiam J. Brock
ExperStiarmt&eConmpt letaioln; 7-11-95/ 8-8-95
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EID941481
DuPont AEMR 205-95
CEVALUATIO"N_)OUFSITNHGETHBIEOCDLEOGSREADDABBOITLTILTEY TOEFST
SUMMARY
The test chemical, using the 28-day Closed
Botile Test.
was tested for biodegradability The results of the 28-day Closed
Bottle Test indicated that a peak of 12.1 % Biodegradability was observed
at Day 28. A minimum of 60 % biodegradability is required to pass this
test. In addition, the "pass level" of 60 % must be reached within a 14 day
wthiantd[ow_ after exceedi"nJg thweas10no%t "leRveeal.dilTyherBeifoodreeg,raitdambalye."be cPolnecalseudHeodtTM"
that failure to pass a "Keady Biodegradability TestTM does not imply that fe '
titemste.material will persist in the environment for an extended period of .
i
Study Conducted by: MaladBui iso
`Wallace E. Bessicks
(date)
Microbiology Research Technician
i
Study Conducted by: Aacele,Onmedis~9/6/95
Nicole L. DiMondi
(date)
Senior Microbiology Technician
Study Conducted by: B Georuge e4. FaisheAr xl
oils
(date)
Senior Microbiology Technician
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RAeppporrotvedPrefpoarreIdssuaeBndy
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bb]Roe
ofr_
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Jhn T. Gannon Jr., Ph.D.
Research Microbiologist
(date)
3
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E1D941482
DuPont AEMR 205.95
Introduction/Purpose.
The Closed Bottle Test was performed to determine if
_
be used
for
= "Readily
either water-soluble
Biod
or
ewgartaedra-bilnes"o.lubTleh
e Closed
and vol
a
tBioltetleorTensotn-can
volatile chemicals. In this test, measured and is considered the
the Time-0 DO starting point.
(Dissolved Oxygen) is The Closed Bottle Test
is
a
closed to the
system. Therefore, no system for the duration
aodfditthieonatelst.dissIfolvtheed
oxygen bacteria
smheotualbdolibzee
atdhdeed
test chemical in this closed system, then oxygen. Consequently, biodegradation is
they will measured
use up ss the
the loss
dissolved of dissolved
o28xy>gen60wi%t,hinantdhethcilsosleedveblototlfe.bioIdfegtrhaedaDbiOlit(yDihsassolvbeedenOxaycgheinev)edloswsitahtinDa1y4
cdoanyssidoefreedxc"eReedaidnigly10Bi%odebgiroaddeagbraldea"bilaintdy,nothefnurtthheertetsetstcinhgemiiscalneciesssary.
Materials/Method
.
with
The AEM
CSlOoPsedMIBOoStIt-leP
T"eRsetaddyetBeiromdiengartaidoanbilwiatsy:
cColnodsuecdteBdottilne
aTcecsotr"d.ancTehe
-
WsioulrmciengotfonthePOiTnoWc.ulumSefeorTatbhleeCl1 osfoerd mBaotuerliealTsestteswteads. the City of
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Results/Discussion
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12.1 %Inbtihoedeg2r8a-ddaabyilCiltoyseatd DBaotytl2e8Te(sTta,bl[es_ 2 & 3 and FigJuresh1o).wedThaerpeefaorke,ofit
i
was determined that [__
7) was not "Readily Biodegradable".
i
The control chemical, (Figure 1), therefore the test
Sodium Acetate, exceeded is valid. The Theoretical
60 % biodegradability Oxygen Demand of
C
T) was calculated as 0.66 mg Oz per mg of active substance.
not
imPplleyasethantottehethtaetstfamialtuereriatlo
pass will
paers"iRsetadiyn
tBhieodeegnrvaidraobnimleintyt
Tfeosrt"andoes
extended period of time.
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EID941463
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DuPont AEMR 205-95
cEVALUATIOUNSOIF NTGHETBHIEOCDLEOGSREADDABBOITLTILTEY TOEFST TABLE 1
Mme
Description of Test and Control Substances rm shee mae
_464--11
Sodium Acetate
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DuPont AEMR 205.95
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