Document ZnonYZd2jgEbnLr89mYgJ4Dx0
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ACTIVATED SLUDGE RESPIRATION INHIBITION TEST
TEST SUBSTANCE___________________________
Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-3017. (1Octanesulfonic acid) (CAS # 2795-39-3).
Remarks: The 3M production lot number was not noted. The test sample is FC-3017. Current information indicates FC-3017 is a mixture of 0.67% PFOS, 20% diethylene glycol butyl ether, 55.41% water, 21% ethylene glycol, 1.33% Hydroxy foamer, 1% sodium octyl sulfate, 0.5% polyoxyethylene monooctylphenyl ether, 0.04% sodium lauryl sulfate, and 0.05% tolyltraizole.
The following summary applies to a mixture wjth incompletely characterized concentrations o f impurities. Data may not accurately reflect toxicity o f the fluorochemical component o f the test sample.
M E T H O D ________________________________________________
Method: OECD Guideline 209 GLP: No Year Completed: 1995 Analytical monitoring: Dissolved oxygen concentrations, pH and temperature. Statistical methods: Results were determined by calculation of the % inhibition in respiration rate. Test organism source: Activated sludge mixed liquor collected from the Metro Wastewater Treatment Plant, St. Paul, MN. Test condition:
Dilution water: Millipore Milli-QTM water Synthetic sewage: per OECD method Reference and test solution preparation: A stock solution of the reference substance, 3,5-dichlorophenol, was prepared by dissolving 500 mg in 10mL 1N NaOH, diluted to 30mL with Millipore Milli-QTM water, then brought to the point of incipient precipitation with 1N H2SO4 and diluted to 1L with Milli-QTM water. The pH of the reference solution was measured to be 7.7. Test substance was added directly to the test vessels. Test vessels: Not given. Number of concentrations: one plus blank control, in duplicate Temperature: 18.9 - 20.6 C Total suspended solids and pH on day of testing: 3.8 g/L (TSS in final: 1.5 g/L), initial pH 6.4 and final pH 7.1 Element Basis: Respiration inhibition as determined by oxygen consumption. Remarks: Values corrected to 20C for calculations.
002806
RESULTS Nom inal concentrations: 2 Blank controls, 1000 mg/L plus 10 mg/L concentration for reference substance, all in duplicate. Elem ent values: 30-minute EC50 = >1000 mg/L
3-hour EC50 = >1000 mg/L Element values based on nominal concentrations. Rem arks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS____________________________ _ _________________ The test substance 3-hour EC50 for activated sludge respiration inhibition was determined to be >1000 mg/L, the highest concentration tested. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY___________________________________________________ R eliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, the sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in solution. R E F E R E N C E S __________________________________________ This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number N 3 1 8 8 ,1995. O T H E R _____________________________________________________ Last changed: 6/26/00
002807
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ENVIRONMENTAL LABORATORY ACTIVATEDSLUDGEMIXEDLIQUOR
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OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
TEST SOLUTIONS PREPARATION
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I.OECO ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
Test Substance: .
RAW DATA
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ENVIRONMENTA0LABORATORYJ OECD ACTIVATED SLUDGE RESPIRATION INHIBC ;CALCULATION O F R E S U L T S ^ ^ ^ ^
' Test S u b s ta r c e i^ E t a iilZ ^ B lp
: Analyst: GWJ
mg/L 0.5 1.0
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OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 - ,, . TEST SOLUTIONS PREPARATION
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ENVIRONMENTAL LABORATORY
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION' TEST #209
RAW DATA
Test Substance:
5 " cLU r a r L i *
Source of Activated Sludge Mixed Liquor
/ Date Received: 7 / ; - 3 3
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30-MINUTE EXPOSURE
Test Substance Concentration (m.->fL) 0.5
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DISSOLVED OXYGEN CONCENTRATIONS, mgL
TIME- MINUT ES 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0
Temp 5.5 C
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Test Substance
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Test Substance: 3.5-DICHLOROPHENOL
Analyst: GWJ
30-Mlnute Exposure
Dissolved Oxygen Concentrations, mq/L
mg/L 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5
BK1 7.7 7.5 7.3 7.1 6.8 6.6 6.4 6.0 5.7 5.4 5.2
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Temp. *C 20.2 20.1
R (D 0.5285 0.3745
Ra (2Y % ln h ? (3 )
mm mmsm-
0.5212
,
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0.3720 ! 30
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BK2 7.7 7.5 7.3 7.0 6.7 6.4 6.2 5.9 5.6 5.3 5.1 % diff in controls (4): 5.0
20.0
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0.5479 0.5479 , RaCm (5): .0.5345
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mg/L 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 Tmp, *C R (D Ra (2) % Inh. (3)
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19.3 0.4558 0.4785
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18.9
0.4655 0.5024 : RaCm (5): 0.4905
(t) R- Osygen uptake rat* (mg 02/l-min) i2l Ra R adjusted to 20` C> [R/o.Ex] (31 % Inhibition- |(RaCm)-RaS/(RaCm)|tOO (4) % difference In controls: [IRiBkt-RaBk2l/RaCm] 100 (Two controls must be within 15% o< each other.) IS) Mean adjusted R, oonlrols (RaBktRaBk2y2
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