Document Z49vMZgp4wvnjgVjZG2j3Lpa8
/IR -0 3 S 5
ACTIVATED SLUDGE RESPIRATION INHIBITION TEST
TEST SUBSTANCE
Identity: Perfluorodecanesulfonate, ammonium salt; may also be referred to as PFDS, FC-120, or 3M Sample J2905-1. (1-Decanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10heneicosafluoro-, ammonium salt, CAS # 67906-42-7)
Remarks: Test sample is a mixture of the test substance in water and butyl cellosolve (approximately 25% test substance, 37.5% butyl cellosolve and 37.5% water). All values reported relate to this mixture. No calculations were made to adjust for the actual concentration of the test substance in the test sample. Test sample was from 3M Lot 164.
METHOD
Method: OECD 209 Test type: Static acute GLP: No Inoculum source: Metro Waste Treatment Plant, St Paul, MN. Inoculum holding: None - used the day it was obtained. Inoculum MLSS: 4.3 g/L. Year Completed: 1992 Analytical monitoring: Dissolved oxygen concentrations. Exposure Period: 30-minutes and 3-hours Measurements: Dissolved oxygen depletion rate after 30-minutes and 3hours of exposure.
RESULTS
Nominal concentrations: Blank controls and 1000 mg/L test material solution, in duplicate.
Element value: 30-min and 3-hour EC50 > 1000 mg/L
All element values based on nominal concentrations.
Inhibition in respiration rate: After 3-hours exposure, the mean of both replicates was 17.3% inhibition at 1000 mg/L
Control response: The largest percent difference between control uptake rates was 1.5%; thus the two controls were within 15% of each other.
000153
Reference substance results: 3,5-dichlorophenol at a concentration of 10 mg/L induced 57% inhibition in respiration rate. This was within the normal range seen in this laboratory with this inoculum. Remarks: Testing was conducted on the "as-sold" mixture of the subject material (25% subject substance in water). The value reported applies to that mixture and not the test material. CONCLUSIONS FC-120 exhibited a 17.3% inhibitory effect at 1000 mg/L after a 3-hour exposure. Testing was conducted on the "as-sold" mixture of the subject material in water (-25% subject material). The values reported apply to that mixture and not directly to the subject material.
Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking 2. This study, while well conducted, lacks analytical data for: determination of the test substance concentration in the test solutions: and determination of the sample purity. Because a mixture was tested, care must be taken when interpreting the results. The solvent in the mixture could have enhanced or restricted biological uptake and/or toxicity of the fluorochemical component. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, St Paul, MN, Lab Request number J2904, 3/6/92. OTHER Last changed: 5/18/00
000154
ENVIRONMENTAL LABORATORY
ACTIVATED SLUDGE MIXED UQUOR
LR J S W j H Date 'b h /1 2
Analyst
Page J __ of _
Type Test Q B c . 0 f ic p T tQ Su/OGL RlSPift'VnO^ ~X^H'P)tT\or> T * s t
ACTIVATED SLUDGE MIXED LIQUOR
Date Obtained ~ b ib f1
Date Used
Mixed Liquor Suspended Solids (MLSS) 4 -? ) g/L Adjusted to ________ g/L
MLSS in final test solution
g/L
Initial pH
(1st test soln. prepared)
Enai pH
7 (last soln. prepared)
Comments ______________________________________________________________
T5ST..3UBSTANC5
Sample . Description
l^Q T
_________________ _ _ _ _ _ _ _ _
initial pH of highest cone, tested ( \OCC A-) - ~7-
Adjusted to pH ______
j%
Comments _______ iP-iou L>q^> o __________________________________
REFERENCE SUBSTANCE
Sample Description
^ > l'b ~ O iC M V o R c p n ^ c i_______________________ __
Initiai pH of stock solution f STt*? mg/L) - *0 I
Adjusted to pH
i
I
Comments _________ ______________________________________________
EXPOSURE CONOmONS
Test Duration _
3
Test Temperature Range:
c
Comm ents _______________________________ ______________________________________
000155
ENVIRONMENTAL LABORATORY
LR I Z W ' I
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST S2C9 TEST SOLUTIONS PREPARATION
Test Substance: ___ 'Y C " Q O
L ot
Sample Description:
JjLkCLW__L
Analyst:
ESN
Date:
/Q
\ b'H
Test Cone. - ma/L
mg added Der 500 mL
mL MOO ) water Der 500 mL .
______ __________
mL syn. sewage feed oer 500 mL
. Jb
mL microbial inoculum oer 500 mL
3 c d ___
\QCO-A___ ____ <Q Q ____ ______ M ______
'U
900
irvDO-(S
S C O ____ d?A
_______ Ua_______
see
T ry it :.?1__ ` A : 7 -"7
(V 7-7
Total Volume mL
<00
<oo
<oc?
(1) Millipore Milli-Q^ high purity water
000156
P..J S : -1
ENVIRONMENTAL LABORATORY
LH ^ 0 4
CECO ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209
RAW DATA
Test Substance:
VC -Id O
L O T 1 1 ^ _______________________________________
Source of Activated Sludge Mixed Liquor: 4'V tq.j
f C f- 9ry^>. t
Date Received:. 3 / b / q >
Date Used:
Analyst:
CSN\______________
30-MtNUTE EXPOSURE
Test Substance Concentration
) 0.5
1.0
BK CONTROL!
%A 3 . 0
DISSOLVED OXYGEN CONCENTRATIONS. mgrL
TIME- MINUT ES 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0
7 .G 7.<? G3- G G. 1
7 3 4 -7 4.;?
Temp 5.5 C
3.7 PS.6>
\O C C - . . ino.c-fe
^ .0
3.1 3.
3,1 1.Co 1.0 0 - 7 --
-- . 910
M l *lc?
0 .^ -- --
----
-- - ---- -
BKCONTROL U
"7< j 7 1
Cp- 3 -7 S '-7 4 .3 4 7
6.7 3-1 7 7 <?c.<5
3-HOUR EXPOSURE
DISSOLVED OXYGEN CONCENTRATIONS, mgL
Test Substance
TIME MINUTES
Temp
Concentration (*^Jl ) 0.5 1.0 1.5 2.0 2.5 3.*0 3.5 4.0 4.5 5.0 5.5
C
BK CONTROL 1
S/3 7.<r 7.0 G-4 GsO S'.S' vO 4 .r 3-7 3.1 7.4
1003 -1)
15-3 7-7 >5- 7. | C.7
5-7
4-\ 30.5
\ncv. -> ?*,*> *hfl 7.G>
U3> s'. i <7-4 5-1
3K CONTROL II
7.1 G.7 G.3 s.Ss S3 4.3 4-7 37 33 <775 7-4
pH of activated sludge mixed licucr: initial ~7.S~_______
pH of hignest concentration testec:
initial ~7. C-._______
Test temcerature:
\ ct.k '~ ^ I.Q X
000.157
Final 7 -7 F:nai ~).S
)\.io
ENVIRONMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 CALCULATION OF RESULTS
Test Substance: FC-120 LOT 164
Analyst: KSN
P., 4 ^ 4 ,
Lr: J29Q4-1 )ate: 3 /6 /9 2
mq/L 0.5 v %w ' '
BK1 8.4 1000.00 4 .5 1000.00 2.8
1.0
8.0 4.0 2.2
1.5 --- **
7.6 3.6 1.6
2.0
7.2 3.1 1.0
2.5 s'
6.6 2.6
-
3.0 k'Vv-k
6.1 2.1
-
3.5 1
5.6 1.6
4.0 :k
5.2 1.0
-
4 .5 5 .0 5 .5 Temp, *C
. -P k :-vfe
..V
4.7 4.2 3.7
20.0
- - - 21.0
- - - 20.7
R (D Ra (2) % Inh. (3)
0.9661 1.0000 1.2000
0.9661 0.9328 1.1430
0.0 3.8
0001
BK2 7.6 7.1 % dilf in controls (4):
6.6 0.7
6.2
i / I 3-Hour Exposure
00 mg/L 0.5 1.0
BK1 1000.00 1000.00
8.0 8.3 8.5
7.5 7.9 8.0
1.5 .>
7.0 7.5 7.6
2.0
6.4 7.1 7.2
5.7 5.2 4.9 4.2 3.7 EC50 (95% Cl): >1000 mo/L
3.1
2.7
Dissolved Oxygen Concentrations, mq/L 2.5 3.0 3.5 4.0 4.5 5.0 5.5
6.0 5.5 5.0 4.5 3.9 3.4 2.9 6.7 6.3 5.8 5.3 4.9 4.5 4.1 6.8 6.3 5.9 5.5 5.1 4.6 4.2
20.2
0.9867 0.9730
-
RaCm (5): 0 .9 696
Temp. *C
R (1) Ra (2) % Inh. (3)
19.9 20.5 19.9
1.0194 0.8594 0.8485
1.0265 0.8300 0.8544
18.5 16.1
BK2 7.1 6 .7 6.3 5.8 5.3 4.8 4 .2 3 .7 3.3 2.8 2.4
% diff in controls (4): 1.5
EC 50 (95% Cl): >1000 ma/L
19.6
0.9830 1.0108
RaCm (5): 1.0186
(1) R . Oxygen uptake rale (mg 02/l-m ki) (2) Ra- R adjusted to 20*C- |R/e.Ex) (3) % Inhibition |(RaCm)-RaS/(RaCm)]10Q (4) % differone* in control: (IRa8k1Ra8k2VRaCm]100 (Two controls must be within 15% of each other.) (5) Mean adjusted R, controls- (RaBk1RaBh2)r2
e - 1072 RaCm- mean adjusted R, controls BK- blank control, S - sample T o t*- sample temp x Tobe-20*C
ENVIRNOMENTAL LABORATORY
lr_ i ^ 21.
OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST # 2 0 9 TEST SOLUTIONS PREPARATION
Test Substance: Sample Description:
ft
QtCHlpHOPHfcMCL ^ CM ^ vC - t S ^ S T . W - j
__ ^ to Lg/br
lot P'7 , o yO -Q
Analyst:
____
Date:
' j b / 9
Stock Solution
Preparat_io_n:__________5_C_0__enc^ / 1_ ____ iftC H ^-0
- Q5" /V1^-
P'MeurfD <?QC
^ - O C P ^ 10
LON
0
ftrooi0_* 90
- .0
fVnoCo 1.0 V4 yA. SJ,. nt^Pw 155___ ryi'l
'/"TIL__1~ >1f\ t ~T__PPT.
AnnCO ~ S"C nv*__~n.. L.1- _Q_j C it ..__ ^Tcic
__ i^ ;ri^ oH - _?. 1
ftTki r r to TC__T O __ >-nt___A jSOi, # T V ^ T C D TO 1 U)T< L.i'f'n
/Vivi l~Cl
____ 3TCC.I*- <bci-*T>0~ pH * 1 . 7
Test Cone. _moZL__
lC Ccmthcl
mL added Der 500 mL
mL MQ<1) water mL syn. sewage per 500 ml__ feed per 500 mL
2S4
Uo
mL microbial inoculum Der 500 mL
300
Total Volume mL
TOC
O
1 0 ____
___________ 2_________ ______ a e c ________ i C C
i
(1) MiMIpore MII-QR high purity water
000159
V. ' -
ENVIRONMENTAL LABCRATCRY
u.Xic'i
L.=n T 3T-3Q
CEC3 ACTIVATED SLUCGc RE3PIRATICN INHIEITICN TEST #209
RAW DATA
Test Substance:
, *1 ~ T~~y-, c ,-; r.V -cP hf^G 1-
Source of Activated Sludge Mixed Liquor:
Date Received: ~b f 1 ^ 3
Analyst:
______________ _
W q'i-^nNT R a-"; Date Used: ^ / k / C'>c)
30-MINUTE EXPOSURE
Test Substance Concentration ( r ^ /c ) 0 .5
1.0
DISSOLVED OXYGEN CONCENTRATIONS. mg^L
TIME MINU7 E5 1.5 2.0 2.5 3.0 3.5 4 .0 4.5 5.0
Temp 5 .5 C
BKCCNTRCL1
fiA S o
*7.> Gr -C-. V
"T, '<J < T .*
N^ &C-.G
\0
1 1.*!
~7.1 (o ,c> Pi-6 ' O-j
t .d
EK CONTROL U
_7 ^
i *\Q
^l
(- b . 2 b .7 if-d
i . > 7 ^ .l 7 30..?
3-HOUR EXPOSURE
DISSOLVED OXYGEN CONCENTRATIONS, mgrt.
Test Substance
TIME MINUTE3
Temp
Concentration ( - ^ / l ) 0 .5 1.0 1 .5 2 .0 2 .5 3 . 0< 3 .5 4 .0 4 .5 5 .0 5 .5
=0
EK CO NTRO . I
<5-0 I S "7.0
I
'O W h 7 -P i-i I
!
I
______________._____ i!______
SK CONTROL :l
~). I
I Co.7
G . 0 < T -f S s U " i - c/ I A
I
V?
^ -5
V i 7 .3
II
I
lili
! __
I II!
I Itil
^ *>
^ 3 A S 4 .3
d . I D .O
OH r ac:;vatec s.'ucge mixec icucr: OH r -ig n e st ccncar.rrancn 'esrec:
"es tem perature:
\.U - IXVis
'nitiai 7.^_____ mtiai l . ' o
nai ? -7 n ai A
000160
9 - cl I t i I II I
i
9 .4
K .9 M . Oj
ENVIRONMENTAL LABORATORY OECD ACTIVATED SLUDGE RESPIRATION INHIBITION TEST #209 CALCULATION OF RESULTS
Test Substance: 3.5 DICHLOROPHENOL
Analyst: KSN
} ci ?>
Lr: ,)?90? & J29CWUiiJO Date: 3 / 6 I 3 9.
mq/L 0.5
1.0
1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 Temp. "C
R (D
Ra (2) % In h . (3 )
BK1 8.4 8.0 7.6 7.2 6.6 6.1 5.6 5.2 4.7 4.2 3.7 10.00 8.1 7.9 7.5 7.1 6.9 6.6 6.3 5.9 5.6 5.2 4.9
20.0 20.8
0.9661 0.9661 0.6533 0.6180
-
36.3
0001G 1
BK2 7.6 7.1 6.6 6.2 5.7 5.2 4.9 4.2 3.7 3.1 2.7
% dill in controls (4): 0.7
EC50 (95% Cl):
20.2
0.9867 0.9730 RaCm (5): 0.9696
3-Hour Exposure
Dissolved Oxygen Concentrations, mq/L
mq/L 0.5
1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 Temp, *C >:<
R (D Ra (2) % Inh. (3)
BK1 8.0 7.5 7.0 6.4 6.0 5.5 5.0 4.5 3.9 3.4 2.9
19.9
1.0194 1.0265
10.00 9.4 9.2 9.1 9.0 8.8 8.5 8.2 8.1 7.8 7.6 7.4
19.6 0.4255 0.4375 57.1
BK2 7.1 6.7 6.3 5.8 5.3 4.8 4.2 3.7 3.3 2.8 2.4
% dill in controls (4): 1.5
EC 50 (95% Cl):
19.6
0.9830 1.0108
-
RaCm (5): 1.0186
(1) R- Oxygen uptake rate (mg 02/l*min) (2) Ra* R adjusted lo 20"C (R/e.ExJ (3) % Inhibition* |(RaCrn)-RaS/(RaCm))U)0 (4) % ditlerunc in controls: {lRaBM-RaBk2URaCm)t00 (Two controls must be within 1$% ot each other ) (5) Mean adjusted R. controls* (RaBkt RaBk2K2
* 1072 RaCm* mean adjusted R, controls Bk* blank control. S= sample Tubs* sample temp x* Tobs-20*C
