Document Yrx3ZxYxdRKemBJ8J45yV0L68

BACK TO MAIN BACK TO MAIN Z. GOOD LABORATORI PRACTICE COHPL1ATCK 8TATEMEKT This study was conducted according to EPA Good Laboratory practice Regulations (40 CFR 792), with the following exceptions. The stability of the test substance was assumed but not verified at T.R. wilbury Laboratories and the date of one of the 3 micropipette calibrations can not be verified from the raw data (the year was apparently entered incorrectly). Deviations from the study protocol are discussed in section XII of this report. Jeanne p . Hagaiu ) Study Director L T.R. Wilbury Study Number 841-TH Page 2 of 24 II. QUALITY ASSURANCE STATEMENT submitted by: T.R. wilbury Laboratories, Inc. 40 Doaks Lane Marblehead, Massachusetts Certification: T.R. Wilbury Laboratories, Inc. study number S41-TH, Growth and Reproduction Toxicity Test with L-13492 and the Freshwater Alga, selenastrum capri cornutum, was audited by the Quality Assurance Unit for compliance with the protocol, standard operating procedures, and applicable Good Laboratory practices (U.s. EPA, 1993). The original raw data and a copy of the final report will be archived at T.R. wilbury Laboratories, Inc. for at least 10 years. Quality assurance audits were performed and the findings reported to T.R. Wilbury Laboratories management and the study director on the following dates: Aspect Audited Audit Date Reported to study Director Reported to Management Protocol: In-life: Draft Report: Final Report: 6/ 8/95 6/30/95 7/11/95 8/ 3/95 6/ 9/95 6/30/95 7/11/95 8/ 3/95 6/9/95 8/3/95 8/3/95 8/3/95 A r t h u r P. P a r a d ice Quality Assurance Officer T.R. wilbury study Number 841-TH Page 3 of 24 BACK TO MAIN XXZ. T A U X or CONTkMTS SECTIOW: I . Good Laboratory Practice Compliance Statement II. Quality Assurance statement III. Table of Contents IV. Index of Tables and Figures V. Summary VI. General Information VII. Introduction v i n . Methods and Materials IX. Results X. References XI. Signature page XII. Protocol Deviations Appendix A. Water Quality Data from Toxicity Test PACE 2 3 4 5 6 7 8 8 13 19 20 21 T.R. Wilbury study Number 841-TH Page 4 of 24 IV, IUDII or TABUTS AHO FIGURES Table 1. Characterization of a representative sample of test media and water used to formulate test media for the toxicity tost with the freshwater alga, Selenastrum capricornutum, and L-13492 Table 2. Test system summary for the toxicity test with L-13492 and the freshwater alga, Selenastrum capricornutum Table 3. Cell growth data from the toxicity test with L-13492 and the freshwater alga, Selenastrum capricornutum Table 4. Cell growth data from the toxicity test with isopropyl alcohol and the freshwater alga, Selenastrum capricornutum Table S. Average specific growth rate and percent change from the control from the toxicity test with the freshwater alga, Selenastrum capricornutum, and L-13492 Table 6. Effective concentrations (EClOs, ECSOs, EC90s) from the toxicity test with the freshwater alga, Selenastrum capricornutum, and L-13492 Table A. 1. Temperature measured during the toxicity test with L-13492 and the freshwater alga, Selenastrum caprl cornu turn Table A.2. pH values measured during the toxicity test with L-13492 and the freshwater alga, Selenastrum capricornutum F i g u r e l . Growth of Selenastrum capricornutum exposed to L-13492 for 96 hours T.R. wilbury study Number 841-TH Page V. SUMMARY The toxicity of L-13492 to the freshwater alga, Salmnaatrua caprlcornutua, is described in this final report. The teat was conducted for 3M Company for 96 hours from June 26 to 30, 1995, at T.R. Wilbury Laboratories, Inc. in Marblehead, Massachusetts. It was conducted by Jeanne Magazu, Jacqueline Nevius, Peter Kowalski, Raven Stevens, Peter Nevius, Robert Boeri, and Timothy Ward according to the protocol developed for T.R. wilbury Study Number 841-TH. L-13492 was supplied by the sponsor. The test was performed under static conditions with five concentrations of test substance and a dilution water control at a temperature of 24 t 2*C. Nominal concentrations of L-13492 were 0 (control), 1.0, 2.0, 4.0, 8.0, and 16 mg/L. The dilution water was sterile enriched media adjusted to a pH of 7.5. Cell counts were made da.iy with a hemocytometer. Exposure of algae to the test substance for 96 hours resulted in a median effective concentration (EC50) of 4.9 mg/L L-13492, with 95% confidence limits of 3.5 and 6.7 mg/L. The 96 hour no observed effect concentration (NOEC) is 1.0 mg/L and the lowest observed effect concentration (LOEC) is 2.0 mg/L. A toxicity te3t was performed simultaneously with isopropyl alcohol, a component that represents 27% of L-13492. Algal growth was not affected at 4.4 mg/L (the concentration of isopropyl alcohol present in the 16 mg/L L-13492 solutions) or at 1,000 mg/L, indicating that the concentration of isopropyl alcohol in L-13492 can not, by itself, account for the toxicity of L-13492 to algae. T.R. wilbury Study Number 841-TH Page 6 of 24 BACK TO MAIN VZ . GENERAL INFORNATICI Material Tested: Sponsor: Test Substance Shipped From: Reported Purity: study Initiation Date: Experimental Start Date: Experimental Completion Date: Study Completion Date: Archive Location for the Biological Raw Data and a Copy of the Final Report: L-13492 3M Company Environmental Laboratory 935 Bush Avenue Building 2-3E-09 St. Paul, Minnesota 55144 3M Company Environmental Laboratory 935 Bush Avenue Building 2-3E-09 St. Paul, Minnesota 55144 100% June 14, 1995 (protocol signed) June 26, 1995 June 30, 1995 August 3, 1995 T.R. Wilbury Laboratories, Inc. Marblehead, Massachusetts T.R. Wilbury Study Number 841-TH Page 7 of 24 VII INTRODUCTION This study was sponsored by 3H Company. The objective of the study was to determine the 24, 48, 72, and 96 hour effective concentrations (ECIOs, EC50a, EC90s) and no observed effect concentration (NOEC) of the test substance to the freshwater alga, Selenaatrua capricornutua, under static conditions. The test protocol was based on U.S. EPA methods (EPA, 1993). The report contains sections that describe the methods and materials employed in the study, and results of the investigation. The report also contains an appendix that presents water quality data collected during the test. VIII. METHODS AHD MATERIALS TEST SUBSTANCE: The sample of L-13492 (T.R. wilbury Laboratories Sample Number 502) was delivered to T.R. Wilbury Laboratories on June 14, 1995 in a cardboard box at ambient temperature. It was contained in a 125 ml plastic bottle. The label attached to the bottle included the following information: "L13492, Hazardous Components/Special Precautions: Isopropyl Alcohol, Originator: S.J. Martin - S.A. Beach, 53-4N-02 -* 2-3E-09, 8-5774, 8-7065, 5/4/95, 6/7/95, N2332-1-. L-13492 (a thin, clear liquid) was supplied by 3M Company, St Paul, Minnesota. Prior to use the test substance was stored in the dark at room temperature. Test substance stock solutions were formulated in dilution water without the use of a solvent. The test substance was assumed to have a purity of 100 active ingredient and to be stable under storage and testing conditions. Unused test substance will be returned to the sponsor. DILUTION WATER: Water used for acclimation of test organisms and for all toxicity testing was sterile enriched media (U.s. EPA, 1978; T.R. Wilbury standard Operating Procedure number 6) adjusted to a target pH of 7.5 with 0.1 N HCl prior to use. Characterization of a representative sample of media is presented in Table 1. TEST ORGANISM: Algae used for the test (Selenastrum caprlcornutum, UTEX 1648) was from a culture originally procured from the Culture Collection of Algae at the University of Texas at Austin and delivered to T.R. Wilbury Laboratories on April 11, 1995. The culture was transferred to sterile enriched media identical to media used for this test and maintained at test conditions for at least 14 days before the definitive test. The subsample of algae used to inoculate media at the start of the definitive test came from a 7 day old culture. Identification of the culture organisms was verified using an appropriate taxonomic key. T.R. wilbury study Number 841-TH Page 8 of 24 BACK TO MAIN Table 1. Chemical characterization of a representative sample of test media and water used to formulate test media for the toxicity test with the freshwater alga, ielenastrum capricornuttm, and L-13492. Parameter Unit of Measurement Detection Limit Measured Value pH Total Phosphorus Nitrate Chloride Tocal Organic Carbon Metals Arsenic Cadmium Copper Fluoride Soron Aluminum Chromium Cobalt Iron Lead Mercury Nickel silver Zinc Organochlorine Pesticides plus Toxaphene Organophosphorus Pesticides PCB3 pH units mg/L mg/L as N mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L pg/L g/L pg/L pg/L 0.03 0.05 i 1 0.01 0.0002 0.005 0.1 0.5 0.1 0.01 0.03 0.03 0.005 0.0003 0.03 0.02 0.02 0.5 2 0.52 0.5 7.5 0.46 0.08 14 NO ND 0.0002 ND ND ND ND ND ND 0.08 ND ND ND ND ND ND ND ND ND Notes: 1. The pH was measured in a control test vessel at the start of the test. Total organic carbon. total phosphorus, nitrate. chloride. fluoride, metals, pesticide, and PCB data vere collected during February, 1995, as part of routine biannual water quality testing (these analysis were performed by Pace, Inc., Hampton, New Hampshire). 2. ND = not detected at or above the detection limit. T.R- Wilbury Study Number 841-TH Page 9 of 24 BACK TO MAIN Results of a range finding test were used to select the concentrations for the definitive tent. The range finding test was performed from June 15 to 19, 1995* At the conclusion of the test the number of cella/ml equalled the following percent of the control? 0.10, and 1.0 mg/L * >89%, 10 mg/L - 4%, 100 mg/L - 2%, and 1,000 mg/L * <1%. The definitive toxicity test was performed from June 26 to 30, 1995, according to T.R. Wilbury Protocol 841-TH (Growth and Reproduction Toxicity Test with L-13492 and the Preshwater Alga, selenastrum capricornu turn). The protocol, which was signed by the study director on June 14, 1995, is based on procedures of the U.S. Environmental Protection Agency (1993). A summary of the test system is presented in Table 2. The test was conducted at a target, temperature of 24 t 2*C with five Concentrations of test substance and a dilution water control. Nominal concentrations of the test substance were: 0 mg/L (control), 1.0, 2.0, 4.0, 8.0, and 16 mg/L. A 16 mg/L stock solution was formulated on June 26, 1995 by adding 0.016 g L-13492 to sterile enriched media and adjusting the final media volume to 1,000 ml. Appropriate amounts of this stock solution were added directly to dilution water to formulate test media. A test was performed simultaneously with isopropyl alcohol (Mallinckrodt, Lot 3035), a component that represents 27% of L-13492. This test was conducted at 4.4 mg/L (the concentration of isopropyl alcohol in vessels containing 16 mg/L L-13492) and at 1,000 mg/L. A 1,000 mg/L stock solution was formulated on June 26, 1995 by adding 0.5 g isopropyl alcohol to sterile enriched media and adjusting the final media volume to 500 ml. Algae were distributed among three replicates cf each treatment at the rate of 10,000 cells/ml. The test was performed in 250 ml glass Erlenmeyer flasks that contained 100 ml of test solution. Test vessels were randomly arranged on a rotary shaker adjusted to 100 rpm in an incubator during the test (a random numbers table was used to select the location of each vessel). A 24 hour light and 0 hour dark photoperiod was automatically maintained with cool-white fluorescent lights that provided a light intensity of approximately 350-360 footcandles. The number of algal cells/ml in each te3t vessel and the occurrence of relative size differences, unusual cell shapes, colors, flocculations, adherence of cells to test containers, or aggregation of cells was determined visually by means of direct microscopic examination with a hemocytometer. Cell counts were made and recorded daily during the 96 hour test. The determination of whether toxic effects were algistatic or algicidal was performed at the conclusion of the toxicity test (0.5 ml of media from each 16 mg/L vessel was transferred to a vessel containing fresh media without L-13492 and incubated for 192 hours under test conditions). T.R. Wilbury study Number 841-TH Table 2. Teat B y t e a summary for the toxicity teat with 1-13492 and the freahwater alga, Solenastnm capricornuturn. Species: Selenaatrum capricomutum Culture Acclimation Period: >14 days Test Duration: 96 hours Test Vessels: 250 ml glass flasks Volume of Test Media: 100 ml Number of Replicate Test Vessels: 3 Inoculum: 10,000 cells per ml Oscillation Rate: 100 rpm Incubator Temperature Range: 23.4-23.7*C Photoperiod: 24 hours light and 0 hours dark. Light Intensity: 400 footcandles Water Quality Measurements: pH in each test vessel at the beginning and end of the test. Incubator temperature daily. Dilution water analysis is summarized in Table 1. End Points: 24, 48, 72, and 96 hour EC10, EC50, and EC90; no observed effect concentration. Acceptability Criteria: Logarithmic growth at 96 hours and acceptable temperature range. T.R. Wilbury Study Number 841-TH Page 11 of 24 Temperature of the incubator was Pleasured and recorded daily (thermometer number 2968), and pH (Beckman model pBI 12 meter; instrument number 124) was determined in each test vessel at the beginning and end of the test. The temperature in a representative vessel of water incubated with the teat vessels was continuously recorded. STATISTICAL METHODS: The average specific growth rate was calculated as the natural log of the number of cells/ml at 24, 48, 72, and 96 hours minus the natural log of the number of cells/ml at 0 hours divided by the exposure period. The percent change from the control was calculated by subtracting the treatment average specific growth rate from the control average specific growth rate, dividing the difference by the average specific growth rate in the control, and multiplying that value by 100. Results of the toxicity test were interpreted by standard statistical techniques. The statistical method used for calculating 24, 48, 72, and 96 hour EC values (and confidence intervals) was based on a nonlinear regression estimation procedure (Bruce and Versteeg, 1992). All calculations were performed using the average specific growth rates and nominal concentrations of L-13492. The no observed effect concentration (NOEC) was determined using a parametric one-way analysis of variance (ANOVA) and average specific growth rate in each test vessel at the end of the test. T.R. Wilbury Study Number 841-TH Page 12 of 24 IX. RESULTS No insoluble material was noted in any test vessel during the test. The algal population in the control test vessels grew well, resulting in an average of 1,249,000 cells/ml after 96 hours (Table 3). Water quality throughout the test was within acceptable limits (Appendix A ) . The range of incubator temperatures was 23.4 to 23.7*C (Table A.l) and the pB of test media was not affected by the test substance at the beginning of the test (Table A.2). Biological data generated by the acute toxicity test with L-13492 are presented in Tables 3 and 4. The growth curve for algae exposed to the test Bubstance for 96 hours is presented in Figure 1 (the slope of the dose-response curve is 3.3). The 48, 72, and 96 hour EC10, EC50, and EC90 values for algae exposed to L-13492 are presented in Table 6. The 96 hour EC50 (and associated 95 percent confidence limits) is 4.9 mg/L (3.5 and 6.7 mg/L). No effects (size differences, unusual cell shapes, colors, flocculations, adherence of cells to test containers, or aggregation of cells) were noted. The 96 hour no observed effect concentration (NOEC) is 1.0 mg/L and the lowest observed effect concentration (LOEC) is 2.0 mg/L L-13492. The determination of whether toxic effects were algistatic or algicidal was performed at the conclusion of the toxicity test. A 0.5 ml aliquot of media from each 16 mg/L vessel was transferred to a vessel containing fresh media without L-13492 and incubated for 192 hours under test conditions. Algae increased from 400 cells/mL to 580,000 cells/mL, indicating that the effect of L-13492 at this concentration was algistatic rather than algicidal. Biological data generated by the exposure of algae to isopropyl alcohol are presented in Table 4. Algal growth was not affected at 4.4 mg/L or 1,000 mg/L, indicating that the concentration of isopropyl alcohol in L-13492 can not, by itself, account for the toxicity of L-13492 to algae. T.R. wilbury study Number 841-TH Page 13 of 24 BACK TO MAIN Tabla 3. call growth data from tha acute toxicity teat with L-13492 and the fraahvatar alga, Salenaatrua caprlcornutum. Nominal Concantration Of L-13492 (mg/L) rep. Number of Cella/ml :[ 101 (hour) 0 24 48 72 96 0 (control) 1.0 2.0 4.0 8.0 16 1 2 3 mean t control 1 2 3 mean % control 1 2 3 mean control 1 2 3 mean % control 1 2 3 mean control 1 2 3 mean % control 10 10 10 10 -- 10 10 10 10 100 10 10 10 10 100 10 10 10 10 100 10 10 10 10 100 10 10 10 10 100 22 20 23 22 -- 20 18 28 22 100 11 18 19 16 73 26 19 14 20 91 18 16 21 18 82 12 11 18 14 64 134 538 1,132 132 568 1,260 114 614 1,356 127 573 1,249 ------ 142 606 1,378 112 592 1,156 118 610 1,400 124 603 1,311 98 105 105 140 178 270 102 198 252 136 202 304 126 193 275 99 34 22 86 100 106 78 160 102 80 104 132 81 121 113 64 21 9 44 66 98 52 72 80 40 48 74 45 62 84 35 11 7 34 40 24 38 70 34 34 38 22 35 49 27 28 9 2 T.R. wilbury Study Number 841-TH Page 14 of 24 j : Table 4. Call growth data from tha acuta toxicity taat with iaopropyl alcohol and tha fraahwatar alga, Salanaatrua caprlcornutua. Nominal Concantration of 1,-13492 (mg/L) rep. Number of Cells/ml x 101 (hour) 0 24 48 72 96 0 (control) 4.4 1,000 1 2 3 mean control 1 2 3 mean * control 1 2 3 mean control 10 22 10 20 10 23 10 22 ---- 10 14 10 32 10 16 10 21 100 95 10 31 10 26 10 24 10 27 100 123 134 538 1,132 132 568 1,260 114 614 1,356 127 573 1,249 ---- -- 112 584 1,236 128 554 1,270 144 584 1,064 128 574 1,190 101 100 95 136 608 1,370 144 580 1,338 106 512 1,334 129 567 1,347 102 99 108 T.R. wilbury study Number 841-TH Page 15 of 24 4 BACK TO MAIN BACK TO MAIN Tabla S. Average specific growth rats and percent change fron the control from the toxicity test with the freshwater alga, Salenamtrua capricornutum, and L-13492. Nominal Average Specific Percent Change Concentration Growth Rate From control of L-13492 1 (mg/L) 24hr 48hr 72hr 96hr 24hr 48hr 72hr 96hr I 0 (control) 0.032 0.053 0.056 0.050 ------ -- 1.0 0.032 0.052 0.057 0.051 0 2 -2 0 2.0 0.018 0.053 0.041 0.035 44 0 27 30 4.0 0.027 0.04 4 0.035 0.025 16 17 37 50 8.0 0.025 0.031 0.025 0.022 22 42 55 56 16 0.012 0.026 0.022 0.010 63 51 61 80 T.R. wilbury study Number 841-TH Page 17 of 24 BACK TO MAIN Table 6. Effective concentrations (EClOs, ECSOs, EC90s| from the toxicity test with the freshwater alga, Selenaatrua c.ipricornu turn, and L-13492. Time EC Value (mg/L) 95 Percent Confidence Limits (mg/L) CO 24 hours hours 72 hours 96 hours EC 10 as <1.0 EC50 - 15 EC90 a >16 EC 10 <1.0 ECSO - 14 EC90 >16 EC10 a <1.0 EC50 as 7.1 EC90 " >16 EC 10 as <1.0 EC50 m 4.9 EC90 >16 <1.0 9.8 - >16 7.7 - >16 <1.0 8.2 - >16 >16 <1.0 4.7 - 11 >16 <1.0 3.5 - 6.7 >16 T.R. Wilbury Study Number 841-TH Page IB of 24 BACK TO MAIN BACK TO MAIN XI. SIGNATURE PAGE /.!'//< u . t'ltf Jeanne P. Magazu * Study Director Aquatic Toxicologist ' ( d j / f\Robert L. Boeri Coauthor Z5-? ^ ft Peter . Kowalski i *>5 Aquatic Toxicologist I ., t \ . '. 1 ; f , . ; `i . * , -K, i' V _ ' for: Jacqueline' M . 1Nevius Aquatic Toxicologist i./ / V/ 1` Keven M. Stevens Biologist r - *>Trri - S'/ 5 / W a-- Peter C. Neviu3 Biologist /< ;/ '' / / >// J ^ Tisflothy J. Ward Author sA/W T.R. Wilbury Study Number 841-TH Page 20 of 24 BACK TO MAIN E. XII. PROTOCOL AMENDMENTS AMD DXV1AZIOMS PROTOCOL AMENDMENTS! The protocol was amended to specify the nominal concentrations for the definitive test and to note that a test would also be conducted with 4.4 and 1,000 mg/L isopropyl alcohol. PROTOCOL DEVIATIONS! No deviations were made from the protocol. T.R. Wilbury Study Number 841-TH Page 21 of 24 BACK TO MAIN Appendix Al HATER QULITX DATA EROM TOXICITY TEST I L t , i \ T.R. Wilbury Study Number 841-TH Page 22 of 24 BACK TO MAIN Table A.l. Temperature measured during the toxicity test with L-13492 and the freshwater alga, Selenaatrwa c*prlcornuttm. Hour of Exposure Temperature of Incubator (*C) 0 23.6 24 23.6 48 23.4 72 23.4 96 23.7 T.R. Wilbury study Number 841-TH Page 23 of 24 BACK TO MAIN Tabla A.2. pH value* measured during the toxicity test with L-13492 and the freshwater alga, Saimaa true caprlcornutaa. Nominal Concentration (mg/L) Replicate pH Initial Final Test with L-13492 0 (control) 1.0 2.0 4.0 8.0 16 i 2 3 1 2 3 1 2 3 1 2 3 1 2 3 1 2 3 7.5 7.5 7.5 7.5 7.5 7.5 7.5 7.5 7.5 7.4 7.4 7.4 7.4 7.4 7.4 7.4 7.4 7.4 9.8 10.4 10.0 9.8 9.9 9.8 8.6 8.3 8.2 8.0 8.0 7.9 7.7 7.7 7.7 7.6 7.6 7.6 o o*4 Test with Isopropyl Alcohol 4.4 1 2 3 1,000 i 2 3 7.4 7.4 1C.1 7.4 10.5 7.4 10.4 7.4 9.9 7.4 9.9 T.R. wilbury Study Number 841-TH Page 24 of 24 U4/U1 /UU1---------------bNVlHUNMbNIAL LABUHAIUHV ARCHIVED FINAL REPORT T BACK TO MAIN LAB REQUEST NO. N2332 REQUESTOR NAME: SAB/S.J.MARTIN DEPARTMENT: 3048 PROJECT NO: BI703004UQ DATE RECEIVED: 06/07/1995 DESC: L-13492 ECOTOXICITY EVALUATION CONTRACT LAB(S): TRWIL EXP COMP DATE: 08/30/1995 DATE COMPLETED: 09/02/1995 PROJECT LEAD: PHONE NO: 3M FAX NO: 651-778-6176 Tests conducted following 40CFR 797; methods 1050, 1300, 1400. Sample desc.: L-13492, 45% solids, from S. Martin. SAB 6/7/95 Sample is a clear liquid. Lot confirmed as Lot #2 as per S.J. Martin, of 3M SCD Lab on 7/18/95. SAB Results from 48-hr Daphnia magna acute toxicity test: Test Cone., mg/L 0 (control) 13 22 36 60 100 Percent Immobilization and/or Mortality 24-hrs Exposure 48-hrs Exposure 00 00 55 0 55 0 100 -HT 7o 100 sab 7/31/95 Results from 96-hr Fathead minnow acute static toxicity test: Test Cone. mg/L 0 (control) 130 220 360 600 1000 24-hrs exp 0 0 0 0 0 50 Percent Mortality 48-hrs exp 72 -hrs exp 00 00 00 00 00 60 70 96-hrs exp 0 0 0 0 0 70 sab 7/31/95 Results from 96-hr algae growth inhibition test: Test cone. mg/L 1.0 2.0 4.0 8.0 16 EC50 95% C.I. sab 9/1/95 Percent change from Control 24-hrs exp. 48-hrs exp. 72-hrs exp. 96-hrs exp. 02 -2 0 44 17 27 30 16 17 38 50 22 42 55 56 63 51 61 80 15 14 7.1 4.9 (9.8 - >16) (8.2 - >16) (4.7 - 11) ' (3.5 - 6.7) SAMPLE DATE CODE DESCRIPTION RESULT * = CONTRACT LAB DATA 04/01/2001 LAB REQUEST NO. N2332 SAMPLE DATE CODE ENVIRONMENTAL LABORATORY ARCHIVED FINAL REPORT CONTINUED DESCRIPTION RESULT 1 05/04/95 L-13492 Lot 2 TRWIL 48-HR-EC50 *DAPHNIA ACUTE TEST TRWIL 96-HR-LC50 *FISH ACUTE TEST - 96 HOUR TRWIL IC50-ALGAE *ALGAE ASSAY - IC50 34 MG/L 890 MG/L 4.9 MG/L * = CONTRACT LAB DATA BACK TO MAIN MDL or (95% C.I.) 2 MDL or (95% C.I.) (30 - 39) (600 - >1000) (3.5 - 6.7)