Document Yv40QJo4QZGK7nGKNNq45eRV

I 329 BUSHY RUN RESEARCH CENTER * D- 4- Mtlten Road. Export, Pennsylvania 19832 Talaphona (412) 327-1020 UCC BUSINESS CONFIDENTIAL: Not to be released outside UCC without the written consent of the UCC-sponaoring Division HS&EA Manager. Project Report 44-50 4 Pages Tel: (412) 327-1020 May 12, 1981 BAKELITE Phenoxy Resin PKHH Range Finding Toxicity Studies Sponsor: Onion Carbide Corporation Sunaary Peroral Intubation, rat - LD50 >2.0 g/kg; 1 ml 0.025 g in 0.251 agar plus 0.10Z Tween 80. Percutaneous, rabbit - ID50 - 4.00 g/kg; 1 ml - 0.25 g in mineral oil. Uncovered Skin Irritation, rabbit - None from 25Z in mineral oil. Eye Injury, rabbit - Trace from solid; none from 25Z in mineral oil. Interpretation BAKEIITE Phenoxy Resin PKHH was no more than moderately toxic following single peroral Intubation and was moderately toxic following single percuta neous application. Administration of a 25Z suspension (poor) In mineral oil resulted in no irritation to rabbit skin or eyes. Instillation of 40 mg per eye of the solid particulates resulted in trace corneal Injury In 2 of 5 eyes. Sample Quantity: 1 lb Date Received: December 3, 1980 BRRC Sample No.: 43-379 Submitted By: R. A. Gregory Division: Coatings Materials B und Bro k, NJ Descrlptl n: coarse, sand-like solid ushy Run Roaasion Center Charge No.: 07233 A Joint Motion InatItuta--Union Carbide Corporation Operation . 0 030 Report 44-50 Page 2 Peroral, Single Dose to Rata LD50 2.0 g/kg; 1 ol 0.025 g auapeaalon In 0.25Z agar plue 0.10Z Tween 80. Conditions - Standard. See attached page of standard test procedures. The suspension In agar and TWeen 80 was the most suitable form to complete the dosing and 2.0 g/kg was the highest attainable dosage. Dosage; Dead/ Days to g/hg Dosed Death 2.0 0/6 Weight Change, g 83 to 125 (mean-103) Signs Yellow-brown discharge from genital area at 40 min. Gross Pathology - Nothing remarkable. Conclusions - No more than moderately toxic following acute peroral intubation. Percutaneous, Single Dose to Rabbits LD50 - 4.00 (1.12 to 14.3) g/kg; 1 ml - 0.25 g suspension (poor) In mineral oil. Conditions - Standard. Dosed under polyethylene sheeting. Dosage; Dead/ Days to Weight g/kg Dosed Death Change, g Skin Irritation Signs 4.0 2/4 1,1 -14 and 318 (mean-152) 2.0 0/4 -- -106 to 212 (mean-64) -- - Gross Pathology - In victims, lungs red; livers mottled red and light red. In survivors, nothing remarkable. Conclusions - Moderately toxic following acute covered percutaneous application. Report 44-50 Page 3 Skla Irritation, Rabbit, Dncovrd Conditions - Standard. Applied in mineral oil. Results - No irritation on 5 rabbits from a 25X suspension (poor) in mineral oil. 031 v Eye Irritation, Rabbit Conditions - Standard. Instilled as a solid or In mineral oil. Results - Trace corneal fluorescein staining (indicating epithelial denudation) in 2 of 5 eyes from 40 mg par eye of the solid particulates; no injury in 5 eyes from 0.5 ml per eye of a 252 suspension (poor) in mineral oil. 1 ucc 062698 Standard Teat Procedures 333 In all tests, tha nonfasted animals ara maintainad on appropriata dlats and watar ad lib except during pariods of manipulation or conflnaaant* Dosage lavala diffar by a factor of 2 in a gaomatric series. LD50s or LC50s, along with tha 95Z confidanea limits, ara calculated by tha moving average method based on a 14-day observation period. Weight changes for survivors were determined at 14 days following dosing. Toxicity Terminology for Peroral and 24-Hr Dermal LD50a (A); Inhalation 4-Hr LC50 (B) Extremely low order VuA, xm/kg B. com "tiQOM Highly P-x.EES 0.05-0.5 106-1M Slightly 5-15 10M-100M Seriously 0.001-0.05 10-100 Moderately 0.5-5 1M-10M Dangerously < 0.001 < 10 Peroral. Compounds administered by stomach intubation to Wistar-derived male rats, 90-120 grams in weight and 3 to 4 weeks of age. Percutaneous. Male New Zealand White rabbits, 3 to 5 months of age, are immobilized during the 24-hour contact period with the compound retained under Impervious sheeting on the clipped, intact skin of the trunk. Thereafter, excess fluid is removed to prevent ingestion. Maximum dosage that can be retained is 16 al/kg. . inhalation. Procedure A. Substantially saturated vapor is generated in a gas washing bottle by passing dried air at 2.5 liters/min through a fritted glass disc immersed to a depth of at least 1.5 Inches in the chemical. This air is delivered to rats in a 9-liter glass exposure chamber. Mean vapor concentration is calculated from the loss in weight of the liquid or estimated from the vapor pressure at the actual temperature of the chemical during aeration. Procedure B. Substantially saturated vapor is prepared by spreading 100 gm or more of chemical over 200 cm^ area on shallow tray placed near the top of a 120-liter plexiglas chamber which is then sealed for at least 16 hours while an Intermittently operated fan agitates the internal chamber atmosphere. Rats are then introduced in a gasketed drawer-type cage designed and operated to minimize vapor loss. Procedure C. Mist, vapor and any oxidation or decomposition products of the chemical held at 170*C are generated and delivered as in A. Procedure D. Vapor at metered concentration, not verified analytically, is generated by feeding the liquid at a constant rate down the inside of a spirally corrugated surface of a minimally heated one-inch Pyrex tube, through which metered air is passed. Resultant vapor is delivered as in A. Procedure E. Aerosols are produced when solutions or suspensions are atomized in a glass VAPONEFRIN nebulizer using dried compressed air at 9 liters/min (corrected) and 22 psi. The resultant aerosol of droplets averaging 2 microns in diameter is conducted directly into a 60-liter cubic glass chamber containing rats. Mean aerosol concentration is calculated from the amount of material atomized. Procedure F. Dust clouds are generated by a baffled Wright Dust Feed through which air is passed at 14 liters/min (uncorrected) at 5 psi. The dust is delivered directly to a 120-liter plexiglas chamber containing rats. Airborne dust concentra tions are measured gravlmetrically every half hour. Skin Irritation. The chemical is applied in 0.01 ml amounts to clipped, uncovered Intact skin of 5 rabbit bellies either undiluted or in progressive dilutions of 10, 1, 0.1, or 0.01Z in solvent. One of 10 grades is assigned based on appearance of moderate or marked capillary injection, erythema, edema or necrosis within 24 hours. No injury from undiluted - Grade 1. Eye Irritation. Eyes not staining with 5Z fluorescein in 20 seconds contact are accepted. Single instillation of 0.005, 0.02, 0.10 or 0.5 ml undiluted or of 0.5 ml of 40, 15, 5 or 1Z dilutions are made into the conjunctival sac of 5 rabbits. The eyes are read immediately unstained and after fluorescein at 24 hours, with ne of ten grades assigned. Trace or no injury from 0.5 ml undiluted " Grade 1.