Document ZJ92XoZZ2LQY9Vo75yaYqK1v7

0750 ACUTE TO XIC ITY TO FISH TEST SUBSTANCE________________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203CE. (1Octanesulfonic acid) (CAS # 2795-39-3). Rem arks: The 3M production lot number was 519. The test sample is FC203CE (2/21/89). Current information indicates it is a mixture of 1.84% PFOS, 30% diethylene glycol butyl ether, 60.23% water, 2.56% Sultone foamer, 3.1% sodium octyl sulfate, 2.1% polyoxyethylene monooctylphenyl ether, 0.12% sodium lauryl sulfate, and 0.05% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluomchemical component o f the test sample. ' METHOD:_________________________________________________________ M ethod: OECD Method 203 and U.S. EPA Method EG-9 Type: Acute static renewal GLP: No Y ear com pleted: 1989 Species: Pimephales promelas Supplier: Aquatic Bio Systems, Inc., Fort Collins, CO A nalytical m onitoring: Temperature, pH, and DO Exposure period: 96-hours Statistical m ethods: Not given. Test fish age: Juveniles Length and w eight: Average length = 19.9 mm Average weight = 0.06 g (wet) Loading: 0.2 0g fish /L Pretreatm ent: None Test conditions: Dilution w ater: Carbon-filtered well water Dilution w ater chem istry: Tem perature: 20 C DO: 9.6 mg/L pH: 8.0 Lighting: Not given. Stock and test solution preparation: The fast material was added directly to the dilution water on a weight/volume basis. Concentrations dosing rate: Daily S tability o f the te s t chem ical solutions: Not noted Exposure vessels: 4 L Pyrex glass cylindrical tanks containing 3 L of solution. Num ber o f replicates: 2 001094 Num ber o f fish per replicate: 10 Num ber o f concentrations: one plus a blank control W ater chem istry during the study: pH range (0-96 hours): 7.9 - 8.0 (control exposure) 7.9 - 8.0 (1000 mg/L exposure) Tem perature range (0-96 hours): 20-21 C Dissolved oxygen range (0-96 hours): 7.7 - 9.6 mg/L (control exposure) 3.8 - 9.6 mg/L (1000 mg/L exposure) RESULTS________________________________________________________ Nom inal concentrations: Bk control and 1000 mg/L Elem ent values: 96-hour LC50 - >1000 mg/L Element values based on nominal concentrations. Rem arks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_________________________ __________________________ The test sample 96-hour LC50 for fathead minnow was determined to be >1000 mg/L. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY___________________________________________________ Reliability: Klimisch ranking 2. Testing meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES___________________________________________________ _ This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number G 1 3 5 8 -1 ,1989. O T H E R ___________________________________ '_______________________ Last changed: 6/26/00 001095 i^V J.nUIW^'UAL, Lirt-DUKAiUK FISH TOXICITY DATA SHEET DEFINITIVE (FULL-SCALE TEST) TYPE T E S T f ^ t o e ^ ^ i d a / b H E M I C A L TESTED ZLfrlliX___ 2 jJ L /f T/YT ,57? TEST m m im M lm iM m u l___ s m n A T F . RECn ^//<r/ra BATCH NO.^ 2 AVG. WT.aAteJ^ilSgrams AVG. SIZE r t ^ ^ h n . OF OGRANIS-IS PER TREATMENT /Q LOADING FACTOR ,,.Qu^ g/L FISH DISEASE TRFATMF.NTTAiME____________ . TEST VESSEL: ,.4. .Liter Ryxex11 glass .cylindrical tank TEST SOLUTION voijime & t. TIME STARTED__ QQdM DATE STARTED s /z t/R ? DATE COMPLETED 3T//<T/fr? DILUTION WATER Carbon-filtered well water (Temp. Z Cr D.O. mg/Lf pH ) A N A L Y S T LC5Q(95% Confidence LimitO > /n nOjM^/ / j _________ Tank Initial Total _____ 2L-^Qurs__ 48 Hours 72 Hours 96 Hours No. Cone. Amount Total Nc. PH D.O. Total No. pH D.O. Total No. PH D.O. Total N. PH D.O. mg/L Added Dead mg/L Dead mg/L Dead mg/L Dead mg/L 3 X Control O 1R *2>,Zj > 7,7 1U O fitQ ha O in TT Contimi 1ft * 1 lOOO - W ) 0 f\ 0 i %,l o O IA n ti fiO Ho O O 1R in f 2j lOCiO ryOCD Q (tO 7 fi sV G M Test Temp. C Initials Date Z jO s /i 2 /e ci xo T )'3 s /a f f JU s liL f f f JD S-//S/8? COMENTS: ^l^ls/lduall w/a-Wini a m a r a i Are^ )^ |.^ulr^ W0^i>Wig i^EUi u(fiter: UMlaLpU- n [ (T )vu<}Lj *t.q.----------------------------SECONDARY EFFECTS (OTHER THAN MORTALITY) lank No. X IT 1 Zj Symptoms or Reactions 24-Hr. y ,c<i< vC ^ y rotai Numb Sr A tle c te d "1' 48-Hr. 72-Hr. y C 'f' 'J & 96-Hr. 50 v<r Test methodology conforms with the following two test protocols: (1) OECD #203 "Fish, Acute Toxicity Test". d c q t m D\/ a \/aii A r-. r- (2) US-EPA #BG-9 "Fish Acute Toxicity Test". * COPY AVAILABLE 001096