Document ZLrYG8dE6oywJaV3nMObyYaY

: AR 226 0817 TOXICITY TO AQUATIC PLANTS TEST SUBSTANCE Identity: A mixture containing perfluorooctanesulfonate, which may also be. referred to as PFOS, FC-95, or as a component of FC-206CE. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: _ The 3M production lot number was not given. The test sample is FC-206CE (5/23/91). It's purity was not sufficiently characterized, though current information indicates itis a mixture of 0.92% PFOS, 15% diethylene glycol butyl ether, 76.09% water, 4% urea, 1.28% Sultone foamer, 1.55% sodium octyl sulfate, 1.05% polyoxyethylene monooctyiphenyl ether, 0.06% sodium lauryl sulfate, and 0.05% tolytriazole... cThhaerafcotlelorwiiznegd csounmcmeantrryataippolnisoesfitompaurmiitxiteusr.e Dwaittha minacyomnpoltetaeclcyurately reflect the toxicityof the fluorochemical componentof the test sample. METHOD: Method: OECD 201 and EPA-600/9-78-18 Type: Acute Static GLP: No Yearstudy performed: 1991 Species: Selenastrum capricomutum Source: Originally obtained from the Culture Collection at the University of Texas at Austin, maintained in culture medium at EnviroSystems, Hampton, NH. Element basis: Algal cell counts (cells/ml). Exposure period: 96 hours Statistical Methods: Performed using standard statistical techniques (Stephan, 1983). ECso values were calculated using the binomial or moving average method. The NOEC was calculated using Dunnett's test. Analytical monitoring: pH and temperature. Test Conditions: Algal NutrientMedium: Nutrient medium per U.S. EPA, 1978 prepared in deionized water. This nutrient medium provided all mineral nutrients essential for algal growth and also served as the diluent for all algal operations. The PH of this synthetic algal medium was ~8.0. Stock and test solution preparation: A 1000 mg/L primary stock solution was prepared in algal medium. Aliquots were added directly to algal medium in test vessels to create test solutions. 001802 Exposure vessels: 250 mL Erlenmeyers containing 100 mL test solution and capped with inverted glass beakers. Agitation: Continuous rotary shaker, rate of rotation not given. Number of replicates: 3 NInuitmiablecrelolflcooandicnegn:tra1t.i0oxns1:0* fcievlelsp/lmuLs. a blank control lLiigghhtst.ing: Continuous lighting at 50 uEs'm? using cool-white fluorescent Water Chemistry: PH range (0-96 hours): 8.29.9 (control exposure) 7.98.3 (1000 mg/L exposure) Test temperature range (0-96 hours): 23.0 - 23.4 C (incubator) _ RESULTS Nominal concentrations: Bk control, 150, 250, 400, 600, and 1000 mg/L Algal Growth Response at 96 Hours Nominal Conc., | Avg. Cell Count| Avg. Specific mg/L (cells/mL) Growth Rate Bk. Control 479000 | 0040 [0| 718000 | 0045 [250 |"415000 [T0038 12 | 2| 640000 | 240 2001 0| 00 000 33 0| 11070 (1) Av0er0age gr|ow0 th rate in control|compared0t.o0t0es0t concentra[tion1gro0wth0r|ate. Element values: 96-hour ECs: 96-hour NOEC: 456 (400-600) mg/L. 400 mg/L Element values are based on nominal concentrations. Reversibilityof Growth Inhibition: Not determined. Remarks: Testing was conducted on a mixture as described in the Test tShuebsftluaonrcoechReemmicaarlkpsrfoipeolrd.tiTonhealvoanleu.es reported apply to that mixture and not 001803 CONCLUSIONS FC-208CE exhibits a 96-hour ECs)of 456 mg/L with a 95% Confidence Intervalof 400 to 600 mg/L. The 96-hour No Observed Effect Concentration (NOEC) was 400 mg/L. `Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking 2. This study meets the criteria for quality testing. However, the study lacks information on purityofthe test substance aprnodpotrhteiosntuidnytlhaecskosluatniaolny.tical confirmation of the amountoffluorochemical ~ REFERENCES Test was conducted by EnviroSystems Division of Resource Analysts Incorporated, Hampton, NH at the requestofthe 3M Company, St. Paul, MN, Lab Request number J1884-2, 1991. OTHER Last changed: 6/26/00 _------ 001804 BEST COPY AVAILABLE tsoeaaschisee:usSSteuTdlayseTtniiittltyee.ofCpree-iscookcrrten ~ ; re Sfobectt1.eou]rt } 4i i%1 4 i F< iEga: y su, 1m a moa a \ EA 5 CSoAS E sBEE LC SP nReirtoresinsgLabsoraitorsy i Gi. LR ERoEE RE E ARe R o 1 onice anacapeetias TaeersrateSd)HLAoE R aa ; SEglni] B ha bR o es vi TT . GEgELT Ge NE GE -ai ; e fat es aae bcanLn maeoEEs oa Sis 0) Er Ea -* : ; ) OUTS 1. 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I. CES BEST COPY AVAILABLE 1. ro ce orey seron: es a 2 flofce omens , III. Indexof Tables A von 0 mere . . s s ". -- tino: aes uty ee fen siete Tot e So nms l ehal ee en nae E SR aYa aC e laE Ta TY : LEA Rr - -} Sr ) k 001507 BEST COPY AVAILABLE 111. pom or mans + | nae oll cCa hoemaidee'nattdTuarslcotneerdtuwtaitoenr owfad3 c55omprmetpaarteive 6 Table' 3. PeerceCnt crhanhge fTroom coTntnroltoratndettayvereaagte 10 Tole d. mHaatitn ettlacytinTe tconcntestions (58) n } Oe L. Teeratars sass dion tontey tot 1 F010 0.3. 58 sessured durtog toxicity test ` BT LAER Fn 1 24 a Gey EERE as GL nda SE RmieNad BLdfeoSn 0 daNp i g hReyesh n e | Ss Ba TT CeREE AcTOER ad EE RS E SDROa wea cla IS id Eo eo hay CR WE HEL ae feintesco airs . 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Growth data trom testy BEST tant copy AVAILABLE _--_-- Chal BREE cNnocmuiantarlaion [OMuTsber ooff CCoellllesppoerrMMiullitlieterxx1100 G(ohowur)) 3 x --G0(_.n0ag--/Ln) re13 p. o%e o mB un oawa m m w n oB om9w% wSaoomloaamqe abee oosa . 150 wiSPeoooowlmdonow %n woo3mu% coaB ws moame Wm 30 SPPoooowwn wad woB oin omonowB0 3o8moams w DSpoooolmm ooonawo o1nm oxswmkeow ENE I CE SU binwoolighE SLG ge om x 2. ily ae Loli fp ee SE en fo. BaF ode, mh Zo ho) s5 c0ePF JEUa Tso SUnEdE aR oS R hoA ansaRenS heEa ae a die ooGkiderSA lSe egRiEaa: eLe i S UE eSdEER en RSG a ee EE Cl SPEEREh soE a nl L Ce CWB E R a = Faeo Logsa A Ie aN io S nh ee P ae EL C TOnL CIE CO REe E e olesLaeeyy B [rp oat EE i : 5 4 2. T ES 5 Rema odorcf Les - -; Tbe 3. eTrrocnesStonciheaisegyetefrrtom control aa8 average specktic growth rate Toa fcoTnoctmn tSrattainonce SwemommwenehnTn ateveemfreraoneocomntw tionen l . 3) Tor em mr sme der awe me er 0.0 (ontrel 0 0 0.020 0.032 0.00 0.00 oom 001s oom oms oon ois oom om 000 sm wom 1 mm 2 Ml "w 0.022 0.017 0.024 0.0 10 & a 17 a 0.005 0.000 0.000 0.000 75 100 100 100 = 1,000 0.017 -0.007 0.000 0.000 15 86 100 100 BIEST COPY AVAILABLE te z iFBgise ne B2 Eia Ci 4 oBkS a AT RsSRa E S dabcn ai abn Rake lohe BE oAUH AR SE ane Tle 3 & Job. EReeCT ESRPS eman Eete LRie ePagReE TlTeR e HR g ) s doe.ii Ae rr GDaam de aa a a Bah le hegi e Bilin ait Udi oh Lilie onal H PalaS ee ERGO Bangg i a a al a a 4 BE E i A a A E can iR er ihee ngSobRSaTE e g EEa RDEeTheea Sea iE ot Bsn SE Ra * N RRR 0018514 BEST COPY AVAILABLE Table 4. Median effective concentrations (EC50s) from toxicity test = WE TkTw cele ! Wn wn - - } Man ww een spaties - Ww wn ew bpasrtatnte ue F L r 4 E m CoH 1. 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Tenecatucs sessed daring tonicity tart oRt tAroEniT TerS re sSeErBS o na 3 ino : 0 A . .3 "nnaa BEST COPY AVAILABLE eh we LL ae Grit Gah das SHR SRE od pe i en Forfa RRR A eSN e Sasi Toa Lae GREENE =ial H a ERO a deaR n CaSERRCSp A E tL ENR ANE p=$r SN e ahd elt ia aan Lc i iRaEf EDE5L E ( aRA EREEORR ER e BlB e e Rn E c Le UBaE LL E AE e - - ) I, 001813 ET ------, . i& 5. BEsT OPY Ay,A |: Table A.2. 5B measured during toxicity test LAB, i Fr - * TaEeT pene nn a } Li i ; : . SE - 33; HI "uo a"Ho: ooownpee ik; id "wo fe iSEEs 1 3 Joy Siegr HoH Hoo wo a woo BH +o Sree 5 Ii Bl LCoc B am ReR Cg on AR Ee a L rs leA SER Sala an nea 7 | ena Gio on Ch elea Ss Ee ar | E03 i P ; LR Nh LAER | i