Document ZBD7MJx52pG2qraNKa32jNjMJ

MICROBICS MICROTOXC TOXICITY TEST TEST SUBSTANCE Identity:Perfluorooctanesulfonylamido(ethyl)acetmaatye;also be referredto as PFOSAA, FC-128 or as the major component of FC-1 27, FC-1 29, FC-1 09,FC-1 09-X or Rl 904. (Glycine,Nethyl-N-[(heptadecafluorooctyl)usIfonyl]p-o,tassium salt,CAS 2991-51-7) Remarks: The testsample isFC-129 referredto by thetestlaboratory as Rl 904. Currentinformationindicatesitisa mixtureof42% test substance,32% water,14% 2-butoxyethanol4,% ethanol,and 8% C4-C7 fluoroalkyclarboxylatecompounds. Test materialwas from 3M production lot930014, mfg date 3/97. The followingsummary isabbreviated because the testsample isa mixture of the testsubstance in a waterlorgank solvent solution. Data may not accurately relatetoxicityof the testsample with thatof the testsubstance. METHOD: Method: MicrobicsMicrotox@"BASIC" Procedure Test type:Staticacute GLP: No Year Completed: 1997 Species: VibriofischerfiormerlyPhotobacteriumphosphoreum Analyticalmonitoring:pH and osmoticcondition Replicates:Two Statisticamlethods: EC10 and EC50 valuescalculatedby statistical linearregressionprogram providedforMicrotox@ Test organism source: MicrobicsMicrotox@ Reagent. RESULTS Nominal concentrations:Blankcontrol1,25,250, 500, 1000 mg/L Element value: 5-minute EC50 = 354 (316-396)mg/L 15-minuteEC50 = 290 (261-323)mg/L 30-minute EC50 = 274 (248-302)mg/L Allelement values based on nominal concentrations. Remarks: Testingwas conducted on the mixtureofthe testsubstance as describedinthe testsubstance remarks field.The value reportedapplies to thatmixtureand not thetestsubstance. No attemptwas made to determinethe impact ofthe presence ofthe organicsolventsor what portionofthe toxicitcyan be contributetdo the Perfluorooctanesulfonylamido(ethyl)acetate. CONCLUSIONS The FC-1 29 30-minute EC5o forVibriofischeriwas determined to be 274 mg/L witha 95% Confidence Intervaolf248-302 mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota, 55133 DATA QUALITY ReliabilityK:limischranking2. Testingmeets allcriterifaorquality testingb,ut lacksanalyticaclonfirmationoftestsubstance concentrations. There isa lackofcharacterizatioonfthe testsample. REFERENCES This study was conducted atAScl CorporationofDuluth,Minnesota atthe requestofthe 3M Company. OTHER Last changed: 5/17/00 AScl Corporation TEST--.., C( L4 DATE TECHNICIAN,j4,k., eftc,ctive6:/1"3 par" 7 of It SponnrImsSsouor. Sponsor Study ED#: AFSAStSuCdIySItudyM#: ScSTSetpsu0tdyDirector: po TestSubstance: Ref,eerence Substance.es Test Organism: Teyspte of Tdt: Test Guideline: InLfe rG01e0Phase Dates: //Pt--T6P, II 4w STUDY ASdCOV.0!4.lbo SOP No.-.'IXT-0-7 P."LA.Cr 4e-s Providecomments below ifany additionaolbservationsare made or data provided: Providean explanationbelow ifthe testisunsuccessful: L7 Effcctive6:fl"3 Page 7 of a MICROTOX DATA*REPORT Basic Test FILE: 97070901.K5 R1904 dark yellow in color;consistency of cough stock Test Time: 5 minutes Osmotic syrup. '2000mg/l Adjustment;NaCl NUMBER ------- Control Control IO/IT ------------ 96.99/ 78.33 110.50/ 89.45 CONC. ---------- 0.0 0.0 CR/GAMMA --------- 0.8076 # 0.8095 # EFFECT AV q DIFF. ------- ---------- 1 116.03/ 67.32 125.0000 2 110.55/ 60.09 125.0000 3 122.19/ 53.45 250.0000 4 108.70/ 53.42 250.0000 5 103.95/ 35.95 500.0000 6 110.81/ 37.84 500.0000 7 111.12/ 26.72 1000.0000 8 109.79/ 27.90 1000.0000 0.394 # 0.488 # 0.848 # 0.645 # 1.338 # 1.368 0 2.363 # 2.182 # 28.2 @2.8 45.9 39.2 57.2 57.8 70.3 68.6 30.5 2.3 42.6 3.3 57.5 0.3 69.4 0.8 CR COCORRECTION FACTOR # Used for calculations = 0.8086 r EC50 354.0 Z-OttV5% CONFIDENCE RANGE-316.3 TO 396.3) Signatur@-. CONTINUED TO NEXT PAGE.:. TEST DATE; TIME: S- ?,:7 jig i6 FILE:97070901.K5 PAGE 2 10 3SLOPE 0.7992 3 3 G3 A3 M3 M3 8 A3 3 6 1 >zzzzzzzzzzzzzzzzzzzzzzzzzzzzz_._ ?DDDDDDDDD? 3 3 3 3 3 3 3 3 3 < 3 3 3 3 4 3 3 3 3 2 3 3 1 3 3 3 3 3 3 EC50 3 3 3 10 (-,IDDDDDDDDDDDDDDDDDDD- DDDDDDDDDD. DDDDDDDD- DDDDDDDDDDDDDDDDDDDY 10 100 CONCENTRATION 1000 IOE4 ESTIMATING EQUATION: LOG C = 1.2247 x LOG b 95% CONFIDENCE FACTOR: 1.11940 FOR EC50 COEFFICIENT OF DETERMINATION: R-2 0.97877 +2.5491 FILE: 97()7()901.KI5 MICROTOX DATA REPORT Basic Test R1904 dark stoc@--. Test Time: yellow in color;consistency 15 minutes of couah syrup. 2000mg/i Osmotic Adjustment:NaCl NUMBER ------- Control Control. IO/IT ------------ 96.99/ 72.5o 110.50/ 81.76 CONC. ---------- 0.0 0.0 --------- 0.7475 # 0.7399 # % EFFECT ------- AV % q DIFF. ------------ 116.03/ 58.65 l@@@5.000o 0.471 # ll().55/52.30 4L25.0000 0.572 # 122.19/ 45.0-@? 250. Or.)O(-f 1.017 # 4 108.7C,/ 44.87 250.0000 0.802 # 0 95 30.48 5,)I:(j).000 1. 5@:-6 # 57 5()0.()()C,,O 1.530 # 36 -q.79/ 4 C)o 2.696 # 2.4e5 # @-o'-ORRECTION FA-,-@TOF-t 32.0 36.4 5().4 44.5 6().6 5 -,--.9 71 @7, 34.2 q 2.2 47.5 a 3.0 5 ).8 7--J,. Nr- I DENR- F,.N'l^,-L, @i,I TO t Lkr e T n- TES*T DATE: TIME: FILE;?7070?01.Kl5 P.ABE 2 10 ZDDDDDDVDDDDDDDDDDVDDDDDDDDDDDDDDDDDDDDDDDD 3SLOPE = 0.7716 3 3 6 .3 A .3 m .3 m3 A .3 .3 6 4 .>3zzzzzzzzzzzzzzzzzzzzzzzzzz .3 DDDDDDDDDDDDDDDDV? .3 .3 .3 .3 7 .3 % .3 .3 CONCEN!-RM-*T"L@"1.@,l DEIDDC,-ODDDL E-@i'TIMATING L C, C 95% CONFIDENL--C PACI'OR: 1.11152 t, +2.462@o FOR E-@-50 COI@l:--c!CIENI- OF DET'r_-RMJ',@4A-!'IL)N, L:jD D C lOE4 -7,-69 - --7 FILE: 97070?01.K30 R1904 dark yellow in stock Test Time: @:10minutes MICROTOX DATA REPORT Basic Test color; consistency of cough. Osmotic syrUD. 2000mg/l AdiLtStment:NaCl NUMBER ------- Control Control. IO/IT ------------ 96.99/ 67.4() 110.50/ 76.81 CONC. ---------- 0.0 0.0 CR/GAMMA ------- 0.6949 0.6951 % EFFECT AV q DIFF. ------- ------------ L 116.03/ 54.30 I2L5. ()()()0 C).,fIB5 7 2 14LO.@J5/ -Ara.04 125.000() 0.599 :1.7 5 - 35.1 o 2.4 49/ 4@.@5 -30.,)i)Oiy 1.(-)05 5 c-' 4 !C)8.70/ 41.06 250.OOGtD ..,84() 45.t@ 47. 9 2 5 27.41 5(-0. 1*.636 62. 6 1 2c?. I ot)oo @78 62. 61 0 C) 2.?9- 7&:7 .2.7c)I- 4 i) 74. c.11.i '-D 69.5'- A.r-@i.:E- 2 4 a 1 COt,@! Tf--,@4EXT TES-I DAi'*E.T-IME: FILE -1?70/'0901.f"'ZO PAGE !C) ZDDDDDDDDDDDDDODDDDOODDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDDD? 3SLOPE 0.80:-9 3 3 -3 0 .3 A .3 m .3 m .3 A .3 .3 .3 .3 -3 .3 6 -4 .3 -3 -.7 OD- L'.D D D D D 0 D C) ?7j D D D D-E,D El . D CONCENTRATION D ElD D Z-1 100(,- DDDDvDDDDDD D 1 (DE4 ESTIt-'iA'r!NG EQ@'ii'-@ITIONL:Or3 C = 1.227? x LOG b 95% CONFIDENCE FAt@"TOR: 1.10200 FOR EC50 COF-FR-ICIENIT 1--(---2 +2.437() MicrotoxO AttachnientA Environmental Laboratory Test SolutionPreparation @ L-r6 -1@f7 ('5,c LR/StudyNumber:-R I-qoL4 Date@rime: -aiAijl;a530 Anaiyst: @IA 1-174- TEST SUBSTANCE: Ct o@ 1. Preparation of Stock Solution or 100% Solution (Iftestinag 100% solutionr,ecordfmalconcentratioans 100% and skiptosectio2n) M,assIVolumeor volumelvolwneofTestSubstance: DiluentUsed. m 2@l;L 1. FinalConcentrationofStockSolution(mgllor % v/v): - BalancUesed-:-@@@@ A I 2. pH Adjustment of Stock Solutionor 100% InitialpH.pH Adjustetdo: pH Adjustewdith: Solution <3L,4 '4. I-4Ll 3. Osmotic Adjustment of Stock Solution or 100% Solution OsmoticAdjuster: uiq c-l- (4cc)-amddgedto -)C)ML solittion Conunents: a-,Aum CY)ltyt(IF jtA ho3 4. Appearance of pH and Osmotically Adjusted Stock Solution I (aY-=AR*"" or OPAQUE.'.-- (Circloene) Color: Comments: i LjI EiLoW 12 ic@0(4 dewv) Ci)L 5. Cb4nmer@is' \j WE11c)(.Aljrc)jt,)( j 119 EEF-M-3.0 Pag2e1of22 ASCI Corpomtion/ASCI-Duluth Environnmtal TestingDivision MICROTOX(D PHENOL REFERENCE -TOXICITY CONTROL CHART July 1997 July 1997 July 1997 23.59 18.26 18.23 20.93 3.769 29.47 12.99 18.0 20.03 3.086 26.20 13.86 15.4 AScl Corpomtion/AScl-Duluth F-nvironmentaTlesting Division MICROTOXO ZINC REFERENCE TOXICITY CONTROL CHART 77@-7 ..... June 1997 3.898 - an - ]Unut Limit June 1997 4.491 4.190 0.412 5.014 3.366 9.8 l@997 4.125 4.168 0.294 4.7@6 3.580 7.1