Document Z43kDw6XeZN78r9wekD0mkYNL
A R A 2.6 - 085
ACUTE TO XIC ITY TO AQUATIC INVERTEBRATES (DAPHNIA MAGNA)
TEST SUBSTANCE_______________________________________________
Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-603F. (1Octanesulfonic acid) (CAS # 2795-39-3).
Rem arks: The 3M production lot number was 501. The test sample is FC603F. Current information indicates it is a mixture of 0.5% PFOS, 7% diethylene glycol butyl ether, 82.95% water, 2% acrylic foamer, 4% Alkylether sulfate, 1.45% Xanthan gum, 1.4% calcium acetate, 0.07% N-(3Chloroallyl)hexaminium chloride, 0.5% triethanolamine, 0.05% sodium hydroxide, 0.03% cis-1 -(3-Chloroallyl)-3,5,7-triaza-1 -azoniaadamantane chloride, and 0.05% tolytriazole.
The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f the test sample.
METHOD:______________ _________________________________________
M ethod: OECD Guideline 202. Type: Acute static GLP: No Y ear com pleted: 1994 Species: Daphnia magna S u p p lie r In-house cultures. Originally obtained from U.S. EPA-NETAC, Duluth, MN. A nalytical m onitoring: Temperature, pH, and dissolved oxygen. Exposure period: 48-hours Test organism age: <24 hours old S tatistical m ethods: Not applicable. T est conditions:
Dilution w ater: Carbon-filtered well water (3M Well #2, St. Paul, MN). Dilution w ater chem istry: Not given.
Lighting: Not given. Stock and test solution preparation: A primary stock solution of 2000 mg/L was prepared, diluted to 200 mL and divided into 4 -5 0 mL replicates. Aliquots of the primary stock solution were used to prepare the remaining concentrations. Exposure vessels: 100 mL vessels containing 50 mL exposure solution. Num ber o f replicates: 4 Num ber o f daphnids per replicate: 5 Num ber o f concentrations: five plus a blank control W ater chem istry during the study:
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pH range (0-48 hours): 8.4 - 8.4 (control exposure) 8.4 - 8.4 (1000 mg/L exposure)
Tem perature range (0-48 hours): 20 - 22 C Dissolved oxygen range (0-48 hours):
8.3 - 8.3 mg/L (control exposure) 6.4 - 8.3 mg/L (1000 mg/L exposure)
RESULTS _________ ___________________________________________
Nom inal concentrations: Blank control, 100,180, 320, 560, and 1000 mg/L.
Elem ent values: 48-hour EC50 = >1000 mg/L
Element values based on nominal concentrations.
Rem arks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone.
C O N C L U S IO N S ___________________________________________________
The FC-603F 48-hour EC50 was determined to be >1000 mg/L, the highest concentration tested. No mortality or sublethal effects were observed.
Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133
DATA QUALITY___________________________________________________
Reliability: Klimisch ranking = 2. This study meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution.
REFERENCES____________________________________________________
This study was conducted by the 3M Company, Environmental Laboratory, St. Paul, MN, Lab Request number M 1 6 5 5 ,1994.
OTHER______________________________________________________ _
Last changed: 6/26/00.
002460
3M ENVIRONMENTAL LABORATORY
LR M l 655
1 Date: 5 /2 7 /9 4
;
Analyst:
JBP
Daphnia magna
; 48-hour Toxicity Test Data Summary I
j SAMPLE: FC-603F (Lot #501)
ii
Sample Cone. No. Organisms
(mg/L)
Exposed
100 20
180 20
320 20
560 20
1000
20
% Immobilized
24 hours
48 hours
00
00
00
00
00
24 h EC50 (95% C.l.) = 48 h EC50 (95% C.l.) =
> 1000 mg/L > 1000 mg/L
EC50 Estimation Method: N/A
b e s t c o p y a v a ila b le
BEST COPY AVAILABLE
ENVIRONMENTAL LABORATORY 48-HR ACUTE TOXICITY TO DAPHNIA, OAPHNtA UAGNA
mifesy
TYPE TEST:
STATIC
TEST SUBSTANCE:
____STATIC RENEWAL ___________
( LcA * S cO s
DILUTION WATER:
CARBON-FILTERED WELL WATER (3M WELL . ST. PAUL. MN
TEMP. =>* -c ,
pH 8 h D.O. K .3 mg/L
TEST DAPHNID SOURCE: U.S.EPA-NETAC, Duluth. Mn _________ DATE RECEIVED:
^ ~ q .C
DAPHNID AGE: <24 HR OLD NEONATES
NO. OF DAPHNIDS/TE5T CHAMBER:
_____5
LOADING RATE: *
U .TEST SOLUTION VOLUME: 50 mL SIZE OF TEST VESSELS:
100 mL
DATE INITIATED: <T- iX S -
DATE TERMINATED:
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m g /L ADDED*
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