Document ZeDQgD1nDVxVmYqgXMqEBbep

M -0318 A C U T E T O XIC IT Y T O A Q U A T IC IN V E R T E B R A T E S (D A P H N IA M AG N A) T E S T S U B S T A N C E ___________________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which m ay also be referred to a s P F O S , FC-95, or a s a com ponent of FX-3104. (1Octanesulfonic acid) (C A S # 2795-39-3). R e m arks: The 3M production lot num ber w as not noted. T he test sam ple is F X -3 1 0 4 ,6 % . Current information indicates it is a mixture of 0 .4 8 % P F O S , 14 .09% diethylene glycol butyl ether, 5 9 % water, 1 7 .6 % ethylene glycol, 1.4 4 % sodium octyl sulfate, 4 % inhibitor, 2 .0 % polyoxyethylene monooctylphenyl ether, 1.2 5 % propane sultone foamer, 0 .0 3 % sodium lauryl sulfate, and 0 .1 % benzotriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fiuorochemicai component o f the test sample. M E T H O D :____________________________________________________________ M ethod: Not given. Type: Static acute GLP: No Y ear com pleted: 1985 Sp e c ie s: Daphniamagna Sup p lie r: In-house cultures. Originally obtained from U.S. E P A -E R L , Duluth, MN. A nalytical m onitoring: Temperature, pH, and dissolved oxygen. E x p o su re period: 48-hours T e st o rg a n ism age: <24 hours old Statistical m ethods: ECso valu e s calculated using logistic regression m ethods (probit analysis). T e st conditions: D ilution water: Carbon-filtered well water. D ilution w ater chem istry: Not given. Lig h tin g: Not given. S to c k and te st so lu tio n preparation: A primary stock solution w as prepared by dissolving 1000 mg of test substance in 500 m L water. Exp osure concentrations were prepared by addition of stock solution to each vessel. E x p o su re v e sse ls: 250 m L g la ss beakers containing 20 0 m L exposure -- solution at a depth of 6 cm. Num ber o f replicates: 2 N um ber o f d a p h n id s per replicate: 10 N um b er o f co n ce n tra tio n s: five plus a blank control W ater chem istry d u rin g the study: 002993 pH range (0-48 hours): 8.1 - 8.2 (control exposure) 7.7 - 8.1 (1000 mg/L exposure) Tem perature range (0-48 hours): 22 C D isso lve d oxygen range (0-48 hours): 8.9 - 9.3 mg/L (control exposure) 7.5 - 9.3 mg/L (1000 mg/L exposure) R E S U L T S _____________________________________________________ , N om inal conce ntrations: Blank control, 100,180, 320, 560, and 1000 mg/L. Elem ent valu e s: 48-hour E C 50 = >1000 mg/L Element values based on nominal concentrations^. R e m arks: Testing w as conducted on a mixture a s described in the Test Substance Rem arks field. T he values reported apply to that mixture and not the fluorochemical proportion alone. C O N C L U S IO N S ______________________________________________________ The test substance 48-hour E C 50 w as determined to be >1000 mg/L. Subm itter: 3M Com pany, Environm ental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 D A T A Q U A L IT Y _____________________________________ _________________ Reliability: KlimisCh ranking = 3. Study lacks description and records of method followed. The study lacks information on purity of the test substance and analytical confirmation of the amount of fluorochemical proportion in solution. R E F E R E N C E S _______________________________________________________ T his study w as conducted by the 3M Com pany, Environm ental Laboratory, St. Paul, MN, Lab Request number C 1 1 1 1 -2 ,1985. O T H E R ______________________________________________________________ L a st changed: 6/26/00. 002994 ENVIRONMENTAL LABORATORY AQUATIC TOXICITY DATA SHEET i* TEST T Y P E ^ fe ^ , 7 b t f i* C ^ S T * 7 7 f '. CHEMICAL TESTED L OT TEST ORGANISM ^ Z ^ ^ ^ y ^ SOURCE/ X g ^ ^ ^ ^ !---- DATE REC'D / / Z / AGE OF TEST ORGANISMS : < 2 4 h r s . o ld in sta rT T n e o n a t'e s) NO. ORGANISMS PER TRFATMFNT / O TEST VESSEL: 250ml Pyrex g la ss beaker TEST SOLUTION VOLUME: 200ml DE^H: 6cm TIME STARTFD DATE STARTED DATE r.OMPI ftfh DILUTION MATER ^ C a rb o n -filte re d j< e ll water (Temp.^ < L 2 C , D.O. ppm. pH / 'f, / 1 " A N A L ^ ^ ^ ^ ^ ^ ^ d ^ ^ ^ C bQ (9 5 % Confidence Lim its ) ^ > M O O TEST Initial Total VESSEL Cone. Amount NO. rag/l Added X jfinJ Oontro! 24 Hours Total No. pH Dead n 48 Hours D.O. Total No. pH ppm Dead ' n D.O. Pi H w?, loo IW \Ci.. IPl r> .n HI yn q , j ^20 -T rrjQ fiff loo n o n r % G Jo 7,f> 3 , 11 %* Test Teno. K 1 '? ? CY ? Initials & Date <rz-- ? s X ^ /7 r/g T COMMENTS : J/<yA~r 2 2 '^ SECONDARY EFFE F k sri VESSEL Nn Syiqptans o r R e a c tio n s C o n tro l * T0T2 U T ER EFw . ' i 002995 ENVIRONMENTAL LABORATORY AQUATIC TOXICITY DATA SHEET TEST T Y P E T & a r iL S r x r j r .c h e m ic a l t e s t e d TEST ORGANISM l ot_________ -- DATE REC'ILJ 2 / & L _______ AGE OF TEST ORGANISMS : < 2 4 hrs. old in s t a rs (neonat/s) NO. ORGANISMS PER TPFATmfnt / O TEST VESSEL: 250ml Pyrex g la ss beaker TEST SOLUTION VOLUME: 200ml DEPTH': 6cm TIME STARTFD DATE STARTEn A COMPLETED, M / x . s: DILUTION MATER C^ ^ o n - f ilt e r e d wen water (Temp. 1 2 ^ C, D.O. ^ ppm, pH f i r/ ) ANALYST<S ^ ^ 2 ^ ^ ^ < ^ ^ W ^ E C . . ,, (95% Confidence Li mi ts 2 2/ 2 / . VETESNSSOET.LIrCnaoignt/c1i.al Total Amount Added -*<J HT Contro! 24 Hours Total No. pH Dead o 48 Hours D^.pOrn. Total No. Dead pH * oV D.O. pgn F?-9 "1 Ino ' lo cV i r / f*/ijji IflT) IP o r HI z-*> xzn, r> n HjOf t l 2.-4 ,w > ^C/> o ?-;T toon too o on n &Test Temp. C Initiais Date g g ^M ssr. COMMENTS :J^pt ........ -r - ----oe---i-/ /ptVO*Xjt/l O O A < f . - - -- -- -^---- --- ----- m /tq / tf- ' ( /t( Trff* 002996 ENVIRONMENTAL LABORATORY T A B L E I. S U M M A R Y OF ACUTE T O XICITY OF / , <Y,, { o h tS A M r ) TO D A P H N I A M A G N A U N D E R, ___________ EX P O S U R E C O N D I T I O N S * 1 ^ CHEMICA(mL gC/O1N)C E N T R A T I ON CONTROL mo [SC ___ __ ..SImQ----no a __ 2A-HR. 0 o c J o o PERCENT MORTALITY 1 A8-HR. 0 c c c 0 o (EC__ 95Z C O N F I D E N C E L I M I T S ) * 2 ^ 50 *g/l > sooo > /000 (1) Data w e r e averages of two replications for each concentration with ten (10) daphnlds per O replicate. A total of twenty daphnlds were used per concentration. o\ N (2) E C j p V a l u e s were calculated using logistic regression methods (Problt Analysis). CD