Document ZVjkgdGeoq30wx5bXj5D2gq8
BIODEGRADATION (301E)
TEST SUBSTANCE
Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredto as PFOS, FC-95, or as a component ofFC-203. (IOctanesulfoniaccid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber was notnoted. The testsample isFC-203. Currentinformatioinndicatesitisa mixtureof 1.34% PFOS, 35% diethyleneglycolbutylether,37.85% water,20% ethyleneglycol,2.66 % Sultonefoamer, 3% sodium octylsulfate0,.1% sodium laurylsulfatea,nd 0.05% tolyltriazole.
The followingsummary applies to a mixture with incompletely characterized concentrations of impurities.Data may not accurately reflectdegradation potentialof the fluorochemical component of the testsample.
METHOD
Method: ModifiedOECD Screening Test,OECD 301 E, withDOC Analysis,
1981 version.
Test type: Ready Biodegradability
GLP: No
Year Completed: 1982
Analyticalmonitoring: Dissolvedorganiccarbon (DOC)
Statisticamlethods: Resultswere determinedby calculatioonfthe % DOC
removal and graphicinterpretation.
Test organism source: A 50:50 mix ofsoilextractand secondary effluent.
The secondary effluenwtas the supernatantfrom an activatedsludge
aerationbasinatthe Metro Wastewater Treatment Plant,St.Paul,MN, while
the soilwas from the CityofWhite Bear Lake, Ramsey County, MN.
Test condition:
Dilutionwater: Deionizedwater
Mineral NutrientMedium: Nutrientmedium per OECD 301E method
(1981).InitipaHl 7.0.
Reference and testsolutionpreparation:The testmaterialwas
prepared by dissolving153 mg intwo literosf mineralnutrienmtedium.
This solutiongivesa finaltestconcentratioonf 20 mg DOC/L. The
referencesubstance,sodium benzoate,was prepared by dissolving
30.3 mg inone liteorfmineralnutrienmtedium. This solutiongivesa
finaltestconcentrationof 20 mg DOC/L.
Test vessels: Not given.
Incubation conditions:Constant dark conditions
Temperature:
23.5- 240C
Agitation:
Continuously
Number ofconcentrations1:plussodiumbenzoate(reference substance)and blank,allinduplicate. lnoculum conditionon testinitiationN:ot given . Element Basis: Decrease indissolvedorganiccarbon compared to the blanks. Test substance flaskconditions:Not given.
RESULTS
Nominal concentrations: Blank control,sodium benzoate at-20 mg DOC/L and at-20 mg DOC/L plusHgCL2 (inhibitedt)e,stmaterialat-20 mg DOC/L and at-20 mg DOC/L plusHgC12,allinduplicate.
Element values: 27-day Degradation= 76.7% duplicate1 and 81.3% duplicate2. Mean value = 79%
Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The values reportedapplytothatmixtureand not the fluorochemicalproportionalone.
CONCLUSIONS
The FC-203 % degradationbased on the mean DOC removal value was 79 after27 days.
Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133
DATA QUALITY
ReliabilitKyl:imischranking= 2. This s-tudymeets the criterifaorquality testing.However, the sample puritywas notproperlycharacterizedand the study lacksanalyticaclonfirmatioonfthe amount offluorochemicalproportion inthe solution.
REFERENCES
The studieswere conducted by the3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number 8483, 1982.
OTHER
Last changed: 6/27/00
interCnoarlrespondence
.cc: vD. L. Bacon/R.L. Bohon - 21-2W-05
VR. Gritzky - 3M Neuss VD. R. Ricker 236-2B-01 VJ. A. Pignato 2362A-01
"To: K. F. SCHROEDER - 3M NEUSS, GERMANY
From:E. A. REINER - ENVIRONMENTAL LABORATORY/EE
SubjectB:IODEGRADATION TESTS Date:APRIL 21, 1983
& PC
21-2W-5
3M
.Envir. Assess..,Inq. #823,26,.,3M Neuss, Germany FC-203, FC-600
Attached is a copy of our report on the modified OECD screening tests on FC-600 and FC-203. While the results of this testing does not precisely repeat those which we obtained on FC-203 in 1980, they do confirm that FC-203 is readily biodegradable according to the test method criteria. The present results also show that FC-600 is readily biodegradable.
The reason that these results do not precisely match those of our previous testing is not known, but biodegradation test results are characteristically somewhat variable. This variability is often explained by one or more of the following three factors:
1. Differences in the bacterial species or strains present in the inocula of tests started at different times.
2. Variations in the formulation of a product from one lot to another.
3. The presence of inhibitory materials such as possibly toxic byproducts or inadvertent contaminants.
K. F. Schroeder Page 2 April 21, 1983
The percent biodegradation results given in the attached report are equally app@icable to both the concentrated AP'FF products, as sold, and the diluted products, as used. Dilution with pure water would have no effect. The reason for this is that testing is actually done on a very dilute portion of the concentrate (20 ppm as TOC). llot-.tIt,i(,ciotic(-.@ntrciotre lli(@3 or 61 tisago Iov(.,lwoul.(]1)(-' diluted to this same concentration for testing. The dilution factor is not considered when calculating the results.
I have reviewed the table of biodegradation data that you sent us. Some of the variability in results which you show is not unexpected. This is particularly the case with the 5-day BOD test results. Biodegradation of AFFF products is usually only partially completed within the first five days of a BOD test and.the percent of biodegradation that has been completed in this time period is highly dependent upon both the viability of the microbial inoculum and the fraction of the organisms in the inoculum which are capable of degrading the organic compounds.
Results of 20-day BOD tests are less variable because biodegradation is usually more nearly complete and proportion of organisms capable of degrading AFFF products has increased through growth. But, 20-day BOD tests are susceptible to another type of problem, "nitrification." In these longer tests, populati-ons of bacteria can develop which can oxidize the ammonia,in the BOD dilution water making it appear that the test material is more biodegradable than it actually is. In the Environmental Laboratory we are now taking precautions to avoid this problem by using "nitrification inhibitors," chemicals which inhibit,the oxidiation of ammonia.
A point of confusion in your table of BOD data is the reporting of different BOD5 to COD ratios for both the undiluted and diluted AFFF products. This doesn't make sense because BOD and COD would decrease proportionally with dilution with the ratio remaining the same. These differences which you indicated were reported in Hellmann's work must therefore be due to variability between one ROD test run and another and not to dilution.
K. F. Schroeder Page 3 April 21, 1983
We hope the attached OECD test results and this
explanation of the biodegradation data in the table which
you provided are helpful to you in dealing with potential
customers concerned about biodegradation.
If you or your
customers have any further questions about biodegradation
test restilts on 3M AFFF products, feel free to contact
li@;. Wo'll. do otir best: in trying to get a satisfactory
answer for you.and your customers.
For our own benefit and at our expense, we are in the process of repeating the OECD screening tests on the FC-600 and FC-203 samples used in this testing. We are interested in seeing how repeatable our testing results are when the same samples are used in two separate tests. We will inform you of the results of this test.
EAR/mb Attachment:
Report on the Biodegradation FC-203 by the OECD Screening
of FC-600 and Test
Form 6747-11-E
TECHNICAL REPORT SUMMARY
TO: TECHNICAL COMMUNICATIONS CENTER - 201-2C-12
(Important-Ifreporitsprinteodn bothsideosfpaper,sendtwo copiesto TCCJ Guidelinesorneversseide.
Division
3M Environmental Laboratory (EE & PC) St. Paul Minnesota
3M Germany
Roport Title
Biodegradation Testing on FC-600 and FC-203
To
K. Fe SchrQeder, 3M Neuss, Germany
Au thor(s)
E. A.@keliner, Environmental Laboratory - 21-2W-05
Notebook Reference
None - See 3M Environmental Lab Request 8483
Dept Number
0535
Project Numbor
9972410100
We-po-r-t-wu-mso-r
C()I
ForladCoveredor Date
4/18/83
Employee Number(s)
47816
No. of PagesincludingCoversheat
15
SECURITY 10. KEYWORDS: Lab Code
OLI)erKeywords
[]Open
Report& Summary 0 Closed Re'port-ODen Summary
CURRENT
OBJECTIVE:
3M CHEMICAI. 1 REGISTRY-
b, [3 Check box ifnow chemicals are reported.Use Chemi- Xcal Registry Form 6092 to report all new substances,
1. To measure
the biodegradability
of two "Light
Water"
products using the internation@klly recognized Modified
OECD Screening Test.
2. To compare results with those previously obtained using the same test method.
REPORT ABSTRACT: This abstractinformationisdistributebdy theTechnicalCommunications Centertoalert3M'ersto Company R&D.
This study used the Modified OECD Screening Test with dissolved organic carbon (DOC) analysis to measure the biodegradability of "Light Water" Brand AFFF products; FC-203 and FC-600. In 28 days, the DOC levels of FC-60&, degraded by 80% and 92% for an average of 86%@'in two parallel'tests. In 27 days the DOC of FC-203 degraded-by 77% and 81% for an average of 79%. The FC-203 result differed from a similar test done in 1980 in which 93% DOC degradation occurred within 21 days. In the present study parallel sterile controls proved that the DOC loss was not du e to chemical-or physical procetses such as adsorption, vo'latilization, or precipitation of the organic compounds.
InformationLiais Initials:
FC-600/FC-203 P,cg-ie 2 April 18, 1983
Introduction
The 3M Environmental Laboratory undertook this study of the biodegradability of "Light Water" products, FC-600 and PC-203, I-)(-@cause of concern expressed by 3M Germany about inconsistencies in 1),ISL biodegradation test results.
Modified OECD Screening Tests conducted in 1980 by the 3M Environmentzkl,L.@4I)oratoryshowed that three "Light Water" products were highly biodegradable. FC-203A, FC-206, and FC-3017 (FC-206 P) all were shown to have 90% of their dissolved organic carbon removed in the OECD Screening Test. These results, however, contradict those of .H. Hellmann and D. Muller(l) usin a similar yet not identical test method, the TOC Degradation Test( ). In their study FC-206 and FC-3017 were found to have total organic carbon reductions of only 6% and 8%, respectively, in 21 days. These low biodegradation results of Hellmann, however, were also contradicted by his own work. iiis BOD5/COD ratios for FC-3017 and FC-206 were 0.5 and 0.4, respectively. This showed that 50% and 40% of the theoretical oxygen demand was expressed within only 5 days. These levels of biodegradation are much greater than the 8% and 6% TOC removals which he observed in the even longer (21 day) TOC degradation test. This is clearly an inexplicable discrepa,ncy in his work.
Hellmann's TOC degradation results are also contradicted by BOD20/COD measurements made by the 3M Environmental Laboratory and other independent laboratories on 3M AFFF products. Such results typically show BOD20 values to range between 60 and 190% of the COD. For example, a collection of such data on 3 U.S. military specification AFFF products made by 3M is shown in table 1.
Pag@ 3
TABLE I
BOD, CARDONACEOUS BOD, AND COD DATA ON MIL-SPEC Ai,'Ft-i'-litODUC-1-
BOD20
C-BOD
COD
BOD20
C-BOD20
Lab
(mg/kg) (mg/kq
(mg/kg) -TO-D
COD
FC-203C Lot 501
3M
scolooo
600,000
730,000
.79
.81
740rOOO
Pace
.8OOtOOO*
7301000*, 810,000
1
730rOOO
Serco
440,000
440,000
737,000
.60
.60
Ca sule
5701,000 620,000
950rOOO
.6
.65
Ga@braith
616,000
67loOOO
776,450
.79
.86
S & W Seed
Galbraith
670,000
641,000
776,450
.06
.02
Knoxville
Average S. D.
575,000
594,000
782,000
.73
.75
85,000
90,000
Borooo
.12
.12
Calculated BOD's from different dilutions were very inconsistent. Pace agreed to repeat this work but inadvertently lost their repeated test results as well. We did not recjuest a third testing nor did we include these
values in the averages.
FC-206C Lot 502
3M
Pace Serco Capsule Galbraith S & W Seed Galbraith Knoxville
290,000
350,000 240,000 260,000 416,500
395,500
290,000
370,000 260,000 320'000 411:000
374,000
380,000 360rOOO 450,000 370,000 410,000 407,250
407,250
0.78
0.78 0.65 0.63 1.02
.97
0.78
0.82 0.70 0'.78 1.01
.92
Average So Do
3M Pace Serco Capsule
325,000
338,000
396,000
.81
.83
73,000
57rOO
33rOOO
.16
.11
FC-780B Lot 501
240,000
230,000
320,000
.74
.71
330,000
250,000
250,000
350,000
.71
.71
210,000
220,000
287,000
.73
.77
240,000
230,000
360,000
.67
.64
Average so Do
235,000
232,000
329,000
.71
.71
17,000
13,000
28rOOO
.03
.05
FC-600/FC-203 Page 4 April 18, 1983
Methods and Materials
1. Procedure
Testing was perforin(-,@i(ni accur(lanc(--witli tlio01!,'CD testing of chemicals adopted May 12, 1981.
I.or
2. Chemicals
The reference material used was laboratory grade sodium benzoate (C7H5NaO2) F.W. = 144.11. A 30.3 mg sample of this compound was diluted to 1.0 liters with the nutrient solution described by the method to yield A theoretical DOC of 20 mg/l. The "Light water" samples used were supplied by G. Piessens, E.T.S.L. and consisted of FC-203 and FC-600. Lot numbers were not provided. Nutrient solutions of the "I.ight Wc-iter"products with DOC's of approximately 20 mg/l (based on calculations from the formulation) were prepared by bringing both 527 mg of FC-600 and 153 mg of FC-203 to 2.0 liters with nutrient solution.
3. Inoculum
The inoculum was a 50/50 mixture of the soil and secondary effluent inocula each prepared in accordance with the OECD procedure. The soil used was fresh loamy sand garden soil obtained from White Bear Lake, Minnesota, and the water for the soil extraction was chlorine-free well, water. The secondary effluent sample was the supernatant from an activated slud(c aeration basin at the St. Paul Metro Wastewater Treatment Plant. The combined inoculum was passed through WhatmanD 54,filter paper
prior to usage.
4. Sterile Controls
Replicate vessels containing 400 mg/l HgCl2 served as sterile controls for both test materials and the reference compound. The sterile controls were handled in the same-way as the viable cultures.
5. Instrument
The organic carbon analyzer used was a Dohrmann DC-52A. Its sensitivity limit is approximately 2 mg of carbon per liter.
6. Sample Handling and Storage
The sample preservation method used was that prescribed by the TOC manufacturer. The method involved adding 1 drop of concentrated HC1 per 10 ml of filtered sample. This brought the samples to less than pH 2. The samples were then stored under refrigeration in vials with aluminum foil-lined-caps. Dohrmann literature indicates that the method. can stabilize potassium hydrogen phthalater a readily biodegradable material, for several months.
FC-600/FC-203 lllito5o April 18, 1983
Due to problems with the sensitivity of the TOC analyzer, onl.y half of the samples were analyzed within a month after sampli.ng. Following 4-5 months of storage, all of the samples were reanalyzed. Comparison of the TOC analyses done within I inoiii-.1-i with those done after 4-5 months of storage showed that in only one stored sample had the TOC changed significantly. This sample was discardea.
Results and.Discussion
Table 2 summarizes the resul*tsof this study. Appendices I and 2 contain the actual data sheets and plots of the degradation as a function of time. Table 2 shows that the sodium benzoate reference compound was nearly completely degraded within 14 days. Degradation of both FC-600 and FC-203 continued throughout the course of the 28-day test period without plateauing. At 28 days the average TOC removal for FC-600 was 86% and at 27 days the average TOC removal for FC-203 was 79%. The test procedure recommends that chemicals giving a result greater than 70% loss of DOC (within-28 days) should be regarded as readily biodegradable. These 3M formulated.AFFF products are thus even more completely degraded than is necessary for a chemical to be considered readily biodegradable.
TABLE 2
Biodegradation (% DOC removal) of Sodium Benzoate, FC-203 and FC-206
Product
% Degradation at Day:
7
14
21
27
28
Sodium Benzoate Sodium Benzoate Duplicate
94.4 92.3
94.7 96.3
93.2 99.6
93.5 97.7
94.5 100.0
FC-203 FC-203 Duplicate
-.37.4 43.0
59.2 54.6
68.4 66.0
76.7 81.3
78.0
FC-600 FC-600 Duplicate
46 47.2
63.5 67.1
73.5 80.2
82.1 88.7
79.9 92.1
*Sample discarded due to probable contamination.
The sterile control data in Table 3 provide further substantial evidence that soluble TOC loss from the test samples is not due to physical modes such as adsorption, volatilization, or precipitation. These samples were handled identically to the test vessels, except that they contained HgC12 to prevent microbial growth. At the end of 28-day test period, these controls contained all,of the initial DOC.
FC-600/FC-203 Page 6 April 18, 1983
Conclusion
TABLE 3
Percent
InitiaI DOC in Sterile Controls at 28 days
Product
DOC Remaining
FC-2U3
98.1
L-C-600
101.3
The present study demonstrates that Light Water products FC-203 and FC-600 are "readily biodegradable" according to the recommended 70% DOC removal criteria of the Modified OECD Screening Test. FC-203 and FC-206 had average losses of DOC of 79% and 86t, respectively.
References
1. Dr. H. Hellmann and D. Muller, "Environmental Impact of Fire Extinguishing Foam Compounds (Umweltgefahrdung durch
Feuerlosch-Schaummittell)." The study was carried out by the West
German Institute of Hydrology (Bundesanstalt fur Gewasserkunde), April 18, 1979.
2. D. Muller and T. Tittizer, "The TOC Degradation Test - A Process to Test Organic Materials for Complete.Biodegradation (Der TOC Abbautest - ein Verfahren zur Prufung von Oroanbischen Substanzen auf Vollstandiaen gi@'-ologischeAnbbau)." Developed at the Bundesanstalt for Gewasserkunde, Koblenz, Z. F. Waser-und Abwasser-Forschung (1979).
3. Organization for Economic Cooperation and Development, Guidelines
For Testing of Chemicals, Section 301 E, Ready Biodegradability: Modified OECD Screening Test, May 12, 1981.
4. Dohrman DC-,52A Operating Manual, 4th Ed., 1978.
..Page,.7,
Appendix 1
A N t4
SAWLE FORM SHEET FOlt IHE MODIRIEC) QECD SCREENI-NG TEST
4tISq 13 E:p. no.:
0 to OT St*Tt OT tOSt:
10 - ;@-7 - lr2-
4vm * standermaotorist,
Yheorptietl
vane.-:
2./7@
/I
lnocuium. Clip(.4)tiblh
t;arom siteayoor
rie %'IW
At Cgntrgls;
I TOC* I ooc*e I
*took solution or the to*t
rial
/1, dilution -
I
I
Ia al of nutrient solution) I
I
83 Carbon taterainations:
3C)p us Irisoul maiyees Culture asol no.
I (A M
f@,@,
ivnear.i pvc - concentrationsorter x aayii
leene. I
mo/I
I
loo/i 10 (Co)l 71 141 -211 271 28 1
Mineral nutrient
solutioniM
III
test sato-rT&-T
0" Nith
gnocu=us
42 al 021
I
lei - ---7-1
(Ct)
2'.
1
1-
I I ?I y /,-.-rI I
I
b2
bl b21
1
102 - ---I- I
l(Ct)
Irn-s-r-anluttionti
solution wittmutigiankl
test seterzal 1
out 1uh
02
al + 02
(ebi)
Y l,7 12,3
d) 12, o
I
I j,0
10,*71,:D@-i-l,iD.1 11
-1
osirl 0
note musli@ent
-61
im
Blank
al (Cbl)
L 1 10--710@,S@-3@0-71t
0 "SCOGNmt betws*^ON "W TOC valuesPoints towardsInsufficientsolubility *1 tu test uterial.
BW vg&Wo det@ifted otter **Mwww filtraties.
,AUEOAC,E OF 13LAtJi,-S*
AfTeR X pAys
7
iq
2 /.ot TO-1 03.r o,
LP,o
a g e /-E
fe 12
*Ready Hiodograda6ility:
ModL?Iod OECD Scroon*ing Test*
C; cya:uatlgo of Lew date;
FIO*kl
no.
DOC - concontrations minus
I 101
5 otx removal arter x
71 14 211 27, o2y8'
sit O)t ico lyq
*3t
1 93 I
03 J
01 02
2 for day x
'i 'I
ige
"Ready Uiodegradability: modified OECD Screening Test'
301 E
I Page 11
S. A N N I X
SAWLE roitm SIICET FOil Itif MOOIFICU OECD
Exp. no.:
-94'93
Date of start of test: 1,-1-817-
UP6 * Stendero coromrsais Thoorpli&!!t tI.;*.canc.: .
,l;naoculu:: C@,Elt-14RASX
roon iia4yvor:
7
V,.3 L 2!2 A
SCREENIW
.1f@!
/wi
TESY
As ganlra":
I TOCS I DDC*E I
latook soluttai;of the Lost material I
(1000 a 1, dilution
el of nutrient ig"tion) I
I
Carboi
U:
Culture
Td2r JFA*TEAIAL. i
fiesiti I Analyst* I sodium no.
Fc-- iTheor.1 ouc - concentrations ertc-r--x--aa-ysl
loonc. 1
09/1
1
NVI 10 (Co)l-&71 *vl4lAP2llop271 28 1
IlUneral nutrient,,
al
solutionXUII I I I
material
1
82
lww With
-f I
I
1.@
I
IIq II,, q'i 11 1',gI'',-7 '
61 + 821
1
1
1
1
1
1
1
I
(Ct)
7,i!i
1 2 2 11 / 1I.2 i.o 'I113.9'1 I q. r I'?. 1I4. q41 'II/!Sr..y-l0 1I
I
(Ct) I
bl b21
102 -
(fL,
autrilenti
solutioVnitboutilnkl
PIJ
ltest material 1
02
lout im
I;$ a I
linecu-1us
I
(cbI)
mineralokutft"t "lutten ut sionill
testwittae"lf butKUK
at
I (Cbl)
I
0 D$oosroononbtetweenM &PATIC v@4v*opointstowardsSawrtiesentsolubility of thetest"terist. P41 ut valvesdotu"ood aftermamwom rsittattea.
WtJ ATf..?ACr fO t 13LA (,KJ PA-RA
-:>a,ge
E
/.,a12
*Ready siodograda6ility:
j,
ModLflod OECD SersonLng Test"
C:eva:uatlgofg-rav dots,
low - concentrations minus
blw*o
Ina. 1
I 2
&San
mi@ - O)t
al - al
21 - 03t 102- D)
01 02
2
for,day x
2-03
Ia U-N removal afte
days I
1 71 141 211 r27 11, 28 1
1
I
100 P7.q I I?I
I lf%O I
lq3.
lqo157 1
me
A
!RF-SUL-TS
eelolt -To E)or-
A(( -T-0 Stlo") SAPAELE
(-a#j-rAvtlljA.T,
UALt4r.
ONE V gr Sol
)Page
"Ready Uiodegradability: Modified OECD Screening Testa
- ---------
301 E I Page 11
ANNi X
SAWLE f(MiN$MEET FOR ItifMQUIFICO9CC SC"EFNIUGTgLy
Exp. no.: I-A 4 1?tiq 3 Date of sre-Ttor test:
4ooh- 1 Standard notortal i
Thearvil@!!!
uonc.-
ianoculu::
roan slasy0or;--Dc--;L.,L
igrA A Ai Af)
As iggtrolg:
I TOC* I DOC** I
tOOk *Oluttan or the test materls 'low mg/l, dilution
...IIDCIO Al at-p utrient soigtion)-t-
I
81 Carbon daterainatilins:
MA-rC$2slL Floomi oina&yses cu-iture S*dLum ,no. I
Ec- 00 lThear.1 06 - Concentrationsofter-x dayal
leonc. 1
9/1
1
40/1 10 (Cc)leaf7t1^^14 o*21W 271 28 1
KtnerGl nutrlsnt ti n wlth
,t,a:Itl"ia*te-rraT
end lith
I
infoulu-N
I
42
lI -
I
I &I l@02 I
2 S,.g:j-qf4
II -"Y,s-iL30.1
II
I
11/3. 1 q, cy
0 I
i(Ct)
12
b2
bi b2 102 0
NtneraVnuttlent solutionwithoulibinkl
test motor-Lfil--i
02
but xm
I;# a
2. 3.1
I(Cbl)
testastefio4ut4ol@&nkt
(chi)
0 O0lff#,r,0t0e"nstt bftvolft DOC S"d TOC VGIUGS P*Lnt& tOW&VdB lftsufficienstolublilty
0*
vo4we detw"mw after embeeng riltrotloi%.
age '12t.
E
12
mo;Roaot siodo radability: Iris OCCO lcre*nlng Test*
C: L"tlon IOOC slow$
of rewim": IFI*Skl
no.
2 seen
Olt - 23t al - 93
821 - 03t 1.2 . m3
L ol 02
for day x
5 oac removal a 71 141 21F r2717 2YO 1
I "f"o
iq7.;i
177lrl1?(1*
Page 13 1 If i
100% i-44-4
9ax
I iT 1!
70% Lli
at
60%
50X@
40% ca
30%- r7
20X
Appendix 2
3M Envlromental Laboratory OECD Modified Screening Test with DOC Analysis Analyst: Wade A. Schell. Date of Start of Test: /0-27-t2La Request No.:
Test Material: Smj>iupq W-Pleo*rrs
21
27
NUMBER OF DAYS INCUBATED
L-@=
7
Page 14
I
I
3M Enviromantal Laboratory OECD Modified Screening Test with DOC Analysis Analyst: Wade A. Schell Data of Start of Test: 10-:Z7-12-
Lab Request No.: 13qS3
Test Material: FC-- 10
70%-
LU
60%--S.
5D%.
40%-Im LU
30%-
20X
10%
XV z
z
7. : ! I I ----------
21
2
NUMBER OF DAYS INCUBATED
Page 15
I
90%
80%.
70% LLS c3
CF/o 40% 3='7 20X
L.0 20
09
3M Envi.a ntal Laboratory
OECD Modified Screening Test with DOC Analysis
7-
Analyst: Wade A. Schell
Date of Start of Test:
Lob Request No.: -Sqw*3
Test tbt*rial:-F -(POO
-Awr
T
21
2@
NUMBER OF DAYS INCUBATED