Document ZJ9nbomvwLjmnndRoz84QJe70

Internal Correspondence de: M. T. Case - 218-3 E. G. Lamp recht * 218-3 W. C. McCormick - 220-2E-02 V. Pothapragada - 236-3A R. A. Prokop - 236-2B L. D. Winter - 236-2B To. E. G. GORTNER - RIKER - 218-3-3 From: T. R. MATHISEN - COMMERCIAL CHEMICALS - 236-2B Subject. ANALYTICAL ANALYSES OF SUSPENSION OF FM-3924 Date: April 8, 1981 INTRODUCTION: Reference: CCD Analytical Method #G-29-0381 Four samples of corn oil with suspended FM-3924 were received on March 16, 1981 from E. G. Gortner. The four samples labeled Placebo corn oil, T-2999 COC 25 mg/kg/day FM-3924, T-2999 COC 2*5 mg/kg/day and T-2999 COC 0.25 mg/kg/day were submitted for mg/ml concentration of FM-3424, for use in a rat feeding study. The samples were run by a GLC method using a methanol extraction and an internal standard. GLC RESULTS: Sample Placebo Corn Oil T-2999 COC FM-3924 0.25 mg/kg/day T-2999 COC FM-3924 2.5 mg/kg/day T-2999 COC FM-3924 25 mg/kg/day Mg/Ml FM-3924 No FM-3924 detected 0.0459 mg/ml FM-3924 0.4694 mg/ml FM-3924 5.136 mg/ml FM-3924 SUMMARY: All samples were within 10% of target and showed good reproducibility. The solubility of FM-3924 was found to be low. Standards used show signs of saturation at 0.5 mg/ml level. It was found that sonification would dissolve the FM-3924 into the corn oil. But after several days the 5 mg/ml standard showed some crystalization, but after sonification it would re-solubilize. The crystalization could cause problems in the feeding study c o m oil. TRM/hc