Document ZJx9GGOBk9pgJDMeGrvyZx72Y

Study Sponsor: Issu e Date: Docum ent Reference No.: ATOCHEM 4-Jun-92 AR226-2972 n. Acute Toxicity of Daphnia Magna Author(s): J.C. Bautonnet A.M. Petit English Translation of Report Sum m ary for DuPont Chem ical Solutions Enterprise 27-Jan-03 Company Sanitized Does not contain T SC A C BI ATOCHEM Levallois Applications Center Analysis - Environmental Services Eco-toxicology Laboratory TEST REPORT INHIBITION OF DAPHNIA MAGNA MOBILITY according to standard ISO 6341 Substance studied : Name : Batch reference : C A L sam ple reference : Person responsible for test : Person carrying out te st: Date of definitive test : Date report written : Results J. C. B O U T O N N ET A. M. PET IT 1/6/92 4/6/92 Application of standard test IS O 6341 (French edition 1989) to the substance referenced above has given the following results : (calculation by linear regression, limit values at 95 % ) The C Efl) 50 24 h is : C Efl) 50 > CEO) 50 < r= 8.94 6.57 12.17 0.987 ma/l ma/l ma/l The C Em 50 - 48 h is : CEfl) 50 > CEfl) 50 < r= 3.78 3.00 4.76 0.994 ma/l ma/l ma/l Additional results : Minimum concentration corresponding to 100 % immobilization in 24 h : Minimum concentration corresponding to 100 % immobilization in 48 h : > 75 ma/l 75 ma/l Maximum concentration corresponding to 0 % immobilization in 24 h Maxim um concentration corresponding to 0 % immobilization in 48 h : : 0.5 ma/l < 0.5 ma/l Company Sanitized. Does not contain T SC A CSt Substance studied : Validity clauses : Reference substance (potassium dichromate) : The CEm 50 - 24 h is : CEm 50 > CEm 50 < r= < 0.92 0.83 1.02 0.997 mo/l mo/l mo/l (normal values : 0.9 to 2.0 mg/l) D issolved oxygen content in the lowest substance concentration G iving 100 % immobilization at the end of the te st: 7.90 mg/l (m ust be > 2 mg/l) Percent immobilization in the control containers: 10 % (m ust be s 10 %) Preparation of mother and test solutions : W eigh : 30 m g in 100 ml of dilution medium (3 g/l) Dilution : 1/30 :10 mg/l Biological information : The test w as carried out daphnias with an age le ss than 2 4 h (selected by sieving). The growth (culture) w as effected in A S T M medium; the food w as algae collected in the exponential growth phase (Selenastrum capricornutum ). Other pertinent information for carrying out the test : Company Sanitized. Does not contain T SC A CBI Elf Atochem S.A. Centre ('Application de Levalfois le , 5 Juin 1992 Ji $$ NOTE A L 'ATTENTION DE Copies : M. C O R P A R T Mme BAKES Labo Ecotox OBJET : R a p p o r t d'essai Vo- - j o i n t l e r a p p o r t d ' e s s a i d e l a t o x i c i t a i g u du vis vis de la daphnie. v JC. BOUTONNET Company Sanifized. Does no! contain T SC A CBI ATOCHEN! Centre d 'A p p iication de Levafiois Service Anaiyse/Environnement Laboratoire d'Ecotoxicoiogie R A P P O R T D 'E S S A I IN H IBITIO N D E U M O B IL IT E D E D A P H N IA M A G N A selon ia norm e ISO 6341 Substance tudie Nom: Rfrence ou lot: Num ro d 'enregistrem ent C AL: Responsable de l'essai: J.C. B O U T O N N E T Ralisation technique: A .M . PETIT Date essai dfinitif: Date rapport: 1/6/S2 4/6/92 R su lta ts L 'application de la norm e IS O 6341 (dition franaise 19 89 ) la substance ci-d e ssus rfrence a donn les rsultats suivants: {calcul: r gre ssion linaire, valeurs lim ites 9 5 % ) La C E(l)50-24h est de: C E(I)50 > CE(!)50 < r= La CE{!)50-48h est de: C E(I)50 > C E(I)50 < r= 8,94 6,57 12,17 0,987 mg/l mg/l mg/l 3,78 3,00 4,76 0 ,9 9 4 " 3/1 mg/l mg/l Rsultats complmentaires: Concentration m ini correspondant 1 0 0 % d'im m o. en 2 4 h: Concentration m ini correspondant 1 0 0 % d'im m o. en 4 8 h: Concentration m axi correspondant 0 % d'im m o. en 2 4 h: Concentration m axi correspondant 0 % d'im m o. en 4 8 h: >75m g/l 75mg/i 0,5m g/l <0,5m g/l Company Sanitized. Does not contain T SC A CB! Page 1/2 Substance de rfrence (bichromate de potassium): La CE(I)50-24h est de: CE(I)50 > CE(I)50 < r= 0,92 0,83 1,02 0,997 (valeurs normales : 0,9 2,0 mg/I) mg/i mg/l mg/l Teneur en oxygne dissous dans la plus faible concentratin d substance donnant 100 % d'immobilisation en fin d'essai: 7,90 mg/l (doit tre > 2 mg/l) Pourcentage d'immobilisation dans les rcipients tmoins: 10 % (doit tre < ou = 10 % } Prparation des solutions mres et solutions rl'egeai- Pese:30mg dans 100ml de milieu de dilutions(3g/i)7 Dilution: 1/30 :10mg/l Informations biologiques: L'essai a t ralis sur des daphnies d'age infrieur 24 h (slectionnes par tamisage). L'levage est ralis dans du milieu A ST M ; la nourriture y est constitue d'algues rcoltes en phase exponentielle de croissance (Selenastrum capricornutum). Autres informations relatives au droulement rte l'sRsai- Company Sanitized. Does not contain TSCA GBl * Company Sanitized. Does no! com** f e r * <"