Document Zn9K8Z8DyZRybqZ7JVXedBXXL
A R126-0507
ACUTE TOXICITY TO AQUATIC INVERTABRATES (DAPHNIA MAGNA)
TEST SUBSTANCE
Identity:
Perfluorooctanoic acid, ammonium salt; may also be referred to as PFOA ammonium salt, Ammonium perfluorooctanoate, PFO, FC-
116, FC-126, FC-169, or FC-143. (Octanoic acid, pentadecafiuoro-
, ammonium salt, CAS # 3825-26-1)
Remarks: The 3M product lot number was 37. The test sample is FC-143. It's purity was not sufficiently characterized, though current information
indicates it is a mixture of 96.5 - 100% test substance and 0 -- 3.5% Cs, C7,
and Co perfluoro analogue compounds.
The following is an abbreviated summary of several previously completed Daphnia magna studies conducted to evaluate diet. These studies have been superceded by a more recent test.
METHOD:
Method:
Not noted.
Type: Static acute
GLP: No
Year completed: 1982
Species: Daphnia magna
Supplier: USEPA-ERL, Duluth, MN.
Analytical monitoring: Temperature, pH and dissolved oxygen
Exposure period: 48-hours
Test organism age: First-Instar young (0.5-1.5 mm)
Pretreatment: Not noted.
Dilution water: Carbon-filtered well water.
Stock and test solution preparation: Test solutions were created by a
common stock solution, 5 g/L
Concentrations dosing rate: Once
Stabilityofthe test chemical solutions: Not noted
Exposure vessels: 250 mL glass beakers containing 200 mL test solution (6
cm depth).
Number of replicates: two
Number of daphnids per replicate: 10 -- 20
Number of concentrations: five plus a blank control
RESULTS
Element values: 48-hour ECs = 126 (86-183) mg/L to >1000 mg/L.
Element values based on nominal concentrations
03811
CONCLUSIONS `The test sample results for 48-hour ECso for Daphnia magna were inconsistent. Values generated in May of 1982 determined 48-hour ECsos to be >1000 mg/L. The value generate in June of 1982 indicated a 48-hour ECao of 126 mg/L with a 95% confidence Interval of 86 to 183 mg/L. These inconsistencies may be due to differences in diet. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Kiimisch ranking 3. The method is not described, sample purity `was not properly characterized and it lacks analytical confirmationoftest substance concentrations. The values from multiple tests contradict each other. The purpose of these studies was to evaluate different daphnid diets. REFERENCES These studies were conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, completed from May to June, 1982. OTHER Last changed: 5/25/00
03812
AQEUNAVTIIRCOMTEONXTIACLI.TY LADBAOTRATOSUREYET Tyre Tost 4B hy. JC go Material Tested fC 43
Lot_3
Tes: Organise LJ. "Magna Source, Oofuth RL Dare Rec'd |Wewhpatch No = .
Age: First-Instar young (0.5-1.5wm) No. Crganisms rer treatment _ QC
Test Chamber: 250ml glass beaker Test Solution Vol.: 200ml Test Solution Depth: Gem.
Time Stirted_l0i30+ [oro Date Started_/2g/Dagte Completed,SfsfEz
Dilution Weter_Carhon-filtered well water (Temp. 24 C, D.O. PPh, PH___)
tvestigater_ AW.(uelb
4G, (95% Confidence Limits).
(Full Signature)
ETR E E
Tora
TAou1r0s.0]||
Torar9"8F1 ours
0.|0
8
kZyaFrFfoupreso| . [eaHToubs.
o.|mg/1 Added || No. Dead| ph | ppm|| No.Dead {ph | ppm| No.Dead bit | ppm{INo. Dead bh fp;
ome ~ [10T[ lo larledl 0[| || o bis
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4
Effects other than Mortality
EC
Tro|
Total No. Attectod
No.|] Symptons or Reactions || 24-tr. 48 br.
72-Hr,
96-Hr.
emer] A]
rr
rr
[rr
rn rr
Ll
Comments:
a
c03813
AQEUANTVIICROTMOEXNITCAILTY LDAASTOARTOSRUYEET
Tyre Test_48ha. LCro
Material Tested fC /43 Lot, 31
Tes: Organism_O, wagua Source Dolidh ERL vate weed (grygpath vo.
wins soe ar gore ||presteyl a sani [ee] Total 0.0]| Totes vo Totar | po. [od] p Age:
Test
CFhiarmsbte-rI:nst2a5r0mlyougnlgass(0b.e5a-k1e.r5rm)TestNo.SolCurtgiaonnismVsol.r:er20t0rmelatTmeenstt
10
Solution
Depeh:
em.
Tine Started_g:308
Date Started /9/#2 Date Comnieted $76/82.
Dilution Weter_ Carhon-filtered
Investigater__Ag wells
well
water
(Temp.
LC,
(95%
C, D.0.____ppm, pH___)
Confidence Units)
(Full Signa- ture)
Kel 4 uy
Wed 4ny
nit,
otal
24 Hours
48 Hours
2y H-Hours
S&-Hour
No. | mg/1
Added No. Dead| pH| pps
No.Dead| pH | ppm | No.Dead pH| ppm|INo. Dead bH Jp
cfemeall Hoo[[190 lazleallos [ "12 4} Ll
[2 se lle
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or. A 7 dia
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coments: Stoeh sotulive Sa3ld Soluble al Zk co
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Effects other than ortality
ec,
No.
ell
Symptoms
or
Reactions
24-Hr,
48 Hr,
72-Hr.
J
r LIr BESTOOPY AVAILABLE|
SN |ES
| ESS | NSO
| ES
L_1
Commenes:
96-Hr,
3814
AQUEANVTIIRCONTMOEXNITACLI.TY LDAABTOARTSOVREYET
eyTT er i Tyre Tost 434 ICon
MaterialTested fC[F lot 37
Test Ocsanton Dawagyn Source LLBOL Dace Rec'd gygatch No.
Age: First-Instar young (0.5-1.5em) No. Crganisms rer treat26ment_
Test Chamber: 250ml glass beaker Test Solution Vol.: 200ml Test Solution Depth: bcm.
Time Started _J[:30 aM Date Started_glyjDfaftge Comlered_(/2/B2)
Dilution Weter_Carbon-filtered well water (Temp._24 C, D.0.___pom, pH)
(Full Signatur,
nit.
No.| mg/1
clema
[ S Wo TT 2 TTfo [| all -Total
Hours
48 Fours
Added|| No. Dead| pH | ppm|| No.Dead| pH | ppn'|
F 72 Hours
5
No.Dead ph | ppm |INo.Dead bh |
Tao 1TTToTT8 1] Te]
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. ve = [76/1/02 WI SS0fodin o=r = Siffre
CommentsE: E
_--_--
Effects other than Mortality
ECgq,
No. || Symptoms or Reactions 24-Hr. 48 Hr. JyFa-hr.
mLeaTr [a Jf]
ly IJl ttee ctd deeti5 on [|toc JIa5l|1]|
[1
Comments:
A96tir. cere
BEST COPY AVAILABLE
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InToERoAFTIONS IN<ATERCEPT SLOPE
7
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2.747308
HETERDGENIETY FACTOR (> = 1
LE-S0 =
589.9821
3555 PPCCTT.. ULPOPWEERR CCOONNFF.. LLIIPnIITT ==
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FITTED FRACTION 00..500356786759239 0.8987523
NIDTEDRFATIONS INAT>ERCEPT - SLOPE
CLHIINEASROITFYOR-"CHPIROESROEIFLOIRTY
&
-2.552151
1.788321
7.774071
0. 005300145
HSIENTCEEROSGEINSTETGYREAFTACETRORTHAGNi 1.= 7TH.E774C6O7NF1. LIMITS 5AR=EsNeOsTz.sL7E1FINED
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03816
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BEST COPY AVAILABLE
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1000
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HETERDBENIETY FACTOR (Hy = 5.479644
LC=S0 =
621.606
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7 a8 HR RUN A
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LOG
FRACTION
FITTED
i
.
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BCETTNCEERGREN1I3ETBYRERFRTOETRORTh(Ahy 10= 7TH.E774C0O7N1E. LIMITS 6 A=RE FGEEfgoITElFIND GO3817
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FRREASCPTOINODNING 00..13 1
BEST COPY AVAIL ABLE
FFRIATCTTEIDON 00.50039878676935 0.895751
oI_ToERATIONS INpTyERCEFT SLOFE
`
3.58215 1.78832
HSEITNECERDSGEN1ISETGYREAFTAECRTORTHAoNh) 1= T1H5E.5C4O8N0F%.
Le-si =
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3355 FPCCTT.. ULFOFWEERR CCOONMEE.. LLIIMNIDTT ==
LCHIINEASROITFYOR CFHRIOBRSEoILFIOTRY 15. 54805 8. 045137605 LINITS AR=E 3aNOnTz.sDaEsFINED
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03818
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::E pH S::
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sr i eoae ae TL NEsceneries
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tA {:rSloLssI not sianisicantiy L disrerfT enrt feron serne.esnrsernand;::
m So =I IissEsoEss
aImetercess
ER -- Ltomees aimiEnE
957 confidence Limits emvuesm. etsm is
Treorstical Saentaneous Ressense Rate = 0.0000
038519
BTRUIRMLMIENDGTOSNPERAERSMEAANR-CKHA.RBEIRNC.METHOD FOR ECS0 AND LCSO VALUES IN BIOASSAYS
CALCULATION
OF
FM.OAR. RHEAFMEIRLETNOCNE.,
CITE R.C.
RUSSO,
AND
TRIMMED SPEARMAN-KARBER METHOD
FRO.VR. ESTTHUIRMSATTOINN,G
M1E9D7I7.AN
LENEVTIHRAOLN.CONSCCIE.NTRTAETCHINOONLS. IN11(T7O)XIC7I1T4Y-71B9IOASSAYS.
CORRECTION 12(4) 417 (1978).
DATE TEST W,
s1 /31/82
CSPHEECMIIECSA.L
FDca-p1h4ni3a magna
RADWURADTAITOAN
38 hours)
CNUOMNBCEERNTREAXTPIOOSNE(Dmg/L) MORTALITIES
10.00 a0
100.00 a0
$00.00 a2
1000.00 a0
a
12
a2
40
SSFPEEAARRMMAANN--KKAARRBBEERR
TRIM ESTIMATES
eCs0. 10.00 125.7965775
95% 95%
ULPOFWEERR
CCOONNFFIIDDEENNCCEE
18863..2399
03820
--------------
BURLINGTONRESEARCH.INC.
Trimmed SECoSeOarmaandn-KLaCrSbOerValMueetshodin foBrioaCsaslacvuslation of
6.9076 3
x
3
33
6.044E
x
3
3
33
L
5.1808 3
N
3
c
3 3
x
0 N
a.517 3
c E
33
N T
3.48363
R a
3 3
T 1
3 3
N0
2.590 3
33 x
1.72683
33
33
-8634E
33
3
3DDDDLDeEtDoDDDDDDLeDzEsDDDDDDDDDDDLDeDsEoDDDDDDDDDDDDLDeE7sDDD DIDDDDDODDDE
Lc100
MORTALITIES
ee
Co
03821
FC-143 Daphnia magna acute static 46-hrs exoosure
PLOT OF ADJUSTED PROBITS AND PREDICTED REGRESSION LINE ProbTioev
o
5
or
or
eal"
sv
ot
a-r
elLL
EN
2
i
on~ Ecot
03822
cio cas cso c7s ecoo
Ecos
e
FC-143 Daphnia Estimated
Point
EC EC
51..0000
ECEC1105..0000
EECC5805..0000
ECEC9905..0000
EC99.00
magna acute static 48-hrs excosure EC Values and Confidence Limits
Conc. 153..80670872 2260..70862345 30970..77999412 461300..83734016 1406.5795
9L5%owCeornfidenceUpLoiemrits
C03823
