Document Z45NgwXGNnaM1z19kjn95QDq0

Aft _ 0 6 3 4 TOXICITY TO AQUATIC PLANTS TEST SUBSTANCE________________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95 or as a component of FC-201AF. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FC-201AF. Current information indicates it is a mixture of 3% PFOS, 18.59% diethylene glycol butyl ether, 37.81% water, 14% ethylene glycol, 8.25% Acrylic foamer, 9% sodium octyl sulfate, 0.3% sodium lauryl sulfate, 3% triethanolamine, 6% sullfonated alkyl ether salts, and 0.05% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect f/ie toxicity o f the fluorochemical component o f the test sample. METHOD:_________________________________________________________ Method: OECD Guideline 201 and EPA CFR 40 797.1050 Type: Acute Static GLP: No Year study performed: 1995 Species: Selenastrum capricomutum Source: In-house cultures. Originally from ATCC, Rockville, MD Element basis: Algal cell counts (cells/ml), specific growth rates. Exposure period: 96 hours Statistical Methods: Not applicable due to study results. Analytical monitoring: Not given Test Conditions: Algal Nutrient Medium: Nutrient medium for freshwater algae per OECD method. This medium served as the diluent for all algal operations. The pH of this synthetic algal medium was 7.9. Stock and test solution preparation: A 1000 mg/L primary stock solution was prepared by diluting 500 mg of test substance in 500 ml_ of standard algal netrient medium. Aliquots were then added directly to dilution water in test vessels to create test solutions. Exposure vessels: Not given. Agitation: Not given. Number of replicates: Not given. Initial cell loading: 1 x 104 cells/mL Number of concentrations: One, plus a blank control Lighting: Continuous Water Chemistry: Not given. 000191 RESULTS Nominal concentrations: Blank control, 1000 mg/L 3 Algal Growth Response EC50 values Exposure (Contact) Hours 24 48 72 96 Mean Percent Change in Specific Growth Rate (1000 mg/L Cone.) 13 14 22 18 EC50 (mg/L) >1000 >1000 >1000 >1000 Element values are based on nominal concentrations. Remarks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical components alone. C O N C L U S IO N S __________________________________________________ The test substance exhibits a 96-hour EC50 for specific growth rate of >1000 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY _________________________________________________ Reliability: Klimisch ranking = 4. Not all laboratory data could be located. There is no characterization of the test substance purity, analytical measurement of the test concentrations, and confirmation of the amount of fluorochemical components in the solution. REFERENCES___________________________________________________ This test was conducted by the 3M Environmental Laboratory, St. Paul, MN, Lab Request number N3281, 1995. O T H E R __________________________________________________________ Last changed: 6/26/00 000192 0 6 /2 5 /2 0 0 0 ENVIRONMENTAL LABORATORY ARCHIVED F IN A L REPORT 1 LAB REQUEST NO. N3281 REQUESTOR NAME: SBS/SHARI LOUSHIN DEPARTMENT: 3058 PROJECT NO: BILTWENVIR DATE RECEIVED: 09/28/1995 DESC: FC-201AF LIGHT WATER CONTRACT LAB(S): EXP COMP DATE: 12/04/1995 DATE COMPLETED: 01/17/1996 PROJECT LEAD: TL HATFIELD PHONE NO: 651-778-7863 3M FAX NO: 651-778-6176 Results for toxicity to fish, Daphnia, Microtox and activated sludge resp. can be found on lab request N2964 sab 10/3/95 Standard testing for AFFF products. Formulation is on Doc. # 07-2573-9 in PRISM. 3M ID number given as ZF-0002-0843-7. sab 10/3/95 Results from algae toxicity test: Test Cone. mg/L 1000 Mean Percent change in Specific Growth Rate 24-hrs 48-hrs 72-hrs 96-hrs 13 14 22 18 EC50 sab 1/16/96 >1000 >1000 >1000 >1000 SAMPLE DATE CODE DESCRIPTION RESULT 1 09/04/95 FC-201AF IC50-ALGAE ALGAE ASSAY Dark Liquid IC50 >1000 MG/L MDL or (95% C.I.) (-) 000193 b e s t c o p y a v a il a b l e ENVIRONMENTAL LABORATORY PREPARATION WORKSHEET FOR FRESHWATER ALGA GROWTH INHIBITION TEST0' LR SEE BELOW PAGE OF date to A f t s ANYS^^-S __VC. LotYA'P' L o * *SQ\__LTdVrl .amlvv U i'vnu& ^ o tu M g . >ri\xi^-W~_________ .S n O m g / ^ D D w b . l ^ f l . -Al/V^l N iL c trirP n - M f l l 'i u m . _____________________ ___________ IrtM LaltjlH *1,9._______________________________________________________ Lii^h-i ujAfgy*^VAi>vU^FFF. C\rav~ ftotharcoXfiraK A ltniid. __ y y ^ n /^ y n iv tL > ^ fk A . K lg a L M A V I g n f : X o d k o M ____________________________ ____________\iAi-fi'<^r.^V4 *1.3-----------------------;----------------------------------------------------- --------------- _ PA ( L -tM Z fi) '?Vn4ir<4T\;g P HgvnLfa.1. 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Iy r 4?vtW * 1 r U i i < o M rv 1 4 u v y v > U t ?4- u O ^ S 4 i t * 1 v^ a f t gir U ite fa l j - ' x.trfi ;'-i:' * ^ ^ t \t u aJ* B vc rrvvWol t e i r t V 'I x ^Test'methodotow conforms with the foltowing two lest protocols:^^ iOECD #201 "Alga."Growth Inhibition Test* and Fed, Reg?Vol. 40/No:797.1050>`AlqarAcut^ 000194 BEST COPY AVAILABLE ENVIRONMENTAL LABORATORY STANDARD NUTRIENT MEDIUM FOR FRESHWATER ALGAE NUTRIENT CONCENTRATION IN STOCK SOLUTION FINAL CONCENTRATION IN TEST SOLUTION STOCK SOLUTION 1: MACRONUTRIENTS n h 4ci MgCIi 6H20 CaClj 2Hj0 MgSO ` 7H20 KH2P04 1.5 g/L 1.2 g/L 1.8 g/L 1.5 g/L 0.16 g/L 15 mg/L 12 mg/L 18 mg/L 15 mg/L 1.6 mg/L STOCK SOLUTION 2: Fe-EDTA FeClj 6H20 Na2E D T A 2 H 20 80 mg/L 100 mg/L 80 ug/L 100 ug/L STOCK SOLUTION 3: TRACE ELEMENTS HjBO, MnC^ 4H20 ZnCI2 CoCIa 6H20 CuCIa ' 2H20 N aM o O i^H jO 185 mg/L 415 mg/L 3 mg/L 1.5 mg/L 0.01 mg/L 7 mg/L 185 ug/L 415 ug/L 3 ug/L 1.5 ug*L 0.01 ug/L 7 ug/L STOCK SOLUTION 4: NaHC03 NaHCOj 50 g/L 50 mg/L Sterilize stock solutions 1-4 by membrane filtration (0.2 urn). LR M1655 N3188 N3194 N3281 000195 96T000 ENVIRONMENTAL LABORATORY V>-.HWNg FRESHWATER ALGA GROWTH INHIBITION TEST SPECIFIC GROWTH RATE ALGAL SPECIES: Freshwater Green Algae, Selenastrum capricomutum DATE INITIATED: Ia / q / q; | u a a DATE TERMINATED: |0 / | ^ , T U,,nO ' TEST SUBSTANCE CONCENTRATION mg/L 24 HRS 48 HRS 72 HRS:,'. v MEAN OR RATE % C H A N G E * MEAN OR RATE % CHANGE " MEAN OR RATE % C H A N G E" BESI BK CONTROL STD NUTRIENT 2.104 I.U 48 1.444 M1655 1000 mg/L 5.5 1.2-8Z, 2.2- I.M *. N3188 1000 mg/L 1.4RZ- 15 1.498 <U 1.2.5 N3194 1000 mg/L 0.551 15 O.SR4 (4 0.580 N3281 1000 mg/L 1.841 15 1.4H 14 U S 5 DATE/ ANALYST IQ/il Test methodology conforms with the following two test p r o to e o te y ^ ^ OECD *201 *Alga, Growth Inhibition Tesr and Fed. Reg Vol. 40. No. 797.1050, 'Algal Acute Toxicity Test*. Specific Growth Rate (u) - InN. InN^t. Where N, is the mean measured final cell density; N, Is the Initial cell density and t Is the time of the measurement. TMPercent Change in Growth Rale % - (Mean Control Value Moan Sample Value)/Mean Control Value x 100.