Document Z8oX0G2yVGeEnGQQqJOMr3Yap
AR R26 -- 0&/3
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST
TEST SUBSTANCE
Identity:
A mixture containing referred to as PFOS,
pFeCr-f9l5u,oroorocatsanaescuolmfponoanteen,twohficFhC-m2a0y6CaEl.so
be (1-
Octanesulfonic acid) (CA#S 2795-39-3).
Remarks: The 3M production lot number was 502. "0T.h9e2t%esPtFsOaSmp,l1e5i%s FdCi-e2th0y6lCeEn.e glCyucrorlenbuttiylnfeotrhmeart,io7n6.in0d0i%cawtaetserit,is4a%muirxetau,re of m1o.n2o8o%ctSyullpthoenneyfloaetmheerr,, 10..5056%%ssooddiiuummolcatuyrlylsusluflaftaet,e,1a.n0d5%0.p0ol5y%oxtoyleytthriyalzeonlee.
The following summary applies to a mixture with incompletely
rcehfalreacctttehreizteoxdiccoitnyceonfttrhaetifolnusoorofcihmepmuirciatliecso. mDpaotnaenmtaoyfntohte atcecsutrsaatmeplyle.
METHOD
Method: OECD 209
Testtype: Acute static
GLP: No
YAenaalrytCiocmapllmeotneidt:ori1n9g8:9 Dissolved oxygen concentrations, pH, and temp.
iSnthaitbiisttiiocnalinmreetsphiordatsi:on Rreatseu.lts were determined by calculation of the % Test organism source: Activated sludge mixed liquor collected from the Metro Waste Treatment Plant, St. Paul, MN.
Test coDnidliuttiioonn:water: Millipore Mill-QTM water RSyenftehreetniccesaenwdagtees:t spoelruOtiEoCnDprmeeptarhaotdion: A stock solutionofthe r5e0f0ermegncien s1u0bsmtLan1cNe,Na3,O5H-,dicdhilluotreodphteon3ol0,mwLawsitphreMiplalriepdorbeyMidilsis-oQlvTMing water, then brought to the point of incipient precipitation with 1N HSOs and diluted to 1Lwith Mill-QTM water. The pHofthe reference solution was measured to be 7.6. Test substance was added directly to the
Tteessttvveesssseellssa:nNdowtagsivaepnp.arently miscible with water.
Number of concentrations: five plus 1 reference substance and 2
Tbleamnpkecroantturroles: 20-22 C
-
Total suspended solids and pH on day of testing: 3.6 g/L (TSS),
initial pH 7.3 and final pH 7.3
Element Basis: Respiration inhibition as determined by oxygen
consumption.
001755
Remarks: Values corrected to 20 C for calculations.
RESULTS
Nominal concentrations: 2 Blank controls, reference substance, 100, 180, 320, 560, and 1000 mg/L
Element values: 30-minute ECs = >1000 mg/L.
3-hour ECs = >1000 mg/L.
Element values based on nominal concentrations.
Remarks: Substance
Testing was conducted on Remarks field. The values
a mixture reported
as described inthe Test applytothat mixture and
not
the fluorochermical proportion alone.
z
CONCLUSIONS
The FC-206CE 3-hour ECs for activated sludge respiration inhibition was
determined to be >1000 mg/L.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY
Reliability: Kiimisch ranking 2. Testing meets all criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmationofthe amountoffluorochemical proportion in the solution.
REFERENCES
This study was conducted by the 3M Company, Environmental Laboratory, St Paul, MN, Lab Request number G1358-2, 1989.
OsTsHEsR--,seeseeeeeeeeeeeeeeeemeeeseeeeee:
Last changed: 6/26/00
001756
ENVIRONMENTAL LABORATORY
ACTIVATED SLUDGE MIXED LIQUOR
Date_-33-29
natyer SHR
Type Test_O ZC Acdyvated S| slag Respiat on Thi bition Test#Aoy
sourceMetco Unste Treatmen: Plant st Pavl, M
Date Obtained 4-23-89
Date Used (<2 3-2
Mixed Liquor Suspended Solids (MLSS) 3.
Lni =2.g 3 i Finaa l: 7l .3
Comments_TJest (on
als LR
17250
g/L Adjustment Nong BIL
a
Test Substance
Sample Description FC - 306 CE Lot SOR
sesple i ljghes (ore, Joconyi: 7.2adjuscaent Mone Comments
Reference Substance
Sample Description 3, 45> Dich lovopheno
sample pH_Z 4 [Stock <oln.) Adjustsent_Asone coments,
Exposure Conditions
_.
Test Duratton_3- Hours,
Test Temperature 20 22 %
Comments,
" -_--
--T
001757
.
Lab Request No. 1& 358-8
Sample Description
C-20bLE Lod 502 |
Yellow Ligprd
Environmental Lab -- Worksheet
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
.
.
P9 --L-- of --3
pate: __b~ A3-89
Analyst: A
a A, /
meme
Test Conc. mg added
BA
ee e e ee ee
ml Pf-water ml syn. sewage mlmicrobial . Total volume
Tas -
=
+
leo
S50 + 2s4 + Ib
180 do + 484 + lb
320 Jeo + A434 + Ib
0
0 + 48 + Ib
1000 Sop rv A84 Ib
+ ae
+ Aoo
+ Hoo + Aco
+ 400
+ op
> Soo
= Soo
= S500
= Soo
>
00
0: Soo
Blank Control TLshaved.with LAC 1750, a5 Blank Candral ]
3),
Twisial pik bh gest Canc testal, loco ma [Lz 7.2
oy
plt= 6. Oo
2 Pa pated
l- [2-89
cecahon Shared b-20-2q
001758 -
Gi358-
ENVIRONMENTAL LABORATORY
*
:
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
P8243
RAW DATA
Test substance: FC = ADLCE lot S02 Source of Activated Sludge MixedLiquor: Metro UJaste Treaiment PlantSiR,
Date Receiyed: b- 23,
----
vi --Boacd
Date Used: 4-23-89
BEST COPY AVAILABLE
30 - MINUTE EXPOSURE
ConceesnttratSiubosnta(ncseg)
DISSOLVED OXYGEN CONCENTRATIONS ma/l HE-HTNU
ApCONTROL I 00 20 60
como
[24]7.26.6 [6.1 [5.651 [4.5140(3.513.0|2.5]
[21[78]2.3).0le3|5[81.]31s838 1l5.43a| 2.217.5170]64 [5.9]5.a)4.2[94]5.7]3.2]2.7],
[7417.1les|[1.5 5138805 ]|[25 511-.81112]
Zalz[i slsl0le islu ioblsse .oBl3]
3 - HOUR EXPOSURE
CELA conteesnttratsuibosntalnaceg/L)
DISSOLVED OXYGEN CONCENTRATIONS mg WE-MINUTES
contho 1
[2.617.201[44.6.]4.71[325]53.00]2.]51
0
l8.0]7.4]7.5]2.\|8e5.47]5]0kl4..2b]145.1.|
120
12.3[247..0 |1Lb|[na7ls.s[7551]5.71[1..7]
550 18.315.112.87.512.\[p2lb4lL.0]57[5344|
coumso 11 [73[71lesleols slsolsy[34153139]23]
REI SS PH of Activated SludgeMixed Liquor: jig 7.3
ee Sins 7.3
Test Substance
ENVIRONMENTAL LABORATORY
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
CALCULATION OF RESULTS
EC. =
Lot 50
wR GI359-. eld
ys
ra3e43
wa, 30 - MINUTE EXPOSURE Test Substance
CONTRO Joo 80 20 60 000
En n Uptake Rate
R Coryected' 1)
%
Inhiibhiietdion
1 in
| o.saon [ 0.5000 | 0.5222 | 0.6000 |0bn/2 | o.79ex
[oH474a | -- [04776 | = [os3285 | = To573 | -- loesal | = [o.7127 [| ~
CONTRO
0.5787 Tower7
Percent Difference in Controls)__ /. 5%
ECgy (95% Confidence Linits) mg/L
L
dR EECSR
|=
ConTceesnttraStuibosntan(cneg/L)
--
00 130 330
20 1000
jen Uptake Rate
R Corrected 1]
4% IRnehsipTobidirtait43, ioonniRnate(?)
0.525:
loza| y --
|0.4aoo0 Todaeo |
| 0.3776 [03770, | 2
"| 0 toso 10Hoop |
| 6.3576 [0.3576 | 24
| 0.3945 [oye | 12
CONTRO
lo.s2al [o.4gag |
Percent Difference in Controls7%)__0. :
Bog (95% Confidence Limits) mg/L--21000MIL
@) Tew. Corrections LBL (ng 0,/L-nin.)
o
RG, + iC
@ Inibitions
4. x X 100
Where:
(er7m)
TR,o=bOsxy=geSnamupplteakteemrpa.,teC*(n,g O/L-min.)
..
6=1.047, "x = T,0bs - 20C
C = Control
ustorence stLA 100
RC, +
&; = Tshheoutlwdobceonwtirtohlirne1s5p% iroaftieaocnhraottehse
See pad for test soln.
Prep.
.
001760
Pom1063.8.0- m0
Sample Description
Z4o5f,erDeincchel.oicphennoel
sanch Red. Labol, Lot
tecpie crystals
&1358-R
Lab RequestNo. 1& 250
Environmental L--aWobrk Sheet "0 --L ot _3
RESPIOREACTIDOANCTIINVAAITBEITDIOSNLUTDEGSET 4209
Date: b-23R81
Anais: SAR
Stock and Test Solutions Preparation
STOCKSOWITION: 500 my 3,6-DCP J
fad. Jomd = 0:5 ge
~Dissolued Seome 3,5: DP in Jom jou Auoh
"Dilvtetdo 3p ml wiMelL-Q wake
.
------ "ADdidluetdedenddoeraSnhSheii.tnliigtae,rLwoitmteHMaiklolyiA-Qsoopduiesre..untiill cineient.FP
Sack Solu pH 2.6
-_--
TestConc, mlstocksoln mI DfwatemrI syn.sewage Al Rcrobial
ng/L Sr S00 ml
Ylang DOT
RerS00m feed per S00 ml inoculum per, S00 m1
231 +
16 + Aco
=
10
lo
Az4 +
7A
Aco
=
-_--
Total volime
nl
Soo
Soo
"Pc)onttols shared wih, 10Gaizse
-_-- _-- -_-- -_
-_-- ---
TE
Milli-Q water used 2 Prepared le-19-84_
000000000
a@cadion Start) -20-29
001761
TT
GLI
ENVIRONMENTAL LABORATORY
*
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
RAW DATA
PaA $3
Test substance: _3..5-Dichlorophenal Reference,Substance
Source of Activated Sludge Mixed Liquor:Metrobaste TreatmentPlantStRaulav
Date Received: (p-23-89
Date Used: (-23-89
Analyst: Lo
a. Road,
BEST COPY AVAILABLE
30 - MINUTE EXPOSURE ST substance
DISSOLVEODXYGEN CONCENTRATIOaN0S
ME-MINU
TEM
CONTROL I
Io
CONTROL 11
Fe 5]
5
2
7472069 bles bols750151 4.214.502
I
E rrH r rI rr
Sirs bgloalsa site 5]Bsl3ol.y] 2c
Contentratton mg/L) 3 - HOUR EXPOSURE [est substance
conmaoL1
DISSOLVED OXYGEN CONCENTRATIONS mg WE-MINU]
TEL
[7.3120[0.5[pols col44a]3:3]a4[oa a
contaoL 11
HH Cerrar rrr
rrr
rrr
rrrrrr
[7407.1[bbb[551p55040241[25.0 Jaz
ppHoof hAicgthievasttecdonScleundtgreatMiioxnedteLsitqeudo:r: P Inil tiaal n7e5
Honsal) 77..59" 0017a62
Ta oD aT Fo oo
VIHUNENIAL LABORATORY
ACTIVATED SLUDGE RESPI!
INHIBITION TEST #209
Testsubstance2.5 D IchlnraphenalRefeconte Subglance
Relzse
Date fy
Pa3ep3
30 - MINUTE EXPOSURE
ConTceesnttrSautbisotnan(cneg/L) CONTRO
Oxygen UpRtake Rate RrtoCo20rpCected
+ IRnehsipbiirattiioonniRnate(?)
| 0.5127 |o.4677 | =
| 3000 |.2737|
1
Tr
TT
CONTRO
|0593 louqsz |
etPercent Difference in Controls()
ECgy (95% Confidence Linits) mg/L
l Foon
Test Subst:
LZ
4
R Co ted 3,
.0
% EE ho Inhibition i
ONTRQ
0.529
o 484
=
|0.2 | 01 .2055 5 |8 5
|
1
1
11
1"
CONTRO
[0.5855 |o.4794 | =
Percent Difference in Controls)__ 0.7% . y
ECgg (95% Confidence Lints) uo malin) 57 % InR hibie lon
(1) Temp.. Correction:= LEE (ng 0y/1o~min.min.)
+ DigFeerences 2 ddS]d ae
@ V Thtbistonsier a
X10
. shhoeultduobeconwtrol.i1re1s5tp0orFehspieraatiiconhroattnehse'
See. pa4 Lor Stuck +
WRh=erCe:ynwpa ratefn 0y/onin)
test sel. prep
800 ,0bs =
hnys Caei
BEST COPY AVAILABLE
= Control
001763