Document ZBOm6bg1OB27XV4zybp5XELp

AR226-3128 -_-- pemmus TRADE SECRET Study Title H-24119: Skin Irritation Test in Rabbits Laboratory Project ID: DuPont-3268 AUTHOR: Carol Finlay, B.A. STUDY COMPLETED ON: July 30, 1999 PERFORMING LABORATORY: E. I. du Pont de Nemours and`Company EHalskkteolnlRLoaabdo,raPt.oOr.yBfooxr T5o0xicology and Industrial Medicine Work RequestNoose{NEevwNiskTDower 197140050 swvics CoveNovos JD) `Company Sanitized. Does not contary TSCA CBY LS 2200190n : SinimamTeosw im Rais ontesiRaDpt uruons e26ss CERTIFICATION Wfreo,mtthheisunsdtuedrys.igned, declare that thi report provides an accurate evaluation of the data obiained Revives: _ MGreeerEsSsauaret ned byStd Dircior Ca any 230Q0uty % 119999 20 hheell 1177 --- omPanY Santas. boos otcontain rsp car a SS H-e2411n 9: Skl in Imitm ation TeeinRi sabbitto s nTeinRabbDwi uPoonts w326s8 TABLE OF CONTENTS Page SCTERUTDIYFIICNAFTOIROMNAcTIoOmNsc ssmssso sssssss ssssssm sssssso sssssms snssnd STUDYPERSONNEL crs SUMMARY womans INTRODUCTION css] MATArERTIESAULSSUBASNIADNGMEETHw ODS c oo es va] Be ARIA HUSDNGIY rns 7 RESULTS AND DISCUSSION coves CONCLUSIONSwrested) RECORDS AND SAMPLE STORAGE wc. - 2J 1.. ID NDIR VIDSUCA AALLSEKFI IONRIZ RSRCIOTRAE ITNIGONSSKCIONRIRERSIATANTDIOSNK.I.N.RESPONSES OBSERVED |1 INRABBITS sss 12 -Y Company Sanitized. Doos not contain Tc cay EE201. Sn rn Testi 9h Colletve Nomencle STUDY INFORMATION -- ] Synonyms/Codes: H-24119 atl Numer: 24119 CAS Reger Numer ] _ Submits Nowook Nunc IESNENY Purity:{ory foam nn | LE --| Stability: ---- Tcohnedittesitonssuobfstthanecestaupdpye;anroedevtiodbeencsetaobfleinusntdabeirlitthyewas Soonsor Vh1iilsninPgoonnt dse NaemsourTsonsd Company Stu nedCompltes ul 10,1(s9er9epo9rt cover page) Life IntedCompicte: July 1, 1999 sly 16, 1999 CompanySnitz oes etconn 150m 01 -- H24E119E: Ski-- n Iriation Test-- in Rabbis ---------- DuPont-3268 STUDY PERSONNEL Study Director: Management: Carol Finlay, B.A. Judith C. Stadler, Ph.D. Primary Technician: Carol Finlay, B.A. `Toxicology Report Preparation: Amy L. Williamson Laboratory Veterinarian: WandaL. West, D.V.M., A CLAM -Y `Company Sanltized. Does not contain TSCA CBY H24119: Skin Imitation TeR s in Rae bbits _ DDwuoPowntd32e6s8 SUMMARY aFAn-pd2p4rc1oov1xe9irmewadatsweieltvyhala0.u5 asmteLemdio-ffoocrtchalecuutsetisevtesskudibrnesstisaminintcgaetfiwooarnsapoa4tp-ephnlotiiueardletoixtnpho5seumrasehl.aevNeAdpe,pwrionZtxeaiacmtlaastnkedilnWyohf1i,tc2ea4c,rha4br8baibtbsi.t a7n2dhootuhresraefvteirdernecmeovoafldoerfmtahle etefsftecstusbsatnadncwee,rethsectoersteditaecscowredriengevtoaltuhaetDerdafiozreeSrcyatlhee,maT,heedem,a.and adjacent areas of untreated skin were used for comparison. 2N4o heoruyrtshaefmtaerwtaesstosbusbesrtvaendceinre4morvaablb.itsAatn4d8mialndd e7r2yhtohuermsa, exhibited slight erythema, and 1 exhibited mild erythema. w3arsabobbistesrevxehdibiinte1 dranbobietryatth1emaan,d Desquamation was observed in | | rabbit at 48 hours and in 2 rabbits at 72 hours. No edema was observed during the study. Under the conditions of this study, H-24119 was a mild skin irritant, - Company Ssaanlized. Does not contain rsp cay - EE HE-24119: SkE in tation TE est in RabbiE s --------D-- uron. 3268 INTRODUCTION hthee sphuarvepdo,seinotfactthisskisntuodfyNweaws tZoeaevlaalnudatWehithteesrkaibnbitism.itation potentialof H-24119 when applied to MATERIALS AND METHODS A. Test Substance _ The es substteasntcseu,bsHt-a2n4c1e19w,aswaisnsvupeplritoetdmeibdyxtehefsoprocnaschoasrama test substance appeared to be stable under the conditions of the study. ON No evidenceof instability, such asa change in color or physical state, was observed B. Animal Husbandry -- cHYaeogweuistn.tg,EaNdaueclhwt rHJaeMbrb:sie(tyN.wZaTsWh)aeMssrBaiRbgbniNetdeswaweuZrneeiaqlhuaoenudisdeWednhtisiitfneigclarytaibiobnnitsnsuuswmpeberneedrerdwe,hceisictvhaeidnwlafesrssormsetcHeoealrr,deewdiMraorenl-amanecdsa,frd caaffcihxerdabtboitsheacragwei.th`Tahewalutste3indsiogiltusbloefmtahrekeirde.ntTifhiecartaibonbintsumwbeerre wofefreerewdriatptpernooxnimtahteelinyside of ad1nu2ir5minagglrsatmhwesersotefudPiyn.MsItWoNcaukttser.irtwioansIanvtaeimlaatbiloenaald,lIinbci.tuCmeretxicfieepdt HdiugrihnFgitbheereRxapbobsiutrLeapbeDriieotdw5h3il2e5 tdhaeily bHaadacmstikenerilislatLecarobeuodnrtbasytoatrnhyedhLfaaosrboatrnhaetaponrrieymsaVelentcheeeraiolnftahcroimlaoinnf.iotrWomarsti,enlgreapsdra,omagpnrlademso.tahrTechrpicesornpitoradomigicrnalaalnmytsias.nmaolAnydizctetodirofeondraattonyrd.a] rsseaacnmoiprtdlaste,isonfArnboyimmtafhlreeasfgheleeywdawissahsceehrresid.ficeaDdgaebtsyaatfnhrdeocmmaatgnheuifsraapccrtkousgraerrraemtopaermreeioemdtaiicsnaptlelacyiinfaienedadlsyneuzpteardriatttioeolneyanlsfurrreoeqmuaisdtreueqdmyue.anttes ihaandvdeetnoicnbteeersffuregergeeedoswtafiitnhlgisttthhoaelfrcsesopuenlcttiasfmioiefndatcnhoitnsstsawtmeuidrnyea.nptrse.senOtninthtehebafseiesdoofrwtahetseerainnalaymseosu,ntthsewrehi1schpomay CwRoeanbrdbeiittmisaoinwnsetroaeifnqtehudearoranonotaimnisemdew,re-rwceeointgtahrroegldelt,eeadd,nfd1oo2r-bhstoeeumrrpvleeirgafhtotur/r1ge2e-nohefro2aulr0hdCeaarlkt1hcyfcColrea.n11dEdnraevylisar.toinvAemnehinumtmaailldirtoyoomfs 3an0d%/o=r d1u0r%a.tioEnxctourhsaivoensadovuetrssiedleytahfefseectreadngtehse wvaelriedijtuydogfetdhetoshtaudvye.been of insufficient magnyiude - ompany Sanitizad. Does not contain TSCA ca! SH2e4119: SkniniImmitsatoionnTTeestrien RRababibtse -_ C. Protocol DDuupomnta326s8 O10nexthpeodsaeytphreiosrktinoftrroemattmheenstc,aptuhelhaarirtoofth6e mlaulmebaNrerwegiZoenaloafntdheWhbaictke. raTbhbeitsbowdayswceliogshetlys sohfatvheed rabbits ranged from 2423 10 2800gramson the day of treatment. daEuparpcirhnogxriatbmhbaaitttetlwiyamse8"dpilxdac1n8eo"dt. ihnTatvhoeeaarscatcboebcsikstswthroiefcmohaoidhnoaerddwbianeteetnrh.efistAttopecdpkrwsoixtthihmraoautpgeihelocyueot0.ft5hrmeuLbebxeporofssHuh-re2ee4tpi1en1rg9i,owdaasnd ai{0mpripetltaiateirdnddgeivrtaaeppceto.lryaoTthnieotnrhuaebnsbdkeirtnosbhkeeeneetepianttghhewaat2ess-titnshcuehbnsgwtaarunazcpeepseinqducaaorrneotuatnchtdatwtwihaethsanthiheemladslkiinannpdwliastcehecouwuritetduhnnwdioutneh- clips pressure. backs of One these other test rabbits. substance was tested concurrently on a separate, localized test site on the aaAnnpddprgtohaxeuizsmekaitsnequaltayrt4ehsehwcoueorrmseeratfshteeonrfartpehpmeloigvcaeaudtz.ieonosTqhfueatrhteeessttwessaittsessumbwasertrkaeendcgeew,nitttlhhyeawrauwbsabhteeerrdpsrwhioetoehftipwneagnr;wmawsrwaalptopeoirsnetgnyesd, femove excess their cages. test substance. The skin was gently patted dry, and the animals were returmed to - 0eAvpaptlhrueoaxDtiremadaiftzoeerlSeycray1l,teh2e4(m,Taa4,b8l,eedae1n)m.da,7T2ahnheodauodrtjshaecareftneetrvairrdeeemanoscveoafloftohfdetehurnmetarlteeasettfefsdeutbsssktiaannnwcdee,wreetrhueessetecdsotfrsoeirdtceasocmwcpeoarrrdeiisnogn. r`oauObtnbseiitrdsaebwbteihtreewteaaslstssoriteee.mxoavTmheiidnsefddreofrmomratchlleiinsriticutadaltysibiongencwsaauossfebteotxlhiiecerivatebydbaitttoeehvxee:hridybuiedteetrdomdaselhraemvvaialnlguiaratrbiirotaanst.iioonTnsho,enTahned were weighed on the day of treatment and at the last dermal evaluation. rabbits RESULTS AND DISCUSSION N2L4eoxhheoirubyritstheaedfmtaselrwigtaehsstteosrbuysbteshrtevamenadc,eianrne4dmor1avbaeblxi.htisbAiattne4dd8mmiailnlddde7er2ryyththohueermmsaa,.w3aDrseasbobqbiustaesrmevaxethdiiobiinnte1wdarsanboboibetsreayrttvh1eedmaan,d r(1a7br2bahibotbuiertxsha)ti.b4i8Itnehddiovuwiredsiugaahlntdsklioinsns2iorrfiatbaabptipitrosnoaxstic7mo2artehesoluayrrse2.9p%orNefosienneitdteiedamlianbwToaadbsylowebe2si.egrhAvtebdsyudmustmruiadnrygyttoehrfemssitknuiadntyi.onOne responses is presented in the following table. -Y Company Sanitized. Does not contain TSCA car H24119: Ski riaion Tes inRReabbits -- SUMMARY OF SKIN RESPONSES TO H.24119 DwuPoontw326s8 -- Fo --mvmHOeUvRSaAFTE--R TT EST SUBSTANCE REp MOVAL RESPONSE[LT aT 7To[wT5] Ro bate TTu17s05T Tos s 1T1w 5[ TuussTToossToossTa osT Toosn]] CONCLUSIONS Under the conditions of this study, H-24119 was a mild skin irritant. I(lfaEtbEehCleisnDgiorteefscdttaisnvcgeore9er3so/ua2rs1e,stAuonbbsneteaxunscVeeIds),pfouHrb-lE2iE4sh1Ce1/d9OipEnrCtohDbeabcOllfayfsissciihfaoilucalJtdoiuonrno,ntaalbcoecfocrlEdausirsniogfpiteeoadtnahseCoagnmumiduenittoitehse further data proves otherwise. mitant apless RECORDS AND SAMPLE STORAGE ~ NkSenpweoacwrinkm,easnDstehlGeiafwE.aaprp1e.l,idcuoarbPlaoetn)tI,rrdoneawMNoedaumtnoatu,araisnndaRtnehdceoCfroidnmsaplMaraennpyoargRteewmcieolrnldtbs,eMWraienltmaaiignneegmdteoatnnt,HaDCseeknlealwlaLraebo(rfaotromreyr,ly ter). -Y Company Sanitized. Does not contain TSCA CBI ---- H-24119: Skin itaton Test in Rabbits Dupont3268 TABLES -Y Company Sanitized. Does not contain TSCA CBI H26119: Skin rion Test in Rabbis Dupon268 TABL1E DRAIZE* SCALE FOR SCORING SKIN IRRITATION EvaluationofSkin Reactions Score E Eerryythleigmhat aenrydtheesmcaha(rafrolrympaetricoenp:tible)T ... SMN eveore erdyttohseeR emvearr(ebeeertayrtehdtenmesaso.).t.o sight cchar formation (muri in dep)...EY412S(+eS0vliegNrhet)D EdSNreOymalIigfRhotrmeadteimoan:(barelyperoeptibley...s ......___ s T s Y ,0 SiSegdvheetretedeedeeemdmaeam((eardagi(essieosdfemdaorrSeeaPwtheOalnT1dO.e0fmXinmIe MbexyAtdeInediilnngYbeyoLnOdT)t.he..sesraoifsing)... 32((VMoritrat) CRORE) tess e 4 (SEVTE) ~ AbbrAe-vi=a=tioANnbosrEafodffeeodctther dermal effects areL:1 == ISonuaghing CB == EBslcahnacrhing D = Desquamation NR == NReacwroAsriesas S = Epidermal Scaling GF == FFisisursinsgwiuthrBlieendigng H = Hyperkeratosis XT == CoThmipckoeunnindgAchered o Skin * DPEpdii.t4o6er.i5aJ9l.HC.om"mDeirtmaolftThoexiAiyv."iAapprtaoiifsaoFloonofdthnedSaDfreuoOffChfeimciicoaltie FUosoedss,eDrssandCospmia,sTThhee - `ompany Sanitized. Does notcontain TSCACBE - H-24119: Skin Imitation Test in Rabbits DuPont3268 TABLE 2 INSDKIINVIRDEUSAPLOSNKSIENSIORBRSITEARTVIEODNISNCROARBEBSIATSND NURMABBBEIRT TaTas To Fs1215 1 TT [as T5] I ToToo 1] A Le oe T7C 01010IoTooCTToo] SUHBOSUTRASNACFETREERMTOEVSATL - NRUAMBBBIETR "1T24|48|75| ETT sal ES I | E57 IE ES I I Pn Css 1 Symbols are defined in Table 1. = Company Sanitized. Doss not contain TSCA CBI - --