Document ZnK32LX3G0Oe24bzjp5GQ2KyV
ARRRE -- 3.23]
|
413
.
AR2RG _ 323)
Revised Page
Dupon448%
`TRADE SECRET
H-24616:
Study Tide
Influence on Growth and Growth Rate of the Green Alga Selenastrum capricornutum
Laboratory Project ID: DuPont-4488
AUTHOR: Terry Lee Sloman, B.S.
-
ORIGINAL REPORT COMPLETED: November 16, 2000
REPORT REVISION NUMBER 1: February 13, 2002
PERFORMING LABORATORY: E.L du Pont de Nemours and Company
Haskell Laboratory for Toxicology and Industrial Medicine Elkton Road, P.O. Box 50 Newark, Delaware 19714-0050
work Request Nose EEN
run Coneow{eBnY
Company Saniized. Does not contain TSCA CBI =n
1o.f2t4h6e1G6:reenfAulepnscSeeolneGnraosrwatmhaspnrdicGorrnouwnaRmate
Dupon-4455
Substance
STUDY INFORMATION
|
Synonyms/Codes: +' -- ,H-24616
ro Haskell Number: 24616
Known Impurities]
Physical Charact crisis:[QE
pm) \ 1
`Sponsor: E. 1. du Pont de Nemours and Company Wilmington, Delaware 19898
USA.
`Study InitatedCompleted; November 6, 2000 (sec report cover page)
-
In-Life Initiated/Completed: November 6, 2000/ November 9, 2000
-
_--_------ee--_-- Company Sanitized. Does not contain TSCA CBI
oH-f2t4h6e1G6r:eeInnfAllugeancSeeloennaGsriorwutmhcaanpdriGcroorwntuhtuRnate:
RDeuvPiosnetd4P4a5g8e
SUMMARY
A`grsetenudaylgwaaSsecloenndauscttreudm ctoapdreitceorrmniunteumt.he TefhfeecatlogfaeHw-e2re46ex1opn6ostehdetgornoowmtihnaanldcognrcoewntthrartaitoeonsftohfe 0.01,0.1, 1, 10, 100, and 1000 mgH-24616/Literof nutrient medium (ppm).
e"Txhpereaslsgeadc waserpeerecxepnotsiendhifboirti7o2nhionugrrsow(3thdabyass)ewdiotnhohuetaltetshtymceeldlicuomunrten(aelwsaol.refTehrereedff(e0catswcaesll density) relative to the blank (culture medium) control for the 72-hour (day 3) intervalof the test.
A(Rn-siqnuvaerrsee=qu0.a9d2r)a.tiTchreegersetsisimoant'edwaECss)fi?t
twoasthe87d0atam.g/TLh,ewifitthof95th%e
fmiodduceilaltobotuhenddsaotafw7a3s6
good
mg/L
and 1004 mg/L.
H-24616 exhibited low concer' under TSCA in a 72-hour, acute test using the green alga
Selenastrum capricornutun.
> >
DBrruacpee,r,RINDL.R. Smuchek, 1,
aannddVSemrsietehg., Clements, R,
D.1J9.51.199A2p.plEinevdiRreognrmeesnstiaolnTAonxaliycsoilso,gyWialnedyChemisiry, 11, Morcock, R., and Raber, W. (1993). Assessing ecological
1h4a8z5ar-d14u9n4der
TSCA
-
"{mWetGhodLsanadnids,ev1a5l.uaHtuiognheosf,daatan.d ME.nAv.irLoenwmiesn,tEadlsT)o.xipcpo2l2o-g3y9.andAmReirsikcAasnseSsoscmieentty,fAorSTeTstiSnTgPn1d179M.aterial,
Philadelphia _
Company Sanitized.T Doos noT t containTTSCE A CBI oe
1of.t2h4e61G6r:eeInnfAllugesncSeloennGarsowttrhcsanpdriGcroorwntuhtuRmate:
Dupont4488
CERTIFICATION
1, the undersigned, declare that this report provides an accurate evaluation ofdata obtained from this study.
CoedbyStudy Director: iyTfareSaMonmsooaemnm58
LNoorv-32000
Company Senitized. Does nol contain TSCA CBI ER
o1f.t2h4e61G6r:eeInnfAllugeancSeeloennaGsriorwutmhcaanpdrGircoomwutthuRmate
DuPont4485
RESULTS 0B.a1s,e1d, o1n0,vainsudal10ob0smergv/aLtitoensst,stohluetbiloannskwe(nroercmlaelarcualntdurheamdendoiucoml)orcobnetfroorlesaodlduittiioonnoafnadltghael0c.e0l1l,s Hthoeweadvdeirt,iotnhoef1a0l0ga0lmcge/llLs,tetshtestoesltutainodn bwlaasnkslciognhttrlyolclfoluasdkysbweefroerepladadcietdioonnoafaslhgaaklercetlalsb.le iAnftaer cdahyasm)bweirtwhiotuht ametdemipuemrarteunreewoalf.aplplruomxiinmaattieolnyw2a5sCs.upTphlieedalbgyaecowoelr-ewhiintceubfaltueodrefsocren7t2thuboeusr.s (T3he `remveoalnutliigohntsipnetrenmsiitnyutine t(hpemc).hamTbheerpwHasme8a4s4u0rleummeenntso/fm'th(e=cluulxt)u.reTmheedsihuamkiwnagssp7e.4e5d awtasstu1d1y0 start. The pH measurementsofthe definitive test solutions at test initiation ranged from 6.86 to 7.45, and at test termination ranged from 7.09 to 7.54.
Company Sanitized. Doss not contain TSCA CBI En
oHf2t4h6e16G:reeInnfAllugeancSeeloennGasriorwutmhcaanpdriGcroorwntuhtuRmate
DuPont-4488
--
TABLES
Company Sanitized. Does not contain TSCA CBI --
H-24616; Influence on Growth and Grow Rate oftheGreen Alga Slenastrum capricornutum__________
DuPortdits
TABLE 1
pH MEASUREMENTS OF TEST SOLUTIONS
Nominal
Concentration
mgr) |
Test Initiation
(0 hours)
pn
(72 hours)
| pH
Blank Control
745
7.18
0.01
6.87
7.09
0.1
6.87
7.28
1
6.86
7.38
11000
66.3866
77.2459
1000
6.86
7.54
Company Sanitized. Doss not contain TSCA C81 BE
o1f.t2h4e61G6r:eeInnfAllugeancSeeolnenGasirrum aconapdrGwircoorwtntuhtRuhamte
DuPont 4488
TABLE 2
DATA SUMMARY
Nominal | Concentration
Day
0
Healthy Cell cells/mL)
Count
|
Day
3
Healthy Cell cells/mL)
Count
Blank Control Replicate 1
10,000
Replicate 2
10,000
2,190,000 2,590,000
0.01 mg/L Replicate | Replicate 2
10,000 10,000
2,130,000 2,280,000
0.1 mg/L. Replicate |
10,000
Replicate 2
10,000
2,090,000 2,590,000
-
1 mg/L Replicate |
10,000
2,160,000
Replicate 2
10,000
2270,000
10 mg/L
Replicate | Replicate 2
10,000 10,000
2,340,000 2,490,000
100 mg/L. Replicate |
10,000
Replicate 2
10,000
2,170,000 2,470,000
1000 mg/L.
Replicate | Replicate 2
10,000 10,000
680,000 770,000
Sompany Sanitized. Doss not contain TSCA CBI e-- e ------------------ - ------------------
oHf-t2h46e1G6r:eeInnfAllugeancSeeloennaGsrtorwutmhcaanpdriGcroorwntuhtuRmate
DuPont4488
FIGURES
Company Senilized. Doss not contain TSCA CBI >
FIGUR1E 72-HOUR HEALTHY CELL COUNT DOSE RESPONSE CURVE
TTT XX 100
0
N|
a .
Hof2t4h6e1G6r:eeInnAfllugeancSeloennGarsorwudmhacanpdriGcroorwntuhtuRnate
DuPont-4488
APPENDICES
Sortpany Sanitizad. Doss not contalr TSCA CBI ---
o1f.t2h4e61G6r:eeInnfAllugeancSeeloennGasriorwutmhcaanpdriGcroorwntuhtuRnate
DuPon-4488
_
APPENDIX A
72-Hour Healthy Cell Count
A:mpany Sanilized. Doss nct sontain TSCA CB [0
oHf:t2h4e61G6r:eeInnfAllugeancSeloennaGsrtorwutmhcaanpdriGcroornwuhraRnate
RDeuvPiosnetd4P4a8g5e
(Company Saniized. Doss notcontain TscA op -_--
oHf-t2h4e61G6r:eeInnAfllugeancSeeloennaGsriorwutmhcaanpdriGcroorwniuhtuRmate
RDeuvPiosne-d4P4a8ge8.
--
APPENDIX B
Reason for Revision No. 1
Zompany Sanitized. Does not contain TSCA CBI 7
Hof2t4h6e1G6reeInnAfllguaSeenolcenenaGrowthcaandpGrircoowrnRte
RDeuvpiosnetd4P4a8g5e
REASON FOR REVISION NUMBER 1
The report was revised as follows:
Pages
3 Summary: Recalculationoftest endpoints. 10 Figure 1: Recalculation of test endpoints. 13 Appendix A: Recalculation of test endpoints.
14-15 Appendix B: Reason for Revision No. 1 added.
"The following page(s)s were revised to reflect these changes. The original pages (slong with
those listed above) were removed from the report and are maintained in the Medical Research
file.
Pages
~~
1 Title Page
StudyDirector:
dis"TerryLee Sloman, BS.
3-FEDRu-e2002,
pany Sanitized. Doos not contain TSCA Cal = -_--
