Document ZB4kXwoZ9gjgExE4wk00pLg8Z

AR226-2930 FOR DU PONT USE ONLY HLR 415-85 HASKELL LABORATORY REPORT NO. 415-85 Title of Report STATIC ACUTE 96-H0U. LC501 OF, TO THE FATHEAD MINNUW, Pimephales promelas E. I. du Pont de Nemours and Company, Inc. Haskell Laboratory for Toxicology and Industrial Medicine P. 0. Box 50, Elkton Road Newark, Delaware 19714 Date Issued: September 17, 1985 Company Sanitized. Does not contain TSCA CBf HLR 415-85 Title of Report STATIC ACUTE 96-HOUR LC50 OFi_ TO THE FATHEAD MINNOW, Pimephalea promelas Summary 1 exhibited low acute toxicity to a warm freshwater fish, the fathead minnow, under static unaerated test conditions during a 96-hour exposure period. The 96-hour LC50 was 2341 mg/L, with a 9558 confidence interval of 1766 mg/L to 2743 mg/L. Report by: : \ > A* - _ ^ y s ?rdy< x, ' James W. Wetzel ' ! Technician ^ V Study Director: T^jJlpW 6-. S+wVoe,Tr. Ralph G. Stahl, Jr. Research Aquatic Toxicologist /- t Mo/Day/Yr l/lT-ISS Mo/Day/Yr Approved by: < *-//! - . L i/ # ' Carol 6 . Litchfield Section Supervisor, Environmental Biology Mo/Dayr SfPmpany Sanitized. Does not contain TSCA CBi Haskell Laboratory Report 415-85 HLR 415-85 Material Tested Haskell No. Other Codes CAS Registry Number Sponsor ymer Products Department Material Submitted by Polymer Products Department Washington Laboratory Parkersburg, W. Va. Test Facility E. I. du Pont de Nemours & Company Haskell Laboratory for Toxicology and Industrial Medicine P. 0. Box 50, Elkton Road Newark, DE 19714 Study Initiated/Completed There are 7 pages in this report. Distribution I^Wpan^ Sanitized. o e sm e o n M n T5CA CBI 3 HLR 415-85 Introduction/Purpose . To determine the static acute toxicity (H-15,866) to a warm Freshwater Fish, the Fathead minnow (PimephaleB promelas). Materials/Methods (Environmental Biology Section Aquatic SQP-TQ2) The test material t H H H was received as an aqueous slurry containing of the active ingredient, All nominal concentrations and the stock solution are based on the weight (density of slurry: 1.2 g/mL) oF the aqueous slurry and were not corrected For the amount oF active ingredient. The test material, was prepared as a 50 mg/mL stock solution in distilled d e i o n i z e d w a t e r ^ T h e pH oF the stock solution was adjusted to 7.0 with 1N NaOH. Appropriate volunes oF the stock were added to 5-liter all glass aquaria and diluted with laboratory well water to yield the desired nominal exposure concentrations in 4-liter Final volumes. Two identical vessels containing only laboratory well water were designated as the Ho0 controls. ' Stability oF the test and control materials in well water was not determined because it is not a critical Factor in static acute aquatic tests. Five juvenile, unsexed Fathead minnows From Haskell Laboratory-bred stock with a 2.08 cm mean standard length (range 1.6 to 3.1 cm) and 0.156 g mean wet weight (range 0.023 to 0.440 g) were randomly placed in the test aquaria. The Fish were maintained at Haskell Laboratory For 126 days beFore being used for this test. Haskell Laboratory-bred Fathead minnows are identified by labeling the culture and test vessels. Fish were not fed For 48 hours prior to nor during the exposure. The test solutions were not aerated, and temperature was maintained between 21.1 and 22.0C. Photoperiod was maintained at 16 hours light: 8 hours dark. Mortality counts and observations were made every 24 hours during the 96-hour exposure period. Dissolved oxygen and pH were measured in the H^O controls and in the low, medium, and high test concentrations at the beginning of the test and at 48-hour intervals during the 96-hour exposure period. Total alkalinity, hardness (EDTA), and conductivity were measured at the beginning of the test in the H^O control. The 96-hour LC50 and confidence limits were calculated by probit analysis (Finney, 1971). All records From this test and the final report will be maintained at Haskell Laboratory for Toxicology and Industrial Medicine or archived at the Du Pont Hall of Records. -4 ammzec, Uvea riot contain TSi HLR 415-85 Resulta Test results for fathead minnows exposed (H-15,866) are presented in Table I. e x h i b i t e d l 5 ^ 5 u c ^ ; o x i c i t y to fathead minnows under static unaerated test conditions during a 96-hour exposure period. The 96-hour LC50 was 2341 mg/L, with a 95S confidence interval of 1766 mg/L to 2743 mg/L. The chemical and physical parameters measured during the test of H-T5,866 are reported in Table II. All were within acceptable ranges for this study. Discu38ion/Conclusion _fCH--15,866) has been tested for acute toxicity with the fathead minnow, a warm freshwater fish. Under the conditions of this test, H V w a s found to exhibit low acute toxicity. Footnotes 1 LC50 = That concentration which is lethal to 50% of a test population during the specified time period. References Finney, D. 3. , (1971). Probit Analysis, 3rd. Ed., Cambridge University Press, 333 pp. 5 Company Sanitized. Does not contain r s n HLR 415-85 TABLE I RESULTS OF A 96-HOUR STATIC ACUTE TOXICITY TEST WITH FATHEAD MINNOWS EXPOSED TO H-15,866. Nominal Test Concentrations (ma/L) 6400 3200 1600 800 400 200 H^O Control 24 Hr. A* 8* 100 100 100 100 00 00 00 00 00 Observed Mortality (S) 48 Hr. 72 Hr. A* B* A* 3* 96 Hr. A* B* 100 100 100 100 100 100 100 100 100 100 100 100 0 20 0 20 0 20 0 0 00 00 0 0 00 00 0 0 00 00 0 0 00 00 Replicate exposure chambers containing five fathead minnows each. - 6- not eontf>'n S'.w-' HLR 415-85 TABLE II RESULTS OF PHYSICAL AM) CHEMICAL PARAMETERS MEASURED DURING A 96-HOUR STATIC ACUTE T Q J O C I T ^ F S T ^ WITH FATHEAD MINNOWS EXPOSED TO H-15,866. Physical/Chemical Parameters Dissolved Oxygen (mq/L) 0 Hr. 48 Hr. 96 Hr. H 0 Hr. 48 Hr. 96 Hr. Total Alkalinity (mq/L as CaCO^) 0 Hr. EDTA Hardness (mq/L as CaCO^) 0 Hr. Conductivity (umhos/cm) 0 Hr. 6400 (Hiqh) Nominal Test Concentrations 800 20 Controls (Medium) (Low) H,,0 8.4 6.6* --* 6.9 7.0* -- 8.4 6.7 6.2 7.3 7.3 7.4 mm ___ 8.3 8.3 6.8 7.5 5.9 7.5 7.3 7.4 7.3 7.3 7.3 7.4 84 _ 76 166 * Measured at 24 hours due to total mortality. - 7Company Sanitized. Does not contain TSCA C ? l