Document YrnzObLoZYYqOY7xx6Vvvwdq0
Naval Research Laboratory
Washington, DC 20375-5320
N R L /M R /6 1 8 0 --00-8474
Evaluation of the Effects of AFFF Inputs on the VIP Biological Nutrient Removal Process and Pass-Through Toxicity
M . E rten-U nal G .C . SCHAFRAN
Old Dominion University Norfolk, VA
F .W . W illiams
Navy Technology Centerfor Safety and Survivability Chemistry Division
August 11, 2000
Approved for public release; distribution is unlimited.
__________ I US00000956
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1. A G E N C Y U S E O N L Y (Leave Blank) 2 . R E P O R T D A T E
3. R E P O R TT Y P E AN D DATES C O V E R E D
August 11,2000
Final Report
4. TITLE A N D S U B T IT L E
Evaluation of the Effects of AFFF Inputs on the VIP Biological Nutrient Removal Process and Pass-Through Toxicity
5. F U N D IN G N U M B E R S
6. A U T H O R (S)
M. Erten-Unal,* G.C. Schafran,* and F.W. Williams
7. P E R F O R M IN G O R G A N IZ A T IO N N A M E (S ) A N D A D D R E S S (E S )
Naval Research Laboratory Washington, DC 20375-5320
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NRL/MR/6180-00-8474
9. S P O N S O R IN G /M O N IT O R IN G A G E N C Y N A M E (S ) A N D A D D R E S S (E S )
Naval Facilities Engineering Command Washington Navy Yard Washington, DC 20374-5065
11. SU PP LEM EN TA R Y N O TE S
*01d Dominion University, Norfolk, VA
12a. D IS T R IB U T IO N /A V A IL A B IL IT Y S T A T E M E N T
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12b. D IS T R IB U T IO N C O D E
1 3 . A B S T R A C T (Maximum 200 worts)
This report discusses the results of a bench scale study conducted to evaluate the potential inhibitory effects of pretreated Aque ous Film Forming Foam (AFFF) wastewater to the Virginia Initiative Plant (VIP) biological nutrient removal process. The pretreat ment scenarios included use of defoamers and use of Fenton's reagent as a strong oxidant. Under this testing, bench-scale reactors simulating the nitrification process were loaded with three AFFF concentrations that were pretreated by defoamers and three AFFF concentrations treated with Fenton's reagent. The pretreatment results with defoamers demonstrated that the effluent ammonia nitro gen concentrations for Defoamer #8710 were higher than the effluent ammonia levels for the Defoamer AF 9020, indicating better pretreatment and less nitrification inhibition for the latter defoamer. Fenton's reagent was used to pretreat AFFF at concentrations of 60 ppm, 120 ppm and 480 ppm. There was no nitrification inhibition at 60 ppm and 120 ppm for AFFF wastewater pretreated with Fenton's reagent as compared to the controls. Oxidation with Fenton's reagent was more effective than the defoamers used in pretreating AFFF. The nitrification inhibition potential decreased at concentrations greater than 60 ppm; however, nitrification inhi bition occurred at 480 ppm AFFF pretreated with Fenton's reagent. In all of the pretreatment alternatives used, the inhibition reactor effluents exhibited pass-through toxicity to mysid shrimp at AFFF concentrations of 60 ppm or greater, whereas the effluent was not toxic to the sheepshead minnows.
14. SU B JEC T TE R M S
Environmentally friendly Aqueous film forming foam Toxicity
17. S E C U R IT Y C L A S S IF IC A T IO N OF REPORT
18. S E C U R IT Y C L A S S IF IC A T IO N O F T H IS PAGE
UNCLASSIFIED
UNCLASSIFIED
NSN 7540-01-280-5500
19. S E C U R IT Y C L A S S IF IC A T IO N OF ABSTRACT
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15. NUM BER O F PAGES
672
16. P R IC E C O D E
2 0 . L IM IT A T IO N O F A B S T R A C T
UL
Standard Form 298 (Rev. 2-89) Prescribed by ANSI Std 239-18 298-102
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TABLE OF CONTENTS
1.0 INTRODUCTION...................................................................................................... 1.1 O v erv iew ........... . .................................................................. . .......................... 1.2 Study O bjectives.................... .............................................................................
2.0 METHODS AND M A T ER IA L S.............................................................................. 2.1 Reference Reactor Operation ........................................................................... 2.2 Analytical M eth o d s.............................................................. .............................. Butyl Carbitol Analysis............................................................................. 2.3 BNR Inhibition Batch Assays ......................................................................... 2.4 Toxicity Pass-Through T e stin g ........................................ ................................
3.0 R E S U L T S ...................................................................................................................
3.1 Reference Reactor Performance .....................................................................
3.2 Butyl Carbitol A nalysis.....................................................................................
3.3 BNR Inhibition Batch Assays Pretreated with Defoamers ...........................
3.3.1
Pretreatment with Defoamer 8 7 1 0 ...............................
3.3.2
Pretreatment with Defoamer AF9020 .........................
3.3.3
Pretreatment with Best Performing D e fo am er...........
3.4 BNR Inhibition Batch Assays Pretreated with Fenton's R e a g e n t................
3.4.1
Pretreatment with Fenton's Reagent at 60 ppm AFFF
3.4.2
Pretreatment with Fenton's Reagent at 120 ppm AFFF
3.4.3
Pretreatment with Fenton's Reagent at 480 ppm AFFF
3.5 Toxicity Pass-Through T e stin g ........................................................................
4.0 SUMMARY and CONCLUSIONS ............................. ...................................
REFEREN CES..................................................................................................................
Appendix A AFFF Butyl Carbitol Analyses
Appendix B BNR Inhibition Batch Assays Pretreated with Defoamers
Appendix C BNR Inhibition Batch Assays Pretreated with Fenton's Reagent
Appendix D Pass-Through Acute Toxicity Tests
.1 .1 .3
.4 .4 .6 .7 10 11
12 12 14 14 15 18 21 24 24 27 27 32
34
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US00000958
1.1 O verview
1.0 INTRODUCTION
A surfactant, commonly referred to as AFFF (aqueous film-forming foam)[l], is widely
used by the U.S. Navy in fire fighting water to improve the ability to control petroleum-based
fires. The US Navy is exploring a number o f options that include disposal o f the fire fighting
water to wastewater collections systems where the components o f AFFF wastewater would be
removed biologically. Disposal o f the fire fighting foam to sanitary sewers has been considered
as an option, however, concern for the potential toxic or inhibitory effects associated with AFFF
wastewater have generally led to a ban from introduction o f AFFF to wastewater collection
systems. Present concerns over inhibitory effects o f AFFF wastewater have resulted in the
prohibition o f its disposal to the Hampton Roads Sanitation District (HRSD) collection system
where it would eventually enter one o f the treatment plants that has nitrifying organisms.
Several studies have been performed on the disposal and treatment o f AFFF surrogate
wastewater. Bench-scale anaerobic and aerobic reactors were used to investigate the potential
inhibition o f the untreated and pretreated AFFF surrogates (AFFF-S) to nitrification,
denitrification, and phosphorus release and uptake in a biological nutrient removal (BNR) process
(2,3). The results showed that untreated AFFF-S wastewater at concentrations similar to that
expected from the firefighting events exhibited measurable inhibitory effects on biological
nitrification. The use o f coagulants such as alum, ferric chloride, calcium chloride and cationic
polymers have also been observed to be capable o f reducing the organic content o f AFFF. Upon
chemical pretreatment and using aerobic and anaerobic sequencing batch reactions, on acceptable
effluent ameuable to an aquatic stream was processed [6, 7 and 8], Treatability studies have also
been conducted with a high-purity oxygen activated sludge system [9]. The results showed 73 to
96% biological oxygen demand (BOD) removal and 69 to 76% chemical oxygen demand (COD)
removal. The use of dissolved air flotation treatment on the firefighting wastewater further
reduced the dilution ratio needed for acceptable effluent quality from the biological process [10],
Additional studies that were performed by the Air Force to determine the biodegradability
of AFFF wastewater (11, 12, 13, 14 and 15) demonstrated that nitrification was evident and no
Manuscript approved July 14, 2000.
1
US00000959
toxicity to fathead minnows was obtained at 250 ppm AFFF concentration in the wastewater. Application o f physical-chemical treatability studies that were performed with two
different types o f AFFF (FC-206 and AOW-6 waste) included chemical coagulation and flocculation (precipitation), clarification, carbon adsorption, chemical oxidation and air stripping ([16]. In these studies, chemical oxidation by chlorine removed only 8% COD and oxidation by potassium permanganate removed 14% o f the COD. Air stripping, using both air and nitrogen sparging demonstrated only 10% COD reduction after 24-hours o f aeration for both techniques.
Fenton s reagent is used for generating strong oxidants in aqueous solutions has been well documented. Studies using solutions o f pure compounds and industrial wastewaters indicated very efficient destruction o f the original compounds during oxidation [17], Fenton's reagent Has been applied to treatment o f several wastewaters containing organic contaminants including oxidation o f di- and tri- chlorophenols in aqueous solution [18].
Recurrent study was performed in two phases which will be referred to as Phase LA and Phase IB. The primary intent o f Phase LA was to determine the potential inhibitory effect o f untreated AFFF solution on biological treatment and whether toxicity passes through to the effluent in biological treatment processes. This report contains the results o f Phase IB in which the primary intent was to determine the potential inhibitory effect of pretreated AFFF solution on the biological nutrient removal process trader six different operational conditions. The two types o f pretreatment included chemical oxidation with Fenton's reagent and adding different types o f defoamers to AFFF wastewater. It also was investigated whether toxicity passes through to the effluent in the biological treatment process. Phase IA results have already been documented previously [19].
2
1.2 Objectives
The overall objective o f this study was to study the impact of pretreated AFJFF wastewater to a biological nutrient removal process and determine whether pass-through toxicity occurs in the effluent o f a biological process receiving wastewater containing AFFF. Specific objectives o f this study include:
Determine the nitrification inhibition potential of AFFF wastewater that is pretreated with two different types o f defoamers and the degree o f COD removal under operating conditions similar to those o f the VIP plant;
Determine the nitrification inhibition potential o f AFFF wastewater that is pretreated with Fenton's reagent and the degree o f COD removal under operating conditions similar to those o f the VIP plant;
Measure the acute toxicity o f the pretreated AFFF wastewater effluent to Mysidopsis bahia (mysid shrimp) and Cyprinodon variegatus (sheepshead minnow) to assess the possibility o f toxicity pass through in a process similar to the VIP process.
Examine the chemical composition of the AFFF surfactant and determine the butyl carbitol concentration in the reactors dosed with AFFF by ion chromatography and pulsed amperometric detection methods.
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2.0 METHODS AND M ATERIALS 2.1 Reference Reactor Operation
A 100-liter capacity fill-and-draw type batch reactor was used in order to maintain a continuous supply of uniform nitrifying microorganisms at the Environmental Engineering laboratory o f Old Dominion University. The reactor operation was under cyclical anaerobic and aerobic conditions to establish and maintain a nitrifying, phosphorus-accumulating biomass that would show similar nutrient removal performance as in the VIP plant. The reference reactor consisted o f a 30gallon polyethylene tank containing a hexagonal-shaped poly vinyl chloride (PVC) air diffuser and a rapid mixer. The reactor was seeded with a target mixed liquor suspended solids concentration using sludge from the VEP plant to obtain simultaneous nitrogen and phosphorus removal. The solids were allowed to settle and the supernatant was decanted. The reactor was then fed over the duration o f the study with a synthetic feed solution comprised o f organic and inorganic compounds necessary to support a healthy population o f nitrifying, denitrifying and phosphorus removing bacteria. This feed had the same composition used in the first Phase. Table 2-1 shows the organic and inorganic constituents used for preparing the feed solution. The feed was delivered to the reactor over the periods specified each day from a feed tank with a peristaltic pump. The feed tank consisted o f a 30 gallon polyethylene tank which was placed in a refrigerator at 4C to limit bacterial growth.
An appropriate period of time was allowed to stabilize the reference reactor bacterial population. Reactor operation was sequenced with a programmable timer to activate mechanical mixing, aeration, solution feed, and mixed liquor and supernatant withdrawal. In Phase IA, the reactor was operated on a 4-hour cycle of aerated feed anoxic react, aerated react, settle and decant. In Phase IB, the aeration during the feed stage was terminated because non-aerated feed was a more
4
Table 2-1. Synthetic Feed Stock C onstituents
C o n stitu en ts B eef Extract Bactopeptone Urea k h 2p o 4 (n h 4)2c o 3 Na2C03 FeS04 CuS04 Z n S 0 4.7H20
grams/gallon C onstituents
0.5392
M gS04
0.7842
CaCl2.2H20
0.1470
NaCl
0.2818
k 2h p o 4
0.5563
NaHC03
2.2864
CH3COOH
0.0246
M nS04.H20
0.00001
N a 2 M o 0 4.2H20
0.0002
grams/gallon 0.2242 0.0590 0.9836 0.1103 0.8161 0.5688 0.0002 0.00001
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representative operation mode o f the VIP BNR process. React aeration, mixing and decant were all controlled by a programmable controller. Aeration was provided through in-house air supply source and a dissolved oxygen concentration o f approximately 4 mg/L was targeted during the react cycle in the reactor in Phase n. Additional mixing was supplied by a mechanical mixer. Operation o f each cycle comprised o f 4-hour feed with aeration, 4-hour anaerobic, 4-hour aerobic, 4-hour settle and a two-minute decant period. During each cycle, 7.5 gallons o f feed was supplied and the sama amount was decanted as supernatant. The total volume in the reactor was 24 gallons. The feed and supernatant were collected and analyzed for COD and ammonia nitrogen (NH3-N) twice per week.
The reactor was also monitored for mixed liquor suspended solids (MLSS) and sludge volume index (SVI) twice per week. The COD analyses was favored over BOD as it gave very fast and repeatable results. However the BODrCOD ratio was periodically checked for both the feed and the supernatant in order to evaluate the stability o f the ratio.
2.2 Analytical Methods The analytical methods employed in this study for evaluating the effects o f pretreated AFFF
wastewater inputs on biological treatment performance consisted o f procedures as prescribed by the United States Environmental Protection Agency [20] or in Standard Methods [21]. All chemicals used were reagent grade or better and all quality assurance/quality control procedures were followed as closely as possible.
M easurements o f organic strength were determined through carbonaceous five day BOD (CBODs) and COD measurements. CBOD5 (determined with a nitrification inhibitor added to BOD bottles) were measured to eliminate potential interferences that nitrification could have on the
6
evaluation of organics removal with the BOD test. CBOD and COD analyses were determined using filtered samples on reactor effluent and filtered and unfiltered samples in the influent. Samples were filtered through a glass fiber filter to eliminate microorganisms and other particulate materials that are not related to the organic components o f the AFFF or the dissolved organic compounds that are in the wastewater before AFFF introduction. Since the AFFF components are water soluble and will be dissolved in solution, filtration should not directly interfere with their accurate detection. Measurements o f total suspended and volatile suspended solids (TSS and VSS, respectively) were used to determine organic solids loading, reactor MLSS and mixed liquor volatile suspended solids (MLVSS) concentrations and non-settleable TSS concentrations in reactor effluent. In order to reduce variability o f TSS and VSS data, the tests were performed on the same days that solids concentrations were fed into the reactor. The nitrogen series were determined by three different analytical techniques. Persulfate digestion followed by ammonia analysis by ion selective electrode was utilized to determine total Kjeldahl nitrogen (TKN) concentrations, ammonia concentrations were measured by ion selective electrode without sample digestion, and nitrate and nitrite concentrations were determined on filtered samples using ion chromatography. Orthophosphate was similarly determined using ion chromatography.
B utyl Carbitol Analysis As part o f this study, butyl carbitol, a major component o f AFFF, was also analyzed by ion
chromatography utilizing electrochemical detection. Although AFFF from 3M Corporation was used in this study, there are three other Navy-approved manufacturers of AFFF and they have different amounts o f butyl carbitol in their product. The AFFF from 3M Corporation was the most readily
7
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available product during this study. The Material Safety Data Sheet for AFFF, obtained from its manufacturer 3M Corporation, indicates that butyl carbitol (CAS # 112-34-5) is present as diethylene glycol monobutyl ether at 30 percent by volume. The method used was the one developed by the Dionex Corporation which measured alcohols by Pulsed Amperometric Detection (PAD).
In this method, a potential is applied to an electrode (a gold electrode in this case) in which the amperometric cell current is integrated and electrons are actually transferred between the analyte molecules and the electrode. The detector output is then reported for the integration period. Repetitive series of potentials or a user programmed waveform provided by Dionex is applied to the cell. A waveform includes a senes o f oxidation reduction potentials which sets the beginning and end of the integration period as shown in Figure 2-1. In amperometric detection method, the potential difference between the electrode and the solution is high enough to cause electron transfer reactions to occur which oxidizes or reduces the species in solution. During an oxidation reaction, electrons are transferred from the analyte to the electrode whereas during reduction, the reverse occurs. During a sample run, two eluants, 90/10 Acetonitrile/water and 100% water were used. A 0.3 M N aOH solution was used as a post column reagent and OmniPac PAX-500 columns were used as analyte columns. The eluant solutions were applied by using linear gradient method. A graphical representation o f the linear gradient method is shown in Figure 2-2. A copy o f the method used in butyl carbitol determination is in Appendix A.
The procedure used in method development consisted o f initially determining the timft at which the peak for a known concentration of pure butyl carbitol would elute and then determining the concentration of any peak that would elute at the same retention time for the samples containing AFFF. To rule out any interferences that may be caused from any constituent that would elute at the
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same retention time, the synthetic feed solution sample, and the samples from the control reactors which contained no AFFF were also tested with the same method. Results o f the test method are also in Appendix A. The results attached were obtained from inhibition test samples with 60 ppm AFFF and Defoamer AF9020. Pure butyl carbitol was tested at concentrations o f 1 ppm, 10 ppm and 50 ppm. This range was used since the AFFF concentration tested was only 60 ppm and the butyl carbitol concentration in AFFF was only 30% by volume.
2.3 BNR Inhibition Batch Assays In Phase II, six nitrification inhibition tests were performed. O f the six, three inhibition tests
investigated the effect o f defoamers on the treatability o f AFFF, and the other three investigated the use o f Fenton's reagent on removal o f AFFF organic strength and nitrification inhibition. The two defoamers tested were Defoamer DF8710, which is currently used by the Navy, and Defoamer AF9020 which was recommended by the manufacturer o f AFFF used in this study. The inhibition tests started with 60 ppm AFFF concentration since this level was identified as the threshold concentration for nitrification inhibition in Phase IA. Specific doses o f each defoamer were added in proportion to eliminate any foaming caused by AFFF during the aerated operational stage o f the inhibition test.
Fenton's reagent, a strong oxidizing agent, was also investigated as another pretreatment option to determine its effects on COD removal by mixing AFFF laden wastewater with appropriate dose ofFe and H202 (Fenton's reagent) for 24-hours period. Fenton's reagent is formed as a result o f the reaction o f hydrogen peroxide and iron (II) to generate hydroxyl radical [22], Residual H20 2 was neutralized with sodium bisulfite. The pH o f this pretreated wastewater was then adjusted to 7.0
10
by adding sodium hydroxide and this was then blended with synthetic feed solution before it was fed to the reactors. As in Phase IA, three control reactors with no AFFF, no defoamers or Fenton's reagent were used to compare with the inhibition reactors. Three AFFF concentrations o f 60ppm, 120 ppm and 480 ppm were pretreated with Fenton's reagent. The sixth inhibition test was conducted to investigate the effects o f the defoamer alone on the nitrification process. In this test, two control reactors i.e., no AFFF, no defoamer; two reactors with defoamer alone, and two reactors with 120 ppm AFFF concentration and appropriate dose o f defoamers were used.
The first two inhibition tests that were conducted to evaluate the effect o f defoamers at 60ppm concentrations were conducted for a period of 8 hours with 2 hour cycles o f each anaerobic feed, anaerobic, aerobic and settle period. However the remaining four tests were conducted with one o f the three control and one of the three inhibitions reactors being run for an extended 2 hour aerobic cycle
2.4 Toxicity Pass-Through Testing Toxicity pass-through testing was performed on the inhibition reactors in Phase IB to estimate
the maximum loading of the pretreated AFFF wastewater to the VIP plant without increasing effluent toxicity at the VIP plant. The acute toxicity pass-through tests were conducted using the procedures outlined by the EPA (USEPA, 1989). At the end o f the BNR inhibition batch aeration period, the mixed liquor was allowed to settle and clarified supernatant was decanted from each reactor and filtered through a coarse glass fiber filter. Toxicity samples were submitted to a qualified bioassay laboratory for acute toxicity testing using M. bdhia and C. variegatus following the current EPA procedures.
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3.0 RESULTS 3.1 Reference Reactor Performance
The reference reactor was operated for 16 weeks and monitored for MLSS, SVI, COD, ammonia nitrogen and TKN on a semi-weekly basis (Table 3-1). Collection o f influent and effluent (supernatant) samples and the mixed-liquor allowed calculation o f COD and ammonia removal as well as the food-to-microorganisms (F/M) ratio.
The average COD removal was approximately 95% while removal for ammonia-nitrogen averaged 96%. Throughout Phase IB, the reference reactor exhibited very good nitrification. Even at times when there was a sharp increase in the feed ammonia concentrations, there was no inhibition to nitrification. In Phase IB, the reference reactor operation did not include aeration during the feed cycle. This was determined to be more representative of the VIP BNR process. There were no major operational problems dining this phase and the reactor operated continuously for sixteen weeks without any disruptioa The SVI values o f the reactor were steady, averaging 132 mL/gram and the sludge had good settling characteristics. .
The reactor was also monitored for pH during the different stages o f operation. The pH o f the feed solution was maintained at approximately 6.7 with a bicarbonate alkalinity o f approximately 300-400 mg/1 as calcium carbonate. The pH during the various cycles ranged from 7.5 to 7.8. The alkalinity o f the supernatant was about 100-150 mg/1 as calcium carbonate. Alkalinity o f the feed was sufficient to provide good nitrification throughout this phase o f the study. The dissolved oxygen (DO) concentration was also continuously monitored during the different stages o f the reactor operation. The average DO values ranged between 0.5 to 1.0 mg/L during the feed stage; 0.15 to 0.10 mg/L during the anaerobic stage; and 5.5 to 6.0 mg/L during the aeration stage o f the reactor
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TABLE 3.1 : Weekly Performance of the Reference Reactor during the Phase IB Study
Week MLSS mg/l
1 3020 2560
2 2704 3616
3 4512 4584
4 4844 4716
5 4884 5096
6 4324 4808
7 4904 3976
8 4276 5852
9 5068 5628
10 5488 4984
11 5152 5040
12 4908 5028
13 4976 4408
14 4364 3788
15 4172 4756
16 3360 3760
Average 4 4 8 6
~ : Data not available
MLVSS mg/l
2820 2128 2348 3136 3292 3460 3684 3636 3716 4044 3380
3872 3832 3148 3556 4788 4024 4804 4748 3716 4312 4680 4104 4156 4080 3544 3496 3012 3328 3920 2764 2972
3641
COD mg/l Feed Super.
871 38 932 27 820 40 871 43 748 40 1025 45 1025 63 1025 40 1035 43 968 30 997 43 1020 50 1002 12 1040 20 1012 53 984 32 1009 81 1063 102 1061 63 1104 43 1127 48 1025 61 991 53 1050 66 1020 68 1056 81 1009 61 ~~ ~~ 1053 81 ~~ 991 38
998 51
% COD Ammonia N trogen, mg/l
removal Feed
Supern.
95.7 43.00 97.1 28.20
0.86 ~
95.1 28.20
0.46
95.1 20.90
0.48
94.6 23.10
0.36
95.6 20.60
0.31
93.8 86.20 96.1 113.40
1.92 2.52
95.9 38.30
0.96
96.9 86.60 95.7 30.50
1.53 1.58
95.1 ~
~
98.8 32.90
1.27
98.1 75.70
23.02
94.8 32.20
0.10
96.7 ~
~
92.0 53.70
0.92
90.4 49.60
0.43
94.0 ~
~
96.1 ~
95.8 12.15
0.82
94.1 11.69 94.7 26.70 93.7 ~
0.89 0.10
~
93.3 ~
~
92.3 94.0 20.70
~~ ~ 23.30
0.40 0.20
92,3 ~ 34.60
96.2 ~
0.10
95 41
1
TKN, mg/l
Feed Supern.
170.0 241.0
10.70 ~
147.0 6.50
202.0 7.90
380.0 16.20
338.0 14.40
210.7 28.20
380.6 33.00
636.9 49.70
688.9 26.60
149.3
1.56 ~
132.4 0.84
110.1
1.61
80.7 11.90
~
106.8 3.09
126.6 ~
3.26 ~
~
61.0 0.95
61.0 1.00
25.7 1.00 ~
102.9 ~
39.2 ~
140.7
206
2.20 ~
1.50
1.80 ~
11
SVI mL/gram
68 88 102 76 78 98 114 127 133 137 162 146 153 163 152 120 138
124 146 150 146 149 143 139 131 159 160 172 156 126 149 133
132
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13
operation. The DO was adjusted by changing the flow o f air which was measured with the help of a flow meter, attached to the air supply line.
3.2 Butyl Carbitol Analysis The chromatogram obtained for the butyl carbitol concentrations tested did not show any
prominent peaks with the exception for the chromatograph for 50 ppm AFFF concentration. Two prominent peaks were seen at retention times o f 1.68 minutes and 2.17 minutes. This indicated that the concentrations tested were low enough to be detected however, the butyl carbitol was not 100% pure. A similar prominent peak was seen at 1.68 retention time in chromatograms obtained for the synthetic feed solution. Samples from the control reactors were also measured under the respective peaks which had lower areas under each peak as shown Appendix A. On the other hand prominent peaks with comparatively larger areas were seen in samples from the inhibition reactors at around the same retention time. However, the data was not sufficient enough to determine that these peaks belonged to butyl carbitol or there was any type o f interference because similar type o f peaks were also observed at the same retention time at samples obtained from the control reactors and from the feed solution which did not contain any AFFF.
3.3 BNR Inhibition Batch Assays Pretreated with Defoamers A study that was conducted previously as an independent study in the laboratory tested six
to eight defoamers that were recommended by 3M as possible candidates for curtailing the foaming caused by AFFF. The study consisted of a batch reactor with 4 hours of aeration. A screening analysis was performed and showed that 7.5 milhliters/liter of concentrated defoamer was needed to
14
curtail the foaming attributable to 80 mg/L o f AFFF. In addition, the dosage o f defoamer required to curtail the foaming attributable to 80 mg/L of AFFF exerted a COD o f approximately 4,700 mg/L. It was also observed that even with 7.5 mls/liter of defoamer added to the mixed liquor in the inhibition reactors, foaming increased over the course o f 4 hours o f biological treatment.
The results o f this study showed that some defoamers worked better than others. Based on these results, a defoamer that exhibited the most successful results as well as the defoamer that the Navy is currently using were incorporated in the nitrification inhibition batch assay experiments. Since the Phase 1A results showed excessive foaming at 50 and 60 ppm AFFF, the two defoamers were tested for 60 ppm AFFF concentration where foaming was the most excessive. The data for the inhibition testing with the defoamers are shown in Appendix B.
3.3.1 Pretreatm ent with Defoamer 8710 Triplicate reactors were setup for controls and triplicate reactors were setup for 60 ppm AFFF pretreated with Defoamer 8710 which is currently used by the Navy at Oceana Naval Air Station to suppress the foaming caused by AFFF. The conditions o f this inhibition test are shown in Table 3-2. The results did not show any significant solids washout in both control and inhibition reactors. The ammonia nitrogen removal rates were around 72 percent for the control reactors. However, there was significant nitrification inhibition with 60 ppm AFFF pretreated with Defoamer 8070 as compared to the control reactors as shown in Table 3-3. There was no ammonia removal in all o f the inhibition reactors. Nitrate levels in the effluent were also less than 5 mg/L indicating that not much ammonia was converted to nitrate and lack o f nitrification for the inhibition reactors. However, the operation o f the inhibition reactors were changed from aerated feed cycle to non-aerated feed cycle in Phase
15
US00000973
TABLE 3-2-- BNR Inhibition Reactor 60 ppm AFFF Components Pretreated with Defoamer 8710
PARAMETER
Total Reaction Volume (mL)
Batch MLSS (mg/L)
Batch MLVSS (mg/L)
Seed Biomass Volume (ml)
Effective wastewater (feed & AFFF) Volume, ml
AFFF Concentration (ppm)
AFFF Volume for the simulated wastewater (mL)
Volume of defoamer 8710 added (mL)
Volume o f Synthetic Feed Solution for the simulated wastewater (mL)
CONTROL REACTORS
A, A2 A,
6,000
6,000
6,000
3,320
3,347
3,260
3,047
3,027
2,940
4,000
4,000
4,000
2,000
2,000
2,000
000 0.0 0.0 0.0
000
2,000
2,000
2,000
INHIBITION REACTORS
B, 6,000
B, 6,000
B, 6,000
3,900
3,900
3,713
3,393
3,400
3,233
4,000
4,000
4,000
2,000
2,000
2,000
60 60 60 4.0 4.0 4.0
75 75 75
1,921
1,921
1,921
16
US00000974
TABLE 3-3 -- Nitrification Inhibition at 60 ppm AFFF Pretreated with Defoamer 8070
Reactor
Feedstock
Reference Reactor Decant Control (Al) Control (A2)
Control (A3) AFFF (Bl) AFFF (B2) AFFF (B3)
AFFF llm
0
0
^Initial NH, - N mg/L
30.50
1.58
Final NH,-N mg/L
% Removal NH, -N
^Initial NO, - N mg/L
0.70.
30.1
Final
NO, - N mg/L
Initial COD mg/L
1,190
5.9
Final COD mg/L
COD Removal
%
0 21.25 0 21.25 0 19.62 60 17.41 60 16.73 60 16.73
5.85 6.32 5.20 22.99 22.11 22.11
72.4 70.3 73.5 -32.0 -32.0 -32.0
0.7 23.3 401
32.2 92.0
0.7 25.1 401
58.5 85.4
0.7 25.1 401
32.2 92.0
2.6 3.0 596** 118
80.2
1.2
4.1 596**
116
80.6
0.7
3.2 596**
123
79.3
* Initial values correspond to the measurements taken at the end o f feeding stage, (end o f 2 hours)
** Corresponds to the total COD which includes Reference Reactor Decant = 5.9 mg/L, Feedstock COD = 1190 mg/L, AFFF COD = 1,737 mg/L, and Defoamer 8070 COD = 4930 mg/L.
17
IB. The inhibition test results for Phase IA, showed that there was significant ammonia removal during the initial aerated feed stage. Therefore, the reduction in nitrification may be attributed to the change in the operation mode o f the reactors. The COD removal rates were higher for the control reactors ranging between 85 to 92 percent. For the inhibition reactors, the COD removal rates were significantly lower than the control reactors ranging between 45 to 49 percent. The air supply to each reactor was monitored during the react phase with submergible dissolved oxygen probe to ensure that appropriate amount o f dissolved oxygen was provided.
3.3.2 Pretreatm ent with D efoam er AF9020 Defoamer AF9020 was recommended by the manufacturer during the initial screening period. The results of the initial screening study showed that Defoamer AF9020 had more successful results, therefore it was used in the inhibition tests. The conditions o f the inhibition test are summarized in Table 3-4. During the testing, there was no significant loss in the solids in both control and inhibition reactors. The ammonia nitrogen removal rates were much lower for the inhibition reactors than the controls ranging between 28 to 49 percent indicating nitrification inhibition as shown in Table 3-5. The COD removal rates were also significantly lower for the inhibition reactors (47 to 56 %) when compared to the control reactors (88 to 98%). This inhibition test was also performed under non aerated feed conditions, however, the nitrification potential was better than Defoamer 8710. The oxygen uptake rates were lower for the inhibition reactors when compared to the control reactors as shown in Appendix B.
18
TABLE 3-4-- BNR Inhibition Reactor 60 ppm AFFF Components Pretreated with Defoamer AF9020
PARAMETER
Total Reaction Volume (mL)
Batch MLSS (mg/L)
Batch MLVSS (mg/L)
Seed Biomass Volume (ml)
Effective wastewater (feed & AFFF) Volume, ml
AFFF Concentration (ppm)
AFFF Volume for the simulated wastewater (mL)
Volume o f defoamer AF9020 added (mL)
Volume o f Synthetic Feed Solution for the simulated wastewater (mL)
CONTROL REACTORS
A, 6,000
A2 6,000
A, 6,000
2,993
2,993
2,813
2,667
2,620
2,520
4,000
4,000
4,000
2,000
2,000
2,000
000 0.0 0.0 0.0
000
2,000
2,000
2,000
INHIBITION REACTORS
B, 6,000
b2 6,000
B, 6,000
3,413
3,333
3,620
3,033
2,960
3,213
4,000
4,000
4,000
2,000
2,000
2,000
60 60 60 4.0 4.0 4.0
15 15 15
1,981
1,981
1,981
19
TABLE 3-5 -- Nitrification Inhibition at 60 ppm AFFF Pretreated with Defoamer AF9020
Reactor
Feedstock Reference Reactor Decant Control (Al) Control (A2) Control (A3) AFFF (Bl) AFFF (B2) AFFF (B3)
*Initial AFFF nh3- n ppm mg/L
0 26.70
0 0.10
Final nh3- n mg/L
% "Initial Removal no3- n nh3- n mg/L
0.9
24.3
Final
n o 3- n mg/L
Initial COD mg/L
1,125
104.3
Final COD mg/L
COD
Removal %
0 24.6 0 24.0 0 25.7 60 22.3 60 23.5 60 21.4
2.8 88.6 3.2 86.7 2.8 89.1 11.3 49.3 13.2 44.4 15.4 28.0
0.8 19.0 444.5
10.3 97.7
0.8 22.1 444.5
23.7 94.7
0.8 17.0 444.5
50.6 88.6
0.8 13.5 1,907** 1004
47.3
0.8 9.0 1,907** 830
56.5
0.8 13.8 1,907** 964
49.5
* Initial values correspond to the measurements taken at the end o f feeding stage, (end o f 2 hours)
** Corresponds to the total COD which includes Reference Reactor Decant = 104.3 mg/L, Feedstock COD = 1125 mg/L, AFFF COD = 1,737 mg/L, and Defoamer AF9020 COD = 5,300 mg/L.
US00000978
20
3.3.3 Pretreatment with Best Performing Defoamer Another inhibition test was also performed with the best performing defoamer (Defoamer AF9020) at 120 ppm AFFF concentration. The sequence o f the regular inhibition test was changed with this set. In the previous tests, significant nitrification inhibition was noted in reactors containing AFFF and defoamers. Nitrification was achieved successfully in the control reactors. The nitrification inhibition in the reactors containing AFFF wastewater and defoamer could be attributed both due to the presence of defoamers as well as AFFF. Therefore, this test was conducted using duplicate reactors instead o f triplicates. There was duplicate set o f control with no defoamers or AFFF, one duplicate set with defoamers only, and one duplicate set with both defoamers and 120 ppm AFFF. One of the main reasons for conducting the test in this fashion was to evaluate whether it was AFFF or the defoamer that would contribute to toxicity pass-through during the toxicity testing. The aeration stages o f one o f each set were also extended for additional two hours in order to verify the effects o f additional aeration on improvements in nitrification potential. The test conditions are shown in Table 3-7. The inhibition results are also shown in Table 3-8 with significant improvement in nitrification potential in all of the reactors that had extended aeration during the react stage. For example, ammonia removal rates were greater than 99 percent for the control, 99.5 percent for the reactor with defoamer only, and 99.6 percent for the reactor containing both defoamer and 120 ppm AFFF. The nitrate values were also the highest for all o f the extended aeration reactors indicating good nitrification potential. However, COD reduction did not follow the trend and exhibited no reduction in the reactors containing 120 ppm AFFF. The COD o f the 120 ppm AFFF itself also increased significantly which might have contributed to the low removal rates.
21
US00000979
TABLE 3-6-- BNR Inhibition Reactor 120 ppm AFFF Components Pretreated with Best Performing Defoamer Extended Aeration of React Phase with Defoamer AF9020
PARAMETER
Total Reaction Volume (mL)
Batch MLSS (mg/L)
Batch MLVSS (mg/L)
Seed Biomass Volume (ml)
Effective wastewater (feed & AFFF) Volume, ml
AFFF Concentration (ppm)
AFFF Volume for the simulated wastewater (mL)
Volume of defoamer AF9020 added (mL)
Volume o f Synthetic Feed Solution for the simulated wastewater (mL)
CONTROL REACTORS
A, 6,000
a2 6,000
3,167
3,193
2,767
2,647
4,000
4,000
2,000
2,000
00 0.0 0.0
00
2,000
2,000
DEFOAMER ONLY
B, 6,000
b2 6,000
5,073 4,710
4,480 4,020
4,000 4,000
2,000 2,000
120 ppm A FFF and DEFOAMER
c, 6,000
c2 6,000
4,800
4,960
4,140
4,220
4,000
4,000
2,000
2,000
0 0 120 0.0 0.0 8.0
120 8.0
30 30 30 30
1,970 1,970 1,962
1,962
22
US00000980
TABLE 3-7 -- Nitrification Inhibition at 120 ppm AFFF Pretreated with Best Performing Defoamer Extended Aeration of React Phase with Defoamer AF9020
Reactor
Feedstock
Reference Reactor Decant
Control (Al)
E xtended Aeration Reactor
Control (A2)
Defoamer Only (Bl)
Defoamer Only (B2)
E xtended A eration Reactor
AFFF (Cl)
AFFF (C2)
E xtended Aeration Reactor
AFFF ppm
"Initial NHj - N
mg/L
0 32.2
0 0.10
Final NH,-N
mg/L
% "Initial Removal NOj-N NHj - N mg/L
1.1
17.7
Final NOj-N
mg/L
Initial COD mg/L
1,102
38.4
Final COD mg/L
0 26.3
0 23.3 0 20.7 0 19.9
120 20.7 120 19.9
0.01 99.9
2.2 90.6 2.9 86.0 0.1 99.5
6.6 68.1 0.07 99.6
0.9 31.1 393
38.4
0.9 15.7 393
115.3
0.9 21.3 1276 615
1.0 27.7 1,276 692
0.9 18.5 2,408** 2,486 0.9 28.2 2,408** 2,588
COD Removal
%
90.2 70.7 51.8 45.8 -3.2 -7.5
* Initial values correspond to the measurements taken at the end o f feeding stage, (end o f 2 hours)
** Corresponds to the total COD which includes Reference Reactor Decant = 38.4 mg/L, Feedstock COD = 1,102 mg/L, AFFF COD = 3,396 mg/L, and Defoamer AF9020 COD = 5,300 mg/L.
23
3.4 BNR Inhibition Batch Assays Pretreated with Fenton's Reagent In this part o f the phase IB study, the nitrification inhibition potential o f AFFF pretreated with
Fenton's Reagent were evaluated. The AFFF:H20 2:Fe2+ ratios that were required to achieve appreciable reductions in foaming and AFFF concentrations were determined. Since the direct measurement o f AFFF was difficult and was not a possible dosing parameter at that point, the study assessed the required C0D:H20 2:Fe2+ratio needed to achieve appreciable reductions in foaming and the COD o f the wastewater containing AFFF. Three different concentrations o f wastewater containing 60 ppm, 120 ppm and 480 ppm AFFF were pretreated with Fenton's reagent and the data are attached in Appendix C.
3.4.1 Pretreatm ent with F enton's Reagent a t 60 ppm AFFF The wastewater containing 60 ppm AFFF was pretreated with Fenton's reagent 24 hours prior to the inhibition test. The test conditions with the concentrations o f iron and hydrogen peroxide are shown in Table 3-8. There was no significant loss o f mixed liquor solids from the reactor. The inhibition test results are tabulated in Table 3-9. At 60 ppm AFFF, the ammonia removal rates for the extended aeration inhibition reactors (99.4%) were as high as the control reactors (99.5%). The ammonia nitrogen removal rates for the inhibition reactors operating in non-extended aeration mode were only 10 percent lower than the control reactors. Nitrate concentration in the extended aeration inhibition reactor was higher than the control reactor operating under similar conditions which indicated good nitrification potential. The COD removal rates were greater than 89 percent in all o f the reactors indicating the strong oxidation potential o f Fenton's reagent.
24
TABLE 3-8-- BNR Inhibition Reactor 60 ppm AFFF Components Pretreated with Fenton's Reagent
PARAMETER
Total Reaction Volume (mL)
Batch MLSS (mg/L)
Batch MLVSS (mg/L)
Seed Biomass Volume (ml)
Effective wastewater (feed & AFFF) Volume, ml
AFFF Concentration (ppm)
AFFF Volume for the simulated wastewater (mL)
Fe2+Concentration (mg/L) Fenton's Reagent
H20 2 Concentration (mg/L) Fenton's Reagent
CONTROL REACTORS
A, 6,000
A2 6,000
A, 6,000
2,793
2,893
2,807
2,480
2,460
2,433
4,000
4,000
4,000
2,000
2,000
2,000
INHIBITION REACTORS
B, B, B,
6,000
6,000
6,000
2,880
2,760
2,740
2,880
2,367
2,460
4,000
4,000
4,000
2,000
2,000
2,000
0 0 0 120 120 120 0.0 0.0 0.0 8.0 8.0 8.0
0 0 0 300 300 300
0 0 0 3,000 3,000 3,000
25
TABLE 3-9 -- Nitrification Inhibition at 60 ppm AFFF Pretreated with Fenton's Reagent
Reactor
Feedstock
Reference Reactor Decant
Control (Al)
Extended Aeration
Control (A2)
Control (A3)
AFFF (Bl)
Extended Aeration
AFFF (B2)
AFFF (B3)
AFFF ppm
0
0
"Initial NH, - N mg/L
20.7
0.4
Final % "Initial NH, - N Removal NO, - N mg/L NH, - N mg/L
0.8
14.8
Final NO, - N
mg/L
Initial COD mg/L
973.5
53.0
0 20.7
0 20.7 0 19.9 60 16.3
60 16.3 60 16.3
0.1 99.5
5.3 74.4 4.9 75.4 0.1 99.4
6.0 63.2 6.5 60.1
0.8 33.8
360
0.8 17.1
360
0.8 16.9 360
0.8 37.4 846**
0.8 N/A 0.8 N/A
846 ** 846**
Final COD mg/L
COD
Removal %
26.3 92.6
26.5 92.6 13.3 96.3 92.9 89.0
79.6 90.6 79.6 90.6
* Initial values correspond to the measurements taken at the end o f feeding stage, (end o f 2 hours)
** Corresponds to the total COD which includes Reference Reactor Decant = 53 mg/L, Feedstock COD = 973.5 mg/L, Fenton's Treated AFFF COD = 1,459 mg/L.
c 26 aOO>
o o o
CD 00
3.4.2 Pretreatm ent with F enton's Reagent at 120 ppm AFFF The reactor components for this inhibition test are shown in Table 3-10. There was little or no solids loss in inhibition reactors when compared to the control reactors. The results showed no nitrification inhibition for the reactors operating in extended aeration mode for both controls and inhibition sets. The ammonia removal rates were 99.6 percent for both sets and nitrate production rates were high in both inhibition and control reactors indicating good nitrification as shown in Table 3-11. The COD removal rates were not as high for the inhibition reactors as compared to the control reactors in both non-extended and extended aeration operation modes possibly due to the higher COD o f the pretreated AFFF.
3.4.3 Pretreatm ent with F enton's Reagent at 480ppm AFFF The concentration o f AFFF was increased to 480 ppm to determine if there was significant inhibition in nitrification after Fenton's oxidation as pretreatment. The test conditions are shown in Table 3-12. There was no significant loss in the solid concentrations between the control reactors and inhibition reactors. The inhibition test results showed that nitrification potential decreased by 50 percent even in the inhibition reactor operating in extended aeration mode when compared to the control reactor with similar operational mode. Even though in the remaining control reactors the ammonia removal rates were not as high (ranging from 64 to 67%), the ammonia removal in the inhibition reactors were still 50 percent less than that o f control reactors which indicated nitrification inhibition (Table 3-13). The COD removal rates for the inhibition reactors decreased significantly at all operational modes when compared to the control reactors. The COD o f 480 ppm AFFF w astew ater pretreated with Fenton's reagent was 9,000 mg/L which contributed greatly to the
27
TABLE 3-10-- BNR Inhibition Reactor 120 ppm AFFF Components Pretreated with Fenton's Reagent
PARAMETER
Total Reaction Volume (mL)
Batch MLSS (mg/L)
Batch MLVSS (mg/L)
Seed Biomass Volume (ml)
Effective wastewater (feed & AFFF) Volume, ml
AFFF Concentration (ppm)
AFFF Volume for the simulated wastewater (mL)
Fe2+ Concentration (mg/L) Fenton's Reagent
H20 2 Concentration (mg/L) Fenton's Reagent
CONTROL REACTORS
A, 6,000
A2 6,000
A, 6,000
2,973
3,040
2,927
2,627
2,727
2,640
4,000
4,000
4,000
2,000
2,000
2,000
000 0.0 0.0 0.0
000
000
INHIBITION REACTORS
B, 6,000
b2 6,000
B, 6,000
2,900
2,940
2,800
2,647
2,653
2,473
4,000
4,000
4,000
2,000
2,000
2,000
60 60 60 4.0 4.0 4.0
300 300 300
3,000
3,000
3,000
28
US00000986
TABLE 3-11 -- Nitrification Inhibition at 120 ppm AFFF Pretreated with Fenton's Reagent
Reactor
Feedstock
Reference Reactor Decant
Control (Al)
Extended Aeration
Control (A2)
Control (A3)
AFFF (Bl)
Extended Aeration
AFFF (B2)
AFFF (B3)
AFFF ppm
0
0
"Initial NH3-N
mg/L
34.6
0.1
Final NHj-N
mg/L
% Removal NHj-N
"Initial NO,-N
mg/L
0.9
17.4
Final NO,-N
mg/L
Initial COD mg/L
1,082
54.1
0 25.2 0.1 99.6 1.0 30.4
397
0 26.3 0.5 98.1
1.0 19.0
397
0 27.3 0.5 98.2 0.9 15.9 397
120 27.3 0.1 99.6 0.9 31.0 1,276**
120 42.6
12.3 71.1
0.9
28.6 1,276**
120 56.6
9.8 82.7
1.0 28.4 1,276**
Final COD mg/L
13.5 13.5 13.5 338 325 284
COD Removal
%
96.6 96.6 96.6 73.5 74.6 77.7
* Initial values correspond to the measurements taken at the end o f feeding stage, (end o f 2 hours)
** Corresponds to the total COD which includes Reference Reactor Decant = 54.1 mg/L, Feedstock COD = 1,082 m/L, Fenton's Treated AFFF COD = 2,638 mg/L.
29
TABLE 3-12-- BNR Inhibition Reactor 480 ppm AFFF Components Pretreated with Fenton's Reagent
PARAMETER
Total Reaction Volume (mL)
Batch MLSS (mg/L)
Batch MLVSS (mg/L)
Seed Biomass Volume (ml)
Effective wastewater (feed & AFFF) Volume, ml
AFFF Concentration (ppm)
AFFF Volume for the simulated wastewater (mL)
Fe2+Concentration (mg/L) Fenton's Reagent
H20 2 Concentration (mg/L) Fenton's Reagent
CONTROL REACTORS
A, A2 A3
6 ,0 0 0
2,667 2,440 4,000
6 ,0 0 0
2,593 2,413 4,000
6 ,0 0 0
2,587 2,380 4,000
2 ,0 0 0
2 ,0 0 0
2 ,0 0 0
0 0 .0
0 0 .0
0 0 .0
00 0
00 0
INHIBITION REACTORS
B, b 2 B3
6 ,0 0 0
2,153 1,927 4,000
6 ,0 0 0
2,347 2,073 4,000
6 ,0 0 0
2,493 2,253 4,000
2 ,0 0 0
2 ,0 0 0
2 ,0 0 0
480 480 480 32 32 32
300 300 300
3,000
3,000
3,000
30
US00000988
TABLE 3-13 -- Nitrification Inhibition at 480 ppm AFFF Pretreated with Fenton's Reagent
Reactor
Feedstock
Reference Reactor Decant
Control (Al)
Extended Aeration
Control (A2)
Control (A3)
AFFF (Bl)
Extended Aeration
AFFF (B2)
AFFF (B3)
AFFF ppm
0
0
` Initial NHj-N mg/L
23.3
0.2
Final NHj-N mg/L
% `Initial Removal no3- n NH,-N mg/L
0.9
3.4
Final NOj - N
mg/L
Initial COD mg/L
1,038
13.1
0 18.3 0.1 99.5
1.1 30.7
346
0 17.6 5.8 67.0 0.9 13.9
346
0 17.6 6.2 64.8 0.9 15.0 346
480 15.0
6.8 54.7
0.8
17.0 3,352"
480 15.0 9.3 38.0 0.8 11.2 3,352" 480 14.4 9.7 32.6 0.9 14.0 3,352"
Final COD mg/L
COD Removal
%
26.3
39.4 13.1 2,575
2,680 2,706
92.4
88.6 96.2 23.2
20.0 19.3
* Initial values correspond to the measurements taken at the end o f feeding stage, (end of 2 hours)
** Corresponds to the total COD which includes Reference Reactor Decant =13.1 mg/L, Feedstock COD = 1,038 m/L, Fenton's Treated AFFF COD = 9,012 mg/L.
31
organic strength o f the wastewater. The COD removal rates for the inhibition reactors ranged between 19 to 23 percent indicating biological degradation inhibition as well.
3.5 Toxicity Pass-Through Testing The results o f effluent toxicity pass-through tests conducted with the mysid shrimp and
sheepshead minnow are shown in Table 3-14. In the samples in which AFFF was treated with the defoamers, the minnows were less sensitive to the effluent and exhibited toxicity only in one sample with 120 ppm AFFF pretreated with the best performing defoamer. However, the mysids were much more sensitive and the effluent exhibited toxicity in all o f the inhibition reactors pretreated with the tw o types o f defoamers. The results showed that Defoamer 8710 LCS0values (65 to 72%) were higher than the defoamer AF9020 (LC^'s between 45-50%) indicating the organisms' less sensitivity to the defoamer that is currently used by NAS Oceana. The tests conducted in duplicates showed that there was no pass-through toxicity in the reactors that had defoamer only, however, pass-through toxicity was observed to both minnows and mysid shrimp in the reactors containing AFFF + Defoamer AF9020. This indicated the possibility that the toxic component o f the effluent was AFFF rather than the defoamers.
For the inhibition reactors containing AFFF pretreated with the Fenton's reagent, there was no pass-through toxicity to minnows in all o f the pretreatment scenarios tested. However, toxicity to mysid shrimp was evident in all of the inhibition reactors pretreated with Fenton's reagent. As the AFFF concentrations increased, the LC50levels decreased indicating increased levels o f toxicity. In all of the test conditions, the feedstock was toxic to both minnows and mysid shrimp most possibly due to the presence o f high ammonia concentrations.
32
TABLE 3-14. Summary of Toxicity Test Data for Inhibition Tests
Pretreatment Operation
60 ppm AFFF + Defoamer 8710
60 ppm AFFF + Defoamer AF9020
120 ppm AFFF + Best Performing Defoamer (AF9020)
60 ppm AFFF + Fenton's Reagent
120 ppm AFFF + Fenton's Reagent
480 ppm AFFF + Fenton's Reagent
Toxicity Bioassay Test Date
Sample Type
Sept/30/97 Nov/3/97 Dec/11/97 Nov/18/97 Dec/2/97 Nov/25/97
Feedstock R.R. Mixed Liquor Control A1 Control A2 Control A3 Inhibition B1 Inhibition B2 Inhibition B3
Feedstock R.R. Mixed Liquor Control A1 Control A2 Control A3 Inhibition B1 Inhibition B2 Inhibition B3
Feedstock R.R. Mixed Liquor Control A1 Control A2 Control B1 Defoamer Only Control B2 Defoamer Only Inhibition Cl (AFFF+Def.) Inhibition C2 (AFFF+Def.)
Feedstock R.R. Mixed Liquor Control A1 Control A2 Control A3 Inhibition B1 Inhibition B2 Inhibition B3
Feedstock R.R. Mixed Liquor Control A1 Control A2 Control A3 Inhibition B1 Inhibition B2 Inhibition B3
Feedstock R.R. Mixed Liquor Control A1 Control A2 Control A3 Inhibition B1 Inhibition B2 Inhibition B3
LCjo
Sheepshead Mysid
Minnows
Shrimp
3.5 8.1 >100 >100 >100 >100 >100 >100 >100 >100 >100 72 >100 66 >100 65
19.7 >100 >100 >100 >100 >100 >100 >100
42 >100 >100 >100 >100 >100 >100 22.5
36.6 >100 >100 >100 >100 >100 >100 >100
30.6 >100 >100 >100 >100 >100 >100 >100
13.8 >100 >100 >100 >100 >100 >100 >100
12.5 >100 >100 >100 >100 50.5
50 45
33 >100 >100 >100 >100 >100 43.2 40.6
23.3 >100 >100 >100 >100 >100 93.9 75.2
39.2 >100 >100 >100 >100 40.2 63.9 66.0
9.7 >100 >100 >100 >100 >100 42.1 69.1
33
US00000991
4.0 SUMMARY and CONCLUSIONS The pretreatment results with defoamers demonstrated that effluent ammonia nitrogen concentrations for Defoamer #8710 were higher (22-23 mg/L) than the effluent ammonia levels for the Defoamer AF 9020 (11.3 to 15.4 mg/L) indicating a better pretreatment and less nitrification inhibition for the latter defoamer (Figure 4-1). The nitrate production rates were in accordance with the ammonia removal rates, and were also demonstrated with excellent mass balances on the nitrogen species. In Phase I, at 60 ppm AFFF, the effluent ammonia levels were less than 1 mg/L indicating no nitrification inhibition. It should be noted that the aeration during the feed stage in Phase II was terminated to better simulate the VIP BNR operational conditions. The higher effluent ammonia levels in Phase II was most probably due to termination o f aeration during the feed cycle o f the inhibition reactors. In order to verily the effect o f the additional aeration to nitrification, another inhibition test was conducted with 120 mg/L AFFF and the best performing defoamer (AF 9020). In this test the aeration stage o f one o f the triplicate inhibition and control reactors were extended for additional two hours. The effluent ammonia nitrogen levels were less than 0.1 mg/L for both controls and the inhibition reactors as exhibited in Figure 4-2. In all o f the inhibition tests performed with the defoamers as pretreatment compounds, the COD removal rates decreased significantly in the inhibition reactors when compared to the control reactors with no defoamer or AFFF added. Even though the test conducted with the best performing defoamer under extended aeration operational mode showed some improvement in the COD removal rate, the reduction was only 50 percent when compared to the 90 percent reduction observed in the control reactor. Fenton's reagent was used to pretreat AFFF at concentrations 60 ppm, 120 ppm and 480 ppm. There were no nitrification inhibition at 60 ppm and 120 ppm AFFF wastewater pretreated with
34
AFFF Pretreated with Defoamers
Time, hr
Def #8710, Control Def AF9020, Control
Def #8710
Def AF9020
Figure 4-1. AFFF Pretreated with Defoamers (60 ppm AFFF)
35
AFFF Pretreated with Defoamers (120 ppm AFFF) Extended Aeration Test Results
Time, hr
Figure 4-2. AFFF Pretreated with Defoamers under Extended Aeration Mode of Operation (120 ppm AFFF) 36
US00000994
the Fenton's reagent as compared to the controls. Oxidation with Fenton's reagent was more effective than the defoamers used in pretreating AFFF. The nitrification inhibition potential decreased at concentrations greater than 60 ppm however, nitrification inhibition occurred at 480 ppm AFFF pretreated with Fenton's reagent. Figure 4-3 shows that the ammonia concentrations in the effluent were higher for 120 and 480 ppm AFFF wdthout the extended aeration phase. However, the nitrification inhibition was significantly evident wdth extended aeration period at 480 ppm AFFF as shown in Figure 4-4.
The toxicity test results showed that, with the exception o f one case, there was no pass through toxicity to the sheepshead minnows in all both o f the pretreatment options used in the inhibition tests. However, the effluent from the inhibition reactors were consistently toxic to mysid shrimp in all o f the pretreatment options that included defoamers and Fenton's reagent. Toxicity was observed at 60 ppm AFFF concentration as well as 480 ppm AFFF concentration. The LC50results did not exhibit any particular trends with increasing AFFF concentrations as well. In addition, the feedstock exhibited toxicity to both test organisms in all o f the inhibition tests conducted due to the presence o f high ammonia concentrations.
The analytical measurements for determination o f butyl carbitol component o f AFFF did not distinguish major prominent peaks in the chromatograms obtained from the ion chromatograph. The data was not sufficient enough to show a distinction between the control reactors and reactors containing AFFF.
The AFFF-laden wastewater pretreated with defoamers and Fenton's reagent did not show any nitrification inhibition at concentrations as high as 120 ppm AFFF. However, nitrification inhibition was evident at 480 ppm AFFF wastewater pretreated with Fenton's reagent.
37
US00000995
AFFF Pretreated with Fenton's Reagent
246
8
Time, hr
Control F480 Control F120 Control F60
F480
F120
F60
Figure 4-3. AFFF Pretreated with Fenton's Reagent
38
US00000996
AFFF Pretreated with Fenton's Reagent Extended Aeration
30
2 4 6 8 10 Time, hr
Figure 4-4. AFFF Pretreated with Fenton's Reagent (Extended Aeratiom Test Results) 39
US00000997
In all o f the pretreatment alternatives used, the inhibition reactor effluents exhibited pass through toxicity to mysid shrimp at AFFF concentrations 60 ppm or greater, whereas the effluent was not toxic to the sheepshead minnows.
In summary, there is not an advantage to pretreating AFFF wastewater prior to discharge to a nitrifying plant because although pretreatment with defoamers or Fenton's reagent increases the inhibition concentration, it causes pass-through toxicity. The only time it may be used is if the nitrifying plant has mixing zone (i.e., acute toxicity limit o f <1.0 Toxic Units). This would allow AFFF to be discharged at higher concentrations without causing pass-through toxicity.
.40 US00000998
References
1. Military Specification Mil-F-24385F
2. CHjM Hill (1992). "Wastewater Effluent Pilot Study for the Advanced Fire Fighting Training Facility." Naval Facilities Engineering Command, Atlantic Division, Naval Contract
N62470-91- R-6650.
3. CH2M Hill (1995). "Wastewater Treatability Final Report for the Advanced Fire Fighting Training Facility." Naval Facilities Engineering Command, Atlantic Division, Navy Contract N62470-91- C-6650.
4. Chan, D. B., (1978). " Disposal o f Wastewater Containing Aqueous Film Forming Foam." Technical Memorandum No. M-54-78-06, Civil Engineering Laboratory, Naval Construction Battalion Center, Port Hueneme, California.
5. Chan, D.B., and Bingham, P., (1988). `AFFF-laden Wastewater Treatment Technology Initiation Decision Report (DDR)." Technical Memorandum No. TM-71-88-11, Civil Engineering Laboratory, Naval Construction Battalion Center, Port Hueneme, California.
6. Thomas, J.F., and LeFebvre, E.E., (1973). "Biodegradability and Toxicity o f FC-200 Aqueous Film Forming Foam." Report No. EHL(K) 74-3, USAF Environmental Health Laboratory, Kelly AFB, Texas. 41
US00000999
7. Saam, R., and Rakowski, P. (1979). "Firefighting School Wastewater Study." Technical Memorandum No. 54-79-14, Civil Engineering Laboratory, Naval Construction Battalion Center, Port Hueneme, California.
8. Saam, R., Rakowski, P, and Aydlett, G., (1979). "Treatability o f Firefighting School Wastewaters: U.S. Navy Compliance with POTW Pretreatment Requirements." Proceedings o f the 34thPurdue Industrial Waste Conference, West Lafayette, Indiana.
9. Union Carbide (1978). "Unox System Treatability Study Report, U.S. Navy Firefighting School." Naval Facilities Engineering Command, Atlantic Division, Norfolk, Virginia.
10. "Toxicity o f Selected Effluents from the U.S. Navy Firefighting School, Norfolk, VA to Embryos o f Eastern Oysters." Technical Report by, Atlantic Division, Naval Facilities Engineering Command, Norfolk, Virginia.
11. LeFebvre, E.E. (1971). "Biodegradability and Toxicity o f Light Water." Report No. EHL (K) 71-36, USAF Environmental Health Laboratory.
12. LeFebvre, E.E., and Thomas, J.F. (1973). "Biodegradability and Toxicity o f AER-O-Water 3% and 6% Aqueous Film Forming Foam." Report No. EHL(K) 73-22, USAF Environmental Health Laboratory, Kelly AFB, Texas.
, 42
US00001000
13. LeFebvre, E.E., and Inmand, R.C. (1974). "Biodegradability and Toxicity o f Light water FC-206,, Aqueous Film Forming Foam." Report No. EHL(K) 74-26, USAF Environmental Health Laboratory, Kelly AFB, Texas.
14. LeFebvre, E.E., and Inmand, R.C. (1975). "Biodegradability and Toxicity o f ANSUL K74100, Aqueous Film Forming Foam." Report No. EHL(K) 75-3, USAF Environmental Health Laboratory, Kelly AFB, Texas.
15. Grace and Associates Inc., (1986). "Engineering Investigation o f Impact o f AFFF on Wastewater Treatment Performance at Naval Air Station, Memphis, Millington, Tennessee." U.S. Navy Contract N62467-86-C-0351, Naval Facilities Engineering Command, Atlantic Division, Norfolk, Virginia.
16. Engineering-Science Incorporated (1986). "Physical-Chemical Treatment from Navy Firefighting Schools." Naval Facilities Engineering Command, Atlantic Division, Contract No. N00025-74-C-0004, Norfolk, Virginia.
17. Bowers, A R., Eckenfelder, W.W. Jr., Gaddipati, P., and Monsen, R.M., (1989). "Treatment o f Toxic or Refractory Wastewater with Hydrogen Peroxide." Water Science Technol,. 21, p.477.
43
US00001001
Barber, M., Miner, C., Pelizzetti, E., Borgarello, E., and Serpone, N., (1987). "Chemical Degradation o f Chlorophenols with Fenton's Reagent." Chemosphere, 16:2225-2237.
Erten-Unal, M., Paranjape, S., Schafran, G.C., and Wiliams, F.W., "Evaluation o f the Effects o f AFFF Inputs to VIP Biological Nutrient Removal Process and Pass-Through ToxicityPhase IA",technical report, Naval Research Laboratory, NRL/MR/6180-98-8141, February 1998, 46pp.
United States Environmental Protection Agency (1979). "Methods o f Chemical Analysis o f Water and Wastes." Environmental Monitoring and Support Laboratory, EPA 600/4-79-020, Cincinnati, Ohio.
American Public Health Association (1995). "Standard Methods for the Examination of W ater and Wastewater." 19th Edition, Washington, DC.
W alt, R. J., Udell, M. D. and Leung, S. W. "Treatment o f Contaminated Soils Using Catalyzed Hydrogen Peroxide", Proceedings o f the First International Symposium, Chemical Oxidation: Technology for the Nineties, Vanderbilt University, Nashville, Tn, 1992.
United States Environmental Protection Agency (1989). "Toxicity Reduction Evaluation Protocol for Municipal Wastewater Treatment Plants."
44
APPENDIX A AFFF Butyl Carbitol Analysis
A -l
US00001003
US00001004
fa rm 1 " -1 Sample
Dionex Chromatography DataBase Alcohol standards
Record
Analytes and Concentrations
1. Ethanol, 2. 1-Propanol, 3. 2-methyl-2-propene-1-oi, 4. Cyclopentanol, 5.
Phenylmethanol, 6. 1-Phenylethanol. 7. 3-Phenyl-1-propanol, 8.
2-Ethyl-1-hexanol, 9. 1-Decanol, 10. 1-Undecanol, 11. Dodecanol 12
1-Tridecanol, 13. 1-Tetradecanol
'
100
Columns No. 1 2 PCR
OmniPac PCX-500
Sample Vol
Mobile Phases & Postcolumn Reagents ;90/10 Acetonitrile/Water
;Water
0.3 M NaOH...................
25 pL
Tim , %i : %2 .
.00' 20- 80-
' ' ' .o-: " 20. " ` oi
5.00 95-
5-
14.00. 95.
5.
rj
' %4 r .
Sample Dilution & Prep, Comments
Injection @ 0 : 1 minutes. Column equilibrated to initial conditions for at least 7 minutes before injection.
Flow Rates: Mobile Phase
1 mUmin Regen
mL/min
Detector and Settings PAD (Au), E1 = 0.10V (720ms), E2 = 1.00V (120ms), E3=-0.80V (300ms)
Reagent
Notes Optional columns for this application are OmniPac PAX-500 and lonPac NS1 10 pm.
1 mUmin
Reference Slide 5084-01
H ie s re su lts are intended as a starting point for m ethods developm ent a n d a re provided to show feasibility. A -3
US00001005
Monoalcohols with Pulsed Electrochemical Detection
10 Minutes
15
20
Peaks: 1. Ethanol 2 . 1- Propanol 3. Methallyl alcohol 4. Cyclopentanol 5. Phenylmelhanol 6 . 1- Phenylelhanol 7. 3-PhenyM-propanol 3 .2- Ethyl-1 -hexanol 9 . 1- Decanol 10.1- Undecanol 11.1- Dodecanol 12.1- Trldecanol 13.1- Tetradecanol
5064*01
APPENDIX B BNR Inhibition Batch Assays Pretreated with Defoamers
B -l
US00001007
B-2
US00001008
*********************
AUTOMATIC CALIBRATION UPDATE
**************+ ****** 1
Data File : c:\peaknet\data\afff0008.DXD
Sample Name: Autocal 2-lppm
-
I n j e c t if : 8
M e t h o d F i l e : c : \ p e a k n e t \ m e t h o d \ a f f f .m e t System Name: D X-500
C a l . Level : 2
Report Date Collected Vial # Last Update Detector Analyst
11/2/97 9:07:01 PM 11/2/97 8:38:40 PM
11/2/97 8:38:32 PM ED4 0
* * * * * * * * * * * * * *******
COMPONENTS FOUND IN THIS RUN
********* ******** * ** * *
COMP NUM
COMPONENT NAME
OLD MEASURED
NEW
RET.TIME RET.TIME RET.TIME
OLD
MEASURED
RESPONSE RESPONSE
NEW RESPONSE
Data File Sample Name I n j e c t ff Method File System Name Column Type Data Points Module Name
C:\peaknet\data\afff0008.DXD Autocal 2-lppm 8
c :\ p e a k n e t \ m e t h o d \ a f f f .m e t D X - 500
1200
I D :24 0 a 8 5
Report Date Collected Vial a Calibrated Detector Operator Rate Moduleware
11/2/97 9:07:01 PM 11/2/97 8:38:40 PM
11/2/97 9:07:01 PM ED4 0
1.00 Hz 1.17
Calibration Volume
Dilution Start
S t o p Area Reject Pk. Width T h r e s h o l d
External
1
* * * ********** *********
1 Component
0.00 19.98 Report: All
1C00
10.00
19.21
Components ********** *************
Pk. N'um
Ret Component Time Name
Concentrt ion
Height
Area B l . %Delca Code
0.00 butylcarb
0.000
0 0 0 0.00
Totals
0.000
00
*************************
: R e p o r t : U n k n o w n P e a k s ** * * * * * * * * * * * * * * * * * * * * * *
Pk. Num
Ret Comp Time Name
1 1.37 2 2.18 3 13.70 4 14.08 5 14.48 6 16.00 7 18.35
Concentration
.
0.000 0.000 0.000 0.000 0.000 0.000 . 0.000
Height
11804 33138 13711 14174 13376 47029
9733
Area B l . %Delta Code
56265 782834 185872 185381 127915 6262576 351092
1 1 1 1 1 1 1
Totals
0.000
143015
7951935
Schedule: \peaknet\schedule\afff11 2.sch
Relase 4.30
--
11/2/97 9:07:02 PM
B-3
US00001009
File: afff0008.DXD Sample Autocal 2-1ppm
100 1: 80 I 60 ! 40 ;
nC 2D! 1 2 0 - ___ ..
-20 -40 '
6
7
0 2 4 6 8 10 12 14 16 18 20 Minutes
Schedule : \peaknet\schedule\afffll_2.sch Release 4.30
B-4
11/2/97 9:07:02 PM US00001010
*********************
AUTOMATIC CALIBRATION UPDATE
************-*** +'** +*
Data File : c:\peaknet\data\afff0009.DXD
Sample Name: Autocal 3-10ppm
Inject 8
:9
M e t h o d File: c : \ p e a k n e t \ m e t h o d \ a f f f .met
System Name: DX-500
C a l . Level : 3
Report Date Collected Vial S Last Update Detector Analyst
11/2/97 9:35:30 PM 11/2/97 9:07:09 PM
11/2/97 9:07:01 PM ED4 0
************* *******
C O M P O N E N T S FOUND IN THI S R U N
********* ******** *****
COMP NUM
COMPONENT NAME
OLD ' MEASURED
NEW
RET.TIME RET.TIME RET.TIME
OLD
MEASURED
RESPONSE RESPONSE
NEW RESPONSE
Data File Sample Name Inject a Method File System Name Column Type Data Points Module Name
c:\peaknet\data\afff0009.DXD Autocal 3-10ppm 9
C:\peaknet\method\afff.met DX-500
1200
I D :24 0a 85
Report Date Collected Vial a Calibrated Detector Operator Rate Moduleware
11/2/97 9:35:30 PM 11/2/97 9:07:09 PM
11/2/97 9:35:30 PM ED4 0
1.00 Hz 1 .17
Calibration Volume
Dilution Start
Stop Area Reject P k . Width Thr eshold
External
1
1 0.00 19.98
1000
10.00
19.21
*** ** ****** *********** Component Report: All Components ********** ** * ** ********
Pk . Num
Ret Component Time Name
C o ncent ra tion
Height
A r e a Bl . % D e l ta Code
0 0.00 butylcarb
0.000 -
0
0 n 0.00
Totals
0.000
00
*********** *********** *** Peak Report: Unknown Peaks *** ********** ** * * * ********
Pk. Num
Ret Component Time Name
1.15 2 1.63 3 2.1-7 4 14.50 5 14.90 6 15.70 7 17.87
Concentration
0.000 0.000 0.000 0.000 0.000 0.000 0.000
Height
16763 12772 57222 17590 15712 66779
8401
Area Bl . %Delta Code
174443 109527 608242 241420 186309 2046738 146694
1 l' 1 T
l 2
1
Totals
0.000
195240
3513373
Schedule: \peaknet\schedule\afff11 2.sch
Release 4.30
11/2/97 9:35:30 PM
B-5
US00001011
File: afff0009.DXD Sample Autocal 3-10ppm
100 i 80 i
40 i
-40 ' 2 4 6 8 10 12 14 16 18 20 Minutes
Schedule : \peaknet\schedule\afffll_2.sch
Release 4.30
:
B-6
1 1 / 2/91 9 : 3 5 : 3 1 PM
ki
US00001012
*********************
AUTOMATIC CALIBRATION UPDATE
**************** +t:ltii<
Data File Sample Name Inject # Method File System Name C a l . Level
c :\peaknet\data\afff0010.DXD Autocal 4-50ppm 10 c:\peaknet\method\afff.met DX-S00 4
Report Date: 11/2/97
Collected : 11/2/97
Vial #
:
Last Update: 11/2/97
D e t e c t o r .: E D 4 0
Analyst
:
10 :04 :00 PM 9:35:38 PM
9:35:30 PM
********************
COMPONENTS FOUND IN THIS RUN
***********************
COMP NUM
COMPONENT NAME
OLD MEASURED
NEW
RET.TIME RET.TIME RET.TIME
OLD
MEASURED
RESPONSE RESPONSE
NEW RESPONSE
Data File Sample Name Inject n Method File System Name Column Type Data Points Module Name
c :\peaknet\data\aff f0010 .DXD Autocal 4-50ppm 10 c : \ p e a k n e t \ m e t h o d \ a f f f .m e t DX - 500
1200
I D :24 O a 85
Report Date Collected Vial # Calibrated Detector Operator Rate Moduleware
11/2/97 10:04:00 PM 11/2/97 9:35:38 PM
11/2/97 10:04:00 PM ED4 0
1.00 Hz 1.17 -
Calibration Volume
Dilution Start
S t o p Area Reject Pk. W i d t h T h r e s h o l d
Ixternal
1
1 0.00 19.98
1000
10.00
19.21
* * * + * * * * * * * * * * * * * * * * C o m p o n e n t R e p o r t : A l l C o m p o n e n t s + + + + + + + + ** + * * * * * * *
Pk. Num
Ret Component Time Name
Concentration
Height
Area B 1 . %Delta Code
0 0.00 butylcarb
0.000
0 0 0 0.00
Totals
0.000
00
************************ Peak Report: Unknown Peaks **************************
Pk. Num
Ret Component Time Name
1 0.20 2 0.55 3 0.88 4 1.68 5 2.17 S 2.63 7 2.80 8 13.58 9 13.98 10 14.47 11 15.18
Concentration
*
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Height
17700 15933 13966 253910 86432 52031 70379 13995 19065
7367 82451
rea B 1 . VDelta Code
101128 209209 318774 2050260 639595 252956 560109 192785 245079 100201 2829753
1 2 2 2 1
2 2 1 1 2
i
Schedule: \ p e a k n e t \ s c h e d u l e \ a f f f 11 2.sch
Release 4.30
"
11/2/97 10:04:00 PM
B-7
US00001013
Totals
0.000
633229
7499848
File: afffOOIO.DXD Sample Autocal 4-50ppm
100 ,
80 : 60 40
11 8910
-20
-40
0 2 4 6 8 10 12 14 16 18 20 . Minutes .
Schedule: \peaknet\schedule\afffll 2.sch Release 4.3 0
B-8
11/2/97 10:04:00 PM
)
|
US00001014
Data File : c :\peaknet\data\afff0011.DXD
Sample Name : Feedstock
Inject #
: 11
M e t h o d F i l e : c : \ p e a k n e t \ m e t h o d \ a f f f .m e t
System Name: D X - 500
Column Type :
Data Points: 1200
Module Name :
I D :24 0a 85
Report Date Collected Vial # Calibrated Detector Operator Rate Moduleware
11/2/97 10:32:28 PM 1 1 / 2 / 9 7 10 :04 :08 P M
11/2/97 ED4 0
o o o o
PM
1.00 Hz 1.17
Calibration Volume
Dilution Start
Stop Area Reject P k . Width Threshold
External
1
1 0.00 19.98
1000
10.00
19.21
********************** Component Report : All Components it************** ** ****
Pk. Num
Ret Component Time Name
Concentration
Height
A r e a 31. oD e I t a Code
, 0 0.00 butylcarb
0.000
0. 0 0 0 . 0 0
Totals
0.000
0
0
* * * * * * * * * * * * * * * * * * * * * * * * * p e a k R e p o r t : U n k n o w n P e a k s ** * * * * * * * * * * * * * * * * * * * * * *
Pk . Num
Ret Component Time Name
Concentration
Height
1 0.37 2 1.50 3 1.70 4 3 .23 5 3 .57 6 12.92 7 14.35 8 14.75 9 15.15 10 16 .90 21 1 9 . 1 2
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 . 0.000 0.000
6079 174280
78894 47648 38382
5671 16647 25643 20530 54468 17625
Totals
0.000
485866
Area Bl. %Delta Code
73315 503133 2767642 977080 1749473
71319 234728 309572 257484 6735289 226412
)
2 2 2 2 1 1
1 2 2
1
13905446
S c h e d u l e : \ p e a k n e t \ s c h e d u l e \ a f f f 11 2 . s c h
Release 4.30
~
B-9
11/2/97 10:32:23 ?M
US00001015
File: afffOOl 1.DXD Sample Feedstock 100 .
80 | 60 i 40 ; nC 20 1
0--
-20 -40
0 2 4 6 8 10 12 14 16 18 20 Minutes
S c h e d u l e : \peaknet\schedule\afffll_2.sch Release 4.30
B-10
1 1 / 2 / 9 7 1 0 : 3 2 : 2 8 PM
'f
US00001016
Data File
c :\peaknet\data\afff0012.DXD
Sample Name: R.R.Supernatant
Inject #
: 12
M e t h o d F i l e : c : \ p e a k n e t \ m e t h o d \ a f f f .m e t
System Name: DX-500
Column Type:
D a t a P o i n t s : 1200
Module Name:
ID :24 0 a 85
Report Date Collected Vial # Calibrated Detector Operator Rate Moduleware
1 1 / 2 / 9 7 1 1 : 0 0 : 5 4 Pf 1 1 / 2 / 9 7 1 0 : 3 2 : 3 6 Pf
1 1 / 2 / 9 7 1 0 : 0 4 : 0 0 Pf ED4 0
l'.OO H z 1.17
Calibration Volume
Dilution Start
Stop Area Reject P k . Width Threshold
External
1
1 0.00 19.98
1000
10.00
19.21
*********** *********** Component Report : All Components *********************
Pk. Nunn
0
Ret Component Time Name
0.00 butyicarb
Concentration ' 0.000
Height 0
Ar e a 31. %Delta Code
0 0 0.00
Totals
0.000
0
0
************************* Peak Report: Unknown Peaks **************************
Pk . Num
Ret Component Time Name
1 0.93 2 1.53 3 1.75 4 2 .b7 5 18.52
Concentrt ion
0.000 0.000 0.000 0.000 0.000
Height
5135 65168 97372 42968 42 378
Area Bi . Code
110929 497555 701056 447030 9958336
1 1 1 1 1
Totals
O.CO
253021
11714906
S c h e d u l e : \ p e a k n e t \ s c h e d u l e \ a f f f l l _ 2 .s c h Release 4.30
B -ll
11/2/97 11:00:54 PM
US00001017
File: afff0012.DXD Sample R.R.Supernatant
100 j 80 { 60 | . 40 nC 20 i 2? 4
0 ---- -/A
-40
6 8 10 12 14 16 18 20
Minutes
Schedule: \peaknet\schedule\afffll 2.scu
Release 4.30
B-12
11/2/97 11:00:54 PM
US00001018
Data File Sample Name: Inject # Method File: System Name: Column Type: Data Points: Module Name:
c :\peaknet\data\afff0013.DXD Al end of feed--control 13
c :\peaknet\method\afff.met DX-500
1200
I D :24 0 a 85
Report Date Collected Vial # Calibrated Detector Operator Rate Moduleware
11/2/97 11:29:19 PM~' 11/2/97 11:01:02 PM
11/2/97 10:04:00 PM ED4 0
1.00 Hz 1.17
Calibration Volume
Dilution Start
Stop Area Reject Pk. Width Threshold
External
1
1 0.00 19.98
1000
10.00
19.21
************* ********* Component Report: All Components ***********************
?k. Num
Ret Component Time Name
Concentration
Height
Area B 1 . %Delta Code
0 0.C0 butylcarb
0.000 0 0 0 0.00
Totals
0.000
0
0
************ ************* Peak Rep o r t . unknown
************ **************
?k. Num
Ret Component Time Name
1 1 .35 2 1 .b U 3 2 .10 4 3.37
5 7.77
g 14 .40 7 14.87
8 15.27
9 ib .6b 10 17.4 0
11 1 8 . 9 2
Concentration
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Height
8247 93738 36379 17276
9051 10905 26453 20568 95874 22380 19295
A r e a 31. % D e l ta Code
60027 972378 737607 868206 101992
68482 356033 258871 5219791 1386225 519742
1
2 2 1 1 2 2 2 2 1
Totals
0.000
360166
10549353
S c h e d u l e : \ p e a k n e t \ s c h e d u l e \ a f f f 1 1 _ 2 .s c h
Release 4.30
~ .* '
B-13
l'l/2/97 11:29:19 PM
US00001019
File: afff0013.DXD Sample A1 end of feed-control
100 :
80 |
60 !
40 |
nC 20 :
0-
-20 :
23 ?
6 78
10
-40 :
11
0 2 4 6 8 10 12 14 16 18 20 Minutes
Schedule: \peaknet\schedule\afff11_2.sen Release 4.30
B-14
11/2/S7 11:29:20 ?M
1
US00001020
Data File : c :\peaknet\data\afff0014.DXD
Sample Name : A2 end of feed--control
Inject #
: 14
M e t h o d File: c : \ p e a k n e t \ m e t h o d \ a f f f .met
System Name : D X -500
Column Type :
Data Points: 1200 Module Name:
I D :24 0a 85
Report Date Collected Vial # Calibrated Detector Operator Rate Moduleware
11/2/97 11:57:47 PM 11/2/97 11:29:28 PM
11/2/97 10:04:00
ED4 0
' 1.00 1.17
Hz
PM
C a l i b r a t i o n V o l u m e D i l u t i o n S t a r t S t o p A r e a R e j e c t Pk. W i d t h T h r e s h o l d
External
1
1 0.00
19.98
1000
10.00
19.21
********************** Component Report: All Components ***************-********
Pk. Num
Ret Component Time Name
0.00 butvlcarb
Concentration
Totals
0.000 0.000
Height
Area B l . %Delta Code
0 0 0.00
0
************************* Peak Report : Unknown Peaks **************************
Pk. Num
Ret Component Time Name
i 1.57
2 2.00 2.73
d 3.03 5 7.05 6 10.75 7 14.03 8 14.43 9 16.92 10 13.42
Concentration
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Height
103565 43305 17326 28232 4724 33995 19348 15740 30076 34818
Area Bl. ! Code
1078567 605094 140831 338452 54002
4383015 236017 189493
6191445 1652544
1 1 2 2 1 1 1 1 1 1
Totals
0.000
331131
14869459
S c h e d u l e : \ p e a k n e t \ s c h e d u l e \ a f f f 11 2.sch
Release 4.30
7
B-15
1 1 / 2 / 9 7 1 1 : 5 7 : 4 7 ...
US00001021
File: afff0014.DXD Sample A2 end of feed-control
100 ,
j
80 | 60 ; 40
nC 20 . ] 2 34
-20 ~ -40
0 2 4 6 8 10 12 14 16 18 20 Minutes
S c h e d u l e : \peaknet\schedule\afff11_2.sch
Release 4.30
"
B-16
11/2/97 11:57:48 PM US00001022
Data File Samp le Name Inject # Method File System Name Column Type Data Points Module Name =---- -------- =
c :\peaknet\data\afff0015.DXD A3 end of feed--control 15
Report Date Collected Vial #
11/3/97 12:26:15 AM 11/2/97 11:57:56 PM
c : \ p e a k n e t \ m e t h o d \ a f ff .met D X - 500
Calibrated Detector
11/2/97 10:04:00 PM ED40
Operator
1200
Rate
1.00 Hz
ID:24 0a 85 M o d u l e w a r e
1.17
======================= ============
II II
II II II II II II II II It
II II II II II II II II II
II II II II II II II II
II
II II
lalibration Volume
Dilution Start
Stop Area Reject Pk. W i dth Threshold
External
1
1 0.00 19.98
1000
10.00
19.21
* * * * fc******^ * * * * * * * * * C o m p o n e n t Report: A l l C o m p o n e n t s * * * * * * * * * * * * * * * * * * * * * *
?!< . Num
Ret Component Time Name
_
Concentration
Height
A r e a Bl. % Delta
. Code
0 0.00 butylcarb
0.000 0 0 0 0.00
Totals
0.000
0
0
** ** * * * * * * * * * * * * * * * * * * * * peak Report: Unknown Peaks **** *********************
?k. Num
Ret Component Time Name
1 1 .62 2 2.15 3 3.12 4 3.32 5 8.93 6 14.68 7 15.08 8 15.48 Q 17.25 10 18.78
Concentration
0.000 0.000 0.000 0.000 0.000 0.000 o.coo 0.000 0.000 0.000
Height
'97035 34952 42930 48690 48685 17855 15788 11909 44837 23020
A r e a Bl. % Delta Code
1022349 779163
2157887 1283012 3596865
246147 193096 157173 6514876 900380
1
1 2 2 1 2 2 1 1
1
Totals
0.000
385700
16850947
S c h e d u l e : \ p e a k n e t \ s c h e d u l e \ a f f f l l _ 2 .s c h
Release 4.30
'
B -17
11/3/97 12:26:15 AM
US00001023
File: afff0015.DXD Sample A3 end of feed-control
100 , i
80 |
i
60 !
40
-40
; ; 1 I i ! : ' i ; i I I : I i I : | ...T j ! ' i | I ! i I j . I ----------,
0 2 4 6 8 10 12 14 16 18 20 Minutes
Schedule: \peaknet\schedule\afff11_2.sen Release 4.30
. Mt*!**
B-18
.-
11/3/97 12:26:15 AM
v
US00001024
Data File : c:\peaknet\data\afff0016.DXD Sample Name : B1 end of e e d--60ppm
Inject #
.- 16
M e t h o d F i l e : c : \ p e a k n e t \ m e t h o d \ a f f f .m e t
System Name: DX-500
Column Type :
Data Points : 1200
Module Name:
I D :24 0a 8 5
Report Date Collected Vial # Calibrated Detector Operator Rate Moduleware
11/3/97 12:54:44 AM 11/3/97 12:26:24 AM
1 1 / 2 / 9 7 10 :04 :0 0 ED4 0
-
1.00 Hz 1.17
PM
Calibration Volume
Dilution Start Stop Area Reject P k . Width Threshold
External
1
l 0.00
19.98
,
1000
10.00
19.21
* * * * * * * * * * * * * * * * * * * * * * C o m p o n e n t R e p o r t : A l l C o m p o n e n t s * * * * * * * * * * * * **********
Pk. Num
Ret Component Time Name
Concentration
Height
Area Bl. % Delta Code
0 0.00 butylcarb
0.000 0 0 0 0.00
Totals
0.000
0
0
* * * * * * * * * * * * * * * * * * * * * * * * * Pggj R e p o r t : U n k n o w n P e a k s * * * * * * * * * * * * * * * * * * * * * * * *
Pk. Num
Ret Component Time Name
1 1.58 2 1.97 3 3.02 4 14.67 5 15.07 6 17.18
Concentration
0.000 0 .o co 0.000 0.000 0.000 0.000
Height
599958 47856 41021 14640 16736 27874
A r e a 31 . % Del ta Code
3384904 726511
2497587 189454 196379
4714611 .
1 1 1
i 1 1
Totals
0.000
748084
12209545
9
S c h e d u l e : \ p e a k n e t \ s c h e d u l e \ a f f f l l _ 2 .s c h
Release 4.30
:
B-19
11/3/97 12:54:44 AM
US00001025
100
,File:
I
afff0016.DXD
Sample
B1
end
of
feed--60ppm
60 ! 40 :
-40
0 2 4 6 8 10 12 14 16 18 20 Minutes
Schedule : \peaknet\schedule\afff11_2 .sen
Release 4.30
"-
B-20
11/3/97 12:54:44 AM
V
US00001026
Data File Sample Name Inject # Method File System Name Column Type Data Points Module Name
c : \ p e a k n e t \ d a t a \ a f f f 0 0 1 7 .D X D B2 end of feed--60ppm 17 c : \ p e a k n e t \ m e t h o d \ a f f f .met DX-500
1200
I D :24 0 a 85
Report Date Collected Vial # Calibrated Detector Operator Rate Moduleware
11/3/97 1:23:12 AM 11/3/97 12:54:52 AM
11/2/97 10:04:00 PM ED4 0
1.00 Hz 1.17
Calibration Volume
Dilution Start
Stop Area Reject Pk.-Width Threshold
External
1
1 0.00 19.98
1000
10.00
19.21
********************** Component Report: All Components ***********************
Pk. Num
.8
Ret Component Time Name
Concentration
4.75 butylcarb
0.254
Totals
0.254
Height
5765 5755
Area Bl . %Delta Code
40702
1 4 .78
40702
***+******* ************** peak Report: Unknown Peaks ************ **************
Pk . Num
Ret Component Time Name
1 1.27 2 1.58 3 1.97 4 2.55 5 2.93 6 3.38 7 3.68 9 ' 8.00 10 14.02 11 14.57 12 14.88 13 16. 8 5 14 1 8 . 2 7
Concentration
0.000 0.000 0.000 0.000 0.000 ' 0.0.00 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Height
Area ' 31. %Delta Code
14662
581472
680245
4335773
54000
868450
12032
109646
22045
491915
25104
268361
22462
704569
37231
1925693
9589
142353
16345
226292
20145
242418
149913
11721359
89369 . 10323213
1 2 2 1 2 2 2 1 1 2 2 2 2
Totals
0.000
1*153145 3 2 041513
S c h e d u l e : \ p e a k n e t \ s c h e d u l e \ a f f f 1 1 _ 2 .s c h
Release 4.30
"
B-21
11/3/97 1:23:12 AM
US00001027
File: afff0017.DXD Sample B2 end of feed--60ppm
100 ,
80 |
60 I
40 i nC 20
0
'Is 4 5 6y 1!h ! 1 ! o
13
-20
-40
14
0 10 12 14 16 18 20
Minutes
<
Schedule: \peaknet\schedule\afff11_2.sch
Release 4.30
B-22
11/3/97 1:23:13 AM
\
US00001028
Data File : c:\peaknet\data\afff0018.DXD
Sample Name : B3 end of eed--60ppm
Inject #
: 18
M e t h o d F i l e : c : \ p e a k n e t \ m e t h o d \ a f f f .m e t
S ystem Name: D X - 500
Column Type :
Data Points : 1200
Module Name:
I D :24 Oa 85
Report Date Collected Vial # Calibrated Detector Operator Rate Moduleware
11/3/97 1:51:41 AM 11/3/97 1:23:21 AM
11/2/97 10:04:00 PM ED4 0
1.00 Hz 1.17
Calibration Volume
Dilution Start
Stop A r e a R e j e c t Pk. W i d t h T h r e s h o l d
External
1
1 0.00 19.98
1000
10.00
19.21
********************** Component Report: All Components ***********************
Pk. Mura
Ret Component Time Name
Concentration
Height
Area B 1 . %Delta Code
0 0.00 butylcarb
0.000
0 0 0 0.00
Totals
0.000
0 0,
* * * * * * * * * * * * * * * * * * * * * * * * * p03.k R e p o r t U n k n o w n P e a k s * * * * * * * * * * * * * * * * * * * * * * * * * *
Pk. Num
Ret Component Time Name
Concentration
Height
Area B 1 . Code
1 0.10 2 1.58 3 1.92 4 2.50 5 2.80 6 2.97 7 3.12 8 3.40 9 4.02 10 7.83 11 1 4 . 7 3 12 1 5 . 1 3 13 1 5 . 5 3 14 1 6 . 9 2
0.000 0.000 0.000 0.000 . 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
15252 688472
55558 20793 46017 33714 15128 24785 10703 20808 13638 18990 14156 30482
85527 4204967
593758 124110 372540 272112 117603
73735 213414 1779034 180974 227420 159182 2522942
1 1 1 2 2 2 2 1 1 1 1 1 1 1
Totals
0.000
1008497
10927318
S c h e d u l e : \ p e a k n e t \ s c h e d u l e \ a f f f 1 1 _ 2 .s c h
Release 4.30
'
.
B-23
11/3/97 1:51:41 AM
US00001029
File: afff0018.DXD Sample B3 end offeed-60ppm
100 ,
'
80 f i!
60 I
40 i
nC 14
1123
-40
:
0
::!
2
.'
4
i ' { ; s I * { ; f : Y i " r ~ f \ ~ T . i -i : | ! . : ! ; ! !
6 8 10 12 14 16 18
Minutes
;}
20
Schedule: \peaknet\schedule\afff1 1 _ 2 .sch
Release 4.30
-"
B-24
i
11/3/97 1:51:42 AM US00001030
: .~S$
Data File : c :\peakhet\data\afff0020.DXD
Sample Name: Al end of anaerobic--control
Inject #
: 20
M e t h o d File: c : \ p e a k n e t \ m e t h o d \ a f f f .met
System Name: DX-500
Column Type:
Data Points: 1200
Module Name:
I D :24 O a 8 5
Report Date Collected Vial # Calibrated Detector Operator Rate Moduleware
11/3/97 2:48:39 AM 11/3/97 2:20:19 AM
11/2/97 10:04:00 PM ED4 0
1.00 Hz 1.17
Calibration Volume
Dilution Start
S t o p A r e a R e j e c t Pk. W i d t h T h r e s h o l d
External
1
l 0.00 19.98
1000.
10.00
19.21
********************** Component Report: All Components ***********************
Pk. Num
0
Ret Component Time Name
Concentration
0.00 butylcarb
0.000
Totals
0.000
Height
0 0
Area B 1 . %Delta Code
0 0 0.00
0
**** + * * * * * * * * * * * * * * * * * * p e a k R e p o r t : U n k n o w n P e a k s * * * * * * * * * * * * * * * * * * * * * * * *
Pk. Num
Ret Component Time Name
Concentration
Height
Area B 1 . %Delta Code
1 1.57 2 2.05 3 2.87 4 3.08 5 3.53 6 3.78 7 7.20 8 14.75 9 15 .15 10 15 .55 11 18.42 12 19.4 0
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
107804 56142 53976 31952 1413 0 9442 58407 15246 23603 19094
102176 50038
1018325 1379145
658026 362311
67279 244421 3385224 211561 301924 262880 18070125 1473832
1 2 2 2 1 1 1 1 2 2 2 2
Totals
0.000
542011 *
27435053
S c h e d u l e : \ p e a k n e t \ s c h e d u l e \ a f f f l l _ 2 .s c h
Release 4.30
'
B-25
11/3/97 2:48:39 AM
US00001031
File: afff0020.DXD Sample A1 end of anaerobic--control
, 100
80 I 60 40
0 2 4 6 8 10 12 14 16 18 20 Minutes
Schedule: \peaknet\schedule\afff11_2.sch Release 4.30
B-26
11/3/97 2:48:39 AM US00001032
Data File : c:\peaknet\data\afff0022.DXD
Sample Name: A3 end of anaerobi c--control
Inject #
22
M e t h o d F i l e : c :\peaknet\method\afff.met
System Name : DX-500
Column Type :
Data Points: 1200
Module Name:
ID :24 Oa 85
Report Date Collected Vial #
Calibrated Detector Operator Rate
Moduleware :
11/3/97 3:45:37 AM 11/3/97 3:17:15 AM
31/2/97 10:04:00 PM ED4 0
1.00 Hz 1.17
Calibration Volume
Dilution Start
Stop A r e a Reject Pk. Wid t h Threshold
Externai
i
1000
10.00
19.21
********************** Component Report: All Components ***********************
Num 0
? s t CornP o n e n t Time Name
Concentration
Height
0.00 butylcarb Totals
0^000"
_
0.000
_0 0
Area Bl. %Delta Code
0 0 0.00 o
* * * * * * * * * * * * * * * * *********
Pk . Num
Ret Comp Time Name
1 1 . 5 5 2 1.95 3 2.98 4 9.08 5 14.53 6 15.02 7 15.42 8 16.90
Peak Report: Unknown
Concentration
0 . 000 0 . 000 0 . 000 0 . 000 0.000 0.000 0 . 000 0.000
P e a k s * * * * * * * * * * * ******* +******)
Height
78653 54075 64556 25865 12794 27662 20097 40144
Area Bl. Delta Code
724030 847460 4327621 1740498
77652 347964 259181 3951285
1 1 1 1 1 1 1 1
Totals
0.000
323848
12275691
Schedule: \peaknet\schedule\aff f n 2 sch
Release 4.30
'
B-27
11/3/97 3:45:37 AM
US00001033
00 File: afff0022.DXD Sample A3 end o f anaerobic-control
I
I
80 i j
60 I
4$
Schedule: \peaknet\schedule\afff1 1 2 sch
Release 4.30
-
B-28
11/3/97 3:45:37 AM US00001034
Data File : c:\peaknet\data\afff0023.DXD
Sample Name : Bl end of anaerobic--60ppm
Inj ect #
: 23
Method File : c:\peaknet\method\afff.met
S y s t e m Name : D X - 500
Column Type :
Data Points: 1200
Module Name :
I D :24 0 a 8 5
Report Date Collected Vial # Calibrated Detector Operator Rate Moduleware
11/3/97 4:14:07 AM 11/3/97 3:45:45 AM
1 1 / 2 / 9 7 10 :04 :00 P M ED4.0
1.00 Hz 1.17
Calibration Volume Dilution Start Stop Area Reject P k . Width Threshold
External
1
1 0.00 19.98
1000
10.00
19.21
********************** component Report: All Components ***********************
Pk. Num
6
Ret Component Time Name
Concentration
4.60 butylcarb
16.877
Totals
16.877
Height
148491 148491
Area B l . %Delta Code
2703590
2 1.47
2703590
************************* Peak Report: Unknown Peaks ***************************
P k .
Ret Component
Num
Time Name
1 1.57 2 1.92 3 3.02 4 3 .80 5 4 .15 7 4.92 8 5.05 9 5.50 10 5 .97 11 6.40 12 6.73 13 6 . 8 7 14 7 . 2 8 15 7.63 15 8 . 1 0 17 3.6 7 13 9 .'02 19 9.50 20 10. 0 3 21 10. 6 0 22 1 3 . 5 5 23 1 3 . 7 5
.
Concentration
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.00-0 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Height
581871 63479 52869 28595
253137 52122 93788
189897 231093 297997 287624 143608 480202 ,329003 306287 336787 265706 . 237106 102103
86651 225350
62931
Area Bl . Code
3677722 967984
2184917 232682
4146095 243409 574691
2199456 2152332 2892131 2022509 1183580 4308352 4413465 4268348 4146135 4013997 3936456 1050331 1726012
644911 .1222559
1 1 2 2 2 2 2 1 1 1 2 2 1 1 1 1 1 1 1 1 1 1
Totals
0.000
4708205
52208071
Schedule: \peaknet\schedule\afff11_2.sch
Release 4.30
'
.
B-29
11/3/97 4:14:07 AM
US00001035
I File: afff0023.DXD Sample B1 end of anaerobic--60ppm
100
22
~iij
-40 : '': `' !
0 2 4 6 8 io 12 14 16 . 18 20
Minutes
Schedule : \peaknet\schedule\afff11_2.sch Release 4.30
.
B-30
11/3/97 4:14:07 AM US00001036
Data File Sample Name Inject # Method File System Name Column Type Data Points Module Name
c:\peaknet\data\afff0024.DXD B2 end of a n a e r o b i c - - 6 0 p p m 24
c :\peaknet\method\afff.met D X - 500
1200
I D :24 0a 85
Report Date Collected Vial # Calibrated Detector Operator Rate Moduleware
11/3/97 4:42:37 AM 11/3/97 4:14:15 AM
11/2/97 10:04:00 PM
ED40
"`
1.00 Hz 1.17
Calibration Volume
Dilution Start
St o p A r e a Rej e c t Pk. W i d t h Thres h o l d
External
l
1 0.00 19.98
1000
10.00
19.21
********************** Component Report: All Components ***********************
Pk. Num
0
Ret Component Time Name
0.00 butylcarb
Concentration 0.000
Height 0
A r e a Bl. %Delta Code
0 0 0.00
Totals
0.000
00
* * * * * * * * * * * * * * * * * * * * * * * * * peaj R e p 0 r t ; U n k n o w n P e a k s * * * * * * * * * * * * * * * * * * * * * * * * * *
Pk. Num
Ret Component Time Name
1 6.93 2 13.33
Concentration
0.000 0.000
Height
18791 30180
Area B l . %Delta Code
664023 416574
l 1
Totals
0.000
48971
1080597
.
File: afff0024.DXD Sample B2 end of anaerobic--60ppm 100
80 ;
60 I
40 i 5
nC 20!
:
,2
:I
-20 i
-40 !
! ' : : ' 1 i i i 1 i i i 1 j 1 1 1 1 1 1 1 1 1 1 1 1 i 1 1 l i I I j I I i i M i11"r j i i i i j
o 2-4
68
1 0 12 14 16 18 20
Minutes
Schedule: \peaknet\schedule\afff11 2.sch
Release 4.30
11/3/97 4:42:37 AM
B-31
US00001037
t
File: afff0030.DXD Sample B1 end of aerobic--60pm
100 3
-20 j -40 ;
4 6 8 10 12 14 16 18 20
Minutes
Schedule: \peaknet\schedule\afff11 2.sch Release 4.30
B-32
11/3/97 10:58:50 AM US00001038
I
Data File Sample Name Inject # Method File System Name Column Type Data Points Module Name
c -.\ p e a k n e t \ d a t a \ a f f f 0 0 3 1 . D X D B2 end of aerobic--60ppm 31 c : \ p e a k n e t \ m e t h o d \ a f f f.met D X -500
1200
ID :24 0a 85
Report Date Collected Vial # Calibrated Detector Operator Rate Moduleware
11/3/97 11:27:16 AM 11/3/97 10:58:58 AM
11/2/97 10:04:00 PM ED4.0
1.00 Hz 1.17
Calibration Volume
Dilution Start
Stop A r e a R e j e c t Pk. W i d t h T h r e s h o l d
External
1
1 0.00 19.98
1000
10.00
19.21
********************** Component Report: All Components ***********************
Pk. Num
Ret Component Time Name
Concentration
Height
Area B 1 . %Delta ' Code
0 0.00 butylcarb Totals
0.000 0.000
0. 0
0 0 0.00 0
************************* Peak Report: Unknown Peaks **************************
Pk . Num
Ret Component Time Name
Concentration
1 1.62 2 1.88 3 2.20 4 3.07 5 12.90 6 13.30 7 13.70 8 16.50 9 16.92 10 18.30
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Totals
0.000
Height
608805 9111
38757 82525 22774 11214 17321
9773 19228 . 17322
836829
Area B1 . Code
3635766 69686
,352877 4680119
301826 87067
209009 230808 196443 1125172
1 1 1 1 1 1 1 2 2 1
10888773
Schedule: \peaknet\schedule\afffll_2.sch Release 4.30
B-33
11/3/97 11:27:17 AM
US00001039
File: afff0031.DXD Sample B2 end of aerobic--60ppm
100 ,
ii
80 !
*
60 ;
40 I
nC 20 0
-20
-40
8 9 10
i ;
567 '''
0246
10 12 14 16 18 20 Minutes
I
Schedule: \peaknet\schedule\afffli 2.sch
Release 4.30
--
B-34
11/3/97 11:27:17 AM US00001040
Data File : c : \peaknet\data\afff0 0 3 2 .DXD
Sample Name: B3 end of aerobic--60ppm
Inject #
: 32
M e t h o d F i l e : c : \ p e a k n e t \ m e t h o d \ a f f f .m e t
System Name : D X - 500
Column Type :
Data Points: 1200
Module Name:
I D :24 0 a 85
Report Date Collected Vial # Calibrated Detector Operator Rate Moduleware
11/3/97 11:55:45 AM 11/3/97 11:27:26 AM
11/2/97 10:04:00 PM E D 4.0
1.00 Hz 1.17
Calibration Volume
Dilution Start
Stop Area Reject P k . Width Threshold
External
1
1 0.00 19.98
1000 ' 10.00
19.21
** ** **** +************ Component Report: All Components * ********************
Pk. Num
Ret Component Time Name
Concentration
Height
Area B . %Delca Code
0 0.00 butylcarb
0. OO O
0
0 0 0.00
Totals
0.000
0
0
* * * ** ****** * * * * * * * * * * * *** Peak Report: Unknown Peaks *** * * * * * * * * * * * * * * * * * * * * *
Pk. Num
Ret Component Time Name
Concentration
1 1.62 2 1.83 3 2.15 4 2.40
5 2.80 6 3.38 1 3.53 8 3.77 9 8.30 10 12.82 11 1 3 . 2 7 12 13.67 13 1 5 . 4 7 14 1 6 . 8 7 15 17.73
0.000 0.000 0 .O OO 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Totals
0.000
Height
696627 18940 71116 16053 19373 18555 15620 20749 4765 15549 22219 19694 25359
, 22190 4021
990830
Area B I . %Delta Code
400924 5 171438 552582 112345 159557 103199 93018
1301455 231369 228173 287466 248433
3572524 351648 48742
2
2 2 2 1
2 2 1 i 1 1 2 2
I
1
11471394
S c h e d u l e : \ p e a k n e t \ s c h e d u l e \ a f f f 11 2 . s c h
Release 4.30
'3
B-35
11/3/97 11:55:45 AM
US00001041
File: afff0032.DXD Sample B3 end of aerobic--60ppm 100
80
60
40 : nC 20
o -20
!j
;^34 5 67g
' " :X
13 14 15
101112 ,
! i ; /.
-- - -j"
-40
i ! i ! ; ! : i T' i i
'!..r'"i r i" "; i - ; " : - : ...r ..r
8 10 12 14 16 18
Minutes
20
Schedule : \peaknet\schedule\afff11_2.sch Release 4.30
B-36
11/3/97 11:55:46 AM US00001042
Data File : c:\peaknet\data\afff0033 .DXD
Sample Name: STANDARD 3-10ppm
Inject #
: 33
.
M e t h o d F i l e : c :\ p e a k n e t \ m e t h o d \ a f f f .m e t
System Name: DX-500
Column Type:
Data Points: 1200
,
Module Name:
I D :24 0 a 8 5
Report Date Collected Vial # Calibrated Detector Operator Rate Moduleware
11/3/97 12:24:12 PM 11/3/97 11:55:53 AM
11/2/97 10:04:00 PM ED4 0
1.00 Hz 1.17
C a l i b r a t i o n V o l u m e D i l u t i o n S t a r t S t o p A r e a R e j e c t Pk. W i d t h T h r e s h o l d
External
1
1 0.00 19.98
1000
10.00
19.21
********************** Component Report: All Components ********************** *
Pk. Num
Ret Component Time Name
Concentration
Height
A r e a B 1 .% D e l t a Code
0 0.00 butylcarb
0.000
0 0 0 0.00
Totals
0.000
00
* * * * * * * * * * * * * * * * * * * * * * * * * P e a k R e p o r t : U n k n o w n P e a k s **** + * * * * * * + + + + + + * * * * + * + **
Pk. Num
Ret Component Time Name
Concentration
1 1.88 2 2.28 3 2.62 4 3.42 5 9.80 6 12.68 7 13.08 8 13.47 9 14.10 10 14.43 11 17.22
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 ' 0.000
Totals
0.000
Height
10969 11441 95822 15775
9689 19614 24681 18537 107278 44220 13815
371840 s
Area B1 . Code
85747 74416 538273 657240 1170617 289698 33146 222960 1969890 983595 307906
1 1 1 1 1 1 1 1 2 ,2 1
6632187
S c h e d u l e : \ p e a k n e t \ s c h e d u l e \ a f f f l l _ 2 .s c h Release 4.30
B-37
11/3/97 12:24:12 PM
US00001043
File: afff0033.DXD Sample STANDARD 3-10ppm
100 _
80 ;
60 ;
40 ;
nC 20 :
0r -20 :
-40
12i
910
6 78
~~L/
11
10 12 14 is 18 20
Minutes
Schedule: \peaknet\schedule\afffll 2.sch Release 4.30
B-38
11/3/97 12:24:12 PM US00001044
Data File Sample Name Inject # Method File System Name Column Type Data Points Module Name
c :\peaknet\data\afff0034.DXD
Al end of settle--control
34
c :\peaknet\method\aff f.met
D X-500
.
1200
Report Date Collected Vial # Calibrated Detector Operator Rate Moduleware
11/3/97 12:52:40 PM 1 1 / 3 / 9 7 1 2 : 2 4 :2 0 P M
1 1 / 2 / 9 7 10 :04 :00 P M ED4 0
1.00 Hz 1.17
II II II II II II II II II II II II II II II II II II Il M II o II fO ii ^ ii II o Il (D II II 00 ll tn
Calibration Volume
Dilution Start
S t o p A r e a R e j e c t Pk. W i d t h T h r e s h o l d
External
1
1 0.00 19.98
1000
10.00
* * * * * * * * * * * * * * * * * * * * * * Component R e p o r t : All Components ****
19.21 ******
Pk. Num
Ret Component Time Name
Concentration
Height
Area B 1 . %Delta Code
0 0.00 butylcarb
0.000 0 0 0 0.00
Totals
0.000
0
0
************************* peak Report: Unknown Peaks **************************
Pk . Num
Ret Component Time Name
1 1.60 2 1.87 3 2.25 4 2.55 5 3.07 6 7.98 7 12.63 8 13.03 9 13.43 10 1 3 . 8 3 11 15.78 12 16.08 13 1 6 . 5 5 14 1 7 . 3 2
Concentration
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Totals
0.000
Height
70406 33000 39588 47346 102020
3715 13830 25097 23100 11414 120144 102650 81308 36177 i
709796
Area Bl. Code
669638 434800 574462 677110 7075674
37783 200863 339764 311820
84491 9602106 2542203 3056759
651916
2 2 2 2 2 1 1 2 2 1 2 2 2 2
26259389
Schedule : \peaknet\schedule\afff11_2.sch
Release 4.30
'!
B-39
11/3/97 12:52:41 PM
US00001045
File: afff0034.DXD Sample A1 end of settle-control
100
80 60 40 !
[ ''
.
0 2 4 6 8 10 12 14 16 . 18 20
Minutes
Schedule: \peaknet\schedule\afff11_2.sch
Release 4.30
.
11/3/97 12:52:41 PM
B-40
US00001046
Data File Sample Name Inject # Method File System Name Column Type Data Points Module Name
C : \ p e a k n e t \ d a t a \ a f f f 0 0 3 5 .DXD
A2 end.of settle- -control
35
c:\peaknet\method\afff.met
DX-500
.
1200
I D :24 0a 85
Report Date Collected Vial # Calibrated Detector Operator Rate Moduleware
11/3/97 1:21:11 PM 1 1 / 3 / 9 7 12 :S 2 :4 8 P M
11/2/97 10:04:00 PM ED4 0
1.00 Hz 1.17
Calibration Volume
Dilution Start
Stop A r e a R e j e c t Pk. W i d t h T h r e s h o l d
External
1
1 0.00 19.98
1000
10.00
19.21
* * * * * * * * * * * * * * * * * * * * * * Component Report: All Components ***********************
Pk. Num
Ret Component Time Name
Concentration
Height
Area B 1 . %Delta Code
0 0.00 butylcarb
0.000 0 0 0 0.00
Totals
0.000
0
0
**+****** + * * * ********** Peak Report: Unknown Peaks **************************
Pk. Num
Ret Component Time Name
Concentrt ion
Height
A r e a B1 . D e l t a Cede
1 1.60 2 1.88 3 2.25 4 3.02 5 8.03 6 12.62 7 13.00 8 13.40 9 15.60 10 17.48
0.000 0.000 0.000 0.000 0 .OCO 0.000 0.000 0.000 0.000 0.000
69488 21340 16588 67032
5580 21924 20808 19219 18582
4081
750126 203220 109247 5254413 252489 309643 281658 252303 320398
48810
1 1 1 1 1 1 2 2 1 1
Totals
0.000
264643
7782307
Schedule: \peaknet\schedule\afffll_2.sch Release 4.30
B-41
. 11/3/97 1:21:11 PM
US00001047
File: afff0035.DXD Sample A2 end o f settle--control
100 ; i
80 i
60 I
40 i
nC 20
0 -20
:23 4
9 10
5 678 y "
-40 '
,
0 2 4 6 8 10 12 14 16 18 Minutes
20
Schedule: \peaknet\schedule\afffll_2,.sch
Release 4.30
'
B-42
11/3/97 1:21:12 PM US00001048
Data File : c : \ p e a k net\data\afff0036.DXD Sample Name: A3 end of settle--control
Inject #
: 36
Method File: c :\peaknet\method\afff.met
System Name: DX-500
Column Type :
Data Points: 1200
Module Name:
Report Date Collected Vial # Calibrated Detector Operator Rate Moduleware
11/3/97 1:49:41 PM 11/3/97 1:21:19 PM
11/2/97 10:04:00 PM ED4 0
1.00 Hz 1.17
ti II II II II II II II II II II II II II If II II II II II Il M Il II Il K) II II II O II II II CD il in
Calibracin Volume
Dilution Start
Scop Area Reject P k . Width Threshold
External
1
1 0.00 19.98
1000
10.00
19.21
it***************** C o m p o n e n t R e p o r t : A l l C o m p o n e n t s * * * * * * * * * * * * * * * * * * * * * * *
Pk.
Ret Component
Num Time Name
Concentration
0 0.00 butylcarb
0.000
Totals
0.000
Height
0 0
Area B l . %Delta Code
0 0 0.00
0
************************* Peak Report: Unknown Peaks it****************
Pk. Num
Ret Component Time Name
1 1.68 2 1.87 3 7.32 4 3.08 5 S .20 6 12.45 7 12.85 8 13.25 9 13.65 10 15.08 11 16.57 12 1 7 . 5 5
Concentration
Totals
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
0.000
Height
96669 37743 18237 48865
5214 15489 24772 20861 11321 49341 19091
4535
352139 *
A r e a B.l. Code
762671 347577 164863 3864357 306663 215466 331377 273990
78122 6169717
367986 61938
1 1 .1 1 1 1 2 2 1 1 1 1
12944726
S c h e d u l e : \ p e a k n e t \ s c h e d u l e \ a f f f 11 2.sch
Release 4.30
"
B-43
11/3/97 1:49:41 PM
US00001049
File: afff0036.DXD Sample A3 end of settle-control 100 I,
0O0 oCN
60 | 40 j
0; -20
-40
1!!2? 3 4
A'
10 11 12 6789 -
IIl 8 10 12 14 16 18 20
Minutes
Schedule: \peaknet\schedule\afff11_2.sch
Release 4.30
"
B-44
11/3/97 1:49:41 PM
i
US00001050
Data File : c :\peaknet\data\afff0037.DXD
Sample Name: Bl end of settle--60ppm
Inject #
: 37
.
Method File: c :\peaknet\method\afff.met
System Name: DX-500
Column Type:
Data Points: 1200 Module Name:
I D : 24 0a 85
Report Date Collected Vial # Calibrated Detector Operator Rate ' . Moduleware
11/3/97 2:18:09 PM 11/3/97 1:49:48 PM
11/2/97 10:04:00 PM ED4 0
1.00 Hz 1.17
C a l i b r a t i o n V o l u m e D i l u t i o n S t a r t S t o p A r e a R e j e c t Pk. W i d t h T h r e s h o l d
External
1
1 0.00 19.98
1000
10.00
19.21
********************** Component Report: All Components ***********************
Pk. Num
0
Ret Component Time Name
0.00 butylcarb
Concentration .
0.000
Height 0
Area B l . %Delta ' Code
00
0.00
Totals
0.000
00
*********** ********** **** peak Report Unknown Peaks **************************
Pk . Num
Ret Component Time Name
Concentrt ion
1 1.67 2 1.87 3 2.33 4 3.12 5 8.28 6 11.25 7 12.28 8 12.60 9 12.98 10 13.38 11 1 3 . 8 0 12 17.10
0.000 0.000 0.000 0.000 - 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Totals
0.000
Height
731756 45162 24952 64692 4061 5036 29980 16870 31510 11383 30956 6116
1002473 *
Area B l . Code
3594938 549883 190974
1132008 43338 30743
742390 169948 439257
86009 910981
91389
3 4 1 1 1 1 1 1 1 1 1 1
7981857
S c h e d u l e : \ p e a k n e t \ s c h e d u l e \ a f f f 1 1 _ 2 .s c h Release 4.30
B-45
11/3/97 2:18:09 PM
US00001051
,File: afff0037.DXD Sample B1 end of settle-60ppm
100 I
80 i
60 !
40 I
nC 20 ;
0
'2 3
11 78910;
12
-20
-40
I:!I
10 12 14 16 18
Minutes
20
Schedule : \peaknet\schedule\afff11_2.sch
Release 4.30
~
B-46
11/3/97 2:18:09 PM
1
US00001052
Data File Sample Name Inject # Method File System Name Column Type Data Points Module Name
c :\peaknet\data\afff 0 0 3 8 .DXD B2 end of s e ttle--60ppm 38 c :\peaknet\method\afff.met DX-500
1200
Report Date Collected Vial # Calibrated Detector Operator Rate Moduleware
11/3/97 2:46:37 PM 11/3/97 2:18:17 PM
11/2/97 10:04:00 PM ED4 0
1.00 1.17
Hz
.
in ii CO II
ii n 5 11 O II
II
^ II
( N II
Q II M II
II 11 II II II II II II II II II II II II II II II II II II
Calibration Volume Dilution Start Stop Area Reject P k . Width Threshold
External
1
1 0.00
19.98
1000
10.00
19.21
********************** Component Report: All Components ***********************
Pk. Num
0
Ret Component
Time Name
.
Concentration
0.00 butylcarb
0.000
Totals
0.000
Height
0 0
Area B l . %Delta Code
0.0
0.00
0
* ******* ***************** pg ^ R e p o r t ' U n k n o w n Psks * * * * * * * * * * * * * * * * * * * * * * * * * *
Pk . Num
Ret Component Time Name
Concentration
1 1.67 2 2.35 3 3.02 4 8.30 5 11.47 6 12.18 7 12.58 8 12.98 9 16.33 10 17.20
.
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Totals
0.000
Height .
Area B l . Code
807134 25120 18797 5800 46886 42077 42530 32363 6258 5398
3788001 170123 279215 306504
4236383 695237 597959 472836 98575 81695
1 1 1 1 2 2 2 2 1 1
1032364
10726528
S c h e d u l e : \ p e a k n e t \ s c h e d u l e \ a f f f 1 1 _ 2 .s c h
Release 4.30
',
B-47
11/3/97 2:46:37 PM
US00001053
File: afff0038.DXD Sample B2 end of settle--60ppm
100 ,
i
80 ;
60 i
40 ;
nC 20 ! 0-
-20 :
lM! 2 o^
5 678
9 10
I:
-40
ii 6
' !M ' I IIj
10 12 14
16
i
18
Minutes
20
Schedule: \ p e a k n e t \ s c h e d u l e \ a f ff11 2.sch Release 4.30
B-48
11/3/97 2:46:37 PM US00001054
Data File Sample Name Inject # Method File System Name Column Type Data Points Module Name
c :\ p e a k n e t \ d a t a \ a f f f 0 0 3 9 . D X D B3 end of settle--60ppm 39 c :\peaknet\method\afff.met DX-500
1200
Report Date Collected Vial # Calibrated Detector Operator Rate Moduleware
11/3/97 3:15:06 PM 11/3/97 2:46:45 PM
1 1 / 2 / 9 7 10 :0 4 :00 P M ED4 0
1.00 Hz 1.17
IT) II CO II
II fO II O II
II II ( N II
II
ah
M II II II II II II II II II II II II II II II II II II It II II
Calibration Volume Dilution Start Stop Area Reject P k . Width Threshold
External
1
1 0.00
19.98
1000
10.00
19.21
********************** Component Report: All Components ***********************
Pk. Nun
0
Ret Component Time Name
Concentration .
0.00 butylcarb
0.000
Totals
0.000
Height
0 0
Area B 1 . %Delta Code
0 0 0.00
0
* * * * * * * * * * * * * * * * * * * * * * * * P s k R e p o r t : U n k n o w n P e a k s ** * * * * * * * * * * * ** *********,
Pk. Nun
Ret Component Time Name
Concentration
Height
Area Bl. %Delta Code
1 1.68 2 2.40
3 2.93 4 3.23 5 11.32 6 11.98 7 12.45 8 12.85 9 13.73 10 17.35
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
878640 22488 44389 44063 21378 14029 34516 27980 30548 4947
4085513 144821 642685 579254
2181840 , 95452
473184 369718 1457236
80618
1 1 2 2
l 2 2 1 1
.
Totals
0.000
1122978
10110321
S c h e d u l e : \ p e a k n e t \ s c h e d u l e \ a f f f l l _ 2 .s h
Release 4.30
"
B-49
11/3/97 3:15:06 PM
US00001055
File: afff0039.DXD Sample B3 end of settle-60ppm
100 1
80
60
40
nC 20 i
0L
2 ?4
10
-20 i
-40
0 2 4 6 8 10 12 14 16 18 Minutes
20
Schedule : \peaknet\schedule\afff11_2.sch Release 4.30
B-50
11/3/97 3:15:06 PM US00001056
Data File : c:\peaknet\data\afff0040.DXD
Sample Name: STANDARD 4-S0ppm
Inject #
: 40
M e t h o d File: c : \ p e a k n e t \ m e t h o d \ a f f f .met
System Name: DX-500
Column Type:
Data Points: 1200
Module Name:
I D :24 0a 85
Report Date Collected Vial # Calibrated Detector Operator Rate Moduleware
11/3/97 3:43:34 PM 11/3/97 3:15:14 PM
1 1 / 2 / 9 7 10 :04 :00 P M ED4 0
1.00 Hz 1.17
Calibration Volume
Dilution Start
Stop Area Reject P k . Width Threshold
External
1
1 0.00 19.98
1000
10.00
19.21
*********** *********** Component Report: All Components **** *******************
Pk. Num
Ret Component Time Name
Concentration
Height
Area Bl . %Delta Code
0 0.00 butylcarb
0.000 0 0 0 0.00
Totals
0.000
0
0
* ** ********************** Peak Report: Unknown Peaks **** ********* *************
Pk. Num
Ret Component Time Name
Concentration
Height
Area 31. %De l t a Code
1 2 3 4 5 6 7 8 .9 10 11 12 13 14
1.78 2.20 2.45 2.98 3.27 3.60 3.92 10.85 11.33 12.12 12.52 12.92 13.32 13.52
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
325296 24002 18971 7210
209860 111458 101553
5067 10457 24539 14869 22302 133338 84206
2429357 170707 148875 50711
2375017 1482906 1071935
65565 466714 337970 125229 278019 609577 1622195
2 2 2 2 2 2 2 1 1 1 1 1 2 2
Totals
0.000
1093126
11234776
S c h e d u l e : \ p e a k n e t \ s c h e d u l e \ a f f f 1 1 _ 2 .s c h
Release 4.30
.
B-51
11/3/97 3:43:34 PM
US00001057
File: afff0040.DXD Sample STANDARD 4-50ppm 100
80
60
40 ! nC 20 i
0-20 :
!
I67
! 23 4:U I
YV A
8 9 101112
l i b i l i 7'
-40
0 2 4 6 8 10 2 14 16 18 20
Minutes
Schedule: \peaknet\schedule\afffli 2 .sch
Release 4.30 B-52
11/3/97 3:43:34 PM US00001058
BNR Inhibition Batch Assay Pretreated with Defoamer 8710 (A FFF 60 ppm)
B-53
US00001059
Reactor A1 A2 A3 B1 B2 B3
AFFF (ppm )
0 0 0
60
60 60
D efoam er 8710 m l/lher
Stage
F e e d s to c k
R R Decant 0 End of Feeding
End of Anaerobic E nd of A erobic E n d of Settling
0 End of Feeding End of Anaerobic End of A erobic
End of Settling 0 E n d of Feeding
End of A naerobic
E n d of A erobic
E n d of Settling 7 5 E nd of Feeding
End of Anaerobic E nd of A erobic End of Settling
7 5 End of Feeding End of Anaerobic End c f Aerobic E n d of Settling
7 5 E n d of Feeding End of Anaerobic
End of Aerobic E n d of Settling
Tim e
2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr 8 hr
NH 3-N (m g /L )
TKN (m g/L)
30.50 1 49 .29
1.58 2 1 .2 5
1 .5 6 2 6 .5 5
2 3 .4 6 6 .5 9 5 .8 5
3 1 .0 9 1 5 .2 4
6 .3 4
2 1 .2 5 22.56
6 .3 4
2 4 .2 8 2 9 .6 5 1 3 .9 0
6 .3 2 19.62 2 1 .6 8
6 .3 4 3 3 .2 2 2 6 .9 6
6 .1 0
1 1 .4 5
5 .2 0 17.41
7 .3 1 4 7 .5 4
2 0 .0 3 21.40 2 2 .9 9
3 2 .6 0 2 6 .9 6 2 9 .1 0
1 6 .7 3 2 0 .0 3 2 0 .5 8 22.11 16.73 1 9 .2 6
3 9 .7 4 3 5 .8 6 2 6 .9 6 2 6 .4 5 4 3 .4 6 3 7 .6 0
2 0 .5 8 22.11
2 2 .2 9 3 0 .5 1
File: AFFF Inhib (28-9-97).xls Sheet: Standards & Data
B-54
US00001060
erIC Colum n S tandard Curve
m - 4 .0 0 E -0 8
/= 1 .6 2 92 0 5 88
0OOE+ 1 OOE+ 2 .0 0 E + 3 0 0 E + 4 .0 0 E + 5 .0 0 E + 6 .0 0 E + 7 .0 0 E + 8 0 0 E +
00 0 9 0 9 0 9 0 9 0 9 0 9 0 9 0 9
C f C o n e, m gfl
IC Column MOZ - N Standard Curve
m - Z O O E -08 / = -2 .4 4 E -0 1
m = ZO O E -08
i- 6 .5 8E -01
IC Column P O /- P Standard Curve
m= S.OOE-08
i = 8 .8 0 E -01
O.OOE+ 1 .0 0 E + 2 . 0 0 E + 3 . 0 0 E + 4 . 0 0 E + 5 . 0 0 E + 6 . 0 0 E + 7 . 0 0 E + 8 . 0 0 E +
000808080808080808
P O i- P C o n e, m g /l
File: AFFF Inhib (28-9-97).xls Sheet: Standards & Data
B-55
US00001061
A FFF R e a cto r (ppm )
A1 0
D efoam er 8710
m l/liter
Stage
Feedstock
R R Decant 0 End of Feeding
C l-
Tim e
A re a
(m g/L)
7457747666 282.4
5623726490 2 hr 5041945872
2 1 3 .4 191.5
N O j'-N
NCV-N
PO/-P
A re a
(m g/L)
A re a
(m g/L)
A re a
(m g/L)
0 0.0 3524379 0.7 507017629 27.8
0
0.0 1706044005 30.1
10153030
1.4
0 0.0 2758118 0.7
-
-
A2 0 A3 0
End of A naerobic 4 hr 5027455061 190.9
0
0.0 2964817 0.7 944779425 51.0
End of A erobic
6 hr 5053058153 191.9 85235354
1.8 1291777953 22.9 562010370 30.7
End of Settling
8 hr 5088956393 193.2 78609565
1.6 1341478644 23.8 552997441 30.2
0
End of Feeding
2 hr 5 1 2 7 4 00 4 1 2 194.7
0
0.0 1329484 0.7
-
-
End of A naerobic End of Aerobic
4 hr 4946719672 6 hr 5003826950
187.9 190.0
0 75976408
0.0 817360 0.7 958696272 51.8 1.5 1278850617 22.7 493873713 27.1
End of Settling
8 hr 5117089552 194.3 73973601
1.5 1315150459 23.3 495640650 27.2
0
End of Feeding
2 hr 5091896779 193.3
0
0.0 226258 0.7 913093284 4 9 3
End of A naerobic 4 hr 4940329563 187.6
0
0.0 1800473 0.7 1002070135 54.1
B1 60 B2 60 B3 60
End of Aerobic
6 hr 4975297553 188.9 91086799 1 .9 * 1255680352 22.3 545344963 29.8
End of Settling
8 hr 5056091891 192.0 81130861
1.7 141614B773 25 1 547369867 29.9
75
End of Feeding
2 hr 4977058840 189.0
0
0 .0 111277732 2 .6 9 0 7 9 5 4 3 1 2 49.1
End of A naerobic 4 hr 4737157675 180.0
0
0 0 4703645 0.7 1033593354 55.7
End of Aerobic End of Settling
6 hr 4857096710 184.5 72317154 1.4 102657989 2.4 760675975 41.3 8 hr 509630B355 193.5 83028403 1.7 134183815 3.0 767856471 41 .6
75
End of Feeding
2 hr 4 7 8 3 9 3 0 8 8 3 181.7
0
0.0 30003358 1.2 922896041 49.9
E n d of A naerobic 4 hr 4825396022 183.3
0
0.0 2363790 0.7 1051995712 56.7
End of Aerobic
6 hr 4 9 2 7 3 2 2 6 0 4 187.1 50 2 5 9 48 7 0.9
185767797
3.9 752829263 40.8
End of Settling
8 hr 5028328616 190 9 52302672 1 0 202345313 4 1 754358389 40.9
75
End of Feeding
2 hr 5 0 7 9 1 8 0 1 0 4 192 9
0
0 .0 870556 0.7 9 6 4 4 9 12 6 5 52.1
E n d of A n a e ro b ic 4 hr 4 9 8 9 2 6 4 2 3 0 189 5
0
0.0 2805377 0.7 1119659940 60.3
End of Aerobic End of Settling
Stand 2
6 hr 51 8 0 7 72 5 3 6 8 hr 5 4 1 4 7 07 8 0 6
2537327961
196.7 63924582 205 58302078 97.2 436373497
13 1.12 100
122078432 147594599 498937800
2.8 853373379 46.2 3.2 832463719 45.1 9.3 157251445 9.2
Stand 3 Stand 4 Standards used
5201369190 8233404885
197.5 3 1 1 .6
877244997 1 .7 7 2 E + 0 9
2 0 .3 4 1 .2
1107112362 2506862853
19.8 43.9
337460091 827751035
18.8 44.8
STD i STD 2 STD 3
112034
0
00
0
0
0
0
2546706893 100 437097596 10 499196654
10 159790402 10
5273486188 200 886725171 20 1111316466 20 346916597 20
STD 4
7943185575 300 1.707E+09 40 2294176939 40 745786620 40 .
Phosphorus results derived from 1C.
"V alues not known
File: A FFF Inhib (28-9-97).xls Sheet: Standards & Data
B-56
!
I
US00001062
SUMMARY OUTPUT
CL
R eg ression Statistics
R Square O bservations
0 .9 9 9 8 1 2 7 6 3 4
C oefficients S tan d ard E rro r
In te rc e p t X Variable 1
1 .6 2 9 2 0 5 8 7 7 3.7S 49 2E -08
1 .7 9 7 6 4 2 7 1 1 3.64315 E -10
SUMMARY OUTPUT
N 0 2 -N
R egression Statistics
R Square O bservations
0 .9 9 9 5 3 9 3 9 4
In te rc e p t X Variable 1
C oefficients S tan d ard E rro r
-0.2 441163 98
0 .3 5 0 6 0 6 5 4
2.34176E -08
3.55 4 6 3 E -1 0
SUMMARY OUTPUT
NO >N
R egression Statistics
R Square
0 .9 9 8 7 9 9 2 7 3
O bservations
4
Intercept X Variable 1
C oefficients S tan d ard E rro r
0 .6 5 7 5 5 5 5 3 1
0 .5 4 9 3 9 7 9 3 7
1.72536 E -08
4230G 6E -10
SUMMARY OUTPUT
P 0 4 -P
R eg ression Statistics
R Square
0 .9 9 7 8 3 1 6 4 6
O bservations
4
In te rc e p t X Variable 1
C oefficients S tan d ard E rro r
0 .8 7 9 6 6 7 4 8 5
0 .7 3 3 0 0 9 2 8 4
5.30 7 9 2 E -0 8
1.74963E -09
File: AFFF Inhib (28-9-97).xls Sheet: Standards & Data
B-57
US00001063
Reactor
AFFF (ppm )
A1 0
A2 0
A3 0
B1 6 0 B2 60 B3 60
Defoam er 8710 m l/liter 0 0 0
75 75 75
Stage F e e d s to c k RR Decant E n d of Feeding End of Anaerobic E n d of Aerobic E n d of Settling E n d of Feeding E n d of Anaerobic E n d of Aerobic E n d of Settling E n d of Feeding E n d of Anaerobic E n d of Aerobic E n d of Settling E n d of Feeding E n d of Anaerobic E n d of Aerobic E n d of Settling E n d cf Feeding E n d of Anaerobic E n d o f Aerobic E n d of Settling E n d of Feeding E n d of Anaerobic E n d erf A e ro b ic E n d o f Settling
STD 1 STD 2 STD 3 STD 4 STD 5 F S (Filtered) FS A verage R R S U (Filtered) R R S U Average
Tim e
2 hr 4 hr 6 hr 8 hr 2hr 4 hr 6 hr 8hr 2 hr 4hr 6 hr 8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr 8 hr
COD
ABS (mg/L)
% COD Removal
0.091 1189.7
0.001 0 .0 1 2
5 .9 1 5 0 .6
6 2 .4
0 .0 1 5 190.0
0.001
5 .9
0 .0 0 3
3 2 .2
0 .012 150.6
0 .015 190.0
0 .0 0 6
7 1 .6
0 .0 0 5
5 8 .5
0 .0 1 0 124 .3
0 .012 150 .6
0.001
5 .9
0 .0 0 3
3 2 .2
0 .0 5 3 689.9
0 .0 5 2 676.7
0 .070 913.5
0 .0 7 1 0 .0 5 8
9 2 6 .7 7 5 5 .7
0 .0 5 3 689.9
0 .0 7 2 9 39 .8
0.070 913.5
0 .0 5 5 716.2
0 .0 5 6 729.3
0 .077 1005.6
0 .0 7 5
9 7 9 .3
0 .0 0 0
0
0 .0 7 7
1000
0 .1 4 9
2000
5 2 .6 9 8 .5 9 2 .0 6 2 .4 5 2 .6 821 8 5 .4 69 .0 6 2 .4 9 8 .5 9 2 .0 6 1 .7 6 2 .4 4 9 .3 4 8 .6 5 8 .0 6 1 .7 4 7 .8 4 9 .3 6 0 .2 5 9 .5 4 4 .2 4 5 .6
0 .2 3 6
3000
0 .3 3 9 0.091 0.091 0.001
4500 1 1 8 9 .7 1 1 8 9 .7
5 .9
0 .0 0 1
5 .9
* The values of "COD % Removal" shown in table and chart above are accumulative figures based on the initial COD concentration at time 0 hr.
File: A FFF Inhib (28-9-97).xls Sheet: COD
B-58
US00001064
Initial COD at Time 0 hr.
S am p le C o n tro ls (A 1A 2S A 3)
C o n s titu e n t R R Decant F e e d s to c k Defoam er
Vol (L) 4 2 2
C O D m g/L 5 .9
1 1 8 9 .7 0
2 3 .4 7 9 6 9 8 1 2 3 7 9.47 486
0
Inhij'rtion (B 1.B 2& B 3)
Reactor (AFFF ppm ) % COO Removal
A1 (0) 9 2 .0
AFFF Total R R Decant Feedstock D efoam er AFFF T o ta l
A 2 (0 ) 8 5 .4
0
6 4
2 1
2
6 A 3 (0)
9 2 .0
o
5 .9 1 1 8 9 .7
4930 1737
B1 (10) 4 8 .6
400 .5
23 .6 237 9 .4
4930 3474 18 0 1 .2 B 2 (10) 4 9 .3
% COD Removal
B 3 (10) 4 5 .6
100.0
9 0 .0 80.0 - 7 0 .0 6 0 .0
aK 5 0 .0 - 40 0 30.0 - 2 0 0 10.0 0.0
9 2 .0 A1 (0)
8 5 .4 A 2 (0)
9 2.0 4 8.6
A 3 (0)
B 1 (10 )
R eactor (A FFF ppm )
B2 (10)
B3 (10)
COD Standard Curve
4UU * 4000 3500 =! 3000 E 2500 O 2000
8 1500
1000 500 0< 0 .000
y = 1 3 1 5 4 X -7 .2 8 4 2 Ft2 = 0 . 9 9 6 8
0 .050
0 .1 0 0
0 .1 5 0
0 .2 0 0
0 .2 5 0
A B S O R B A N C E U N IT S (F O R 600 n m )
0 .3 0 0
0 .3 5 0
m ~ 13154
/ = -7.2842
File: A FFF Inhib (2&-9-97).xls Sheet: COD
B-59
US00001065
SUMMARY OUTPUT
COD
R egression Statistics
R Square O bservations
0.99884 7775 5
C oefficients
Intercept X V ariable 1
-7.2 8 4 1 5 8 8 7 1 1 3 1 5 4 .0 8 3 3 9
S tandard E rro r 5 1 -4 2 6 1 8 4 4 3 2 5 7 .940 1893
File: A FFF Inhib (28-9-97).xls Sheet: COD
B-60
US00001066
6.00
5.00 -
ra= ) 4 .0 0 .
E 3.00 -
2.00 1 .0 0
0 .0 0
D issolved O xygen
. `
b 29%***< -
F e e d in g
A n a e ro b ic STAGE
A erobic
Oxygen Uptake during Aerobic Stage
9A 1 BA2 A 3 B1 B2
File: AFFF Inhib (28-9-97).xls Sheet: Oxygen Uptake
B-61
US00001067
S ta g e F e e d in g A n a e ro b ic A e ro b ic
Dissolved Oxygen (mg/L) at various stages
A1 A 2 A 3 B1 B 2
0 .2 0
0 .1 7
0 .1 3
0 .1 3
0 .0 9
0 .0 7
0 .1 3
0 .0 2
0 .0 7
0 .0 8
4 .3 8
4 .4 0
5 .0 2
0.41
0 .4 3
B3 0 .0 7 0 .0 3 0 .5 3
S ta g e A erobic
Tim e (sec) 0
20 40 60 80 100 120 140 170 200 230 260 290 320 350 380 410 440 470 500 530 560 590 620 650 680 710 740
A1 7 .8 0 7.04 6.81 6 .7 4 6 .6 7 6 .6 2 6 .5 6 6 .4 9 6 .4 0 6 .3 2 6.24 6 .1 7 6.09 6 .0 2 5 .9 6 5 89 5.83 5.76 5 .7 0 5.64 5.58 5.53 5 .4 7 5.41 5 .3 6 5.31 5 .2 5 5 .2 0
Dissohred O xygen in m g/L
A2 A3
B1
7 .8 5
7 .8 4
798
7 .0 5
7 .4 6
7.31
6 .8 4
7 .3 0
7.11
6 .7 3
7 .2 3
7.01
6 .6 6
7 .1 6
6 .9 3
6 .6 0
7.11
6 .8 6
6 .5 4
7.04
6 .7 9
6 .4 8
6 .9 8
6 .7 3
6 .4 0
6.91
6 .6 4
6 .3 2
6 .8 3
6 .5 6
6 .2 5
6 .7 5
6 .4 9
6 .1 8 6.11 6 .0 5 5 .9 8
6 .6 9 6 .6 2 6 .5 6 6 .4 9
6.41 634 6 .2 7 6 .2 0
5 .9 2 5 .8 9
6 .4 3 6 .3 7
6 .1 3 6 .0 7
5 .8 0 5.74
632 6 .2 6
6.01 5 .9 4
5 .6 8 5 .6 3
6 .2 0 6 .1 4
5 .8 9 5 .8 3
5 .5 8
6 .0 9
5 .7 7
5 .5 2 5 .4 7
6 .0 4 5 .9 9
5 .7 2 5 .6 7
5.42 5 .3 7
5 .9 3 5 .8 8
5 .6 2 5 .5 6
5 .3 2 5 .2 7
5 .8 3 5 .7 8
5.51 5 .4 6
B2 7 .8 5 7 .3 7 7 .2 0 7 .1 2 7 06 7.01 6 .9 5 6 .89 6.81 6 .7 4 6 .6 6 6 .5 9 6 .5 3 6 .4 6 6 .4 0 634 6 .2 7 621 6 .1 6 6 .1 0 6 .0 4 5 .9 9 5 .9 4 5 .8 8 5 .8 3 5 .7 8 5 .7 3 5 .6 8
B3 8 .0 6 7 .4 0 7 .1 9 7 .1 0 7 .0 3 6 .9 6 6 .9 0 6 .8 3 6 .7 5 6 .6 7 6 .5 9 6.51 6 .4 4 6 .3 7 6 .3 0 6 .2 4 6 .1 7 6.11 605 5 .9 9 5 .9 3 5 .8 8 5 .8 2 5 .7 7 5.71 5 .6 6 5.61 5 .5 6
File: A FFF Inhib (28-9-97).xls Sheet: Dissolved Oxygen
B-62
US00001068
Reactor A1 A2 A3 B1 B2 B3
AFFF (ppm )
0
0 0
60 60 GO
D efo am er 8710 m l/1iter
Stage F e e d s to c k
R R Decant
0 E n d of Feeding E nd of Anaerobic
E n d of Aerobic E n d of Settling
0 E n d of Feeding E n d of Anaerobic
E n d erf A e ro b ic E n d of Settling
0 E n d of Feeding E nd of Anaerobic
End of A erobe
E n d of Settling
7 5 E n d of Feeding E n d erf A n a e ro b ic
E n d erf A e ro b ic E n d of Settling 7 5 E n d of Feeding E n d of A naerobic E n d of Aerobic E n d of Settling
75 E n d of Feeding End of Anaerobic E n d of Aerobic E n d of Settling
T im e
2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr 8 hr
C o n c e n t r a t io n , m g IL
BOD COD
TOC
1189.7 4 2 0 .0
5 .9 10.8
1 5 0 .6
1 9 .9
1 9 0 .0
15.1
5.9 9.7
3 2 .2
9 .5
1 5 0 .6
2 0 .0
1 9 0 .0
14.7
7 1 .6
8 .9
5 8 .5
9 .0
1 2 4 .3
2 0 .6
1 5 0 .6
14.9
5.9 8.8
3 2 .2
9 .0
6 8 9 .9 149.8
6 76 .7 147.7
9 1 3 .5 164.7
9 2 6 .7 171.1
7 5 5 .7 145.8
6 8 9 .9 148.0
9 3 9 .8 167.1
9 1 3 .5 170.1
7 16 .2 151.7
7 2 9 .3 146.3
1005.6 173.0
9 7 9 .3 173.3
Alkalinity H C 0 3 <mg/I)
7 71 .0 3 3 3 .0 3 7 0 .0 3 84 .0 2 07 .0 2 16 .0 3 6 8 .0 3 6 3 .0 2 2 7 .0 2 1 4 .0 3 68 .0 3 8 6 .0 2 19 .0 2 13 .0 3 6 5 .0 3 5 1 .0 3 5 0 .0 2 94 .0 3 6 7 .0 3 3 3 .0 3 44 .0 3 2 7 .0 3 64 .0 3 4 0 .0 3 4 6 .0 3 30 .0
TOC -vs.- COD
File: A FFF Inhib (28-9-97).xls Sheet: Concentrations Table
B-63
US00001069
Date of Test: 3-11-97
NH3-N & TKN Charts
AFFF Nitrification Inhibition in terms of NH3-N
-- -- A1 - X -- A2
A3 -- -B 1 -----------B 2 -- B3
Inhibition Effect of AFFF on Nitrification in terms of N03-N
T im e (hrs)
Inhibition Effect of AFFF on Nitrification in terms of N02-N
A1 ,A2,A3-- Control Reactors B1 ,B2,B3-- Inhibition reactors->60ppm A FFF with Defoamer 8710
File: AFFF Inhib (28-9-97).xls Sheet: NH3-N & TKN Charts
B-64
<
US00001070
Date of Test: 3-11-97
NH3-N & TKN Charts
Inhibition Effect of AFFF on Nitrification in terms of TKN
A1 ,A2,A3-- Control Reactors B1 ,B2,B3-- Inhibition reactors->60ppm AFFF with Defoamer 8710
Rie: A F F F Inhib (28-9-97).xls Sheet: NH3-N & TKN Charts
B-65
US00001071
D ate of Test: 3-11-97
Inhibition Reactors / Nitrogen M ass Balance
Reactor A1 (No AFFF Present)
N02-N N03-N BOrg. N 0NH3-N
STAGE
Reactor A2 (No AFFF Present)
iPililff: lltf
End of F e e d in g
End of A n aero b ic
End of A erobic
STAGE
0N 02-N D N 0 3 -N BOrg. N EJNH3-N
E n d of S e ttlin g
Reactor A3 (No AFFF Present)
D N 02-N D N 0 3 -N BOrg. N NH3-N
STAGE
File: AFFF Inhib (28-9-97).xls Sheet: Nitrogen Mass Balance
B-66
US00001072
D ate of Test: 3-11-97
Inhibition Reactors / Nitrogen Mass Balance
Reactor B1 (60 ppm AFFF with Defoamer 8710)
60 ----------
J 50
D N 0 2 -N
E 40
go>3 0
2 20 z 10
0
End of F e e d in g
fef III
sUsMMpiHUlil'
End of A n a e ro b ic
End of A erobic
STAGE
lIliIfiPe
m
End of S e itfin g
N 0 3 -N B O rg .N 3 N H 3-N
STAGE
45 40
=! 35
I* 30
coo>25
o
r
V- 5r:'
End of F e e d in g
|Ste:
End of A n a e ro b ic
End rf A e ro b ic
STAGE
...
End of S e ttlin g
N 0 2 -N N 0 3 -N B O rg. N B N H 3-N
File: A F F F Inhib (28-9-97).xls Sheet: Nitrogen Mass Balance
B-67
US00001073
A1 (ControO
0
A2 (ControO
0
A3 (ControO
0
B1 6 0
B2 60
B3 . 60
E 0 Q
U. u. u. <
Defoam e r 8710 m l/lite r 0
0
0
75
75
75
S tag e E n d of Feeding
E n d o f Anaerctoic E n d o f Aerctoic E n d of Settling E nd of Feeding
E n d o f Anaerctoic E n d of A erobic E n d of Settling End of Feeding
E n d of A naerobic E n d o f Aerctoic E n d of Settling E n d of Feeding
End of A naerobic E nd of A erobic E n d of Settling E n d of Feeding
End of A naerobic E n d of A erobic E n d of Settling E nd of Feeding
End of A naerobic E n d of A erobic E n d of Settling
Tim e 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr
6 hr 8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr 8 hr
N itrogen C o n cen tratio n , mg/L
N H 3-N N 0 3 -N
N 0 2 -N
A (N O ,-N )
A (N O rN )
2 1 .25
0 .7
0 .0
2 3 .4 6
0 .7
0 .0
6.5 9 5 .8 5
2 2.9 2 3.8
1 .8 1 .6
2 3 .1 0
1 .6 0
2 1 .25
0 .7
0 .0
2 2 .5 6
0 .7
0 .0
6 .3 4 6.3 2
2 2 .7 2 3.3
1 .5 1 .5
2 2 .6 7
1 .4 9
1 9 .6 2
0 .7
0 .0
2 1 .6 8
0 .7
0 .0
6 .10
2 2 .3
1 .9
5 .2 0
25.1
1 .7
2 4 .4 3
1.66
17.41
2 .6
0 .0
2 0 .03
0 .7
0 .0
2 1 .4 0 2 2 .9 9
2 .4 3 .0
1 .4 1 .7
0 .4 0
1.70
1 6 .7 3
1 .2
0 .0
2 0 .0 3
0 .7
0 .0
2 0 .5 8 22.11
3 .9 41
0 .9 1 .0
2 .9 7
0 .9 8
16.73
0 .7
0 .0
1 9 .2 6
0 .7
0 .0
2 0 .5 8 22.11
2 .8 3 .2
1 .3 1.1
2 .5 3
1.12
30.00 i
25.00 -
-I
20.00 E
z 15.00 Xz 10.00
5 .00-
5.85
is
A1
Ammonia Remaining (Supernatent)
&32
,m
A2
A3
File: AFFF Inhib (28-9-97).x!s Sheet: N 0 3 - & N 0 2 Produced
. B-68
US00001074
Nitrite (supernatant)
f
File: A FFF Inhib (28-9-97).xls Sheet: N 0 3 - & N 0 2 Produced
B-69
US00001075
BNR Inhibition Tests - 60 ppm AFFF Pretreated with Defoamer 8710 (Sept/28/97)
TSS Reactor A1 A2 A3 B1 B2 B3
Initial wt Final w t Volume MLSS
1.0961 1.1459
15 3320
1.1036 1.1538
15 3347
1.1044 1.1533
15 3260
1.1089 1.1674
15 3900
1.1127 1.1712
15 3900
1.1113
1.167
15 3713
W T @ 55 MLVSS 1.1002 3047 1.1084 3027 1.1092 2940 1.1165 3393 1.1202 3400 1.1185 3233
TDS Reactor A1 A2 A3 B1 B2 B3
Initial wt Final wt Volume TDS
1.025 1.0399
15 993
1.0187 1.0328
15 940
1.0177
~
15
1.0219
1.038
15 1073
1.0168 1.0348
15 1200
1.0177 1.0359
15 1213
TS Reactor A1 A2 A3 B1 B2 B3
Initial wt Final w t Volume TS
1.0258 1.0913
15 4367
1.0094 1.0732
15 4253
1.0229 1.0825
15 3973
1.0231
1.099
15 5060
1.0271 1.1051
15 5200
1.0198 1.1007
15 5393
ZTSS,TDS 4313 4287
-
4973 5100 4927
File: AFFF Soilds Data
B-70
US00001076
BNR Inhibition Batch Assay Pretreated with Defoam er AF9020 (AFFF 60 ppm)
B-71
US00001077
R e a c to r A1 A2 A3 B1 B2 B3
AFFF (ppm )
0 0 0
60
60 60
D efoam er A F 9020 m tffite r
Stage
Feedstock R R Decant 0 End of Feeding
End of Anaerobic End of A erobic E n d o f Settling 0 E n d o f Feeding End of Anaerobic E n d of Aerobic
E n d of Settling 0 End o f Feeding
End of Anaerobic End of A erobic E n d of Settling 15 End of Feeding
End of Anaerobic End of Aerobic E n d of Settling 15 End of Feeding End of Anaerobic End of A erobic End of Settling 15 End of Feeding E nd of Anaerobic End of Aerobic End of Settling
N H 3-N
Tim e (mgrt.)
2 6 .7
2 hr
0.1 2 4 .6
4 hr
3 0 .0
6 hr 8 hr
4 .7 2 .8
2 hr 4 hr 6 hr
2 4.0 2 9 .4
4 .5
8 hr 2 hr
3 .2 2 5 .7
4 hr
3 1 .0
6 hr
3 .2
8hr 2 hr
2 .8 2 2 .3
4hr
28-9
6 hr
10.7
8hr
11.3
2 hr
2 3 .5
4 hr 6 hr
30.3 1 1 .9
8 hr 2 hr 4 hr
1 3 .2 2 1 .4 3 0 .3
6 hr 8 hr
12.6 1 5 .4
TKN (m g rt.)
2 5 .7 1.6
1 7 .8 41.1 1 4 .4 1 4 .4 1 4 .4 4 3 .3 1 8 .7 11.1 1 5 .2 4 5 .6 1 6 .0 1 0 .5 2 0 .8 5 9 .3 2 5 .7 1 9 .7 2 8 .5 5 3 .4 2 7 .0 2 7 .0 2 7 .0 5 6 .2 2 7 .0 2 8 .5
Alkatfnftjr 4 33 .0 3 2 6 .0 5 3 4 .0 5 2 7 .0 3 57 .0 3 5 0 .0 5 40 .0 5 2 8 .0 3 5 5 .0 3 40 .0 5 1 1 .0 5 2 4 .0 3 5 4 .0 3 5 0 .0 5 0 7 .0 5 1 1 .0 3 8 1 .0 4 00 .0 4 93 .0 5 1 6 .0 407 .0 4 00 .0 5 1 0 .0 5 3 1 .0 4 47 .0 417 .0
File: AFFF Inhib (2-11-97).xls Sheet: Standards & Data
B-72
US00001078
A FFF R eacto r (ppm )
A1 0 A2 0 A3 0 B1 6 0 B2 60 83 60
D efoam er AF 9020
m tniter 0
0
0
15
15
15
------------------ !
Cl-
Stage
Tim e
A re a
(m g/L)
Feedstock R R Decant
7458784718 6215776781
2 8 3 .8 236.7
End of Feeding End of Anaerobic End of Aerobic
2hr 4 hr 6 hr
6521436722 6541737522 6526328166
248 3 2 4 9 .0 2484
End of Settling End of Feeding End of Anaerobic
8 hr 2 hr 4 hr
6628917720 $496185022 6493904107
2 5 2 .3 2 4 7 .3 2 4 7 .2
End of Aerobic End of Settling End of Feeding
6 hr 6607957850 8 hr 6559750264 2 hr 6462688484
2 5 1 .5 2 4 9 .7 2 4 6 .0
End of Anaerobic End of Aerobic End of Settling
4 hr 6 hr 8 hr
$541325694 6537345571 5696567822
2 4 9 .0 2 4 8 .9 2 5 4 .9
End of Feeding End of Anaerobic End of Aerobic End of Settling End of Feeding End of Anaerobic
2 hr 4 hr 6 hr 8 hr 2 hr 4 hr
6379079984 6384157598 6408351115 6738146973 6373314182 6446545211
2 4 2 .9 2 4 3 .0 2 4 4 .0 2 5 6 .5 2 4 2 .6 245.4
End of Aerobic End of Settling End of Feeding End of Anaerobic End of Aerobic End of Settling
6 hr 8 hr 2 hr 4 hr 6 hr 8 hr
6207893047 6626744880 6391628940 $401156329 6568896471 6532740605
2384 2 5 2 .3 2 4 3 .3 2 4 3 .7 250.1 248 7
Stand 3
5219091409 198.8
f N O j-r1
A re a 0 0
(m g/L) 0 .0 0 .0
0 0 142391549
0 .0 0 .0 2 .9
153197095 0 0
3.1 0 .0 0 .0
157637708 147310900
0
3 .2 3 .0 0.0
0 166524849 148840324
0 3 .4 3 .0
0 0 145107540
0.0 0.0 2 .9
126417813 0 0
2 .5 0 .0 0.0
121298643
24
134009389
2 .7
0 ' 0.0
0 109336485
0.0 2.1
131553129 917655285
2 .6 2 0 .7
N O j*-N
A re a
(m g/L)
6230008 1461239493
0 .9 2 4 .3
i 225112 206220 1158812851
0 .8 0 .8 1 9 .4
1131344921 3020068 0
1 9 .0 0 .8 0 .8
1116272031 1329426657
0
18.7 22.1 0 .8
15585084
1 .0
1194876061 1007475791
2 0 .0 1 7 .0
156248 105106 635447762
0 .8 0 .8 11.0
793675421 0
96884
1 3 .5 0 .8 0 .8
280948060 514120835
145740
5 .3 9 .0 0 .8
0 293674209
0 .8 5 .5
809881753 1148516754
13.8 1 9 .2
I ........ P04-P
A b so rb . mg/!
1.612 0 .0 1 9
4 7 .8 9 -1.24
056 0 .6 0 7 a i4 9
1 5 .4 5 16.90 2 .7 7
0.17 0 .4 8 0 .4 9
3 .4 2 1 2 .9 8 13.29
0 .0 3 9 0.151 0 .6 3 9
-0.62 2.83 17.88
0 .6 9 3 0.184 0 .2 5 2
1955 3.85 5.95
0.725 0.905 0.257
2 0 .5 4 2 6 .0 9 6.10
0.467 0 .8 8 9 1.236
12.58 25 60 3 6 .3 0
a i2 B 0 .6 1 6 0.903
2 .1 2 17.18 26 03
1.495 0.514
44 29 14.03
0.301
7.46
Standards used STD 1 STD 2 S7D 3 STD 4
472916 2592013444 5234110314 7902689639
00 100 454327901 200 925169445 300 1738139936
0 1293424 10 537513653 20 1154945353 40 2467096565
0 0l185 1C 0 .7 5 6 20 1.217 4C 1.64 5
5 20 35 50
File: A F F F Inhib (2-11-97).xls Sheet: Standards & Data
B-73
US00001079
IC Colum n N 0 2'- N S tan d ard C urve
4 .0 0 E -0 8 7.6S E -01
(71 Z0OE-O8 / -4 .1 8 E -0 1
IC Colum n N O , - N Standard Curve Phosphorus Standard Curve
Z 0 0 E -0 8 7 .8 2 E -0 1
File: AFFF Inhib (2-11-97).xls Sheet: Standards & Data
B-74
US00001080
SUMMARY OUTPUT
CL
R e g r e s s io n S tatistics
R Square
0 .9 9 9 9 5 6 4 2
O bservations
4
C oefficients S tandard E rro r
In te rc e p t X Variable 1
0 .7 6 5 1 9 1 3 6 3.7951 E -08
0 .8 7 0 4 1 2 0 7 9 1.7715E -10
SUMMARY OUTPUT
N 0 2 -N
R eg ressio n S tatistics
R S quare O bservations
0 .9 9 8 7 3 0 6 7 4
C oefficients S tandard E rre r
In te rc e p t X V a ria b ie l
-0 .4 1 7 7 2 5 8 5 2.2989E -0 8
0.58553 1826 5.79516E -10
SUMMARY OUTPUT
N 0 3 -N
R eg ressio n S tatistics
R S quare O bservations
0 .9 9 8 1 7 4 3 9 4
C oefficients S tandard E rror
In te rc e p t X V a riab lei
0 .7 8 1 6 8 7 5 1.6072E -08
0.67473 9918 4.86023E -10
SUMMARY OUTPUT
P 0 4 -P
R e z e s s io n S tatistics
R Square
0 .9 9 5 4 0 5 3 5
O bservations
4
In te rc e p t X Variable 1
O u e u e rts S tandard E rro r
-1 .8 2 3 9 5 0 6 5
1 .6 2 1 9 4 2 9 1 7
308429668
1 .4 8 1 7 2 4 6 6 4
File: AFFF Inhib (2-11-97).xls Sheet: Standards & Data
B-75
US00001081
Reactor A1 A2 A3 B1 B2 B3
AFFF (ppm )
0 0 0
60 60 60
D efo am er A F 9020
ml/Trter
Stage Feedstock
R R Decant
0 End of Feeding
End of Anaerobic End of Aerobic
E n d of Settling
0 End of Feeding End of Anaerobic
E n d of A erobic
End of Settling
0 End of Feeding
End of Anaerobic
End of A erobic
E n d of Settling
1 5 E n d of Feeding E n d of Anaerobic
E n d of Aerobic
E n d of Settling
1 5 E n d of Feeding E nd of Anaerobic E n d of Aerobic E n d of Settling
1 5 E n d of Feeding End of Anaerobic E nd of A erobic E n d of Settling
STD 1 STD 2
STD 3 STD 4
STD 5
COD
T im e 2 hr
ABS (mg/L) 0 .087 112 5.0 0.011 104.3 0 .014 144.6
% COD Removal 67 .5
4 hr 6 hr 8 hr 2 hr 4 hr 6 hr 8hr 2hr 4 hr 6 hr 8 hr 2 hr 4 hr
0 .0 1 3 0 006 0 .0 0 4 0 .0 1 4 0 .0 1 4 0 .0 0 4 0 .0 0 5 0 .0 1 2 0 .0 1 0 0 .0 0 8 0 .0 0 7 0.061 0 .0 5 7
1 3 1 .2 37.1 1 0 .3
1 4 4 .6 1 4 4 .6
1 0 .3 2 3 .7 1 17 .7 9 0 .9 6 4 .0 5 0 .6 7 75 .8 7 2 2 .1
7 0.5 9 1.6 9 7.7 67 .5 67 .5 9 7 .7 9 4 .7 73 .5 7 9.6 85 .6 8 8 .6 59 .3 62.1
6 hr 6 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr 8 hr
0.065 829.5
0 .0 7 8 1004.1
0.067 8 56 .4
0.058 7 35 .5
0.059 748.9
0.065 8 29 .5
0.061
7 7 5 .8
0.059 748.9
0 .064 816.1
0 .075 9 63 .8
0 .0 0 0 0 .0 8 3
0 1000
0 .1 5 3
2000
0 .2 2 3
3000
0 .3 3 9
4500
5 6 .5 47 .3 5 5 .1 6 1 .4 6 0 .7 5 6 .5 5 9.3 6 0 .7 5 7 .2 49 .5
F S (Filtered) FS A verage
0 .087 109 6.0 0.087 .1096.0
R R S U (F iltered ) R R SU A verage
0 .0 1 1 0 .0 1 1
5 9 .3 5 9 .3
* The values of "COD % Removal" shown in table and chart above are accumulative figures based on the initial CO D concentration at time 0 hr.
File: A FFF Inhib (2-11-97).xls Sheet: COD
B-76
US00001082
S am p le C ontrols (A 1.A 2& A 3)
In itia l COD at T im e 0 hr.
C onstituent R R Decant Feedstock D efoam er AFFF T o ta l
Vol (L)
C O D m g /L
4 104.3
2 1125.0
20
00
6
417 .1 7 2
2 2 4 9.94 6 0
0
4 4 4 .5
Inhibition (B 1.B 2& B 3)
R R Decant F e e d s to c k D efoam er AFFF T o ta l
4
1 0 4 .3
4 1 7 .1 7 2
2
1 1 2 5 .0
2 2 4 9.94 6
1 5300 5300
2 1 1737
3474
6 190 6.9
% COO Removal
A1 (0) 9 7 .7
A 2 (0 ) 9 4 .7
A 3 (0) 8 8 .6
B1 (60) 4 7 .3
B2 (60) 5 6 .5
B 3 (60) 4 9 .5
% COD Removal
A 1 (O)
A 2 (0)
A 3 (0)
B1 (60)
R eactor (A FFF ppm )
B 2 (60)
B 3 (60)
CO D Standard Curve
m = 13430 / = -4 3 .4 3 7
File: A FFF Inhib (2-11-97).xls Sheet: COD
B-77
US00001083
Dissolved Oxygen
Feeding
A naerobic STAGE
A erobic
DA1 EJA2 A 3 B1
B2
E3B3
Oxygen Uptake during Aerobic Stage
File: A FFF Inhib (2-11-97).xls Sheet: Oxygen Uptake
B-78
US00001084
Stage --------------------------
Feeding
A n a e ro b ic
A e ro b ic
Dissolved O xygen (mg/L) a t various sta g e s
A1
0.11
0 .0 7
A2 0 .0 9 0 .0 8
A3 0 .0 9 0 .0 9
B1 0 .0 9 0 .0 9
B2
0 08 0.10
4 .8 8
4 .3 0
5 .2 7
4.21
5 .2 7
B3
0.10
0 .09 4.8 5
S ta g e A e ro b ic
Tim e (sec)
0
30 60> 90
120
150 180
210
240 270 300 330 360 390 420 450 480 510 540 570 600
A1 9 .1 2 5 .9 8 5 .3 2 5 .0 9 4.91 4.7 3 4 .5 7 4.41 4 .1 7 3 .9 5 3 .7 3 3 .5 2 3.31 3 .1 0 2 .8 9 2.71 2 .5 2 2 .3 4 2 .1 8
2.01
1.85
D isso lved O xyg en in m g/L
A2 8 .9 2
A3 8 .2 8
B1
8.00
5 .5 4
5 .8 3
5 .3 8
4 .8 5 4 .5 9
5 .2 8 508
4 .8 8 4.6 6
4 .4 0 4 .2 3
4 .9 2 4.7 6
4 .4 9 4.3 3
4 .0 5
4 .60
4 .1 6
3 .8 8
4 .4 6
4 .0 1
3 .6 3
4 .2 3
3 .8 0
3.4 0
4.01
3 .5 9
3.1 7 2.9 5 2 .7 4
3 .7 9 3 .5 9 3 .3 7
3 .3 8 3 .1 7 2 .9 7
2 .5 3
3 .1 7
2 .7 7
2 .34
2 .9 7
2 .5 9
2 .1 6
2 .7 8
2.41
1 .9 8
2 .59
2 .2 3
1.81
2.41
2 .0 6
1 .6 5
2 .2 4
1 .8 9
1 .4 9
2 .0 8
1 .7 2
1 .3 3
1.92
1.57
B2 8 .2 4 6 .49 6 .0 4 5 .8 0 5 .6 1 5 .4 5 5 .2 9 5 .1 5 4 .9 3 471 451 4.31 4 .1 2 3 .9 3 3 .7 4 3 .5 8 3 .3 6 3 .2 2 306 2 .9 0 2 .7 4
B3 8.6 9 6.9 6 6.6 4 640 6.2 6
6.11
5.9 8 5.8 6 5.6 9 5.5 4 5.3 9 5 .2 5 5 .1 2 4.9 9 4.8 5 4 .74 4.6 2 4 .5 0 4.3 9 4.2 8 4.1 8
File: AFFF Inhib (2-11-97).xls Sheet: Dissolved Oxygen
B-79
US00001085
Date of Test: 3-25-97
Concentrations Table
AFFF Cone. = 60 ppm
Reactor A1 A2 A3 B1 B2 B3
AFFF (ppm )
0
0 0
60
60
60
D efo am er A F 9020
m t/liter
Stage
F e e d s to c k
R R Decant
0 E nd a f Feeding
E n d d A naerobic
E n d of A ercbic
E n d of Settling
0 E nd of Feeding
E n d of A naerobic
E n d of Aerobic
E n d of Settling
0 E n d of Feeding
E n d o f Anaerobic
E n d of A erobic
E n d of Settling
15 E n d of Feeding
E n d of Anaerobic
E n d c f A erobic
E n d of Settling
15 E n d c f Feeding
End of Anaerobic
E n d o f Aerobic
E n d of Settling
15 E n d of Feeding E n d of Anaerobic
E n d o f Aerobic
E n d o f Settling
C o n cen tratio n , m g/L
Tim e
2 hr
4 hr
TKN NH3-N
2 5 .7
26 .7
1.6 0.1
17.8
2 4 .6
41.1
3 0 .0
Org. N
- 1.0
1 .5 -6 .8
11.1
N 0 2-N N 0 3-N
0.0 0 .9 0.0 2 4 .3 0.0 0.8 0.0 0.8
Total N 2 6 .5 2 5 .8 1 8 .6 41 .9
Cl- P04-P 2 8 3 .8 47.9 2 3 6 .7 -1.2 2 4 8 .3 15.4 2 4 9 .0 16.9
6 hr 8 hr 2 hr
4 hr
14.4 14.4 14.4 4 3 .3
4 .7
2.8
24 .0 2 9.4
9 .8
11.6
-9 .6 1 3 .9
2 .9 19.4
3.1 1 9 .0
0.0 0.8 0.0 0.8
36.7 2 48 .4 36.5 2 5 2 3 15.3 2 4 7 .3 44.1 2 4 7 .2
2 .8 3 .4 13.0 133
6 hr 18.7
4 .5 14.3
3 .2 1 8 .7 4 0 .7 2 5 1 .5 -0.6
8 hr 2 hr
4 hr
11.1
15.2 4 5 .6
3 .2 2 5.7 3 1.0
8.0
-1 0 .5 14.6
3 .0 22.1
0.0 0.8 0.0 1.0
36.2 2 49 .7 16.0 2 46 .0 46.6 249 .0
28 1 7 .9 1 9 .6
6 hr 16.0
3 .2 12.8
3 .4 20.0 3 9 .4 2 4 8 .9 3 .9
8 hr 2 hr
4 hr
10.5
20.8
5 9 .3
2.8
22 .3 2 8 .9
7 .8 -1 .5 3 0 .4
3.0 17 .0
0.0 0.8 0.0 0.8
30.5 2 54 .9
21.6 2 4 2 .9
6 0 .0 2 43 .0
5 .9 2 0 .5 26.1
6 hr 25.7
1 0 .7
1 4 .9
2 .9
11.0
3 9 .6 2 4 4 .0 61
8 hr 19.7
1 1 .3
8 .5
2 .5 1 3 .5 3 5 .8 2 5 6 .5 12.6
2 hr
4 hr
6 hr
2 8 .5 5 3 .4 2 7 .0
23 .5 3 0 .3 1 1 .9
5 .0 23.1 1 5 .2
0.0 0.8 2 9 .3 2 4 Z 6 2 5 .6
0.0 0.8 5 4 2 2 4 5 .4 3 6 3
2 .4 5 .3 3 4 .7 2 3 6 .4 2.1
8 hr 2 hr
4 hr
6 hr I 8 hr
2 7 .0 2 7 .0 5 6 .2 2 7 .0 2 8 .5
1 3 .2 2 1 .4 3 0 .3
12.6
1 5 .4
13.8 5 .7 .
2 6 .0 14.5 13.1
2 .7
0.0 0.0
2.1
2.6
9 .0
0.8
0 .8 5 .5 1 3 .8
38.7 2 5 2 .3 2 7 .8 243 .3 57.0 243.7
34 6 250.1
4 4 .9 2 48 .7
17.2 2 8 .0 44 3 1 4 .0 7 .5
File: AFFF Inhib (2-11-97J.xls Sheet: Concentrations Table
B-80
US00001086
D ate of Test: 3-25-97
Concentrations T ab le
A FFF Cone. = 60 ppm
Reactor A1 A2 A3 B1 B2 B3
AFFF (ppm )
Stage
Feedstock
RR Decant
0 End of Feeding
End of A naerobic
End of A erobic
E n d of Settling
0 End of Feeding
End of A naerobic
E nd of A erobic
E n d of Settling
0 E nd of Feeding
End of Anaerobic
E nd of A erobic
E n d of Settling
6 0 E nd d Feeding End of Anaerobic
E n d of Aerobic
E n d of Settling
6 0 E n d of Feeding End of A naerobic
E n d of A erobic
E n d of Settling
60 E n d of Feeding
End of A naerobic
E n d of Aerobic E n d of Settling
FS1
FS2
FS3
FS Avarage
RRSU1 RRSU2
RRSU3 R R S U Avarage
Tim e
2 hr
4 hr
6 hr 8 hr 2 hr
4 hr
6 hr 8 hr 2 hr
4 hr
6 hr 8 hr 2 hr
4 hr
6 hr 8 hr 2 hr
4 hr
6 hr 8 hr 2 hr
4 hr
6 hr 8 hr
C o n c e n tra tio n , m g /L
BOD
COD 11 2 5 .0
TOC 4 4 3 .1
1 04 .3 1 44 .6
1 3 .9 2 5 .4
1 31 .2 3 7 .1
1 9 .1 1 5 .2
1 0 .3 144 .6
1 5 .3 2 5 .4
1 44 .6 1 0 .3 2 3 .7
1 8 .3 1 4 .4 1 4 .4
1 1 7 .7 9 0.9 6 4.0 50 .6
2 4 .0 17.7 14.4 14.2
7 7 5 .8 7 2 2 .1 8 2 9 .5 1 0 0 4 .1
2 4 1 .1 2 2 5 .9 2 47 .5 3 2 6 .0
856 .4 7 3 5 .5 748 .9 8 2 9 .5 775 .8 748 .9 8 1 6 .1 963 .8
2 4 9 .6 2 3 5 .8 2 3 2 .6 2 4 6 .2 2 4 7 .4 2 2 9 .4 2 36 .2 2 7 6 .4 4 4 4 .1
4 43 .6
441 .5
4 4 3 .1 14.7
1 3 .5
1 3 .3 1 3 .8 7
| Alkalinity H C 0 3 (mg/l) 4 3 3 .0 326 .0 534 .0 527 .0 357 .0 3 50 .0 540 .0 528 .0 355 .0 340 .0 511 .0 5 24 .0 354 .0 3 50 .0 5 07 .0 5 11 .0 3 81 .0 400 .0 493 .0 5 16 .0 4 0 7 .0 400 .0 5 10 .0 531 .0 447 .0 417 .0
TOC -vs.- COD
File: A FFF Inhib (2-11-97).xls Sheet: Concentrations Table
B-81
US00001087
AFFF Nitrification Inhibition in tennis of NH3-N
Inhibition Effect of AFFF on Nitrification in terms of N03'-N
Inhibition Effect of AFFF on Nitrification in terms of N02-N
File: A FFF Inhib (2-11-97).xls Sheet: NH3-N & TKN Charts
T im e
B-82
US00001088
Inhibition Effect o f AFFF on Nitrification in terms of TKN Inhibition effect of AFFF on Phosphorus removal
File: A FFF Inhib (2-11-97).xls Sheet: NH3-N & TK N Charts
B-83
US00001089
Date o f Test: 3-25-97
Inhibition Reactors / Nitrogen Mass Balance
A FFF Cone. = 60 ppm
50
40 o>
Ec. 3 0
tt>
g 20
I 10 0
Reactor A1 (No AFFF Present)
fzriitr
'ljT
End of F e e d in g
n^;
-3 '
'Kih*
li Mi
End of A n a e ro b ic
End of A e ro b ic
STAGE
End cf S e ttlin g
N 0 2 -N N 0 3 -N H O rg. N 0 N H 3-N
Reactor A2 (No AFFF Present)
N 0 2 --N N 0 3 -N B O rg .N N H 3-N
End cf F e e d in g
End cf A n a e ro b ic
End of A e ro b ic
STAGE
End of S e ttlin g
Reactor A3 (No AFFF Present)
N 0 2 -N N 0 3 -N H O rg. N O N H 3 -N
STAGE
I
File: A FFF Inhib (2-11-97).xls Sheet: Nitrogen Mass Balance
B-84
US00001090
D ate of Test: 3-25-97
Inhibition Reactors / Nitrogen M ass Balance
AFFF Cone. = 60 ppm
Reactor B11 60 ppm AFFF with Defoamer AF 9020) 7U -
j 60 ' |> 5 0
c^ o> 3 0 ao 2 0 z
10 -
|5 |
0 ----
End of F eeding
HP
End of A n a e ro b ic
End of A ero bic
s t ;\G E
W-
N 0 2 -N N O S -N B O rg. N N H 3-N
E nd cf Settfing
Reactor B2 (60 ppm AFFF with Defoamer AF 9020)
N 0 2 -N N O S -N B O rg .N D N H 3-N
End of F e e d in g
End of A n a e ro b ic
End of A erobic
STAGE
E n d of S e ttlin g
File: A FFF Inhib (2-11-97).xls Sheet: Nitrogen Mass Balance
B-85
US00001091
R eactor A1
AFFF ppm
0
(ControO
A2 (ControO
0
A3 (ControO
0
B1 60
B2 60 B3 60
D efo am er A F 9020
0 0 0
15
15 15
S tag e E n d of Feeding E n d of A naerobic E n d of A erobic E n d of Settling E n d of Feeding E n d of A naerobic E n d of A erobic E n d of Settling E n d of Feeding E n d of Anaerobic E n d of A erobic E n d of S ettling E n d of Feeding E n d of Anaerobic E n d of A erobic E n d of Settling E n d of Feeding E n d of A naerobic E n d of Aerobic E n d of Settling E n d of Feeding E n d of Anaerobic E n d of A erobic E n d of Settling
Tim e
2 hr
4 hr
6 hr 8 hr 2 hr
4 hr
6 hr 8 hr 2 hr
4 hr
6 hr 8 hr 2 hr
4 hr
6 hr 8 hr 2 hr
4 hr
6 hr 8 hr 2 hr
4 hr
6 hr 8 hr
Nitrogen Concentration, mg/L
N H 3-N N 0 3 -N
N 0 2 - N A ( N 0 3-N )
2 4 .5 5
0.8
0.0
3 0 .0 2
0.8
0.0
A (N O j-N )
4 .6 7
1 9 .4
2 .9
2 .8 2
1 9 .0
3.1 1 8 .1 6
3 .1 0
2 3 .9 7 2 9 .4 4
0.8 0.8
0.0 0.0
4 .4 5 3 .1 5 2 5 .6 5 3 0 .9 7 3 .2 0
1 8 .7
22.1 0.8 1.0
20.0
3.2 3.0 2 1 .3 2
0.0 0.0
3 .4
2.9 7
2 .7 8 2 2 .3 1
1 7 .0
0.8
3.0 16.19
0.0
3 .0 0
2 8 .8 8 10.74
0.8 11.0
0.0
2 .9
1 1 .2 7 2 3 .5 0 3 0 .2 6
1 3 .5
0.8 0.8
2.5 12.75
0.0 0.0
2.4 9
1 1 .8 5 13.21 2 1 .3 5 3 0 .2 6 1 2 .5 7 1 5 .3 7
5 .3 9 .0
0.8 0.8
5 .5 1 3 .8
2 .4
2.7 8 .2 6
0.0 0.0 2.1 2.6 1 3 0 1
2.66
2 .6 1
Ammonia Remaining (Supematent)
1 5 .0 0
12.00
-J
f 9.00
\x 6.00
z 3.00
0.00 -
A 1 '
A2
A3
B1
B2
B3
Nitrate (in Supernatant)
Rie: AFFF Inhib (2-11-97).xls Sheet: N 0 3 - & N 0 2 Produced
B-86
i US00001092
File: AFFF Inhib (2-11-97).xls Sheet: N03- &N02 Produced
B-87
US00001093
BNR Inhibition T ests - 60 ppm AFFF Pretreated w'rth Defoamer AF9020 (Nov/2/97)
TRSeSactor AAABBB112323
Intial wt 111...111111222163 111...111223020759
Fi1n.1al57wt 111...111557641659 11..11782552
.
Volume 1155 11115555
MLSS 22999333 2333843611323330
T @ 550 111...11111176737 111..11.122386714
MLVSS 222665622700 323092361303
TDS
AR1eactor AA23 BBB123
Iriitial wt 11..00126397 1.024 111...000323657357
F1i.n0a3l3w8 t 11.0.033888 111...000545618157
Volume 1155 15 111555
T11D2S7 1007 933 111304260700
ATAARB1S123eactor BB23
In11i..t00ia11l46w67 t 1111....0000212247681930
F11111i1...n..0001.11a77700l0571944w95792t
Vo1l11111u555555me
444555T001110S853788733300
TSS,TDS 43192400 3445774043027300
File: AFFF Soilds Data
B-88
.
I
US00001094
BNR Inhibition Batch Assay Pretreated with Best Performing Defoamer (A FFF 120 ppm)
B-89
US00001095
Reactor A1
A2 B1 B2
C1 C2
AFFF (ppm )
0
0 0 0
120 120
D o foam er A F 9 0 2 0 m l/iiter
Stage
Feedstock R R Decant
0 E n d o( Feeding
E n d of Anaerobic E n d of Aercbic Extended Aeration
E n d of Settling
0 E n d of Feeding
E n d of Anaerobic
E n d of Aerobic E n d of Settling 3 0 E n d of Feeding
E n d of Anaerobic
E n d o f Aerobic E n d of Settling
3 0 E n d o f Feeding E n d of Anaerobic E n d of Aerobic
Extended Aeration E n d o f Settling 3 0 E n d of Feeding
E n d of Anaerobic E n d of Aerobic E n d of Settling
3 0 E n d of Feeding E n d of Anaerobic E n d of Aerobic
Extended Aeration E n d o f Settling
Tim e
N H 3-N (m g/L)
TKN (m g/L)
2 hr
3 2 .2
0.1
2 6 .3
8 0 .7 1 1 .9 6 7 .9
4 hr
6 hr
(4hr)
8 hr 2 hr
4 hr
6 hr 8 hr 2 hr
31.1 2 .3
0.1 0.0
2 3 .3 2 8 .7
2 .9
2.2
2 0 .7
55.1 15.6
7 .7 5 .7 64.1 52.1 1 3 .8 1 7 .5 57.1
4 hr
6 hr 8 hr 2 hr
2 8 .7 3 .9 2 .9
1 9 .9
4 9 .3 19.7 14.1
101.8
4 hr
6 hr
<4hr)
8 hr 2 hr
4 hr
6 hr 8 hr 2 hr
4 hr
6 hr
2 8 .7 3 .5
0.1 0.1
2 0 .7 3 1 .0 11.5
6.6
19.9 2 9 .8 1 3 .0
55.1 18.6
8.0
6.4 1 7 1 .3
81.4 29.8 16.7 8 5 .5 9 6 .2 15.6
(4hr)
8 hr
0 .0 5 0 .0 7
1 3 .3 4 17.7
A lk a lin ity 4 93 .0 2 9 5 .0 4 83 .0 4 62 .0 3 2 2 .0 2 90 .0 2 99 .0 4 68 .0 5 13 .0 2 99 .0 3 05 .0 4 83 .0 4 62 .0 3 6 2 .0 3 5 5 .0 4 77 .0 5 0 1 .0 3 35 .0 3 1 0 .0 3 1 6 .0 4 44 .0 490 .0 3 9 6 .0 3 87 .0 4 72 .0 4 37 .0 4 0 0 .0 294 3 1 1 .0
File: AFFF Inhib (10-12-97J.xls
Sheet: Standards & Data
B-90
US00001096
R e a cto r A1
A2 B1 B2
Ci C2
A FFF (ppm )
0
0 0 0
120 120
Defoam er A F9020 m tfllter 0
0 30 30
30 30
C l-
NCV-N
N<V-N
P04-P
Stage
Tim e
A re a
(m g/L)
A re a
(m g/L)
A re a
(m g A .) A bso rb .
Feedstock R R Decant End of Feeding
7054379476 271.7
0
5734515761 221.3 16370108
2 hr 6348421565 244.8
0
0.0 0.4 0.0
9902431 996533663
3849770
1.1 17.7 0 .9
0.366 0.052 1.351
Ind of A naerobic
4 hr 5994018561 231.3
0
0.0 4797019 1.0
End of Aerobic
6 hr 6169545264 238.0 271036986 6.4 1126185695 19.9
Extended Aeration (4hr) 6267692633 241.7
0
0.0 1787334663 31.1
End of Settling
8 hr 6311578450 243.4
0
0.0 1785261984 31.1
End of Feeding End of A naerobic
2 h r 6202705931 239.2 4 hr 6191501229 230 8
0 0
0.0 2350208 0.9 0.0 184122 0.9
1.313 0.529 0.258 0.241 1.418 1.513
End of Aerobic End of Settling End of Feeding
6 hr 6255671833 241 2 266251370 6.3 1079785510 19.2
8 hr 6208634134 239.4 241528645 5.7
874891000 15.7
2 hr 430780220 2 263
0
0.0 1660856 0.9
0.368 0.446 1.807
End of A naerobic End of Aerobic End of Settling End of Feeding End of Anaerobic End of A erobic Extended Aeration End of Settling E n d of F eed in g End of A naerobic E n d of A erob ic End of Settling E n d of Feed ing E n d of A n a ero b ic E n d of A ero b ic Extended Aeration End of Settling
stand 2 Stand 3 stand 4
4 hr 6hr 8 hr 2 hr 4 hr 6 hr (4hf) 8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6hr (4hr) 8 hr
420167237 221.4
0
424214043 223.3 15462091
440646369 230.8 17074983
412011695 217.7 1158193
413015053 442187880
218 2 231 5
2150122 16477017
489608591 244.1 2709918
446746147 233 6 2538866
429190296 225.6
0
423750052 439728579
223.1 230 2
0 8380591
425790596 224 0 9741585
407279466 215.5
729742
414717322 421088553
218 9 2 2 1 .9
0 8343067
429931641 225.9 4392062
441428568 231.2 2962652
2472225244 96.80 427056958
5095839709 7600127427
1 9 7 .0 2 9 2 .5
871460063 1 .6 4 4 E + 0 9
0.0 4.4 4 .9 0.1 0.1 4.7 0 .8 0.7 00 0 .0 24 2 .8 01 0 .0 2 .4 13 0.8 10.15 20.7 39.1
2433724 50983819 52814085
0 .9 20 .9 2 1 .3
4824306 3879584 56288014
1 .0 0 .9 2 2 .0
90396623 84010344 1619044
2 9 .0 2 7 .7 0 .9
3811496 40313185 39107556
0 .9 18 8 18 5
1841106
09
17034729 29085719
1.2 16.5
75586351 86642505
2 5 .9 28 2
9 .3 3
1087463930 2320505088
1 9 .3 4 0 .2
1.628 0 .4 4 8 0 .3 7 5 1.085 1.708 0 688 0 .5 8 8 0.603 1.595 1.642 0.966 0 934 1.658 1.747 0 8B1 0.369 0.616
Standards used
STD 1 STD 2 STD 3 STD 4
3257406 2528446248 504325692 7B8790023S
CC 10C 425265034 20C 861191951 30C) 1 .6 7 2 E + 0
C 103431C 1C 4 9 7 5 9 68 9 5 2C 1 0 9 0 4 88 6 7 E 4( 2338151857
C 0.156 1C 0 .624 2C 1 .11 3 4C 1.59 8
m g/l 11.71 1.93 4 2 .4 0 41.21 16.79 8 .3 5 7 .8 2 4 4 .4 8 4 7 .4 4 1 1 .7 8 14.21 5660 5 1 .0 3 1427 1200 3411 5352 2 1 .7 5 1 8 .6 3 1910 5 0 .0 0 5 1 .4 6 3040 29 41
5196 5 4 .7 3 2 7 .7 6
11.8 1 9 .5 0
5 20 35 50
Rie: AFFF Inhib (10-12-97).xls Sheet: Standards &Data
B-91
US00001097
IC C olum n CI" S tan d ard Curve
c r C one, mg/l IC Colum n N 0 2"- N S tandard Curve
4.0OE -08 241E+00
200E-C 8 -1 .8 5 E -0 1
IC Colum n N 0 3 - N Standard Curve
2.00E -03 8 .8 3 E -0 1
Phosphorus Standard Curve
File: A F F F Inhib (10-12-97).xls Sheet: Standards & Data
B-92
US00001098
SUMMARY OUTPUT
CL
Regression Statistics
R Square O bservations
0 .9 9 9 0 0 6 7 0 4 4
Coefficients Standard Error
In te rc e p t
2.41 2 2 7 5 6 4 2 3 .95777 1334
X V ariable 1
3.81786E -08 8.16224E -10
SUMMARY OUTPUT
N 0 2 -N
R egression Statistics
R Square
0 .9 9 9 7 2 2 1 6 5
O bservations
4
Coefficients Standard Error
In te rc e p t
0 0.27175 3644
X V ariable 1
2.37 6 4 7 E -0 8
2.81876E -10
SUMMARY OUTPUT
N 0 3 -N
R egression Statistics
R Square
0 .9 9 7 7 S 4 5 2 1
O bservations
4
Coefficients S tandard Error
In te rc e p t X Variable 1
0 .6 8 2 8 6 9 3 2 9 1 .6 9 2 4 9 E -0 8
0740861883 5.6393E -1 0
SUMMARY OUTPUT
P D 4-P
R egression Statistics
R Square
0.99991 0723
O bservations
4
Coefficients Standard Error
In te rc e p t X Variable 1
0 .3 1 3 9 5 3 7 8 2 3 1 .1 4 9 8 6 6 7 6
021342196 0208127792
File: A FFF Inhib (10-12-97).xls Sheet: Standards & Data
B-93
US00001099
Reactor A1
A2 81
B2
C1 C2
AFFF (ppm )
0
0
0
0
120 120
*
D efo am er A F 9020 Stage
Feedstock RR Decant
0 E n d o( Feeding
End of Anaerobic E n d of Aerobic Extended Aeration E n d of Settling
0 End of Feeding
E nd of Anaerobic End of A erobic End of Settling 3 0 E nd of Feeding End of A naerobic E n d of A erobic E n d of Settling 3 0 End of Feeding End of Anaerobic E n d of Aerobic Extended Aeration E n d of Settling 3 0 End of Feeding End of Anaerobic End of Aerobic End of Settling 3 0 E n d of Feeding End of Anaerobic E n d of Aerobic E nd of Anaerobic E n d of Settling
STD 1 STD 2 STD 3 STD 4 STD 5 F S (Filtered) F S (Filtered) FS Average R R S U (Filtered) R R S U (Filtered) R R S U A verage
T im e
2 hr
4hr
6 hr
<4hr)
8 hr 2 hr
4 hr
6 hr 8 hr 2 hr
4 hr
6 hr 8 hr 2 hr A hr 6 hr
(4hr)
8 hr 2 hr
4 hr
6hr
8 hr 2 hr A hr 6 hr
4 hr
8 hr
COD
ABS (mg/L)
0 .0 8 6 1102.0
% COD Removal
0 .0 0 3 0 .0 0 7
0.012
0 .0 0 3
3 8 .4 8 9 .7 1 5 3 .8 3 8 .4
7 7 .2 6 0 .9 9 0 .2
0 .0 0 5 0 .0 0 3 0 .0 0 5
64.1 3 8 .4 64.1
8 3 .7 9 0 .2 8 3 .7
0 .0 0 7
0.012
8 9 .7 1 5 3 .8
7 7 .2 6 0.9
0 .0 0 9 115.3
7 0 .7
0.067 8 58 .5
3 2 .7
0 .0 6 0 0 .0 5 2
7 6 8 .8 6 6 6 .3
3 9 .8 4 7 .8
0 .0 4 8 0 .0 7 3
6 1 5 .1 9 3 5 .4
5 1 .8 2 6 .7
0 .0 8 3 0 .0 5 6
0 .0 6 0 054 0.171 0 .1 6 0 0 .1 5 7 0 .1 9 4 0 .1 8 6
1 0 6 3 .6 7 1 7 .6 7 6 8 .8 6 9 2 .0
2191.2 . 2 0 5 0 .2
2011.8
2 4 8 5 .9 2 3 8 3 .4
16.7 4 3 .8 3 9 .8 4 5 .8 9 .0 1 4 .9 16.5 -3 .2
1.0
0.154 1973.4 0.161 2063.1 0.191 2 4 4 7 .5
0.202 2 5 8 8 .4 0.000 0 0 .0 7 9 1000 0 .1 6 7 2000
18.1 14.3
- 1.6
-7 .5
0 .2 4 2 0 .3 4 0
3000 4500
0 .0 9 5 0 .0 7 6 0 .0 8 6
0.001
1 2 1 7 .3 9 73 .9
1 0 9 5 .6
12.8
0 .0 0 5
64.1
0 .0 0 3
3 8 .4
* The values of "CO D % Removal" shown in table and chart above are accumulative figures based on the initial COD concentration at time 0 hr.
File: A F F F Inhib (10-12-97).xls Sheet: C O D
B-94
i
t
US00001100
S a m p le C o n tro ls (A 1.A 2)
In itia l C O D a t Tim e 0 hr.
C onstituent R R Decant F e e d s to c k AFFF T o ta l
V d (L )
C O D m g/L
4 38.4
2 1102.0
00
6
1 5 3 .7 6 8 2 2 0 4.00 8
0
3 9 3 .0
Inhibition (B 1.B 2)
R R Decant F e e d s to c k D efoam er T o ta l
4
38.4
1 5 3 .7 6 8
2
1102.0
2 2 0 4.00 8
5300
5300
6 1276.3
(C 1.C 2)
Reactor (AFFF ppm ) % COO Removal
A1 (0) 9 0 .2
R R Decant F e e d s to c k AFFF
Defoamer
T o ta l A 2 (0 ) 7 0 .7
4
2 2 1 6
B1 (0) 5 1 .8
3 8 .4
1102.0
3396 5300
B 2 (60) 458
1 5 3 .7 6 8 2 2 0 4.00 8
6792 5300 2 4 0 8 .3 C 1 (60) -3 .2
C 2 (60) -7 .5
% COD Removal
70.7
458
A 2 (0 )
B1 (0)
-H -- F"
B2 (60)
C tS O )
R eactor (A FFF ppm )
COD Standard Curve for High Range Vials
File: AFFF Inhib (10-12-97).xls
Sheet: COD
B-95
US00001101
Dissolved Oxygen
DA1 HA2 OB1 OB2 D01 EJC2
File: A FFF Inhib (10-12-97).xls Sheet: Oxygen Uptake
B-96
US00001102
S ta g e F e e d in g A n a e ro b ic Aerctoic
Dissolved Oxygen (mg/L) at various stages
A1 A2 B1 B2 C1
0.11
0 .0 7 5 .5 8
0.0 9 0.0 8 5 .8 4
0 .0 9 0 .0 9 6 .1 7
0 .0 9 0 .0 9
6.10
0.0 8
0.10
5 .5 7
C2
, 0.10
0 .09 5 .8 5
S tage A ero bic
Tim e (sec)
0
30 60 90
120
150 180
210
240 270 300 330 360 390 420 450 480 510 540 570 600
A1
8.02
6 .4 9 5 .8 6 5 .5 0 5 .1 8 4.9 0 4 .6 6 4.4 0 4 .1 8 3 .9 8 379 3 .6 0 3 .4 3 3 .2 7 3 .1 0 2 .9 5 2 .8 3 2 .6 9 2 .5 6 2 .4 4 2 .3 2
D isso hed O xygen in m g/L
A2 8 .4 3
B1 7 .2 0
B2 7.61
5 .7 7 5 .12
5 .4 2 4 .9 6
6 .2 6 5.72
4.6 8 4 .32
4 .5 4 4 .2 5
5 .3 5 4 .9 8
4 .02 3 .7 4
3.97 3 .7 3
4 .6 7 4 .3 7
3 .4 7
3.51
4.11
323 2 .9 9 2 .7 7 2.5 8 2 .3 8
2.22
_
3.27 3 .0 7
2.88
2.71 2 .5 4 2 .3 9
3 .8 3 3 .5 8 3 .3 4 3 .1 3 2.91 2 .7 2
2.0 5 1 .8 9
2.24
2.10
2.51 2 .3 2
1 .7 5 1.63 1 .4 8 1.37 1.27
1.97
1.86
1.74 1.63 1.54
2 .1 6 1.98 1.85
1.68
1.55
C1 7 .5 6 6 .3 7 5 .7 7 5 .3 1 4.9 0 4 .52 4 .17 3 .8 4 3 .5 4 3 .2 5 2.9 8 2.7 3 2 .4 8 2 .2 6 2 .0 4 1.84
1.66
1 .4 8 1 .2 9 1 .1 5
1.01
C2 8 .2 5 638 5 .6 6 5 .1 6 4 .76 4.3 5 4 .06 3.71 3 .3 9 3.11 2 .8 3 2.5 9 2 .3 6 2 .1 3 1 92 1.72 1.54 1.38
1.21
1.06 0 .9 2
File: A FFF Inhib (10-12-97).xls Sheet: Dissolved Oxygen
B -9 7
US00001103
Reactor A1
A2 B1
B2
C1 C2
A F FF D efoam er AF902C>
(ppm )
Stage
Tim e T K N
Feedstock
80.7
R R Decant
11.9
0
0
E n d of Feeding
2 hr 67.9
E nd of A naerobic E n d o f Aerobic
4 hr
6 hr
55.1 15.6
Extended Aeration (4hr)
7 .7
E n d of Settling
8 hr
5 .7
0
0
E n d of Feeding
2 hr 64.1
E n d of Anaerobic
4 hr 52.1
E n d of Aerobic
6 hr 13.8
E n d o f Settling
8 hr 17.5
0
30
E n d of Feeding
2 hr 57.1
E n d of Anaerobic
4 hr 4 9 .3
E n d o f Aerobic
6 hr 19.7
E n d o f Settling
8 hr 14.1
0
30
E n d of Feeding
2 hr 1 0 1 .8
E n d of Anaerobic
4 hr 55.1
E n d of Aerobic
6 hr 18.6
Extended Aeration (4h r)
8 .0
E n d of Settling
8 hr
6 .4
120
30
E n d of Feeding
2 hr 171.3
End of Anaerobic
4 hr 81.4
120
End of Aerobic
6 hr 29 .8
End of Settling
8 hr 16.7
30
E n d of Feeding
2 hr 8 5 .5
E n d cf Anaerobic End of Aerobic
4 hr
6 hr
9 6 .2 1 5 .6
Extended Aeration End of Settling
4hr>
8 hr
1 3 .3 1 7 .7
NH3-N 32.19 0 .11 2 6 .3 3 3 1 .0 5 2 .3 4 0 .0 5 0 .0 1 2 3 .3 4 2 8 .67
2.86
2.1 9 20.6 9 2 8 .67
3 .9 3 2.8 9 1 9 .8 8 2 8 .6 7 3 .49 0.0 5 0 .0 9 2 0 .69 3 1 .00 1 1 .5 4 6 .6 4
1 9 88
2 9 .8 4 1 3 .0 0
0 .0 5 0 .0 7
Org. N 48.5 1 1 .8 41.5 2 4 .0 13.2 7 .6 5 .7 4 0 .7 2 3 .4 1 1 .0 15.3 3 6 .4 2 0 .6 1 5 .8 1 1 .2 8 1 .9
2 6 .4 15.1
8 .0 6 .3 1 5 0 .6 5 0 .4 18.3
10.1
6 5 .7 6 6.3 1 5 .6 1 3 .3 1 7 .6
C o n e e n tra tio n , mg/L
N 0 2 -N N 0 3 -N Total N
O.C 0 .4
0.0 0.0
6 .4
0.0 0.0 0.0 0.0
6 .3 5 .7
0.0 0.0
4 .4
1 .1 17.7
0 .9 1 .0 1 9 .9 31.1 3 1 .1 0 .9 0 .9 1 9 .2 1 5 .7 0 .9 0 .9 2 0 .9
81.8 30.C
68.8
56.0
2 7 1 .7 271 3 2 4 4 .8 2 3 1 .3
1 1 .7
4 2 .4 4 1 .2
4 2 .0 3 8 .8 3 6 .8
2 3 8 .0 2 4 1 .7 243 4
1 6 .8 8 .4 7H
6 5 .0 5 3 .0 3 9 .3 3 9 .0
239 .2 2 38 .8 241 .2 2394
4 4.5
4 7 .4
11.8 1a 7
5 8 .0 5 0 .2 45.1
2 26 .3 2 2 1 .4 223 .3
5 6 .6 5 1 .0 1 4 .3
4 .9 21 3 40.3 2 3 0 8
0.1 1 .0 10 2 .8 2 1 7 .7 34.1
0.1 0 .9 56.1 2 1 8 .2 5 3 .5 4 .7 2 2 .0 45.3 2 3 1 .5 21.7
0 .8 2 9 .0 37.7 244.1 18.6
0 .7 2 7 .7 3 4 .8 2 3 3 6 1Q 1 0.0 0 .9 1 7 2 .2 2 2 5 .6 5 0 .0 0.0 0 .9 8 2 .3 2 2 3 1 5 1 .5
2.4 18.8 51.0 2 3 0 .2 30.4
2 8 18.5 38.0 224 O
0.1 0 .9 8 6 .5 2 1 5 .5 5 2.0
0.0 1 .2 9 7 .3 2 1 8 .9 5 4 7
2 .4
1 6 .5
4 7 4 2 2 1 .9 27.757
1.3 2 5 .9 4 0 .5 2 2 5 .9 1 1 .8 0 8
08
2 8 .2
46.7 2 3 1 .2 19.502
File: A FFF Inhib (10-12-97).xls Sheet: Concentrations Table
B -9 8
US00001104
Reactor A1
A2
B1 B2
Cl C2
AFFF <ppm>
0
0 0 0
120 120
D efo am er A F 9020
Stage
Tim e
F e e d s to c k
R R Decant
0
E n d cf Feeding
2 hr
E n d of A naerobic
4 hr
E n d of A erobic
6 hr
E x te n d e d A eration <4hr)
E n d of Settling
8 hr
0
E n d of Feeding
2 hr
E n d of Anaerobic
4 hr
E n d of A ero bic
6 hr
E n d of S ettling
8 hr
30
E n d of Feeding
2 hr
E n d of Anaerobic
4 hr
E n d of A erobic
6 hr
E n d of Settling
8 hr
30
E n d of Feeding
2 hr
E n d of Anaerobic E n d of A erobic
4 hr
6 hr
Exten ded Aeration E n d of Settling
(A h i)
8 hr
30
E n d of Feeding
2 hr
End of Anaerobic
4 hr
E nd of A erobic
6 hr
E n d c f Settling
8 hr
30
E nd of Feeding
2 hr
E n d of Anaerobic E nd of A erobic
4 hr
6 hr
E xten ded Aeration E nd of Settling
(4hr)
8 hr
FS1
FS2
FS3
FS Avarage
RRSU1
RRSU2
RRSU3
R R S U Avarage
C o n c e n tra tio n , m g/L
BOD
COO
1102.0
3 8 .4
TOC 3 81 .7
12.8
8 9 .7
2 5 .4
1 5 3 8 18.04
3 8 .4
13.4
64.1 14.42
3 8 .4
1 3 .9
64.1
2 4 .8
89.7 17.57
1 5 3 .8
1 3 .3
1 1 5 .3
1 1 .5
8 58 .5 241.3
7 6 8 .8 208.2
6 6 6 .3 118.8
615.1 1 97 .8
935.4 271.3
1063.6 2 33 .4
7 17 .6 139.9
7 6 8 .8 692 .0
1 31 .9
202.0
21 9 1 2 568.1
2 0 5 0 .2
2011.8
5 25 .2 5 3 4 .4
2 4 8 5 .9 2383 4 1 9 7 3 .4
6 34 .3 6 3 5 .8 5 1 4 .4
2063.1 5 4 8 0
244 7 .5 2588 4
6 5 1 .4 6 5 5 .1
3 8 0 .7
3 84 .0
3 80 .4
3 8 1 .7
13.8
12.2
1 2 .3
1 2 .8 0
j Alkalinity H C 0 3 (m g/I) 430 .0 319 .0 5 01 .0 4 7 3 .0 287 .0 297 .0 271 .0 506 .0 4 8 4 .0 2 7 3 .0 291 .0 473 .0 4 8 7 .0 294 .0 300 .0 592 .0 554 .0 475 .0 457 .0 433 0 5 84 .0 5 60 .0 487 0 5 03 .0 578 0 5 64 .0 485
477
File: AFFF Inhib (10-12-97).xls Sheet: Concentrations Table
' B-99
US00001105
Inhibition effect o f AFFF on Nitrification as o f NH3-N (for Extended 2hr Aerated Reactors)
File: A F F F Inhib (1CM2-97).xls Sheet: NH3-N & TKN Charts
B-100
US00001106
Inhibition effect of A F F F on Phosphorus rem oval (for Extended 2hr Aerated Reactors)
File: A F F F Inhib (10-12-97).xls Sheet: NH 3-N & TKN Charts
B-101
US00001107
In h ibition e ffe c t o f AFFF on N itrific a tio n as o f N H 3-N Inhibition effect of AFFF on Nitrification as of N 03-N Inhibition effect of AFFF on Nitrification as of TKN
File: A F F F Inhib (10-12-97).xls Sheet: NH3-N & TKN Charts
B-102
US00001108
in h ibition effe ct o f A FFF o n Phosphorus rem oval
File: A F F F Inhib (10-12-97).xls Sheet: N H 3-N & TKN Charts
B-103
US00001109
70.0 0 6 0 .00 I 50.0 0 - c 4 0 .0 0 |> 3 0 .0 0 -- 20.00
Z 10.00
0.00
Reactor A1 (No AFFF present)
End of F eeding
End of
End of
A n a e ro b ic
A e ro b ic
S tag e
E n d of S e ttlin g
D N 0 3 -N Q N 0 2 -N
IO rg -N O N H S -N
Reactor A2 (No AFFF present)
?i : E n d of A n a e ro b ic
S tage
0 N 0 3 -N N 0 2 -N
lO rg . N N H 3-N
R e a c to r B 1 (No A FFF p rese n t BUT w ith D efo am er A F9020)
10.00
0 N 0 3 -N N 0 2-N O rg. N ID N H 3-N
End of
End of
A n a e ro b ic
A erobic
S tag e
End of S e ttlin g
File: A FFF Inhib (10-12-97).xls Sheet: Nitrogen Mass Balance
B-104
US00001110
File: A F F F Inhib (10-12-97).xls Sheet: Nitrogen Mass Balance
B-105
US00001111
R eactor A1 (C o n tro l)
A2 (C o n tro l) B1
B2
C1
C2
AFFF ppm
0
0 0 0
120 120
WT
D efoam o r A F 9020 m !/lrter
0
0
30
30
30 30
S tag e E n d of Feeding E n d of A naerobic E n d cf A erobic E xtended Aeration E n d c f SeltTing E n d erf F e e d in g E n d of Anaerobic E n d of Aerobic E n d of Settling E n d of Feeding E n d of A n aerobic E n d of Aerobic E n d of Settling E n d of Feeding E n d of Anaerobic E n d of Aerobic Exten ded Aeration E n d of Settling E n d of F ee d in g E nd of Anaerobic E n d of Aerobic E n d of Settling E n d of Feeding E nd of Anaerobic E n d of Aerobic E m ended Aeration [E n d of Settling
Tim e
2 hr
4 hr
6 hr
(4hr)
8 hr 2 hr
4 hr
6 hr 8 hr 2 hr
4 hr
6 hr 8 hr 2 hr
4 hr
6 hr
(4 h r)
8 hr 2 hr
4 hr
6 hr 8 hr 2 hr
4 hr
6 hr
(4hr)
| 8 hr
N itro g en C o n cen tratio n , m g /L
N H 3-N N 0 3 -N
N Q 2 - N A ( N 0 3'- N )
2 6 .3 3
0 .9
0.0
3 1 .0 5 1.0 0.0
2 .3 4 0 .0 5
0.01
2 3 .3 4 2 8 .6 7
2.86
1 9 .9 31.1 31.1
0 .9 0 .9 1 9 .2
6 .4
0.0 0.0 3 0 .1 5 00 0.0
6 .3
2 .1 9 2 0 .6 9 2 8 .6 7
1 5 .7 0 .9 0 .9
5.7 1 4.77
0.0 0.0
3 .9 3
2 0 .9
4 .4
2 .8 9 19.88 2 8 .6 7
2 1.3
1.0
0 .9
4.9 20.41
0.1 0.1
3 .4 9 0 .0 5
22.0
29 .0
4 .7
0.8
0 .0 9 2 0 .6 9 3 1 .0 0 11.54
5 64 19.88 2 9 .8 4
13
2 7 .7 0 .9 0 .9
18.8 18.5
0 .9
1.2
16.5
0.7 2 6 .6 9
0.0 0.0
2.4
2.8 1 7 .8 7 0.1 0.0
2.4
0 .0 5 0.07
2 5 .9 2 8 .2
1.3
0.8 2 7 .2 8
Am m onia Remaining (Supem atent)
1 5 . 0 0 -,
12.00 -
D)
9.00
6.00 -
01
3.00 .
0.00 -
0 05
2.19 I W
2.69
C2-
0.07
A 1 (Control)
3 5 .0 0
J 30.00 o 25.00
E 20.00
z 15.00
O 10.00
z 5.0 0
0.00
Nitrate (Supernatant)
------- e h s h s i-------1-------- P X K f& t-------H A 1 A 2 B1 B 2
File: A F F F Inhib (1CM2-97).x!s Sheet: N 0 3 - & N 0 2 Produced
B-106
US000011
IC C olum n C I' S tan d ard C urve
300
250
200
1 150 <
100
50
0
O .O O E +O O
2.00E + 09
4 .00E + 09
6.00E + 09
8.00E + 09
1.00E +10
C f C o n e, m g /l
IC Colum n N 0 2'- N S tan d ard Curve
m = 4.0OE-O8 i= 9.91E-01
m = 3.00E-08 i - -1.01E+0O
Phosphorus Standard Curve
m = ZOOE-08 ; = 8.12E-01
File: A F F F Inhib (17 -11-97).xls Sheet: Standards & Data
B-107
US00001 113
R e a c to r
AFFF (ppm )
A1 0
A2 0 A3 0 B1 60
B2 60 B3 60
H 202 m g/l
0
0 0
3000
3000 3000
Fe3+ m g/l
0
0
0
300
300
300
Stage Feedstock R R Decant E n d c< F e e d in g ~ E n d c< A n a e ro b ic "* E n d of Aerobic E rfended Aeration E n d of Settling E n d of Feeding** E n d ot A naerobic ** E n d o( Aerobic E n d at Settling E n d ot Feeding** E n d c4 A n a e ro b ic ** E n d of Aerobic E n d of Settling E nd of Feeding** E n d of A n a e ro b ic 1** E n d of Aerobic E rf ended Aeration E n d of Settling E n d of Feeding** E nd of A naerobic-* End of Aerobic E n d of Settling E n d of Feeding-- End of Anaerobic E n d of Aerobic E n d of Settling
STD 1 STD 2 STD 3 STD 4 STD 5 S T D 1--
STD 6 "
STD 7 "
Tim e
2 hr
4 hr
6 hr
(4hr)
8 hr 2 hr
4 hr
6 hr 8 hr 2 hr
4 hr
6 hr 8 hr 2 hr
4 hr
6 hr
(4hr)
8 hr 2 hr
4 hr
6 hr 8 hr 2 hr
4 hr
6 hr 8 hr
COD
ABS
(m gfl.)
% COD Removal
0 .3 8 0
0.021
9 73 .5 5 3 .0
0 .0 3 0
76.1
7 8 .9
0 .0 1 8
0.001 0.001
4 5 .3 13.3 1 3 .3
8 7 .4 96 .3 96 .3
0 002
2 6 .5
9 2 .6
0 .0 2 9
0.021
7 3 .5 5 3 .0
7 9 .6 8 5 .3
0 .0 0 3
3 9 .8
8 8 .9
0.002
2 6 .5
9 2 .6
0 .0 2 6
6 5 .8
8 1 .7
0 .0 2 3
58.1
8 3 .8
0.002
2 6 .5
9 2.6
0.001
13.3
9 6 .3
0.1 0 7
2 73 .5
6 7 .7
0 .0 9 5
0 010
2 4 2 .7 1 3 2 .7
7 1 .3 8 4 .3
0 .0 0 8 106.1
8 7 .5
0 .0 0 7
9 2 .9
8 9 .0
0 .1 1 3 0 .1 0 6
0.010
2 8 8 .9 2 70 .9 1 3 2 .7
65 9
68.0
8 4.3
0 .0 0 6
79.6
9 0 .6
0 .1 2 3 3 14 .5
6 2 .8
0 .0 1 6
0.012
2 12 .3 159 .2
7 4 .9 8 1 .2
0006
0.000
0089 0159
7 9 .6
9 0 .6
0 } Vials used range
1000 }fro m 100m g /l C O D
2000 ) T O 4 5 0 0 m g /l C O D
0 .2 1 8 0 .3 3 7
0.000
3000 4500
0
}
) } Viats used range
0 .1 9 6 0 .3 9 0
5 0 0 }from 20m g /l C O D
1000 } T O 9 0 0 m g /l C O D
F S (Filtered)** FS Average**
R R S U (Filtered)** R R SU A verage**
0 .3 8 0 0 .3 8 0
9 7 3 .5 973 .5
0.021 0.021
5 3 .0 5 3 .0
* The values of "CO D % Removal' shown in table and chart above are accumulative figures based on the initial COD concentration at time 0 hr.
** C O D measured with standard range vials (Range 20-900 mg/l)
File: AFFF Inhib (17-11-97).xls Sheet: CO D
B-108
US00001114
Initial COD a t Time 0 hr.
Sam ple C o n tro ls (A 1 .A 2& A 3)
C o n s titu e n t RR Decant F e e d s to c k AFFF
C O D m g /L
4 53 .0
2 9 73 .5 00
Total
2 1 1 .9 8 3 2 194 7 .0 1 5 4
0
3 5 9 .8
Inhibition (B 1.B 28B 3)
R R Decant Feedstock Fentons T reated A F F F
Total
4
2 2 6
53 .0 9 7 3 .5
1459
2 1 1 .9 8 3 2 1 9 4 7.01 54
2918 8 4 6 .2
COD Standard Curve for High Range Vials
m = 13266
/= 0
File: A FFF Inhib (17-11-97).xls Sheet: CO D
B-109
US00001115
COD Standard Curve for Standard Range Vials
Fentons Pretreatm ent For AFFF ppm
T a p w ater Actual A F F F ppm
H 202, 300C T2.5 m g/l Fe3+ 30C T2.5 F e $ 0 4 (3tim es)
60 R eacto r 1
1250 ml 150 5 ml
7 5 0 0 m g/l 7 5 0 m g/l 3 7 2 3 .2 8 m g/l
60 R eacto r 2
1250 ml 150 5 ml
7 5 0 0 m g/l 7 5 0 m g/l
3 7 2 3 .2 8 m g/l
R eacto r 3 1 2 5 0 ml 150 5 ml 7500m g/l 7 5 0 m g/l
3 7 2 3 .2 8 m g/l
Initial C O D A F FF-6 0 ppm
A bsorbance C O D m g/l
A ctual C O D
0 .1 3 1 1
1 7 3 7 .8
1 7 3 7 .8
CO D afte r 24 h r reactio n p erio d betw een fe n to n s reagent and A FFF
A b sorbance C O D m g/l A ctual C O D
0 .1 1 1
1 4 5 9 .3
145 9.3
% C O D Removal
16.0 3
File: A FFF Inhib (17-11-97).xls Sheet: COD
B-110
US00001116
Date of Test: 3-25-97
Oxygen Uptake Dissolved Oxygen
A FFF Cone. = 6 0 ppm
DA1 BA2 A 3 B1 DB2 B3
Oxygen Uptake curve during Aerobic stage
File: A F F F Inhib (17-11-97).xls Sheet: Oxygen Uptake
B-Lll
US00001117
S ta g e Feeding A n a e ro b ic A e ro b ic
Dissolved Oxygen (mg/L) at various stages
A1
0.11
0 .0 7 4 .6 8
A2 0 .09 0 .08 5 .30
A3 0 .0 9 0 .0 9 5 .0 7
81 0 .0 9 0 .0 9 5.21
B2 0 .0 8
0.10
5.27
B3
0.10
0 .0 9 5 .8 5
S ta g e A erobic
Tim e (sec)
0
30 60 90
120
150 180
210
240 270 300 330 360 390 420 450 460 510 540 570 j 600
A1 7 .8 4 5 .1 2 4.6 3 4.2 8 4 .0 1 3 .7 7 3 .5 4 3 .3 3 3 .1 2 294 2 .7 6 2 .58 2 43 2 .2 9 2 15
2.02
1.91 1 .7 9 1 .6 9 1.58 1.49
D is s o h e d O xygen in m g/L
A 2 A 3 B1
8 .8 3
8 .9 9
9.71
6 .9 4
6 .4 5
639
6.3 9
5 .8 0
5.61
6.0 3
5 .4 3
5 .2 2
574
5 .1 2
4 .8 9
548
4 .8 7
4 .5 9
526
4 65
4 .3 3
5 .0 5
4 .4 4
407
4 .8 4
4 .2 5
3.83
4.6 5 4 .46
4 .0 6 3 89
3.61 3 .4 0
429
3 .7 2
3.19
4 .1 1
3 .5 5
2.99
3 .9 5 3.8 0 3 .66 3 .5 2 3 .3 8 3 .2 5 3 .1 2 3.01
3 .3 9 3 24 3.11 2 .9 9
2.86
2.74 2.63 2.52
2.81 2.63 2 .4 6 2.29 2 .1 5
2.00 1 86
1.73
B2 9 .3 4 658 5 .6 8 5 .5 2 5.22 4,97 4 .7 4 4 .5 2 4 .3 0 4 .0 9 3 .9 0 3 .7 2 3 54 3 .3 7 3.21 306 2 .8 9 2 .7 5 2.61 2.48 2.36
B3 9 .4 7 6 .4 9 5 .6 9 5 .3 3 5 .0 6 4.81 4.5 9 4 .3 7 4 .1 7 3 .9 8 3 .7 9 3 61 3 .4 4 3 .2 8 3 .1 2 2 .9 6 2.81 2 .6 7 2 .5 4 2.41 2 .2 8
File: A F F F Inhib (17-t1-97).xls Sheet: Dissolved Oxygen
B-112
US00001118
Date of Test: 3-25-97
Concentrations Table
AFFF Cone. = 60 ppm
R e a cto r A1
A2
A3 B1
B2
B3
AFFF (ppm )
0
0
0 60
60 60
H202 m l
0
0
0 3000
3000 3000
Fe3*
m g/1
Stage
Feedstock
R R Decani
0 End of Feeding
E nd of Anaerobic
E n d of Aerobic
Extended Aeration
E n d o f Settling
0 End of Feeding
End of A naerobic
E nd of A erobic
End of Settling
0 E nd of Feeding
End of Anaerobic
End of A erobic
E n d of Settling
300
End of Feeding E nd of Anaerobic
End of Aerobic
Extended A eration
E n d of Settling
300
E nd of Feeding E nd of Anaerobic
E n d of Aerobic
E n d of Settling
3 0 0 E nd of Feeding
End of Anaerobic
E n d of Aerobic
E nd of Settling
C o n c e n tra tio n , m gfL
Time TKN NH3-N O n). N N 0 2 -N
N 03--N
Total N
Cl- P 0 4 -P
1 0 2 .9
20.7
8 2 .2
0 .0
0.8 1 0 3 .7 2 1 5 .9 1 1 .9
2.2 0 .4 1.8 - 0 .5 1 4 .8 1 6 .5 1 8 9 .3 2 .1
2 hr 2 8 .8
2 0 .7
8.1
0 .0
0.8 2 9 .6 2 0 1 .4 3 0 .3
4 hr 3 0 .5
19.6
10.9
0.0
0.8 3 1 .4 1 9 8 .8 3 2 .2
6 hr
6 .4
6 .1
02
3 .0 1 8 .4 2 7 .8 194.7 5 .2
(4 h f)
8 hr 2 hr
1 .5 0 .5 2 9 .5
0 .1
0.1
2 0 .7
1 .4 0 .4 8 .9
-0 .8 7 .2 0 .0
2 8.9 3 3.8
0.8
2 9 .6 4 1 .5 3 0 .4
1 9 3 .8
202.0
2 0 1 .4
6 .9
6.1 22.8
4 hr 2 7 .8
18.8
9 .0
0 .0
0.8 2 8 .7 1 9 7 .4 2 2 .5
6 hr
9 .6
6.6 2 .9
3.1
1 6 .0
2 8 .6 190.8 10.5
8 hr 2 hr
4 hr
6 hr
7.1 2 7 .8 2 7 .8
10.2
5 .3 19.9 19.6
6.6
1.8 8.0 8.2
3 .6
.9 17.1 2 5 .2 2 03 .7 11.7
0.0 0.8 2 8 .7 1 9 7 .9 15.1
oo 0.8 2 8 .7 2 0 3 .0 1 6 .2
3.1
1 5 .6
2 8 .9 1 8 8 0 8.4
8 hr
7.1
2 hr 19.7
4 hr 31,8
6 hr
(4hr)
137
31
8 hr
06
2 hr 2 7 .4
4 hr
6 hr 8 hr 2 hr
4 5 .6 108
8 .5 2 5 .9
4 hr 2 3 .0
6 hr
9 .2
I18 hr 92
49 16.3 1 5 .4
73
0.2 0.1
16.3 142
8 .4
6.0
16.3 15.4
8.8
65
2.2
3 .4 16.4
6 .3
2.8
0 .5
11.1
3 1 .4 2 .4 2 .5 9 .6 7 .6 0 .4 2 .7
0.8
0 .0 0 .0 3 .3
-0.2
0 .0 0 .0 0 .0 38 1 .7
00
-0 .4 3 .7 1 .4
1 6 .9
0.8 0.8
10.9 2 3 .2 37.4
0.8 0.8
9 .2 *
0.8
08 8 .7
2 4 .9 2 0 .5 3 2 .6
28 0
2 6 .0 3 8 .0 2 8 .2 4 6 .4 2 3 .9
102
26.7 2 3 .4
21.6
10.5
200.3 9 .6
195.4 16.9
200.0 1 9 .4
1 8 6 .4 12.1
1 8 9 .7 1 9 8 .9
11 2 12.2
196.3 12.7
201.2 1 7.4 187.4 24 8
202.2 2 4 .4
194.8 12.7
201.0 2 6 .5
184 9 19.747
198.5 10.682
* Values not known
File: A FFF Inhib (17-11-97).xls Sheet: Concentrations Table
B-113
U S 0 0 0 0 1 119
D ale of Test: 3-25-97
Concentrations Table
AFFF Cone. = 60 ppm
Reactor A1
A2 A3 B1
B2 B3
AFFF (ppm )
Stage
Tim e
F e e d s to c k
R R Decant
0 E n d of Feeding
2 hr
E n d of Anaerobic E n d of Aerobic
4 hr
6 hr
Extended Aeration (4hr)
E n d of Settling
8 hr
0 E n d of Feeding
2 hr
E n d of A naerobic E n d of Aerobic E n d of Settling
0 E n d of Feeding
4 hr
6 hr 8 hr 2 hr
E n d of Anaerobic E n d of Aerobic E n d of Settling 6 0 E n d of Feeding
4 hr
6 hr 8 hr 2 hr
E n d of Anaerobic E n d of A erobic
4 hr
6 hr
E x te n d e d Aeration <4hr)
E n d of Settling
8 hr
6 0 E n d of Feeding
2 hr
E n d of Anaerobic E n d of A erobic E n d of Settling 6 0 E n d of Feeding
4 hr
6 hr 8 hr 2 hr
E nd of Anaerobic E nd of A erobic E n d of Settling
4 hr
6 hr 8 hr
FS1
FS2
FS3
FS Avarage
RRSU1
RRSU2
RRSU3
R R S U Avarage
C o n c e n tra tio n , m g/L
BOO
COD
TOC
9 7 3 .5 5 3 .0
406 6 12.1
76.1
3 0 .7
4 5 .3
1 9 .5
13.3
1 4 .8
13.3
14.0
2 6 .5
13.4
7 3 .5
28.6
5 3 .0
1 7 .5
3 9 .8
12.4
2 6 .5
65 8
13.7 2 8 .4
58.1
18.2
2 6 .5 13.3
12.7
12.6
2 7 3 .5
8 5 .2
2 4 2 .7
76.1 '
1 3 2 .7
5 8 .9
106.1
5 8 .0
9 2 .9
6 0 .0
2 8 8 .9
83.1
2 7 0 .9
76.1
1 3 2 .7
59.4
79.6
59.8
3 1 4 .5 .
85.8
2123
77.7
73 61.84
<5 62.23
404 .6
4 07 .5
4 07 .7
4 06 .6
12.9
11.3
12.0
1 2 .0 8
| Alkalinity H C 0 3 (mg/T) 4 4 7 .0 309 .0 498 .0 523 .0 331 .0 2 6 3 .0 2 62 .0 514 .0 480 0 337 .0 327 .0 4 8 0 .0 500 .0 3280 331 0 563 .0 534 0 411 .0 2 74 .0 3 28 .0 5 90 .0 546 0 414 0 428 .0 553 0 5230 412 437
File: A FFF Inhib (17-11-97).xls Sheet: Concentrations Table
B-114
US00001120
Date of Test: 3-25-97
NH3-N & TKN Charts
A FFF Cone. = 60 ppm
Inhibition Effect o f AFFF on Nitrification in terms of NH3-N
-- -- A1 -- -- A2 --A-- A3 -- -- B1 -- X -- B2
B3
Inhibition Effect o f A FFF on Nitrification as o f N 3-N
/
Tim e
Inhibition effect of AFFF on Nitrification as of TKN
-- A1
A2
--A -- A3
-- -- B1
-- B2
--5 3 -B 3
SFihlee:eAt: FNFHF3I-nNhi&b T(1K7N-1C1-h9a7rJt.sxls
B-115
1
US00001121
Date of Test: 3-25-97
NH3-N & TKN Charts
Inhibition effect of AFFF on Phosphorus removal
AFFF Cone. = 60 ppm
Senesi S e rie s 2 S eries3 S eries4 S eries5
Series6
Rie: A FFF Inhib (17-11-97).x!s Sheet: NH3-N & TKN Charts
B-116
I
i
US00001122
Date of Test: 3-25-97
Inhibition Reactors / Nitrogen Mass Balance
A FFF Cone. = 6 0 ppm
File: A FFF Inhib (17-11-97J.xls Sheet: Nitrogen Mass Balance
B-117
US00001123
Date of Test: 3-25-97
Inhibition Reactors / N 0 3 - & N 0 2 Produced
AFFF Cone. 60 ppm
B -1 1 8
i
US00001124
BNR In h ibition T ests - 60 ppm A FFF Pretreated w ith Fenton's Reagent (Nov/17/97)
TSS Reactor Initial wt
A1 1.114 A2 . 1.1126 A3 1.1199 B1 1.1154 B2 1.1113 B3 1.0983
Final wt 1.1559
- 1.156 1.162 1.1586 1.1527 1.1394
Volum e 15 15 15 15 15 15
MLSS 2793 2893 2807 2880 2760 2740
WT @ 550 C MLVSS
1.1187
2480
1.1191
2460
1.1255
2433
1.1154
2880
1.1172
2367
1.1025
2460
TDS Reactor A1 A2 A3 B1 B2 B3
Initial wt 1.0134 1.0147 1.0153 1.0436 1.0465 1.0307
Final wt 1.0261 1.0293 1.0303 1.0945 1.0952 1.0745
Volum e 15 15 15 15 15 15
TDS 847 973 1000 3393 3247 2920
TS Reactor A1 A2 A3 B1 B2 B3
Initial wt 1.0312 1.0166 1.0122 1.0083 1.0141 1.0109
Final wt 1.13
1.0746 1.0704 1.1003 1.1064 1.1044
V o lu m e 25 15 15 15 15 15
TS 3952 3867 3880 6133 6153 6233
ETSS.TDS 3640 3867 3807 6273 6007 5660
File: A FFF Soilds Data
B-119
US00001125
APPENDIX C BNR Inhibition Batch A ssays Pretreated with Fenton's Reagent
c-i
US00001126
U S 0 0 0 0 1 127
BNR Inhibition Batch A ssay Pretreated with Fenton's Reagent (AFFF 60 ppm)
C-3
US00001128
R e a c to r
AFFF (ppm )
HA, m grt
A1 0 0
A2 0
0
A3 0
0
B1 60 3000
B2 60 3000 B3 60 3000
F e 5* m g/l
0
0 0
300
300 300
S ta g e F e e d s to c k R R Decant E n d o f F ee d in g E n d of A n aerobic E n d o f A ero bic E x te n d e d A eration E n d o f S ettling E n d o f F ee d in g E n d of A n aerobic E n d o f A e ro b ic E n d o f S ettling E n d o f F ee d in g E n d of A n aerobic E n d o f A erobic E n d o f S ettling E n d of Fee d in g E n d of A n aerobic E n d of A ero bic E x te n d e d A eration E n d of S ettling End of F ee d in g End of A n aerobic E n d of A ero bic E n d of S ettling E nd of F eeding End of A n aerobic End of A e ro b ic End of S ettling
Tim e
2 hr
4 hr
6 hr
(4 h r)
8 hr 2 hr
4 hr
. 6 hr 8 hr 2 hr
4 hr
6 hr 8 hr 2 hr
4 hr
6 hr
(4 h r)
8 hr 2 hr
4 hr
6 hr 8 hr 2 hr
4 hr
6 hr 8 hr
N H 3-N (m gfl.)
20.7 0.4
20.7 19.6
S.1
0.1 0.1
20 .7 18 .8
6.6
5 .3 1 9.9 1 9 .6
6.6
4 .9
1 6 .3 1 5 .4
7 .5
0.2 0.1
1 6 .3 14 .2
8 .4
6.0
1 6 .3 1 5 .4
8.8
6 .5
TKN
(m g/L)
102. ' 2.2>
28.8
4 4 7 .0 309 0 4 9 8 .0
3 0 .5
5 2 3 .0
6 .4 1 .5 0 .5 2 9 .5 2 7 .8
3 3 1 .0 2 6 3 .0
262 0
5 1 4 .0 4 8 0 .0
9 .6 7 .1 2 7 .8
3 3 7 .0 327 0 4 8 0 .0
2 7.8
10.2
7 .1
5 0 0 .0 3 2 8 .0 3 3 1 .0
1 9 .7 31 .8 1 3 .7
5 6 3 .0 5 3 4 .0 4 1 1 .0
3.1
0.6
2 7 .4
2 7 4 .0 328 0 5 9 0 .0
4 5.6
10.8
B.5 2 5.9 2 3.0
5 4 6 .0 4 1 4 .0 4 2 8 .0 5 5 3 .0 5 2 3 .0
9.2 4 1 2 .0
9.2 4 3 7 .0
File: AFFF Inhib (17-11-97).xls Sheet: Standards & Data
C-4
US00001129
A FFF R ea cto r (ppm )
mg/1
A1 0 0
A2 0 0 A3 0 0 B1 60 3000
62 60 3000 B3 60 3000
--------f-
F e 3* mg/1
S ta g e
F e e d sto ck R R Decani 0 End of Feeding
End of A naerobic End of A erob ic Extended A eration
End of Settling 0 End of Feeding
- End of A naerobic End of A erob ic End of Settling
0 End of Feeding
End of A naerobic
End of A erob ic E n d o f Settling 300 End of Feeding End of Anaerobic End o f A erob ic
Extended A eration End o f Settling 300 End of Feeding
End of Anaerobic E n d of A erobic E n d of Settling 300 End of Feeding End of A naerobic End of A erob ic :n d of Settling
stand 2 Stand 3
Tim e
Ct
A rea
(m g/L
N O -N
A rea
(m g/L
N O j-N A rea
580773486? 21 5 .9
0
0 .0
0
2 hr 4 hr 6 hr (4 h r) 8 hr 2 hr 4 hr
508B03005S 5414358836 5345974926 5235496411 5210918794 5432080598 5416022378 5307025713
189 3 2 0 1 .4
18533649 0
1 9 8 .8 1 9 4 .7 1 9 3 .8
0 160999406 10376403
2 0 2 .0 2 0 1 .4 1 9 7 .4
327233852 0 0
- 0 .5 0 .0 0 .0 30 -0 .8 7 .2 0 .0 0 .0
865888608 84908 212574
1084830545 1734473723 2039121450
89976 52180
6 hr 5130364031 190.8 162417541
3 .1
935491198
8 hr 54767 73 6 8 2 203.7 76258681
0.9 1007957588
2 h r 5 321776591 197.9
0
0.0 217 9 2 0
4 hr 5457976525 203.0 40 7 1 0 49 0
0 .0
1763367
6 hr 8 hr
5053601257 5387232572
1 8 8 .0 2 0 0 .3
165207674 71938053
3 .1 0 .8
913754761 995657727
2 h r 5254 5 03 7 1 0 195.4
0
0 .0
0
4 hr 53778 09 7 7 5 200.0
0
0 .0 100640
6 hr 50095 30 8 7 5 186.4 172102121 3 3 6 2 3 8 9 0 4 1 4
(4hr> 5 0 9 8 9 7 9 3 7 4
8 hr 5 3 4 7 9 72 5 7 0
2 hr 5276618823
4 hr 5410720650
6 hr 8 hr
5037470503 5438217127
1 8 9 .7 1 9 8 .9 1 9 6 .3 201 2 1 8 7 .4 2 0 2 .2
31000471 0 0 0
192838355 108285687
-0 .2 0 .0 0 .0 00 38 1 .7
1380576510 2257465140
0 0 519045648 -
2 hr 4 hr 6 hr 8 hr
5237933600 5406092060 4969066373 5337021830
1 9 4 .8 2 0 1 .0 1 8 4 .9 1 9 8 .5
0 22624100 188964308 94581465
0 .0 -0 .4 3 .7 1 .4
0 0 489642483
2587238260 5219091409
9 8 .7 3 1 9 4 .1
444494781 917655285
1 0 .1 4 2 2 .0
529102517 1148516754
(m g/L) 0 .8 1 4 .8 0 .8 0 .8 1 8 .4
2 8 .9 3 3 .8 0 .8 0 .8 1 6 .0 17.1 0 .8 0 .8 15 .6 1 6 .9 0 .8 0 .8 10 .9 2 3 .2 37 .4 0 .8 08 92 *
08 0 .8 87
-
9 .3 8 1 9 .4
P C 4 -P
A b so rb mg/I
0 378 0 .0 4 9 0 .9 9 5
1 1 .9 3 ? 1? 3 0 .3 3
1 .0 5 9
3 2 .2 4
0 .1 5 3 0 .2 0 8
522 6 .8 7
0 .1 8 3 0 .7 4 4 0 .7 3 4
6 12 2 2 .8 5 2 2 .5 5
0 .3 3 0 .3 7 1 0 .4 8 3
1 0 .5 0 11 73 1 5 .0 7
0 .5 2 2 0 .2 5 8 0 301
16 23 8 .3 6 9 .6 4
0 .5 4 4 0 .6 7 7 0 383
1 6 .8 8 1 9 .3 6 1 2 .0 8
0 353 0 387 0 .4 0 4
1 1 .1 9 1? 20 1 2 .7 1
0 .5 6 0811 0 .7 9 7 0 .4 0 4 0 .8 6 7 0 64
1 7 .3 6 2 4 .8 5 24 43 12.71 2 6 .5 2 1 9 .7 5
0 .3 3 6
1 0 .6 8
iS t a n d a r d s u s e d
STD 1 STD 2 STD 3 STD 4
0 2594976044 5464371291 8047270041
00 100 444761304 200 925169445 300 1.582E+ 09
00 10 531020858 20 1144250131 40 7447042210
0 0.158 10 0.636 20 1.14 40 1.666
5 20 35 50
* -- Value not known
R e: AFFF Inhib (17-11-97).xls Sheet: Standards & Data
C-5
US00001130
IC C olum n C I' S tandard C urve
CP C o n e , m g /l
1C Column N 0 2 - N Standard Curve
m = 4.00E -08 ;' = 9 .9 1 E -0 1
m = 3.00E -08 i= -1.01E + 00
1C C o lu m n N O j'- N S ta n d a r d C u rv e
m = 2.00E -08 / = 8.12E-01
File: AFFF Inhib (17-11-97).xls Sheet: Standards & Data
C-6
US00001131
SUMMARY O UTPUT
CL
R eg ression Statistics
R Square
0 .9 9 9 5 6 7 9 6
O bservatio n s
4
In te rc e p t X V ariable 1
Coefficients S tandard Error 0.9 9 0 9 2 7 3 4 2 .7 3 8 3 9 1 3 7 3 .7 0 0 6 E -0 8 5.4401 E -10
SUMMARY O UTPUT
N 0 2 -N
R eg ression Statistics
R Square O bservatio n s
0 .9 9 1 2 9 2 6 8 4
In te rc e p t X V a riab le 1
Coefficients Standard Error -1 .0 1 4 3 1 7 9 6 1.5 6 7 7 4 3 3 1
2.5 0 8 6 E -0 8 1.6 6 2 5 E -0 9
SUMMARY O UTPUT
N 0 3 -N
R egression Statistics
R Square
0 .9 9 8 1 3 4 9 8
O bservatio ns
4
Coefficients S tandard E rro r
in te rc e p t
0 .81231 028 0 .6 8129 031
X V a riab le 1
1 .6 1 9 3 E -0 8 4 .9 4 9 3 E -1 0
SUMMARY O UTPUT
P 0 4 -P
R eg ression Statistics
R Square O bservatio ns
In te rc e p t X V a riab le 1
0 .9 9954 389 4
Coefficients Standard E rror 0 .6 6 2 6 0 4 3 0 .4 7 7 9 8 4 8 2
2 9 .8 1 9 3 2 8 6 0 .4 5 0 4 1 7 4 6
File: A F F F Inhib (17-11-97).xls Sheet: Standards & Data
C -7
US00001132
R eactor
AFFF (ppm )
H 202
m g/l
. A1
0
0
A2 0
0
A3 0
0
B1 60 3 0 0 0
B2 60 3000 B3 60 3000
Fe3+ m g /l
0
0 0
300
300 300
S ta g e F e e d s to c k R R Decant E n d of F eeding ** E n d of A n aerobic** E n d of A erobic E xten ded A eration E n d of Settfing E n d of Fee d in g ** E n d of A n aerobic ** E n d of A erobic E n d of S ettling E n d of F eeding ** E n d of A naerobic** E n d of A erobic E n d of S ettling E n d of Fee d in g ** E n d of A naerobic** E n d of A erobic E xtended A eration E n d of Settling E n d of F ee d in g ** E n d of A naerobic** E n d of A ero bic E n d of S ettling E n d of F eeding ** E n d of A n aerobic E n d of A erobic E n d of S ettling
STD 1 STD 2 STD 3 STD 4 STD 5 STD 1"
STD 6 "
STD 7 **
Tim e
2 hr
4 hr
6 hr
(4 h r)
8 hr 2 hr
4 hr
6 hr 8 hr 2 hr
4 hr
6 hr 8 hr 2 hr
4 hr
6 hr
(4 h r)
8 hr 2 hr
4 hr
6 hr 8 hr 2 hr
4 hr
6 hr 8 hr
COD
ABS
(m g/L)
0 .3 8 0
0.021
9 7 3 .5 53 .0
0 .0 3 0
7 6 .1
0 .0 1 8
0.001 0.001 0.002
45 .3 1 3 .3 1 3.3 26 .5
0 .0 2 9
0.021
73 .5 53 .0
0 .0 0 3
39 .8
0.002
2 6 .5
% COD Removal
78 .9 87 .4 96 .3 96 .3 92 .6 79 .6 8 5 .3 8 8 .9 9 2.6
0 .0 2 6
6 5.8
8 1 .7
0 .0 2 3
0.002 0.001
5 8 .1 2 6 .5 1 3.3
8 3 .8 9 2 .6 96 .3
0 .1 0 7
2 7 3 .5
67 .7
0 .0 9 5
0.010
2 4 2 .7 1 3 2 .7
71 3 8 4.3
0 .0 0 8 0 .007 0 .1 1 3 0 .1 0 6
0.010
0 .006 0 .1 2 3 0 .0 1 6
0.012
0 .0 0 6 0 .0 0 0 0 .0 8 9 0 .1 5 9 0 .2 1 8 0 .3 3 7
0.000
0 .1 9 6 0 .3 9 0
1 0 6 .1
8 7 .5
92 .9 2 8 8 .9 2 7 0 .9
89 .0 65 .9
68.0
132 .7
8 4.3
7 9 .6
9 0.6
3 1 4 .5
6 2.8
2 1 2 .3
74 .9
1592
8 1 .2
79 .6
9 0 .6
0 } V ials used range
1000 )frc m 1 0 0 m g /IC O D
2000 ) T O 4 5 0 0 m g /l C O D
3000 4500
0
} } } V ials used ra n g e '
500
1000
Jfrom 20m g /t C O D } T O 9 0 0 m g /l C O D
F S (F iltered )** FS A verage**
0 .3 8 0 0 .3 8 0
9 7 3 .5 9 7 3 .5
R R S U (F itte re d )** R R SU A verage**
0.021 0.021
5 3 .0 5 3 .0
* The v alu es of "COD % R em o v al" shown In table and chart ab o v e are accum ulative figures b ased on (he initial COD concentration at iime G hr.
** COD m e a su re d with stan d ard range vials.(R ange 20-SG0 mg/l)
File: A F F F Inhib (17-11-97).xls Sheet: C O D
C-8
i
US00001133
S a m p le C ontrols (A 1 A 2 & A 3 )
Initial CO D at Tim e 0 hr.
C onstituent R R Decant F e e d s to c k AFFF T ria l
V o l (L)
0
C O D m q /L 5 3 .0
9 7 3 .5
0
2 1 1 .9 8 3 2 1 9 4 7 .0 1 5 4
o 3 5 9 .8
In h ib itio n (B 1 .B 2 S B 3 )
R R Decant F e e d s to c k Fen ton s T reated A F F F
2
5 3 .0 9 7 3 .5 1459
2 1 1 .9 8 3 2 1 9 4 7 .0 1 5 4
2918
COD Standard Curve fo r High Range V ials
m = 13266
/= 0
File: A F F F Inhib (17-11-97).xls Sheet: C O D
C-9
US00001134
CO D Standard Curve for Standard R ange Vials
I Fentons P retreatm ent
For AFFF ppm
60
60
60
_________________ R e a c t o r 1
T a p w ater
1250 ml
A ctual A F F F p p m
150
H 2 0 2 , 3 0 0 0 *2 .5 m g/l
5 ml 7 5 0 0 m g/!
Fe3+ 3 0 0 *2 .5 F e S 0 4 (3!im es)
7 5 0 m g/l 3 7 2 3 .2 8 m g/l
R e a c to r 2 1250 ml 150 5 ml
7 5 0 0 m g/l 7 5 0 m g/! 3 7 2 3 .2 8 m g/l
R e a c to r 3 1250 ml 150 5 ml
7 5 0 0 m g /l 7 5 0 m g/l 3 7 2 3 .2 8 m g/l
In itia l C O D A F F F -6 0 ppm
A bsorbance
C O D m g/l
A ctu al C O D
0 .1 3 1 1
1 7 3 7 .8
1 7 3 7 .8
C O D a fte r 24 h r re a c tio n p e rio d b e tw e e n te n to n s re a g e n t a n d A F F F
% C O D Removal
A b s o rb a n c e C O D m g/l
A ctu al C O D
0 .1 1 1
1 4 5 9 .3
1 4 5 9 .3
1 6 .0 3
File: A FFF Inhib (17-11-97).xls Sheet: C O D
C-10
US00001135
S tage F ee d in g A n aerobic A e ro b ic
D issolved O xygen (mg/L) at various stag es
A1 A 2 A 3 B 1 B2
0.11
0 .0 9
0.0 9
0 .09
0 .0 8
0.0 7
0 .0 8
0.0 9
0 .09
0.10
4.6 8
5 .3 0
5.0 7
5 .2 1
5 .27
B3
0.10
0 .0 9 5 .8 5
S tage A e ro b ic
T im e (sec)
0
30 60 90
120
150 180
210
240 270 300 330 360 390 420 450 480 510 540 570 600
A1 784 5 .1 2 463 4.2 8 401 3 .7 7 3 .5 4 3 .33 3 .12 2 .9 4 276 2 58 243 2 29 2 .1 5
2.02
1 91 1 .7 9 1 .6 9 1 58 1.491
D isso lved O x y g e n in m g/L
A2 8 .8 3
A3 8 .9 9
B1 9.71
6 .9 4
6 .4 5
6 .39
6 .3 9
5.8 0
5 .6 1
6 .0 3
5 .4 3
5 .22
5 .7 4
5.1 2
4.8 9
5 .4 8
4.8 7
4.5 9
5 .2 6
4 .6 5
4.3 3
5 .0 5
4.4 4
4 .07
4 .8 4
4.2 5
3.83 '
4 .6 5
4.0 6
3 .6 1
4 .4 6
3 .8 9
3 .40
429
3 .72
3 .19
4 .11
3.5 5
2.9 9
3 .9 5
3 39
2 .8 1
3 .8 0
3 .2 4
2.6 3
3 .6 6
3 .1 1
2.4 6
3 .5 2 3 .3 8 3 .2 5 3 .1 2
2.9 9
2.86
2.7 4 2.6 3
2.2 9 2.1 5
2.00 1.86
3 .0 1 1
2.5 2
1 .7 3
B2 9 .34 6 .5 8 5 .8 8 5 .5 2 5.2 2 4 .9 7 4 .7 4 4 .5 2 4 .3 0 4 .0 9 3 .9 0 3 .7 2 3.5 4 3.3 7 3 21 3.0 6 2 .8 9 2 .7 5 2 .6 1 2 .4 8 2 .3 6
B3 9 .4 7 6 .4 9 5 .6 9 5 .3 3 5 .0 6 4 .8 1 4 .5 9 4 .3 7 4 .1 7 3 .9 8 3 .7 9 3 .61 3 .4 4 3 .2 8 3 .1 2 2 .9 6 2.8 1 2 .6 7 2 .5 4 2 .4 1 2 .2 8
File: AFFF Inhib (17-11-97).xls Sheet: Dissolved Oxygen
C -ll
US00001136
Date of Test: 3-25-97
Oxygen Uptake
Dissolved Oxygen
AFFF Cone. = 60 ppm
DAI A 2
DA3
DB1
DB2
DB3
O xygen U ptake curve during A erobic stage
File: A FFF Inhib (17-11-97).xls Sheet: Oxygen Uptake
C-12
US00001137
Dale of Tesi: 3-25-97
Concentrations Table
AFFF Cone. = 60 ppm
R eacto r A1
A2 A3 B1
B2 ' B3
AFFF H 202 (ppm ) m g/l
00
00 00 60 3000
60 3000 60 3000
Fe3+
Concentration, m g /L
m g/l
S ta g e
T im e T K N N H 3 -N O rg . N N 0 2 --N N 0 3 - - N T o t a l N
F e e d s to c k
1 0 2 .9
20 .7
8 2 .2
0.0
0.8 1 0 3 .7
R R Decant
2.2 0 .4 1.8 - 0 . 5 1 4 . 8 1 6 . 5
0 E n d of F ee d in g
2 hr 2 8 .8
20 .7
8.1
0 .0
0.8 2 9 .6
E n d of A n aerobic
4 hr 3 0 .5
1 9.6
10 .9
0 .0
0.8 3 1 .4
E n d of A e ro b ic
6 hr
6 .4
6.1 0.2
3 .0 1 8 .4 2 7 .8
E xten ded A eration (4h r)
1 .5
0.1 1 .4
- 0.8
2 8 .9
2 9 .6
E n d o f S e ttling
8 hr
0 .5
0.1 0 .4
7 .2 3 3 .8 4 1 .5
0 E n d of F e e d in g
2 hr 2 9 .5
20 .7
8 .9
0.0
0.8 3 0 .4
E n d of A n aerobic
4 hr 2 7 8.
188
90
0 .0
0.8 2 8 .7
E n d of A e ro b ic
6 hr
9 .6
6.6 2 .9
3 .1
16 .0
2 8 .6
E n d o f S e ttlin g
8 hr
7 .1
5 .3 1.8
0 .9 17.1 2 5 .2
0 E n d of F e e d in g
2 hr 2 7 .8
1 9 .9
8.0
0.0
0.8 2 8 .7
E n d of A n aerobic
4 hr 2 7 .8
1 9 .6
8.2
0.0
0.8 2 8 .7
E n d of A e ro b ic
6 hr 10.2 6.6 3 .6
3 .1
15 .6
2 8 .9
E n d of S e ttling
8 hr
7 .1
4 .9 2.2
0.8 1 6 .9 2 4 .9
3 0 0 E n d of Fee d in g
2 hr
19 .7
1 6 .3
3 .4
0 .0
0.8 2 0 . 5
E n d of A n aerobic
4 hr 3 1 .8
1 5 .4
1 6 .4
0 .0
0.8 3 2 .6
E n d of A e ro b ic
6 hr
1 3 .7
7 .5 6 .3
3 .3 1 0 .9 2 8 .0
E x ten d ed A eration (4hr)
3 .1
0.2 2.8
-0.2
2 3 .2
2 6 .0
E n d of S ettling
8 hr 0.6 0 1 0 .5
0 .0 3 7 .4 3 8 .0
3 0 0 E n d of Fee d in g
2 hr 2 7 .4
1 6.3
11.1
0 .0
0.8 2 8 .2
E n d of A naerobic
4 hr 4 5 6
14 2
31 .4
0 .0
0.8 4 6 .4
E n d of A e ro b ic
6 hr
108
8 .4 2 .4
3 .8
9 .2 2 3 .9
E n d of S ettling
8 hr
8 .5
6.0 2 .5
1 .7
* 10.2
3 0 0 E n d of Fee d in g
2 hr 2 5 .9
163
9 .6
0.0
0.8 2 6 .7
E n d of A n aerobic
4 hr 2 3 .0
1 5 .4
7 .6
-0 .4
0 8 2 3 .4
E n d o f A e ro b ic
6 hr
92
88 0 4
3 .7
8 .7 21.6
!E n d of S ettling
| 8 hr
9 .2
6 5 2.7
1 .4
* 10 .5
C l- P 0 4 - P
2 1 5 .9 1 8 9 .3
11 .9
2.1
2 0 1 .4 3 0 .3
1 9 8 .8 3 2 .2
194 .7
193 8 202.0
5 .2 6 .9
6.1
2 0 1 .4 22.8
197 .4 2 Z 5
1 9 0 .8 1 0.5
2 0 3 .7 1 1 .7
1 9 7 .9 2 0 3 .0
1 5 .1 16 .2
1 8 8 .0 8 .4
2 0 0 .3 9 .6
195 .4
200.0
1 86 .4 189 .7 1 9 8 .9
16 .9 1 9 .4
12.1 11.2 12.2
1 9 6 .3
201.2
187 .4
202.2 194 8 201.0
1 2 .7 1 7.4 2 4 .8 24 .4 1 2.7 2 6 .5
1 8 4 .9 19.747
1 9 8 .5 1 0 .6 8 2
* V alues not known
File: AFFF Inhib (17-11-97).xls Sheet: Concentrations Table
C-13
US00001138
Dale of Test: 3-25-97
Concentrations Table
AFFF Cone. = 60 ppm
R eactor A1
A2 A3 B1
B2 B3
AFFF
(ppm )
S ta g e
F e e d s to c k
Tim e
R R Decant
0 E n d of F eeding
2 hr
E n d of A n aerobic
4 hr
E n d o f A erobic
6 hr
E xten ded A eration (4 h r)
E n d of S ettling
8 hr
0 E n d of F eeding
2 hr
E n d of A n aerobic
4 hr
E n d of A ero bic
6 hr
E n d o f S ettling
0 E n d of F eeding
8 hr 2 hr
E n d of A n aerobic E n d of A ero bic E n d of S ettling 6 0 E n d of F ee d in g
4 hr
6 hr 8 hr 2 hr
E n d of A n aerobic E n d of A erobic
4 hr
6 hr
E xten ded A eration (4 h r)
E n d of S ettling
8 hr
6 0 E n d of F ee d in g
2 hr
E n d of A n aerobic E n d of A ero bic E n d of S ettling 6 0 E n d of F eeding
4 hr
6 hr 8 hr 2 hr
E n d of A n aerobic E n d of A erobic E n d of S ettling
4 hr
6 hr 8 hr
FS1
FS2 FS3
F S Avarage
RRSU1
RRSU2
RRSU3
R R S U Avarage
C o n c e n tra tio n , m g /L
BOD
COD 9 7 3 .5
5 3 .0
TOC
406 6 12.1
76 .1
30 .7
4 5 .3
1 9 .5
13 .3 13 .3
14 .8 14 .0
2 6 .5
1 3.4
7 3 .5 5 3 .0
2 8 .6 1 7 .5
3 9 .8
12 .4
2 6 .5
1 3.7
6 5 .8
28 .4
5 8 .1
18 .2
2 6 .5 13 .3
12 .7
12.6
2 7 3 .5
8 5 .2
2 4 2 .7
7 6 .1
1 3 2 .7
5 8.9
1 06 .1
5 8 .0
9 2 .9
6 0 .0
288 9
8 3 .1
270 9 132 .7
7 6 .1 59 .4
7 9 .6 3 1 4 .5
5 9 .8 85 .8
2 1 2 .3 73 <5
77 .7 61 84 6 2 .2 3
4 0 4 .6
4 0 7 .5 4 0 7 .7
406 6
12 .9
11 .3
12.0
1 2 .0 8
| A lkalin ity H C 0 3 (m g /l) 4 4 7 .0 3 0 9 .0 4 9 8 .0 5 2 3 .0 3 3 1 .0 2 6 3 .0 2 6 2 .0 5 1 4 .0 4 8 0 .0 3 3 7 .0 3 2 7 .0 4 8 0 .0 5 0 0 .0 3 2 8 .0 3 3 1 .0 5 6 3 .0 5 3 4 .0 4 1 1 .0 2 7 4 .0 3 2 8 .0 5 9 0 .0 5 4 6 .0 4 1 4 .0 4 2 8 .0 553 0 523 0 412 437
File: A F F F Inhib (1 7 -1 1-97).xls Sheet: Concentrations Table
C-14
US00001139
Date of Test: 3-25-97
NH3-N & TKN Charts
AFFF Conc. = 60 ppm
Inhibition Effect of A FFF on N itrification in term s of N H 3-N
-- *-- Al --a -- A2 -- A-- A3
-- B1
-- -- B2 -- -- B3
Inhibition Effect o f AFFF o n N itrification as of NQ 3-N
1 /
Inhibition effect o f AFFF on N itrification as o f TK N
s i
File: A FFF Inhib (17-11-97).xls Sheet: N H 3-N & TKN Charts
C-15
US00001140
Date of Test: 3-25-97
NH3-N & TKN Charts
Inhibition effect of A FFF on Phosphorus rem oval
AFFF Cone. = 60 ppm
I
File: AFFF Inhib (17-11-97).xls Sheet: NH3-N & TKN Charts
C-16
US00001141
Date of Test: 3-25-97
Inhibition Reactors / Nitrogen Mass Balance
AFFF Cone. = 60 ppm
Reactor A1 (No AFFF present)
End of F e e d in g
77777,
lilil
End of
End of
A n aero b ic
A e ro b ic
S tag e
w
End of S ettling
D N 0 3 -N E 3 N 02-N B O rg -N N H 3-N
Reactor A 2 (No AFFF present)
. 35.0 -p
- 30.0 f 25.0 -
c 20.0 -
a>
o> 1 50 -
1 10.0 -
2 50
0.0 - -
E nd of Fee d in g
End of A n aerobic
E nd of A e ro b ic
End of S e ttling
S tag e
N 0 3 -N N 0 2 -N aO rg . N N H 3-N
Reactor A 3 (No AFFF present)
N 0 3 -N N 0 2 -N B O rg. N E JN H 3-N
End of F e e d in g
End of
End of
A n aero b ic
A e ro b ic
S tag e
End of S ettling
File: A F F F Inhib (17-11-97).xls Sheet: Nitrogen Mass Balance
C -1 7
US00001142
Dale of Test: 3-25-97
Inhibition Reactors / Nitrogen Mass Balance
AFFF Cone. = 60 ppm
File: A F F F Inhib (1 7 -1 1-97).x!s Sheet: Nitrogen M ass Balance
C-18
US00001143
Date of Test: 3-25-97
Inhibition Reactors / N03- & N02 Produced
AFFF Cone. = 60 ppm
R e acto r A1 (C ontrol)
A FFF ppm 0
H 202 m g /l 0
m g l 0
A2 (C ontrol)
0
A3 (C ontrol)
0
B1 6 0
00
00
3000
300
B2 60 B3 60
3000
300
3000
300
Fe3+ S tag e E n d of Feeding E n d of Anaerobic E n d of A ero bic E x te n d e d A eration E n d of Settling E n d of Feeding E n d of Anaerobic E n d of A erobic E n d of Settling E n d of Feeding E n d of Anaerobic E n d of A erobic E n d of Settling E n d of Feeding E n d of A n aerobic E n d o f A erobic E x te n d e d Aeration E n d of Settling E n d of Feeding E n d of A naerobic E n d of A erobic E n d of Settling E n d of Feeding E n d of A naerobic E n d of A ero bic E n d of S ettling
n itro g e n C o n c e n tra tio n , m g /L
Tim e
N H 3-M N 0 3 -N
N 0 2 -N
A ( N 0 3' - N )
2 hr
2 0 .6 9
0 .8
0 .0
4 hr
1 9 .6 1
0 .8
0 .0
6 hr
6.1 1
1 8 .4
3 .0
(4 h r)
0 .1 1
2 8 .9
-0 .8
8 hr
0 .1 1
3 3 .8
7 .2 3 3 .0 2
2 hr
20 69
0 .8
0 .0
4 hr
1 8 .8 4
0 .8
0 .0
6 hr
6 .6 2
16 .0
31
8 hr
5 .3 1
17.1 .
09
1 6 .3 2
2 hr
1 9 .8 8
0 .8
0 .0
4 hr
1 9 .6 1
0 .8
0 .0
6 hr
6 .6 2
1 5.6
3 .1
8 hr
4 .9 0
1 6 .9
0 .8 1 6 .1 2
2 hr
1 6 .2 6
0 .8
0 .0
4 hr
15.4 2
0 .8
0 .0
6 hr
7 .4 6
1 0 .9
3 .3
(4 h r)
0 .2 3
23 .2
-0 .2
8 hr
0 .1 4
3 7 .4
0 .0 3 6 .5 5
2 hr
1626
0 .8
0 .0
4 hr
1 4.23
0 .8
0 .0
6 hr
8 .4 2
9 .2
3 .8
8 hr
5 99
- 1.7 8 .4 0
2 hr
1 6 .26
0 .8
00
4 hr 1 5 .4 2 0 8 - 0 4
6 hr 8 hr
876 6 .4 9
8 .7 4 *
3 .7 3 1 .3 6
7 .9 3
|g-| I
A m m onia Rem aining (Supernatent)
Nitrate Produced
File: A F F F Inhib (17-11-97).xls Sheet: N 0 3 - & N 0 2 Produced
C-19
US00001144
B N R in h ib itio n T e s ts - 6 0 p p m A F F F P re tre a te d w ith F e n to n 's R e a g e n t (N o v /1 7 /9 7 )
TSS R e a c to r A1 A2 A3 B1 B2 B3
Initial w t 1.114 1.1126 1.1199 1.1154 1.1113 1.0983
Final w t 1.1559 1.156 1.162 1.1586 1.1527 1.1394
Volum e 15 15 15 15 15 15
MLSS 2793 2893 2807 2880 2760 2740
WT @ 550 C 1.1187 1.1191 1.1255 1.1154 1.1172 1.1025
MLVSS 2480 2460 2433 2880 2367 2460
TDS R e a c to r A1 A2 A3 B1 B2 B3
Initial wt 1.0134 1.0147 1.0153 1.0436 1.0465 1.0307
Final w t 1.0261 1.0293 1.0303 1.0945 1.0952 1.0745
Volum e 15 15 15 15 15 15
TDS 847 973 1000 3393 3247 2920
TS R e a c to r A1 A2 A3 B1 B2 B3
Initial wt 1.0312 1.0166 1.0122 1.0083 1.0141 1.0109
Final w t 1.13
1.0746 1.0704 1.1003 1.1064 1.1044
Volum e 25 15 15 15 15 15
TS 3952 3867 3880 6133 6153 6233
X TSS,TD S 3640 3867 3807 6273 6007 5660
File: A F F F Soilds Data
C-20
i
US00001145
BINR In h ib itio n B atch A ssay P r e tr e a te d w ith F e n to n 's R e a g e n t (AFFF ]20 ppm)
C-21
US00001146
R eactor
AFFF (ppm )
H 2O 2
m g/l
A1 0 0
A2 0
0
A3 0
0
B1
120
3000
B2
120
3000
B3
120
3000
Fe** m g/l
0
0 0
300
300 300
S ta g e F e e d s to c k R R Decant E n d of F e e d in g E n d of A n aero b ic E n d o f A e ro b ic E x ten d ed A e ra tio n E n d o f S e ttlin g E n d o f F e e d in g E n d of A n aero b ic E n d of A e ro b ic E n d o f S e ttlin g E n d of F e e d in g E n d o f A n aerobic E n d of A e ro b ic E n d o f S e ttlin g E n d of F e e d in g E n d of A n aerobic E n d of A e ro b ic E x te n d e d A eration E n d of S e ttling E n d of F e e d in g E n d of A n aerobic E n d of A e ro b ic E n d of S e ttling E n d of F e e d in g E n d of A n aerobic E n d of A e ro b ic E n d of S e ttling
Tim e
N H 3-N (m g /L)
2 hr
3 4.6 0.1
2 5.2
4 hr 6 hr (4 h r)
2 2 .1 0 .9 0.1
8 hr 2 hr 4 hr
0.1 26 .3 2 1 .3
6 hr 8 hr 2 hr
1 .0 0 .5 27 .3
4 hr 6 hr
1 8 .9 1 .0
8 hr
0 .5
2 hr 4 hr 6 hr
2 7 .3 1 6 .2
2 .4
<4hr) 8 hr 2 hr
0.1 0.1 2 4 .3
4 hr 6 hr 8 hr
1 4 .4 3 .0
1.6
2 hr 4 hr 6 hr 8 hr
2 6 .3 1 4 .4
2-9 1 .6
TKN (m g/L)
140 .7 1 .8
16 .2 1 6.5
2 .7 2 .6 2 .0 22 .8 23 .3 2 .9 2 .8 38 .0
2 7 .6 2 .9 5 .6
50 .6 29 .2 1 3.4
5 .9 8 .7 4 2 .6 3 4 .6 1 3 .4 1 2.3 56 .6 4 1 .1 12 .0 9 .8
A lkalinity 4 3 0 .0 3 1 9 .0 5 0 1 .0 4 7 3 .0 2 8 7 .0 2 9 7 .0 2 7 1 .0 5 0 6 .0 4 8 4 .0 2 7 3 .0 2 9 1 .0 4 7 3 .0 4 8 7 .0 2 9 4 .0 3 0 0 .0 5 9 2 .0 5 5 4 .0 4 7 5 .0 4 5 7 .0 4 3 3 .0 5 8 4 .0 5 6 0 .0 4 8 7 .0 5 0 3 .0 5 7 8 .0 5 6 4 .0 4 8 5 .0 4 7 7 .0
J
File: A F F F Inhib (1-12-97).xls Sheet: Standards & Data
C-22
US00001147
A FFF H;0 , R e a c t o r (p p m ) mg/1
Fe* mg/1
Stage
F eedstock
R R Decant A 1 0 0 0 E n d of Feeding
En d of A naerobic E n d of A erob ic Extended A eration
E n d of Settling A 2 0 0 0 End of Feeding
E n d of A naerobic
E n d of A erob ic E n d of Settling A 3 0 0 0 E n d of Feeding
E n d of A naerobic E n d of A erobic E n d of Settling
B1
120 3000
300 En d of Feeding
En d of A naerobic E n d of A erobic
Extended Aeration
E nd of Settling
B2
120 3000
300 En d of Feeding
End of Anaerobic
E n d of A erobic
E n d of Settling
B3
120 3000
300 End of Feeding
E n d of A naerobic
E n d of A erobic
E n d of Settling
stand 2 Stand 3
stan d 4 Standards used
STD 1
____!___ 1
STD 2 STD 3 STD 4
Tim e
C lA rea
(m g/L)
no2 N
A rea
(m g/L)
N CV-N A rea
P 0 4 -P (m g /L ) A b s o rb . mg/1
2 hr
6415840997 5165171073 5636490017
2 4 9 .5 2 0 1 .1 2 1 9 .3
0 15252495
0
0 .0 0 .4 0 .0
2999602 980449999
8285053
0 .9 17 .4 1 .0
0 .3 5 9 0 .1 1 0 .5 5 6
1137 3 .9 4 1 7 .2 4
4 hr 5565778827 216.6 2 2 014912
0 .5
8248297
1 .0
1 .5 2 7
4 6 .2 0
6 hr 5 5 8 7 7 67 1 2 4 217.5 237912104 5 .6 13090 05 0 3 0 2 2 .9 0 .1 9 6
6 .5 1
(4 h r) 63608 77 5 4 8 247.3 28977091
0.7 1665166248 2 8 .9 0.165
5 .5 8
8 hr 2 hr 4 hr
5742700331 5453642899 5549230457
2 2 3 .4 2 1 2 .3 2 1 6 .0
14439043 0
22476337
0 .3 1756232544 3 0 .4
0 .0 5348368
1 .0
0 .5 1 2 7 1 9 68 9 1.1
0 .1 0 7 0 .40 9 1.131
3 .8 5 1 2 .8 6 3 4 .3 9
6 hr 5 5 4 5 3 13 2 8 7 215.8 2 4 3723873 5 .7 1218372767 2 1 .4 0 .22 4
7 .3 4
8 hr 5 6 6 3 4 77 2 3 8 220.4 2 5 3297426
5 .9
1078852244
1 9 .0
0 .1 1 2
4 .0 0
2 hr 5 4 9 6 0 24 6 2 0 213.9
0
0 .0
102182
0 .9
1 .0 3
3 1 .3 8
4 hr 6 hr 8 hr
5576772514 5556863735 5951069962
2 1 7 .0 2 1 6 .3 2 3 1 .5
21352132 251352393 324595775
0 .5 59 7 .6
9222295 1228966969 892540865
1 .0 2 1 .6 1 5 .9
1 .1 9 4 0 .0 9 9 0 .2 6
36 27 351 8 .42
2 hr 4 hr 6 hr (4 h r) 8 hr ? hr
457079520 436465012 424172102 462727593 448160769 400203689
2 2 8 .9 2 1 9 .4 2 1 3 .7 2 3 1 .5 2 2 4 .8 2 0 2 .5
0 0 25833324 6404243 3939501 0
0 .2 0 .2 7 .3 1 .8 1.1 0 .2
0 2413048 56300488 36280666 101580036 3064266
0 .9 09 21 9 17 8 31 0 0 .9
1 .4 1 7 1 .5? 1 .1 7 2 0 94 1 064 1 .1 6 6
4292 45 99 3561 2869 3239 3543
4 hr 6 hr 8 hr
395127891 408789981 459889036
2 0 0 .2 206 5 230 2
0 28250426 5272664
02 7 .9 1 .5
3821042 46467575 89906486
0 .9 19 9 28 6
1 669 110? 1 .0 7 4
50 43 3352 32 .6 9
2 hr 4 hr 6 hr 8 hr
412801655 400870390 434292751 424292751
208 4 2 0 2 .8 2 1 8 .3 2 1 3 .7
0 1322995 24585375 11525301
02 0 .2 6 .9 32
5131448 2745177 51478437 88687303
1 .0 0 .9 20 9 28 .4
1 .1 0 5 1 .3 8 5 1 .1 8 3 1 .2 1 3
3361 41 96 3 5 .9 4 3 6 .8 3
2501404503 5127125765
98.12 4 3 4 6 51899 1996 883978295
1 0 .1 8 2 0 .7
7 8 8 6 5 7 0 0 1 7 306.3 1 700383445 3 9 .8
506201128 1094957038 2399022283
9 .3 9 193 4 1 .2
I1
1726964 2497916940 5128148989 7744736801
00 100 430009432 200 883500936 300 1691875383
0 2116836 10 505155875 20 1090488678 40 2354417370
0 0.158 10 0.636 20 1.14 40 1.666
5 20 35 50
File: A F F F Inhib (1-12-97).xls Sheet: Standards & Data
C-23
IC Colum n C I'S tan d a rd C urve
4. O O E-08 1 .4 0 E -K X )
IC C o lu m n N 0 2'- N S ta n d a rd C u rv e
2. O O E-08 -2.3 3E -0 1
1C C o lu m n N O ,'- N S ta n d a rd C u rv e
2.00E-08 8 74E-01
Phosphorus Standard Curve
File: AFFF Inhib (1-12-97).xls Sheet: Standards & Data
C-24
US00001149
SUMM ARY OUTPUT
CL
R eg ression Statistics
R Square
0 .9 9 9 8 5 9 0 1 6
O b servatio ns
4
In te rc e p t
Coefficients S tandard E rror 1 .4 0 3 6 9 9 4 1 7 1 .56137 508
X V ariable 1
3 .8 6 6 5 4 E -0 8 3 .2 4 6 6 E -1 0
SUMM ARY OUTPUT N 0 2 -N
R egression Statistics
R Square
0 .9 9 9 3 8 7 6 6 9
O bservatio n s
4
In te rc e p t X V ariable 1
Coefficients Standard Error
0 0 .40411 631
2 .3 4 1 8 7 E -0 8
4 .1 3 1 E -1 0
SUMMARY OUTPUT N 0 3 -N
R eg ression Statistics
R Square
0 .9 9 7 6 4 1 6 8 8
O bservatio ns
4
In te rc e p t X V ariable 1
Coefficients S tandard Error 0 .8 7 3 4 5 6 7 2 4 0 .7 6 4 6 4 3 1 8 1 .6 8 2 7 7 E -0 8 -5 .7 8 5 3 E -1 0
SUMMARY OUTPUT P 0 4 -P
R eg ression Statistics
R Square O bservatio ns
0 .9 9 9 5 4 3 8 9 3 4
In te rc e p t X V ariable 1
C o e ffic ie n ts :S ta n d a rd E rro r 0 .6 6 2 6 0 4 3 0 .4 7 7 9 8 4 8 2
2 9 .8 1 9 3 2 8 5 6 0 .4 5 0 4 1 7 4 6
File: A F F F Inhib (1-12-97).x!s Sheet: Standards & Data
C-25
US00001150
R eactor
AFFF (ppm )
H 202 m g/1
A1 0
0
A2 0
0
A3 0
0
B1 120 3 0 0 0
B2 120 3000 B3 120 3000
Fe3+ m g/l
0
0 0 300
300 300
S ta g e F e e d s to c k R R Decant E n d of F ee d in g -- E n d of A n a e ro b ic '" E n d o f A ero bic E x ten d ed A eration E n d of S ettling E n d of F e e d in g " E n d of A naerobic ** E n d of A e ro b ic E n d o f S ettling E n d of F e e d in g " End of A n a e ro b ic " E n d of A e ro b ic E n d o f S ettling E n d of F e e d in g " E n d of A n aerobic** E n d of A ero bic E xten ded A eration E n d erf S e ttlin g E n d of F e e d in g -* E nd of A n aero b ic-* E n d of A ero bic E n d of S ettling E n d of F e e d in g -- E n d of A n aerobic E n d of A ero bic End of S ettling
STD 1 STD 2 STD 3 STD 4 STD 5
Tim e
2 hr 4 hr 6 hr (4 h r) 8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr (4 h r) 8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr 8 hr
COD
ABS
0 .0 8 < 0 .0 0
1 0 8 2 .'\ 54.1
0 002
27.1
0 .0 0 1
1 3 .:
0 .0 0 5
67.7
0 .0 0 2
2 7 .1
0 .0 0 1
1 3 .5 1
0 .0 0 2
2 7 .1
0 .0 0 1 0.0 0 1
1 3 .5 1 3 .5
0 .0 0 1
1 3 .5
0 .0 0 1
1 3 .5
0 001
1 3.5
0 .0 0 1
13 5
0 .0 0 1
1 3.5
0 029
3 9 2 .4
0 .0 2 9 0 027
3 9 2 .4 3 6 5 .3
0 .024
3 2 4 .7
0 .0 2 5
3 3 8 .3
0 028 0 .0 4 0 0 024 0 .0 2 4
3 7 8 .8 5 4 1 .2 3 2 4 .7 3 2 4 .7
0026 0 023 0 021
3 5 1 .8 3 1 1 .2 2 8 4 .1
0 021
2 84 .1
0 000 0 077
0 1000
0 ,1 4 7 2 0 0 0
0 225 3000
0330 4500
.
932 9 6 .6 8 3 .0 93 .2 QRK 93 .2 966 9 6 .6 96 S 9 6 .6 966 9 6 .6 96 6 69 .3 69 .3 7 1 .4 74 5 735 7 0 .3 5 7 .6 74 6 74 6 7 2 .4 756 7 7 .7 77 7
F S (F iltered )** FS A verage"
0 .0 8 0 0 .0 8 0
108 2 .4 1 0 8 2 .4
R R S U (F ilte re d )**
0 .0 0 4
5 4 .1
f
R K S U A verage**
0 .0 0 4
5 4 .1
T he v a lu e s of "C O D % R em o v al" show n in tab le a n d ch art ab o v e are accum ulative figures b a se d on the initial COD co n centration at tim e 0 hr.
File: AFFF Inhib (1-12-97).xls Sheet: COD
C-26
US00001151
S a m p le C o ntrols (A W 5 A 3 )
Initial CO D at T im e 0 hr.
C onstituent R R Decant F e e d s to c k AFFF T o ta l
V d (L ) 4 2 0
C O D m g /L 5 4 .1
1 0 8 2 .4 0
---------- - J
Inhibitio n (B 1 .B 2 & B 3 )
R R Decant F e e d s to c k AFFF
4 54.1 2 1 0 8 2 .4 2 2 6 3 8 .3 5
R a w A F F F C O D R e d u c e d by Fentons R e ag en t T o ta l
2 1 6 .4 8 21648
0 3 9 6 .9
2 1 6 .4 8 2 1 6 4 ,8 5 2 7 6 .7
R e aclo r (AFFF ppm ) % COO Removal
A1 (0) 9 6 .6
A 2 (0) 9 6 .6
A 3 (0) 9 6 .6
B1 (60) 7 3 .5
% COD Removaf
B 2 (60) 7 4 .6
B 3 (60) 7 7 .7 .
o E
oc o
o
o
R e acto r (A F F F ppm )
COD Standard Curve for High Range Vials
File: A F F F Inhib (1-12-97).xls Sheet: C O D
C-27
m - 13530
US00001152
Fentons Pretreatment
F Of A F F F p p m
T a p w ater A ctu al A F F F ppm
H 2 0 2 , 3 0 0 0 *2 .5 m g/l F e3 + 3 0 0 *2 .5
F e S O t (3tm es)
120 R eactor 1
1250 ml 300 10m l
7 5 0 0 m g/l 7 5 0 m g/l 3 7 2 3 .2 8 mg/1
120 R eactor 2
1250 ml 300 10m l
7 5 0 0 m g /l 7 5 0 m g/l 3 7 2 3 .2 8 m g/l
120 R eactor 3
1250 ml 300 10m l
7 5 0 0 m g /l 7 5 0 m g /l 3 7 2 3 .2 8 m g/l
Initial C O D
J2X
AFFF
ppm
A b so rb a n c e 0 .2 5 1
C O D m g /I 3 3 9 6 .0 3
A ctu al C O D 3 3 9 6 .0 3
C O D after 2 4 hr reaction period betw een fentons reag e n t a n d A F F F
% C O D RemcwaJ
A b so rb a n c e 0 .195
C O D m g /l 2 6 3 8 .3 5
A c tu a l C O D 2 6 3 8 .3 5
2 2 .3 1
File: A F F F Inhib (1-12-97).xls Sheet: C O D
C-28
I
US00001153
D issolved O xygen
A 1 EJA2 A 3 DB1 DB2 B3
File: A F F F Inhib (1-12-97).xls Sheet: Oxygen Uptake
C-29
US00001154
S tage F e e d in g A n aerobic A e ro b ic
A1 0 .1 1 0.0 7 5 .58
Dissolved Oxygen (m g/L) at various stages
A 2 A 3 B1 B2
0 .0 9
0 .0 9
0.0 9
0 .0 8
0 .0 8
0 ,0 9
0.0 9
0 .1 0
5 .8 4
6.1 7
6 .1 0
5.5 7
B3 0 .1 0 0 .0 9 5 85
S tage A ero bic
T im e (sec) 0
30 60 90 120 150 180 210 240 270 300 330 360 390 420 450 480 510 540 570 600
A1 8 .02 6.4 9 5.8 6 5.5 0 5 .18 4.9 0 4.6 6 4.4 0 4.1 8 3.9 8 3 .7 9 3 .6 0 343 3 27 3.1 0 Z95 2.8 3 2.6 9 2.5 6 2 .4 4 2 .32
D is s o lv e d O x y g e n in m g /L
A 2 A 3 B1
8 .4 3
7 .2 0
7 .6 1
5 .7 7
5.4 2
6 .2 6
5 .1 2
4 .9 6
5.7 2
4 .6 8
4 .5 4
5 .3 5
4 .3 2
4 .2 5
4 .9 8
4 .0 2
3.9 7
4 .6 7
3 .7 4
373
4 .3 7
3.4 7 3 .2 3
3 .5 1 3.2 7
4 .1 1 3 83
2 .9 9 2 77 2 .5 8 2 38 2 .2 2 205 1 89 1 .75 1 .6 3
3.0 7 2 .8 8 2 71 2 .5 4 2 39 2 24 2 .1 0 1 .9 7 1 .8 6
3 .5 8 3 .34 3 .1 3 2 .9 1 2 72 2 51 2 32 2.1 6 1 .98
1 .4 8 1 .3 7 1 .2 7
1 .7 4 1.6 3 1 .5 4
1.8 5 1 68 1 .55
B2 7 .5 6 6 .3 7 5 .7 7 5 .3 1 4 .9 0 4 .5 2 4 .17 384 3 54 3 .2 5 2.9 8 2 .7 3 2 .4 8 2 .2 6 2 .0 4 1 .8 4 1 .6 6 1 .4 8 1 .2 9 1 .1 5 1.01
B3 8 .2 5 6 .3 8 5 .6 6 5 .1 6 4 .7 6 4 .3 5 4 .0 6 3 .7 1 3 .3 9 3 .11 2 .8 3 2 59 2 .3 6 2 13 1 .9 2 1 .7 2 1.5 4 1 .3 8 1 .2 1 1 .0 6 0 .9 2
File: A F F F lnhib-(1-12-97)..xs Sheet: Dissolved Oxygen
C-30
l
US00001155
R eactor
AFFF (ppm )
H 202 m g/t
A1 0
0
A2 0
0
A3 0
0
B1
120
3000
B2
120
3000
B3
120
3000
Fe3+
m g/l
S ta g e F e e d s to c k
R R Decant 0 E n d o f Fee d in g
E n d of A n aerobic
E n d o f A e ro b ic
E x te n d e d A eration E n d c f S ettling 0 E n d o f Fee d in g
E n d erf A n a e r o b ic
Tin TKN
140/
2 hr 4 hr 6 hr (4 b r) 8 hr 2 hr 4 hr
1.6 16/ 15.5
2 .7 2 .6 2.C 2 2 .8 2 3.3
0 300
E n d o f A erobic E n d o f S ettling E n d erf F e e d in g E n d of A n aerobic E n d erf A e r o b ic E n d o f S ettling E n d o f F ee d in g E n d erf A n a e r o b ic E n d o f A ero bic
6 hr 8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr
2 .9 2 .8 3 8 .0 27 .6 2 .9 5 .6 50 .6 2 9 .2 13.4
300 300
E x te n d e d A eration E n d o f Settling E n d of F eeding End of A n aerobic End of A erobic End o f S ettling E n d of Feeding End of Anaerobic
(4 h r) 8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr
5 .9 8 .7 4 2 .6 34 6 1 3 .4 1 2 .3 5 6 .6 4 1 .1
End of A ero bic End of S ettling
6 hr 3 hr
120 9 .8
N H 3 -N 34. 0.1 25/ 2 2 .1 OS 0 .1 0 .1 26 3 2 1.3 1 .0 0 .5
2 7 .3 18 .9
1 .0 0 .5 27 .3 1 6 .2 2 .4 0.1 0 .1 24 .3 1 4 .4 30 1 .6 26 3 14.4 2 .9 1 .6
O rg. N 106.C 1.7 -9.C -5 .6 1.5 2 .5 1.S -3 .5 2 .0 1 .9 2 .3 1 0 .7 8 .7 1 .9 5 .1 2 3 .3 1 3.0 1 1.0 5 .8 8 .6 1 8 .4 2 0 .2 1 0 .4 10 .6
30 4 26 .7
9.1 8 .2
C o n e ntration, m g /L
N 0 2 -N
N 0 3 -N
o.c o.s
0 / 17.4
o.c 1.C
Total N 1 4 1 .6 19 ) 17/
2 4 9 /> 201 ' 219/
1 1 .4 39 1 7 .2
0 .5 1.C 18.C 2 1 6 .6 4 6 . 2
5.6
22.S
31.2 2 17 .5
6 .5
0 .7
2 8 .9
32.2 2 4 7 /
5 .6
0.3 3 0 .4 32.8 7 2 3 4 3 9
0.0 1.0 23.8 212 .3 12.9 0 .5 1.1 2 4 .9 2 1 6 .0 3 4 .4 5.7 2 1 .4 3 0 .0 2 1 5 .8 7 .3 5 .9 1 9.0 2 7 8 7 2 0 4 4 j)
0 .0 0 .9 3 8.9 2 1 3 .9 31.4 0 5 1.0 29.1 2 1 7 .0 3 6 3 5 9 2 1 .6 3 0 ,3 2 1 6 .3 3 .6 7 .6 1 5 .9 2 9 1 731 5 A 4
0 .2 0 .9 5 1 .6 2 2 8 .9 4 2.9 0 .2 0 .9 30 .3 2 1 9 4 46 .0 7 .3 2 1 .9 4 2 .5 2 1 3 .7 35.6 1.8 17.8 2 5 .5 2 3 1 .5 28.7 1.1 3 1 .0 4 0 8 2 2 4
0 .2
0 .9
4 3 .8 2 0 2 5 35.4
0 2 0 .9 3 5 .6 2 0 0 2 50.4
7 .9
1 9 .9
4 1 .2 2 0 6 .5 3 3 .5
1.5 2 8 .6 4 2 4 7 3 0 7
02 0 .2 6 .9 3 .2
1 .0 0 .9 20 9 2 8.4
57 .8 42 .2 39 .8 4 1 .4
208 .4 3 3 6 2 0 2 8 4 2 .0 218 3 35 939 2 1 3 .7 3 6 .8 3 3
File: A F F F Inhib (1-1 2-9 7).x!s Sheet: Concentrations T able
C-31
US00001156
R eactor A1
pa
A3 B1
B2 B3
AFFF
(ppm )
S ta g e
F e e d s to c k
Tim e
R R Decant 0 E n d of F e e d in g
2 hr
E nd of A n aerobic E n d of A e ro b ic E xten ded A eration
4 hr 6 hr (4 h r)
E n d of S e ttling 0 E n d of F e e d in g
E n d of A n aerobic E n d of A e ro b ic
8 hr 2 hr 4 hr 6 hr
E n d o f S ettling 0 E n d of F e e d in g
8 hr 2 hr
E n d of A n aero b ic
4 hr
E n d of A e ro b ic
6 hr
120
E n d erf S e ttlin g E n d erf F e e d in g
8 hr 2 hr
E n d of A n aero b ic
4 hr
E n d of A e ro b ic
6 hr
120 120
E xten ded A eration E n d of S ettling E n d of F e e d in g E n d of A n aerobic E n d of A e ro b ic E n d of S ettling E n d of Fee d in g E n d of A n aerobic E n d of A e ro b ic E n d of S ettling FS1
<4hr) 8 hr 2 hr 4 hr 6 hr 8 hr ? hr A hr 6 hr 8 hr
FS2
FS3
FS Avarage
RRSU1
RRSU2
RRSU3
R R S U Avarage
C o n c e n tra tio n , m g /L
BOD
COD
TOC
108 2 .4 5 4 .1
2 8 7 .7 1 1.5
2 7 .1 13 .5
1 2.5 1 3 .2 9
67 .7 2 7 .1 13 .5 2 7 .1
11.4 8 1 1 .34
11 .5 1 2.8
13 .5 .1 3 .5
1326 1 1.6
1 3.5 13 .5
11 5 10 .6
13 .5
1 1.7
13 .5
1 1 .1
1 3 .5 3324
1 1 .4 1 4 0 .5
3 9 2 .4 1 2 5 .8
3 6 5 .3 1 3 5 .7
3 2 4 .7 1 3 1 .9
338 3 3 7 8 .8
1 3 3 .2 1 5 5 .9
5 4 1 .2 324 .7 3 2 4 .7
,1 3 1 .5 1 2 2 .5 122 .4
351 8 31T 2
1 6 1 .2 1 3 8 .5
2 6 3 .3 263 3
139 2 1 3 2 .0
287 .1
2 8 8 .9
287 1
2 8 7 .7
12 .0
1 1 .2
1 1.3 1 1 .4 8
| A lkalin ity
4 3 0 .0 3 1 9 .0 5 0 1 .0 4 7 3 .0 2 8 7 .0 2 9 7 .0 271 0 5 0 6 .0 4 8 4 .0 2 7 3 .0 2 9 1 .0 4 7 3 .0 4 8 7 .0 2 9 4 .0 3000 5 9 2 .0 5 5 4 .0 475 0 4 5 7 .0 433 0 5 8 4 .0 560 0 4 8 7 .0 503 0 5 7 8 .0 564 0 485 477
.
File: AFFF Inhib (1-12-97).x!s Sheet: Concentrations Table
C-32
.* U S 0 0 0 0 1 157
inhibition Effect of AFFF on Nitrification in terms of NH3-N
-- -- At -- 3B-- A 2 --A-- A3 -- B1
B2 -- B3
Inhibition effect of AFFF on Nitrification in terms of N03-N
-A 1 -A 2 -A 3 - B1 -B 2 -B 3
Inhibition effect of AFFF on Nitrification as of TKN
A1
A2 -A-- A3
B1 B2 | -- >-- B 3
File: AFFF Inhib (1-12-97).xls Sheet: NH3-N & TKN Charts
C-33
U S 0 0 0 0 1 158
File: AFFF Inhib (1-12-97).xls Sheet: NH3-N & TKN Charts
C-34
U S 0 0 0 0 1 159
R eactor A1 (No AFFF present)
S tage
Reactor A2 (No AFFF present)
S tage
File: A F F F Inhib (1-12-97).xls Sheet: Nitrogen Mass Balance
C-35
US00001160
60.0
_50.0
o>
40.0
5 30.0
o>
J 20.0
2 10.0
0 .0 -
R eacto r B1 (120p pm A F F F-F en ton treated)
H
E n d of F ee d in g
End of A n aero b ic
End of A erobic
S tag e
E nd of S ettling
N 0 3 -N N 0 2 -N B O rg. N N H 3-N
4 5 .0 4 0 .0
S 3 5 -0 3 0 .0 = 2 5 .0
o>20.0
2 15 .0
z 10.0
5 .0 0 .0
R eactor B2 (120ppm AFFF - Fenton treated)
N 0 3 -N
I D N 0 2 -N
lO rg. N
O N H 3 -N
End of F ee d in g
End of
End of
A n aerobic
A erobic
S tag e
End of S ettling
Reactor B3 (120ppm A FFF- Fenton treated)
E n d of F e e d in g
End of A n aero b ic
S tag e
0 N 0 3 -N N 0 2 -N B O rg. N N H 3-N
E n d of S ettling
File: A F F F Inhib (1-1 2-9 7).xls Sheet: Nitrogen M ass Balance
C-36
US00001161
R eacto r A1 (C o n tro l)
AFFF ppm 0
H202
m g /l 0
m g /l 0
A2 (C ontrol)
0
A3 (C ontrol)
0
B1 1 20
0 0 3000
0 0 300
B2 120
3000
300
B3 120
3000
300
Fe3+
S tag e E n d of Fee d in g E n d of A n aero b ic E n d of A e ro b ic E x ten d ed A eration E n d of S ettling E n d of F e e d in g E n d of A n aero b ic E n d of A e ro b ic E n d of S e ttlin g E n d of Fee d in g E n d of A n aero b ic E n d of A e ro b ic E n d of S e ttlin g E n d of F e e d in g E n d erf A n a e r o b i c E n d of A e ro b ic E x ten d ed A eration E n d of S ettling E n d erf F e e d in g E n d erf A n a e r o b ic E n d of A e ro b ic E n d of S e ttling E n d of Fee d in g E n d of A n aerobic E n d erf A e r o b ic E n d erf S e ttlin g
Tim e 2 hr 4 hr 6 hr (4 h r) 8 hi 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr (4 h r) 8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr 8 hr
Nitrogen Concentration, mg/L
N H 3-N N 0 3 --N
N 0 2 -N A (N O j-N )
25 23
10
00
2 2 .1 0
10
0 .5
0.8 5
229
5 .6
0 .1 2
28 .9
0 .7
007
3 0 .4
0 3 29.41
2625
10
0 .0
2 1 .2 6
11
05
1 .0 3
2 1 .4
5 .7
0 51
190
5 .9 18.0 6
2 7 .3 1
09
0 .0
18 93
. io
0 .5
0 99
21 .6
5 .9
0.4 7
1 5 .9
7 .6 15.0 2
27 30
09
02
1 6 .22
09
02
2 .4 1
21 9
73
0.1 0
178
1 .8
009
31 0
1 1 3 0 .1 2
2425
09
02
1 4 .4 4
09
0 .2
3 .0 4
199
79
1 .6 2
28 6
1 .5 18.94
26 25
10
02
1 4 .4 4 2 92
09 1 2 .5 0
02 4.1 7
1.6 2
1 4 .0 1
0 47
1 1 .5 4
A m m onia R em aining (Supem atent)
Nitrate (Supernatant)
File: A F F F Inhib (1-12-97).xls Sheet: N O S- & N 0 2 Produced
C-37
US00001162
B N R In h ib itio n T e s ts - 120 p p m A F F F w ith F e n to n 's T re a tm e n t (D e c /1 /9 7 )
TSS R e a c to r A1 A2 A3 B1 B2 B3
Initial wt 1.0941 1.0988 1.0985 1.0895 1.0905 1.0951
Final wt 1.1387 1.1444 1.1424 1.133 1.1346 1.1371
Volum e 15 15 15 15 15 15
MLSS 2973 3040 2927 2900 2940 2800
W T @ 550 C 1.0993 1.1035 1.1028 1.0933 1.0948 1.1
MLVSS 2627 2727 2640 2647 2653 2473
TDS R e a c to r A1 A2 A3 B1 B2 B3
Initial wt 1.0026 1.0032 0.9989
. 1.0095 1.0013 0.9993
Final w t 1.0181 1.0186 1.0134 1.073 1.0626
1.05
Volum e 15 15 15 15 15 15
TDS 1033 1027 967 4233 4087 3380
TS
R e a c to r A1 A2 A3 B1 B2 B3
Initial wt 1.0128 1.0051 0.9958 1.0042 0.9972 0.9995
Final wt 1.0728 1.0646 1.0556 1.1127 1 1077 1.1048
Volum e 15 15 15 15 15 15
TS 4000 3967 3987 7233 7367 7020
ETSSjTD S 4007 4067 3893 7133 7027 6180
File: AFFF Soilds Data
C-38
US00001163
B N R Inhibition B atch A ssay P re tre a te d w ith F e n to n 's R eag en t (A FFF 480 ppm)
C-39
US00001164
A FFF H ,0 ?
R eacto r \ (p p m ) | m g/l --------------- :------------
-i ,I
A1 * 0
0
! \
A2 ! 0 ! ' :
A3 : 0
0 0
i
i j
B1 i 480
3000
82 ' 480 !
1 1 i 83 j 480
I
3000 3000
F e 3' m g/l
Stag e
Tim e
C lA rea
(m g/L)
n o 7*-N
A rea
(m g /L)
n o 3 -n A rea
P04-P (m g /l.) A b so rb .
^ Fe ed sto ck R R Decant 0 En d o l Feed ing
2 hr
6994915305 6143498102 6342217404
2 6 7 .2 2 3 4 .7 24 2 .3
0 0 0
0 .0
6898590
0 .9
0 .0
152448200
3 .4
0 .0 1 6 1 3 5 8 5 3 1.1
0 .3 6 6 0 .0 5 7 0 .8 8 9
E n d ol A naero bic lE n d o l A ero b ic Exten d ed A eration
4 hr 6 hr (4 h r)
6314295331 6564722520 6360877548
24 1 .3 25 0 .8 2 4 3 .0
16684479 207690276 28977091
0 .4 4 .8 0 .7
340286209 1029993807 1665166248
65 1 8 .1 2 8 .8
1 .0 6 7 0 .1 9 5 0 .1 0 7
E n d o l Settling 0 End of Feed ing
E n d of A naero bic
8 hr 2 hr 4 hr
6 4 4 2 9 9 5 9 2 0 2 4 6 .2 6 2 9 5 3 8 8 3 6 2 j 2 4 0 .5 62812 76 8 5 1 2 4 0 .0
0 0 0
0 .0 1 7 7 4 3 55 8 9 3 30 .7
0 .0
7701686
0 .9
0 .0 5558668 0.9
0 .0 2 6 1 .1 2 9 1 .2 6 5
E n d of A ero b ic E n d o l Settling 0 En d of Feed ing
6 hr 8 hr 2 hr
6463991289 6324538637 6404002120
2 4 7 .0 2 4 1 .6 2 4 4 .7
224767195 200313133
0
5 .2 4 .6 0 .0
756122193 776512216
4154920
135 1 3 .9 0 .9
0 .3 2 4 0 .1 4 5 1.041
|E n d ol A naero bic E n d o l A ero b ic E n d of Settling 300 En d of Feed ing En d ol A naero bic E n d o l A ero b ic
4 hr 6 hr 8 hr 2 hr 4 hr 6 hr
6377294440 6441725036 6553391763 463427330 438079340 513179469
2 4 3 .7
0
2 4 6 .1 2 5 0 .4
188674712 185443858
215 8
0
204 2 2 3 8 .6
0 6113100
0 .0 4 .3 4 .3 0 .0 00 0.1
17260157 725441683 844270868
3630164 2413048 17456452
1.1 13 0 15 .0 0 .8 0 .8 13 .0
1 .1 6 4 0 .0 7 1 0 .0 9 5 1 .5 3 3 1 .7 2 6 1 .0 9 8
E xtend ed A eration E n d of Settling 300 En d ol Feed ing En d ol A naero bic E n d o l A etob ic E n d ol Settling 300 E n d ol F eeding . E n d ol A naero bic E n d ol A ero b ic E n d o l Settling
(4 h r) 8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr6 hr 8 hr
462727593 450324509 452867340 449852557 459782056 459889036 456854052 43S048021 453387705 475054749
2155 2 0 9 .8 2 1 1 .0 2 0 9 .6 2 1 3 .9 214 2 212 8 2 0 4 .2 211 2 2 2 1 .1
6404243 5303037
0 0 9635275 5272664 0 1322995 6628273 11525301
0.1 0.1 0 .0 0 .0 0 .2 0.1 0 .0 00 0 .2 0 .3
36280666 37543352 3064266 3821042 10084217 8990648 5131448 2745177 152314B8 22687303
168 17 .0 0 .8 08 1 1 .5 11.2 0 .9 0 .8 12 5 14 .0
0 .9 1 4 0.951 1.121 0.781 0 .7 5 4 0 .7 9 7 1.247 1.195 0 .6 3 5 1 317
stand 2 Stand 3
j
2 5 0 9 6 98 1 7 1 9 6 .0 7 434368072 9 .9 6
503394837
9 .2 6
j 5 2 6 2 3 0 3 2 2 6 | 201.1 1 9 0 8 908419 j 2 0 .8 [ 1 1 0 9 9 1 3 7 1 8 j 1 9 .5
stan d 4 Stand ard s u sed ! STD 1 ! STD 2 I STD 3 ! STD 4
78397 59 6 4 1 \ 2 9 9 .5 1.693E+ 09 ! 3 8 .8 2 3 5 8 731472 ! 4 0 .5
24841841 2S78366836j 5272237045j 7838874180J
00 100 442549719 200 913151766 300 1 .7 2 2 E *0 9
0 71633 10 510852474 20 1106408486 40 2352579746
0 0.163 10 0.628 20 1.149 40 1.631
m g/l 1 1 .4 6 2 .06 2 7 .3 9 3 2 .8 0 6 .2 6 3 .5 8 1 .1 1 3 4 .6 9 3 8 .8 3 1 0 .1 9 4 .7 4 3 2 .0 1 3 5 .7 5 2 .4 8 3 .2 1 4 6 .9 9 5 2 .8 7 3 3 .7 5 2 8 .1 5 2 9 .2 7 3 4 .4 5 2 4 .1 0 2328 2 4 .5 9 38 28 3 6 .7 0 1 9 .6 5 4 0 .4 2
5 20 35 50
File: A F F F Inhib (24-11-97).xls Sheet: Standards & Data
C-40
..
J
:
US00001165
A FF F HjOj
R eselo ? (p p m ) --------------- 1------------
,
m g/l
A1 : 0
0
I I
A? \ 0
0
A3 ; 0
0
' 1I B1 t 480
3000
.!
B? ; 480
I 1 ) I 1 B3 1 480
j i
3000 3000
: \ i !
F e 3* m gti
Stag e
F e e d sto ck R R Decant 0 E n d of F e e d in g
E n d of A naero bic E n d of A e ro b ic [Extended A eration
E n d of Settling 0 E n d of Feed ing
[E n d of A naero bic
E n d of A erob ic
E n d of Settling 0 E n d of Feed ing
E n d of A naero bic E n d of A ero b ic E n d of Settling
3 0 0 E n d of F e e d in g
E n d of A naero bic E n d of A ero b ic
300
Extend ed A eration E n d of Settling E n d o! Feed ing
E n d of A n a e ro b ic E n d of A erob ic 1E n d of S e ttlin g 300 En d of Feed ing En d of A naero bic En d of A erob ic
En d of Settling
stand 2 Stand 3
stan d 4 Stand ard s u sed
STD 1
STD 2 1; S T D 3
STD 4
Tim e
2 hr 4 hr 6 hr (4 h .) 6 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr (4 h i) 8 hr ? hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr 8 hr
C l-
A rea
(m g/L)
N O ,' N
A rea
(m g/L)
N O j'-N A rea
P 0 4 -P (m g/L) A bsorb .
mg/T
6994915305 614349810? 6342217404
2 6 7 .2 23 4 .7 24 2 .3
0 0 0
0 .0 6898590 0 9
0 .0
152448200
3 .4
00
16135853
1.1
0 .3 6 6 0 .0 5 7 0 .8 8 9
1 1 .4 6 2 .0 6 2 7 .3 9
6314295331 6564722520 6360877548
241 3 25 0 .8 2 4 3 .0
16684479 207690276 28977091
0 .4 4 .8 0 .7
340286209 1029993807 1665166248
65 1 8 .1 2 8 .8
1 .0 6 7 0.195. 0 .1 0 7
3 2 .8 0 6 .2 6 3 .5 8
6442995920 2 4 6 .2 '
0
0 0 1774355893 3 0 .7
0 .0 2 6
1.11
6295388362 6281276851
2 4 0 .5 2 4 0 .0
0 0
0 .0
7701686
0 .9
1 .1 2 9
3 4 .6 9
0 .0
5558668
09
1 .2 6 5
38 83
64 6 3 9 91 2 8 9 2 4 7 .0 224767195
5 .2
756122193
1 3 .5
0 .3 2 4
1 0 .1 9
6324538637 6404002120
2 4 1 .6 24 4 .7
200313133 0
4 .6 0 .0
776512216 4154920'
1 3 .9 0 .9
0 .1 4 5 1.041
4 .7 4 3 2 .0 1
6377294440 6441725036 6553391763
24 3 .7 2 4 6 .1 2 5 0 .4
0 188674712 185443858
00 43 4 .3
17260157 725441683 844270868
1.1 1 3 .0 1 5 .0
1 .1 6 4 0 .0 7 1 0 .0 9 5
35 .7 6 2 .4 8 3.21
4 6 3 4 2 73 3 0 21 5 .8
0
00
3630164
0 .8
1 .5 3 3
4 6 .9 9
43 8 0 7 93 4 0 20 4 .2
0
00
2413048
0 .8
1 .7 2 6
5 2 .8 7
51 3 1 7 94 6 9 2 3 8 .6 6113100
01
17456452
130
1 .0 9 8
3 3 .7 5
4 6 2 7 2 75 9 3 2 1 5 .5 640 4 2 43
01
36280666
16 8
0 914
2 8 .1 5
45 0 3 2 45 0 9 2 0 9 .8 5303037
01
37543352
1 7 .0
0.951
2927
4 5 2 8 67340 21 1 .0
0
00
3064256
08
1.171
3 4 .4 5
449852557
2 0 9 .6
0
0 .0 3821042 0 8 0 7 8 1 2 4 .1 0
459282056 459889036
2 1 3 .9 2 1 4 .2
9635275 5272664
02 0.1
1008^217 8990648
1 1 .5 1T2
0 .7 5 4 0 797
23 28 2 4 .5 9
456854052 438048021 453387705
212 8 20 4 .2 2 1 1 .2
0 1322995 6628273
00 0 ,0 02
5131448 2745177 15231488
0 .9 0 .8 1 2 .5
1.247 1.195 0 .6 3 5
3 8 .2 8 3 6 .7 0 1 9 .6 5
4 7 5 0 5 4 2 4 9 221.1 11525301
03
22687303
1 4 .0
1 317
4 0 .4 2
2509698171 5262303226
96 .0 7 2 0 1 .1
7839759641 ? 2 9 9 .5
434368072 908908419 1 .69 3 E *09
9 .9 6 2 0 .8 3 8 .8
503394837 1109913718 2358731472
9 .2 6 1 9 .5 40 .5
11
2484184 2578366836j 5272237045] 7838874180]
0 0. 100 442549719 200 913151766 3 0 0 1.722E+ 09
0 71633 10 510852474 20 1106408486 40 2352579746
0 0.163 5
10 06 2 8
20
2 0 1.149 40 1.631
35 50
File: A F F F Inhib (24-11-97).xls Sheet: Standards & Data
C-41
US00001166
IC Column Cl S ta n d a r d Curve
C f C o n e , m g/1
IC C o lu m n N 0 2'- N S ta n d a rd C u rv e
4.00E -08 2.98E -01
2.00E -08 -3.34E -01
1C C o lu m n N 0 3 - N S ta n d a rd C u rv e
2.00E -08 7.85E -07
File: A F F F Inhib (24-11-97).xls Sheet: Standards & Data
j
C-42
US00001167
SUMMARY OUTPUT CL
Regression Statistics
R Square
0 .9 9 9 9 1 1 8 0 4
O bservatio ns
4
In te rc e p t X Variable 1
Coefficients tandard E rro r 0 298 112729 1.24079 601 3.81601 E -08 2 .5 3 4 2 E -1 0
SUMMARY OUTPUT N 0 2 -N
Regression Statistics
R Square
0 .9 9 6 8 7 2 2 0 7
O bservatio ns
4
In te rc e p t X Variable 1
Coefficients tan d ard E rro r 0 055036653
2 .2 9 2 1 5E -08 5 .5 0 7 3 E -1 0
SUMMARY OUTPUT N 0 3 -N
Regression Statistics
R Square
0 .9 9 8 2 9 1 5 5 4
O bservatio ns
4
In te rc e p t X V ariable 1
Coefficients ta n d a rd E rro r 0 785241086 0 .6 5 2 6 2 9 3 9 1 .6 8 4 1 4E -08 4 .9 2 6 5 E -1 0
SUMMARY OUTPUT P 0 4 -P
Regression Statistics
R Square O bservatio ns
0 .9 9 9 5 6 8 5 3 4 4
In te rc e p t X V ariable 1
Coefficients tan d ard E rror 0 .32137 639 0 .4 6 9 1 4 9 1 6
3 0 .4 4 3 7 1 1 6 9 0 .4 4 7 2 4 9 3 4
File: AFFF Inhib (24-11-97).xts Sheet: Standards & Data
C-43
US00001168
Date of Tesl: 3-25-97
COD Concentration and % Removal
AFFF Cone. = 60 ppm
Reactor
AFFF (ppm ) |
H 202 m g/l
. Fe3+ m gll
A1
0|
0
0
\
O O o
A2
i
i .,
;
i j
i !.
A3 o i o ; o
f; i1 i. 1 ; 1i
B1 4 8 0 j 3 0 0 0 ! 3 0 0
J
;;
i; | :
B2 480 i 3000 ! 300 i\ I:
i B3 480 i 3000 ; 300
i:
i
1. ; i: > ;
':
i! J?
i
.' , f >
j | Slage F e e d s to c k R R Decant E nd of F ce d in g E n d of A n aerobic E n d of A erobic E x ten d ed A eration E n d of Settling
E n d of Feeding E n d of A n aerobic E n d of A ero bic E n d of S ettling E n d of Feeding E n d of A n aerobic E n d of A erobic E n d of Settling E n d of Feeding E n d of A n aero b ic E n d ol A ero bic E x ten d ed A eration [E n d of S ettling jE n d of Feeding iE n d of Anaerobic E n d of A erobic E n d of Settling E n d of Feeding E n d of A n aero b ic E n d ol A ero bic E n d ol S ettling
STD 1 STD 2 STD 3 STD 4 STD 5
F S (F iltered )" FS A verage"
R R S U (F ilte re d )" R R S U A verage"
i i Tim e i | . 2 h t ; 4 hr ' 6 hr (4h r)
8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr (4h r) ' 8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr 8 hr
AB5
COD (m g /L ) j V* C O D R e m o v a l
0 .0 7 9 ! 1 0 3 7 .9
0 0 0 1 1 13.1
0 .0 0 6 I 0006j 0 .004 j 0 .0 0 1
7 8 .8 7 8 .8 5 2 .6 1 3 .1
7 7 .2 77 .2 84 .8 96 .2
0 .0 0 2 0 .017 0 .004 0 .004 0 .003
2 6 .3 ' 2 2 3 .3
5 2 .6 5 2 .6 3 9 .4
92 .4 3 5.4 84 .8 848 8 8 .6
0 ,008 0 .004
105 .1 52 .6
69 .6 84 .8
0 .0 0 1 0 .0 0 1
131 13.1
96 .2 9 6.2 .
0 .2 3 2 0217 0202 0195
3 0 4 8 .0 2 8 5 0 .9 26539 2 5 6 1 .9
9.1 15 .0 20 .8 23 .6
0 .1 9 6 0 .2 4 0 0219 0 210 0204 0220 0 .220 . 0.221 0 .2 0 6
2 5 7 5 .0 | 315 3 .1 2 8 7 7 .2 27590 2 6 8 0 .2 2 8 9 0 .4 2 8 9 0 .4 2 9 0 3 .5 2 7 0 6 .4
2 3 .2 59 1 4.2 1 7 .7 2 0 .0 13 8 1 3 .8 1 3 .4 1 9 .3
0 .0 0 0
0
0 .0 8 3 1000
0 .1 6 0 0 .2 3 0
2000 3000
0 .3 3 6 4 5 0 0
0 .0 7 9 0 .079
1 0 3 7 .9 1 0 3 7 .9
0 .0 0 1 0 .0 0 1
13.1 13.1
i 1
* The values of " C O D % R e m o v a l" shown in table and chart above are accum ulative figures based on the initial C O D concentration at tim e 0 hr.
File: AFFF Inhib (24-11-97).xls Sheet: C O D
C-44
US00001169
j a l ' . Test: 3-25-97
COD Concentration and % Removal
AFFF Cone. = 60 ppm
CO D Standard Curve for High Range Vials
A B S O R B A N C E U N IT S (F O R 60 0 n m )
Reactor (A FFF ppm ) % COO Removal
A 1 (0 ) 9 2 .4
A 2 (0) 8 8 .6
A 3 (0) 96 2
% COD Removal
B1 (60) 23 .2
B2 (60) 20 0
B3 (60) 1 9 .3
R e a c to r (A FFF p p m )
i
File: A F F F Inhib (2 4 -1 1-97).xls Sheet: C O D
C-45
US00001170
A e , est: 3-25-97
COD Concenlration and % Removal
AFFF Cone. = 60 ppm
Fen ton s Pretreatm ent
F o r-A F F F ppm
480
480
480
____________________________ R e a c t o r 1
T a p w ater
1 2 5 0 ml
A ctu al A F F F ppm
1200
40 ml
H 2 0 2 , 3 0 0 0 -2 .5 m g/I Fe3+ 3 0 0 -2 .5 F e S 0 4 (3tim es)
7 5 0 0 m g/I 7 5 0 m g/I 3 7 2 3 2 8 m g/I
R eacto r 2
R e acto r 3
1250 ml
1250 ml
1200 40 ml
1200 40 ml
7 5 0 0 m g/I 7 5 0 m g/I
7 5 0 0 m g /l 7 5 0 m g/I
3 7 2 3 .2 8 m g/I 3 7 2 3 .2 8 m g/I
In itia l C O D A F F F -4 8 0 ppm
D ilution Factor A b so rb an c e
C O D m g/I
Actual C O D
33%
0 .3 3 4 |
4 3 8 8 .1
1 3 1 6 4 .2 7 $
C O D a fte r 24 hr re a c tio n p e rio d b e tw e e n fen to n s reag e n t an d A F FF
% C O D Rem oval
D ilution Factor A b so rb an c e
C O D m g/I
A ctual C O D
50%
0 .3 4 3 |'
4 5 0 6 .3
9012 668
3 1 .5 4
S a m p le C ontrols (A 1.A 2& A 3)
In itial CO D at Tim e 0 hr.
C o n stilu en t R R Decant
i
V o l (L)
! C O D m g /L
i 1
I 4 I 13 1 l
F eedstock AFFF T o ta l
! ___________
2
| 6
1037 9 O
2 0 7 5 .8 0 4 0
3 4 6 .0
In h ib itio n
RR Decant
(B 1 .B 2 S B 3 ) Feedstock
F en ton s treated A F F F
T o ta l
4
131
13.1
2
1037 9
2 0 7 5 .8 0 4
2
9012
18024
6 3 3 5 2 .2
File: A F F F Inhib (2 4 -1 1-97).xls Sheet. CO D
C-46
US00001171
jale of Tesi: 3-25-97
Oxygen Uplake
AFFF Cone. = 60 ppm
O xygen U plake during the Aerobic Stage
i i
A1 - -A 2 -A -- A3 - X -- B1
B2 - -- 83
I I
File: A FFF Inhib (2 4 -1 1-97).xls Sheet: Oxygen Uptake
C-47
US00001172
.e r est: 3-25-97
AFFF Conc. 60 ppm
S tage F e e d in g A n ae ro b ic A erobic
Dissolved Oxygen (mg/L) at various stages
A1 o .n f
A2 0 .0 9 1
A3 | 0 .0 9 ;
B1 ! 0.0 9 !
B2 0.0 8
0 071 5 581
0 ,0 8 | 5 .8 4 1
0 .0 9 i 6 .1 7 !
0 .0 9 j 6 .1 0 |
0 10 5.5 7
B3 0 10 0 .0 9 5 .8 5
S lag e A erobic
0| 30
_____________ I 90
120 150 180 210 240 270 ; 300 ] 330 ; 360 ; 390 ; 420! 4501 .480 510 540 570 600
8 06) 6 .1 4 ; 5 .7 o l 5 .3 6 j 5 .1 1 1 4 .8 2 I 455) 432! 4.0 7 j 3 .8 3 3 59; 3 .4 0 ; 3 201 3 03j 2.8 3 j 2 .6 6 j 2.51 j 2 .3 6 221 2.0 8 1 .9 4 j
D is s o lv e d O x y g e n in m g /L
880; 7 .2 1 636 5 91 5 .5 8 527 5 .0 1 4 .7 6 4 .5 1 1 4 .2 8 } 4 .0 7 3 851 3 .6 6 | 3 .4 7 1 3 ,3 0 | 3 .1 3 2 .97 2 .8 2 2 .6 6 2 .5 2 j 2 .3 7 j
899` 7 .8 8 7 .1 7 j 6.7 2 1 6 .3 9 } 6 .0 9 j 5 .7 9 5 .5 4 5 .2 7 j 5 .0 6 ! 4 .8 8 | 4 .6 4 j 4 .4 5 ) 4 .2 7 ! 4 09! 392| 3.7 7 3 .5 9 ) 3 .4 2 j 3 .2 9 .I 3 .1 4 j
9 .7 1 8 .27 7 .8 0 7.3 7 7 .0 1 6 .6 5 6 .3 1 6 01 5 .6 9 5 .4 2 5.1 5 4 .8 6 4.61 4,3 6 4 09 3.8 6 3.6 2 3 .3 8 3 .1 6 2 .9 6 2 .7 4
7 .8 2 7 .5 0 7.1 2 6.7 3 6.3 4 5 .9 8 5.6 3 5.3 0 4 97 4.6 6 4.3 6 . 4.0 8 3 .8 0 3 51 3 .32 2 .99 2 .7 2 2 47 2 .2 3 2 .0 0 1 .7 7
8 .7 8 8 08 7 ,6 3 7 .2 2 6 .8 4 6 .4 8 6 13 5 .8 0 5.4 9 5 .1 8 4 88 4.61 4 33 4.0 5 3 79 3 52 3 .27 3 03 2 .7 8 255 2.3 3
I
File: A F F F Inhib (24-11-97).xls Sheet: Dissolved Oxygen
C-48
i
US00001173
r
R e a c to r A1
A2 A3 B1
B2 B3
AFFF fppm )
H 202 m gfl
00
00 00
480
3000
480
3000
480
3000
Fe3+
m gfl
S ta g e
F e e d s to c k
RR Decant 0 E n d of Fee d in g
E n d of A naerobic
E n d of A ero bic
E x ten d ed A eration
E n d of S ettling 0 E n d of F ee d in g
E n d of A n aerobic
E n d of A ero bic
E n d of S ettling 0 E n d of F ee d in g
E n d of A naerobic
E n d of A erobic
300
E n d of S ettling E n d of Fee d in g
E n d of A n aerobic
E n d of A ero bic E x ten d ed A eration
E n d of S ettling
3 0 0 E n d of F eeding
E n d of A n aerobic
E n d of A ero bic E n d of S ettling
3 0 0 End of F eeding
End of A naerobic
End of A ero bic
End of Settling
Tim e >
2 hr 4 hr 6 hr (4 h r) 8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr (4 h r) 8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr 8 hr
TKN 3 9 .2 1 .5 1 5 .3 18.1 3 .7 07 2.1 14.4 1 5 .2 2 .4 5 .3 1 4 .4 1 5 .2 3 .9 5 .7 13 6 13.5 118 1 2 .5 90 128 13.5
125 16.1 136 152 11.1 1 2 .8
NH 3-N 23 .3 02 1 8 .3 20 .8 7.1 0 .2 0.1 1 7.6 2 0 .0 8 .3 5 .8 1 7.6 2 0 .0 9 .4 6 .2 15 .0 16 .4 14 .6 10.1 68 15 .0 1.6.4
14 6 9 .3
1 4 .4 158 . 151
9 .7
C o n c e n tra tio n , m g /L
O rg. N NCJ2-N N 0 3 -N
Total N
C l- P 0 4 -P
15.9 0 .0 0 .9 40.1 2 6 7 .5 1 1 .5 1.3 0 .0 3 .4 4 .8 2 3 4 .7 2.1
-3.1 0 .0 1.1 1 6 .3 2 4 2 .3 2 7 .4
-2 7 -3 .4 0 .5 2.1
0 .4 6 .5 2 5 .0 2 4 1 ,3 3 2 .8 4 .8 18.1 2 6 6 2 5 0 .8 6 3 0 .7 2 8 .8 3 0 .2 2 4 3 .0 3 .6 0 .0 3 0 .7 3 2 .8 2 4 6 .2 1.1
-3 2 -4 8 -5 9 -0 .4
0 .0 0 .9 1 5.3 2 4 0 5 3 4 .7 0 .0 0 .9 16.1 2 4 0 0 3 8 .8 5 .2 1 3 .5 21.1 2 4 7 .0 1 0 .2 4 .6 1 3 .9 2 3 .8 2 4 1 .6 4 .7
-3 .2 0 .0 0 .9 15.3 2 4 4 .7 3 2 .0
- 4 8 0 .0 1.1 1 6 .3 2 4 3 .7 3 5 .8
-5 .5 -0 6 -1 .4
4 .3
1 3 .0
2 1 .2 246.1
2 .5
4 .3 1 5 .0 2 4 .9 2 5 0 .4 3 .2
0 .0 0 .8 14.4 2 1 5 .8 4 7 .0
-2 .9 0 .0 0 .8 14.4 2 0 4 .2 5 2 .9
-2 .8 2 .4
0 .1
13 .0
2 4 .9 2 3 8 6 3 3 .7
0.1
16 .8
2 9 .4 2 1 5 .5 28.1
2 .3
0 .1
17 .0
26.1 2 0 9 .8 2 9 .3
-2 .2 0 0 0 .8 1 3 .6 2 1 1 .0 3 4 .4
-2 .9 0 .0 0 8 14 .4 2 0 9 6 24.1
-2 1
02
1 1.5
24 .2 2 1 3 9 2 3 .3
6.8 -0 8 -0 .6
0.1 00 00
1 1.2 09 0 .8
2 7 ,5 14 5 16.1
2 1 4 .2 2 1 2 .8 204 2
2 4 .6 38 .3 36 7
-4 ,0 . 3.1
02
12 .5
2 3 8 2 1 1 .2 1 9 7
03
14 .0
27.1 221.1 4 0 4
File: AFFF Inhib (24-11-97).xls Sheet: Concentrations Table
C-49
US00001174
R e a c to r
H 202 m g /l
Fe3+ m g /l
A1 0
0
A2 0
0
A3 0
0
B1
3000
300
B2
3000
300
B3
3000
300
AFFF
(ppm )
S ta g e
F e e d s to c k
R R Decant 0 E n d of Fee d in g
E n d of A n aerobic
E n d of A ero bic
E x ten d ed A eration
E nd of S ettling 0 E n d of Fee d in g
E n d of A n aerobic
E n d o f A e ro b ic
E n d of S ettling 0 E n d of Fee d in g
E n d of A n aerobic
E n d of A e ro b ic
E nd o f S e ttlin g
4 8 0 E n d of F ee d in g
E n d of A n aerobic
E n d of A ero bic
E x te n d e d A eration
E n d of S ettling
4 8 0 E n d of Fee d in g
E n d of A n aerobic
E n d of A ero bic
E n d of S ettling
4 8 0 E n d of F ee d in g
End. of Anaerobic
E n d of A ero bic
E n d of S ettling
- FS1 FS2
FS3
F S A varage
RRSU1
RRSU2
RRSU3
R R S U Avarage
Tim e
2 hr 4 hr 6 hr (4 h r) 8 hr 2 hr' 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr <4hr) 8 hr 2 hr 4 hr 6 hr 8 hr 2 hr 4 hr 6 hr 8 hr
C o n c e n tra tio n , m g /L
BOD
COD
TOC
1 0 3 7 .9 4 0 3 .3
13.1
1 2 .7
7 8 .8
1 5 .9
7 8 .8
1G .3
5 2 .6
14 .5
13.1
1 3 .7
2 6 .3
14 .6
2 2 3 .3
15 .5
5 2 .6
16 .3
5 2 .6
1 4 .1
3 9 .4
1 4 .1
1 0 5 .1 5 2 .6 13.1 13.1
16 .0 1 5 .7 1 3.9 1 3.9
3 0 4 8 .0 28509 2653 9 2 5 6 1 .9
8 5 4 .6 7 9 3 .3 8 0 7 .1 8 3 0 .8
2 5 7 5 .0 3 1 5 3 .1 2 8 7 7 .2 2759 0 2680 2 2 8 9 0 .4 2 8 9 0 .4 2896 0 2694 3
8 2 8 .2 7 8 6 .8 8 2 3 .7 7 9 7 .4 8 3 4 .5 8 0 2 .0 8 0 5 .4 7 8 7 .0 8 3 2 .8
3 95 .1 4 1 1 .0 4 0 3 .8 403 3
1 3 .5 12 .6 12.1 1 2 .7 1
| A lkalin ity H C 0 3 (m g /l) 5100 3000 5330 5 5 7 .0 3 7 1 .0 2 7 3 .0 2 6 7 .0 5 2 0 .0 5 4 7 .0 4 0 7 .0 3 6 7 .0 5270 5 2 1 .0 3 9 7 .0 3730 5770 5 4 8 .0 5 6 7 .0 5040 4 7 7 .0 593 0 564 0 5500 5 3 4 .0 577 0 542 0 555 620
File: A F F F Inhib (24-11-97).x!s Sheet: Concentrations Table
_ C-50
US00001175
Inhibition Effect of AFFF on Nitrification in terms of NH3-N
-- A1 -- -- A2 --A -- A3 -- -- B1 -- 3 IC -B 2 -- B3
T im e
Inhibition effect of A FFF on Nitrification as of TKN
-- A1 -- -A 2
-- B1 -- 3IC -B 2 -- C<-- B 3
File: A FFF Inhib (24-11-97).xls Sheet: N H 3-N & TKN Charts
C-51
US00001176
Inhibition effect of AFFF on Phosphorus removal
File: A F F F Inhib (24 -1 t-9 7 ).x Is Sheet: N H 3-N & T K N Charts
C-52
]
US00001177
Reactor A3 (No AFFF present)
S tage
File: A FFF Inhib (24-11-97).xls Sheet: Nitrogen Mass Balance
C-53
US00001178
File: AFFF Inhib (24-11-97).xls Sheet: Nitrogen M ass Balance
C-54
US00001179
R eacto r A1 (C ontrol)
AFFF ppm 0
H 202 m g /l 0
A2 (C ontrol)
0
A3 ' (C ontrol)
0
B1 4 8 0
0 0 3000
B2 480
3000
B3 480
3000
1 5 .0 0
12 0 0
E 9 .0 0
6 .0 0 X
3 .0 0
0 .0 0
e ia A1
m g /I 0
0 0 300
300 300
Fe3+ S tag e
N itro g e n C o n c e n tra tio n , m g /L
Tim e
N H 3-N N 0 3 --N
N 0 2 -N
(NOj--N)
End of F e e d in g
i 2 hr
18 34
1.1
0 .0
End of A n aerobic E n d of A e ro b ic
! 4 hr 1
! 6 hr
2083 7.0 9
6 .5 18.1
0 .4 4 .8
E x ten d ed A eration
(4h r)
0 .1 8
28 .8
0 .7
E n d of S e ttlin g
j 8 hr
0 .0 5
30 .7
0 .0 29.61
E n d of F e e d in g
i 2 hr
1 7 .6 2
0 ,9
0 .0
E n d of A n aerobic
i 4 hr
2 0 .0 2
0 .9
0 .0
E n d of A e ro b ic
6 hr
8 .3 2
1 3 .5
5 .2
E n d of S e ttling
8 hr
5 .7 5
13 .9
4 .6 12 95
E n d of F e e d in g
2 hr
1 7 .62
0 .9
0 .0
E n d of A n aerobic
4 hr
2 0.02
1.1
0 .0
E n d of A e ro b ic
6 hr
9 .3 8
1 3.0
4 .3
E n d of S ettling
8 hr
6.2 3
1 5.0
4 .3 1 4 1 5
E n d of F e e d in g
2 hr
15.0 0
0 .8
0 .0
E n d of A n aerobic
4 hr
1 6 .40
0 .8
0 .0
E n d of A e ro b ic
6 hr
14 55
1 3 .0
0 .1
E x te n d e d A eration
(4 h r)
1 0 .0 8
1 6 .8
0 .1
E n d of S ettling
8 hr
6.7 5
17 .0
0.1 1 6 .1 6
E n d of F e e d in g
2 hr
1 5 .00
0 .8
0 .0
E n d of A n aerobic
4 hr
1 6 .40
0 .8
0 .0
E n d of A e ro b ic E n d of S ettling
6 hr 8 hr
1 4 .5 5 930
115 1 1 .2
0 .2 0 1 10 62
E n d of F ee d in g
2 hr
14.41
09
0 .0
E n d of A naerobic
4 hr
1 5 .7 6
08
0 .0
E n d of A e ro b ic E n d of S ettling
6 hr 8 hr
1 5 .1 4 9 .6 8
1 2 .50 14.01
0 .2 0 0 .3 0
1 1 .6 3
A m m onia R em aining (Supernatent)
A2 f
5 75
|A3 1
6 73;'
HD
mj
30
B3 ;
lit (C o n tro l)
(C o n tro l)
Nitrate (Supernatant)
File: AFFF Inhib (24-11-97).xls Sheet: N 03- & N 02 Produced
055
US00001180
B N P Inh ib itio n T ests - 480 ppm AFFF Pretreated w ith Fenton's R eagent (Nov/24/97)
TSS R e a c to r A1 A2 A3 B1 B2 B3 TDS
R e a c to r A1 A2 A3 B1 B2 B3
TS R e a c to r A1 A2 A3 B1 B2 B3
Initial w t 1.0988 1.0995 1.0993 1.1159 1.1194 1.1116
Final w t 1.1388 1.1384 1.1381 1.1482
-1 .1 5 4 6 1.149
Initial w t 0.9889 1.0009 0.9991 1.0209 1 0178 1.0233
Final w t 1.005 1.017 1.0157 1.0734 1.0777 1.066
Initial w t 1.0086 1.0147 1.0096 1.0182 1.0234 1.0263
Final w t 1.0599 1.0656 1.0618 1.1181 1.1296 1.1265
V olum e 15 15 15 15 15 15
MLSS 2667 2593 2587 2153 2347 2493
Volum e 15 15 15 15 15 15
TDS 1073 1073 1107 3500 3993 2847
Volum e 15 15 15 15 15 15
TS 3420 3393 3480 6660 7080 6680
W T @ 550 C 1.1022 1.1022 1.1024 1.1193 1.1235 1.1152
MLVSS 2440 2413 2380 1927 2073 2253
2TSS.TD S 3740 3667 3693 5653 6340 5340
File: AFFF Soilds D ata
C-56
US00001181
APPENDIX D -l BNR Inhibition Pass-Through Acute Toxicity Test Results
60 ppm A FFF Pretreated with D efoam er 8710
D-l US00001182
e
D-2 US00001183
Coastal Bioanalysts Inc.
O D U /O ceana Acute Effluent Toxicity Tests
Mysidopsis bahia & Cyprinodon variegatus
Sample I D. Feed Contract No. N00187-93-D-9748
i Delivery Order No. 0235 J O. No. 1912290, Item No. 0002BJ, 0002BH, 0003AD
PWCNo. 1351 9/28/97 2300 Sample No. JRA97-10760
Submitted to: Ms. Merrill Anderson-Asheraft
Navy Public Works Center Code 930, Building Z-140
Norfolk, VA 23511
9
By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062
(804) 642-0168
D-3
US00001184
O D I'.O C E A N A Sample t.D. Food 9/2X.97 Sample 97-10760 Test ID: JR-O7109
Coastal Bioanalysts Inc.
I. TEST RESULTS
% Effluent Concentration
0 6.3 12.5 25.0 50.0 100
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acute Test Results
Mean (%) Organism Survival
M . b a h ia
2iLh 100 85 20 0 0 0
48-h 100 80 0 0 0 0
C v arieg atu s
2 48-h 100 100 55 30
55 20 5
50 00
8.1% N/A Trimmed Spearman-Karber
12.35
3.5% 0.19-6.04
Probit 28.57
Acute Test Information:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/har/est Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
A/. bah ia Test 48-h Static Survival 9/30/97 1045 10/2/97 1115 CBI stock cultures 9/30/97 20 g/kg 19.5-20.7C
No N/A
C. variegatus Test
48-h Static Survival 9/30/97 1050 10/2/97 1100 CBI stock cultures 9/30/97 20 g/kg 19.5-20.7C
No N/A
Although D O. concentration decreased to less than 4.0 mg/1 in higher effluent c o n c e n t r a t t o n s the d op v as
u n a n S a t e d and probably caused by a bacterial bloom (solutions turned milky color ovem.ghO. L o ^ n 0 , was not
primary cause of toxicity as indicated by low survival in concentrations with adequate D.O. concentration.
DU
US00001185
ODU/OCEANA Sample I D. Feed 9/2S/97 Sample 97-10760 Test ID: JRA 97I09
Coastal Bioanalysts Inc.
Sample Collection Pate/Time
9/28/97 2300
Sample Use Pates/Times
9/30/97 1045/10503 ,
Aeration2 Pate/Time
n/a
Pretreatment/ Adjustment
HWM Salts
2Aeration often necessary to reduce sample oxygen concentration to 100% saturation after warming. bahia/C. variegatus test time.
Table 2. Effluent and Dilution Water 4 Chemical & Physical Characteristics (Mean Value (Standard Deviation))
Parameter
Temperature (C)
Salinity (g/kg)
Conductivity (uMHOS/cm)
pH
Dissolved Oxygen (mg/1)
Total Hardness (mg/1 as CaCOj)
Alkalinity (mg/1 as C aC 03)
Total Chlorine (mg/1)
n h 3-n (mg/1)
Initial Effluent
4.2
(--)
N/A
2080
(-)
7.01
(-)
11.2
(-)
129
(-)
399
(-)
<D.L.
(-)
36
(-)
Adjusted Effluent 20.9 (-) 20 (-) N/A
7.09 (-) 7.9 (-) N/A g N/A
N.D.
N.D.
Dilution W ater 20.8 (-) 20 (-) N/A
7.90 (-) 8.0 (-) N/A
N/A
N.D.
N.D.
Effluent parameters measured upon receipt of sample in lab and after adjustment on day of use^ ^ l'U^f ^ ^ ater ^
w-Marincmix in ASTM Type I deionized water) values measured after preparation of diluent o y
-
D-5 US00001186
ODU/OCEANA Sample I.D. Feed 9/28/97 Sample 97-10760 Test ID: JRA97109
Coastal Bioanalysts Inc.
H. WATER QUALITY
Earametec
Temperature (C)
DO. (mg/1)
pH
Salinity (gAg)
Control
20.6 (0.3)
7.8 ' (0.3)
7.71 (0.17)
20 (0)
Table 3. W a te r Quality Summary
M . bahia Test
Mean Value (Standard Deviation)
6 3%
9
20.6 (0.3)
6.4 (1.6)
7.61 (0.15)
20 (0)
12.3%
20.6 (0.2)
6.2 (1.6)
7.66 (0.16)
20 (0)
25%
20.9 (0.1)
5.4 (3.6)
7.73 (0.39)
20 (0)
50%
20.9 (0.1)
5.0 (4.1)
7.64 (0.55)
20 (0)
100%
20.9 (0.1)
4.6 (4.7)
7.58 (0.69)
20 (0)
Parameter
Temperature <C)
DO. (mg/1)
pH
Salinity (gAg)
Control
20.4 (0.6)
7.8 (0.2)
7.84 (0.09)
20 (0)
C. v a r ie g a tu s Test
Mean Value (Standard Deviation)
6 3%
20.4 (0.6)
6.3 (1.8)
7.55 (0.20)
20 (0)
12.5%
20.4 (0.5)
6.3 (1.7)
7.57 (0.16)
20 (0)
25%
20.4 (0.5)
5.3 (3-D
7.53 (0.15)
20 (0)
50%
20.5 (0.5)
4.5 (3.2)
7.60 (0.34)
20 (0)
100%
20.8 (0.1)
.5.2 (3.9)
7.45 (0.50)
20 (0)
D-6 US00001187
O D I'''OCEANA Sample I.D. Feed 9/28/97 Sample 97-10760 Test ID: JRA97109
m . QUALITY ASSURANCE
M. bahia - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
C variegatus - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
Coastal Bioanalysts Inc.
CBI Stock Cultures 9/16/97-9/18/97 KCl (Sigma "Ultra" Lot#76H10391) 343, 490, 700, 1000 mg/1 100% 651.8 mg/1 (605.9-701.2) 611.0 mg/1 (483.0-739.0)
CBI Stock Cultures 9/16/97-9/18/97 KC1 (Sigma "Ultra" Lot#76H10391) 432, 720, 1200, 2000 mg/1 100% 1174.3 mg/1 (1048.0-1315.9) 1063.1 mg/1 (901.6-1224.5)
B\
D-7
US00001188
(J
CUMULATIVE DATA SUMMARY NPDES Permit #: N/A Oceana - ODU Feed S.
D a te o i'i. IvriBrat^i-:'.;:.r - Vertebrate;'5^7Tr~ ''; lavertebrate " "
2/12/97 <6.25 2/28/97 Invalid 3/12/97 17.7 3/20/97 19.5 3/26/97 33 9/30/97 8.1
31 35 . 52 35 34 3.5
Vertebrate- ' ; 7 NEC %i=. ,
Supplement D-8
US00001189
COASTAL BIOANALYSTS. IN C NPDfcS STATIC ACUTE; SALTWATER
(Form AMU-#)
% E ffluent C ontrol
1.0 .
C-l C-2
O ayl f liv e
O
li)
1-1
{6 .2 5
1-2
12-1
O1 2 .5
22
3-1 o
O2 5 3 -2 o4-1
5 0 4-2 c
o5 - 1
100 5-2 c ,
O bserver Tune:
I3K
0*y 2 1 Lrve
16 _____________
______________
____________
O -6
r.
_ _ ____________
0 o
o
( .-I T
Final \
Survival
/C o
$0 0 0 0
M v s id o o s is b a h a
Source:
hn
C 8I
--i.
QH W M A S W
O th ar
S a lin ity :
to e?0 ' l
F w d n o onor to te st:
TA n e m ia - w
l O th e r
rinrjrfctfingtesr Not fed D \XA n im a /d Qthar-
C h a n te r* -
S3 0 0 m l
5 0 0 m l
1 0 0 0 m l
O thar:
TlItlT,l,,_. 2 0 0 ml r a
2 5 0 min
5 0 0 i d
O tte r
-- id
irt
3P t k itu f M io d 1 6 L :8 0
O th er
STUP- D a n
T j 1 ?
Im a A rarais A d te i
No. ro e caiei: r O
f ta n t to m z a ti o n f e r m i a t e n o .
Tuna W aiar A d ted
/ "3
Sal U p By_f G___
IN S rR U M tN IA F IO N USED:
4t u m T h a n n i m a i a r N u t t e r
|
Q rao.o.m i (sn80m<t5|
53 C P O i t a ' s * pH M a ta r ( 0 9 5 0 0 5 6 3 )
Sartonus RI 6 0 P B alana (4 0 0 2 0 0 9 3 )
y S SCT 33 M atar {H 6016792I
EJ Y S 0 . 0 . M a l a t i 2 ( S N 9 1 A 0 2 6 3 2 9 ) C o n an g 2 4 0 pH M a ta r |S > 6 2 6 8 )
S P P o m p H 2 M atar 18523601
^ Spartan ATC R e fra cta rre ter (A 366)
0Y 0
O ar
0y 2
C &0 ^
.?*. )
T 1 ? 0 7
2 2 c .n
7.
M 3
.TV ?
--
*3 a 00 e! 5 9 * c( zx> i
-- --
C "7 - to
7 :0 ?
1 7 " 7 '
77? 7-S ?-
2 V -l ^ 7 7 /
7 -.V -
pH 3 1. v 7
--
4 ~?
?03 --
5 V - Li % -Ok --
C 3 o 7,-N . r S I _
1 % 0 C.
0-0. Irrrj'D
2
3
4
5
sC
A1 L
2
N3
4
5
tR e p lic a t e
Imitais:
7? "7 7
7 .? 7- ? 7? :> Pi
9
u. 0
O -g O .i
/ '
J20 OO
S>0
rPO
-T O 77
/ .O~
Y -? -- -- -- --
____
-- -- -- _ i _____ f,- t f _ _
MOTFS
rf 'n > f y c ^ " - ' * > [ ?
Qh I C>tG---------------------
Q uality A s s u m e :
A s Q uality A s s u m e o ffice r for t h j t st I certify th a t no sig n ilea n t A v ia tio n s fro m a c c e p te d p ro to co l c u r e d tfcnnq routnw . e tio n s (u ilM S o th e rw is e n o te d below } and th a t th e a tta acc u ra tely relU c t th e te s t a s it w a s p e fto rm td b y CSl.
C o m w in .L ~ T ) O
A m - 1 < * - * < * ' / - P - ^ ` 7 -
( t ^ I < 7 -. */* *-< g i V ^ t ^ ^
T EST ID: J / l / / ^7/O .Z
D-9
US00001190
TRIMMED SPEARMAN--KARBER METHOD. V E R S I O N l . S
DATE: 9/30/97 TOXICANT : FEED #13
SPECIES: M BAHIA
RAW DATA :
Concentration
(%) .00
6.25 12.50 25.00 50.00 100.00
TEST NUMBER: JRA97109
DURATION:
Number Exposed
20 20 20 20 20 20
Mortalities
0 4 20 20 20 20
SPEARMAN-KARBER TRIM:
2 0 .0 0 %
SPEARMAN-KARBER ESTIMATES:
LC50:
8-11 95% CONFIDENCE LIMITS
ARE NOT RELIABLE.
48 H
D-10
US00001191
C O A S T ,\ J. H IO A N A I.V S T S . IN C .
N P D K .S S T A T IC A C I rT F v S A i.T W A T F .R
(Form ACV-IX)
\ E ffluent
1.0 .
Oay t A liv e
C o n tro l
C -l C-2
6 .2 S
M 1-2
1 2 .5
21 2-2
3-1 25
3-2 4.1 50 4-2
5-1 100 S-2
O tse rv e r T im e:
IO
1
lo I a
3 7
i
o
c 0
C~H fZK
On 2
1 l/v e
1o IO 1
_ 5 I ___________ 1
3
C> 1
O___________
_ Q ____________
_ _______________
rs "
fr-H 1(00
final \
Survival
ICO
3o 5 5
0
s a :
C y p r i n o d o n v a r i e q a t u s __________
Some:
CB!
H atch O ate/T im e:
9 / 3 d/? 7
Acdirotion w ater HWMASW
Other
Salinity:, P c )
Acclirration Terr, frange):
i 9 _______ 10
F eed n g pnor to feat:
A rtem a
)(
/ d O t h e r ________________
f e d r q <rrrq t e s t : N o t f e d Q
A rtem e
/d O th e r_
Omnerm 3 0 0 H
5 0 0 fri
1 0 0 0 f ri| I
O t h e r ______________ <ri
r * TM
200* Q
250*
so o * j a
tr a t ta i 1 . o n j e m v r a p e r f w l i c m :
f ___Q f*-
O th er
fri
--
P h o to p eo o d 16 1 :8 0
O th er
R endo rn g en o n t i r a t e n o .* ^ ~ A
SETU P: D ete_ 9 / 3 o / y * 7
T irm W ater A dded
/O ^ S
T in A n rra fs A d d
/ O 5
S a t U p By
--
INSTRUMENTATION USEO:
S tem T herm om eter N u n te r Y S 0 .0 /M e te r * t (S N E 804475) ^ C P D tgraense pH M e ter (0 9 5 0 0 5 6 3 ) S erto m o R 160P W a re (40020093)
U YSI SCT 3 3 M ater (H 8016 7 9 2 )
Q V S 0 .0 . M eter 2 (SN 9t A 026329) f i Corrang 2 4 0 pH M e ter (S N 5268)
D SP P o r u p M eter (8S23S0) CBS e e n A T C R e f r a c tn m e te r (A 3 6 6 )
Oey0 0ay I Cay2
C
T1
E2
M 3
P
4
5 c
1
2
pH
3
D .0.
(mail)
5 c
1
2
3
4
5
sC
A1 l
2
N3
4
5 9Replicate
Initials:
<?o% 0 0 .7
.It t 06:-? sto 2
(7.1
/f.s
ns
3. C
7dfc
' 70S
117 St.C,
9HP\
--
1 .7 o
7 - gl
f 79 r
7 -?i 7 ? 7 . . .
lA r'\ 7 . 7 *
7 Ub 7 . 0
Do
l o o 7. ii 1
7 .0 ?
7 - S --
VO
7 7 ... 7 - . U
C -o
3
V. <
19 o v
1-1*
i ci 7 e!
'0
1 ,4 1, Os
79
Q .v
--
7! ,,20
.A-0 Pi po
P t i _______
3 k ______
D * P 0 6
DO Pz
3 ii
=30
P?
--
Q
P -&
iA
(D
. J 2 ______ G9
NOTES: Sei--.j^y/4 -0 " V rJ - - t- I t ; ' C f*'..
b>Y
1____ [ d l -----------------------:-------------------
QualityAjtmnce: -
As Q u riity A i t i n r c i o fficer for ifw te s t I c ertify th e t no significant d M ia tm s frw i a c c e p te d protocol o ccu rred dunng routine in sp ectio n s ( i r t e u o th e rw is e n o te d below ) and th a t th e ctota a cc u ra tely refle ct th e te s t a s it w as perfo rm ed by C8I.
d > K .., ,
D -ll
T E S T I D : ~J 0 ^ 1 ^ \
US00001192
EPA PROBIT ANALYSIS PROGRAM USED FOR CALCULATING LC/EC VALUES
Version 1.5
JRA97109 ACV FEED #13
Cone.
Number Exposed
6.2500 12.5000 25.0000 50.0000 100.0000
20 20 20 20 20
Number Resp.
14 19 19 20 20
Observed Proportion Responding
0.7000 0.9500 0.9500 1.0000 * 1.0000
Proportion Responding Adjusted for
Controls
0.7000 0.9500 0.9500 1.0000 1.0000
Chi - Square for Heterogeneity (calculated) Chi - Square for Heterogeneity
(tabular value at 0.05 level)
= 1.296 = 7.815
JRA97109 ACV FEED #13
Estimated LC/EC Values and Confidence Limits
Point
Exposure Cone.
95% Confidence Limits
Lower
Upper
LC/EC 1.00 LC/EC 50.00
0.346 3.467
0.000 0.192
1.472 6.041
a
D-12
US00001193
COASTAL bio a n a lysts INC
EFFLITENT/DILUTION W A T E R DATA (Form truum)
INITL\L (ARRIVAL) EFFLUENT CHARACTERISTICS:
Sample Date l^ f r/^ T l '
1
1
II
Temp (C) w . a i
Conduct. (uMHOS) Ic^O^O l
i i II ilil
I
Salinity (g/kg) I M
1
1
1
I
I
I
pH I7-./ I . i i I I I
Diss. Oxygen (mg/l) 1 H . Q 1 1 1 I I I
Tot. Chlorine (mg/l) l'->c 1
I .1
I
I
I
NH3-N (mg/l) 1 3 0 1 1 1 I I I
Hard (mg/l CaC03)
11I I I
Alkln (mg/l CaC03) \ Z 1 < \ I I I I I I
I I IColor/Appearance1 i c y
i
i
i
Initials
(r If I ' I
I I I I
1 C-Clear O-Opaque Y-Yellow B-Brown BI-BIack G-Green T-Turbid SI -Solids (S-Slight M-Moderaie V-Very Much)
EFFLUENT ADJUSTMENT/POST-ADJUSTMENT MEASUREMENTS:
Date h/io/H
I li
Temp. (C)
I I 11
ISalinity (g/kg)
2
I
I
11
D O. (mg/l) r - M i i l i
Aeration Rate I jDr I I l i I IAeration Time (min) A//) I I l
Adjust. D O (mg/l) I ~ I I 1 1
pH I 709 I I 1 1
ITot Chlorine (mg/l) h a !
I li
Initials
I Po I
I
li
l
l
l
l l
i I 1
i
i
1 1 1
i i
I 1
1 1 1
ACUTE
II II II II II
li
II II II II
DILUTION W ATER CHARACTERISTICS:
Date
\ f f ' lrn I
I
Temp. (C)
II
I IConduct (uMHOS) a/A
I
ISalinity (g/kg)
To
I
I
PH
I7.70 I
I
IDiss. Oxygen (mg/l) 0 I
I
IHard (mg/l CaC03) ! p \
I
IAlkln (mg/l CaC03) /U/T
I
Initials
Ifb I
I
DMW:
!
l l l 1 1 1 l l
HWM:
1*1 ili ili ili
1 1' 1I 11 ili ili
1
1 I
l-
ACUTE
II II li li II l II Il ' II
EFFLUENT ID:
,0.
3tf-
*"1
TEST ID: T 7. -iCf H + \ oC\ D-13
_
US00001194
CHA1N-0F-CUST0DY RECORD
& ANALYSIS REQ UEST FORM
C LIENT INFORM ATION CQMPANY/OMMANP: (P .Q lA
UJRNAftQUMO (O iyO
(FOR RUSH TURNAROUND STATS REASON BELOW)
C O M M E N T S :i;
Jrt U w Q c 7 -- - U .
jjlh u x p h .~j~.J-- ffF - .0=.Q
^ J -A rju j^
_____j_ il\ i \\ yv /A ^ . rc^ a7&r s (l sV -(P l---------------------------- ,, S A M P L E D IS P O S A L : ( (R E T U R N T O C L IE N T ( (D IS P O S A L B Y L A 8
R E G U L A T IO N A P P L IE D :
RCRA (
) . HRSD ( )
SOW A (
) TSCA ( )
CW A (
) PHOTO ( )
'C A A
()
O THER( ) ---------- ;------------ ---
^ ^^
^ ` C-
IU Jf^ A i i i . " y ' . i s / i
7
c w r o / w f f l , 5 r e s p o n s ib l e f o r a l l c h a r g e s n e c e s s a r y f o r THE PROCESSING a n d AN A LY S IS o f s a M l e is i. -
S A M P IIN G /C O U E C T IO N CHARGE:
P O S S IB L E S A M P L E H A Z A R D S:
COMMENTS: '
AutvalTemp,
O ta *
n.O. NUMLU:
IN ir.:
C O N T R A C T L A B : _____________________ D A T E : '
C O N T R A C T N O .(S ): -QFnUACVSEVNVE'
ALL RUSH SA M PLES A R E S U B JEC T TO SU RC H A RG E.
. r r r n J I V ,, n T T T X i----- n ~ io PM !
2 A . P , , , P F c n VFO AFTER 7 :0 0 PM M O N THURS. AN D 1:00 PM ON FRIDA Y WILL BE PROCESSED THE NEXT BUSINESS BA Y (T.-QO A M - 3 .3 0 P J ^
*" "
v ii
SAMPLES VERIFICATION
REJEC TED ! )
R E A S O N : H O L D IN G T IM E ! )
C O N T A IN E R S ! )
IM P R (?P E R LY S A M P L E D ! )
OTHER( _ L
t ^p l i n u u i s h l U
relinquished
CHAIN-OF-CUSTODY RECORD
E N V IR O N M E N T A L
& ANALYSIS REQ UEST FORM
PW C EN VIRON M EN TAL LABORA TORY C O D E 9 3 0 B LD G Z - 140 9742 MARYLAND AVENUE NORFOLK, VA 2 3 5 1 1 -3 0 9 5 PH: (7571445-8851 FA X: (7571445-8852
CLIENT IN FO R M ATIO N
COMPANY/COMMAND: OT)U /f ) C j o.,^ CODE:
CONTACT: ft ' O i r X r . ___ "_________
PHONE: 6-S.WC/.V?-<w XT:
FAX:
JO. #: 1 iU \iF
SIGNATURE: C O h ! 1 h?
P E R M IT N O
T iirS 'A C rC M S 0 : n l : COM M ENT?:
(TO R RU SH TURNAROUND S TATE REASON UELOYY)
R E G U L A T IO N A P P L IE D :
RCRA ( )
HRSD ( )
SDW A ( )
TSCA ( )
CW A ( )
PHOTO ( )
CAA
()
O THER ( )
S A f.'.P U .'.'G /C O U E C T IO R C H A R G E: P O S SIB LE SA M P LE H A Z A R D S : COM M ENTS:
. A M P L I; D IS P O S A L : ( (R E T U R N T O C L IE N T ( (D IS P O S A L B Y LAO
-vat* '* c U
Y lT T M T : ' C O N T R A C T L A B :"
I. CUSTOMER IS RESPONSIBLE TOR A U CHARGES NECESSARY FOR THE PROCESSING n O ^ N ^ S i T o f I ^ P ^ I S ^
A LL R U SH SA M PU F.S A R E S U O JE C T TO S U R C H A R G E .
'v'H t- R
C O N T R A C T N O .(S ):
XKXOBT9
V F F IC K C V S E 137R rr~
- - ^ * Tl n. 2 --- --- ' M 0 / '>' " T,<uns - AKO JJ)OPA1_ON_ERIOA Y WILL RE PROCESSED THE N E X T BUSINESS OA Y (7 :0 0 A M . . 3 :3 0 P M I
! - p o d l a d u s e O N LY !!
s a m p l c s T 7 eT 1 f i c a t ci'n
-.-TM >
~ "" =
(R E J E C T E D ( _) ( R E A S O N : H O L D IN G T IM E ( )
:T?STrrTK0TsTfH^jT
j/t - 1 /
C O N T A IN E R S ! )
) t
O T /V ., 1 -,,
I H E C T Y . ' / p ^ , - , //,_ c,(t
r / ^ I t 5 - v /l j u
jx . 0 b v
CQ M PAN Y/QM M ANQ: a ) f -
`I ^ V
0 T E ? r M I / /L o o
D-16
i
US00001197
Coastal Bioanalysts Inc.
O D U /O ceana Acute Effluent Toxicity Tests
Mysidopsis bahia & Cyprinodon variegatus
Sample I.D. Mixed Lq Contract No. N00187-93-D-9748
Delivery Order No. 0235 J.O. No. 1912290, Item No. 0002BJ, 0002BH, 0003AD
* PWCNo. 1352 9/28/97 2400 Sample No. JRA97-10761
Submitted to: Ms. Merrill Anderson-Ashcraft
Navy Public Works Center Code 930, Building Z-140
Norfolk, VA 23511
9
By: ' Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062
(804) 642-0168
D-17
US00001198
ODU'OCEANA Sample I.D. Mixed Lq 9/28/97 Sample 97-10761 Tesi ID: JRA97110
Coastal ioanalysts Inc.
I. TEST RESULTS
% Effluent Concentration
0
6.3
12.5
25.0
50.0 100
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acute Test Results
Mean (%) Organism Survival
M . bahia
C. variegatus
24-h
48-h
2 4gJi
100 100
100 100
100 100
100 100
95 95
100 100
100 100 100 95 100 95
100 100 100 100 100 100
>100% N/A N/A <1.0
>100% N/A N/A <1.0
Acute Test Information:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
M . bahia Test 48-h Static Survival 9/30/97 1050 10/2/97 1125 CBI stock Cultures 9/30/97 20 g/kg 19.5-20.7C No N/A
*
C variegatus Test 48-h Static Survival 9/30/97 1055 10/2/97 1135 CBI stock cultures 9/30/97 20 g/kg 19.5-20.7C No N/A "
Aeration necessary when effluent oxygen demand results in dissolved oxygen concentration < 4.0 mg/1.
D-18
US00001199
ODLVOCEa NA Sample I.D. Mixed Lq 9/28/97 Sample 97-10761 Test ID: JR A 97110
Coastal Bioanalysts Inc.
Sample Collection Date/Time
9/28/97 2400
Sample Use Dates/Times
9/30/97 1050/10553
Aeration2 Rate/Time
n/a
Pretreatment/ Adjustment
HWM Salts
2Aeration often necessary to reduce sample oxygen concentration to 100% saturation after wanning. 3A/. bahia/C. variegatus test time.
Table 2. Effluent and Dilution Water 4 Chemical & Physical Characteristics (Mean Value (Standard Deviation))
Parameter
Temperature (C)
Salinity (g/kg)
Conductivity (uMHOS/cm)
pH
Dissolved Oxygen (mg/1)
Total Hardness (mg/1 as CaCO j)
Alkalinity (mg/1 as CaCO j)
Total Chlorine (mg/1)
n h 3-n (mg/1)
Initial Effluent
5.1
(-)
N/A
1517
(-)
8.03
(-)
11.6
(-)
114
(-)
266
(--)
<D.L.
(-)
2.1
(--)
Adjusted Effluent
20.9
(-)
21
(--)
N/A
.
Dilution W ater
20.8
(--)
20
(-)
N/A
7.97 7.90
(-) (-)
8.0 7.9
(_) (--)
N/A N/A
N/A N/A
N.D.
N.D.
N.D.
N.D.
4Effluent parameters measured upon receipt of sample in lab and after adjustment on day of use. Dilution water (Wimex hw-Marinemix in ASTM Type I deionized water) values measured after preparation of diluent on day of use.
D-19
US00001200
ODU'OCEANA Sample I.D. Mixed Lq 9/28'97 Sample 97-10761 Test ID: JRA971 IO
Coastal Bioanalysts Inc.
H, WATER QUALITY
Parameter
Temperature (C)
D.O. (mg/1)
pH
Salinity (g/kg)
Control
20.6 (0.2)
7.8 (0.1)
7.78 (0.11)
20 (0)
Table 3 Water Quality Summary
M . bahia Test
Mean Value (Standard Deviation)
6 3%
2 0 ?7 (0.2)
7.7 (0.2)
7.87 (0.14)
20 (0)
12i%
20.6 (0.3)
7.7 (0.3)
7.89 (0.12)
20 (0)
25%
20.6 (0.2)
7.7 (0.4)
8.00 (0.09)
20 (0)
50%
20.5 (0.2)
7.7 (0.3)
8.08 (0.08)
21 (0)
100%
20.6 (0.3)
7.7 (0.5)
8.18 (0.19)
21 (0)
Parameter
Temperature (C)
D.O. (mg/I)
.
pH
Salinitv (g/kg)
Control
20.4 (0.6)
7.7 (0.2)
' 7.78 (0.19)
20 (0)
C. variegatus Test
Mean Value (Standard Deviation)
6.3%
20.4 (0.6)
7.7 (0-3)
7.85 (0.25)
12.?%
20.5 (0.5)
7.6 (0.4)
7.88 (0.21).
25%
20.4 (0.5)
7.6 (0.4)
7.96 (0.13)
50%
20.4 (0.4)
7.6 (0.5)
8.06 (0.07)
20 20 20 21 (0) (0) (0) (0)
-
100%
20.5 (0.3)
7.6 (0.7)
8.14 (0.15)
21 (0)
D-20
US00001201
ODU'OCEANA Sample I.D. Mixed Lq 9/23/97 Sample 97-10761 Test ID: JRA 97110
m . QUALITY ASSURANCE
M bah ia - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
C variegatu s - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
Coastal Bioanalysts Inc.
CBI Stock Cultures 9/16/97-9/18/97 KCl (Sigma "Ultra" Lot#76H10391) 343,490, 700, 1000 mg/1 100% 651.8 mg/1 (605.9-701.2) 611.0 mg/1 (483.0-739.0)
CBI Stock Cultures 9/16/97-9/18/97 KC1 (Sigma "Ultra" Lot#76H 10391) 432, 720, 1200, 2000 mg/1
100%
1174.3 mg/1 (1048.0-1315.9) 1063.1 mg/1 (901.6-1224.5)
D-21
US00001202
CUMULATIVE DATA SUMMARY NPDES Permit #: N /A Oceana - ODU M ix L. R.R.
Date,of- _Invertebrate: - -
tSsMifWt-warnTest^-x>^
Vertebrates
s s .~
2/12/97 >100
>100
oO
A
2/28/97 Invalid
3/12/97 >100
>100
3/20/97 >100
>100
3/26/97 >100
>100
9/30/97 >100
>100
Invetbrati-
Vertebrate. - - A--
TOECl5^ i!r~--tt
S u p p le m e n t
D-22
US00001203
Co.-WYAI. UIO.VNAJ.YSTS. INC NPDES STATIC ACUTE/SALTWATER
(Form AM 1348)
X Eifluerit
Oay 1
1.0 .
1 Lnre
Day 2
fL o r e
C o n tro l
6.25 .
c-l
C-2
1-1
1-2
16
id
! >0
I C) (0
>0 /
125
2-1 22
3-1 25
3 -2 4-1 SO 4-2
100
5-1 S-2 0 lA *rvw f!
lb ID I ID
I6
ID I Cr- f-f
_ 2 __________ _ j __________
. Jo
(0
_____________
9 <
Tim e:
U Q ______
uV
cr
*F in al
Survival
|C D
jOG
ico
M v sid o o sis b a h ia
S o u rc e:
C 8I
A cd in au o n w ater HWM ASW
O th er
S * ln u rv :2 i C )
A rrlim ahon lem o. (ranoel:
^
F v e c k n j f*> to t e s t :
A n a n * ^ )(
U K p O .^ /d O th er
fe e n g dunny te st: N ot fed ^ 3
A r t e r r u l _____ /<*
<h^
C h a rt /w a : 3 0 0 r t Q
S O O rtQ
1000 ml ^ 3
O th e r
id
T astvolum a: 2 0 0 r t ^
2 S 0 rtQ
5 0 0 frt n
O th e r
id
f k reolicates: c p ' / , 3 -
Photooenod 1 6 l:B 0 |J 2
O th er
R an to rm an o n tem etele n o .* ^ " / j ^ .
H ff 7" ( Y &S E T U P D a
I2>/
T u r W a te r Atfcfcd
Tim A ra m e li A d d ( ^
S m U o Bv
INSTRUM ENTATION USED:
S t e m T h e r m o m e te r N u r r t e r {{ ] _ _______
O YSJ 0 . 0 . M m 1 1 ( S N E B O H I T S ) f v 3 C P D >g>wrse pH M e te r ( 0 3 5 0 0 5 6 3 ) San o n u s RI 6 0 P B aian e. (< 00200931 1~1 r a SC I 33 M alar IH 8 0 IS 7 9 2 )
E ] Y S 0 .0 . M alar 1 2 (SN 91A Q 2S329) L 3 C om ing 2 4 0 pH M e te r ($M > 268) SP P o r t M a i (8S2360) ( P Spartan ATC R a /ia clo rralar IA 366)
C
T1 2 M
p3 4
5
C
2
pH 3
4
5
C
l 0.0. 2
(m gft)
3
4
5
SC
Al
L
2
N3
4
5 R eplicate 9
Im tials:
Oay0
X idX Xl> X Xb . j
Oay 1
Ji A
9<-J
D ay?
D t.y
0c.<;
la 7 d .-
n C .5
76 9
^D . f
oT - . 9 T d3
y^ < 5 1. 7 6 T ti
A d9 0> f
S .O
7 -S 5
7 .5 fo
S'. 7 . 1 7
S' . A
? 7-f
c -l5 <?-?$
?. A ? ?. 7
T --7
T -.f
7- f T-.t
>%
2 -5 T ^v
f.7 )
i :7 a mD 3 2) 7A CM 7) / /
..a :-
7 -7 .,_ 76
76
16
A -o
76
>1 A l -- M<5M
r)
P -- C'ff
NOTES
Q iab ty A ssu m e : A s Qualify A s s u m e o f f i c e r f o r th is t e s t i c e r t i f y t h a t n o s ig ie f ic a n t (tax a tio n s from a c c e p te d p ro to co l o ccu rred d jn n g routine inspections j i r i a u o th erw ise n o te d below ) a n d (h at th e d a ta accu rately reflect the te s t a s it w a s perform ed by CS1.
C om m ents
-
TEST I D :
-^ T l
D -2 3
/
US00001204
COASTAL UrOANALVSTS. INC. NPDFS STATIC ACl/TE/SALTWATER
(Form ACV4X)
%E f flu e n t
1O ey
1 .0 . Live
C-I ID C-2 1 D
M '
12N6 .7 5
1-2
2-1 1 0
I D1 2 .5
2-2
I d3-1 D2 5
13 - 2
-1 i D
50
4-2 f as
51 i D 1fc1 0 0
52
O bserver
GH
Tim a: M I M ___
fO ey 2 liv e
I
IA
If
id
id (o
10
'
0
l
l l
C-P 1135"
%F in al
S u rv iv a l
i CO
1oo
1Oc 100 1c 0 |C0
S o eciw :
C y p r i n o d o n v a r i e q a t u s __________________ S o u r o t: _
Hatch DateTime:
/ A C\ f
________
CBI
Acctirration w a te r HWM ASW { 3
Other
. Salinity J f l
n Tem p- fraoge): Feeing prior to test: Artema ^ ^
__ to D o ; r O tf r_ _ _ _ _ _ _
Feeing during test: Not fed 0
Artema
. / d Other
0 r t r . :
300^n
500*
1000*0
O th e r_
7 . ! TM : 200 *
250 *
In tia l no. orgaresrra per rep licate:
fQ
500* P
O th a r._
Pfa. r e p lic a ta :
ftio to p en o d 16L 0D
O th er
R andom ization le m p la ta no. ^
SET UP: D ate
f i 6 1 ^ 1 - _ T u r n W a t e r A d i a d { M u _________
Tim e A n m als A d d ed f D S ~ 5 ~
M I_ S e t U p By.
______
INSTRUM ENTATION USED:
S la m The rm om e te r N u m b e r /t
feD r S I O . O . M a t e r # 1 ( S N E 8 0 I 4 4 7 5 )
3 CP O ig tim re pH M a ter (0 9 5 0 0 5 6 3 ) S m o n ta H l 60P W a r e (40020093) Y 3 SCT 3 3 M ater (H 8016 7 9 2 )
Q Y S 0 .0 . M ater 2 (SN 91A 026329) C om ing 2 4 0 ( t t M a te r |S 2 6 8 )
SP P ortipH 2 M alar (S 52360) Q Spartan ATC R eiracto m eter (A 366)
D v O _____________D a y I
C - O c , 5r O c , 12
Day 2
>4 ^
T 1 ,\o* 3 o . + M S
M 2 -7 M
MA
3 P
^c.5" n s
4 >eA
0 6 .0
5 - V - f 4 o U MS
C
1 ifG
2
pH
3
4
5 c
1
D .O .
2
(n g rt)
3
4
5
sC A1 l 12
N3
4
s Radicate #
Intuit:
S', i
S od
> 5 "M "M
O l, 'l
XI
)
G-M
7 SG T
7 CsV 7 r. ;
g -1 3
9, as- s . a o
7 -s'
7 S'
r '7
T--S
-7 ^
75
8 -X<X
? -r T --3 Z. \
77 6, ^
vc
i. T-
\h,
X1
Ol d
7; 'N f
^1
.a _ ;
_ y _____ C- ; i
NOTES:
Q uality A ssu ran ce:
A s Q iality A s s u w c a o ffice r for th is te s t 1 c e rtify th a t no significant
A v ia tio n s from a c c e p te d protocol o ccu rred during routine im p actio n s
( w i n s o th e rw is e n o te d below } an d th a t th e ( b te acc u ra tely refle ct th e
t e s t a s it w a s p e rf o rr m d b y CBI.
`
Correm nts
--
/? i / T
< As% - ( / /J ( /
y - r--------- r Z . i y
^
t ! M i On#
D-24
TEST I D H / '- f l 4 7 H G
US00001205
COASTAL BIOANALYSTS INC EFFLUENT/DILUTION WAT E R DATA (Fom.EFDL.Ji95)
l u r r m (ARRIVAL) EFFLUENT CHARACTERISTICS:
Sample D ate
iV ^ /s rl
1
Temp (C )
I5\l
1
Conduct. (u M H O S ) 1 S ' 7 - 1
1 1
Salinity (g/kg) I - :
l
I
pH
IS,o3
1
1
Diss. O x y g e n (m g/l) \ U M
1
1
Tot Chlorine (m g/l)
1 1 ,1
N H 3 -N (m g/l) 1 ~t . 7 Hard imu/1 C a C 0 3 ) 1 / / v
1 1
1 1
1 ... . 1 1 1 1 1
1. 1 ...
I 1 1 1 1 1 1 1 1
.... 1\ 11 11 11 11 11 11 11 11
Alkln im u/I C a C 0 3 ) 1 0 i .
I
1 _____ !---------------------!------------------------ --------------------- ----------------
C olor/A ppearance1 1 t Y
1
1
1. 1 1 1
Initials
1 <r K
1
"1
1_
1
1
1
1 C-Clear 0 - O p a q u e Y- Yellow B -B row n Bl-Black G -G re e n T -T u rb .d SI -Sol.ds (S-Sl.ghi M-Mude.Je V - v u ) n u l i .j
__ . F F F I UENT ADJliSTM ENT/POb 1-A JIJU M lw tn i
ACUTE
D ate
1 1 1 1 1 II
Temp (C )
IQo/? 1
1
i
1 1 1 II
Salinity (g/kg) l i )
1
1
1 ..- 1
1
1 II '
D 0 (m g/l)
I I 1.
1 1 II
Aeration Rate
1 1 1 II
Aeration Tim e (m in) 1 ^ 1
I 1 1 1 1 II
Adjust. D O. (m g/l) 1 ~
1
1
1
1
1 II
p H i ^ ? i i i . I 1 1 II
Tot Chlorine (m g/l) L , o
1
i
i - 1 1 1 II
Initials
1 C- H
1
1
1 .1
1
1 II
A p i itF
DILUTION W A TE R CHARACTERISTICS:
DMW:
H W M .^
D ate
l*/rt/4?l
1
!
Is
1
1
II
TemD. i C )
IS a .-s U
1 1 1 1 1 II
Conduct. (u M H O S )
1
1
1_
1
1
1
II
Salinity (g/kg) l 3 o
1
1
1
1
1 II
p H l 7 - . f a 1 1 1 1 1 1 II
Diss. O x ygen (m g/l) 1 7 - , ^ / 1 1 1
1 1 . II
H ard fmu/I C a C 0 3 ) 1
I
1
1
11
1 II
Alkln / mn/1 C a C 0 3 ) U -/I
I
1
t ______!___________ J-----------:------ !-----------------1 ----------------
Initials
KH
1
1
1 .1
1
1 II _
rt-rr~rr~u. u1 icT nV l Ti iIP . V '.1. j --<. <A_\-- --l X
,,,,SA
9 > - ' f i u t
- T E S T ID:
7 ? .no
US00001206
CHAIN-OF-CUSTODY RECORD
C L IE N T IN F O R M A T IO N
& ANALYSIS REQUEST FORM
COMPANY/OMMAND: 0 & [ / i
ujjjw ^
ZS 3)
E N V IR O N M E N T A L
PW C EN VIRO N M EN TA L LA BO RA TO RY
CODE 930 BLDG Z - 140 9742 MARYLAND AVENUE NORFOLK, VA 2 3 5 1 1 - 3 0 9 5 PH: (757)445-8851 FAX: (757)445-8852
CONTACT: \ ^ l i R L Q *-J<L
PHONE:
EXT:
J O, #: \ q \ u ^ i q D
SIGNATURE: n *\ - A 'l
PERMIT NO.:
FAX:
----
LAB U SE ONLY LINE ITEM S A M P LE NO
S A M P LE ID /LO C A TIO N
ifi-ORALi/ o c
fc c ^
o 'V -* <xi - I
SAflMi UKin SI ARI
0A1E
l-a ^
_ L iS vu
i y (-
IA S 2 t i P
-2 S TYPE
G GRAB CF - C O M P O S ITE , FLOW CT - C O M P O S IT E , TIME
A
A 2.
f\ 3 | 6^
_S2
o
<YI -1^7 (V
* /*7*-c^*
Sloe/11
P'Si i * i A / y a g
S lo p /j S P j
s u m -y ^
!>Af,l
s i* U , R A o
iiof/j qPM
A2
H o r/ r# 4
s ii
slop
M A T R IX
L - L IQ U ID
S W -S W IP E
S -S O L ID
G S -G A S
S S -S E M I SO LID
SlMPLiO er
6? ^ 5
U
<
H Of SAMPUi /(OHlAjHEAi
ANALYSIS .T e x i i'iij-
FIELD REAOINGS UHPfaAfUftf OiHEA
......UH Z l . f c
PRESCAYa HOii
vCRlNCDIi
FT
0
6? i 3l 6 S*
u
U
u~
f Y 1
.T P X ifJ ij...
Toxi.Ckiy.... ........................ IM
.....
U 'C l.?
& i l u-
(? 3 i : U
<
T
f
...... .
..JPi&y ...
.J a X '& . j...
a tm w-Q VL
i M l \ A
P PLASTIC G L -G LA SS T-TEFLO N V -V O A
C O N T A IN E R
B - BAG
A - AM BER(glass)
C CARTRIDGE
TL - T E FLO N LIN E D LID
H - HEXANE RINSED
P R E S E R V A T IV E
1 - COOL TO A C
5 -H C L , pH<2
2 - H N 03, pH<2
6 0.008% N a 2 S 2 0 3 ,1 C
3 - H 2S 04, pH<2
7 FIE LD FILTER
A NaOH, pH>12
8 NONE
iu a n a io u m o (D im ):
COMMENTS:
_____(FOR R U SH TU R N A R O U N D ST A TE RE AS O N BELOW !
"J A
R E G U L A T IO N A P P L IE D :
RCRA (
HRSD ( )
SDW A (
TSCA ( )
CWA (
PHOTO ( )
_____ L( E1V\ v e \ C t C x L. l & j,p S A M P LE D IS P O S A L; ( R E T U R N T O C L IE N T
D IS P O S A L I
'C A A V,,.5 ^ '
(
OTHER ( ) <V-A (L e i
( U & N Ov Ll (y / j ^ l<j y
I CUSTOMER IS RESPONSIBLE FOR A L L CHARGES N E CE SSA RY FOR THE PROCESSIiNr jGn Aa Nh oD AANN AA LI YYSSIISS OOFF SSaA MS P LlEEIISSI1. . ' '
'ALL R U SH SAM PLES ARE S U B JE C T T O S U R C H A R G E
SAMPLING/COLLECTION CHARGE: POSSIBLE SAMPLE H A ZA R D S : COMMENTS:
A rriv a l T e m p ,_ - s v c .
S t t e n . .... - ,
D .O . N U M B E R : CO NTRA CT LAB:
IN IT .: DATE:
C O N T R A C T N O .(S ): `OFFICiAL'U'SE'ONLY
VO (N
Q
US00001207
CHAIN-OF-CUSTODY RECORD
CLIENT IN FO R M A TIO N
l
ENVIRONMENTAL
& ANALYSIS REQUEST FORM
P W C EN VIRO N M EN TAL LA BO RA TO RY
CODE 930 BLDG Z-140
9742 M A RYLAN D AVENUE
NORFOLK, VA 2 3 5 1 1 - 3 0 9 5 PH: 1757)445-8851 FAX: 757)445-8852
C O M P A N Y / C O M M A N D : 0 . r ) U ./ f t C j> < ^ _ CODE:
CONTACT: ft ,
L
PHONE: t j f i 5 x7 , i ^ X T :
FAX:
J O | ^ | K 3R Q
SIGNATU RE: cv K - } f ^
PERMIT NO.:
LAB USE O N L Y L IN E IT E M tf |S A M P L E N O .................1 3 8 & J L * M
f-
If..................................
>...................................
t ; ....................................
IV PE
G CRAB C F . C O M P O S IT E, FLO W CT C O M P O S IT E, TIM E
S A M P L E IO /L O C A T IO N
E)3' V -L4 1 U >
uaw
C l 1 *1 OAtE { MME
SIASI
SIO?
SIAM SIO
--------------- : ---------------
rr?
SUSI SIO?
-------------! - .............
SUSi S' O?
-------------- ----- - .......
s ia ; ---------------- :---------------
s 10?
SUN
SK?
SUN
--------------- :----------------
* \io 7 "
SAHPlEO il
Xi
H A lfiU L-
?HS?.7 A llV f
`S
tf OF SAMPLES
E IE L O R E A D IN G S
/(O U I A lllf AS
A N A L Y S IS
pH 1 IE M N R A IIX 1 O M ER
1 U i . q i i......T o t i t i i y ..................................
M e s tim iV c ln j; :
1 !1
s*
m.
- Arrival T e m p . . . ,, s i ,
!! .1 1 1--
1! i !
1i 1
M A T R IX
L LIQ U ID
SW SW IP E
S -S O L ID
G S - GAS
S S - S E M I SO LID
P P LA S TIC GL - G LASS T TEFLON V - VOA
C O N T A IN E R
B BAG
A A M B E R ( g la s s )
C C A R T R I D G E
T L - T E F L O N LIN E D LID
H - H EXA N E R IN S E D
PRESERVATIVE
1 COOL TO 4 C
5 H C L , pH<2
2 -H N 0 3 , pH<2
6 - 0.003% H a 2 S 2 0 J, 4 C
3 H 2 S 0 4 , pM<2
7 F IE LD F IL T E R
4 -N aO H , pH>12
8 NONE
T U T I MO ( 0 .) O'
COMMENTS.
I F O R R U SH TURN ARO UN D STATE R EASO N UF.I.OW)
REGULATION APPLIED:
RCRA ( )
HRSD( . )
SDWA ( ) TSCA ( )
CWA ( ) PHOTO ( )
CAA ( ) OTHER ( )
S A M P IIH G /C O LLE C TIO II CHARGE: POSSIBLE SAMPLE H A ZA R D S : COMMENTS:
6
S A M P I.E D IS P O A L : ( )R E T U R N T O C L IE N T ( (D IS P O S A L OY LAD
C*" I. CUSTOMCn IS RESPONSIBLE FOR A U CHARGES NECESSARY FOR THE PROCESS/AVG AN ANALYSIS OF SAMPLE (S). ,
AI L R U SH S A M P L E S A R E S U O JE C T T O S U R C H A R G E
U
i^rasB E K r"
CONTRACT L A B f
INI f.: OATE:
C O N T R A C T N O .{S T 'OEEICICUSEOHLY '
--2--.t--SAt-M^P?.LinETS-.iR- E- CnETIVr-E.tD-iATFr.Ti-EinRTT2W:0im0fgPiicMil MI mOaNil.<THuUnRSii~. ilAirNiD 1:00 PM ON FRIDA Y WILL BE PROCESSED THE NE XT BUSINESS DA Y 17. 0 0 A M - 3:3 0 PuMe).evnuuaunt
- " FOR LAD USi: O NLY!!
SAMPLES VERIFICATION
IN ITIA L
:r-,G J E C T E D { )
R E A S O N : HOLDING T IM E ( )
C O N T A IN E R S * )
(M P R O PER LY SA M P LED ( ) O T H E R ( )
DATE.
iT T E r in D u is T T E n ir -J ;j ; u v j n ,T r ( la rLL-YL
m E u ra io is n E iro r / Y V U "
5X 770
r.v T O T O O is n in r T n - ^ a W t
lu u r in r i / X J y X / ' c . > T / V V '
r l c 'u u r
"vrrr-TTrm'- S'ix> */ r >"v . r ......... --
C U M FA N Y Z C U M M 'A N lT ' IT 0 .7 C -
TOW PAHY/CMW AW IT ' S TJOMFANYiCOMMNL) T L R -r r .n r ^ K jT T 'n r T O T K N i " M v r ; v ......
-- DA1E7TOE:
UA1L-71 IM I:
.j / .
U A T lU n U TM "^!? ,^..
iTnTTTi.V.r.
a.
d b'Y '--fifY) Vb ^ :>vluu).
f ir c .'c l b y
IbO
<N Q
US00001209
Coastal Bioanalysts Inc.
O D U /O ceana Acute Effluent Toxicity Tests
Mysidopsis bahia & Cyprinodon variegatus
Sample I.D. A1 Contract No. N00187-93-D-9748
* Delivery Order No. 0235 J.O. No. 1912290, Item No. 0002BJ, 0002BH, 0003AD
PWCNo. 1353 9/28/97 1700 Sample No. JRA97-10762
Submitted to:
Ms. Merrill Anderson-Ashcraft Navy Public Works Center Code 930, Building Z-140 Norfolk, VA 23511
By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062
(804)642-0168
D-29
US00001210
O D U O C E A N A Sample I.D. Al 9/2S/97 Sample 97-10762 T e a ID: JRA 97111
Coastal Bioanalysts Inc.
I. TEST RESULTS
% Effluent Concentration
0
6.3
12.5
25.0
50.0
100
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acute Test Results
Mean (%) Organism Survival
M . bahia
C. variegatus
24-h 48-h a 95 95
24=h 100
48-h 100
100 95
100 100
95 95
100 100
95 95 100 100 95 90
100 100 100 100 100 100
>100% N/A
' N/A <1.0
>100% N/A N/A <1.0
Acute Test Information:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
M . bah ia Test 48-h Static Survival 9/30/97 1145 10/2/97 1145 CBI stock cultures 9/30/97 20 g/kg 19.5-20.7C
No N/A
C variegatus Test 48-h Static Survival 9/30/97 1200 10/2/97 1200 Aquatic Bio Systems, Inc. 9/26/97 20 g/kg 19.5-20.7C No N/A
1Aeration necessary when effluent oxygen demand results in dissolved oxygen concentration < 4.0 mg/1.
1
D-30
US00001211
ODU'OCEANA Sample I.D. A! 9/28/97 Sample 97-10762 T e a ID: JRA97111
Coastal Bioanalysts Inc.
Sample Collection Date/Time
9/28/97 1700
Sample Use Dates/Times
9/30/97 1145/12003
Aeration2 R a te /Iim g
n/a
Pretreatment/ Adjustment
HWM Salts
2Aeration often necessary to reduce sample oxygen concentration to 100% saturation after wanning. 3.W. bahia/C. variegatus test time.
Table 2. Effluent and Dilution Water 4 Chemical & Physical Characteristics (Mean Value (Standard Deviation))
Parameter
Temperature (C)
Salinity (g/kg)
Conductivity (uMHOS/cm)
PH
Dissolved Oxygen (mg/1)
Total Hardness (mg/1 as C aC 03)
Alkalinity (mg/1 as C aC 03)
Total Chlorine (mg/1)
n h 3-n (mg/1)
Initial Effluent
3.4 (-)
N/A
1479 (-)
7.89 (-)
11.5 (-)
118 (-)
248 (-)
<D.L. (-)
6.9 (-)
Adjusted Effluent 20.7 (-) 21 (-) N/A
7.75 < -) 8.0 (-) N/A N/A
N.D.
N.D.
Dilution W ater 20.8 (-) 20 (-) N/A
7.90 (-) 7.9 (-) N/A
N/A
N.D.
N.D.
4Effluent parameters measured upon receipt of sample in lab and after adjustment on day of use. Dilution water (Wimex hw-Marinemix in ASTM Type I deionized water) values measured after preparation of diluent on day of use.
D-31
US00001212
ODtVOCEANA Sample I D. Al 9/2S/97 Sample 97-10762 T e a ID: JRA 97111
H. WATER QUALITY
Coastal Bioanalysts Inc.
Parameter Temperature
(C) DO. (mg/1) pH
Salinity (g/kg)
Parameter Temperature
(C) D.O. (mg/1) pH
Salinity (g/kg)
Control
20.6 (0.3)
7.8 (0.2)
7.74 (0.17)
20 (0)
Table 3 Water Quality Summary t
M . bakia Test
Mean Value (Standard Deviation)
6 3%
12.5%
25%
50%
20.6 (0.2)
20.5 (0.2)
20.5 (0.2)
20.6 (0.2)
7.7 (0.2)
7.73 (0.18)
20 (0)
7.7 (0.3)
7.77 (0.18)
20 (0)
7.6 (0.4)
7.87 (0.08)
20 (0)
7.6 (0.3)
7.90 (0.14)
21 (0)
100%
20.6 (0.1)
7.5 (0.5)
7.96 (0.19)
21 (0)
Control
20.4 (0.5)
7.8 (0.2)
7.79 (0.12)
20 (0)
C . variegatus Test
Mean Value (Standard Deviation)
6 3%
20.4 (0.6)
7.8 (0.2)
7.79 (0.13)
20 (0)
12.5%
20.4 (0.5)
7.7 (0.3)
7.80 (0.13)
20 (0)
25%
20.4 (0.3)
7.7 (0.2)
7.86 (0.09)
20 (0)
50%
20.5 (0.4)
7.7 (0.2)
7.94 (0.12)
21 (0)
-
100%
20.4 (0.5)
7.6 (0.4)
7.95 (0.17)
21 (0)
D-32
US00001213
ODU.'OCEANA SampleI.D. A1 9CT97 Sample97-10762 Test ID: JRA97111
ffl. QUALITY ASSURANCE
M. bahia - Acute
Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.). Control Chart LC50 (95% C.L.):
C variegatus - Acute
Reference Test Dates: Reference Toxicant: . Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
Coastal Bioanalysts Inc.
CBI Stock Cultures 9/16/97-9/18/97 KC1 (Sigma "Ultra" Lot#76H10391) 343, 490, 700, 1000 mg/1 100% 651.8 mg/1 (605.9-701.2) 611.0 mg/1 (483.0-739.0)
Aquatic Bio Systems (same batch as test) 9/30/97-10/2/97 KC1 (Sigma "U ltra" Lot#76H 10391) 432, 720, 1200, 2000 mg/1 100% 1200.0 mg/1 (1070.5-1345.2) 1067.9 mg/1 (903.6-1232.2)
gj
D-33
US00001214
CUMULATIVE DATA SUMMARY NPDES Permit #: N/A Oceana - ODU A-I
Date of . Invertebrate: -
2/12/97 >100 2/28/97 Invalid 3/12/97 >100 3/20/97 >100 3/26/97 >100 9/30/97 >100
; Vertebrate _r '
>100 >100 >100 >100 >100 >100
Invertebrate
- Vertebrate NOEC.% Y
*
Supplement
D-34
US00001215
C O A I.T A J.H H >. YNAJ Y STS. INC N PD liS STA TIC A C U TE/SA LTW A TER
(F o rm A M !J4 * )
\ ErMuent
Oay
9 Live
Oay 2 H iv e
Control
6.2 5 1 2 .5 25 50 100
Cl '1 C-2 1 O 1! 1 O 1-2 ( J 21 1 2-2 1 O 3-1 ! O
'! 10 C*
r
___L G _______
- 5 -------------
16
I
3 -2 ______ i - _
4-1 1 O _ _ I A ______
4-2 1 O
10
5-1 5 -2 O bserver Tune:
1 O - 4 ________
c iiJ_______ J l ________ - u ______ ^ ' i L \ ____ I f Y S T
Final \
Survival
R*
V?
(00 Qa l r
"
H atch O atafT irre:
Sow ee:
7 / 3o / ? 7
CBI
A cclim ation Tam p, (range):
/<*_ s
~7
J'
C h i i r t a f s u e : 3 0 0 ni B
I T i t onv .
5 0 0 tri
1 0 0 0 ni Q
0 t h * : ______________ ri
T n t voli*TM : 2 0 0 r r t 3
? 5 0 tri Q
5 0 0 nd Q
O t h a c ______________ tri
In itia l n o . ctrpnism p o r r e p li c a te :
/ O ________ N o . TM p l i c a u * : _ ^ ____________
Photopenod 16 1 :8 0 IV I Q ttw :
R a ru ty ru e tia n terrydata no. H " t, n i
S E T U P : 0 a te _ i h o h i
H i m W a w A d * d / O 0 ___________
T im t A nim ais A d d _
h y5
S*c U p Bv f 6
INSTRUM ENTATION USED:
S iam T h en ro rro ter N urr ec A } YSl 0 .0 . M ater $ 1 (SN E 801447S)
F j c P O igisense pH M o to r (0 9 5 0 0 5 6 3 ) S in o n R 160P W (40020093) YSl SCT 3 3 M iw (H 8016 7 9 2 )
0 l r 3 0 .0 . M ater $ 2 ( 9 1 A 026329J
H C om niq 2 4 0 pH M a ter (S N 5268)
S F P o rtx H 2 M .w (8 S 2 3 S )) p H < > u n ATC R fn c to rm t r (A366)
Ottwr
C
T
E 2
M 3
4
5
C
pH
0.0.
( ir a 'I )
2
3 4
5
C
1
2
3
5
SC A1 L
2
N3
4
5
1R e p lic a t e
.
In itia ls:
Oay 0
PA S n i-.S 7o 7 >> 7 P ep
p<> 9
n 7 $7 7 .< P lb
n. 77 5 -A ? 7 -?
7-7 7? 7 *7
-fc Pd Per PC PO P, Ji / To
NOTES:
Oay I
Oay 2
06 I
P ( . V*
7fi 7 P<9b PoP
-9 6 , y a ^ . Pa ? PdP
.76-9 7 .7 7
j? . . r
7 7? 7. #5
^ S 'J 7 -.ro
% 0j.
rt > y > " .P/ T " . > 7 -.? 7 -.7
7 . ^6
? .v r
(f.C I
7 -(r
7r ?v
7- ^
7_ 7 -v -
? -.A _ 7 -7
fJL _
P 9 _____ Pc P a
Pc 7 r -
___
pi
PI
p
f - 3 _ b : _____
Q uality A ssurance: A s Q uality A u u v c i o H < a r to r this t s t I certify th a t no sejreficaiit d o ta tio n s fro m a c c e p te d p ro to co l o c cu rre d <J*ing ro u tin e in sp e c tio n s {tffess otherw ise r te d W o w ) an d th a t the a t a accurately roH act the te s t as it w as perform ed by C 8l.
O A T L . Ci. l * * 1
T E S T i n - v T K A C{ 1 1 ' 1
D-35
US00001216
COASTAI. BIOANAI.YSTS. INC. NPDKS STATIC AC1TE/SALTW ATER
(Form ACV4X)
o o
% E ffluent
1.0.
C ontrol
6.2 5 12 5 25 50 100
C-l C-2 U 1-2 2-1 22 3-1 3-2 4-1 4-2 51 5-2 O bserver T im :
Day 1 IfY*
0y 2 1 Uve
F m ril Survive!
i<s iO
la 1
1b
Io
1o
/o
1O 1O 1b ib
la
t 'O
la
| O
io
U
lO O
to
0
1b
lO O
(0
ib
f<5>
1i
.. l
Crff-
iC
K
t3H5
Q o o __
vvem *
C v p rin o d o n v a rie q a tu s
Source:
~ 0 A^iS
A cclim ation w a t e r HWM ASW 3
O th er
l t- s
.n
Satim nr. P
*>n
F e e n o p n o r to te s t:
A riern -- J X
Id O th e r.
Feedng dunng test: Not fed 0
A rtem a
Id O t h e r
C ham ber sire: 3 0 0 id
5 0 0 tri
1 0 0 0 rri v
O ther.
nd
T es t volum e; 2 0 0 iri Q
2 5 0 rH
5 0 0 rrf L;r
O th er
rri
N o. n r i i c a t e s : r-J
,
Photopw rvxt 1 6 1 :8 0
O rtw r
9FT U P: O ote
1 3>0 / ? ?
R an d x n n tK jn te n d a le no. " 5 (
1 1 3 ST i n . W a te r A d f e d
T i n A m ir a f s A d d e d ______ ! * 2
________ S ) t U p 9 v
P^
INSTRUM ENTATION USED:
S t o m T h e r m o m e te r N u m b e r ft I
T S 0 .0 . M eter # 1 (S N E B 0 1 W 5 ) O i se n se pH M e ter (0 0 5 0 0 5 6 3 )
S artonus R 1 6 0 P B alance (4 0 0 2 0 0 9 3 ) Y S SCT 3 3 M otor (H 8 0 1 679 2 )
0 . YSI 0 . 0 . N W # 2 (S N 9 1 A 0 2 6 3 2 9 ) I I Com ing 2 4 0 pH M e te r (S 2 6 2 6 8 )
S ' PortapH 2 M o to r I8 S 2 3 6 0 ) J ^ S | o n H i ATC R afraclo m eto r (A 368)
O th o r ____________________ ___________________________ __ _____________________________________________
C T E
2 M
3 p
5 C
2
PH 3
4
5
C 1
0 .0 . (m gfl)
2 3 4
5
sC
A1 1
2
N3
4
5
R eplicate t
In itia ls :
0ay0 - 2 0 .T
Q*-'S ^L /
P e -c
Po 7
.?*> k
7 .5 ? 7. J;
7 / / ) 7 i> 6 7 P
7 -7 5 7 .^ 77
77 77 77 /O m Ac
C _P0
P 'S 7o
.7 -? i
/
PO
Oay 1
Oay 2
P i 6' i r
pc?
ff-f /1 ?
7 0 6 ... P0 7
p o .6
P d -%
7 06,
7 yY 7 5? 7^ S . C 1?
?- t i V 7 fa 5 7 .7 ^
S' . 0 6
> ? 'f A , > U, { -io PO P P '2
Pi
P p ip
H
> i- x -
-4
_
?3 _ P f,
________ -X o iA J /
nt
7> v -P
N O TES;.
Q uality A s s u m e :
A s Q u d ity A s s u m e s e H ie a r for th*s t e s t I c e rtify th a t no s ig ru ficm t (tew atio n s fro m a c c e p te d protocol o ccu rred danng routine in sp ectio n s (m lM s o th e rw is e n o t d b e k ~ ) an d th s t the A sti accu rately reflect th* te s t a s it w e s p e rfo n m d ty CSL
C om m ents
S*- ~
~> I C k ^ n . - - - lY
, -u. W .
D-36
T E S T I Q - ~~\v<. A ^ " 7 1 I
I
(
US00001217
COASTAL B I O A N A L Y S T S INC.
EFFLUENT/DLLUTION WATER U A .A
INITLAL (ARRIVAL) EFFLUENT CHARACTERISTICS:
Sample Date 1
Temp (C) Conduct. (uMHOS) | I */ ? ^
Sr aTlin_i7ty_ 7(g7/Zk7g )\ I C /'
pH IT . J?
Diss. Oxygen (mg/1) | f / . 5 " T ot. C hlorine (mg/1) | c tD L _
N H 3-N (mg/1) | O H ard (mg/1 C aC Q 3 ) | I / ^ AJkln (mg/1 C aC Q 3 ) 1o? V ^ Color/A ppearance1 C Y
Vh
1 r J - J " 1"o L TM Y . L l L r B -B ,,w ,, Bi-Black J o ,c e ,, T -T ., ,d M - * * * ,S-Si,e h.
EFFLUENT ADJUSTMENT/POST-APJUSTMENT MEASUREMENTS:
v^do ACUTE
EFFLUENT ID: (
______________ T E S T m -
_ D-37
A `O 1 l_L
US00001218
CHAIN-OF-CUSTODY RECORD
& ANALYSIS REQUEST FORM
PW C EN VIRO N M EN TAL LA B O R A TO R Y CODE 930 B LD G Z-140 9742 M ARYLAND A VEN U E
CLIENT INFORM ATION
p.nMPANY/COMMAND: 0 0 \ A lf)C j> a > S 0D^L
c o n ta c Y aV u ^
EXT:
FAX:
J.O. #: SIGNATURE: p
FNVm nNM FNTAl
PH: 17571 4 4 5 -8 6 5 1 F A X : ( 7 57)4 4 5 -8 8 5 2
''fcrvIVll I INU.. ..]!
LAB USE ONLY
SAMPLE IO/LOCATION
1AHPU OH UH 0A
Al ITPE SAHPliO
I a of SAMPUS
IHE M HAtiU rHESEKvAllVE I /COHlAIHiAS
ANALYSIS
pH I TEHPERAtUAE | 0*HEB
,( ft& A l..|, o r M
1 ( ->
i l,._
C^s
...... TM _ I L L _ ______
L_L2 2 Ils
T TJl? p d f* try
1 1 QC*
\A \fZ U \-
ft \oC-- * 0
I / a i - m v ( -/o T U U
SURI StOP : ij sif i- F V
-> sum . W a g
i*i-7 -% < t
w -f% v
SIAK tA /l/A ti siop/i rfM SIASI A7 / 2-Z S lop p M . -
b *5
u
0 ur
6u
6 & u~
r
<U - -
KI -------- U^--- (/ :d \ [ o r
I 4-7 l
i.tq I
....T e n a c i t y ........................ I 7 .^ 1
J f c X i t U f y -- ........ IM
l \ A
....r o . & . ' . & . t y . .................... Im
ti.O
....T P i o i t y ...................... I
i M sl\AJ t e i z t f .......................
PREilPVAflOH VE8IHCQ8T || Kjj bv^y-- I i --
u
TYPE
G GRAB CF - COMPOSITE, FLOW CT-COMPOSITE, TIME
B - BAG C CARTRIDGE T L - TEFLON LINEO LID
1 - C O O L T O i4- HC C L . p R ^
2 - H H 0 3 , pH <6 2. 0 . 0 0 8 % N a 2 S 2 0 3 , 4 C
3 H 2 S 0 4 , pH<2
7 - F IE L D F IL T E R
4 - N iO H , pH>12
0 -N O N E
tu.HAiOUHO (Oiril:_______(FOR RUSH TURNAROUND STATE REASON BELOW)
COMMENTS:
------3 a d ^ :_ /& A !? A
REGULATION APPLIED:
RCRA ( ) SDWA ( )
HRSD( ) TSCA ( )
CWA ( ) PHOTO ( ) CAA ( ) OTHER ( )
S A M P LE O IS P O S A L : ( (R E T U R N TO C L IE N T ( (O IS P O S A L B Y LA B
31
-/a -L A
*-R -C _ 'W it "i / j s / ;
, CUSTOMER IS RESPONSIBLE FOR ALL CHARGES N E C ESSARY FOR THE PROCESSSIINNGO AND ANAL YSIS OF SAMPLEISI. '
I SAMPLING/COLLECTION CHARGE:
POSSIBLE SAMPLE HAZARDS^ I COMMENTS:
Arrival Temp,
16.0.KUMUL'r
Ic O N T R A C T L A B i^
C O N T R A C T N O .( S ) : ______ o F F ig A c u s ro n rr
' ..
iT a io T T "
REASON;
HOLDING TIM E ! ,
SA M P LES V ER IFICA TIO N
C O N ,A ,HERS, I
IMPROPERLY SAMPLED, I
OTHER! _)
INITIAL: D A TE:
IRKLIN(JUISMkL) U ^ :'" T || RELINQUISHED U V ^ - n - A
IL
|REC` DY: / - y \
COMPANY/COMMAND: (.T )
" 'DAT/TIME: L{ /J H M i
00 CO
I
Q
US00001219
US00001220
CHAIN-0F-CUSTODY RECORD
& ANALYSIS REQUEST FORM
PWC ENVIRONMENTAL LABORATORY
C O D E 9 3 0 B LD G Z-140
9742 MARYLAND AVENUE
ENVIRONMENTAL
N ORFOLK, VA 2 3 5 1 1 -3 0 9 5 P H : 17 5 7 1 4 4 5 - 8 8 5 1 F A X : 1 7 5 7 1 4 4 5 - 8 8 5 2
LAB USE ONLY
L IN E ITEM s a m p l e NO
CLIENT INFORMATION
COMPANY/COMMAND: O A u / ) ^ , ,, ,, CODE:
CONTACT: ft \ -fcJl y-(^~ L
PHONE: v? WsT/^7 -it-pXT:
j.o, # |c^ | LL i! 7
FAX:
SIGNATURE: *" Q i'\ PERMIT NO :
i vs
TYPE I: r . . g r a b
C F C O M P O S IT E, FL O W C T C O M P O S IT E, TIM E
M A T R IX
L LIQ U ID S S O LID
S W . SW IP E
G S GAS
S S - S E M I S O LIC
P P LA S TIC G L G L A S S T-TEFLO N
C O N T A IN E R
8 DAG C . c a r t r id g e
A A M B E R (g la ss)
TL TEFLO N LIN ED LID
P R E S E R V A T IV E
1 . COOL TO 4 C 2 H N 03, pH<2
5 H C L, pH<2
6 0.003V, H a 2 S 2 0 3 , 4 '
3 H 2 S 0 4 , pH<2 4 NaOH, pH>12
7 . F IE L D F IL T E R
0. NONE
K r.K i.C C .U H D
COMMENTS:
( P O R R U S H TURN ARO UN D S TA TE REASO N UELOIV)
REGULATION APPLIED:
RCRA ( )
HRSD ( )
SDWA ( )
TSCA ( )
CWA ( ) PHOTO ( )
C M ( ) OTHER ( )
S A M P LIN G /C O LLEC T IO U C H A R G E: P O S S IB LE S A M P LE N A7.A RO S: COM M ENTS:
S.R -.--FLf: D I S P O S A L : ( ( R E T U R N T O C L I E N T ( ( D I S P O S A L U Y L A B
. c, --
fSS" Krn THEPttC5S,,'`C
J _ S A I A r U S RECEIVED A T T fR 1.00 PAI M O N . ---------
! 1 ' F O n LAD U S E ONLY!!
[R__EJ_E~C~TED! ' I REASON:
H O L D IN G T IM E { )
SAM PLES CC OO NN TT AA IINN EERR SS ! )
/ v_o-T d AU
[K O O B B E R ? C O N T R A C T L A B : --------7-------------------
C O N T R A C T N0.(ST""
'om cm coTTuncr
THENEXTBUSINESSVDA^ Y,7) :00AM^olsoTw.
- ------- IIW
nac i i i,a. uwAaignjx
V ER IFICA TIO N
O TH ER! )
IN IV .: D A TE:
/j' O -K '
A Z a-X
U A lb /lIM fc .? /^ --? / / , u a il n im
--------------
n m r W ^ j : ^ vl ^
------------- CORTOTV7CUKII/AIII.I-
____ --
UA.LtTIJL
bv-.=9m I k t i
fe e d by : /T J K r v
i ^ T / v iu
Os
fO I
Q
D-40
US00001221
Coastal Bioanalysts Inc.
O D U /O ceana Acute Effluent Toxicity Tests
M ysidopsis bahia & Cyprinodon variegatus
Sample I.D. A2 Contract No. N00187-93-D-9748 Delivery Order No. 0235 J O. No. 1912290, Item No. 0002BJ, 0002BH, 0003AD
PWC No. 1354 9/28/97 1700 Sample No. JRA97-10763
Submitted to: Ms. Merrill Anderson-Ashcraft
Navy Public Works Center Code 930, Building Z-140
Norfolk, VA 2351 1
*
By Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062
(804) 642-0168
D-41
US00001222
O D U O C E A N A Sample I.D. A2 9/23/97 Sample 97-10763 Test ID: JRA97112
Coastal B ioanalysts Inc.
I. TEST RESULTS
% Effluent Concentration
0 6.3 12.5 25.0 50.0 100
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acute Test Results
Mean (%) Organism Survival
M . bahia
24-h 48-h 100 100 100 100 100 100 100 95 100 95 100 90
C. variegatus
4M
100 100 100 100 100 100 100 100 100 100 100 100
>100% N/A N/A <1.0
>100% N/A N/A <1.0
Acute Test Information:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
M. bahia Test 48-h Static Survival 9/30/97 1245 10/2/97 1300 CBI stock cultures mom 20g/kg 19.5-20.7C
No N/A
C variegatus Test 48-h Static Survival 9/30/97 1255 10/2/97 1315 Aquatic Bio Systems, Inc.
9/26/97 20 g/kg 19.5-20.7C
No ^ N/A
'Aeration necessary when effluent oxygen demand results in dissolved oxygen concentration < 4.0 mg/1.
D-42
US00001223
CHAIN-OF-CUSTODY RECORD
CLIENT INFORMATION
ENVIRONMENTAL
& ANALYSIS R EQ UEST FORM PWC ENVIRONMENTAL LABORATORY CODE 9 3 0 BLDG Z - 140 3742 MARYLAND AVENUE NORFOLK, VA 2 3 5 1 1 - 3 0 9 5 PH: (7571 4 4 5 -8 8 5 1 FAX: (7571445-8 852
COMPANY7COMMANO: O A U ' f ) 0 , a ^ _ CODE;
CONTACT: \ , V I
L
PHONE: A -j/C /V c : _0yXT:
FAX:
JO .#: |<^ \
SIGNATURE: o, ,.v ^ t fo PERMIT NO :
LAB USE ONLY
IN E ITEM .S A M P L E NO
W
| i vpe'
i; C G " A8 C F C O M P O S IT E, PLO W C T C O M P O S IT E. TIM E
S A M P LE ID /LO C A TIO N j&> 3 < y . / c m
MAPI $10? St A?l WO IA R I SI0P SUM $10? sua; $10.
3 k t\ M
$u?i S10P SlA'f f
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L - LIQ U ID
SW . S W IP E
S S O LID
G S-G A S
S S - S E M I S O LID
Gr 3 5 ~
p . P LA S T IC GL G LASS T- TEFLO N V -V O A
u
n Of sKPUi
/(OHUIHEflS
A N A LYSIS
..Tx.i.cUivj.
FIELD R EA D IN G S pH 1 U H PtflA lU f O I K E
PREH*vi:' VtRIfliO 1`
Arrivallemp,__ .T - c
con tain er
B D A G
A - A M B ER (g la ss)
C -C A R T R ID G E
TL TEFLO N LIN ED LID
H H EX A N E R IN S ED
P R E S E R V A T IV E
1 COOL TO C 2 -H N 0 3 , pH<2
5 - H C L , pH<2
$ 0. 003% N a 2 S 2 0 3 ,
3 H 2 S 0 4 , pH<2
7 F IE L D F IL T E R
4 - NaOH, pH>12
3 - NONE
_______
K irK A .C C U H D
COMMENTS
(FOR RUSH TURNAROUND STATE REASON ULO\V)
F REGULATION APPLIED:
RCRA (
HRSD ( )
SDWA (
TSCA ( )
CWA (
PHOTO ( )
CM (
OTHER ( )
"1
S A M P LE D IS P O S A L : ( )H E T U R N T O C L IE N T ( )D lS P O S A L B Y LA B
ex--- ( L jt t f c w /. CUSTOMER IS RESPONSIBLE FOR ALL CHARGES NECESSARY FOR THE PROCESSIN,'G ANO ANALYSIS OF A M P E E SI. .
Al l. R U SH S A M P L E S A R E S U B J E C T T O S U R C H A R G E .
Q
SA M PLIK'G /C O LLEC TIO N C H A R G E: PO S SIB LE SA M P LE H A Z A R D S : COM M ENTS:
.in m u tv o t* fffC T D H W R ?
C O N T R A C T L A B :*
IN I.: O A T E :'
C O N T R A C T N O .(S ): VFFrciicv5Fvm .y--
2 . SAMPLES P.ECEIVEE) AFTER 1 :0 0 PM M O N . ` fo n LAB USE ONLVI!
THURS. ANO 1:0 0 PM ON FP.IOAY WILL BE PROCESSED THE NE XT BUSINESS D A Y (7 :0 0 A M J.W Tf wtc*SO.T1TMnjxuiw i t w i i t .**Ju r. j llm.h i w w q m o . "ut u m 'jL tw S A M P LE S V ER IFICA TIO N
REJECTED) ) i REASON: HOLDING TIME)
CONTAINER5( )
IMPROPERLY SAMPLED( ) OTHER(
3 :3 0 PM).
IN IT IA L: OATE:
2 .i'\i(? , t h f i A
|| iTr.nnT)oisnfcU~iiV
w m m tJSiJ l'iT T .I I .O U I S H c U 'F r ' fa'in y T iS T -iiT c rc T r
- iL iA c x jt
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(^ rrg Z ^ I
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D-44
US00001225
Coastal Bioanalysts Inc.
ODU/Oceana A cute Effluent Toxicity Tests
M ysidopsis bahia & Cyprinodon variegatus
Sample I.D. B3 Contract No. N00187-93-D-9748
Delivery Order No. 0235 J.O. No. 191^290, Item No. 0002BJ, 0002BH, 0003AD
PWCNo. 1358 9/28/97 1700 Sample No. JRA97-10767
Submitted to: Ms. Merrill Anderson-Ashcraft
Navy Public Works Center Code 930, Building Z-140
Norfolk, VA 23511
By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062
(804) 642-0168
D-45
US00001226
ODU'OCEANA Sample .D. B3 9/28/97 Sample 97-10767 Test ID: JRA 97116
Coastal Bioanalysts Inc.
I. TEST RESULTS
% Effluent Concentration
0 6.3 12.5 25.0 50.0 100
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acute Test Results
Mean (%) Organism Survival
M . bahia
C. variegatus
24-h
R
100
4SJl 100
24-h 48-h 100 100
100 100 100 100 100 95 100 85 85 10
.'
100 100 100 100 100 100 100 100 100 100
65.0% 53.7-79.9
Probit 1.54
>100% N/A N/A <1.0
Acute Test Information:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
M. bahia Test * 48-h Static Survival 9/30/97 1325 10/2/97 1400 Chesapeake Cultures, Inc. 9/30/97 20 g/kg 19.5-20.7C No N/A
C variegatus Test
48-h Static Survival 9/30/97 1315 10/2/97 1320 Aquatic Bio Systems, Inc.
9126191
20 g/kg 19.5-20.7C No " N/A
'
Aeration necessary when effluent oxygen demand results in dissolved oxygen concentration < 4 .0 mg/1.
D-46
US00001227
ODU/OCEANA Sample I.D. B3 9/2S/97 Sample 97-10767 Test ID: JRA97116
Coastal Bioanalysts Inc.
Sample Collection Date/Time
9/28/97 1700
Sample Use Dales/Times
9/30/97 1325/13153
Aeration2 Rate/Time
n/a
Pretreatment/ Adjustment
HWM Salts
2Aeration often necessaiy to reduce sample oxygen concentration to 100% saturation after -wanning. IV/ bahia/C. variegatvs test time.
it .
Table 2. Effluent and Dilution Water 4 Chemical & Physical
Characteristics (Mean Value (Standard Deviation))
Parameter
Temperature (C)
Salinity (g/kg)
Conductivity (uMHOS/cm)
pH
Dissolved Oxygen (mg/1)
Total Hardness (mg/1 as CaC03)
Alkalinity (mg/1 as CaC03)
Total Chlorine (mg/1)
n h 3-n (mg/1)
Initial Effluent
3.1 (-)
1 (-)
1639 (-)
7.16 (-)
11.1 (-)
147 (-)
281 (-)
<D.L. (-)
25 (-)
Adjusted Effluent 20.9 (-) 20 (-) N/A
7.24 (-) 8.0 (-) N/A
' N/A
N.D.
N.D.
Dilution W ater 20.7 (-) 20 (" ) N/A
7.84 (-). 7.9 (-) N/A
N/A
N.D.
N.D.
4Effluent parameters measured upon receipt of sample in lab and after adjustment on day of use. Dilution water (Wimex hw-Marinemix in ASTM Type I deionized water) values measured after preparation of diluent on day of use.
D-47 j
US00001228
O D U 'O C E A N A S am p le I.D . B 3 9/2S/97 Sam p le 9 7 -1 0 7 6 7 T e st ID : J .A 9 7 1 16
O. WATER QUALITY
Coastal Bioanalysts Inc.
Parameter Temperature
(C) DO. (mg/1) PH
Salinity (g/kg)
Parameter Temperature
(C) D.O. (mg/1) pH
Salinity (gAg)
Control
20.7 (0.2)
7.8 (0.1)
7.78 (007)
20 (0)
Table 3 Water Quality Summary
M . bahia Test
Mean Value (Standard Deviation)
6.3%
12.2%
25%
50%
^0.7 (0.2)
20.8 (0.2)
20.7 (0.1)
20.7 (0.1)
7.8 (0.1)
7.60 (0.17)
7.7 (0.2)
7.62 (0.11)
7.7 (0.3)
7.67 (0.15)
7.6 (0.3)
7.71 (0.33)
20 20 20 20 (0) (0) (0) (0)
100%
20.8 (0.2)
7.5 (0.4)
7.69 (0.41)
20 (0)
Control
20.7 (0.1)
7.8 (0.1)
7.71 (0.16)
20 (0)
C. variegatus Test
Mean Value (Standard Deviation)
L2%
20.8 (0.1)
7.8 (0.1)
12.2%
20.9 (0.1)
7.8 (0.1)
25%
20.8 (0.1)
* 7.7 (0.2)
20%
20.8 (0.1)
7.7 (0.3)
7.60 (0.15)
7.58 (0.11)
7.66 (014)
7.69 (0.34)
20 20 20 20 (0) (0) (0) (0)
-
100%
20.7 (0.1)
7.7 (0.3)
7.64 (0.37)
20 (0)
D-48
US00001229
O D I'/'OCEANA Sample I.D. B3 9/28/97 Sample 97-10767 Test ID: JR A 97116
m . QUALITY ASSURANCE
M. b a h ia - Acute
Reference Test Dates:
Reference Toxicant:
Test Concentrations:
.
Control Survival:
LC50 Reference test (95% C.L.):
Control Chart LC50 (95% C.L.):
C variegatu s - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
Coastal Bioanalysts Inc.
Chesapeake Cultures (same batch as test) 9/30/97-10/2/97 KC1 (Sigma "Ultra" Lot#76H10391) 343, 490, 700, 1000 mg/1
100%
596.7 mg/1 (548.4-648.6) 612.8 mg/1 (486.5-739.1)
Aquatic Bio Systems (same batch as test) 9/30/97-10/2/97 KC1 (Sigma "Ultra" Lot#76H10391) 432, 720, 1200, 2000 mg/1
100%
1200.0 mg/1 (1070.5-1345.2) 1067.9 mg/1 (903.6-1232.2)
D-49
US00001230
COASTAL BIOANALYSTS INC.
EFFLLTENT/DILUTION WATER DATA (Form Hi 01 oiv5>
INITIAL (ARRIVAL) EFFLUENT CHARACTERISTICS:
Sample Date l ^ / D ^ I
1i
1
1
.Temp (C ) 1 4 . 1
1
1
!
1 11
Conduct. (uMHOS) ( ( * 3 7 1 1 1 1 i
1
Salinity (g/kg) 1 /
1
1
1
1
1
1
PH ? " ' L ) 1 1 1
11
Diss. Oxygen (mg/!) K M
1
1
1
i
!
1
Tot. Chlorine (mg/1) L o L I 1 1 1 1 1
NH3-N (mg/1)
i i 1 111
Hard (mg/1 CaC03) i y k . . i i 1 1 1 1
Alkln (mg/1 CaC03) | 0} Y 1 1 1 1 1 1 1
Color/Appearance1 Y
1
1
111
Initials
C rH
i
ei
1
1
11
1 C-CIcar O-Opaque Y-Ycllow B-Brown BI-BIack G-Grecn T-Turbid SI -Solids (S-Slighi M-Modcrate V-Very Much)
EFFLUENT ADJUSTMENT/POST-ADJTJSTMENT MEASUREMENTS:
Date 11(3#^ I I I I
Temp. (C ) ISI.1 I
!
!
ISalinity (g/kg) lo?Q I I I
D O. (mg/1) I S . O I I I I
Aeration Rate
I-
I
II
I
Aeration Time (min) I -- I I I
IAdjust. D O. (mg/1) --
I
I
I
PH H -.^y I I I
I ITot Chlorine (mg/1) a ,/r?
II
Initials
I C-tf I
I
I
!
I I I I
DILUTION WATER CHARACTERISTICS:
Date
I
Temp. (C ) I , 7 . I
I
Conduct. (uMHOS) | v ,a
|
I
Salinity (g/kg) l I
I
PH
IM H I
I
Diss. Oxygen (mg/1) I M
I
I
Hard (mg/1 CaC03) i top) I
I
Alkln (mg/1 CaC03) 1n .f i \
\
DMW:
I
I
I ! I I I |
H W M -y I I
I I |
Initials
\w
II !
I I I I I I I I I I
*i
I
I I 1 I
I I I I I I I I I I
I I I I
I
I
I-
1 I
ACUTE
II II II II II II II II II II
ACUTE
II II
II
II -
II
II
II
II II
EFFLUENT ID:
__________________
0tV -
cQ - ' l o A T
TEST ID: D-50
'T it A 9 7 III?
US00001231
CHAIN-0 F-CUSTODY RECORD
E N V IR O N M E N T A L
& ANALYSIS REQUEST FORM
PWC ENVIRONMENTAL LABORATORY
C O D E 9 3 0 BLDG Z-140
9742 MARYLAND AVENUE NORFOLK, VA 2 3 5 1 1 - 3 0 9 5 PH: (75 7 )4 4 5 -8 8 5 1 FAX: (757)445-8852
LAB USE ONLY
LINE ITEM P [SAM PLE NO
lU lH A lO U M O (Oi t i ):
COMMENTS
(FO R R U SH TURNARO UND S TA TE REASON BELOW)
jL c
ia .u i ^ Q
h ik e * \
REGULATION APPLIED:
RCRA (
HRSD ( )
SDWA (
TSCA ( )
____Jl' khy\ Vv . Jl&n -- r( U/ W2 & lp. e SF l . I____________________ )
SAMPLE DISPOSAL ( (RETURN TO CLIENT ( (DISPOSAL V LA8
CWA ( CAA (
PHOTO ( ) OTHER ( )
aji O a a w -c_
(U J ig
a ,/, s/ , 7
I CUS TOMEA IS RESPONSIBLE FOR ALL CHARGES NECESSARY FOR THE PROCESSING ANO ANALYSIS OF SAMPLEISI. * ' ALL RU SH SA M P LES A R E S U B JE C T TO SU R C H A R G E.
SA M P U N G /C O LLEC T IO N C H A R G E:
P O S SIB LE SA M PLE H A Z A R D S:
COM M ENTS:
SC C T'
BTS-nUMBeR:'"
CO NTRACT LAB:
IN IT .: DATE:
C O N T R A C T N O .(S ): W T T craru rrcw rr"
7 SAMPLES RECEIVED AFTER 2 :0 0 PM MON. - THURS. ANO 1 :0 0 P M ON FRIDAY WILL BE PROCESSED THE NEXT BUSINESS OA Y 17:00 A M - 3 :3 0 PMI.
' 'F O R LA B U S E O N LY II
R E JE C T E D [ )
REA SO N :
H O LD IN G T !M E ( )
SA M PLES V ER IFIC A TIO N
C Q N T A IN R S ( )
IM P R O P E R L Y S A M P L E D (
)
OTHR( )
IN ITIA L DATE.
I I L L I N U U I S H L U B V J I I . v^ U i . P a v I J . u U i l
| H t l U bV / ) f a -
d
0 0) n
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UUM M ANVJLUM M ANU / I V , J r
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DATETUM fc
-, ,,, f r
U A t< 1IM fc s / . , y , >c*< .> L
" ^ 'o A T iT T T M ^ r / O 'I T j
CHAIN-OF-CUSTODY RECORD
VC
ENVIRONMENTAL
& ANALYSIS REQUEST FORM
PWC ENVIRONMENTAL LABORATORY CODE 9 3 0 BLOG Z-140 9742 MARYLAND AVENUE NORFOLK, VA 2 3 5 1 1 - 3 0 9 5 PH: (7571445-8851 FAX: (75 7 )4 4 5-8 8 5 2
CLIENT INFORMATION
COMPANY/COMMAND: OT)U. I F ) Oj
CONTACT: 5 , VCJy
^
PHONE: ( e n f a s i -<a ^ X T :
J.O. : 1 ' % x a.g L
SIGNATURE: p, t.N - K l *>
PERMIT NO.:
CODE: FAX:
1YPE
G CRAB C F C O M P O S IT E, FLO W C T C O M P O S IT E, TIM E
M A T R IX
L -L IQ U ID
SW SW IP E
S -S O L ID
GS . GAS
*
S S - S E M I SO LID
P -P L A S T IC G L-G LA SS T . TEFLO N V.VOA
C O N T A IN E R
B-DAG
A A M B E R (g la ss)
C -C A R T R ID G E
T L - T E F L O N L IN E D LID
H -H E X A N E R IN S E D
P R E S E R V A T IV E
1 CO O L TO 4 C
5 - H C L , pH<2
2 H N 03, pH<2
6 . 0.003V. R a 2 S 2 0 3 , 4 C
3 H 2 S 0 4 , pH<2
7 . F IE L D F IL T E R
4 . N aO H, pN>12
8 . NONE
rj.'S'ACCU H D
iF
COMMENTS'
(TOR RUSH TURNAROUND S TA T RE AS O N UF.LOW)
S A -Y .P I: O IS P
L: ( ( R E T U R N T O C L I E N T ( ( D I S P O S A L G Y L A B
REGULATION APPLIED:
RCRA ( )
HRSD ( )
SDWA ( )
TSCA ( )
CWA (. ) PHOTO ( )
CAA ( ) OTHER ( )
l U 4-V XL.
S A M FLIN G /C O ILEC T IO N C H A R G E: P O S S IB LE SA M PLE H A 2A R O S: COM M ENTS:
CO N TRACT LA B:
:
---- pNIT.:
D ATE: "
I. C US TOMEn IS RESPONSIBLE TOR ALL CHARGES NECESSARY TOR THE PROCESSING A N O ^ N ^ ^ S l T o E ^ A M P ^ f l ^ M 'A LL CU SH S A M P L E S A R E S U B JE C T TO S U R C H A R G E .
C O N T R A C T N O .(S ):
'OFFiCTA'CVSETURCY----
7 CAMPLES RECEIVED AFTER 7 :0 0 PM MON. - THURS. AN D 1:00 PEI ON ERIOA Y WILL BE PROCESSED THE N E X T BUSINESS DA Y 17:00 A M 3 :3 0 PMh
' FO R LA B U SB O N LY!!
REJECTED( )
REASON:
H O LD IN G T IM E ! )
SA M P LE S VERIFICATION
C O N T A IN E R S ! )
IM P R O P E R L Y S A M P LE O f
)
O TH ER! )
firEORTTiTlST lED' U T [T F r n T o m s n E U t ) v :
o ,y
i l i ' CElRUUISNuU BTC S U H a tte # " -
[H tC u o y ^ r X T .A v
K b c u B r j g g r 7ZT
R EC TTB T
.-fn *ir.- ;rj^ iT F n jT 7 " '
"m n u T
A il
>j- , ' ' CUMPANV/UUMMANU" '" / ' J Y x ........C iv'"
CMPANY/LQMMAN.
' 'T ~ x'.-V
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"O O P -
M U M '1
IN IT IA I
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UAI klIIMEj[4<C 7 l 7 / 0
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PTTT
A :_ "
ftsc/d by :
^ ^ 7 / V l i 00
A PPE N D IX D-2 BNR Inhibition Pass-Through Acute Toxicity Test Results
60 ppm A FFF Pretreated with Defoamer AF9020
D-53
US00001234
D-54
US00001235
Coastal Bioanalysts Inc.
ODU/Oceana Acute Effluent Toxicity Tests
M ysidopsis bahia & Cyprinodon variegatus
Sample I.D. Feed Contract No. N00187-93-D-9748 , Delivery Order No. 0235 J O. No. 1912290, Item No. 0002BJ, 0002BH
PWCNo. 1367 11/2/97 1110 Sample No. JRA97-12376
Submitted to: Ms. Merrill Anderson-Ashcraft
Navy Public Works Center Code 930, Building Z-140
Norfolk, VA 23511
By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062
(S04) 642-0168
D-55
US00001236
O D I'O C KANA Sample I.D. Feed 11 :.V7 Sample 97-12376 Test ID: JR A 97I29
Coastal Bioanalysts Inc.
I. TEST RESULTS
% Effluent Concentration
0 6.3 12.5 25.0 500 100
4S-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acute Test Results
Mean (%) Organism Survival
M . bakia
C. variegatus
4 M 24-h 48-h
10*0 100
100 100
100 100 85 85
55 50
70 65
15 0 00 00
65 40 75 ' 30 50 0
12.5% 10.7-14.6 Spearman-Karber
,8.00
19.7% 14.0-26.8
Probit 5.08
Acute Test Information:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
M . b a h a Test 48-h Static Survival 11/3/97 1605 11/5/97 1540 CBI stock cultures 10/31/97 20 g/kg 20.0-20.6C No N/A
s
C variegatus Test 48-h Static Survival 11/3/97 1620 11/5/97 1535 Aquatic Bio Systems 10/30/97 20 g/kg 20.0-20.6C No N/A
'D.O. concentration decreased to slightly less than 4.0 mg/1 in 100% concentrations on day 2 of the sheepshead test, the drop was unanticipated and probably caused by a bacterial bloom (solutions turned milky color overnight). Low D.O. was not the primary cause of toxicity as indicated by low survival in concentrations with adequate D.O. concentration.
D-56
US00001237
ODt.'-OCKANA Samp! I D. Food I M 9 7 Sample 97-12376 Test ID: JRA97I29
Coastal Bioanalysts Inc.
Sample Collection pate/Time
11/2/97 1110
Sample Use . Dates/Times
11/3/97 1605/16203
Aeration2 Rate/Tima
n/a
Pretreatment/ Adjustment
HWM Salts
2Aeration often necessary to reduce sample oxygen concentration to 100% saturation after warming. 3_V/. bahia/C. variegatus test time.
Table 2. Effluent and Dilution Water 4 Chemical & Physical Characteristics (Mean Value (Standard Deviation))
Parameter
Temperature (C)
Salinirv (g/kg)
Conductivity (uMHOS/cm)
pH
Dissolved Oxygen (mg/1)
Total Hardness (mg/1 as CaCOj)
Alkalinity (mg/1 as CaC03)
Total Chlorine (mg/1)
n h 3-n (mg/1)
Initial Effluent
3.4 (...)
N/A
1960 (._)
7.10 (-)
10.7 (--)
143 (-)
372 (-)
<D.L. (-)
32.5 (-)
Adjusted Effluent 20.6 (--) 20 (...)
N/A
7.32 (-) 7.9 (-) N/A ' s N/A
N.D.
N.D.
Dilution W ater 20.5 ' (...) 20 (--) N/A
7.96 (--) 7.9 (-) N/A
N/A
N.D.
N.D.
4Effluent parameters measured upon receipt of sample in lab and after adjustment on day of use. Dilution water (Wimex hw-Marincmix in ASTM Type I deionized water) values measured after preparation of diluent on day of use.
D-57
US00001238
O D I' OCEANA Sample I D. Feed 11 2 97 Sample 97-12376 Tesi ID: JR ;\97129
Coastal Bioanalysts Inc.
n . WATER QUALITY
Parameter Temperature
(C) DO. (mg/1) pH
Salinity (g/kg)
Parameter Temperature
(C) D.O. (mg/1) pH
Salinity (g/kg)
Table 3. Water Quality Summary
Control
20.4 (0.1)
7.8 (0.2)
7.78 (0.16)
20 : (0)
M . baha Test
Mean Value (Standard Deviation)
6 3%
R
20.5 (0.2)
7.5 (0.4)
7.56 (0.26)
20 (0)
12 5%
20.6 (0.1)
7.1 (0.7)
7.56 (0.25)
20 (0)
25%
20.5 (0.1)
6.3 (15)
7.57 (0.20)
20 (0)
50%
20.5 (0.1)
7.0 (1.4)
7.51 (0.01)
20 (0)
100%
20.5 (0.1)
6.8 (IT)
7.58 (0.37)
20 (0)
Coniil
20.5 (0.1)
7.8 (0.1)
7.81 (0.17)
20 (0)
C. variegatus Test
Mean Value (Standard Deviation)
o%
20.6 (0.1)
7.7 (0.3)
7.44 (0.35)
20 (0)
12.2%
20.6 (0.1)
7.5 (0.5)
7.43 (0.30)
20 (0)
25%
20.5 (0.1) * 7.0 (0.8)
7.50 (0.22)
20 (0)
20%
20.5 (0.1)
6.6 (1.2)
7.49 (0.21)
20 (0) .
100%
20.5 (0.1)
5.7 (2.1)
7.61 (0.34)
20 (0)
/ D-58
US00001239
O D I' O C E A N A S am p le I .D . Feed
1 1 1 9 7 Sam p le 9 7 -1 2 3 7 6 To st ID : JR A 9 7 1 2 9
HI. OUTFALL DATA SUMMARY
Coastal Bioanalysts Inc.
Test Date
9/30/97 11/3/97*
Table 4. Cumulative Data Summary - Acute & Chronic Tests (Tests conducted on this outfall by CBI)
Invertebrate
Acute Tests
Chronic Tests
LC?o T.ll.
M.OE.C T.ll.
Vertebrate
Acute Tests
Chronic Tests
LC50 T.U.
NOEC T.U.
8.1% 12.35 12.5% 8.00
N/A N/A N/A N/A
3.5% 28.57 19.7% 5.08
N/A N/A N/A N/A
Results submitted with this report
IV. QUALITY ASSURANCE
M. bah ia - Acute Reference Test Dates:Reference Toxicant: Test Concentrations: Control Survival; LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
C variegatu s - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
CBI Stock Cultures 10/15/97-10/17/97 KC1 (Sigma "Ultra" Lot#76H10391) 343, 490, 700, 1000 mg/1 100% 574.2 mg/1 (540.9-609.5) 612.1 mg/1 (488.3-735.9)
Aquatic Bio Systefns, Inc. (same batch as test) 11/3/97-11/5/97 KC1 (Sigma "Ultra" Lot#76H10391) 432, 720, 1200, 2000 mg/1 100% 1200.0 mg/1 (1070.5-1345.2) 1074.9 mg/1 (891.2-1258.6)
D-59
US00001240
Ou
Date;of, ' Lsa
2/12/97 <6.25 2/28/97 Invalid 3/12/97 17.7 3/20/97 19.5 3/26/97 33 9/30/97 8.1 11/3/97 12.5
CUMULATIVE DATA SUMMARY NPDES Permit #: N /A Oceana - ODU Feed S.
Invertebrate..;:.; L 'C S O fe S Y lfS ^ NOE%> 31
35, 52 35 34 3.5 19.7
VrteBrafej'NOEC%V '
Supplement
D-60
US00001241
C O A ST A L B IO A N a LY STS. INC. N PD E S S T A T IC A C U TE/SA LTW A TER.
(F orm AMB*4tt)
\ Effluent
C ontrol
6 .2 5 1 2 .5 25
1. 0 .
Oay 1 L ive
JOC -l
iC-2 ()
M 1 f\
1 h1-2 ip2-1
52 -2
A3-1 63 - 2
0y?
9 Live
Final 1 Survival
\r>_____________
>0
|oc
_ i J __________________
M ___________________
10C-
________________
r -->
3
o
5c O
4-1 5 0 4-2
5-1 100
5-2 O bserver
iiim :
O
o
o O C-N
_ Q _____________________
. __ _______________
Q
trH
l5VC>
0
M v sid o p sis b a lia
CBI
Faeno poor to lest: A rtem a ^ X
w r) f * . I f t /d Other
F eed rq during tear. Not fed O
Artema I X . j " 0 th ^
C h a r t w . : 3 0 0 n4 J 3
SO O ^O
1 0 0 0 irt
O tfw r
( H in :
2 0 0 it4 ^ 3
2S0P4Q
tranal no o m a r s v r e oer feolicate:
/^
5 0 0 (i4
O tte r
cpN c L i e d n t e c
P h o to p v n o d 16L :80 ^ 3
O t f - r __________ R a n d j i i m i c w t --n 4 f n O - ? 6 -- t
d4
ni
SET U P 0 i _ i _ L C 3 Z i 3 ___________ T m V h m r M M
___________
IL&*)T xn* A n n u ls A d * d _
s.t U p & r _ r ____________
IMSTRUMENTATION USED:
A ?S t e m T h e m o m e t e c N u m b e r
TS1 0 .0 . M e te r # 1 ( 9 6 8 0 1 4 4 7 S ) C P D q tsa ra e pH M erer (0 9 5 0 0 5 6 3 )
S e n o rw e H tS O P B e l (40020093) Y S SOT 3 3 M e ter (H 8 0 1 6 7 9 2 )
S Y S 0 .0 . M eter 2 ( S f i 1 * 0 2 6 3 2 9 ) C o m q 2 4 0 pH M eter ( 9 6 2 6 8 ) SP P o r t * M eter (8 5 2 3 6 0 ) E l Specter ATC (W n e to im 'u f (*366)
O tW
OayO
0y I
Oay2
C <Q S A . V QD-y
T 1 r) G (o
E M
2 3 P , Co W c Qi> ic
p 3 P d .T
3 (r
4 30b
5 <3sG.(s 34-V
c X 9(n
7.1, 9
7. s~/
2 ?.DS
3p H
m m
4 7--SW A .s 'c
5 7~.
7- SV --
C 7- 1 7 -f
7 -ff ?.. / .
7 U7i
0.0. 2 7 - f (_
(ttgfl) 3 7 - 1 Co .A
4 Ci '6
S . --.
5--
sc A1 L
2 N3
4
5 Radicate9
Irutiels:
at,
AA Aa
r> Ad
( lif
36 A m -- AA 7h
. M ___
3 A ... _ 3 j2___ --
A6 . -- 3 _x_
A // Cr/f
NOTES
V J 9 c^ C-fl>\ac).( t>r\ 3 V \f (
7 r --
40
5onA
T . C-- H ------------------------------------
Quafcry A s s u m e :
A s Q u r f r y A s s u m e o f f i c e tv t b s t e s t I c e r t i f y t h a t 0 0 * * g n ifie an l
c k v w lim s fro m a c c e p te d p ro to co l o c c u re d dunng rovtuto in sp ectio n s (u n le s s o th e rw is e n o te d b e lo w ) a n d th a t th * <&ta a c c irttW y r e fle c t th e te st v it w as f fo m d by C8L
Comtartfs
______________ ______
LL i~ )h n
TEST I D.: T r t r t D-61
US00001242
TRIMMED SPEARMAN-KARBER METHOD. V E R SIO N 1 . 5
DATE: . 11/3/97 TOXICANT : FEED STOCK SPECIES: M BAHIA
RAW DATA: Concentration
.00 6.25 12.50 25.00 50.00 100.00
TEST NUMBER: JRA97129
DURATION:
Number Exposed
20 20 20 . 20 20 20
Mortaliti
0 0 ' 10 20 20 20
SPEARMAN-KARBER TRIM:
-00 %
FpFARMAN-KARBER ESTIMATES :
LC50:
95% LOWER CONFIDENCE:
95% UPPER CONFIDENCE:
12.50 10.71 14.60
48 H
D-62
US00001243
Ci JA.STAL UK>AN,\LYSTS. INC. NPDES STATIC ACUTE/SALTWa TER
(Form ACV4S)
% Affluent
Day 1 I.D.
1 Live
Day 2
9 Lrva
C-l C-2 M 6.25 1-2 2-1 12.5 2-2 3-1 2S 3-2 4-1 50 4-2
5-1 100 5-2
Observer Tim:
" o '6 1
V% ...
Isj
u
____________
%
? y V
S'
1 o
3o
lb O P
Final X Survival e rs
Y t> 5 0
Smart: Cyprinodon variegati____________Soum:_______ 691- & & S
chOit^Tur;_ / 0 / 3 0 / U __________________________________
Acclimationwater HWMASW ^3 Other:
SahrstY:<-^ f\
Accliraiion Temp. (range):
Q ( Q_______ to D . \ P
Feedngpoor totest: Anema ^ ____ /d Othar_________
Feengduringtest: Nor fad 0 Aftenia_______/d Othar_
Chamber sue: ^00 ^ 3
SOOrrt
1 000 iw | Q O ther
T ail vohana: 200 rri Q
250 tri Q
500 tri J S
Othar
trattai no. orgarasms par rapficau:
fo
No. n p ticac : Q _______
ftn to p e n o d 16L.80 ^
O ther
Randpm gitjon template nc^ "
rH
ni
SET UP: 0 * a _ I ( % I
r-- W KAdhd _ l 5
L>
(^t Cl ^ P-K-fTima Animats Added
Sal Up By_
INSTRUMENTATION USE0:
Sum Thanrxtraltr Nrfrtac / }
T S 0 .0 . M . u r # I (SNE8014475) ! CP OtgiMffi* jfl M eter (D9SOOS63)
I S m o n ta RI 60P Balance (40020093)
rSISC T 33 Motor (H 80I6792)
I Y S D.O. M eter # 2 (SW 1A C26329)
I Coming 2 4 0 H M eier ( 9 6 2 6 8 )
O SP P o rta Motor (652380)
S i o t n t ATC R ofnctotm tor (A366)
Other
C . -0 f> S
.. 3 0 \r
So. .t
<f
2 -7 o ( - - S i s "
Vt
3
' o nu )
3 o .v
- 3 d fc
4 3oU
.S
)p .r
5 HP
c 7.YU / V
-3D -V T T -- rr--
t i L* 3
1 7 - - <vT `-3 > - 3 3 ^ ? 3 7 .
2 7.3
pH
3 7 . `4 s - i , 9
4 7.J 7 5 7 3 ^ 7.'53
3.7C H ft
! t 7J . 9 7 y
0.0. 2 1 : `i
tmgrt)
3
7 -1
7 -6
Ir-3
4 1. 0
b -/
5 -;*
5 7b
r .3
3 -f
SC
A
L I2
H3
(gfltql
4
5
cat 9
3Q of)
3o
r0 fi
J? f>
H
)
O
H6
Ht
7)C-
u>
3 i-.
Zo IO
"7 <3
Ab
3a
r7 6.
Q
nah:
Wf
L-
NOTES:
c~> y K v r r - > j V - | 5
&
(kaliry Assurance A s Qualiry Assuranca oHicar for this tast 1certify that no s^nif< ant (Mvianore from accapted protocol occurred dunng routine inspectors (uniats othanmiM noted baJow) and that the data accurately rvflact tha te s t as <t w as performed by C31. Comnena
'{ v - J r v J )
ligMU
c0
'ih h i
0m
TEST I D.: `f i t / 9 7 > J ? D-63
US00001244
EPA PROBIT ANALYSIS PROGRAM USED FOR CALCULATING LC/EC VALUES
Version 1.5
JRA97129 C VARIEGATUS
Cone.
Number Exposed
6.2500 12.5000 25.0000 50.0000 100.0000
20 20 20 20 20
Number Resp.
3 7 12 14 20
Observed Proportion Responding
0.1500 0.3500 0.6000 0.7000 1.0000
Proportion Responding Adjusted for
Controls
0.1500 0.3500 0.6000 0.7000 1.0000
Chi - Square for Heterogeneity (calculated) = 2.978
Chi - Square for Heterogeneity
(tabular value at 0.05 level)
= 7.815
JRA97129 C VARIEGATUS
Estimated LC/EC Values and Confidence Limits
Point
Exposure Cone.
95% Confidence Limits
Lower
Upper
LC/EC 1.00 LC/EC 50.00
1.679 19.695
0.400 14.003
3.437 26.830
8
D-64
US00001245
'COASTAL BIOANALYSTS INC. EFFLUENT/DELUTION WAT E R D A T A (Form e k d l o is s )
INITIAL (ARRIVAL) EFFLUENT CHARACTERISTICS:
Sample Date I'IQm D
11 11 1
Temp. (C) 13.V 1 1 i
111
Conduct. (uMHOS] 1
1
I
I
11
|'
Salinity (g/kg) | I
I
I
I
II
PH IT-. i ii 1
1111
Diss. Oxygen (mg/1) 1( 0 .} 1 1 1 1 1 1
Tot. Chlorine (mg/i; 1 1 1 1 1 1 1
NH3-N (mg/I) 1(Yr-2 L3^.S" 1 1 1 1 1
Hard (mg/1 CaC03) !| V3 1 1 t
11
1
Alkln (mg/1 CaC03) 1 3 ? ^ 1 1 1 I l 1
Color/Appearance1 K 7 1 . 1 1 1 1 1
Initials
1 Irti 1
1
1
1
1
1
1 C-Clear O-Opaque Y-Yellow B-Brown BI-BIack G-Green T-Turbid SI -Solids (S-Slight M-Modcrate V-Very Much)
EFFLUENT ADJUSTMENT/POST-ADJUSTMENT MEASUREMENTS:
ACUTE
Date | 1 1 1 1 liv ra i-
Temp. (C.) |
1 1 1 1 1 1 Il S)c.U,
Salinity (g/kg) |
1 1 1 1 1 Do
D O. (mg/1) |
1 1 1 1 1 1 117.1
Aeration Rate | Aeration Time (min) | Adjust. D O. (mg/1) |
pH |
1 1 1 1 1 1 II --
1 1 1 1 1 1 II -
1 111 1
ID
111
1 1 II 7-30
Tot Chlorine (mg/1) |
Initials
|
1 111 1
llk Q.__
1 1 1 1 1 1 II
DILUTION WATER CHARACTERISTICS:
DMW:
H W M v/
acute
Date |
1 1 1 1 1 1 Il 1//I/ l
Temp. (C) | 1 1 1 1 1 1 n o
Conduct. (uMHOS) |
1
1 1 IN .fl
Salinity (g/kg) | 1 1 1 1 1 1 ID u
pH | 1 1
1 1 1 in -iL
Diss. Oxygen (mg/1) |
1
1 1 1 I 'M
Hard (mg/1 CaC03) |
1
1
1
II N/.A
Alkln (mg/1 CaC03) | 1 1 1 I 1 1 IIA/A
Initials
|
1
11
1 1 D -h _
EFFLUENT IP: ^ t l l
11
TEST ID: T ft-f)
D-65
US00001246
Toxicity Test Sample Chain of Custody (Please complete ail information)
F a c i l i t y .... Q \ c J L t 7 0 v n j y i ( o n
Addre-s-s, k )o ^ \ W . VA-
U n t^ r C i- h ^
J
'J0i / J i
- ili
County----------------------------- _____
Pipe/OutfalL/Location .________________ _
NPDES#-- ;---------------------______
Instrcam Waste Cone ____________________
Sample'collected by (print&sign)J J o ^ Q n
Type of sam ple_^_(Grab):
Date
J -/n
Affiliation DDU Time U ' >0 ( X * \
L^U ^
-- I I __(Composite): From Date ~
Time --______
To Date_____ -- Subsamples comprising composite:
Time
--______
Number__________ * Frequency of collection_________ Volume__________ 'Temperature of sample in sample collection device 0 Q j
Final temperature of effluent at sample collection point o ? 2 .% c Q s
'Is sample collection device chilled?
Is sample packed on ice for shipment?
'It is required that all samples remain at 0 - 4C during collection period and shipment for data to be accepted by the appropriate Regulatory Agency fDo not freeze!)
Is the sample chlorinated? ^
aechlorinated?
[f so, how?
Permit with interim chlorine limit?.
If yes - limit (mg/L)_
Field pH ( o . ( g %
Field Total Residual Chlorine
Comments/Sample description o i U 'te fA
<3>~fc>CVC
___________
Type of test(s) to be performed
p . C cU pY inCdO fl
(Specify organisms)
________ ' .________
Method of shipment. O e W e rtj <9CU______
Print & Sign Names
Relinquished bv CV. \C * \ ~ UA/LiL Date
Received bv 0
Date
Relinquished bv O .
Date
Received bv
Date
W /S /47
iif'S h ')
i\h i?a '
Time \ 0 '- C O a r t Time / o ? s
Time // ?
Time \ / A ? ri
FOR REED LAB USE ONLY
jra * 9 7 W 3 7 C
Arrival Temperature__________
Color_____
_____ Odor______
P H ______________
DO (mg/L)_
Conductivity (umhos/cm)_
Salinity (ppt)_
TRC (mg/L)_ _____ Method_________
On ice? . Solids. @_____
D-66
*/ 5 /1 0 /9 4
US00001247
uul,H
V
13
7o
D-67
US00001248
O DC OCEANA Sample I D. Mixed Lq I 1/2.97 Sample 97-12377 Test ID: JRA97130
Coastal Bioanalysts Inc.
I. TEST RESULTS
'
% Effluent Concentration
0
6.3 12.5 25.0 50 0 100
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acute Test Results
Mean (%) Organism Survival
M. bahia
C. variegatus
24-h 48-h
24-h
48-h
100 100
100 100
100 100
100 100
100 100
100 100
100 100
100 100
100 100 100 100
100 100 100 100
>100% N/A N/A <1.00
>100% N/A N/A <1.00
.
Acute Test Information:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
M. bah ia Test
48-h Static
Survival
8
11/3/97 1610
11/5/97 1555
CBI stock cultures
10/31/97
20 g/kg
20.0-20.6C
No
N/A
C variegatus Test 48-h Static Survival 11/3/97 1625 11/5/97 1545 Aquatic Bio Systems, Inc. 10/30/97 20 g/kg 20.0-20.6C No N/A
1Aeration nccessniy when effluent oxygen demand results in dissolved oxygen concentrations < 4 .0 mg/1.
D-68
US00001249
ODl : f>CF.ANA Sample I.D. Mixed Lq II-Z 9 7 Sample 97-12377 Test ID: JRA97I30
Coastal Bioanalysts Inc.
Sample Collection Date/Time
11/2/97 1900
Sample se Dates/Times
11/3/97 1610/I6253
Aeration2 Rats/Iime
n/a
Pretreatment/ Adjustment
HWM Salts
2Aeration often necessary to reduce sample oxygen concentration to 100% saturation after warming. 3.V/. btihia'C. variegatus test time.
*
Table 2. Effluent and Dilution W ater4 Chemical & Physical Characteristics (Mean Value (Standard Deviation))
Parameter
Temperature (C)
Salinity (g/kg)
Conductivity (uMHOS/cm)
pH
Dissolved Oxygen (mg/1)
Total Hardness (mg/1 as CaCOj)
Alkalinity (mg/1 as CaCOj)
Total Chlorine (mg/1)
n h 3-n (mg/1)
Initial Effluent
3.2 (-)
N/A
1436 (-)
8.45 (-)
11.2 (-)
127 (-)
273 (-)
<D.L. (-)
1.5 (--)
Adjusted Effluent 20.6 (-) 20 (-) . N/A
8.12 (-) 8.0 (-) N/A * N/A
N.D.
N.D.
Dilution W ater 20.5 (--) 20
(...)
N/A
7.96 (-) 7.9 (-) N/A
N/A
< N.D.
N.D.
4ElTIueni parameters measured upon receipt of sample in lab and after adjustment on day o f use. Dilution water (Wimex hw-Mnrincmix in ASTM Type 1deionized water) values measured after preparation of diluent on day of use.
D-69
US00001250
ODU<X'K.\NA S imple I D. Mixed Li| 11.297 Sample 97-12377 Test ID: JR.A97130
II. WATER QUALITY
Coastal Bioanalysts Inc.
Parameter Temperature
(C) DO. (mg/1) pH
Salini ry (gAg)
Parameter Temperature
(C) DO. (mgrt) pH
Salinity (gAg)
Contrl
20.4 (0.1)
7.9 (0.1)
7.79 (0.16)
20 (0)
Table3. Water Quality Summary
M. baha Test
Mean Value (Standard Deviation)
12.5%
25%
50%
20.5 (0.2)
20.6 (0.3)
20.6 (0.2)
20.6 (0.3)
7.8 (0.1)
7.8 (0.1)
7.7 (0.2)
7.7 (0.3)
7.86 (0.12)
7.89 (0.09)
7.98 (0.04)
8.11 (0.04)
20 20 20 20 (0) (0) (0) (0)
100%
20.6 (0.3)
7.5 (0.4)
8.24 (0.11)
20 (0)
Control
20.4 (0.1)
.
7.8 (0.1)
7.82 (0.17)
20 (0)
C. variegatus Test
Mean Value (Standard Deviation)
6 3%
12.5%
25%
50%
20.6 (0.1)
7.7 (0.2)
20.7 (0.2)
7.7 (0.2)
20.7 . (0.2)
7.7 (0.2)
20.7 (0.3)
7.7 (0.2)
7.87 (0.11)
7.91 (0.08)
7.97 (0.04)
8.09 (0.03)
20 20 20 20 (0) (0) (0) (0)
100%
20.5 (0.3)
7.6 (0.3)
8.15 (0.09)
20 (0)
D-70
US00001251
ODI ' OCE.VNA Simple I.D. Mixed | II 2.97 Sample 97-1 23" 7 Tesi 1D :JR .\9M 30
III. OUTFALL DATA SUMMARY
Coastal Bioanalysts Inc.
Test Date
9/30/97 11/3/97*
T able 4. Cum ulative Data Sum m ary - Acute & C hronic Tests (Tests conducted on this outfall by CBI)
Invertebrate
Acute Tests
Chronic Tests
LC50 T IL
NOKC XJL.
>100% <1.00 >100% <1.00
N/A N/A N/A N/A
Vertebrate
Acute Tests
Chronic Tests
LC50 T IL
NOEC T.U.
>100% <1.00 >100% <1.00
N/A N/A N/A N/A
` Results submitted with this report
IV. QUALITY ASSURANCE
M. bahia - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
C. variegatus - Acute
Reference Test Dates:
Reference Toxicant:
Test Concentrations:
Control Survival:
.
LC50 Reference test (95% C.L.):
Control Chart LC50 (95% C.L.):
CBI Stock Cultures 10/15/97-10/17/97 KC1 (Sigma "Ultra" Lot#76H10391) 343, 490, 700, 1000 mg/1
100%
574.2 mg/1 (540.9-609.5) 612.1 mg/1 (488.3-735.9)
Aquatic Bio Systehns, Inc. (same batch as test) 11/3/97-11/5/97 KC1 (Sigma "Ultra" Lot#76H 10391) 432, 720, 1200, 2000 mg/1 100% 1200.0 mg/1 (1070.5-1345.2)
1074.9 mg/1 (8 9 1 .2 -1 2 5 8 .6 )
D-71
US00001252
'n
CUMULATIVE DATA SUMMARY NPDES Permit #: N/A Oceana - ODU Mix L. R.R.
Date ofrjf Iavertebrate^--7 - Tsti
2/12/97 >100 2/28/97 Invalid 3/12/97 >100 3/20/97 >100 3/26/97 >100 9/30/97 >100 11/3/97 >100
V e r t e b r a t- e ' A^-g Invertebrate , ' . Tr Vertebrate N P E C ^ - ' : ';.' n d e S%?;
>100
>100
>100 >100
>100 >100 >100
-"*Vi
Supplement D-72
US00001253
COASTAL 8HMNALVSTS. IN C NPDES STATIC ACUTE/SALTWATER
(Form AM B4K)
\ Effluent
t.D.
Day 1 live
6.25 ' 12.5 25 50
C- C-2 M 1-2 2-1 2-2 3-1 32 4-
4-2
1
lo lo
1\ 10 I0
i>
1b
\Q
lb
5-1 ( b
5-2 Observer
1
C-M
Tiros:
/b i
Day 2 # Uve
(0 10
'0 tp
f(i
i< *
10
u
1\
io
F\n %
Survival
,O Q
VO t5
i 0 <\
vC G vO O ;1 ^O W It
1 L .J
'
V eo*' M ysidopsis bahia
Hatch OatefTirra. f /3 /
Acchnwtioft w a te r HWM ASW
Sowce:
CBl
Other
7 "Silfiry .
Acchmation Te r ra (range):
^Feedng pnor to test: A r te n a
X
l O ther
O f e e in g owing test: Not fed
Artema \ X /d
Other.
Chntf sue: 300 iti
[3 50 0 iti
1000 tri
O tf-:
3I . i n h n 2 0 0 r t
2 5 0 " !
S 0 0 fri
Othar
Intvaf no. ortarosna oar radicate:
/&
/ONa. rocArarB*
Photopeno<t 16L:80
O ther
Randomston tam dete n o ^
SETUP: Dm* I l f 3 / 4 T
f HT r a W i u r A l M
b
T u ra A iM tiltA iJM | L> f
S .iU p B r O'ff
fri fri
'
INSTRUMENTATION USED:
Stem Thartm rm ar NurtCar
YS 0 .0 . IfU tw I 1 (SNE8014475) j CP D iy a m . M*t*f (09500563)
S in o n R1S0P B i l u n (40020093) YS SCT 33 M ottr(H 80)6792)
;1 fYS D.Q. Mm v 2 (SN91AQ26329) L [Ctmxg 2 4 0 pH Uw (9 6 2 6 8 )
LJ SF P o rt* H 2 Motor (952360)
Q S fo ra n ATC Rifnclorm tor (4366)
Other
DayO___ PayI
Day2
c
r ' "S
23.y
D . l|
------ -- -P "
1 T u .7
-i
f^ p 3
2 ^ > 'I n i o 3
3 0 .X
4
5 X C
7 - H o
r
^ P .(o
^o z . -
T -.P V
1
7 ."H
7 .P J
2 I f , O 7 . f t
3 ? ./> o ? -1 ?
4 2 , b?
5 x .n
C
7 'j
l-> 1
ff-r^ O? 77
7 -7
3 -fS " ris '
3 - .? -
.
2 7 -1
3 M
4 m
5
>> 77
7 .7 7 .5 "
C
2 3 4
5
cate i
fielt: NOTES:
c3 P
7 1) (J
-0
3- A 3 . />
-?< i ,^ p
7A
A0
a /) /
<7 H A
.
i i f ________J i l ________ ( ^ 7
QioAty A u m n c a :
As Qiery Assurance officer for r h s test I certify that no ugrvficant (kvislm m from accepted protocol o c c v rtd dm ng routina inspections (unless otherwise noted betow) and th at the a u accurately ro lled the la s t B it was parformad by C8I.
CortWBiti
C) A - V !V vi^ P .J^
S fM W I
-'
^ n / 'l fa'?
0*
TEST ID : 7 / 1 4 4 7 H f. D-73
US00001254
COASTAI. UIOANALYSTS. INC. NPDES STATIC ACUTESALTWATER
(Form ACV48)
\ Effluent
Day 1 I.D. L ive
Control
6.25 12.5 25 so
C-1 \ 6 C-2 K
M
1-2 >& 2-1 1 i 2-2 10 3-1 < o
3-2 - 0
4-1 I D 4-2 1b
5-1 ' 0
100 5-2 1 f,
Observer
C -H
T in:
Day 2 Irv*
to ( 11 ro ____ 6 to ip 1 L-
' i
1h \D 10 C-H /< y r
Final \
Surviva) i o
\d o
t OC
j oo
1o o
,0 4
C vorinodon v a rie aa tu s
Hatch Date/Ttn: ^ & { ' 3 0 / ^ ^
Acrhrratron wair: HWW ASW [ ]
O ther
Source:
eei- r t - 6 S
SMimry 2 \
Acchrranon Tem a (ranocl:
* 3
F e e in g prior to test: A n e m a ^ j X
to c i 0 .1 A /d O ther
F e e in g tiring test: Not fed
Anema
li Other
Chamber size:- 300 rrf [ 3
5 0 0 rri
1000 irt 1 2 O ther
T -tv o l TM : 2 0 0 ni Q 3
2S 0niO
500 ni J 2 O ther
Irattel no. oro a n sn s oar ra d ic a te
(O
No. r e d ic e f g
id id
Photopeno 161:80
O ther
Randpraration te m ia te no. (_$
^
SE T U P Dtu l I ? ^ / 4 " ? - _ T i r W iw A d M ( S i 9 3
Tins Amrrsh Adrkd_ {
*2 ____ Set Up By _ { j j j .
INSTRUMENTATIONUSED:
Sl*m TNmranM pr Hjfrtar. / t I______
1 3 Y S D.O. M .M r I I (SNE601447S} 5 CP Oi^ m a m pH M .tr (D9S00S63)
H Sanonus Rt SOP B a l m 140020093!
3 YS SCT 33 Malar (H 80IS792)
gY a 0 .0 . Malar 2 [S tlA 0 2 6 3 2 9 ) C oning 24 0 pH M alar ISN526S)
SP P o r ta l M alar (8S2360I Sparlao ATC Rafiactonatar IA3S6)
Oihar______________________________
oP a y
Pay 1
Day 2
C a o .r
0 i>. v
26 ^
r
1 3 b2
P 0 .T-
E 2
M
3 p
4 2 4 ,?
3 -6 .4
y
5 . ! u
c
3 . 7 La
7 .U7 i
l
fH 2 f t . D 7 - W
3 ? . 0 s t2 -. ? C o ?
4 './ ^
5 ff. P ,
c
1
S .io ...
0.0.
(ntfl)
2 T -f
3
4
V9
V <r
'X .S
5
sC
A1 L 12 N3
4
5 Radicate
Infiali'
NOTES
? ? < 5 t\
t 3 />
2 Ds
34 0
r ) i>
<2 4
S f?
2n
< ( \
o? Ip
<2 0
ci 0
an
o>
2
13
_______
____ _ t z i ______
Quality Assurance: As Quality Assurancs officer lor this test I certify that no significant dMtatwns from accepted protocol occurred during routine inspections (unfats otherwise noted beta} end that the A ta accurately reflect the te s t as it wes pedom vd by C81. Comments____________________________________________
P S tr ife tfiw i
// - n / c t n Dm *
TEST ID.:
D-74
O /V i
US00001255
CHAIN-OF-CUSTODY RECORD
CLIENT INFORMATION
m>
E N V IR O N M E N TA L
& ANALYSIS REQUEST FORM
PWC ENVIRONMENTAL LABORATORY CODE 930 BLDG Z -140 9742 MARYLAND AVENUE NORFOLK, VA 2 3 5 1 1 - 3 0 9 5 ' PH: 1757)445-8851 F A X : (757)445-8852
C O M P A N Y /C O M M A N D: j Q i f l''0 r ________C O D E :
CONTACT
PHONE M S S i l EXT:
J.O-: I Tf A 5L'l D ,,
FAX:
S IG N A T U R E : o . v "-P, I
P ER M IT NO.:
LAB USE ONLY
Sampu
lin e it e m n SAMPLE NO SAMPLE ID/LOCATION
lA/iH
ooo'cA B
6 O A B T
m3 3 ( 6
n -w n , F * * A x 7 Yu le . ll'IZ T n prvi/cX Rtft-M-t.. (fc'ni.Ac
SUM Stop SUM Stop
1 7 -/A i? V
SIAM
B a f lF r r >- v C < ''''y stop
R "(-/A i/?
SIAM
I ^ B < 5 - I lf T r V I b f i i A i i f r Stop
'I7 -/A 3 7 D
SIAM
I3 ( 3 . fc 'K
i A fTP f p y-ia s S (
/ T 1 . - C o n Ky o I
stop SIAM stop
13, I*
r
ch - / d 7> s x Coarto \
SUM stop
OK 0A1E
h
At IH
M D '/oo M /< f Ob'OO o '/iF .*>. '3` i S3 -
ITPE SAHPtiO M
G L IT
1 _ ii_
_L
MAIRIL L
Of SAMPLES PRSfMIlVE /(OHIAIHES
ANALYSIS
FIELD READINGS pH UHPfRAlURE OlHEft
/ U SL l C : ......................................................................................
Ah ^
Ah
'l;n A /- ' '
7. y
A Oh
9, fa!
a h9
PAESfAvAlk VUlflfDti
yy
> F-ri
G -GRAB CF-COMPOSITE, FLOW
CT - COMPOSITE, TIME
M A ' RIX
L-LIQUID S SOLID
SW - SWIPE
GS-GAS SS - SEMI SOLID
P PLASTIC GL - GLASS T-TEFLON V - VOA
" " c i 3N TAIN ER
B BAG
A - AMBER(glast)
C - CARTRIDGE
T L - TEFLON LINED LID
H HEXANE RINSED
p ffs E R VATIVE
1 COOL TO 4 C
5 HCL, pH<2
2 - HN03, pH<2
6 - 0.008V. Na2S203, 4 C
3 * H2S04, pH<2
7-
FIELD FILTER
4 -NaOH, pH>12
8-
NONE
TUNAOUHD COMMENTS:
(FO R RU SH TURNAROUND S T A T E R EA SO N BELO W ) "
R E G U LA TIO N APPLIED:
RCRA ( )
HRSD( )
S D W A ( ) ... T S C A { )
CWA ( ) P H O TO ( )
CAA ( ) OTHER ( )
SAMPLING/COLLECTION CHARGE: POSSIBLE SAMPLE HAZARDS: COMMENTS:
SAMPLE DISPOSAL: ( (RETURN TO CLIENT ( (D IS P O S A L BY LAB
I C U S T O M E R IS R E S P O N S IB L E F O R A L L C H A R G E S N E C E S S A R Y F O R T H E P R O C E S S IN G A N D A N A L Y S IS O F S A M P L E S i. ALL RUSH SAMPLES ARE SUBJECT TO SURCHARGE
D.O. NUMBER: CONTRACT LAB:
INlT.: DATE:
CONTRACT NO.(S): 1OFFICIAVUSEONLY
j S A M P L E S R E C E IV E D A F T E R 3 : 0 0 P M M O N . T H U R S . A N D 1 : 0 0 P M O N F R I D A Y W IL L B E P R O C E S S E D T H E N E X T B U S IN E S S D A Y 1 7 : 0 0 A M - 3 : 3 0 P M ).
" FOR LAB USE ONLYII REASON: HOLDING TlME( )
SAMPLES VERIFICATION
CONTAINERS! )
IMPROPERLY SAMPLED! )
OTHER( )
INITIAL DATE
ra nilN U U IS H bU ?: im iN U U IS H b L ) 'U T " rREUNUUISHbU BVTM 1t J L i jn n ig H r n HY'
\ / IU -L -- - d L aL - ..................
........ JHEC'ITBYT ll-ibC'D Y:
" " "HbO'D 'BT7 IK b ' Y :
N "i>
Us,
- -COMPANY/CUMMANU'" CUMFTOWCOMM'ANU" OUMPANY/OUMMANL): L'UMPANY/CUMMANll.
p. n (1- A
"" A b /llM b :,,;//? ) n X
UA1hi 1I M b : /X3C,
U A lb/IIM b:
UAIb/IIM L:
in r-'
US00001256
R s J i> r
*4 -
R tv o ^ .
H -S - O
W ? /J ^
9
D-76
US00001257
Toxicity Test Sample Chain of Custody (Please complete all information)
Facility Q \(J L P O W llV )i Q f l : \A V1\ v fP rQ i t U
Ad.d. ress ^ Q ' r ^r?\iV. c. 7V7/aV ^ ^ ^
-J
ij O
1*
,, REED
Countv_ NPDES#.
Pipe/Outfall/Location , Instream Waste Cone
Sample'collected by (pnnt&sigjrl v lJ c ^ O n
Type of samp!e_j^l_(Grab):
Date. 11/ A ^
Affiliation PPU Vd/W. En C \. U>lo
Time 7 :0 0 '^>Ti ^
.(Composite): From Date.
Time.
To Date
Time
Subsamples comprising composite:
Number___________ Frequency of collection_________ Volume.
"Temperature of sample in sample collection device O CV
Final temperature of effluent at sample collection point o l \ 3 (7s
`fs sample collection device chilled?
Is sample packed on ice for shipment? bj&S
"It is required that all samples remain at 0 - 4C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency (Do not freeze!)
Is the sample chlorinated? ^
dechlorinated?
If so, how ?
__________ .
Permit with interim chlorine limit? ~ If yes - limit (mg/L)______ _____________
Field pH
. Field Total Residual Chlorine___________
Comments/Sample description (7? U -\e c S ]___________
ij\A (9r (VQtV\ RQ->/-i(Tj2.. J
t
Type of test(s) to be performed MtjeriC? Q h f trri p . C f"tjp fl nC?C^Ofl_____
(Specify organisms)
Q/O^kj u S __________________________
Method of shipment. p e u v e r ^ bvj f?CU
Print Sc Sign Names
1. Relinauished bv P r . \C y\ - UAa JL Date v / S / Q T ? Time tO ' - Q O a x
Received bv
Date U l 3 / 1 e) Time ld y<?
n Relinquished bv
Date H f 3 /*7 Time I O O
Received bv <
Date W3 Ie?") Time U 3 o
X k /-
^ 7-' H c a X ^ C J ^ ___________
( L i n i __________ /J -? o
JRAir `7 7 - 7 2 3 7 ?
FOR REED LAB USE ONLY Arrival Temperature
Color
Odor
DO (mg/L)_P H _________________
Conductivity (umhos/cm).
Salinity (ppt).
TRC (mg/L). _____ Method_________
On ice?
\---------------N
Solids
D-77
5 ;1 5 /9 4
US00001258
COASTAL BIOANALYSTS INC. EFFLUENT/DILUTION WATER DATA (FormE F D l.u i )
INITLAL (A R R IV A L) E F F L U E N T C H A R A C T E R IS T IC S :
Sample Date
11
Temp. (C) 1 3 . 3
1
Conduct. (uMHOS) 1 1V3G, 1
1I 1 -I
Salinity (g/kg) | /
|
|
|
-
1 I 1 |
------------- --------
!! 11 11 j
pH 1? .
i 11
Diss. Oxygen (mg/1) l u . a. 1
i i 1i
Tot. Chlorine (mg/1) l `-><_
11 11 1
NH3-N (mg/1) i , r i i i i 1 1
Hard (mg/1 CaC03) i o ; i
ii
i1
1
Alkln (mg/1 CaC03) a?-?> i i i i 1
1
Color/Appearance1 C Y
1 ,1
1
11
1
Initials
(fit 1
11
1
1
1
1 C-Cleai O-Opaque Y-Yellow B-Brown Bl-Black G-Green T-Toibid SI -Solids (S-Slight M-Moderate V-Very Much)
EFFLUENT ADJUSTMENT/POST-ADJUSTMENT MEASUREMENTS:
Date Temp (C).
I |
Salinity (g/kg) |
D O W !) Aeration Rate
1 1
Aeration Time (min) |
Adjust. D O. (mg/1) |
PH | Tot Chlorine (mg/1) |
Initials
l
I | | 1 1 ( |
| j
il
! | | I | | j
| [
t
| | |
| | j |
! j
1 1 1 1 i i i i 1 1
DILUTION WATER CHARACTERISTICS:
Date 1 1
|
Temp. (C) |
|
|
DMW:
| |
Conduct. (uMHOS) 1
|
|
|
Salinity (g/kg) |
|
||
PH 1 1 | |
Diss Oxygen (mg/1) |
|
|
|
Hard (mg/1 CaC03) |
1!
Alkln (mg/1 CaC03) I
|
|
|
Initials
till
H W M tv/
1' 1 1 1 1 1 1 1 1
f 1 1 1 i i i i 1 1
1 1 1 1 1 1 1 1
! I 1 1 i i i i 1 1
1 I 1 1 1 1 1 ! 1
ACUTE
I1I//3A? II.U HL/. iis-.o il -
II -
HUS. O IIN O If C^/i-
ACUTE
\\Q cS
1h o
1*0
II7 -.U II O r f ll/V-A II \t(Y
hm
E F F L U E N T ID: t^ s t - K c ^ ^ q , / '
A i T {,8
T EST ID: J n &
D. 78
US00001259
Coastal Bioanalysts Inc.
ODU/Oceana Acute Effluent Toxicity Tests
M ysidopsis bahia & CypriJiodon variegatus
Sample I.D. B3 Contract No. N00187-93-D-9748 * Delivery Order No. 0235 J.O. No. 1912290, Item No. 0002BJ, 0002BH
PWCNo. 1361 11/2/97 2015 Sample No. JRA97-12378
Submitted to: Ms. Merrill Anderson-Ashcraft
Navy Public Works Center Code 930, Building Z-140
Norfolk, VA 23511
2
By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062
(804) 642-0168
D-79
US00001260
ODI.:. OCEANA Sample I D. B3 11.197 Sam ple97-I237S Test ID: JRA97136
Coastal Bioanalysts Inc.
I. TEST RESULTS
Table 1. Summary Acute Test Results
% Effluent Concentration
0 63 12.5 25.0 50.0 100
Mean (%) Organism Survival
' M. bahia
24=h
48-h
100 100
100 100
95 95
100 100
95 35
60 5
C variegatus
24zh 95
48-h 95
100 100
100 100
100 100
100 100 100 100
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
45.0% 37.9-53.3 Trimmed Spearman-Karber
2.22
> 100% N/A N/A < 1.00
Acute Test Information:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
M. bahia Test 48-h Static Survival 11/3/97 1725 11/5/97 1740 CBI stock cultures 11/2/97 20 g/kg 20.0-20.6C No N/A
C variegatus Test
48-h Static Survival 11/3/97 1735 11/5/97 1730 Aquatic Bio Systems, Inc.
10/30/97 20 g/kg 20.0-20.6C
No N/A
Aeration necessary when effluent oxygen demand results in dissolved oxygen concentrations < 4.0 mg/1.
o
D-80
US00001261
ODIVOCE.VNA Simple I D. B3 1 1/2.97 Sample 97-I237X Test ID: JRA9713S
Sample Collection Date/Time
11/2/97 2015
Sample Use Dates/Times
11/3/97 1725/17353
Aeration2 Rate/Time
n/a
Pretreatment/ Adjustment
HWM Salts
2Aeration often necessary to reduce sample oxygen concentration to 100% saturation after warming. 3.U bahia/C. variegatus test time.
9
Table 2. Effluent and Dilution Water 4 Chemical & Physical Characteristics (Mean Value (Standard Deviation))
o
Parameter
Temperature (C)
Salinity (g/kg)
Conductivity (uMHOS/cm)
pH
Dissolved Oxygen (mg/1)
Total Hardness (mg/1 as CaCOj)
Alkalinity (mg/1 as CaCO-j)
Total Chlorine (mg/1)
n h 3-n (mg/1)
Initial Effluent
3.1 (...)
2 (-- )
1749 (-- )
8.14 (- )
11.8 (- )
132 (- )
325 (- )
<D.L. (-- )
13.5 (- )
Adjusted Effluent
20.7 (- )
21 (-- )
N/A
Dilution W ater
20.7 (...)
20 (...)
N/A
7.87 (- )
8.0 (- )
N/A *
N/A
7.85 (-- ) 7.9 <" > N/A
N/A
N.D.
N.D. .
N.D.
N.D. -
4Efnucnt parameters measured upon receipt of sample in lab and after adjustment on day of use. Dilution water (Wimex hw-Marincmix in ASTM Type I deionized water) values measured after preparation of diluent on day of use.
D-81
US00001262
O D I-O C E A N A S am pli I.D. B3 l | . r 9 7 Sample 97-12378 Tesi ID : JRA97136
H. WATER QUALITY
Coastal ioanalysts Inc.
D-82
US00001263
' n c
Dfeflig Tst"^'W
2712/97 >100 2728/97 Invalid 3/12/97 >100 3/26/97 >100 9/30/97 65 11/3/97 45.0
CUMULATIVE DATA SUMMARY NPDES Permit #: N/A
Oceana - ODU
B-3
-Vertebrates.^^'-.---V--.'-CInvertebratY fftO i LC50-%` " ^ ' ' >100 >100 >100 >100 >100 >100
!
Supplement
D-83
US00001264
ODI O C K AN'A Sample I.D. B3 1 i , 1'97 Sample 97-1237S Test ID: JRA97I36
UI. OUTFALL DATA SUMMARY
Coastal Bioanalysts Inc.
Test Date
9/30/97 11/3/97*
Table 4. Cumulative D ata Sum m ary - Acute & C hronic Tests (Tests conducted on this outfall by CBI)
Invertebrate
Acute Tests
Chronic Tests
1X50 T.U.
NOEC U L
65.0% 45.0%
1.54 * 2.22
N/A N/A N/A N/A
V erteb rate
^
Acute Tests LC50 U L .
Chronic Tests NOEC T.U.
>100%. <1.00 >100%. <1.00
N/A N/A N/A N/A
Results submitted with this report
IV . Q U A L IT Y A S S U R A N C E
M. bahia - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
C. variegatus - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (9.5% C.L.):
CBI Stock Cultures 10/15/97-10/17/97 KC1 (Sigma "Ultra" Lot#76H 10391) 343, 490, 700, 1000 mg/1
100% v
574.2 mg/1 (540.9-609.5) 612.1 mg/1 (488.3-735.9)
g
Aquatic Bio Systems, Inc. (same batch as test) 11/3/97-11/5/97 KC1 (Sigma "Ultra" Lot#76H 10391) 432, 720, 1200, 2000 mg/1
100%
1200.0 mg/1 (1070.5-1345.2) 1074.9 mg/1 (891.2-1258.6)
i
D-84
US00001265
C O A ST A L B IO AN AL YST S. IN C . N P D E S S T A T IC A C U T E / S A L T W A T E R
(Form A M B 4#)
i (ffluanl
Oay 1 1 In
Day ?
9 Lit
Conilo! $.25
C-l 1 c
1o
LLC-2 ____
_________
t5
M It
_ L ___________
!2 U
U>
2-1
12.5
2-2
> b ________
IO
3-1 i l
_ L __________
25 3-2 1C
-1 f
_ 3 ___________
50 4-2
H
_ J _________
5-1 _____ __________
t
100 5-2
D
_ J __________
Obsarvar A H _________ O H
Tina:
< l - i _____ n - H
fin ali Survrval iO C
\ 0c
1 C
-*>5 5
Sorci: cat
Hatch Dateffmw:
Acclirmtion w a ta r
A u bAcclirmtion Tanp. (rang*):
.
to rlD - if\
/d Olhar
OFaadng during test Not fad
Artarraa \ X
l Othar:
O u t* .: 300rrtQ
SCO ni Q lO C O n iQ Oth;__________i
last ydum ; ^00 ^ a 2S0ffi
5 0 0 ai
O r t :____________ni
I n tu no. garaTM par ra tto :
I Q _______ ** ratd" : _ s 5 --------------
Ptattopanott 161:80 3 O rt :________ R in to n a c o lattatola no. `j ~ /rJ )
fSETUP: D ito I /
7 _ T irm W aurA ddad I J - c C i _________
( W T -T*m Annali Adrted
_Sot UpBy.
INSTRUMENTATION USED:
A ISiam T h in iM iiw R o t o :
YS3 0 .0 . Mota A 1 |S B 0 H t7 S ) CP D e t o n a pH M atar (09500563) Sanom o R160P B a l i (<0020093)
TS SCT 3 3 M at (H8016792)
[ 3 Y 3 0 .0 .M atant2 (M IA 2S329)
Q Cotoing 2 4 0 fti M utar ( 9 6 2 6 8 )
SPPortapKZ M atar (8S236) O J S p a rt ATC R o h to t o r a u r (A366)
Othar
Day 0
Dayl
Oay?
cC
D o .Z
<Dii 'C
")/?
E M
pn
0 .0 .
(mg'!)
2 3 4 5
c
1 2 3 4 5 C
1
2 3
5
sc A1 l
2
N3 IsM 4
5
Radicata 9
Initials:
7 /7 '
A c ' {.
A & ,S '
X n l.
A o7
A $' L-*
A i. V
A / ) 7-
< j <3 S ~ *
A y.-?
J /' 7
> .9 T
77<
Z 73
7- i f ? 7 - SA
77 7 -? 1
77 H
77
> .< ? > 7 - , 6 '? -
ft i \ 7
7 fV
H?
7 . ?
?.f A
* 7 :7
7- -1
7 -7
? ft" 7 (
7 - Li 77/
> 1
7 .7
7 ./
7 7 7 7 ~ 7 .a .__ 7- "? r___7 ,.JLfl-- ^ & ~ 6 _ 7 . 1 - ^ 3 A /; 7 D
A QA______ 1 A D
A f
AD
1;
1 ___
A 1)
A,
<30 Ai
A_ ))
7 ! - A ------7 a 4-
C-tf _ L i i _
NOTES:
Qualify Aasurwca:
As Qudity A u t n o ffc a r for th n test I cartify that no ynficanl d w u to m from x c a p ta d protocol decurtati during fOuiina im p a c tw (ta to u othorwira m u d bota) and that Ita to la accorataly rallact tlu t t a t It w aa partotmad by C8I.
Contrants
_
-- r!k - j b ( r s i ) t
U iih
TEST I . D . : _ 3 i l ^ 5 i l 3 k D-85
US00001266
trim m ed spea rm a n - k a r b e r m e t h o d ,
v er sio n 1 .5
DATE: 11/3/97
TOXICANT : B3 SPECIES: M BAHIA
TEST NUMBER: JRA97136
DURATION:
RAW DATA: ------------
Concentration ( %)
.00 6.25 12.50 25.00
50.00 100.00
SPEARMAN-KARBER TRIM:
Number Exposed
20 20 20 20 20 20
it
5.00%
Mortalities
0 0 1 0 13 19
SPEARMAN-KARBER ESTIMATES:
LC50:
95% LOWER CONFIDENCE:
95% UPPER CONFIDENCE:
44.96 37.91 53.31
48 H
nrnjiT T irv D p n p rR T T n N S WERE NOT MONOTONICALLY IN C R E A SIN G .
*i0TE: S S s t S nTS S ereTM ! 5 S S . - S BPEARMM.-KAEBERJSTIMATIOR.____________
D-86
US00001267
COAST.VI. moVN.\I.VSTS. INC NPDfcS STATIC ACt TKS.U.TW ATKR
ACV4X)
1 filli*
10.
Control
S 25 12.5 25 SO 100
C-I C-2 11 12 21 2-2 3-1 3-2 4-1 4-2 5! S-2 Observer 1 me:
Oay t # Live
Day 2
9 In
Final \
Survival
5 ____ it) 0 1 i >
Ifj
la :
M
10
\0 0
If it)
I
'6
w 10
i9
/;
i
to
It)
10
/
(O o I OO
\cO
o O
'(> i n
( r i i - .... G H m s ^3^
4
o-c.ps- C vonnodon varieqatus
Sauce:
C6I
Acclimation water. HWM ASW ^ 2
O ther
S aiinrr G /N
.
Arriiironon Temo, iranael:
0.
- 1> f n
Feednu cvw to test: A rtem a --J X
id Other.
1
Feed) Amng test: Not led I Artema
fd Other
Chamber s a e : 3 0 0 ml Q
500 fri Q
1000 fri ] O ther
230n<
2S0irf
500 tri { S O ther
tri fri
No. implicates: r - J
'
PSrfutwrod 161.80
OlW :
R a n d M ttJtx n t e n d a le no.
3
STUP'D. 11 ( 3 f 4 T Turn A n n u ls Addld 1
T inW jl*rM *d l ^ c ^ D
Sm Ud BJ P O ( - i
IN SIHUM tNIAllON USED:
AStem Therrom eter N vxrir
f
O YS D O. H m 1 ISNEB01 t h s )
m CP O d ie r n a pH M eter (095005631
Sw orn H160P Bohre H0020093)
r s SCT 33 M erer (H8016792)
C Q YS 0 .0 . M eur 9 2 (SW 1A02S329) L J C omn 2 4 0 fH M ater ( 9 6 2 6 8 )
SP PtrucK2 M W (8S2380)
Spwran ATC Refractom eter (A356)
Oay 0
Oay I .
Oay 2
T
E
M
P
c 1 2 3
4
5 C
pH
S c 1
0.0. (mt
2 3 4 5
SC
A1 l
2
N3
4
5 Replicate #
listiate:
2 0 .-7
<3 (ii ^
7 a q-
1 0,L e
n 3v V
K M <50 3
.e r y .n
T -.3 ?
-f
7 .f2
T -.itf
M
T -f T -.f
7-.1
hi
f.to o -.u ? 3-T L f T -.S U M sS ', t o / S' 7- ? ? /r 7 7?
T -.lo
- i t l/
J. .-T
l o So
VG f .U S '
+ .u S
7 77 7 .1 6
*.e 3
7V 77 7 .0
b .V
Jb
<^7 <3/ 3/
A C rff
.. 4 t l
a*
LU _ 3 (i =J c
0 G i _______
2 / J . _______
3 / ________
<D M ________
____
NOTES:
-
Quality Assurante: As Qualiry A i u r w c t or licer lor th n test l certify that no uqrficartt O vations (rum accepted pmtiwol a l u n n i ilunng mutine insp-lw * (wriess otherwise noted betowj and that the eU cu rM d y ^**c*th* test as it was portoni d CB1.
n
) - - - -------------- S.9MIW
r
ith(9~>
0t
T E S T I D 2x B j S ..S . 2 - L K
D-87
US00001268
COASTAL BIOANALYSTS INC. EFFLUENT/DILUTION W A T E R D A T A (Form EFDL0I95)
INITIAL (ARRIVAL) EFFLUENT CHARACTERISTICS:
Sample Date I | | / ; ) / ^ .? I I 1 - 1 1 1
Temp. (C) | ^ , i I I I I I I
Conduct. (uMHOS) |
i I I I I
Salinity (g/kg) | 0
l
I
I
t
I
I
pH I S . I H
II II I
Diss. Oxygen (mg/l) | ( / , ^ I I I I I I
Tot. Chlorine (mg/l) | I I I I I I
NH3-N (mg/l) | / 3 . I
I
i
I
I
I
Hard (mg/l CaC03) | / ^ Q I I I I I .... I
Alkln (mg/l CaC03) | \ O S ' I I I !
...J_------------- !-------------
Color/Appearance1 | L /
1 ,,1
1________ !_________ !--------------!-------------
Initials
| (r(T
1
1_________ !________ 1 ____ !--------------!-------------
4 C-Clear O-Opaque Y-Yellow B-Brown BI-Black G-Green T-Tuibid SI -Solids (S-Slight M-Moderate V-Very Much)
FFFLUENT ADJUSTMENT/POST-ADJUSTMENT MEASUREMENTS:
ACUTE
Date | 1 I I 1 1 1
Temp. (C) |
i
111
1 1 II3&.?-
Salinity (g/kg) | 1 1 1 1 1 1 M l
D O (mg/l) f i l l
1 I lg .G ...
Aeration Rate | 1 1 1
1 II -
Aeration Time (min) | 1 1 1 1 1 1 II--
Adjust. D O. (mg/l) |
|
1 1 1 1 1 II-
pH 1 1 1 1 1 1 1
Tot Chlorine (mg^l) | 1 1 1 1 1 1
___
Initials
|
|
1 1 1 1 1 II C'H
DILUTION WATER CHA RACTERISTICS:
DMW:
Date |
|
II
Temp. (C) |
|
I1
Conduct. (uMHO?) |
|
II
Salinity (g/kg) l i l t
pH |
Diss Oxygen (mgl) f
11
ill
1
Hard (mg/l CaCO) | 1 1 1
Alkln (mg/l CaCO) | l I I
Initials |
1
HWM:V /
1 1 1
1 1 1
11 1
1 1 1
1 1 1 1 1 1
1
ACUTE
Illin W AoT
-----
II 3.0 . -S-
II?-. f
llfvfl____
____ 110/f-
EFFLUENT \D \ l L__
J/ i u l
TEST ID T n 4 D-88
US00001269
Toxicity Test Sample Chain of Custody (Please complete all information)
Facility Q l c P Q V rtiv O iQ n U n n f ^ r Q i t U
Address
. VAr ,4?^ P^jf______
|3 < W
REED.
4?'
s P c i.^ V
County
Pipe/Outfall/Location
NPDES*.
Instream Waste Cone
Sample'collected by (print&sigpj J c ^ O H
Affiliation DDU
EnL/)U
Type of sample ^ (Grab):
Date. " / <*-/c\~7 Time % j'5~ pY l
.(Composite): From Date.
Time.
^
To Date__
Time
Subsamples comprising composite:
Number________ Frequency of collection_________ Volume.
'Temperature of sample in sample collection device 0 (L>
Final temperature of effluent at sample collection point rO \ X > Qj
'Is sample collection device chilled? C-S Is sample packed on ice for shipment? vjg-S
'It is required that all samples remain at 0 - 4C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency (Do not freeze!)
Is the sample chlorinated?
dechlorinated?
If so, h o w ? _ V f* -___________
Permit with interim chlorine limit? ~ If yes - limit (mg/L)______ ~ ____________
Field pH 7. T O
Field Total Residual Chlorine___________
Comments/Sample description
- cl LdC f?
0j Q*\1y%LAA<c\
__0_)_0_ PpppYrvY]i___A__F_P__F_____q_y__j tA_____________________________________
Type ortest(s) to be performed
^.Fnrtl p - C r u p f t idOciofl
(Specify organisms)
\ ! T \ Q sQ \\\A . ^
Method of shipment. p e W e rij bej C U
Print & Sign Names
1. Relinquished bv P r . E r \C y\ ~
Date jv/ S / A 7 t TM , tQ ' - C C a r t
Received bv VJ P Relinquished bv U Ij,,
Date 1l J j P Date 1HOl <f ?
Time / O V-i> Time / / 3 ^
Received bv ---- & /
vo-Ccx_^ot-^-_
Date " h 9 i Time 113 O
__________
n i z f r i ___
/JO G
FOR REED LAB USE ONLY
JRA# Q ' I ' l Arrival Temperature
Color
Odor
pH_________ DO (mg/L)_______ Conductivity (umhos/cm)_
Salinity (ppt)_______ TRC (mgT)______ Method________
On ice7 Solids
1
D-89
5/1/?*
US00001270
L)c*Xj.
L
n~Z-ep
j
l-i-fl
__
l^ 3 -
/n= ,
i s
D-90
US00001271
CHAIN-OF-CUSTODY RECORD
CLIENT IN F O R M A T IO N
m r& >
E N V IR O N M E N T A L
& ANALYSIS REQUEST FORM'
PWC ENVIRONMENTAL LABORATORY CODE 9 3 0 BLDG Z-140 9 7 4 2 MARYLAND AVENUE NORFOLK, VA 2 3 5 1 1 - 3 0 9 5 ` PH: (7571445-8851 FAX: (757/445-8852
C O M P A N Y/C O M M A N D : ( ' Y . p ^ a -
CODE:
CONTACT.
f i X _____R : j L _________ ___ "" \
PHONE:T fcY -S 'l-.S n EXT:
FAX:
J O I T / A 3-(l c7
S IG N A TU R E : c.-v
P E R M IT NO.:
LAB. USI: ONLY LINE ITEM SAMPLE NO SAMPLE IO/LOCATION
OOo'A H
'-p'T. 1 ' - / A Y / <p
- S iB O D AB T 1 4
F * * A 7'uc. I<L_
,3 % 1& I* 1
1 '/-/A V ? Y
B fiFcr-r
Bol IUTi't IbftAii/r 'I7-/A 3S
13 /i 3> 1 AFT/' i t V W r . ^ l1-IP-S%(
13, ( 4 yY 3> - CoaV d (
a ~ 'w a t f a - L 121* & jpx Ps-- CoAro[
UM/U OH Al TlPE SAMPLED
UMn DATE IHE
6l MAlflII
SIAR1
STOP ll:iD & STARI
7
Slop STARI
O'l oo
-4 -
STOP START I
M /<f
STOP 1 START i
oS'co
STOP 1 START \
c'/i'T
STOP i STARI 1
SfJlL
siop ;
-
UOPSAMPLES PRESERVATIVE /CONTAINERS
ANALYSIS
Fit LD READINGS PRESERVAI!. P thperaiure OlHCR VERiflEUEi
/ --- i(---p---;------ 4......... o F ' i 7 : r f v - ' f.... ip.hH J?A ^
4
7- s o 1 - *' : 3-- ----------------
`m ~ 1 {.
7. y J?0.J
..S<W3'h ^ 7 S 7 Z ) . c>
G -GRAB
CF-COMPOSITE, FLOW CT- COMPOSITE, TIME
L- LIQUID SW- SWIPE S- SOLID GS-GAS
SS- SEMI SOLID
P PLASTIC GL- GLASS T-TEFLON V *VOA
BBAG
A- AMBER(glass)
C-CARTRIDGE
TL-TEFLON LINEDLID
H-HEXANE RINSED
1 COOLTO4C 2-HN03, pH<2 3 H2S04, pH<2 A- NaOH, pH>12
6-HCL. pH<2 1 6- 0.006%Na2S203, 4C 7- FIELDFILTER 8- NONE
T U IH A IO U M D (D /rD :
COMMENTS:
(FOR RUSH TURNAROUND STATE REASON BELOW)
R E G U LA TIO N APPLIED:
R C R A ( )..
HRSD ( )
SDWA ( )
TSCA ( )
CWA ( ) PHOTO ( )
CAA ( ) OTHER ( )
SAMPLING/COLLECTION CHARGE: POSSIBLE SAMPLE HAZARDS: COMMENTS:
SAMPLE DISPOSAL: ( )RETURN TO CLIENT ( )DISPOSAL BY LAB
LV. NUMBER:--
CONTRACT LAB:"
IN IT.:
DATE:'
I. C U S T O M E R i s R E S P O N S I B L E F O R ALL C H A R G E S N E C E S S A R Y F O R T H E P R O C E S S IN G A N D A N A L Y S I S O F S A M P L E IS I
ALLRUSH SAMPLESARE SUBJECTTOSURCHARGE
CONTRACT NO.(S):
'OFFICIAL-USEONLY
_2^ ^ ^ M P L E ^ R ^ E IV E D ^ A F T E R ^ 2jO O ^ P M ^ M O N ^ JN U R S ^ A N D 1:00 PM ON FRID A Y WILL B E P R O C E S S E D TH E N E X T B U S IN E S S DA Y ( 7 : 0 0 A M 3 : 3 0 PM )
FOR LAB USE ONLYII
REJECTED ( )
REASON:
HOLDING TIME( )
SAMPLES VERIFICATION
CONTAINERS! )
IMPROPERLY SAMPLED! ) OTHER! )
"
TIATEil IME
D-92
US00001273
Coastal Bioanalysts Inc.
ODU/Oceana A cute Effluent Toxicity Tests
M ysidopss baha & Cyprinodon variegatus
Sample I D. B2 Contract No. N00187-93-D-9748 * Delivery Order No. 0235 J O. No. 1912290, Item No. 0002BJ, 0002BH
PWC No. 1362 11/2/97 2000 Sample No. JRA97-12379
Submitted to: Ms. Merrill Anderson-Ashcraft
Navy Public Works Center Code 930, Building Z-140
Norfolk, VA 23511
9
By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062
(804) 642-0168
D-93
US00001274
O D I'.O C E A N A Sample I D. B2 11/2:97 Sample 97-12379 Test ID: JRA97135
Coastal Bioanalysts Inc.
I. TEST RESULTS
% Effluent Concentration
0
6.3
12.5 25 0 500 100
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acute Test Results
1 Mean (%) Organism Survival
M. baha
C. variegalus
24-h 4M
2M 4M
IC) 100 100 100 100 100 100 100 1 0 0 50 65 0
100 100 100 100 100 100 100 100 100 100 100 100
50.0% 42.8-58.4 Spearman-Karber
2.00
> 100%
N/A N/A
< 1.00
Acute Test Information:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
M. bahia Test
48-h Static Survival 11/3/97 1720 11/5/97 1730 CBI stock cultures 11/1/97 20 g/kg 20.0-20.6C No N/A
C variegatus Test
48-h Static Survival 11/3/97 1730 11/5/97 1720 Aquatic Bio Systems, Inc.
10/30/97 20 g/kg 20.0-20.6C No N/A _
'Aeration necessary when effluent oxygen demand results in dissolved oxygen concentrations < 4.0 mg/1.
1
D-94
US00001275
O D I'.OCEANA Sample I.D. B2 11,2/97 Sample 97-12379 Tesi ID: JRA97135
Coastal Bioanalysts Inc.
Sample Collection Date/Time
11/2/97
2000
Sample Use Dates/Times
11/3/97 1720/173 03
Aeration2 Rate/Time
n/a
Pretreatment/
Adjustment
HW M Salts
2Aeration often necessary to reduce sample oxygen concentration to 100% saturation after warming. 3\,/ bahia'C. variegatus test time.
Table 2. Effluent and Dilution Water 4 Chemical & Physical Characteristics (Mean Value (Standard Deviation))
Parameter
Temperature (C)
Salinity' (g/kg)
Conductivity (uMHOS/cm)
pH
Dissolved Oxygen (mg/1)
Total Hardness (mg/1 as CaC03)
Alkalinity (mg/1 as CaCO' )
Total Chlorine (mg/1)
n h 3-n (mg/1)
Initial Effluent
2.7 (--)
2 (--)
'1775 (-)
8.18 (-)
11.8 (-)
140 (-)
375 (--)
<D.L. (-)
14.0 (-)
Adjusted Effluent 20.5 (--) 20 (...) N/A
7.85 (~ ) 8.0 (-> N /A .
N/A
N.D.
N.D.
Dilution W ater 20.8
(...)
20 (-) N/A
7.76 (_-) 7.9 (-) N/A
N/A
N.D.
N.D.
4Effluent parameters measured upon receipt of sample in lab and after adjustment on day of use. Dilution water (Wim hw-Marinemix in ASTM Type I deionized water) values measured after preparation of diluent on day ot use.
D -9 5
US00001276
ODI*/OCEANA Sample I.D. B2 11.2.97 Sample 97-12379 Test ID: JRA97135
II. WATER QUALITY
Coastal Bioanalysts Inc
Parameter Temperature
(C) DO. (mg/1) pH
Salini tv <g/kg)
Parameter Temperature
(C) DO. (mg/l) PH
Salinity. (gAg)
Control
20.5 (0.3)
7.9 (0.1)
7.75 (0.02)
20 (0)
Table 3 Water Quality Summary
M . baha Test
Mean Value (Standard Deviation)
6.3%
20.4 (0.2)
12.5%
20.4 (0.2)
25%
20.4 (0.2)
50%
20.3 (0.2)
7.8 (0.2)
7.81 (0.06)
20 (0)
7.7 (0.3)
7.86 (0.02)
20 (0)
7.7 ' (0.3)
7.93 (0.04)
20 ()
7.6 (0.5)
8.00 (0.13)
20 (0)
1Q0%
20.4 . (0.2)
7.6 (05)
8.05 (0.19)
20 (0)
Control
20.5 (0.3)
7.8 (0.2)
7.72 (0.04)
20 (0)
C. variegalus Test
Mean Value (Standard Deviation)
6 3%
12.?%
25%
50%
20.4 (0.3)
7.7 (0.2)
20.4 (0.3)
7.7 (0.2)
20.4 (0.3) E
7.6 (0.3)
20.3 (0.3)
7.7 (0.4)
7.76 (0.08)
7.84 (0.05)
7.92 (0.03)
7.97 (0.11)
20 20 20 20 (0) (0) (0) (0)
100%
20.3 (0.3)
7.6 (0.5)
7.98 (0.13)
20 (0)
D-96
US00001277
O Dl'OC'E.V<A Sample I D. B2 11.1 9 7 Sample 97-1239 Test ID: JR.V27135
III. OUTFALL DATA SUMMARY
Tpst Date
9/30/97 11/3/97*
Table 4. Cumulative Data Summary - Acute & Chronic Tests (Tests conducted on this outfall by CBI)
Invertebrate
Acute Tests
Chronic Tests
LC50 TAL
NOFC T.U.
66.0% 50.0%
1.52 2.00
N/A N/A N/A N/A
Vertebrate
Acute Tests
Chronic Tests
LC50 T.U.
NOEC UL.
>100% <1.00 >100% <1.00
N/A N/A N/A N/A
" Results submined with this repon
IV. QUALITY ASSURANCE
M. bahia - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
C. variegatus - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: ' LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
CBI Stock Cultures 10/15/97-10/17/97 KC1 (Sigma "Ultra" Lot#76H10391) 343, 490, 700, 1000 mg/1 100% 574.2 mg/1 (540.9-609.5) 612.1 mg/1 (488.3-735.9)
Aquatic Bio Systems, Inc. (same batch as test) 11/3/97-11/5/97 KC1 (Sigma "Ultra" Lot#76H10391) 432, 720, 1200, 2000 mg/1 100%
1200.0 mg/1 (1 0 7 0 .5 -1 3 4 5 .2 )
1074.9 mg/1 (891.2-1258.6)
D -9 7
US00001278
CUMULATIVE DATA SUMMARY NPDES Permit #: N /A Oceana - ODU B-2
D a te ^o f^ Invertfaraf^^^/i.:';' jVeitebratek'Sft-A'.
jfnvextebratc-;:^-'^ i.. VertebratV:; .. j*
Test" - ''.'5
EC50-.%:
- - ;..S N E C % 'r -
n 6 e c % '` 't ` "
2/12/97 >100
>100
2/28/97 Invalid
>1Q0
3/12/97 >100
>100
3/20/97 >100
>100
i
.
3/26/97 >100
>100
9/30/97 66
>100
11/3/97 50.0
>100
Supplement
D-98
1 US00001279
COASTAL BJOANALYSTS. INC. NPDES STATIC ACUTEVSALTWATER
(Form AMB4S)
1 E llluenl
C ontrol
6 .2 5 1 2 .5 25 50 100
Oay 1 I.D.
f iiv*
Day 2
9 Lrva
Final \
SuvrvW
Cl l 0
ro
lO o
U lC -2
(E
_______
___________
M '6
12 0
l
iC fc >
2-1 u >
/6
7 - 2 u > ... _ _____________ ^
3-1 If/ r o
3 - 2 _______ _______________ - J L k ______________ 4-1 1G
4-2 ______ ________________
5-1
(z
.
-
5-2 0
______________
G> H
C ri
i 0c( O
5o 0
Tim a:
>To<
M v s id c p s is b a h 13
1Oaia/Trm: ( \ f U * \ -
CBI
AcchfTB iion w a l i r HW M ASW
O l h a r ________________Salinity , 3 |* \
AcchrrotiDn Tamp (range): r - O o - Q ____________ < Q o > A n _____________
F a* n g poor to test:
A n i m a ^ )(
/ d 0U w .__
F vetkr^ duing m l : Not fa d
\Xk n m *
/d
Othar._
C h a n te r M i*:
^a
5 0 0 irf O
1 0 0 0 iri Q
O th ar
T t lu m : 200 H
S O n iQ
SCOni Q
O th * r_
In tia l no. o rg w tn m p *r f i r f i o t K
/ ^ _______ Na. W ft o W
QOw:F t a t o p a n o d 1 6 1 :8 0 3
fladyrm w i t a m p b t a n o . 3 - - *
1 1 8 WS E T U P : 0 t *
-- Tim W a ta r A c t e d i & O i ____________
T u n A m ra ls Adcfrd_
__________ s * U p B y
INSTRUM ENTATION USED:
S lam T h an ro m atar f itf r t T A I
T S 0 .0 . M at*r 1 1 ( S B 0 1 4 4 7 5 ) I C P D *js*** jH M atar (0 9 5 0 0 5 6 3 )
IS a r r o n u t R 1 6 0 P B a la n a ( 4 0 0 2 0 0 9 3 )
Y S SCT 3 3 M *t*r (H 8 0 1 6 7 9 2 )
SY S 0 .0 . M alar 9 2 { 9 tA 0 2 6 3 2 9 ) C o m in g 7 4 0 jH M a l a r ( S 7 C 2 6 8 )
D SP PonacH 2 M atar (85 2 3 6 0 )
j S p ^ ta n ATC ftafractw m w r (A 366)
D *y0
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Oay 2
C
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3
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2 3 4 5 C 1 2 3 4
5 R a d ica i* 9
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0o.:) l\v J
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2Q
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^b
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... ' D o
2J x l ________
> C r ii-
O
Oh
_ __________ C -rt
NOTES
Q uality A s t f w n :
A s Q uality A t w a r t o M icar lo r th*s ta ti 1 c e rtify th a t r o tig m lie an l d o n a tio n s Irem a c c a p ia d pro to co l o c cu rre d dunng reatin a r a p a : ito r t (u n i ,! o lh a rw tsa rv ta d b a to n ) *>d th a t th* c a ia accuretaly rvllact tha la s t K W M parform ad by C 8I.
Colmanti
Lyxiu
i/ t ^ '7 _
TEST I D : y t - f l 4 I f t S
D-99
US00001280
TRIMMED SPEARMAN-KARBER METHOD.. VERSION
DATE: 11/3/97 TOXICANT : B2 SPECTES: M BAHIA
TEST NUMBER:
RAW d a t a : -- --
Concentration
<*>
.00
6.2S
12.SO
25.00
SO.00
100.00
.
SPEARMAN-KARBER TRIM:
Number Exposed
20 20 20 20 20 < 20
.00
SPEARMAN-KARBER ESTIMATES :
1X50:
95% LOWER CONFIDENCE:
95% UPPER CONFIDENCE:
DURATION:
Mortalities
0 0 0 100
20
50.00 42.82 S 8 .38
CciAST.U. UI<;.VN.U.YSTS. INC. NPOCS STATIC A C U T E /S A LnV A TE R
(Fvrm A C V JK)
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TEST I D ) f l& < 1 1 ^
D-100
> US00001281
Coastal Bioanalysts Inc.
ODU/Oceana Acute Effluent Toxicity Tests
M ysidopsis bahia & Cyprinodon variegatus Sample I.D. B1
Contract No. N00187-93-D-9748 * Delivery Order No. 0235
J.O. No. 1912290, Item No. 0002BJ, 0002BH PWCNo. 1363
11/2/97 1915 Sample No. JRA97-12380
Submitted to:
Ms. Merrill Anderson-Ashcraft Navy Public Works Center Code 930, Building Z-140 Norfolk, V A 23511
By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, V A 23062
. (804)642-0168
D-101
US00001282
<;D(' 0CE.VNA Sample I.D. B! ll-'2.*97 Sample 97-12380 Test IDr JR.-\97I34
Coastal Bioanalysts Inc.
I. TEST RESULTS
% Effluent Concentration
0 6.3 12.5 25.0 50.0 100
48-h LC50. 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acute Test Results
Mean (%) Organism Survival
M - bahia
2h
48-h
100 100
100 100
100 100
100 90
100 65
80 0
C. variegatus
2 i h 48-h
100 100
100 100
! 100
100
100 100
100 100
100 100
50.5% 41.7-61.2
Probit 1.98
> 100% N/A N/A < 1.00
Acute Test Information:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?*: Aeration Initiation Date/Time:
M. bahia Test 48-h Static Survival 11/3/97 1715 11/5/97 1705 CBI stock cultures 11/2/97 20 g/kg 20.0-20.6C No N/A
C variegatus Test 48-h Static Survival 11/3/97 1701 11/5/97 1715 Aquatic Bio Systems, Inc. 10/30/97 20 g/kg 20.0-20.6C
No N/A
'Aeration necessary when effluent oxygen demand results in dissolved oxygen concentrations < 4.0 mg/1.
N
D-102
V US00001283
O D l'O C n.V N A Sample I.D. BI II 1 9 ? Sam ple97-123X0 Tesi ID: JR.A97I3-I
Sample Collection Date/Time
11/2/97 1915
Sample Use
Dates/Times
11/3/97 1715/17013
Aeration2
Rate/Time
n/a
Pretreatment/ Adjustment
HW M Salts
d e ra tio n often necessary to reduce sample oxygen concentration to 100% saturation after wanning. 3;W. b ah ia C. variegatus test time.
Table 2. Effluent and Dilution Water 4 Chemical & Physical Characteristics (Mean Value (Standard Deviation))
Parameter
'
Initial Effluent
Temperature (C)
3.7 ' (-)
Salinity (g/kg)
1
(...)
Conductivity (uMHOS/cm)
1671 (-)
PH 8.17 (-)
Dissolved Oxygen (mg/1)
11.8 (-)
Total Hardness (mg/1 as CaCO-j)
130 (-)
Alkalinity (mg/1 as CaCOj)
350 (-)
Total Chlorine (mg/1)
<D.L. (-)
n h 3-n (mg/1)
8.6 (-)
Adjusted Effluent 20.9
(...)
20 (-- ) N/A
7.85 (--) 8.0 <-- ) N/A *
N/A
N.D.
N.D.
Dilution W ater 20.8 (-) 20
(...)
N/A
7.76 (-- ) 7.9 (-) N/A
N/A
N.D.
N.D.
4Efllucnt parameters measured upon receipt of sample in lab and after adjustment on day o f use. Dilution water (Wimex hw-Marincmix in ASTM Type I deionized water) values measured after preparation of diluent on day of use.
D-103
US00001284
(JD I - O C E A N A S im p le I.D . B I 1 1 .^9 7 Sim ple 97-12380 Test ID: J R .W 1 3 4
II. WATER QUALITY
Coastal Bioanalysts Inc.
Parameter Temperature
(C) DO. (mg/1) pH
Salinity (g/kg)
Parameter Temperature
(C) DO. (mg/1) pH
Salinity (g/kg)
Control
20.4 (0.4)
7.8 (0.2)
7.73 (0.02)
20 (0)
Table 3 Water Quality Summary
M . bahia Test
Mean Value (Standard Deviation)
12.5%
25%
50%
20.4 (0.4)
20.4 (0.4)
20.4 (0.3)
20.4 (0.4)
7.7 (0.2)
7.79 (0.07)
20 (0)
7.6 (0.3)
7.83 (0.01)
20 (0)
7.6 (0.5)
7.87 (0.04)
20 (0)
7.5 ' (0.4)
8.00 (0.13)
20 (0)
100%
20.4 (0.5)
7.4 (0.7)
8.03 (0 17)
20 (0)
Control
20.3 (0.5)
7.8 (0.2)
7.73 (0.03)
20 (0)
C. variegatus Test
Mean Value (Standard Deviation)
Lm
20.3 (0.4)
7.6 (0.3)
12.5%
20.3 (0.5)
7.5 (0.4)
25%
20.2 (0.5) je 7.4 (0.6)
50%
20.2 (0.5)
' 7.4 (0.6)
7.79 (0.09)
7.82 (0.02)
7.89 (0.02)
7.94 (0.08)
20 20 20 20 (0) (0) (0) (0)
100%
20.2 (0.6)
7.4 (0.8)
8.00 (0.13)
20 (0)
D-104
US00001285
O D IO C E .\ N A Sample !.D. B I 11. 2:97 Sample 07-123X0 Test ID: JRA07134
DI. OUTFALL DATA SUMMARY
Coastal ioanalysts Ine.
Test Date
9/30/97 11/3/97*
Table 4. Cumulative Data Summary - Acute & Chronic Tests (Tests conducted on this outfall by CB1)
Invertebrate
Acute Tests
Chronic Tests
LC50 T.IJ.
NOEC U L .
72.0% 50.5%
1.39 1.98
N/A N/A N/A N/A
Vertebrate
Acute Tests
Chronic Tests
LC50 T.IJ.
MfEC T.U.
>100% <1.00 >100% <1.00
N/A N/A N/A N/A
` Results submitted.with this report
JY. Q U A L IT Y ASSURANCE
M. bahia - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
C variegatus - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
CBI Stock Cultures 10/15/97-10/17/97 KC1 (Sigma "Ultra" Lot#76H10391) 343, 490, 700, 1000 mg/1 100% 574.2 mg/1 (540.9-609.5) 612.1 mg/1 (488.3-73.9)
Aquatic Bio Systems, Inc. (same batch as test) 11/3/97-11/5/97 KC1 (Sigma "Ultra" Lot#76H10391) 432, 720, 1200, 2000 mg/1 100% 1200.0 mg/1 (1070.5-1345.2) 1074.9 mg/1 (891.2-1258.6)
D-105
US00001286
EPA PROBIT ANALYSIS PROGRAM USED FOR CALCULATING LC/EC VALUES
Version 1.5
JRA97134
Cone.
Number Exposed
6.2500 12.5000 25.0000 50.0000 100.0000
20 20 20 20 20
Number Resp.
0 0 2 7 20
Observed Proportion Responding
0.0000 0.0000 0.1000
0.3500
1.0000
Proportion Responding Adjusted for
Controls
0.0000 0.0000 0.1000
0.3500
1.0000
Chi - Square for Heterogeneity (calculated)
Chi - Square for Heterogeneity (tabular value at 0.05 level)
JRA97134
Estimated LC/EC Values and Confidence Limits
Point
LC/EC 1.00 LC/EC 50.00
Exposure Cone.
18.980 50.460
95% Confidence Limits
Lower
Upper
9.860 41.719
25.898 61.212
s
D-106
US00001287
C U A ST .U . IOAN.VLYSTS. INC. NPDES STATIC ACt 'T K, SAJ.TWATKR
(Forni ACV4X)
% E ffluent
Day 1 1.0. I Live
Day 7 I liv e
Final \ S u v rv a l
C ontrel 5 .2 5
C l io
1
fc -C
L lC 7 ' Q_______ ________ __________
(o1 1
Iff
.
__________
17 r 0
IV
12.5
2-1 i 2-2 1
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16
|t > G
3-1 l 25 3 7 iff
t a ______
i ________
lO fc
4-1 50 4 7
5-1
100
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\
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. CH
(o ( oc
Tim e:
/o /r ._
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C v o n n o d o n v a rie o atu s
Sona:
S f- A & S
H ir.h n ji^ T in : j 0 /
---------------------------------
A ccluration w ater. HW M A SW 0
O th er
S e h " r..3
.
A cclinafiun T ant). trance):
^
to r i O . Li)
F w d n o onor ta lest:
A nerrw
^
(i O th e r.
fee d n i i n n j test: Not fad 0
A rtensa
/d O th er
C h an g s : 3 0 0 n iO
5 0 0 ni
1 0 0 0 .N E J
O th er
T n t v o lu m e : 2 0 0 ni
2 S 0 trf
5 0 0 ni 1 3
O th er
No. rac cetes:
M
ri
ftio to aen o d 1 6 1 :8 0
O th er
CPT IIP- D e U 1 1 f < ~ }
R arefeN iuattontem ofata
T W aler
( C f i S ' _________
T im e A n im a is A t k b d 1 ~~U> ^
S .(U B 8 y p r 7 / C ^ f
INS m uM E N T A IIO N USED:
i*m T h rn n w a ite f Pfcerber
f
D T S 0 .0 . M eter 1 ( S 8 0 h 1 t5 )
Q CP D i u n ie & M eter (0 9 5 0 0 5 6 3 )
S in o n us H I6 0 P B alance (4 0 0 2 0 0 9 3 )
O T S SCT 3 3 M eter(H 8 0 1 6 7 9 2 )
Q T S 0 .0 . M eier > 2 ( 9 B U 0 2 6 3 2 9 ) f 1 C orrang 2 4 0 pH M e ter [3N 52H )
SP P o r t t M eter (8 5 2 3 6 0 ) [ 3 S p e rtm A T C R efractom eter(A 366}
Oay 0
Oay 1
Oay 2
C ' M . f
O o .l
1 . 0 1
6. A
a n. ;
ad a/ 1 E
2 Tt
3 Z> 6
a H . M
p3
r*
acl
4 i n # 3 P 1 4.9
5
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7.7 \
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7 -U 7 -V -
n - . i0.0.
2
7- l____
(m l) 3 X . *
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4 7 .f
S H.O
7 - fn
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S
C "i r
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7A 1
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3 c . ^A
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^
3 i>
N 3 a /\ 4 a6
30 A t>
a (L --
3h
5 Replicate f
Initiais:
3
3 r>
/ -7
ili r _____
._
_0
i- /f
NOTES:
Q uality A ssurance:
A Q u J t t y A s s u m e o H tc w Iv t h u t e s t l c ft* fy t h a t o s ig r v f ic a n t
d rv n ao o n s fre n i a c c e p te d DfUturtf occiha!wv) routini impfctwns
(unlM S th u w w * n u tv d b*lw> n d lh - t
-e c u a e rly retlecf t t -
t e s l it w a s p ir to m w d by CBl.
Conw m s
--
TEST 1 D D-107
/ US00001288
COASTAL BIOANALYSTS INC.
EFFLUENT/DILUTION WATER DATA (FormEKDL0195)
INITIAL (ARRIVAL) EFFLUENT CHARACTERISTICS:
Sam ple Date l i ' / Qmi Temp. (C) 13.) 1
Conduct. (uMHOS) 1 | U* | 1 Salinity (g/kg) I I I
!______L _ I1
II II
PH I1T. '? I I I
Diss. Oxygen (mg/1) I II.f
II
I ITot. Chlorine (mg/1) L D C _ I I
NH3-N (mg/1) I $.(jy I I I
Hard (mg/1 CaC03) I M I I ! ...
AJkln (mg/1 CaC03) l ' K I
I
Color/Appearance1 K X
I
.I
I
Initials L i t i __ !______!______I
I I I
I I I I I I I
I I
FFF1 IIFN T A D JU STM EN T/PO ST-A D JU STM EN T M EA SUREM ENTS:
Date
|
1
1
1
1
Temp ( C )
|
1 11
Salinity (g/kg) |
1 11
D O (mg/1) | 1 1 1 1
Aeration Rate | 1 1 1 1 Aeration Time (min) | 1 1 1 1
Adjust. D O. (mg/1) |
111
pH | 1 1 1 1
Tot Chlorine (mg/1) |
111
1Initials
|
111
D ILUTIO N W A TE R CH A RA CTERISTICS:
DMW:
HWM: /
Date
|
1 1 1"
Temp. (C ) | Conduct. (uMHOS) |
11
11
r i iI
Salinity (g/kg) |
pH |
Diss Oxygen (mg/I) |
Hard (mg/1 CaC03) |
Alkln (mgA CaC03) |
Initials
|
1i 1
1 1. .
11
'
1 1I 1
1 1i i
11
!
1 1 1 1 1 1 1 1 1 1
1
1
1 1 1
1 1 1
I I I II I
I II II II
I
Apthip V-Vprv Muchi
ACUTE
III K / 3 m
1
1130
1 lie n
1 II 1 II 1 II 1 II
1 H /V C ?___
1 IlC -tf
ACUTE
1
1
1 II \yP l
1 II ____
1
ii^ .i f II
1 I L - g _____
1
EFFLUENT ID:
1
i (lip
TEST ID: T ^ V? Z| > * 3 ^
D-108
>
US00001289
Facility O ld L
Ad.d.ress
Toxicity Test Sample Chain of Custody (Please complete all information)
\Q Y ). L( Kl\\f-PrQ itlM
I. , . V. fAAr '
J
(,3 3
County_ N'PDESi
Pipe/Outfall/Location Instream Waste Cone
Sample collected by fprint&signk j J o ^ O n
A ffilia tion 0 V \ i gihV. b n t U ?Io
Type of sample_!(l_(Grab):
Date
Time ~7` \3^ 1^rYS
~~~ (Composite): From Date ~
Time *--______
To Date______ -- ' Time --______
Subsamples comprising composite:
Number___________ Frequency o f collection_________ Volume.
"Temperature of sample in sample collection device ^ 0
Final temperature o f effluent at sample collection point S.G 3 (L/
"Is sample collection device chilled?
Is. sample packed on ice for shipment?
`It is required that all samples remain at 0 - 4C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency (Do not freeze.')
[s the sample chlorinated?
cechlorin3ted?
I f so, how?
___________
Permit with interim chlorine limit?
~
If yes - lim it (m g/L)______ ~ ____________
Field pH ~ 7 -^l
Field Total Residual Chlorine____________
Comments/Sample description A FFF
Type of test(s) to be performed
~ <3.
-l QQ V A plO C O flA
M -LV po a m e r -$ < 2 !,
]
!
1C t / A b r it f l p . C r U jIY i C C ^ O fl
O
(Specify organisms)
\O S \
__________________
Method of shipment p-W6<~j ^ C X (
Print & Sign Names 1. Relinquished bv {~V. \ w ~ U A A X Date.
Time \ Q ' - C Q r O
Received bv_..
Relinquished bv _ 0 CM
Received bv -r-
_ A z1 _ 3 3
__________ Date \ l ( 3 '______ Date U (<3 f 5 *)
_ . Date C\j (5> ///"?
Time l o Time U -? h>
Time
FOR REED LAB USE O NLY
JRA#
Arrival Temperature : '______
Color__________________ _____ Odor________________ pH_________ DO (mg/L). ____ Conductivity(umhos/cm)_
Salinity (ppt).
TRC (mg/L)______ Mcthd_________
On ice?
D-109
Xm-r :/ 1a/V-i
US00001290
R c v 'J ' L
--
Acv-
ji- s - f- i
L 7
1 2 * <_> f-J lt
i
D-110
\
US00001291
CHAIN-OF-CUSTODY RECORD
E N V IR O N M E N T A L
& ANALYSIS REQUEST FORM
PWC ENVIRONMENTAL LABO RATORY CODE 930 BLDG Z -140 9742 MARYLAND A VENUE NORFOLK. VA 23511 - 3 0 9 5 `
PH: (757)445-8851 FA X : (757)445-8852
CLIENT INFORM ATION
COMPANY/COMMANP: ( V
contact: T ^ - 7
P H O N E : (n
g ] EXT:'
S IG N A T U R E :
O
....
CODE
F A X : ....................
...................................
......................................
TUKHAHOUHD (D n>!.
COMMENTS:
(FOR RU SH TURNAROUND STA T E R EA SO N BELOW )
SAM PLE DISPO SAL: ( )R ETU R N TO C LIEN T ( )D IS P O S A L BY LAB
R E G U L A T IO N A PPLIED:
RCRA ( SDWA ( CWA ( CAA (
) ) ) )
HRSD ( TSCA ( PHOTO ( OTHER (
) ) ) )
' m R U S H S A ^ L e a r I I u BJEc A o SURCHARGECfSS" /?K F " TME 'P R 0 C S S , N G A N D A N A L Y S / S O F S A M P L E ( S ) .
SAMPLING/COLLECTION CHARGE: POSSIBLE SAMPLE HAZARDS: COMMENTS:
EOUMBER!-----
C O N TR A C T LAB:-
INIT.: DATE: '
C O N TR A C T NO.(S)? OFFICIAL U SE O N LY..............--
US00001292
CLIENT:
Navy Public Works Center
ATTN: ADDRESS:
CITY:
PHONE:
FAX:
,
Merrill Anderson-Ashcraft Code 930. Bldg Z-140 Norfolk. VA 23511 445-8850 445-8852
SPECIAL NOTES: Contract *: N00187-93-D-9748
Delivery Order #: 260 I.O.S: 1252001 Item #: 0002FM, 0003AD PWC #: 80249
SAMPLE ID: Bldg 566 SAMPLE NO: 97-12744
Pro. Parameter
None
Fecal Coliform
c SUBMITTED BY:
James R_ Reed & Associates 11864 Canon Blvd.. Suite 103
Newport News, VA 23606 (757) 873-4703 phone (757) 873-1498 fax
SAMPLE RECEIPT DATE:
11/07/97
TIME:
1230
;; RECEIVED BY: GRAB COLLECTION
VAS 11/07/97
COMPOSITE COLLECTION:
Start Date ;
: Start Time
GRAB TIME:
End Date
1135 End Tune
COLLECTED BY:
VH - Navy
PICKED UP BY:
NUMBER OF CONTAINERS:.
- Bayside 2
: EXPLAIN -'v; ':.y i
!
/ Condition
_X_GOOD OTHER
Method Number.
JRA Quantification
Level*
Practical Quantitatiob : Limit
Result;
SM ISth 922IE 2/100 mL
20/100 mL
40/100 mL
Analyst/Date/Time R ES-11/07/97 @ 1325
NOTES: * JRA Quantification level is the concentration of the lowest calibaration standard above zero with a reliable signal.
RESPECTFULLY SUBMITTED BY: DATE: November 11, 1997
Elaine Claiborne Laboratory Director
D-112
US00001293
9
D-114
i
US00001295
Coastal Bioanalysts Inc.
ODU/Oceana Acute Effluent Toxicity Tests
M ysidopsis bahia & Cyprinodon variegatus Sample I D. A3
Contract No. N00187-93-D-9748 * Delivery Order No. 0235
J O. No. 1912290, Item No. 0002BJ, 0002BH PWC No. 1364
11/2/97 1755 Sample No. JRA97-12381
Submitted to: Ms. Merrill Anderson-Ashcraft
Navy Public Works Center Code 930, Building Z-140
Norfolk, V A 23511
B
By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, V A 23062
(804) 642-0168
D-115
US00001296
Sample I.D. A3 j I 2.97 Sample 97-123X1 Test ID: JRA97133
Coastal B io a n a ly s ts Inc.
I. TEST RESULTS
% Effluent Concentration
0 6.3 12.5 25.0 50.0 100
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acule Test Results
Mean (%) Organism Survival
M . baha
C. variegatus
24-h
48-h
244l 4 M
fOO 100
100 100
100 100
100 100
100 100 100 100 100 100 100 100
100 100 100 100 100 100 100 100
>100% N/A N/A <1.00
>100% N/A N/A <1.00
Acute Test Inform ation:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
M. bahia Test
48-h Static
g
Survival
11/3/97 1640
11/5/97 1650
CBI stock cultures
11/1/97
20 g/kg
20.0-20.6C
No
N/A
C variegatus Test 48-h Static Survival 11/3/97 1655 11/5/97 1655 Aquatic B io Systems 10/30/97 20 g/kg 20.0-20.6C No N/A -
'Aeration necessary when effluent oxygen demand results in dissolved oxygen concentrations < 4.0 mg/1.
D-116
US00001297
ODL'-OCEANA Sample I.D. .A3 11 Z'9? Sample 97-12381 Test ID: JR.A97133
Sample Collection Date/Time
11/2/97 1755
Sample Use Dates/Times
11/3/97 1640/16553
Aeration2 Rate/Xitne
n/a
Pretreatment/ Adjustment
HW M Salts
2Aeraiion often necessary to reduce sample oxygen concentration to 100% saturation after warming. \ \ / . hahia/C. variegatus test time.
Table 2. Effluent and Dilution Water 4 Chemical & Physical Characteristics (Mean Value (Standard Deviation))
Parameter
Temperature (C)
Salinity (g/kg)
Conductivity (uMHOS/cm)
pH
Dissolved Oxygen (mg/1)
Total Hardness (mg/1 as CaCOj)
Alkalinity (mg/1 as CaCOj)
Total Chlorine (mg/1)
n h 3-n (mg/1)
Initial Effluent
3.4 (-)
1 (-)
1659 (-)
8.17 (-)
11.5 (-)
122 (-)
303 (-)
<D.L. (-)
4.7 (-)
Adjusted Effluent
20.3 (-)
21 (-)
N/A
Dilution W ater
20.6 (-- )
20 (...)
N/A
7.98 (-)
8.0 (-)
i N/A
7.83 (-)
7.9 (-)
N/A
N/A N/A
N.D.
N.D.
N.D.
.
N.D.
--
.
4Efflucnt parameters measured upon receipt of sample in lab and after adjustment on day of use. Dilution water (Wimex hw-M;irincmi.\ in ASTM Type I deionized water) values measured after preparation of diluent on day of use.
D-117
US00001298
COASTAl. HIOANALYSTS. INC. NPDES STATIC ACUTE/SAI.TW a TF.R
(Forn ACV4X)
X E ffluent
Day 1
L 0 . 9 Live
C -l C-2
6 .2 5
1-1 12
1 2 .5
2! 2*2
3-1 25
3-2
4*1 50
4-2
5-1 100
5-2
O bserver:
Tim e:
, ! (.
;' c
10
til '0
11>
16
Ih
l(,
U>
ib
GfJ iCa 35'
D ey 2
9 Live
to
10 16
16 '
Final \ S iev rv al
0
l 0 t>
0
to 1 d
to iO
16
to
I 00 \ 00
}<>
'0 G-ff1U T <
,. { O O
S p e c ie s:
C y p r i n o d o n v ? r i e q a t u s __________________ S o u r c e :________ $
Hatch QatVTime: f >/ 3 o / A T ____________________
A ccliiT etion w a t e r HW M A SW p
Q th ec^
re*rQjLi
X U AcclTBtion Temp, [range): >
-
to JL ( n
F e e d n g prior to to st:
___ / d O tf w r .________________
F eed n g A m ng te st: Mot fe d
A r t e n # /d
O ther.
*0005 0 0 ni
ErH
O tf-r
2 5 0 ri Q
rrf
fQ
N i n d o tit ^
ft to p en o < t t6 L :8 0 W
)tW ~.
R a n fe n ttm tan y M a
\ M f A l -SET U P: D ate__
_ T im e W a t e r A A h d _ l l i l _________
T u A ram eli A dded l \ j S V
S et Up By ( / " f f
INSTRUM ENTATION USED:
S u m T herm om eter N urrtaar /t f YSI 0 .0 . M e ter 9 1 { 9 6 B 0 1 4 4 7 5 ) E 3 C P D i s s e n s # pH M e t e r ( D 9 5 0 0 S S 3 )
S a rto m s R I5 0 P B alance (4 0 0 2 0 0 9 3 ] Y S SCT 3 3 M eter tH 8 0 1 6 7 92)
Q Y S 0 .0 . M eter 2 (9 S 1 A 0 2 S 3 2 9 ] C o n i n g 2 4 pH M e t e r ( 9 6 2 6 8 )
SP P o r t* * 2 M eter (8 5 2 3 6 0 )
Gj.S p e i ) A T C f b f n c t o m t e r (A 3 6 6 )
O ther
O ayO
Day I
D ay?
C s c .V
M .T
1 ri C
f < V . 1
2 Q r , y . . / < ( . (r
3 u ,v 4
n .i h%
5 10
c Iti* 1 l 80
2--U f
A oA c^o .T A t PT
3 1 .T Au<=r e lli V
pH
2 7 - 5 '?
f- n
G .? }
3
T -.lfo
M *3
4
8. e A
S J. * 3
0.0.
(ml)
S A
L l
N
5
C
1 2 3 4 :5
;c
1 2 3
M S' M
M M TA IA o
A) Ah QA ^
% , o <! ^ 3 <
M l fr J} 'M ,
( - > r. r. 8h
*-U
~ IA , > to T .V ?S ' =3b A t, 1h G^
(9*9) 4
5
cate 9
<3 o
A1
i
'.l c-
a
j
3/) O
liais: _ L i d _____ ( H
(r^
NOTES
Q uality A s u m c i :
A* Q uehry A u ax e n ce o fficer for t i n te s t 1 c ertify that no significant d e v w tto m from a c c e p te d pro to co l o c c u rre d during routine in sp e ctio n s (un less oth erw ise n o te d below ) and th a t th e d a ta aec w a ie ty refle ct th e ta e t a s it w a s p e rfo rm ed b y CB1.
C orn n au ti
r r-
)/)
&9W e
c < -J C O
. h f l A
0
D-118
TEST I D.: / J lA j
j
US00001299
COASTAL BIOANALYSTS INC. EFFLUENT/DILLTION WATER DATA (Fom EFDL0195)
INITIAL (ARRIVAL) EFFLUENT CHARACTERISTICS:
Sample Date i u m i
i
Temp. (C)
1
Conduct. (uMHOS) 1 ' ( r f !
1
I
Salinity (g/kg) | |
i1
I |
1 1 1
11 11 1 'I
||
pH i f f . 1 7 i
i 11 1
Diss. Oxygen (mg/1) i i i . r i i i 1 1 1
Tot. Chlorine (mg/1) c O C 1
1
1
NH3-N (mg/1) I V , > 1
1 1 1I
I
Hard (mg/1 CaC03) 1 0 3 1
1
1___ 1
1
1
Alkln (mg/1 CaC03) 1 ^ ^
1
1
1
11
1
Color/Appearance1 1 ^ 1
1
1
1
1
1
1
Initials
Lw 1
1
1
1 .1
1
1 C-Clear O-Opaque Y-Yeilow B-Brown Bl-Black G-Green T-Turbid SI -Solids (S-Slight M-Modcrate V-Very Much)
EFFLUENT ADJUSTMENT/POST-ADJUSTMENT MEASUREMENTS:
Date
|
1I
11
Temp. (C) | 1 1 1 1
Salinity (g/kg) | 1 1 1 1
D O (mg/1) | 1 1 1 1 '
Aeration Rate | 1 1 1 1
Aeration Time (min) | 1 1 1 1
Adjust. D O. (mg/1) | 1 1 1 1
pH | 1 1 1 1
Tot Chlorine (mg/1) | 1 1 1 1
Initials
|
1
1
11
1 1 1 1 1 1 1 1 1 1
ACUTE 1 ll/i/im 1 Il Q M
II 0 f 1 II9 n 1 II -- 1 11^ 1 II -- 1 > .1 9 1 H/VC7___ 1 Il O H
DILUTION W ATER CH A RA CTERISTICS:
Date
|
I
I
Temp. (C ) | I I
Conduct. (uMHOS) | I I
Salinity (g/kg) | I I
PH |
Diss. Oxygen (mg/l) | I I Hard (mg/l CaC03) | I I Alkln (mg/l CaC03) | I I
Initials
|
DMW:
H W M s/
III III III III III I I .I III III I
ACUTE
llw v n WQsb (o
II^
II 3 a
ll> 9-3
l> .)
II
Il h/R
II H f-
E F F L U E N T ID: 1 i>
_T E S T ID: D-119
^
US00001300
T o xicity Test Sample Chain o f Custody (Please complete all information)
Facility O lc b O m t V i t O n U n u f ^ r O i - h ^ j
Address
VI . VAr ^
~
County, NPDESr
Pipe/Outfall/Location Instream Waste Cone
Sample collected by (print&si.gnk y J o ^ O n
A ffilia tion ODlJ riVw. y.y\ r t L?U
Type o f sample ^ (Grab):
Date , |,/ V ^ ~ y Time *7: 5 5 'n ' *
^
.(Composite): From Date.
Time
To Date__ -- Time --
Subsamples comprising composite:
Number___________ . Frequency o f collection._________ Volume,
'Temperature o f sample in sample collection device 0 Q j
Final temperature o f effluent at sample collection point r2 \ % &
'Is sample collection device chilled?
Is sample packed on ice for shipment? vj&S
'I t is required that all samples remain at 0 - 40C during collection period and shipment fo r
data to be accepted by the appropriate Regulatory Agency (Do not freeze!)
Is the sample chlorinated?
dechlorinated?
If so, how?
_________ __
Permit w ith interim chlqrine lim it?
~
If yes - lim it (mg/L)______ ~ ____________
Field pH l . \ Z
Field Total Residual ChIorine___________
Comments/Sample description, A 3 -
U-terQ op CjO(\\cvL____
Type of test(s) to be performed_ (Specify organisms)
Method o f shipment, peuver^
\f<Xf\
Q V\A,
< 3 n n rrtp . C c u c W n O g lo ri ________________________
P rin t & Sign Names
I. Relinquished bv P r. r \C .Y\ -
Date
/O H rime \ Q - G Q a r t
Received bv
Date f
Time 1 0 hCT
Relinquished bv \ )
Date
^ T ime I 13 & _____
Received bv
Date tA V th rY y
Time, . i i l Q .
/^ 3o
JRAZ ^ 7 - / ^ 3 ? /
FOR REED LAB USE O NLY A rrival Temperature__________
Color_______________
_____ Odor_______
PH________ DO (mg/L)_
Conductivity (umhos/cm)_
Salinity (ppt).
TRC (m g/L). _____ Method_________
On ice? Solids.
@ _______ C
D-120
Rev 3 / l i / 94
Vn t.
US00001301
\e v < $ i_ d L ^ .
feyJr: ^ 0 0 X
f)J
& 3o
11-5 - < n
c f i/ t f
/?**
8
8
D -12I
US00001302
CHAIM-OF-CUSTODY RECORD
E N V IR O N M E N T A L
& ANALYSIS REQUEST FORM
PW C ENVIRONMENTAL LABORATORY CODE 9 3 0 BLDG Z- 14 0
9742 MARYLAND AVENUE NORFOLK, VA 2 3 5 1 1 - 3 0 9 5 '
CLIENT INFORMATION
C O M P A N Y /C O M M A N D : f V
CODE:
& I A T . ^ . | aI L Z ^ T , --------- r' ~ ' .
P H O N E : \p X
E X T ; "' '' " ' FAX:' " ........
JQ#:
I 1 1 A J A '\ O
S IG N A T U R E : 0 .-v h '- Z
"
LAB USE ONLY LINE ITEM # ISAMPLE NO SAMPLE ID/LOCATION
~ 'l - T 'i 1` 1 L "
JAM/U TAH
STAPF
OH
OATS
AT TTPg SAHPUO
WO f SAMPLES
TIM ST HAIfill PRESERVATIVE /CONTAINERS
ANALYSIS
____ PIEJ-D READINGS I pbcservaIk'
(30 0 JA B T
F * * e i C 7 'O r- l<L_
J y t T /'/A 'i 7?
( 3 k S R'i/iX
i?,7k hr
STOP tH /A ^ i | : /0 STARI
STOP STAAT
O'lco
G
^"T
L
/
; P- -
l Ti o^ /Fy u'f ; | i y;
.... L ( - X ,D D , J' J /- X
Uhl
b I STOP b i l S ' STAAT
Z Jj o L HfTrV
STOP o&'co
I7-/A j a o
STAAT
J 3 t f Z l rtffir#
STOP o ' / i r
_ _ *7'/A'SV /
I S b . H V^F 3 - C o a V o (
STAAT STOP
'f.iZ
CH
.... -- SJ
STAAI
STOP 5 3
......................- f e t--
.
...... '7-So J / . y
-i 7 / 1 '
_ f) . V J O . S Z, 7aL Jn h} S 'x col o . 9
--
G - GRAB CF-COMPOSITE, FLOW CT-CO M POSITE, TIME
L - LIQUID. SW -SW IPE S -S O L ID G S -G A S S S -SEMI SOLID
P-PLASTIC
GL - GLASS T-TEFLON
V-VOA
CONTAINER
8 -BAG C - CARTRIDGE
A AMBER(glass)
TL-TEFLON LINED LID
H-HEXANE RINSED
T U R N A R O U N D (Dit i): CO M M ENTS:
(FOR RUSH TURNAROUND STA TE REA SO N BELOW)
S A M PLE DISPOSAL: (I )R E TU R N .TO C LIE N T ( )DISPOSAL BY LAB
REGULATIO N A PPLIED:
RCRA ( SDWA ( CWA ( CAA (
) ) ) )
HRSD ( TSCA ( PHOTO ( OTHER (
) ) ) )
P R E S E R V A T IV E
1 COOL TO 4 C
5 HCL, pH<2 1
2 - HN03, pH<2
6 0.008% Na2S203, 4 C
3 - H2S04, pH<2 7 - FIELD FILTER
A - NaOH, pH>12
8 - NONE
I SAMPLING/COLLECTION CHARGE: $
POSSIBLE SAMPLE HAZARDS:
COMMENTS:
........ .......
TO HUMBER:--
CONTRACT LAB:-
INIT.: DATE:'
CONTRACT NO.(S)
'O F F Ic a r V S F D N L V ----
" ,HWS' Am * * t f * ' * * r n m i m m ,^ J i ^ s s s s s s
REJECTED ( )
R E A S O N : HOLDING TIME! i
n o M T A iM c o ^ ^ 3
______________
U S 00001303
Coastal Bioanalysts Inc.
ODU/Oceana Acute Effluent Toxicity Tests
M ysidopsis bahia & Cyprinodon variegatus Sample I D. A2
Contract No. N00187-93-D-9748 " Delivery Order No. 0235 J.O. No. 1912290, Item No. 0002BJ, 0002BH
PWCNo. 1365 11/2/97 1730 Sample No. JRA97-12382
Submitted to:
Ms. M errill Anderson-Ashcraft Navy Public Works Center Code 930, Building Z-140 Norfolk, V A 23511
By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA23062
(804) 642-0168
D-123
US00001304
ODI "-OCEANA .Sample I.D. A2 11 2 97 Sample 97-123K2 Test ID: JR A 97132
.f I. TEST RESULTS
Coastal Bioanalysts Inc.
% Effluent Concentration
0
6.3
12.5
25.0 50.0
100
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acute Test Results
Mean (%) Organism Survival
M . baha
24ih 4 M
C. variegatus
2M Sd
100 100
100 100
100 100 100 100 100 100 100 100 100 100
. 100 100 100 100 100 100 100 100 100 100
>100% N/A N/A <1.00
>100% N/A N/A <1.00
/
Acute Test Inform ation:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
M. baha Test 48-h Static Survival 11/3/97 1650 11/5/97 1630 CBI stock cultures 11/1/97 . 20 g/kg 20.0-20.6C No N/A
t
C variegatus Test 48-h Static Survival 11/3/97 1650 11/5/97 1640 Aquatic Bio Systems, Inc. 10/30/97 20 g/kg 20.0-20.6C No N/A "
'Aeration necessary when effluent oxygen demand results in dissolved oxygen concentrations < 4.0 mg/1.
!i
D-124
US00001305
O D I' nCl-ANA Sample I D. A2 112.97 Sample 9 7 -123X2 Tesi ID: JR .W 1 3 2
Coastal Bioanalysts Inc.
Sample Collection Date/Time
1 1/2/97 1730
Sample Use. Dates/Times
11/3/97 1650/16503 *
Aeration2 Rate/Time
n/a
Pretreatment/ Adjustment
HW M Salts
2Aeration often necessary to reduce sample oxygen concentration to 100% saturation after warmtng. 3.1/ bahia/C. variegatus lest time.
Table 2. Effluent and Dilution Water 4 Chemical & Physical Characteristics (Mean Value (Standard Deviation))
Parameter
Temperature (C)
Sal initv (g/kg)
Conductivity (uMHOS/cm)
pH
Dissolved Oxygen (mg/1)
Total Hardness (mg/1 as CaC03)
Alkalinity (mg/I as CaC03)
Total Chlorine (mg/1)
n h 3-n (mg/1)
Initial Effluent
2.8 <-- )
1 (-- )
1603 (--)
8.19 (-)
11.2 (-- )
126 (-)
271 (-- )
<D.L. (-- )
5.2 (-)
Adjusted Effluent 20.1 (...) 20 (--) N/A
7.98 (-) 8.0 (-) P/A
N/A
N.D.
N.D.
Dilution W ater 20.6 (...) 20 (...) N/A
7.83 (-) 7.9 (--) N/A
N/A
N.D.
N.D.
4E(Tlucnt parameters measured upon receipt of sample in lab and after adjustment on day o f use. Dilution water (Wimex hvv-Mnrincmix in ASTM Type I deionized water) values measured after preparation of diluent on day of use.
D-125
US00001306
0D I'< X T E A N A Sam ple I.D. A2 w n o l Sample 97-12382 Test ID: JRA97132
Coastal Bioanalysts Inc.
fl. W ATER Q UALITY
Parameter Temperature
(C) DO (mg/1) pH
Sal initv (g/kg)
Parameter Temperature
(C) DO. (mg/1) pH
Salinity (g/kg)
Control
20.4 (0.2)
7.8 (0.1)
7.73 (0.10)
20 (0)
Table 3. Water Quality Summary
M . bahia Test
Mean Value (Standard Deviation)
2%
12.3%
25%
50%
20.3 (0.2)
20.2 (0 1)
20.3 (0.2)
20.2 (0.3)
7.8 (0.2)
7.77 (0.08)
20 (0)
7.8 (0.1)
7.82 (005)
20 (0)
7.8 (0.2)
7.92 (0.01)
20 (0)
7.7 (0.3)
8.03 (0.07)
20 (0)
100%
20.2 (0,1)
7.7 (0.3)
8.13 (0.15)
20 (0)
Control
20.2 (0.4)
7.8 (0.2)
7.76 (0.07)
20 (0)
C. variegatus Test
Mean Value (Standard Deviation)
6 3%
20.2 (0.4)
7.8 (0.1)
7.78 (0.07)
20 (0)
12.5%
20.1 (0.2)
7.7 (0.2)
7.82 (0.07)
20 (0)
22%
20.0 r.(0.3)
7.7 (0.2)
7.87 (0.05)
20 (0)
50%
20.1 (0.3)
7.7 (0.3)
8.00 (0.05)
20 (0)
100%
20.0 (0.2)
7.7 (0.3)
8.12 (0.13)
20 (0)
D -I26
ii
US00001307
O D f O C EA N A Sam ple I. D. A2 1 1 . 2 97 Sample 97.123X2 Tesi ID: JRA97132
III. OUTFALL DATA SUMMARY
Coastal Bioanalysts Inc.
Test Date
' 9/30/97 11/3/97*
Table 4. Cumulative Data Summary - Acute & Chronic Tests (Tests conducted on this outfall by CBI)
Invertebrate
Acute Tests
Chronic Tests
LC50 XJLL
NOF.C U L
>100% <1.00 >100% <1.00
N/A N/A N/A N/A
V erteb rate
Acute Tests
Chronic Tests
1X50 U L
NOEC U L
>100% <1.00 >100% <1.00
N/A N/A N/A N/A
Results submined with this repon
IV. QUALITY ASSURANCE
M. bah ia - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
C variegatus - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C L ): Control Chart LC50 (95% C.L.):
CBI Stock Cultures 10/15/97-10/17/97 KC1 (Sigma "Ultra" Lot#76H10391) 343, 490, 700, 1000 mg/1
100%
574.2 mg/1 (540.9-609.5)
612.1 mg/1 (488.3-735.9)
Aquatic Bio Systems, Inc. (same batch as test) 11/3/97-11/5/97 KC1 (Sigma "Ultra" Lot#76Hl0391) 432, 720, 1200, 2000 mg/1 100% 1200.0 mg/1 (1070.5-1345.2) 1074.9 mg/1 (891.2-1258.6)
D-12^
US00001308
CUMULATIVE DATA SUMMARY NPDES Permit #: N/A Oceana - ODU A-2
D a te o fy layeribratesl^y V y
Testy y L 5 0 2 5 S
;- y ;
2/12/97 >100
>100
2/28/97 Invalid
>100
3/12/97 >100
>100
3/20/97 >100
>100
3/26/97 >100
>100
9/30/97 >100
11/3/97 >100
>100
> io o
jfriYertebrt^yy:;;
N O E a % : y y - --. =;
------
-
*
Supplement
D-128
US00001309
COASTAL 8JO/VNALVSTS. INC. NPDES STATIC ACUTE/SALTWATER
(Form AMB48)
\ Effluent
t.O.
0yl 9 l/ve
0y ? 1l/ve
Control
C- i f C-2 ' C
'0 '!
6.25 1-2
2-1 12.5 2-2
3-1 25 3-2
4-1 50 4-2
S-l too 5-2
Observer Tit:
' It
f{/
ib
10 >(, If' f
10 ic 16
/
tv
'0 10
'V
to
w
lu
r.f /-
C .-H
____ /lo 3 o
Bruii SuTVTvW ) &fc l0>
l 0 t 6c
J to
S tacies:
M y sid o p s is b a h ia
O a l W
/( f i f ?
CBI
Acclimation w i i i r HWM ASW
Othar._______________ S alm ry X ? A
Acclimation Temp. [ r a r q m Y 3 t > . 0
F eed n g p w to te s t:
A riern! ^ ^
__ X O < ^ D . L ? ____________ / d O tte r
P eerin g during te s t: t o t fed
A n im a
/ d O ite c _
tem ber site: 3 0 0 ^ i 2
5 0 0 ni Q
1 0 0 0 ni
I a t i v o i m a : 2 0 0 ni
2 S 0 ai Q
5 0 0 ai
I n t u m. o r g a n a r p a r r e s c a t e :
/ >
No. /e d c a te e :
F te to p e n o i 1 6 l:B 0 a
O tte r
R a n d o m t a o n t m p l a t e n a . ^ *" |
SET U P : D M t f i / 3 m
V_ T u m W a u r A rfchd ( B
Q
Tana A ia r d t A d ted
I Lp
SwpSv t r - M ) ! ? &
INSTRUM ENTATION USED:
Stem T h afim ra tar M u n ir A } YSJ 0 .0 . M a la r * I ( S N E B 0 1 7 5 )
I CP D < 9 m * # M alar (D 9 5 0 0 5 6 3 ) I S a n onus H I BO P Balana (4 0 0 2 0 0 9 3 ) Y S SC I 3 3 M asar (H 801E792)
Q r a 0 .0 . M alar 1 2 ( 9 6 1 4 0 2 6 3 2 3 )
CorrvnQ 2 4 0 pH M a la r ( 9 6 2 6 8 )
SP P o n a t* 2 M atar (8S2360) [ 3 Scartar. ATC R a f ra c u n a ta r (A 36S)
O ther
OayO_________ D*y 1
0*y 2
C 0) b 1
2 . l y - 7?
3 30 t
4
S A - /
C
. ^ A .y _ _ _ _
io y 3 .r 3 C .T
1 3 ~ lr
2 z .z y 3 fj? ? ^ r P
0 0 fi Xf; /
J t,.)
XD 7 ri U
n ,1 7 -lo V r Z i) 7 .^
? .* 3
4 & .P s
s
sv y
^ .r)?
c M
2
X -? 9-ff
2 .3
T -b 1 -.7
3 7 -.</ z-
1-
4 Ko 5 K. C cQ o
zs7 -v c2 A
Xb 1 Le -O
Q fi
-Jo
3
4
5
cat 9
tia lr
<A /S rO Ao
D
Oh
A
0
3D
io <X D -i
<3
H i ____
NOTES
t-
Q uality A u u v a :
A s Qualry A ssu ran c a o ffic e r for tN $ te s t 1c e rtify th e t n o s i n fic a n t A w tatio ra from a c c e p te d protocol occurred d e in g routine inspections (u n les s o tte n e r s i n o te d telo} a n d th e t i t e s t a a c c u ra te ly refle c t th e ta c t a s tf W H p e rfo rm ed b y CBI.
C orrvnents
.A .
J . SignM ui
VU
a
i (nhl
0e
TEST I D.: J n / 7 * i 7 / ) 2
D-129,
US00001310
COASTAL BIOANALYSTS. INC. NPDES STATIC ACUTE/SALTWa TER
(Form ACV4S)
*fH urrt
Day 1 l.D .
/ LlV*
Oay 2
$ Ljv*
6.2 5 1 2 .5 25 50 100
C-1 1 L-
C -2
! (> 1*1 t
'0
I
I
1-2 1 0 2 -1 I (j 2 - 2 ( c
f G3-1
3 -2
________L L __________
4-1 < 0
4-2 ' G-
5-1 1 l>
>0 10
Ip
'0
0 <0
10
IO
5 -2 O bsarvar
1b C-H
t L>__________
C - //
T im e:
iG
_______
Final \ S uvtval
1C o
1 O
1Oo ( OO
l 6 '0
(O O
\r r r
fy n rin o d n v a rie q a tu s
Score:
C S F /^ J /^ 3
A cclvratron w a te r. HW M ASW
O tte r
$ lir* n rc^ A
A crlirratio r Tem o. frano):
. Cl
F e e n q p rw to ta ti:
A rtanw X
t u S D , [<f
/d O th * :
Feadng dunng t n t : N ot fad Q
A riana
fi O t t e r
C t o i w , . : 3 0 0 iri
S00n4
1 0 0 0 fri H
O tte r
(ri
Th i lu m e : M O ^
2 S 0 rtD
5 0 0 IT* H
O lia r
n4
In h al no orearasns par m h c a te :
fO
lb n d o ta c O
P te to p a n o 16L:BD
O tte r
R an tb rn zstio ft tam cJat* no. ^
j^
SETUP: 0 . I l r l / ^ i 9 -
lim a W a r A ddad / L i V
T u r A n*maJs A ddad | C i ) S " C 1
S a t U d By r~H / 3 ^
INSTRUM ENTATION USEO:
S lam T te m w a ta r N u rtn c A |
G Y 9 0 .0 . M a ta r f 1 (SNEB14 ^ 75) G C P D iy jaro a [H M atar (0 9 5 0 0 5 6 3 ) S arto m s R i 6 0 P B alarr (4 0 0 2 0 0 9 3 ) Y S JS C T 3 3 M at# r(H 8 0 1 6 7 9 2
Y S D .O . M a ta r 9 2 ( S 1 A G 2 6 3 2 S ) L J C orri#* 7 4 0 & M a tar ( S 2 6 8 )
$P P b rta fJtt M a te (852360) S partan ATC R a fn c to m e ta r (A 366)
O thar
0T 0
0y I
C 0 t* H . f i
T 1 < r * 7
2 3*s? - 3
n
3 p
H.7
4 )
itti
5 7*9.)
.i
C 7 .r ? 3
I ?-
7-7-
2 t f r 7-, 7 7 fH
3
7 - 7 fi l - K t -
4 7-3 l
5 i - n 8 , >
c ? -.< ?
f .k
I 7- ?
7 --?
D .O . (m g4)
2 IL
3 ~7--1 1-(n
4
ft.f)
5 t7 )
7 Ir
s C J?A
A
I 7) h
o? A
L
2 30
ol 6
N 3 AA Ap
4 3
ol b
5 R alicat f
36 )
Qh 3.
In itia it:
_ L /i--
NOTES
Oay 7 - 7 0 . '7 \s 3
P :-J
T 0 .A
^ c -1 9 .7 - S '
H
Z.U
1 -^
1-3
7 u* 7H .^ 6
7 ^ 0________
^A
71? A. 0
A
( r II
TEST I P : D-130
US00001311
rOASTAi. BIOANALYSTS INC.
EFFLUENT/DLLUTION WATER DATA (Komi KDi-ui)
INITIAL (ARRIVAL) EFFLUENT CHARACTERISTICS:
Sample Date iii/ami
1 I'
11
Temp. (C) l a . i i i 1 1 1
Conduct. (uMHOS) | | U3 I I I I I I
Salinity (g/kg) I I I .
I I II I
PH 1g . 1 9 1 1 1 1 1 1
1Diss. Oxygen (mg/1) \ i l . Z 1 1 1 1 1
Tot. Chlorine (mg/1) 1LP(_ 1 1 1 1 1 1
1 1NH3-N (mg/1) S , D 1 1 1 1 1
1Hard (mg/1 CaC03) Il O U 1 1_ 1 1 1
i a : iAlkln (mg/1 CaC03) i. i i_ 1 1 1
1C_/Color/Appearance1 1 1 1 1
1
Initials 1G-fF 1 1 1 1 1 1
1 C-Clear O-Opaque Y-Yellow B-Brown Bl-Black G-Green T-Turbid SI -Solids (S-Slight M-Moderate V-Very Much)
FFFI Il FINIT ADJUSTMENT/POST-ADJUSTMENT MEASUREMENTS:
ACUTE
Date I I I ! ITemp. (C.) I I !
1 1 1 IU /3/?
11
Il2 . l
1Salinity (g/kg)
I
I
1 ..1. 1 1 list
D O. (mg/1) 1 1 1 1 1 1 I 8.0
1Aeration Rate
1
i
1 1 I-
1Aeration Time (min)
1
1
11!
1
I-
Adjust. D.O. (mg/I)
pH
Tot Chlorine (mg/1)
A -O
Initials
DILUTION WATER CHARACTERISTICS:
DMW:
UWM;
ACUTE
Date
U /4 1 .
Temp. (C)
Conduct. (uMHOS) Salinity (g/kg)
3iz)
pH Diss. Oxygen (mg/1) |
id.
Hard (mg/1 CaC03)
/V fl
Alkln (mg/1 CaC03) Initials
K /
c~/t
E FFLU EN T ID: A
ID T f l
US00001312
Toxicity Test Sample Chain of Custody (Please complete all information)
Facility Q \ c b O v r iiV lO n . U m xf-^rQ i W
Address...t O o r p o U c . VA- 3 t t p C \
^
Countv. NPDESi
Pipe/OutfalL/Location Instrcam Waste Cone
Sample collected by (pfintfeignU J c ^ O n
Type of sample f .((G"Grraabb))::
DHaarter / V ^
Affiliation PD U FrtV. Time 5 - 37,C0t nsr\
n l^ L J
,,(Composite): From Date ~
Time____ ^ _____ _
To Date______ ~~ Subsamples comprising composite:
Time
--
Number____________ Frequency of collection. 'Temperature of sample in sample collection device O CL'
Volume
Final temperature of effluent at sample collection point o U Q ''>
Is sample collection device chilled?
Is sample packed on ice for shipment? i/jg-S
It is required that all samples remain at 0 --4C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency fDo not freeze!)
Is the sample chlorinated? ^
dechlorinated? ^ If so, how? V ^ - _____________
Permit with interim chlorine limit? ~
[f yes - limit (mz/L)_____ --_____________
Field pH 7 - 5 7
Field Total Residual Chlorine____________
Comments/Sample description. k Q - U -ferQ
( lo n V r tr fy __________________
Type of test(s) to be performed (Specify organisms)
Method of shipment. Oe'bVertj
flC U
<2,VricVrtl p C c U p T i'n O ^ O fl J*
Print & Sign Names
1. \G '-C O a rO
Received bv \J n Relinquished bv \J
Date I d ^Y *7 Date M(3 /*? *1
Time Time f t 0
Received bv
JUU
A j : T T iT I I T - --
Date Vo(3
Time l l 3 0 .
______Z //9 /5 7 __
f <J~3G
FOR REED LAB USE ONLY
JRAif r 7 ' J ^
Arrival Tcmpcmrnrr
Color
Odor
pH--------------- DO (m ^L)_______ Conductivity (umhos/cm)_
Salinity (ppt)_______ TRC (mg/L)______ Method__________
On ice? Solids
\ \\
D-132
Urn-r : / 1 4 / 9 4
k-^/- L ^ j
D Jx_
X Jc
n -* -* n -- --
r r fifc i /75o-
D-133
US00001314
CHAIN-OF-CUSTODY RECORD
r
E N V IR O N M E N TA L
& ANALYSIS REQUEST FORM
PWC ENVIRONMENTAL LABORATORY CODE 9 30 BLDG Z -140
9 7 4 2 MARYLAND AVENUE NORFOLK, VA 23511 - 30 9 5 '
PH: 1757)445-8851 FAX: / 7 5 7 ) 4 4 5 - 8 8 5 2
AS L/S ONLY
LINE IT E M S A M P L E N O
C LIEN T IN F O R M A T IO N
C O M P A N Y /C O M M A N D :
c ja u k
CODE:
contacV ^ ^ : s :,:e z : PHONE: 7 3 ^ 1 : f \ EXT:'""............ " FAX:"
J .O . ;
IT M
S IG N A T U R E : O . v - f 11 I P E R M IT N O .:
u> TYPE
G -G R A B C F -C O M P O S IT E , FLO W CT - C O M P O S IT E , T IM E
TURNAROUND (D<)!
COMMENTS:
M A T R IX
L * L IQ U ID
S W . S W IP E
S - S O LID
GS GAS
S S -S E M I S O LID
P P L A S T IC G L -G U S S T - TEFLON V -V O A
C O N T A IN E R
B BAG
A A M B E R (g la s s )
C C A R TR ID G E
TL TE FLO N LIN E D LID
P R E S E R V A T IV E
1 - CO O L TO 4 C
5 - HCL, pH<2 *
2 -H N 0 3 , pH<2
6 - 0.008V , N a 2 S 2 0 3 , 4 C
3 M 2S04, pH<2
7 . F IE L D F ILTE R
SAM PLE DISPOSAL: (1 JR E T U R N T O C LIE N T' ( JDISPOSAL BY LAB
A L T R is rS A ^ fe S ^ R lT u L ^ E C T ra SURCHARGe" 5^ TM TM Pf,0CSSING AND ANALYS' S SAM PiEiS).
CONTRACT NO .(S~
......
OFFICIAL VS E O N LV "
7. SAMPLES RECEIVED AFTER 2 :0 0 PM MON. THURS. AN D 1:00 PM d N M ip A V 'w T lT B E PROCESSED THE NEXT BUSINESS DA Y I7 :Oq "a m - 3 :3 0 PM)'. '"
F O R LAB U S t O N L Y li
REJECTED( )
REASON:
S A M P L E T v RiFCf ION`
US00001315
Coastal Bioanalysts Inc.
ODU/Oceana Acute Effluent Toxicity Tests
Mysidopsis bahia & Cyprinodon variegatus
Sample I.D. A1 Contract No. N00187-93-D-9748 5 Delivery Order No. 0235 J.O. No. 1912290, Item No. 0002BJ, 0002BH
PWCNo. 1366 11/2/97 1830 Sample No. JRA97-12383
Submitted to: Ms. Merrill Anderson-Ashcraft
Navy Public Works Center Code 930, Building Z-140
Norfolk, VA 23511
2
By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062
(804)642-0168
i
D-135
US00001316
ODI.'OCE.VN Sample KD. A l l i 2 97 Sampli 97-12383 Test [D: JR.A971.il
Coastal Bioanalysts Ine.
I. TEST RESULTS
% Effluent Concentration
0
6.3
12.5
25.0
50.0
100
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acute Test Results
Mean (%) Organism Survival
M. bahia
C. variegatus
24-h
4H l
24Ji 4S4x
K)0 100
100 100
100 100
100 100
100 100
100 100
100 100
100 100
100 100
100 100
100 100
100 100
>100% N/A N/A <1.00
>100% N/A N/A <1.00
Acute Test Information:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated? Aeration Initiation Date/Time:
M. b a h ia Test
48-h Static
,
Survival
11/3/97 1635
11/5/97 1605
CBI stock cultures
11/1/97
20 g/kg
20.0-20.6C
No
N/A
C varieg a tu s Test 48-h Static Survival 11/3/97 1630 11/5/97 1615 Aquatic Bio Systems, Inc. 10/30/97 20 g/kg 20.0-20.6C No N/A -
fe rm io n necessary when effluent oxygen demand results in dissolved oxygen concentrations < 4.0 mg/1.
D-136
US00001317
O D I' OCE.\N.A Sample .D. Al I l 2.97 Sam ple97-123X3 Tesi IDr JRA97131
Coastal Bioanalysts Ine.
Sample Collection Date/Time
11/2/97 1830
. Sample Use Dates/Times
11/3/97 1635/16303
Aeration2 Rate/Time
n/a
Pretreatment/ Adjustment
HWM Salts
2Aeration often necessary to reduce sample oxygen concentration to 100% saturation after warming. 3_v/ bahhvC. variegatus test time.
Table 2. Effluent and Dilution Water 4 Chemical & Physical Characteristics (Mean Value (Standard Deviation))
Parameter
Temperature (C)
Salinity (g/kg)
Conductivity (uMHOS/cm)
pH'
Dissolved Oxygen (mg/1)
Total Hardness (mg/1 as CaC0 3 )
Alkalinity (mg/1 as CaC0 3)
Total Chlorine (mg/1)
n h 3-n (mg/1)
Initial Effluent
3.1
N/A
1624 (-- ) - 8.17 (-- ) ` 11.1 (-) 115 (-) 346 (-> <D.L. (-) 5.2 (-)
Adjusted Effluent 20.9 (...)
-2 1 (--) N/A
7.95 (-) 8.0 (-) fJ/A
N/A
N.D.
N.D.
Dilution W ater 20.7 (--) 20 (-) N/A
8.03 ' (--)
7.9 (-) N/A
N/A
N.D.
N.D. -
4EfHucnt parameters measured upon receipt of sample in lab and after adjustment on day of use. Dilution water (Wimex hw-Marinemix in ASTM Type 1deionized water) values measured after preparation of diluent on day of use.
D-137
US00001318
(jDt'.-OCEANA Sample I D. Al 11.2.97 Sample 97-12383 Test ID: JRA97131
II. W ATER Q U A LITY
Coastal Bioanalvsts Inc.
Parameter Temperature
(C) DO. (mg/1) pH
Salinity (g/kg)
Parameter Temperature
(C) DO. (mg/1) PH
Salinity (g/kg)
Canttfll
20.4 (0.3)
7.8 (0.1)
7.79 (0.22)
20 (0)
Table 3. Water Quality Summary
M. bahia Test
Mean Value (Standard Deviation)
12 ?%
25%
50%
20.3 (0.4)
20.3 (0.4)
20.3 (0.3)
20.4 (0.4)
7.8 (0.1)
7.7 (0.2)
7.7 (0.2)
7.8 (0.2) :
7.83 (0.17)
7.86 (0.13)
7.96 (0.04)
8.06 (0.07)
20 20 (0) (0)
20 21 (0) (0)
100%
20.5 (0.3)
7.8 (0.2)
8.12 (0.16)
21 (0)
Control
20.3 (0.3)
7.8 (0.1)
7.87 (0.16)
20 ()
C. variegatus Test
Mean Value (Standard Deviation)
6.3%
12.5%
25%
50%
20.4 (0.3)
20.4 (0.3)
20.5 ,(0.2)
20.5 (0.3)
7.8 (0.2)
7.7 (0.2)
7.8 (0.2)
m (0.3)
7.83 (0.18)
7.88 (0.11)
7.94 (0.05)
8.05 (0.06)
20 20 (0) (0)
20 21 (0) (0)
100%
20.5 (0.4)
7.7 (0.3)
8.08 (0.11)
21 (0)
D-138
US00001319
O D ! 'O C E A N A Sample I.D. A l
11. 2. 9 7 Sa m p le 9 7 - 1 2 3 X 3 T t I D : J R A 9 7 I 3 I
III. OUTFALL DATA SUMMARY
Coastal Bioanalysts Inc.
Test Date
9/30/97 11/3/97*
Table 4. Cumulative Data Summary - Acute & Chronic Tests (Tests conducted on this outfall by CBI)
Invertebrate
Acute .Tests
Chronic Tests
LC50 T.U.
NQEC T.U.
Vertebrate
Acute Tests
Chronic Tests
LC5 T.U.
NOEC T.U.
>100% <1.08
>100% <1.00
N/A N/A N/A N/A
>100% <1.00 >100% <1.00
N/A N/A N/A N/A
` Results submined with this report
IV. QUALITY ASSURANCE
M. bahia - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.): `
C variegatus - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
CBI Stock Cultures 10/15/97-10/17/97 KC1 (Sigma "Ultra" Lot#76H 10391) 343, 490, 700, 1000 mg/1
100% 574.2 mg/1 (540.9-609.5) 612.1 mg/1 (488.3-735.9)
Aquatic Bio Systems, Inc. (same batch as test) 11/3/97-11/5/97 KC1 (Sigma "Ultra" Lot#76H10391) 432, 720, 1200, 2000 mg/1 100% 1200.0 mg/1 (1070.5-1345.2)
1074.9 mg/1 (891.2-1258.6)
D-139
US00001320
CUMULATIVE DATA SUMMARY NPDES Permit #: N/A Oceana - ODU A-l
Date of, J TestV -V
2/12/97 >100
Vertebrate^.
-.-i Invertebrate
.... .Vertebrate.,.
S 3 H N M S & NOEe.0 r > Y N G E C ^ / ; 0 0
>100
2/28/97 Invalid
>100
3/12/97 >100
; ; >100
3/20/97 >100
>100
3/26/97 >100
>100
9/30/97 >100
>100
11/3/97 >100
>100
Supplement D-140
US00001321
COASTAL BIOANALYSTS. INC. NPDES STATIC ACUTT/SALTWATER
(Form AMB4S)
O ayl
% E ffluent
1.0.
t Liva
O ay 2 f Lrva
C o n tro l 6.25 I2.S
C-l 'G
C -2 _____ ! _ _______
M '0 _ J M1-2 ________
2-1 7 A 2-2 / )
IO (9 n
/b \0
3-1 l >
n25 3-2
4-1 IQ
50 4-2 <0 >5-1 f
100 5-2 O bM rvar
/t
G//
16
U
_ _ L ______
_______
I_______ 1 _ A l______
Tin;
7b G o
IJ..UZ
Final \ Survival
| O
(O
too
( O
{ oo
, oc)
M v s i d o p s i s b a h i a _____________________
Sourr
CBI
arch Oaio/TiTM:. f f / f K - i __________
cxIifTBtior w a l a r H W M A SW
O t h a c ___
S aJinry: S A
cdiiratumTarrp.(ranga): 7 O .In ________a 3 . 0 , [ j?__
*< jrg pnor to test:
XA n a n a a ^
________ / d O l h a r ___________________
r g during te s t: N ot fa d
A rtem a_ I X
/ d O t U r ____
300irlJ2
S O O irtQ
lO C O rrt
O th .r_
voliin: 200 H 3
JSOirt
500 ai Ot)r.
(H
bl orpm sm * n p f m t s : & _______ Ab-
------------
a n 4 1 6 1 : 8 0 f l O l i a r . ___________ R a m f a m a t o a n d u m . K ~ c l - 7
I / / ^ ? i ^ _JP : 0 * .
1
_ T u m W ata rA d M l l
T irm A nunal A(fcted_ n 3 r
___ S * t O p B y_
JM ENTATION USED:
A 1t m s m a i a r N u r r t a r
P.O . M a ta r 1 1 (2 8 0 1 4 4 7 S J I r i s a n & M atar (0 9 5 0 0 5 6 3 )
anus R i 60P B a l m {40020093}
SCT 3 3 M a ta r (H 8 0 1 6 7 9 2 )
gY S D .O . M a t a r f 2 ( 2 0 1 * 0 2 6 3 2 9 ) Comn} 2 4 0 p H M a t a r ( 9 C 2 6 8 )
S P P o rtap H 2 M atar (B S2360) f y S p an ati ATC R a h ac to m a ta r (A 366)
Day 0
Oay 1
Day 2
C i . 7-
3 0 ,-s
Q oO
T i i).7
E
2 ' S t o J?
o .a
-1V.&
M
p
3 a .*
3 ^ l o i 0 0 /
4 ,1 0 .1
5 7 0 .< 1
o.y
C U '3
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1 ao
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4
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In d ia li:
_ iL _
6 //
(//
NOTES;
Q uality A s u r a r a :
A s Q u * ty A iM M a officar (or this ta si I cartity th a t no pgnthcant f r v m n m from x x a p ta d p ro to co l o c c u n rd durw q rowtina p a c r m n i (w iIm s o t ^ a w a n o ta d h ak * * ) a n d th a t th a d a ta ic e u w a iy ra tla c t th a
c u t M it w m pa rfo n ro d b y CBI.
C enatiti
-
R ..J L . S W
D -m
TEST ID.; 1 f i t i
f.
US00001322
COASTAL BIOANALYSTS. INC. NPDES STATIC ACUTH7SALTWATER
(Form ACV4#)
% E ffluent
O ay 1
1.0 .
9 Lwa
D ay 2
9 1**
C o n tre!
6 .2 5 1 2 .5 25 50 [ to o
C -I C2 M 1-2 2-1 2-2 3-1 32 4-1 4-2 5! 5-2 O bserver Tim a:
1 (,
10
K
\o
16
/0 U>
1V
>
10
10
_________U t . fO
1L
I 0
/
1 lb
10 I
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C j -H-
A I5~i^ \ f _______
j
fin*! v S u rv iv v
\ f- j
CQ
I O tj \O d
-, C o 1C
Soeoes: Cyprinodon variegati
Source:
81
H atch O ate/T im e:_
A cchrm tion w a te r KW M A SW ^ 1
O t h e r ______________________S a l i n i t y ^ )
A c c lir m ti o n T s m c . [ra n g e ) : ^ U _______________t o
________________
Feeng pnor to t r
A rem ^
________ fd O t f r _
F eed n g d jn n g te st: N ot fad 0
A r ta m a ____________ fi
O thar__
C h an tier u i :
3 0 0 rri ( 3
5 0 0 irf
1 0 0 0 ni I Q
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Txt V otum : 2 0 0 rrt
250 ni
Initial no. o d a n o n e p a r m ^ ic a tr .
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3-
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R a rv fe n in o n te m p la te no.c v -- | ^
S E T U P : D *ta_ n / . V ? 7
_ TirrmWmt*Akd t
*1
T im e A /arrets A d tb d n * M
- _ S e t U p By C--( - ) _________
INSTRUM ENTATION USED:
A __S te m T im m n o te r N u r t a r
I
3 Y S D .O . M a te r 9 1 (S N E B 014 4 7 5 )
CP D ignans pH M it* r (D 9S 0Q 563)
Sino RISOPBilme 140020093} !D YSI SCI 33 Mwr (H8016792)
STS 0.0. Mar> 2 (Sft1*026329) Conn 240 pHJVbur (96268) SP PonipH2 Mit* (852360) ] Spmtm*TCR.fTMno<mlr(A366)
O thm
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fi, V A
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M 3
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*< ?3
T -.tfL
1 3 ? 2-. fit
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l.fs,
2
K.d
f - S 'l
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pH
3
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.7)
4
.OL
Y, Il
D .O . Im )
5
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C ?.?
1o
T.l
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5
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A
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S. 0
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At
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1 (^1
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A;
3/
Ar
3/
3 0r
h l _________ ___ h i t _________ O H
NOTES
TEST I D.: . i t $ -7 > 3 (
D-142
US00001323
COASTAL BIOANALYSTS INC. EFFLUENT/DELUTION W A T E R DATA (Form EFDLOI95)
I N I T I A L (ARRIVAL) EFFLUENT CHARACTERISTICS:
Sample Date l l l Q / 1 7 I
I
II I
Temp (C) I2.I I I I I I I
Conduct. (uMHOS) IKa^/ I I ' I I I I
Salinity (g/kg) | |
I
I
I
I
I
I
pH \ 8 a t - I
I
III
I IDiss. Oxygen (mg/1) I / / .
I
I
II I
Tot. Chlorine (mg/1) ILC>(_. I
.I
I
I
I
I
NH3-N (mg/1) I5, A I I I i I I
Hard (mg/1 CaC03) ln.<r !
I _!
I
I
I
IAlkln (mg/1 CaC03) I I Vi- I
I
Ii I
R.y i iCoior/Appearance1
I III
I I IInitials
O' If
I III
1 C-Clear O-Opaque Y-Yellow B-Brown Bl-Black G-Green T-Tuibid SI -Solids (S-Slighi M-Moderate V-Very Much)
FFFI UENT ADJUSTMENT/POST-ADJUSTMENT MEASUREMENTS:
ACUTE
Date [ Temp (C) | Salinity (g/kg) | D O (mg/l) | Aeration Rate | Aeration Time (min) | Adjust. D O. (mg/l) |
pH | Tot Chlorine (mg/l) |
Initials . |
| | | | | | | i | |
1 1 1 1 Bn/?/?
1!
11!
II4 M
1 ! 1 1 1 a\_
1 1 1 I
IIS. u
11
1 1 II
1 1 1 1 1 II --
1 1 1 1 1 il---
1 1 1 1 1 IIT-1T
1 1 1 1 HaO
1 1 1 1 1 Il VH
DILUTION WATER CHARACTERISTICS:
DMW:
H W M :.y
Date Temp. (C)
| |
| |
1 ! 11 1111
Conduct. (uMHOS) | | 1 1 1 1 1
Salinity (g/kg) | | 1
111
pH | I 1 1 1 1
Diss. Oxygen (mg/l) |
111 1 1
Hard (mg/l CaC03) |
1 1 1 1 "1
Alkln (mg/1 CaC03) | | 1 l 1 1 1
Initials
|
|
1111 1
ACUTE
Wll/Ai
IISt.TIII'./? II StA . 11.2, >3. Il Il /vi? Niv____ a i-/?
EFFLUENT ID: fl\
. T F S T ID
D-143
c t /T
7 -
US00001324
Toxicity Test Sample Chain of Custody (Please complete all information)
Facility. Q \ c p c m i / D O n Address K ) o 'j Xp r i *i 'K V"Ac
U m x i-p r Q itM
J
County '_____________________
Pipe/Outfall/Location _____________________ _
NPDES#______________________
Instream Waste Cone _______________________
Sample collected by fprint&signK Vjc^O y) -^ ^ S~6^ KV" Affiliation !}PU fcvW-bn T. L flb
Type of sample ^ /Grab):
Dace **/
Time ( o 'a O pVT)
~~ (Composite): From Date ~
Time
_______
.
To Date______ ~~ Time --______
Subsamples comprising composite:
Number____________ Frequency of collection_________ Volume__________
'Temperature of sample in sample collection device 0
Final temperature of effluent at sample collection point R 1- C/
'Is sample collection device chilled?
Is sample packed on ice for shipment?
'It is required that all samples remain at 0 - 4C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency (Do not freeze!)
Is the sample chlorinated?
dechlorinated?
If so, how?
____________
Permit with interim chlorine limit? ~~ If yes - limit (mg/L)______ ~ _____________
Field pH
Field Total Residual Chlorine____________
Comments/Sample description, A1 - tg U-terS { oonK af,
______
Type of test(s) to be performed
^,\or\rf) p - C cU G fi
(Specify organisms)
V/fVO & 0 A ~U-o_______________________
Method of shipment
(9CAA
Print & Sign Names
1. Relinquished bv i~V.
~ UAa JL Date
Time, \ G ' C C a r O
Received by_
---- -------- _ Date h / ? 1*7
Time, t o y s
2. Relinquished by ^
~__________ Date If / *\ Time M ^
Received bv r-3-~
_____ Date ^ f 3 f
Time m ?C>.
& * /--
/ z/ 3 / 9 7
FOR REED LAB USE ONLY
ir a V J 2 5 9 3
Arrival Temperature.
Co1or_______________ _____ Odor____
P H _________ ___
DO (mg/L)_
Conductivity (umhos/cm).
Salinity (ppt).
TRC (mg/L). _____ Method
On ice?
D-144
3v I t 1 0 / 9 *
US00001325
D-145
US00001326
CHAIIM-OF-CUSTODY RECORD
& ANALYSIS REQUEST FORM
PWC ENVIRONMENTAL LABO RATO RY
C O N T IN U A T IO N SHEET
SHEET S L
OF
US00001327
APPEN D IX D-3 BNR Inhibition Pass-Through Acute Toxicity Test Results 120 ppm A F F F P retreated w ith Best P erfo rm in g D efoam er
t
D-147
US00001328
D-148
US00001329
Coastal Bioanalysts Inc.
ODU/Oceana Acute Effluent Toxicity Tests
M ysidopsis baha & Cyprinodon variegatus
Sample I.D. Feed Stock Contract No. N00187-93-D-9748
s Delivery Order No. 0235 J O. No. 1912290, Item No. 0002BJ, 0002BH
PWC No. 1402 12/10/97 1000 Sample No. JRA97-14195
Submitted to: Ms. Merrill Anderson-Ashcraft
Navy Public Works Center Code 930, Building Z-140
Norfolk, VA 23511
I
By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062
(804) 642-0168
D-149
US00001330
O D I' OCEa NA Sample I.D. Feedstock 12 10/97 Sample 97-14195 Test ID: JRA97I75
Coastal Bioanalysts Inc.
I. TEST RESULTS
% Effluent Concentration
0
6.3
12.5
25.0
50.0
100
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acute Test Results
Mean (%) Organism Survival
M. bahia
24-h 48-h
C. variegatus
244i 4Sih
1Q0 100
100 ICO
100 100 100 100
100
' 100
100 100
95 90
100 100
25 0
100 25
00
00
33.0% 30.1-36.2 Spearman-Kaiber
3.03
42.0% 36.8-48.1 Speaiman-Karber
2.38
Acute Test Information:
Test Type:
End Point:
Test Start:
.
Test End:
Organism Source:
Hatch/harvest Date:
Acclimation Salinity:
Acclimation Temperature:
Test Aerated?1:
Aeration Initiation Date/Time:
M. bah ia Test
48-h Static
Survival
*
12/11/97 1610
12/13/97 1615
Chesapeake Cultures, Inc.
12/11/97
20 g/kg
19.1-21.0C
Yes
12/11 at 1640
C variegatus Test 48-h Static Survival 12/11/97 1600 12/13/97 1620 Aquatic Bio Systems, Inc. 12/3/97 20 g/kg 19.1-21.0C Yes 12/11 aX1640
'Aeration necessary when D.O. concentration <4.0 mg/1.
D-150
US00001331
ODV-OCEANA Sample I.D. Feed Stock 12/10/97 Sample 97-14195 Test ID: JRA97I75
Coastal Bioanalysts Inc.
Sample Collection Date/Time
12/10/97 1000
'
Sample Use Dates/Times
12/11/97 1610/16003
Aeration2 Rate/Time
n/a
Pretreatment/ Adjustment
HWM Salts
2Aeration often necessary to reduce sample oxygen concentration to 100% saturation after wanning. 3.V/. bahia/C. variegatus test time.
Table 2. Effluent and Dilution Water 4 Chemical & Physical Characteristics (Mean Value (Standard Deviation))
Parameter
Temperature (C)
Salinity
(g /kg )
.
Conductivity (uMHOS/cm)
pH
Dissolved Oxygen (mg/l)
Total Hardness (mg/l as CaCOj)
Alkalinity (mg/l as CaCO-)
Total Chlorine (mg/l)
n h 3-n (mg/l)
Initi>al Effluent 3.7 (-)
1 (...)
1280 (-)
6.86 (-)
9.5 (-)
,157 (-)
373 (-)
<D.L. < -)
29.0 < -)
Adjusted Effluent 20.9 (-) 21 (-) N/A
7.14 (-) 7.9 < -) . N/A B N/A
N.D.
N.D.
Dilution W ater 20.8 (-) 20 (-) N/A
8.08 (-) 7.9 (-) N/A
N/A
N.D.
N.D.
4EfTluent parameters measured upon receipt of sample in lab and after adjustment on day o f use. Dilution water (Wimex hw-Marineinix in ASTM Type I deionized water) values measured after preparation o f diluent on day of use.
D-151
US00001332
ODC-OCEANA Sample I.D. Feed Slock 12.-T0.97 Sample 97-14195 Tesi ID: JRA 97I75
0. WATER QUALITY
Coastal Bioanalvsts Inc.
(
I
Parameter
Temperature <C)
DO. (mg/1)
pH
Salini tv (g/kg)
'
Control
20.2 (0.7)
7.8 (0.1)
8.03 (0.09)
20 (0)
Table 3 Water Quality Summary
M. baha Test
Mean Value (Standard Deviation)
63%
20.1 (0.7)
7.7 (0.1)
8.02 (0.14)
20 (0)
113%
20.1 (0.9)
7.6 (0.2)
8.02 (0.16)
20 (0)
25%
20.0 (0.7)
7.4 (0.2)
7.97 (0.38)
20 (0)
50%
20.1 (0.8)
7.2 (0.8)
7.99 (0.65)
21 (0)
100%
20.0 (ID
6.8 (1.3)
7.80 (0.93)
21 (0)
Parameter
Temperature (C)
D.O. (mg/1)
pH
Salinity (g/kg)
Control
20.1 (0.8)
7.8 (0-1)
8.07 (0.04)
20 (0)
C. variegatus Test
Mean Value (Standard Deviation)
63%
20.1 (0.8)
7.7 (0.1)
8.08 (0.07)
11 3 %
20.2 (0.8)
7.6 (0.2)
7.97 (0.12)
23%
20.2 0.6)
7.5 (0.3)
7.95 (0.35)
50%
20.2 (0.7)
7.2 (0.8)
8.03 (0.47)
20 20 .20 21 (0) (0) (0) (0)
iaa%
20.2 (0.9)
6.8 : (1.4)
7.59 (0.64)
21 (0)
D-152
US00001333
O D I'O C E A N A Sample I.D. Feed Stock 12.10.97 Sample 97-14195 Test ID: JRAS7I75
HI. OUTFALL DATA SUMMARY
Coastal Bioanalysts Inc.
Test Pate
9/30/97 11/3/97 11/25/97 12/2/97 12/11/97*
Table 4. Cumulative Data Summary - Acute & Chronic Tests (Tests conducted on this outfall by CBI)
Invertebrate
Acute Tests
Chronic Tests
LC50 T.IJ.
NOF.C T.U.
V erteb rate
Acute Tests
Chronic Tests
LC50 T.U.
N O EC T.IJ.
8.1% 12.5% 9.7% 39.2% 33.0%
12.35 8.00 10.31 2.55 3.03
N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
3.5% 19.7% 13.8% 30.6% 42.0%
28.57 5.08 7.25 3.27 2.37
N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
Results submitted with this report
IV. QUALITY ASSURANCE
M. bah ia - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
C variegatu s - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
Chesapeake Cultures, Inc. (same batch as test) 12/11/97-12/13/97 KC1 (Sigma "Ultra" Lot#76H10391) 343, 490, 700, 1000 mg/1 100% 596.2 mg/1 (575.8-617:3) 608.1 mg/1 (491.4-724.9)
Aquatic Bio Systems, Inc. (same batch as test)
12/11/97-12/13/97
KC1 (Sigma "Ultra" Lot#76H10391)
432, 720, 1200, 2000 mg/1
100%
1231.0 mg/1 (1098.8-1379.2)
1077.9 mg/1 (893.7-1262.1) "
D-153
US00001334
CUMULATIVE DATA SUMMARY
NPDES Permit #: N/A
%,'r . - t . O e ^ n a j-O p U
; Feed S.
Date of . Invertebrate^- >' , . Test- -V
2/12/97 <6.25 2/28/97 Invalid 3/12/97 17.7 3/20/97 19.5 3/26/97 33 9/30/97 8.1 11/3/97 12.5 11/18/97 23.3 11/25/97 9.7 12/2/97 39.2 12/11/97 33.0
Vertebrate
31 35 52 35 34 3.5 19.7 36.6 13.8 30.6 42.0
y Invertebrate.--,,-" _ Vertebrate - .
NEC %"
1 n e c :%' '
.
Supplement D-154
US00001335
C O A S TA L B IO A N A L Y S T S . IN C . N P D E S S T A T IC A C U T D S A L T W A T E R
(Form S W A S T A T )
* l!lunt
1.0 .
0*yl I In *
C o n tro l tf U .r
re ' fi
C-l t r '
C-2 / n
1-1 ; fi
1-2 1 0
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2-2 i b
3-1 / e
3 - 2 1 i? 4-1 i b
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(m grt)
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Accbmaiion T*mp. Imroifc I * t
^ ^^
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F a a d r g ( n o r u> i m i :
A nTM
7 _____la 00wr-------- . ---------------------------------------------
(kg)
4
5
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7 1
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F u d ra j im i * t .r . N ot td
A r t u r ------------------1i
0 t t * : -----------------------------------
Unie
VP) _
0*t I J- f.3
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J L il_______ _ 3 0 -------
7/ - J l --
J j. _________
7(
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(r/>
r-/>
e * , *
300 *
500*13
1000*
O A - r .---------------------- *
NOTES:
Tut td o s : 3 0 0 *
250* $
500*
O t t - r ------------------------`
I n l ta . a TM p u r a d o n :
I ---------------**" " * " * * - ` 0 ----------------------
F to io p r o d 1 6 l:B 0
O t t a r . ___________ R a n * *
F?-
S E T U P : 0 . . . / A / / 7 / 4 . T : ______ -----------------------------------------------------------------------------
T . r a - A ra r m i. A d d a d l ( ? f t ----------------- S * U > B f ^ \ ^ >/ ' Q -
IN S W M W T A T IO N U S D :
S lam T la f i a t r a u r hrtr. A. \
-
Y S l .M .w # 1 (S N E M U W
g cp(>i~. *-**
S u ttfw R160P Salane (A0CC0093)
YS 1 3 3 M a w (H80IS792)
_ 0 Y 3 O .O .* J I5 W A fflM 2 9 )
*n***#"r'*0+
S P IW C M .l r(i5 2 3 M |
? S p * A T C M < ie t u r (661
O lh tr
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ib ( 4 , v - J ----------
/ft O f, 5 V 7 > /f l
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^
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A* hudiry Auoran^a o il* ' loi iN f tn^l I c j r t 't y th * t to n y , e 4 n l vq fDuiim inspactiom
Ou .wWm I`mm accp..p...t.a*d* pm *' " ^
m V a * * pulorrd W 0*1-
'
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_____ 1
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T E S T ID .:
+ . ,
D -1 5 5
US00001336
Toxicity Test Sample Chain of Custody (Please complete all information)
Facility_ Q \ d P b n i c n f o t v
A. ddr_e_ss_. VpVo rrApo\ttv. _T\]fAA n a1StTnD>ZO
J
County_ NPDES#.
Pipe/Outfall/Location. Instream Waste Cone
Sample'collected by (print&sign) v3Jo{en
--
Type of sample ^ fGrab):
Date Vl`22 -~ // \lXO3 //Q*Ti7T
Affiliation C P U ^fW
Time.t \ - _ _
X\ O QQ ij ril\
la b
.(Composite): From Date.
Time.
To Date__
Time
Subsamples comprising composite:
Number..______- - Frequency of collection_________ Volume_______
`Temperature of sample in sample collection device O ' ^
Final temperature of effluent at sample collection point
Is sample collection device chilled?
Is sample packed on ice for shipment? e s
'It is required that all samples remain at 0 - 4C during collection period and shipment for
data to be.accepted by the appropriate Regulatory Agency (Do not freeze!)
Is the sample chlorinated?
dechlorinated? Jiv If so, how? r - '
Permit with interim chlorine limit?_______ If yes - limit (mg/L)_
Field pH
?>
Field Total Residual Chlorine
Comments/Sample description. f a t e d 9 f \ v d i ^
Type of test(s) to be performed. MijS i d
C tjjT rtn cd irt V a r i e ^ a i v S
(Specify organisms) ____________________________________________
Method of shipment. P e U i t r y b u O D C i
Print & Sign Names
1. Relinquished by t J & fl/w jh r i 10S(?f1
Date l 2 - A H / ^ ? Time ^
Received by
Date/^ J/C ^^ "j? Time > f / >
Relinquished by
Date ' Time / /
Received
Date a - U - r 7 ____ 7
Time H&V
JRA#_ Color.
p H ---------
Salinity (ppt).
FOR REED LAB USE ONLY
Arrival Temperature ______
_____ Odor ____________
DO (mg/L)_
Conductivity (pmhos/cm)_
TRC (mg/L). _____ Method_________
On ice?. Solids"___ _ @_____ C
I
\m
I VM
> "I. )
D-156
Sev 5 /1 6 /9 4
US00001337
Print & Sign Names Relinquished by jU c ^ /'a / ) /7 a J '7 'Z ^ Date - / / / / ^ ~) Time /-? jt < Received by ( f W ^ Z>(<^> <o______ Date i q / i / f ? 7 Time f ? 3T Relinquished by________ _____________ Date____________ Time____ Received by________________________ Date____________ tim e____
D-157
US00001338
8 0 ` d IW iO i
Address repon to:
Merrill Anderson-Ashcraft Navy Public Works Center Code 930, Bldg. Z-140 Norfolk, VA 23511
Report Due Date: Sample Date: Sample Location: Contract No.: ' Delivery Order: J.O. #:
Ite m N o .: N P D E S P e rm it # : P W C #:
Sample ID: JRA ID:
----------------
i s i n m 7 - o ? - n - Q 7 4 8 _________________________
_______________________
_____________
n o c^i
; 'o o c Q
HO
2
\
SC1- J RRt?T T i' .R
D-158
oar
2 f : 9 t 6 6 T - S r - 03
US00001339
Coastal Bioanalysts Inc.
ODU/Oceana A cute Effluent Toxicity Tests
M y sid o p sis b a h ia & C yprinodon va rieg a tu s
Sample I.D. Mother Liquor Contract No. N00187-93-D-9748
Delivery Order No. 0235 J.O. No. 1912290, Item No. 0002BJ, 0002BH
PWCNo. 1401 12/10/97 1945 Sample No. JRA97-14194
Submitted to:
Ms. Merrill Anderson-Ashcraft Navy Public Works Center Code 930, Building Z-140 Norfolk, VA23511
E
By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062
(804) 642-0168
D-159
US00001340
O D I' OC KANA Sitmplc .D .\M lior I.juor
12 IO 97 $n;iiplo 97-14 94 Test ID: JRA97J76
Coastal Bioanalysts Ine.
i. t e s t r e s u l t s
% Effluent Concentration
0 6.3 12.5 25 0 50.0 100
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acute Test Results
Mean (%) Organism Survival
M . bahia
2 i h 48-h 10Q 100 100 100 100 100 100 100 100 100 100 95
C. variegaius
2hSx iSih
100 100 100 100 100 100 100 100 100 100 100 100
> 100% N/A N/A < 1.00
> 100% N/A N/A <1.00
Acute Test Information:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
M b a h ia Test
48-h Static
Survival
*
12/11/97 1620
12/13/97 1630
Chesapeake Cultures, Inc.
12/11/97
20 g/kg
19.1-21.0C
No
N/A
C v a r ie g a lu s Test 48-h Static Survival 12/11/97 1615 12/13/97 1615 Aquatic Bio Systems, Inc.
12/3/97 20 g/kg 19.1-21.0C No N/A
'Aeration necessary when D.O. concentration <4.0 mg/1.
t 1
D-160
US00001341
O D l'-O C E A N A Sam ple I.D. M other Liquor 1Z I0<97 Sample 97-14194 Test ID: JRA9717G
Coastal Bioanalysts Inc.
Sample Collection Date/Time
12/10/97 1945
Sample Use Dates/Times
12/11/97 1620/16153
Aeration2
Rate/Xime
n/a
Pretreatment/
Adjustment
HWM Salts
2Aeration often necessary to reduce sample oxygen concentration to 100% saturation after warming. 2_V/ bahia/C. variegatus test time.
Table 2. Effluent and Dilution Water 4 Chemical & Physical Characteristics (Mean Value (Standard Deviation))
Parameter
Temperature (C)
Salinity . (gAg) -
Conductivity (uMHOS/cm)
PH
Dissolved Oxygen (mg/1)
Total Hardness (mg/1 as CaCOj)
Alkalinity (mg/1 as CaCOj)
Total Chlorine (mg/1)
n h 3-n (mg/1)
Initial Effluent
4.0 (-)
1 (...)
1200 (~)
8.47 (-)
8.5 (-)
174 (-)
338 (-)
<D.L. (-)
1.8 (-)
Adjusted Effluent 20.7 (--) 20 (...) N/A
8.26 (-) 8.0 (-) N/A
N/A
N.D.
N.D.
Dilution W ater 20.7 (-) 20 (-- ) N/A
8.19 (-) 7.9 (-) N/A
N/A
N.D.
N.D.
4Efflucnt parameters measured upon receipt of sample in lab and after adjustment on day of use. Dilution water (Wimex hw-Marincmix in ASTM Type I deionized water) values measured after preparation of diluent on day of use.
D-161
US00001342
ODL'.'O CEA N A Sam ple I.D. M other Liquor 12/10/97 Sample 97-14194 Test ID: JR A 97176
n . WATER QUALITY
Coastal Bioanalysts Inc.
Parameter
" Control
Temperature (C)
20.2 (0.6)
D O. ' (mg/1)
7.8 (0.1)
pH 8.08 (0.12)
Salinity (g/kg)
20 (0)
Table 3. Water Quality Summary
M . baha Test
Mean Value (StandardDeviation)
J%
20.2 (0.6)
7.7 (0.2)
8.10 (0.12)
20 (0)
12.5%
20.2. (0.7)
() ** 3*2%
, U.l
..
lt
122%
.V
... 20.3 ' ' - T r 20.T.7
(0.6)
(0.7)
20J2 . (0.6)
7.7 (0.2)
7.7 (0.2)
7.6 (0.3)
7.7 (0.3)
8.17 (0.05)
8.24 (0.03)
8.31 (0.05)
8.37 (0.10)
20 20 20 20 (0) (0) (0) (0)
Parameter
Temperature (C)
DO. (mg/1)
PH
Salinity (g/kg)
Control
20.1 (0.7)
7.8 (0.1)
8.11 (0.10)
20 (0)
C. variegatus T est V
c M
t.
Mean Value (Standard Deviation)
r .
6.3%
12.5%
25%
50%
20.1 (0.7)
20.2 (0.7)
20.2 0-7)
20.3 (0.7)
7.8 (0.2)
7.8 (0.2)
7.7 (0.2)
7.7 (0.2)
8.13 (0.09)
8.16 (0.07)
8.23 (0.03)
8.30 (0.04)
20 20 (0) (0)
20 20 (0) (0)
100%
20.1 (0.7)
7.7 (0.3)
8.36 (0.09)
20 (0)
V
D-162
'i
US00001343
OIX'-'O CEA N A Sample l.D. M other Liquor I I'll). 97 Sample 97-14194 Test ID: JRA 97176
HI. OUTFALL DATA SUMMARY
/.
Coastal Bioanalysts Inc.
Test Date
9/30/97 1/3/97 11/25/97 12/2/97 12/11/97*
Table 4. Cumulative Data Summaiy - Acute & Chronic Tests (Tests conducted on this outfall by CBI)
Invertebrate
Acute Tests
Chronic Tests
LC50 T.IJ.
MQEC T.IJ.
Vertebrate '
Acute Tests
Chronic Tests
LC50 U L
NOEC T.IJ.
>100% >100% >100% >100% >100%
<1:00 <1.00 <1.00 <1.00 <1.00
N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
>100% >100% >100% >100% >100%
<1.00 <1.00 <1.00 <1.00 <1.00
N/A N/A N/A N/A N/A N/A N/A N/A N/A N/A
` Results submitted with this report
IV. QUALITY ASSURANCE
M . b a h ia - Acute
Reference Test Dates:
Reference Toxicant:
Test Concentrations:
Control Survival:
:
LC50 Reference test (95% C.L.):
Control Chart LC50 (95% C.L.):
C v a r i e g a t u s - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
Chesapeake Cultures, Inc. (same batch as test) 12/11/97-12/13/97 KC1 (Sigma "Ultra" Lot#76H10391) 343, 490, 700, 1000 mg/1 100% 596.2 mg/1 (575.8-617.3) 608.1 mg/1 (491.4-724.9)
Aquatic Bio Systems, Inc. (same batch as test) 12/11/97-12/13/97 KC1 (Sigma "Ultra" Lot#76H10391) 432, 720, 1200, 2000 mg/1 100% 1231.0 mg/1 (1098.8-1379.2) _ 1077.9 mg/1 (893.7-1262.1) "
D-163
US00001344
COASTAL 13IOAN ALYSTS. .NC. NPDES STATIC ACUTE/SALTWATER
(Form SWASTAT)
\ EMIumic Control
i. M
Day 1
1.0 . 1 I n *
Of 2 9 In*
C- 1 0 . i S L . ________
C-2 I f )
1o
1-1 i d 1-2 > 0 21 U )
f 0-- /o
l0
22 1 i ' . _____ L --
3-1 t o
1O
3-2 t o
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41 I I ) 4-2 I P
/p t-
5-1 ; b 5-2
nn. P i S
_____L O -- /o /O
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Tim:
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--
fin a l* Survival 10o
\ Co
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Sp*c*s:
Scare*:
l2 j-2 l3 -
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* * 1 * . TM -- HVMSW
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AcciimDltott Tr<i.
1 t --------------
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fWAngpoot to t u t :
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fa t in g Ajnnq tst: Not ftd
^ ___ l 0 l t * :
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300*
Tmvoium*. 200*
500^ 0
>000*
OAK.
2S0*$ 500"< "=.
tn b d no. cranurr par P t o o f ^ o i 16L:B0 5 O l i r ___________IW u ta m "
l '\ \
lieto
S T UP-. 0 ~ J 2 4 W 3 -- r * - ' " " -
T, ,, A rft*t
INSTRUMENTATION USED:
Stwn ThTTOiitr Nuir*r._4 U -
n Y3 0.0. Mtr # 1{S8014475)
CP D f u M pH Mtr (09500583) S-- R 160PB-- H O M 0 9 3 ,
Y S SET 33 M t.r (H8016792)
( 3 Y 3 D.Q. Mrtr 1 2 (9 0 1 * 0 2 6 3 2 9 ) C a n q 240 pH H . (9 6 2 6 8 )
S P P t^ ^ (6 S 2 3 S q _ 9 S p - W ATC M * " TM (A366)
DayO
Day 1
0r2
C T iO .i
T 1 ^ r) 4 2
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_.*!,) rn p ii------------- --------------'
Olhar
T E S T I P : T S fc d i . Ij- k -
D-164
US00001345
COASTAL BIOANALYSTS INC. E F F L U E N T / D IL U T IO N 'W A lc r c O A i A \rurm LriJLuiVi;
INITLAL (ARRIVAL) EFFLUENT CHARACTERISTICS:
Sample Date l/CW/o/'Wl
1
1 '.
i
i
i
Temp (C) I W. A 1 1 1 i i i
Conduct (uMHOS) l 0 t > 0 1 1 1 i i i
Salinity (g/kg) | | | I I l I I
pH I S . U 1
i
Diss. Oxygen (mg/1) s . r
iiii
Tot. Chlorine (mg/1) i i - O O 1 I 1 i i
i i i
NH3-N (mg/1) / . i f 1
1 .' 1
i
i
i
Hard (mg/1 CaC03) 1 7 - V I I 1 i i i
Alkln (mg/1 CaC03) 3 3 t r i
iiii i
Color/Appearance1 t y
i
i
i
i
i
i
Initials
< y&
i
i
i
i
i
i
t C-CIear O-Opaque Y-Yellow B-Brown Bl-Black G-Green T-Turbid SI -Solids (S-Slighi M-Modcrate V-Very Much)
FFFLUENT ADJUSTMENT/POST-ADJUSTMENT MEASUREMENTS:
ACUTE
Date | I I 1 I 1 1
Temp. (C) | I I 1 1 1 1 \ \ > *
Salinity (g/kg) | 1 1 1 1 1 1 Il
D O. (mg/1) | | I 1 1 1 1 Il
Aeration Rate | | | | 1 1 1 Il -
Aeration Time (min) | | 1 1 1 1 1 Il -
A djust. D O. (mg/I) | | 1 1 1 1 1 Il -
pH | | I I
1 1 1 Il S . 3 u
Tot Chlorine (mg/1) | | 1 1 1 1 1 Hji/C?___
Initials
1
1
1
1
1
1
1 l Cr
DILUTION WATER CHARACTERISTICS:
DMW:
H W M :/
ACUTE
Date |
|
|
|
1* 1
1
-
Temp. (C) | I I 1 1 1 1 l l f t . Z
Conduct. (uMHOS) | | | | | , 1 1 H/V'g ___
Salinity (g/kg) | | I I 1 1 1 Il a a
pH 1 1 1 1 1 1
Il S . 1 1
Diss. Oxygen (mg/1) | | | | 1 1 1 1U ? .?
Hard (mg/1 CaC03) |
|
|
|
|
1 - 1
ILtf .
AJldn (mg/1 CaC03) | | 1 1 1 1 1 I M ____
Initials
|
|
I\
1 1 1 110 fe
EFFLUENT ID: n\.>/P/,P
4/ / jr j
________ TEST FD:
D-165
''l? /
US00001346
Toxicity Test Sample Chain of Custody
(Please complete all information)
OldFacility,
JbU M :O A U r i v e r S ' r h j
Address. Ucrfciy- . VA 2?bl ^
.-y REED
VV?
County. NPDES#. Sample collected by (print&sign). Type of sample. ^ (Grab):
Date.
Pipe/Outfall/Location . Instream Waste Cone
Affiliation. OC__U_
Time-- */
L zJ? C7
___ (Composite): From Date.,_____ Time _______
To Date________ Subsamples comprising composite:
Time_______ *' ..
Number___________ Frequency of collection..________ Volume.
'Temperature of sample in sample collection device O ' Q j
Final temperature of effluent at sample, collection point. r . o ' <L>
Is sample collection device chilled?
Is sample packed on ice for shipment?
It is required that all samples remain at 0 - 4C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency (Do not freeze!) .
Is the sample chlorinated? ^
dechlorinated?
If so, how?______________ *
Permit with interim chlorine- limit?____________ If yes - limit (mg/L)_____________________
Field pH
Field Total Residual Chlorine______
Comments/Sample description.
Type of test(s) to be performed
R h C ltJ ) . Q j^ ^ X tn O d O r \ V d f je & q j u S
(Specify organisms)
^ ________ _________________________________
Method of shipment. QgXtVervf h j O Q U
Print & Sign Names
1. Relinquished bv
\ J o S O t 1 Date
Received by
Date l ? f ^ [ f J
2. Relinquished b y A r r u O k i,
Date /.-V/
Received by. -------
k tL J/U -J / ______ _ Date /2-u~<~7
Time
Time
( (\
Time J . v -
Time It <=>_______
r2(V 3,"
JRArf__________________ CoIor_______________ _
FOR REED LAB USE ONLY Arrival Temperature__________ _____ Odor _______________
pH_________ DO (mg/L)_______ Conductivity (pmhos/cm)_ Salinity (ppt)_______ TRC (mg/L)______ Method_________
On ice? . Solids. @ _1_
rf,}
\
D-166
Re 5/ 16/94
US00001347
Print & Sign N am es
Relinquished by.
Date / ? J / / / & J T i m c _ l 3 J - d
Received by P r V w - ^ \<yo C o _______ Date i ~ > . i u ( i n Time f 3 ~f
Relinquished by____________________ Date______________ Time_________
Received by___________________________ Date______________ Time_________
D-167
US00001348
D-168
US00001349
APPEN D IX D-4 BNR Inhibition Pass-Through Acute Toxicity Test Results
60 ppm A F F F P re tre a te d w ith F en to n 's R eag en t
S
D-169
US00001350
US00001351
ODU/Oceana Acute Effluent Toxicity Tests
M ysid o p sis baha & C yprinodon va rieg a tu s
Sample I.D. A1 Contract No. N00187-93-D-9748 ? Delivery Order No. 0235 J.O. No. 1912290, Item No. 0002BJ, 0002BH, 0003AD
PWCNo. 1371 11/17/97 2040 Sample No. JRA97-13121
. Submitted to: Ms. Merrill Anderson-Ashcraft
Navy Public Works Center Code 930, Building Z-140
Norfolk, VA 23511
. By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062
(804) 642-0168
D-171
US00001352
01)1'.OCEANA Sample I D. Al II 1 7 9 7 Sample 97-!J 121 T o l ID: JR A 97U 6
Coastal Bioanalysts Inc.
I. TEST RESULTS
% Effluent Concentration
0
6.3
12.5
25.0
50.0
100
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acute Test Results
Mean (%) Organism Survival
M . bahia
C variegatus
2M 4 M
24dh 48-h
100 100
100 100
100 95
100 100
100 100
100 100
100 100
100 100
100 95
100 100
100 100
100 95
>100% N/A N/A <1.00
>100% N/A N/A <1.00
Acute Test Information:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
M . b a h i a Test 48-h Static Survival 11/18/97 1730 , 11/20/97 1720 CBI stock cultures 11/18/97 20 g/kg 19.2-20.8C No N/A
C v a r i e g a t u s Test 48-h Static Survival 11/18/97 1730 11/20/97 1730 Aquatic Bio Systems, Inc. 11/15/97 20 g/kg 19.2-20.8C No N/A ^
V
D-172
US00001353
O D Ir <XTEa NA Sample ID . AI 11.17 97 Sample 97-13121 Test ID: JRA 97U 6
Coastal Bioanalysts Ine.
Sample Collection DateVTime
11/17/97 2040
Sample Use Dates/Times
11/18/97 1730/173 03
Aeration2 Rate/Time
n/a
Pretreatment/ Adjustment
HWM Salts
2Aeration often necessary to reduce sample oxygen concentration to 100% saturation after wanning. 3A/. bahia/C. variegates test time.
Table 2. Effluent and Dilution Water 4 Chemical & Physical Characteristics (Mean Value (Standard Deviation))
Parameter
Temperature (C)
Salinity (g/kg)
Conductivity (uMHOS/cm)
pH
Dissolved Oxygen (mg/1)
Total Hardness (mg/1 as CaCOj)
Alkalinity (mg/1 as CaC03)
Total Chlorine (mg/1)
n h 3-n (mg/1)
Initial Effluent
3.8 (--)
N/A
950 (-)
8.42 (-- )
11.4 (-)
145 (-)
258 (-)
<D.L.
(-- )
0.8 (-)
.
Adjusted Effluent
20.4 (--)
20 (-)
N/A
.
Dilution W ater
20.6 (-)
20 (...)
N/A
8.17 (--)
8.0 (-)
N/A S
N/A
8.05 (-) 7.9 (-) N/A
N/A
N.D.
N.D.
N.D.
N.D.
4Efflucnt parameters measured upon receipt of sample in lab and after adjustment on day of use. Dilution water (Wimex hw-Mariiicmix in ASTM Type I deionized water) values measured after preparation of diluent on day of use.
D-173
US00001354
O D t'O C E A N A Sample t.D. AI 11/17-97 Sample 97-13121 Test ID. JR.V97M6
"1
Coastal Bioanalysts Inc.
II. WATER QUALITY
Parameter Temperature
(C) DO. (mg/1) pH
Salinity (gAg)
Parameter Temperature
(C) DO. (mg/1) pH
Salinity (gAg)
Control 20.3 (0.3) 7.8 (0.1) 7.96 (0.14) 20 (0)
CODIXQl 20.3 (0.3) 7.9 (0.1) 7.89 (0.16) 20 (0)
Table 3 Water Quality Summary
M . baha Test
Mean Value (Standard Deviation)
63%
20.4 (0.3)
7.9 (0.1)
8.09 (0.11)
20 (0)
12 ?%
20.5 (0.3)
7.8 (0.1)
8.10 (0.10)
20 (0)
25%
20.3 - (0.5)
7.8 (0.1)
8.14 (0.07)
20 (0)
50%
20.3 (0.5)
7.8 (0.1)
8.20 (0.02)
20 (0)
C. variegaius Test
Mean Value (Standard Deviation)
6 j%
20.2 (0.5)
7.7 (0.2)
8.12 (0.11)
20 (0)
12.5%
20.3 (0.4)
7.8 (0.1)
8.13 (0.10)
20 (0)
25%
20.3 (0.4)
g
7.8 (0.1)
8.14 (0.08)
20 (0)
50%
20.2 (0.3)
7.8 (0.1)
8.18 (0.05)
20 (0)
--
100% 20.3 (0.4) 7.8 (0.1) 8.24 (0.06)
20 (0)
100% 20.2 (0.2) 7.8 (0.2) 8.19 (0.04)
20 (0)
D-174
US00001355
III. OUTFALL DATA SUMMARY
Test Date
9/30/97 1 1/3/97 11/18/97*
Table 4. Cumulative Data Summary - Acute & Chronic Tests (Tests conducted on this outfall by CBI)
Invertebrate
Acute Tests
Chronic Tests
LCM T.U.
M.QEC T.U.
Vertebrate
Acute Tests
Chronic Tests
LC50 T.U.
NOEC T.U.
>100% >100% >100%
<1.00 <1.00 <1.00
N/A N/A N/A N/A N/A N/A
>100% >100% >100%
<1.00 <1.00 <1.00
N/A N/A N/A N/A N/A N/A
Results submitted with this report
IV. QUALITY ASSURANCE
M . b a h i a - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
C v a r ie g a t u s - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.) Control Chart LC50 (95% C.L.):
CBI Stock Cultures
.
11/20/97-11/22/97
KCI (Sigma "Ultra" Lot#76H10391)
343, 490, 700, 1000 mg/1
100%
574.8 mg/1 (532.7-620.0)
610.6 mg/1 (488.4^-732.8)
Aquatic Bio Systems, Inc. (same batch as test) 11/18/97-11/20/97 KCI (Sigma "Ultra" Lot#76H10391) 432, 720, 1200, 2000 mg/l 100% 1029.5 mg/1 (881.7-1202.0) 1079.5 mg/1 (896.5-1262.5)
D-175
US00001356
|Datfcoi|
I 2/12/97 >100 | 2/28/97 Invalid | 3/12/97 >100 | 3/20/97 >100 | 3/26/97 >100 1 9/30/97 >1UU
11/3/971>100 11/18/97 >100
CUMULATIVE DATA SUMMARY NPDES Permit #: N/A Oceana - ODU A-l
Vertebrate LC50%':^1V^>100 >100 >100 >100 >100 >100 >100 >100
r x - Invertebrate^ n o e c %^St-
Vertebrate;.-- - -ir NG%~'
1
|" 1
Supplement
D-176
o US00001357
COASTAL BIOANALYSTS. INC. NPDES STATIC ACUTE/SALTWATER
(Form AMBA*)
%Effluent
D9 Isnyal DIoLrm2
C-l 1>
in
C-2 __Lfi____ ___ L_________ 1-1 A _ 3 ___________ 6.25 1-2 <b _ j __________
2-1 10 J A _ _ _ _
12.5 2-2 I _ L _________
3-1 IV 'O 25 3-2 U> l
l-l \b
lb
50 4-2 \ _ J ___________
5-1 10 .. _ ________
100 5-2 I
in
Observer: G-U
Cx|f
Time: n- it> I T 'r } fi_
FSwinvnarlaif 1 0o b |tO (O0
V?
100
Mvsidopsis bahia
Hitch Ota/Tim^ f m z m .
Acchrretion water HWM ASW
Other
Sour:
CBJ
Salinity:' l o
Acclimation Tempi Ira n ):
|
______ to _ "2 ^ X 1
fe e in g prior to rest: A n a m a . . 3 ~ k - J d O th e r
D jFooting dmng in t Not Ud
Attorno
/d O lh o r _
3C0irf R J
S O O iriD IOOOiW D Othoc:_
Test volume: 200 rrf 0
250/rt
500 n Q 0 t i w r ._ _
ImtuJ no. tvyarwm per n tfim i: f
No. nfUcMtm:
-
nupM el 16LB0 t Qthu: . Ranteretian tendete ng 'j> * '^ ^^
SETUP: Pea U ( ( X ( 4 .
Time Wotu Added f ? -
______
Tira Amtes Adtef_ / 7 - 2 > C >
S*U pBr
INSTRUMENTATION USED:
Stem ThermometerNuniw:_ 3 TS D.O. Motor 1 1(9S0I4475) 3 CP Dintorno pH Motor(09600563) -- Sonoma RIS0P su ora (40020)93)
YS SCI 33 Motor[HS016792)
T9 0.0. Motor 2 (901*076329) O m rq 2 P Motor(S7626S) SP PortopH2 Motor(8S23SO) ] Sportoti ATC Rofnctcmtor (A366)
O ther_______________ . ________ ___ _
C
T1
E2 M P3
4
S
C
1
2 3 4
5
C f
D.O. N T 1)
2 3 4
5
SC
AI L
2
N3 4
5 Replicale 9
Initials:
NOTE*
Day0 DayI Oey2
G ) d ,L a - 0 . V
Qp .l, '
S 1 .5 "
d U
D lu
d 7, 2
a 0 -H
V ?
Q lO .H
Z o -y . f i- r
Q . n ^ 0 .! J ,K J.K 1 1 ,1
y . a 5f 4/
y . l T . ?
6 .0 ? 6 . o J? ff./ y
C d S 44 fl $ 4 ./ f
S . >7 1 .4 '7- 4
T -.-f 7 -4 7 -4 r .o a ft o fr <1 /) jfl A0
7 .1
7 ..? 74
_ L J _______
4?
> 4-
4a
_______
7n
40
.
^ _________ 4 0
P<r
Ao
5 ,J Q q> Q
s l ________ A ________
ra l
B L _________ i i i Z --------
Qudtiy Auuance:
As Quality Assuwe officer for (hrs rast I certify that noserafica deviation from accepted protocol occurred ranng routine inspections (udess otNrwise rated below) and that the te a aecuatefy reflect the test ee it wee performedfy C8I.
Carments
-
Gl jl
to
SoM tM *
TEST I D.: ~ T ( l A 4 7 - /V(i> D-177
US00001358
COASTAL BIOANALYSTS. INC. NPDES STATIC A C U TESALTW a TER
(Form ACV4I)
* Effluent
1.0.
Oayl f Live
Q*r2 $ live
Control
6.25 12.5 25 50 IX
C-J I n
t0
C-2 I f A
!>
it 0
1-2 _____ L.________ __ LO_________
2-1 76
' Q _______
2-2 t o
i n _________
3*1 ( A
id
3-2 l
_ i __________
4-1 ( ( )
i
4-2 I ______! _________
5-1 I n ____ U l ________
j 5-2
Observer.
C >ff
Ture: I k o n
(1 3
final 1 Survival ( Oc (
l 0
t o 1 0o
^5
*r~-.**- Cvcrinodon vsrieqatus
Sourc.:
"EST / ) &
Accbrmtion water HWM ASW l _ l Other.
Suirvrv- 2 - 0
to l o . K
Feednj pnor to test; Arten j ) 'yC Id Other.
Peeng djnng test! Not fed D Arterie
Id Other:
O a rtu r .: 300rt Text volumi M O H
500*4 j lO X r i n
Other
2 5 0 * 4 .0
5 X r i t J Other
ri ^ ri ^
Initial no. oram ns oar replicale:____ 1 Q _____ No. rad este:
Photogvood 6L8 0
Oi>er.
ftandmiaetion torrefate na-l^- 1 j ^
SETUP: Dm. ( V / j % } ^ 1 T m W m iM M
Tana Ararmi* Adtfed ? - ^
s ubb* P O / i n *
INSTRUMENTATION USED:
Stem Themomrter Nuriwr. A t I Y9 0.0. Mmw * 1 (SNEB014475) 5 3 CP Diurno. pH M ttir (D9500S63)
Q Svtonus R160P Balance (40020093)
Y9 SCT 33 Mttr (H8016792)
E I Y S D.O. W a r 1 2 (SN91A026329)
L ] Coning 240 (H M .ur (96268) LJ SP PortapH2 Meter (852360)
3 *TC R.fncnxm ur (A356)
Other
OayO
Oayl
0ay2
T E M P
p
0.0.
Inerti
C 0) O (n
1 O d T" 2 S fO -ir
3
4
3 . V o <r
r d (? (r. Q oM
<P A . O , / f . S '. . / ir
5 ) /> .< -/ - c ) A ^
c gd<
1
2 f.J I
S ,iT
^ ) 7 .7 3 S . % .o D
3 87} 1
4 V 'lf
5 UT-
C . Vi
i 7 .5
2 7?
7-1
* .c r
7 -^ -7 0 : ........
3
T - .t
77
4
5
sc
A1 L2
N3 4
5 Radicate *
I n tie fs:
? ,
a o, 7n 5 a r/
t
(/1 f-
r> i i D ________ f ' , J 2 1 ________
7 L l 2 ________
3 /; ,9 r i
Q n i
..._ _________ . a r> 3 1)
0
0(4.
NOTES:
____ f
_______________________________________________________
1-- *~ -f<*r
t
- *-<*7_ --- 23----------------
Qtriiry Assurance: As Qiritry A u u v a officer for thrt tost I certify that no pthcant tfeyiarra frwn accepted protocol ocnm d during jouttno impactions (tries* otherwise notedbelow) and (hat the (feta accurately Meet the taet as ir was parfomed by CBI. Comments
o ~ k 3 r Cv) r Ft _ii / a i h i
TEST I D : < T L h 1 7 / 9 Ca D-178
/ i
US00001359
COASTAL BIOANALYSTS fNC. EFFLUENT/DELUTION WATER DATA (F o m E F D U )i9 5 )
INITIAL (ARRIVAL) EFFLUENT CHARACTERISTICS:
Sample Date f W//>/4? 1 1 1 ' - 1 1 1 Temp (C) 1 3 . 2 1 1 1 r i i Conduct. (uMHOS) 1 7 ^ 0 1 1 1 i i i
Salinity (g/kg) 1 M
1
1
i ii
PH i i 1 i i i
Diss. Oxygen (mg/I) 1 i / . V I 1 1 i i i
Tot. Chlorine (mg/I)
| 1i ii
NH3-N (mg/l) l o . t f 1 1 1 i i i .
Hard (mg/l CaC03) I f VS" 1 1 1 i i i
Alkln (mg/l CaC03) i s r s - ' i
i 1 iii'
Color/Appearance1 C. y
i
i
1i
ii
Initials
C-K 1 ' 1
1
i
i
i
1 C-Clear O-Opaque Y-Yellow B-Brown Bl-Black G-Green T-Tiubid SI -Solids (S-Slight M-Moderate V-Very Much)
FFFLU EN T A D JU STM EN T/PO ST-A D JU STM EN T M EASUREM ENTS:
Date 1 1 1
Temp. (C) 1 ^ 6 , 7 1 1 1 1
Salinity (g/kg) | ^ > 1 1 1 1
D O (mg/l) 1 f t , O 1
1
1
Aeration Rate | -- Aeration Time (min) j ---
| |
1 11 1 11
Adjust. D O. (mg/l) | | 1 1 1
pH 1 3 7 1 1 I 1
Tot Chlorine (mg/l) | /\/ O 1 1 1 1
Initials
| C- I
111
1 1 '1 '1 1 1 1 1 1 1
ACUTE
I II 1 II 1 II 1 II 1 II 1 II 1 II 1 II 1 II 1 II
DILUTION W A TER C H A R A C T ER IST IC S:
DMW:
Date
In/tXrt? 1
1
Temp. (C) iQiO.Ln 1 1 1
Conduct. (uMHOS) | ( \ r A 1 1 1
Salinity (g/kg) | ^ a 1 1 1
pH I f f . D S ' l Diss. Oxygen (mg/l) | 7_. f |
11 1
Hard (mg/I CaC03) | | 1 1
Alkln (mg/l CaC03) | f j p ) |
1
Initials
| Q- /( |
11
1' 1 11 11 11 11 11 11 I1 11
I 1 1 1 1 1 4 1 1
ACUTE
II II II II II II II II II
EFFLUENT ID: Q \ ______________________ TEST ID: 7/Tf*? ^ 7 / ^ ^
ti 3 / )
D-179
US00001360
r j -i9 -iS S 7 i j- i l
JRft
Toxicity Test Sample Chain of Custody ('Please c-o--mYpilvente. aull uiniifuormation)
757 873 1498 P. l?
Addrcss_b J O r p . 7| K
^
County
NPDES#
,,
Pipc/OutfaU/Locatioa -- iInnssttrrccaamm Waste CCoonncc
Sam ple c o lle a c d by ( p m . 4 s i s o )!i I 2 S O ]1^ i ^
Type o f s a m p le _ ^ --(Grab):
D ix_ % / e p
^
------- (Composite): From Date___ ~
To Date Subsamplcs comprising composite:
Time Time
Number-- ----
Frequency of collection.
Temperature of sample in sample collection device ^ 0 * 0 j
Volume
Hna! temperature o f effluent ar sample collection point
Is
.
sample colleaion
.,
device
Vch~UilUleCdQ7._
itcs sa--m---prl-e- pi--ac--k.e--d.on iic.se*>flo,uri sshuijppmmeenntt?.' MCS
u s reqm rrf t o t . 1 sam ples remain at 0 - 4 X during collection period end s h l ^ T i
dota b e occep ted b y l i e ap p rop riate R egulatory A g en cy (D o n o t fr e e z e ')
0 2Is tie Sim ple Chl r iiile d . _ r i f ____ dechlorim ted? b O If so, bow ? V o .
Femur witb.interim chlorine lim i? --------- _ [f ycs _ t a i [
-
Field rpfF--------- ---- Field Tiuortaarl KRecsidauail Chlorine________ _
Comments/Sample description Ari -
( 4 W 0&rCkM<QCl ^
Type of tesi(s) to be performed. M j S l d O inrrt p , C ^ n r ^ n A n f i
('Sp* ecifay or-ga-a-n--i-s-m---sJ) - w OAI t \ Methodf oi ff ship_m__e_nt, nPoetiktV/te^rj. ub.jt f/ilCr su. ^
Q, "~
----------------
Print & Sign Names
L. Relinquished by_ ' J t - n n i p Z . ' x/ e s o ^ Date n //? /< 3 7 Time \ ( A ' C / Q ^ O
"Re7ce.i.v..e.d....b.y.-.-.-..-.-...j Lx &n jfLt eu^S ^Dh * 'L_ t!ei^h 2DhatJe_ l<Tf im? he ~VfT S Time ffijS & \ J tL - I
R eceiv ed
r C~ r* ^ 7 ' ,, _-_-_-_--_-_--_-_-- DDataete kt k, 3W/ <f,_nS__l ___ Time y 7 3_jQ
^ - / a ; j-i JRA#
FOR REED LAB USE ONLY
Arrival Temperature__________
Color ________
-- _ _ Odor_________
PH______ DO (mg/L). ---- - Conductivity (pmhos/cm),
Salinity (ppt)_
TRC (mg/L)______ Method________
On ice? . Solids, @____
h m w ri,
D-180
id* /16/?
US00001361
u n M iiM -u r-v^u o i vju r n c ^ v jr iu
ENVIRONMENTAL
& ANALYSIS REQUEST FORM
PWC ENVIRONMENTAL LABORATORY CODE 9 3 0 BLOG Z - 140
9742 MARYLAND AVENUE
NORFOLK, VA 2 3 5 11 - 3 0 9 5
PH: (75 7 )4 4 5 -8 8 5 1 FAX: (7 5 7 )4 4 5 -8 8 5 2
TYPE C CRAB CP-COMPOSITE, FLOW CT - COMPOSITE. TIME
MATRIX L LIQUID SW SWIPE S SOLID GS - GAS SS - SEMI SOLID
'.v C i.v;-.\ONTA|fjER
: P - PLASTIC ^ B i BAG C;
' A--AAMMBR(glaji)
GL - GLSiS':> .CARTRIDGE
T - TEFLON '/ TL TEFLO LI^EO LID ;
V VOA '
H i HEXANE RNSED V
PRESERVATIVE
1 - COOL TO 4 C S-H CL, pH<2
2 - HN03, pH<2
-0.008% N2S203, 4C
3 - H2S04, pH<2 7 - FIELD FILTER
4 NaOH, pH>12 8 - NONE
ruiW Anouwo (D in ); COMMENTS:
(FOR RU SH TURNAROUND STA TE REASON BELOW)
R EG U LA TIO N APPLIED:
RCRA ( )
HRSD ( )
SDWA ( ) TS C A ( )
SAMPLING/COLLECTION CHARGE: POSSIBLE SAMPLE HAZARDS: COMMENTS:
1
CWA ( ) P H O TO ( )
CAA ( ) OTHER ( )
SAMPLE DISPOSAL: ( ^RETURN TO CLIENT ( )0ISPO5AL BY LAB
. CUSTOMER IS RESPONSIBLE FOR A U CHARGES NECESSARY FOR THE PROCESSINO AND ANALYSIS OF SAMPLEISI
ALL RUSH SAMPLES ARE SUBJECT TO SURCHARGE.
BTHTUHBEfli---------------------- INIT.:
CONTRACT LAB:
DATE:
CONTRACT NO.(S): OFF/CML USEONC?--- ------
2. S A M P L E S R E C E IV E D A F T E R 2 : 0 0 P M M O N . - T H U R S . A N D 1 :0 0 P M O N F R IO A Y W ILL B E P R O C E S S E D T H E N E X T B U S IN E S S O A Y (7 : 0 0 A M - 3 : 3 0 P M I.
* * TOR LA B U SE ONLYII
REJECTED( )
REASON:
HNtLINIJUISHEU BY:// ^ T T T
I!lHREET'-Tl'nIUQUUTrSSHHLtUU'BB YY';'V 7 ~,L\ 1
If P LT'INOUrSHEIT' i r f
, \\
IfPEnillJUISHLIJ B7
HOLDING TIME( )
' ' SAMPLES' VERIFICATION 'CONTAlfclERSf )
INITIAL:
J 7*7
" JR EC U 'H Y
W |H bC'U BY
|Hfc(j'U B Y
L U M P A N Y IC U M M A N U : / ) f A ) f
-- .......... U A T c / I M E: W f l Y . H n IO C
"'"T U M P N Y iO U M M A N U :
-- -L _
U A Ib / IIM b :,,^ ^ / ^
----
C M P A F IY IC T J M M A N U :--------------------------u x - i
U A , M M t: n /* R r u e .
U A Ifc/l IM t: f
7 . n i (/
D-182
US00001363
Coastal Bioanalysts Inc.
O D U /O ceana A cute Effluent Toxicity Tests
M ysid o p sis b a h ia & C yprinodon variegatus
Sample I.D. A3 Contract No. N00187-93-D-9748 * Delivery Order No. 0235 J.O. No. 1912290, Item No. 0002BJ, 0002BH, 0003AD
PWCNo. 1373 11/17/97 1855 Sample No. JRA97-13123
Submitted to: Ms. Merrill Anderson-Ashcraft
Navy Public Works Center Code 930, Building Z-140
Norfolk, VA 23511
By: Coastal Bioanalysts, Inc.
P.0 Box 626 Gloucester Point, VA 23062
(804) 642-0168
D-183
US00001364
O D I-O CEA N A Sample I D. A3 11/17,97 Sample 97-13123 Test ID: JRA97M 5
Coastal B io a n a ly s ts Inc.
I. TEST RESULTS
% Effluent Concentration
0
6.3
12.5
25.0
50.0
100'
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acute Test Results
Mean (%) Organism Survival
M . bahia
C. variegaius
24-h 48-h
24-h
48-h
100 95
100 100
100 95
100 100
100 100 100 100 100 95 100 95
100 100 100 100 100 100 ' 100 100
>100% N/A N/A <1.00
'
>100% N/A N/A <1.00
Acute Test Information:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
M . b a h i a Test 48-h Static Survival 11/18/97 1735 11/20/97 1700 CBI stock cultures 11/18/97 20 g/kg 19.2-20.8C No N/A
C v a r ie g a tu s Test 48-h Static Survival 11/18/97 1735 11/20/97 1710 Aquatic Bio Systems, Inc. 11/15/97 20 g/kg 19.2-20.8C No _ N/A
*1
D-184
US00001365
ODI.'-OCEANA Sample I.D. A3 11/17/97 Sample 97-13123 Test ID: JRA97145
Coastal B ioana lysts Inc.
Sample Collection Date/Timg
11/17/97 1855
Sample Use Dates/Times
11/18/97 1735/17353
AerationRate/Time
n/a
Pretreatment/ Adjustment
HWM Salts
2Aeration often necessary to reduce sample oxygen concentration to 100% saturation after warming. JM . bahia/C . variegatus test time.
Table 2. Effluent and Dilution W ater4 Chemical & Physical Characteristics (Mean Value (Standard Deviation))
Parameter
Temperature (C)
Salinity (g/kg)
Conductivity (uMHOS/cm)
pH
Dissolved Oxygen (mg/1)
Total Hardness (mg/1 as CaCOj)
Alkalinity (mg/1 as CaCOj)
Total Chlorine (mg/1)
n h 3-n (mg/1)
Initial Effluent
2.3 (-)
N/A
990 (-)
8.09 (--)
12.0 (-)
135 (-)
296 (-)
<D.L. (-)
6.4 (-)
Adjusted Effluent 20.6 (-) 20
(...)
N/A
8.01 (-) 7.9 (-) N/A
%
N/A
N.D.
N.D.
Dilution W ater 20.6 (-) 20 (-) N/A
8.05 (-) 7.9 (-) N/A
N/A
N.D.
. N.D.
4Efflucnt parameters measured upon receipt of sample in lab and after adjustment on day of use. Dilution water (Wimex hw-Marincmix in ASTM Type 1 deionized water) values measured after preparation of diluent on day of use.
D-185
US00001366
ODI.' OCEANA Sample I.D. A3 11/17.97 Sample 97-13123 Test ID: JRA97U3
II. W A TER Q U A LITY
Coastal Bioanalysts Inc.
Parameter Temperature
(C) DO. (mg/1) pH
Salinity (gAg)
Parameter Temperature
(C) DO. (mg/1) pH
Salinity (gAg)
Control
20.5 (0.3)
7.8 (0.1)
7.90 (0.14)
20 (0)
Table 3. Water Quality Summary
M . baha Test
Mean Value (Standard Deviation)
6 3%
LU%
25%
50%
20.5 20.5 (0.4) (0.4)
20.5 (0.4)
20.4 (0.6)
7.8 (0.1)
7.93 (0.11)
7.7 (0.2)
7.96 (0.08)
7.7 (0.2)
8.01 (0.03)
7.8 (0.1)
8.11 (0.09)
20 20 20 20 (0) . (0) (0) (0)
100%
20.4 (0.5)
7.7 (0.2)
8.16 (0.13)
20 (0)
Control
20.3 (0.5)
7.9 (0.1)
7.89 (0.16)
20 (0)
C. variegatus Test
Mean Value (Standard Deviation)
6.3%
12.5%
25%
50%
20.4 (0.6)
7.9 (0.1)
20.4 (0.6)
7.8 (0.1)
20.4 (g0.5)
7.8 (0.1)
20.3 (0.6)
7.8 (0.2)
7.91 (0.14)
7.95 (OH)
7.99 (0.06)
8.06 (005)
20 20 20 20 (0) (0) (0) (0)
100%
20.3 (0.4)
7.8 (0.1)
8.09 (0.08)
20 (0)
D-186
US00001367
O D I* -`O C E A N A S a m p le I .D . .A3 1 1 .17/97 Sam ple 97-13123 T e st ID : J R .A 9 7 U 5
ID. OUTFALL DATA SUMMARY
Coastal Bioanalvsts Inc.
Test Date
9/30/97 11/3/97 11/18/97*
Table 4. Cumulative Data Summary - Acute & Chronic Tests (Tests conducted on this outfall by CBI)
Invertebrate
Acute Tests
Chronic Tests
LC50 T.U.
NOEC T.U.
Vertebrate
Acute Tests
Chronic Tests
LC50 T.U.
NQEC T.U.
>100% <1.00 >100% <1.00 >100% <1.00
N/A N/A N/A N/A N/A N/A
>100% <1.00 >100% <1.00 >100% <1.00
N/A N/A N/A N/A N/A N/A
Results submined with this report
IV. QUALITY ASSURANCE
M . b a h ia - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
C v a r ie g a t u s - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
CBI Stock Cultures 11/20/97-11/22/97 KC1 (Sigma "Ultra" Lot#76H10391) 343, 490, 700, 1000 mg/1 100% 574.8 mg/1 (532.7-620.0) 610.6 mg/1 (488.4*732.8)
Aquatic Bio Systems, Inc. (same batch as test) 11/18/97-11/20/97 KC1 (Sigma "Ultra" Lot#76Hl0391) 432, 720, 1200, 2000 mg/1 100% 1029.5 mg/1 (881.7-1202.0) 1079.5 mg/1 (896.5-1262.5)
D-187
US00001368
CUMULATIVE DATA SUMMARY NPDES Permit #: N/A Oceana - ODU * A-3
Date of'J*. Invertebrate^a-^ T e stis
2/12/97 >100 2/28/97 Invalid 3/12/97 >100 3/20/97 >100 3/26/97 >100 9/30/97 >100 11/3/97 >100 11/18/97 >100
V e r t e b r a t e ^ . ~ .f? invertebrate ; . Vertebrate _y y.-v NOEC % 1 V - ' NOEc % - , r
>100
>100
>100 >100 >100 >100 >100 >100
SiffiDLemem
D-188
l US00001369
COASTAL BIOANALYSTS. INC. NPDES STATIC ACUTE/SALTW ATER
(Form AM B48)
\ EIHurnl
Dyl 1.0. SLva
Day 2 9 M
final i Survival
c-t
IJ2_________
f
C-2
U) .
( i > ______
"b
11 .......... L ------
' t>
6.25 1-2 / 0 4 t - _______
2-1 l b _____ _ L ________ 12.5 2-2 / A _ L O ________ 1 u o
3-1 L_____ 0
i o
25 32 1 A ___ L ________
4-1 1 P
_ 5 _________
50 4-2 _____ L _______ ___ L ________
5-1 .____L _______ < D
13
100 5-2 J ____ _______
Observer
c-y
-
( ________
Tina: / f o Q ^ i Z b
Source:
CBI
( L m d i ______
Acclirronon water, nwm
1 1 2-__ T y a Y
I d O tw .
fwer dunnq test: Not led O
Anerne / -V- /d Other
_ . . . . . . 300 iri 7 |
500 iri
1000 tri
3ther *
rrf
Tait wluma:
0 2 S 0 rrtO 500 tri tmr.
liatlal rc. onjanuta par lamicata:___ --------
No. implicates: 7 _
rrf
Ptotoanod 16L80
Othac__________ !UnAm lio tartriata no-^ ' A . 0
SETUP. n ,, . ( i V M 7 7Tltm A rirals A d ife d l 3 V
I 7-_t_5~---------
SrtU pBy
INSTRUMENTATION USEO:
P \_ _lStemThemrTMtar ffci*er_
r s 0.0. Malar ! (SNE8014475)
n rp rifu m a pH Malar 109500563)
a Safloms R! 60P Balana {40020093} TS SCT 33 Malar (H80167921
Y S 0.0. Malar 9 2 (SN91Q26329) " Coming- 240 & Maur (36268) SP Ptmapffi Malar (852360) 3 Spartan ATC BafractnmatarIA366)
Other
C
T
E2 M
3
4
5
C
1
2
I*
3
4
5 C 1
0.0.
(mgrt)
2
3
4
;s
Sc
Ai
L 2
N3
4
IsAa) 5
Replicale 9
0y0
Oeyl
0*y 2
S o .V s O P -i?
r? .-^
3 O. \ r . %.<r t f.A r
J> A . A Q ir A .'5 <0 Ci S -Ir Azi ? .r 7 .to ' 7 .1 h
3 o .|
1 ?)/) A
lt.9 fi,K 7-71 T ? H. 7 -$ <
t.h ) %.> 9.) 7-.1 7 .?
ff. 2 .1?
7- T-
7 . 1 $ ...
f .J V _
> - .
-
m
' * r I 7--1 > . L 7-k, D (Q A
O K
^ & L-
7-7-
& 2--
SU)
D ol P
3- C A rs
IL) _
6
> /.
.0 0
a0 \. {, d
_ L ____ J l ______
A i - 2 ________ C-a/ (pif
NOTES
Qiaiity Assurance:
As QuNovA s oI I k k lor tha test I certify that no * 9r*heart v a ttn from Mxepied pwtoesl oecumd a i l urtine pect (tfteu otherwise noted below) and that the *ta accurately reflect the
M t m it was perfornvd >r CBI.
fL -i^ L - T A, fcfw
/o, \ i n . D*
TESTm- -t R a QT-
D-1B9
US00001370
CO ASTA L BIOANALYSTS. INC. NPDES STATIC ACUTE/SALTW ATER
(Form ACV48)
\ gtfluant
1.0.
Dayt 9 Lrv*
0y 2 9 Lrva
final t Survival
Contra)
6.25 12.5 25 50 100
C-l l O . C-2 _ _ U i ________ 1-1 t o .. 1-2 (
1 O ' _______
16
I 10
2-1 1 A 2-2 t o 3-1 / A 3-2 I 4-1 1 6
(0
_ J J ________ /
_ t0
f
4-2 5-1 5-2 0bsrvr Tut:
1 0 - ____ L _________ m - 1 f) .
(0 /Cai <
_ J > _________
A k t _________
f7 l
l co 1 O (O |0 o l 0 100
Soura:
'CW
U i.A O .iJT ,n ti-
t V 1 C* 1
-----------------------
Arxl,nton >er HWMASW. t j 0 tt-r
! U * * r _ 2 = Q ----
1 ^ "5--
"2 _ o . &
!d Olhar
Faadr during tut; Not f.d Q
Art"*
/d Othar
C tantorm r 300 nd
SO O n dgl 1000 fri L J Othar
Tut voluma: 2 0 0 n d Q
250 nd H
500 fri H Otfw.
~y No. mriieataa:
fri v
y fri
AmTooanod 16U80 p^l 0 tirar
Rsndxrizatian tarntet nD-^" A
<rr HP flat. I V M K 1 .. r .W .u r A 4 b d _ 1 2 T ^ ----------
T m Attirali Adbd ^^ 3 ^ )
SrtUoBr P O / ( ? *
INSTRUMENTATION USED;
Stara Thamomataf Nun*er__^2-1-----(~l Y 3 0.0. Mtr* I (SNE6014475) 3 CP Disum a |H Malar (09500563) Sartori RI 60P W ane. (40020093) Q Y3 SOT 33 Malar (H80I6792)
Q Y S 0.0. Mutar 2 (SB1A026329) Coming240 pH Malar (S6268)
S ' PonapHZ Mat- (8S2360) p ] Spartan ATC Rafractorratar IA366)
Dry 0 ________ Oar I__________ 0t 2
C 0>t> U 3 A,(n T r v
T 1 .D.% -i i T,? / .7 -
M
2 *3o f t =i > 3 3. ()? r-? OAr\ M .5?
4 i2i/ * S o i -
..... -
s di'.fp 30.^ M .7
C Y . o r T-.Z . 2-.73
1 %.*<r -l^l 7.2?
pH 2 S' o Ll 3 fr,^3
7.?i -
4 ro i
?,A5-
5 f . d l 9 . Ito y - u ________
C 2.1
1
> .f ..
_
20 .0 .
2-y - 7.^ -
tirarti 3 3--<t
-
4 >.5 r .? _ P - 4 -
5 l-.A
*7-^
S C .0 o A1 L2 N 3 Art
4 ri v (7
Q (V-- _
A._______
^A <^________
9^0) 4
5
Rapitesi*9
rQ A 5 II
1
_^U ^________
________
3a . Ab .
P ____
latiaia: H -- W / _
NOTES'________ _ __________________________________ ____________ ___________
i I - - - <&
~g l
l-------------- --
Quality Assurancs:
AlOmlryAina tutoHIcarlorIIm 1camlY*10*9",ia*
^xsn fromaccaptadprolacnloccurrulOjnngmutin,rapectioni
(urto.otluvmanotar!Wo)*~1 th* >"'""'T tutuitw pufortmdbyCS1.
CofTVTWUl :
"
-V------------ Ip u ,
i'/pfhn
________ -
T E S T I P < j H . A ? r /-VT1
D-190
1
US00001371
COASTAL BIOANALYSTS INC. EFFLUENT/DELUTION WATER DATA (Form EFDLOI95)
INITIAL (ARRIVAL) EFFLUENT CHA RACTERISTICS:
Sample Date 1
I I - ! I !
Temp (C) I a s Conduct. (uMHOS) 1 4 1 b
I III! II I !I
Salinity (g/kg) I (
II ! ! I
pH I S - ?
!!
Diss. Oxygen (mg/1) \ I 3 . 0
! III!
Tot. Chlorine (mg/1) I L o c
II I I I
NH3-N (mg/1) I (eM
II I I l
Hard (mg/1 CaC03) I r s s r
II I I I
AJkJn (mg/1 CaC03) I Q t ip I I I I I I
Color/Appearance1 c y i ! I I I !
Initials
I <-y i
I
III!
1 C-Clear -Opaque Y-Yellow B-Brown BI-BIack G-Oreen T-Tuibid SI -Solids (S-Slight M-Moderaie V-Very Much)
'
FFFI.U EN T A D JIISTM EN T/PO ST-A D JU STM EN T M EA SUREM ENTS:
ACUTE
Date h ///m ? 1 1 1 1 1 1 1!
Temp (C) lo ) CMp 1
111 1
1 II
Salinity (g/kg) 1A o 1
I
11
1 II
D O (mg/l) 1 T . f 1 1 1 1 1 1 II
Aeration Rate 1 - 1 1 1 1 1 1 II
Aeration Time (min) 1 -
1
11
1
1
1
II
Adjust. D O. (mg/l) 1 -
1
1
1
1
1
1
II
pH 1 3 .D ) 1 1 1 1 1 1 II
Tot Chlorine (mg/l) 1w P 1 1 ! 1 1 1 II
Initials
1 1111 1
DILUTION W A TER CH A RA CTERISTICS:
DMW:
Date 1 1
Temp. (C) IS o A * I Conduct. (uMHOS) | <vf7 |
11
||
Salinity (g/kg) 1
1
1
1
pH IS. o s ' 1 1 1
Diss. Oxygen (mg/l) 17 - , 4 1 1 1
Hard (mg/1 CaC03)
| 11
Alkln (mg/l CaC03) l Mt l
11
Initials
1 <cH 1
1
HWM: ^
1 1 I 1 1 1
1
1
1
1
I
!
1 1
1 l
1 1 i 1 1 1
1
!
1
A CU II;
II II II II
II
11
II
EFFLUENT ID:
n
ri- 0 ?
TEST ID: 'T A ^ ^ 7 I 9 5~ D-191
US00001372
NOU-19-1997 13:12
J RO
Toxicity Test Sample Chain of Custody (Please complete all information)
Facility Q l P C v T li> )\O rT U n \
A ddress. K > C X f c f e V / V
t u j
J
757 S73 1490 P .1 8
County.
Pipc/Outfell/Location.
NPDES*.
Instream Waste Cone.
Sample collected by (princ&sisjtkiJ o ^ O n
Affiliation !)DiJ EVw.
Type of sample (Grab):
Date. / n / ^ T
Time. . 6 - . 5 5 p r y
(Composite): From Date.
Time.
rx L aU
To Date Subsamplcs comprising composite:
Time.
Number___________ Frequency of collection________ Volume^.
'Temperature of sample in sample collection device 0 Qy
Final temperature of efilucnc at sample collection point c?<?. 3(L/
'Is sample coliecdon device chilled?
Is sample packed on ice for shipment?.
'It is required chat all samples remain ac 0 - 4*C during collection period and shipment Car
data to be accepted by the appropriate Regulatory Agency (Do not freeze!)
[s the sam ple chlorinated?
dcchlorim ted?
If so, how?
Permit with interim chlorine limit?.
If yes - limit (mg/L).
Field pH ~7.C\%
Field Total Residual Chlorine__________
Comments/Sample description. k b -
a e r a tio n )
Type of test(s) to be performed.. Ml] ' b i d O i n t t i p . C r i j p r i 'n o ^ o r l
(Specify organisms)
\ZtjlC\
________________
Method of shipmenr
_
Print & Sien Names
1 . Relinquished bv
J O S Q \* D ate n / l g / 0 7
Tine. \ Q ' - Q C a r O
Received bv f t j t A j>a
j OJ>> Date L l f t i h l Time.
Relinquished bv
Date f f l f / - l Time ( f .
R e c ^ v e ^ by^_ -^ 3 -
Date n f n h y
Time t j l c >
t< f/? i? 7 e /2 ,o
Js V ^ l
FOR REED LAB USE ONLY
JRA# T7 - f v a 3
Arrival Temperarme _____
Color_________
Odor_________ ;_____
pH______ DO (mg/L).______ Conductivity (pmhos/cm)_
Salinity (ppt). ____ TRC (mg/L)______ Method________
On ice?. . Solids _
@ __________ c
D-192
a 5/ i /<
US00001373
US00001374
L/nMiiM-ur-^uo i u u r n c o u n u
U L I t m IN h U tT IV IM I IU IN
MR
-,^ U r ENVIRO NM ENTAL
& ANALYSIS REQUEST FORM
PWC ENVIRONMENTAL LABORATORY CODE 930 BLDG 2-140
9742 MARYLAND AVENUE NORFOLK, VA 2 3 5 1 1 - 3 0 9 5 PH: 1757)445-8851 FAX: (757)445-8852
C O M P A N Y /C O M M A N D : f > ,<>ft ^ ^
CONTACT: W f t \ Q L i r \<~~ D U O K IC * I I V -V T Z V _ C Y T P H O N E : l | ^ -- ? X q EXT:
SIG NATUR
%
p r i - W ' - U
P E R M IT NO.:
, CODE; FAX:
LAB USt-.ONLY
LINE ITEM * SAMPLE NO SAMPLE ID/IOCATION
SAHru ON UMM CAIE
At TtPE IIME
m i l t .....
a g e /3 v T .1 3 7 l
4 3 .7 .J-
,1 * 1 3
\ $ lf ,IS 7 S
! il( p
A - 1 O o /-vive. 1 A " <5- C o n ly - n l ,PV.~ P i i ^ p r A - w I
6 - a a ff i; Ap
SIAM STOP
ld o /p 1*
:Y )4 iJ
On
SIAM nop
HJAn
siAJir i V i S stop
-SIAM S0P
Y \T .n
StMI
\ Sloe
SIMl
iT lO I<tAO
, 'firem v i d i m a . SIAM
1 * 1 1 <HiY(=d L j c |t r \r
^ SIOP
iD f.C
SAHPLED IT
sor
HAIftU
L
PRESERVATIVE 1 \
Of SAHPUS AOHtAIHERS
ANALYSIS
FIELD READINGS pH KHPCIUtURE OTHLA
La. i
1 f \ * t .................... 9 7 - 3 W -I
------------------
1 9 7 -1 ilO >
PAUtAYAltOH VfJUFUDIT
Cy V l-
(
-----------\ ' m >
............ ...........
4
9 7 - / /.5 3 9?-
9 7 - 3 ia T
---- --------------- ---------------- ---ct 7 - V I 3L
Jr**'....... ............ 9 7 - (->> ) -7
~T
~
--
+1
_L
G GRAB C F. COMPOSITE, FLOW CT-COMPOSITE, TIME
L - LIQUID S . SOLID
S W -SW IP E
G S . GAS
SS - SEMI SOLID
P -P L A S T IC > ;V B iB A a:.': :; i:;;::.::^A.MBR(Bai) G L-GLASS - C i CARTRIDGE T TEFLON : ;; T L * TEFLON LjhjED LID j V-.VOA ' 1:11 .HEXAfjERINSED '
PRESERVATIVE
1-
COOL TO 4 C
S-H C L, pH<2
2 - HN03, pH<2
6 0.006% Na2S203, 4 C
3 H2S04, pH<2
7 - FIELD FILTER
4 NOH, pH>12
6 NONE
rviiHAROUHO (Dip): COMMENTS:
(FOR RUSH TURNAROUND STA TE REASON BELOW)
R EG U LA TIO N APPLIED:
RCRA ( )
HRSD ( )
SDWA ( )
TSCA ( )
CWA ( ) CAA ( )
PHOTO ( ) OTHER ( )
SAMPLING/COLLECTION CHARGE; POSSIBLE SAMPLE HAZARDS; COMMENTS;
$
SAMPLE DISPOSAL: ( )R E TU R N TO CLIENT ( )DISPOSAL BY LAB
SOUMBER:----------------- INIT.;
CONTRACT LAB:------------------------- D A T E : '
/. CUSTOMER IS RESPONSIBLE FOR ALL CHARGES NECESSARY FOR THE PROCESSING AND ANALYSIS OF SAMPLEISI. '
AU RUSH SAMPLES ARE SUBJECT TO SURCHARGE.
CONTRACT NO.(S)i
v m a x c u sE only
2. SAMPLES RECEIVED AFTER 2:00 PM MON, - THURS, AND 1:00 PM ONFRIDAY WILL BE PROCESSED THE NEXT BUSINESS DAY 17:00 AM - 3:30 PMI.
' FOR LAB USE ONLYU
SAMPLES VERlHeATjljN ^ ' ,
REJECTED ( )
R E A S O N ^ ^ H O L D IN G TIME* )
CONTAINERS* )
IMPROPERLY SAMPLED* ) OTHER* )
HbUNlJuibHb BY . / / * . / , ,
[PhUIIUUISHLU BY:' y j
.?
[HHIIlUUISHfcl) 14Y; " g _ , |TTraiNUUISHEU'UY; -------------
~ ^1
-
-
IK t G U Ur;
\H^ U ' ,, . ai V r 1 W , |H tU 'U U Y ; ; 5 7 _ JJn
UUMMANYIUUMMANU; / D / T T 7 " T u MPANVIUUMMANU: * * / i J l c
'' ""MHANYIUUMMANU:' '
LUMKANY/GUMMANU: til 2
INITIAL:
DATE:
IL ~ U A ib /iiM t: i i / 19) c?7 lOd
uAihYiiMb:/ ; i / / ^ 7 / / A ;i
' U A It/IIM t:
n tri
Ua i b /11Mb; f
IT lo i
D-194
US00001375
ODU/Oceana Acute Effluent Toxicity Tests
M ysidopsis bahia & Cyprinodon variegcitiis
Sample I.D. A2 Contract No. N00187-93-D-9748
$
Delivery Order No. 0235 J O. No. 1912290, Item No. 0002BJ, 0002BH, 0003AD
PWCNo. 1372 11/17/97 1830 Sample No. JRA97-13122
Submitted to: Ms. Merrill Anderson-Ashcraft
Navy Public Works Center Code 930, Building Z-140
Norfolk, VA 23511
By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062
(804) 642-0168
D-195
US00001376
O D l'-OCEANA Sample I.D. A2 n 17 97 Sample 97-13122 Test ID: JRA97147
Coastal Bioanalysts Inc.
I. TEST RESULTS
% Effluent Tnncentration
0 6.3 12.5 25.0 50.0 100
'
48-h LC50:
95% Confidence Limits:
Calculation Method:
Toxic Units (T.U.):
Table 1. Summary Acute Test Results
Mean (%) Organism Survival
M. bahia
C. variegatus
24-h 48-h
24-h 4 M
100 100
100 100
100 95
100 100
100 100
100 100
100 100
100 100
100 100 100 95
100 100 ' 100 100
>100% N/A N/A <1.00
>100% N/A N/A <1.00
Acute Test Information:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
M . bah ia Test
48-h Static
*
Survival
11/18/97 1800
11/20/97 1740
CBI stock cultures
11/18/97
20 g/kg
19.2-20.8C
No
N/A
C variegatus Test
48-h Static Survival 11/18/97 1800 11/20/97 1750 Aquatic Bio Systems, Inc.
11/15/97 20 g/kg 19.2-20.8C No N/A "
D-196
US00001377
()DL` O C E.\N A Sample I.D. A2 11,17.97 Sample 97-13 122 Test ID: JRA97147
Sample Collection Date/Time
11/17/97 1830
Sample Use Dates/Times
11/18/97 1800/18003
Aeration2 Rate/Time
n/a
Pretreatment/ Adjustment
HWM Salts
2Aeration often necessary to reduce sample oxygen concentration to 100% saturation after warming. 3_v/ bahia'C. variegatus test time.
Table 2. Effluent and Dilution Water 4 Chemical & Physical Characteristics (Mean Value (Standard Deviation))
Parameter
Temperature (C)
Salinity (g/kg)
Conductivity (uMHOS/cm)
pH
Dissolved Oxygen (mg/1)
Total Hardness (mg/1 as CaCOj)
Alkalinity (mg/1 as CaC03)
Total Chlorine (mg/1)
n h 3-n (mg/1)
Initial Effluent
3.2 (-)
N/A
980
(- )
8.17 (-)
11.6 (-- )
138 (-)
300 (-)
<D.L. (--) .
6.2 (-)
Adjusted Effluent 20.6 (-) 20 (-) N/A
8.01
(-- )
7.9 (-) N/A'
9
N/A '
N.D.
N.D.
Dilution W ater 20.8
( ...)
20 (--) N/A
8.21 (-) 7.9 (-) N/A
N/A
N.D.
N.D.
4EiTlucnt parameters measured upon receipt of sample in lab and after adjustment on day of use. Dilution water (Wimex hw-Mnrincmix in ASTM Type 1 deionized water) values measured after preparation of diluent on day of use.
D-197
US00001378
ODI,'-fJCE.VNA Sample I D. A2 11.17,97 Sample 97-13122 T ea ID: JRA97147
D. W ATER QUALITY
Coastal Bioanalysts Inc.
Parameter Temperature
(C) DO (mg/1) pH
Salinity (g/kg)
Parameter Temperature
(C) D.O. (mg/1) pH
Salinity (g/kg)
Cornial
20.5 (0.4)
7.8 (0.1)
8.07 (0.12)
20 (0)
Table 3. Water Quality Summary
M. b ah a Test
Mean Value (Standard Deviation)
20.6 (0.3)
12.5%
20.5 (0.5)
25%
20.4 (0.4)
50%
20.4 (0.3)
7.8 (0.1)
8.09 (0.10)
20 (0)
7.8 (0.1)
8.11 (0.08)
20 (0)
7.8 (0.1)
8.14 (0.02)
20 (0)
7.7 (0.2)
8.15 (0.06)
20 (0)
100%
20.4 (0.4)
7.8 (0.1)
8.14 (0.11)
20 (0)
Control
20.4 (0.6)
7.8 (0.1)
8.08 (0.13)
20 (0)
C. v a rieg atu s Test
Mean Value (Standard Deviation)
6 3%
12.5%
25%
50%
20.5 (0.5)
7.8 (0.1)
20.6 (0.4)
7.8 (0.1) -
20.4 (0.4) *
7.8 (0.1)
20.4 (0.4)
7.8 (0.1)
8.07 (0.11)
8.10 (0.10)
8.12 (0.04)
8.12 (0.04)
20 20 20 20 (0) (0) (0) (0)
-
100%
20.3 (0.4)
7.8 (0.1)
8.09 (0.07)
20 (0)
3
D-198
US00001379
O D l 'O C E .V X A Sa m p li l.D . A 2 U 17-97 Sam p le 97-13122 T e si ID : J R .A 9 7 4 7
HI. OUTFALL DATA SUMMARY
Coastal Bioanalysts Jnc
Test Date
9/30/97 11/3/97 11/18/97*
Table 4. Cumulative Data Summary - Acute & Chronic Tests (Tests conducted on this outfall by CBI)
Invertebrate
Acute Tests
Chronic Tests
L C 5 0 T.U.
N O E C T.U.
>100% >100% >100%
< i.to <1.00 <1.00
N/A N/A N/A N/A N/A N/A
V ertebrate
Acute Tests
Chronic Tests
L C 5 0 T.U.
N O E C T.U.
>100% <1.00 >100% <1.00 >100% <1.00
N /A N/A N/A N/A N/A N/A
` Results submined with this report
IV. QUALITY ASSURANCE
M. bahia - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
C variegatus - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C L): Control Chart LC50 (95% C.L.):
CBI Stock Cultures 11/20/97-11/22/97 KCI (Sigma "Ultra" Lot#76H10391) 343, 490, 700, 1000 mg/1
100%
574.8 mg/1 (532.7-620.0) 610.6 mg/1 (488.4-732.8)
Aquatic Bio Systems, Inc. (same batch as test)
11/18/97-11/20/97
KCI (Sigma "Ultra" Lot#76H10391)
432, 720, 1200, 2000 mg/1
100%
1029.5 mg/1 (881.7-1202.0)
1079.5 mg/1 (896.5-1262.5)
-
D-199
US00001380
CUMULATIVE DATA SUMMARY. NPDES Permit #: N/A Oceana - ODU A-2
|D^.6fs| InverteBfatf^&^^
I 2/12/97 >100 j 2/28/97 Invalid
s?--A ^ >100 >100
| 3/12/97 >100
>100
| 3/20/97 >100 3/26/97 >100
>10 |> ioo
y/3u/y/ >100
>100
ll/3/97[>100
[>100
11/18/971>100
>100
Invertebrate: ; ^V:t ^^b iat^'i '';-- ' '
n |) c :.%
noec%
1 | :------------ 1 j
j j
------------- -- ~~
-..
Supplement D-20
US00001381
COASTAL UIOANALYSTS. INC. NPDES STATIC ACLTE'SALTWa TER
(Form AMB48)
\ EMuant
1.0.
Day1 t live
0*y2 9Line
Control 6.25 12.5
C-l i o
ib
C-2 /a _ i f l ____
1-1 M
t
t-2 ___ IA____ _ J _____
2-1 1 n
/
2-2 l
3-1 t 25 3-2 f(y
4*1 10 50 42 1
5-1 ` 0 IX 5-2 t o
Observer G if
>d -- L___
16
f
H
CrhC
Tina: W a
Final\ Snervai (C
% too
100 iOo 15 v
Scactw: M vsid o p s is b a h
Hatch PTima:_/l / (
Acclirrotwn w a ter HWM ASW Q y
Oth*o_
CBI _Saliniy. 2_o
Acclimation Tfrp. (rang);______|
g^-.
2 ^ o fr~
Ftcdnqpnortot*iL` Anema X ) C It Qthar
Chantarsize 300ni P Tasivnluma: 200ni
500ni 1000ni Othar 250ni 500ni Q Other
ni ni
liMtialno.omamnsparraplou: /-=>__ Na.rateata: -2Photupanod161:80 fA Othar Ranrtoiiationtwrin no.
"
SETUP: Data
TimaWatarAdM l T-Vri
TirmAmrabAdded I Q L 'l
SalUnBr
INSTRUMENTATIONUSED:
StaniThtnimrowNutriate A t YSD.O.MalarI (SW8014475) CPOipsansapHMalar(09500563)
I SartoriusRI60PBala.(40020093) I Y3SCT33Matar(H8016792)
S YS0.0.Malar12ISB1A026329) Coming240fHMaw(36268)
SPPort*Malar(852360) 3 Sfart!ATCRafrctormtar(A366)
Other
PyO_________ Dar I
Pay 2
c . M A . o ). f
1 .A A A .S o J
2 J , I -M A .
3 J y -?
4 w o-1 5 Q o .G
tu '*
C 2, r i/ S. a J
1 Y'- i'fc
2 1? ,1 P
3
S './f
4
f l.rD
5
C ? .?
1 Vat
U
r^D .J n3 /) J / 4 ,^ / lf 3 b -0 O A M2
? .o >
?. W f.U r ./f ? .< 3 l 1 -? 1 -f
2 T -.f
3 M
1 ,3 f.
>
4
5
M
C ?
lif ?r>
J.-W 1 -f G a
eS >
2 A 3 r^ /)
/? r,
O il 1b
4
S
catf
tials:
?o
3 f> 1
C ftl
n AQ
_____ Crh
NOTES
n
TEST I.D.: ~T ftA'Y J- / V-2-
D-201
/
US00001382
CONSTAI. UIOANAJ.YSTS. INC. NPDES STATIC ACUTE/SALTWATER
(Form ACVHf)
%Effluent 1.0. D9 eLrive1
OI aUrv2e
Control C-l <0
lo
C-2 __LA___ i a
1-1 ib
!
6.25 1-2 i>.__ ___L___
2-1 f . __ l ___
126 2-2 1b
fo
3-1 10
l
25 3-2 10 ___U __
4-1 16
l
SO 4-2 10 __ L___
5-t to
fU
100 5-2 f A__ - L ___
Observer CM
Crii
Time:
FSiunravliv\al ! C0 ico l co
[oo iOo \0o
Cvpriftodon variegato*_____
Hatch DatWTum:_
Acclinstiofl waten HWMASW O Acclimation Tamp. (range):_
Othan
Salinity: ^ . O
'Z-o-h
Feeing pnor to tesr: Arterrsa 2 y
/ < Other.
Feeing dunng test: Not fed S 3 Arterrsa ______
Other.
Chamber sm: 300 mi
500 iH 0
1000 r f
Other
Test volume: 200 r i
250 r i B
s a irii
Other
X V'
Initial no. o i p n v n per n d m n :
IQ
Phoiopenod J6LBD
Other.
Rarafamcabon temdata m .
I Vf l /SET UP: to
'LL. T m W w A d d ri
T m Ammali AdcW_ f b
SetU eB y P O / ( r *
INSTRUMENTATION USED;
Stem Thermometer N u trier
1
O rs D.a Meter > I ISNEB014475)
5 3 CPO^tetee pH Meter (09500563)
Sem ite RI60P Beline. (40020033)
YS SCI 33 Meter (HSOIS792)
gTS D.O. Meter $2 ISN91A026329) Cuming 240 [At Meter (96268 )
SPf t * Meter (852360) fe r u ti ATC R efractor.ter (A366)
Other
DayO
Day 1
Day 2
C
.T E M P
1 2 J 4
S
C
1
2 (H
3
4
5
c
1
0 .0 .
2
ImTO
3 4
5
Sc
A1 L
2
N3
4
5 Resticele 9
In tu ii:
0 b ,? O n . *
SXO .ff a o .ff
P o-? A A .5 T
> - S .1
o .T
-- T/ C->
0,5 ^ 0 L
a 0 - ( T>
M .f If.f
t. A l % . o
Y .IS ~
<d . b H ?. fi
S '. > & S .o }
1. I
8. f u fT ./V
fr/A . S'. i .
? A ._
"M
X3
X? X?
M
1 -1
X ff
> - f r .... X*
.
-j
4 r>
cio
>
A^
a0
A
A <")
A z>_____
A>
aa
_ ls i_
A /5
3 ri ^
J P ... _ a _______
C M _____ A z i ______ C M ......
NOTES:_____________________________________ _____________
"
1^* lw
*|C>/` * y T ^ 4 r ? > j
Quality Assurance:
A* Q u^ity Assurance officer for ties tu t I certify that no s^rehcant deviations tmm accepted protocol occumsd Aung routine inspections {dess otherwise noted below} tod that the accurately fleet the taet as i! wes pmformed by C8t.
4 r4 i^ N ~ ? 1
jy
| X ? i (*77
TEST I D.: <7fc/H 4 ? . l Y
D-202
US00001383
COASTAL BIOANALYSTS INC.
EFFLUENT/DILUTION WATER DATA (Fom, e f d l o i9 3)
Alkln (mg/I CaC03) | ^ O 0 I
II
II
I
Color/Appearance I U
1
11
11
1
Initials
1 trH i : i
i ~T
1
1
C-CIear O-Opaque Y-Yellow B-Brown Bl-BIack G-Green T-Turbid SI -Solids (S-Slight M-Moderaie V-Veiy Much)------
EFFLUENT ADJUSTMENT/POST-ADJUSTMENT MEASUREMENTS:
Date 1
1 11
Temp. (G ) l a p . L l
1 1l
Salinity (g/kg) 1 1 1 1 f
D O (mg/1) l 7 - . i I I I
1
Aeration Rate 1 ~ 1 1 1 1
Aeration Time (min' 1 -
1
I
I
1
Adjust. D O. (mg/I) | | | I I
pH 1 1 1 I
Tot Chlorine (mg/1) U d 1 1 | 1
Initials
11
| II
DILUTION WATER CHARACTERISTICS:
Date
U i/x/rt
Temp. (C)
1
Conduct. (uMHOS) \ a
l
Salinity (g/kg) 3t> 1
i 1 l 1
pH
Diss. Oxygen (mg/1) | T . f Hard (mg/1 CaC03) | A/fl Alkln (mg/1 CaC03) | a/ A
1 1 I
1
1 1 1 1
Initials
1 C-H 1
|
DMW-
i 1 1 1 1 1 1 1 |
HWM-, ^
i* 1 i 1 1 1 | 1 |
1 1 1
'
|
I 1 | ^
i
1 i 1 1 1 | 1 |
1 1 1 1 1 1 |
1 1 1
1 1 1 1 1 1 4 1 1
ACUTE
II II II II ' II II (|
II II II
ACUTE
II II II II ir II II II II
EFFLUENT I D : _ ^ ____________________ TEST ID: J 7*-/? C| 7 /V7 ft/3 7<3 D-203
US00001384
loxiciry 1 C3t Sample Chain of Custody (Please complerc all information)
Facility_Q l A. |? Q v n i/l\ O n U n i \f ^ r Q jp J Address. t O o r - ^ V v : , VA- t *
County.
Pipe/Outfoll/Location.
NPDEStf.
Instrcam Waste Cone
Sample'collected by (print&sigrik
Type of sample ^ (Grab):
Date__ ^ \ l / V
Afiiliaton?PU grtV. Time (i- 3 0
"" (Composite): From Date___
Time
To Date______-- Time.
Subsamples comprising composite:
Mb
9
Number__________ Frequency of collection________ Volume_________
'Temperature of sample in sample collection device 0 *(L>
Final temperamre of effluent at sample collection point < 2 3 .0 * 0
`Is sample collection device chilled?
Is sample packed on ice for shipment? lj S
Tt is required that all samples remain at 0 - 4C during collection period and shipment for
data to t>e accepted by the appropriate Regulatory Agency (Do not freeze!)
Is the sample chlorinated? ^
dechlorinated?
if SOj how?
Permit with interim chlorine limit? ~ If ves - limit (rng/L)______
Field pH '7-2*^'
Field Total Residual Chlorine__________
Commems/Sample description. A 0 .- 0-O V lW l' f e Kr a&c LtCY))
Type of test(s) to be performed
CVirufl p . C ^U pfinO d.O fl
(Specify organisms) Method of shipment
V/<3jf X ^ OVK._____
`
^
1. Relinquished by
Print & Sten Names sjo sp /^ . Date
Time : \ G - G O * r t
Received bv
Date Il l t Ufa 7 Time
~^f~l 3*^-
2. Relinquished bv Recejyed bv = a zM
Date If'/n J q ZL.
, /it
Dare l//<?/37
////9 f9 7 ts .g jo
Time /jQtr)---------
Tim e // O
FOR REED LAB USE ONLY
JRA#^ 7 -/3 /A 3 l
Arrival Temperature_________ On ice?.
Color____ ;____________ Odor_______________
. Solids.
pH_________ DO fmz/L _
Conduciviry (pmhos/cm)_
Salinity (ppt)_______ TRC (mg/L)______ Method________
____
D-204
a* 5 /1 6 /9 *
US00001385
Coastal Bioanalysts Inc.
ODU/Oceana Acute Effluent Toxicity Tests
M ysidopsis baha & Cyprinodon variegatus
Sample I.D.B1 Contract No. N00187-93-D-9748 * Delivery Order No. 0235 J.O. No. 1912290, Item No. 0002BJ, 0002BH, 0003AD
PWCNo. 1374 11/17/97 2030 Sample No. JRA97-13124
Submitted to: Ms. Merrill Anderson-Ashcraft
Navy Public Works Center Code 930, Building Z-140
Norfolk, VA 23511
B
By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062
(804) 642-0168
D-205
US00001386
O D l'^ X 'EANA Sample !.D. Dl 1J/7-P7 Sample 97-13124 Test ID: JRA27148
Coastal Bioanalysts Inc.
L TEST RESULTS
% Effluent Concentration
0
6.3
12.5 25.0 50.0 100
.
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acute Test Results
Mean (%) Organism Survival
M. baha
C. variegatus
24=h i&h
2L
4M>
100 100
100 100
100 100
100 100
100 95
100 100
100 100
100 100
100 95
100 100
100 90
100 100
>100% N/A N/A <1.00
>100% N/A N/A <1.00
Acute Test Information:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
M. bah a Test 48-h Static Survival 11/18/97 1810 11/20/97 1800 CBI stock cultures 11/18/97 20 g/kg 19.2-20.8C No N/A
s
C variegatus Test 48-h Static Survival 11/18/97 1800 11/20/97 1810 Aquatic Bio Systems, Inc. 11/15/97 20 g/kg 19.2-20.8C No N/A
D-206
US00001387
ODUOCE.AXA Sample ID . B1 11/17/97 Sample 97-13124 T en ID: JRA9714X
Sample Collection Date/Iims
11/17/97 2030
Sample Use Dates/Times
11/18/97 1810/18003
Aeration2 Rate/Time
n/a
Pretreatment/ Adjustment
HWM Salts
2Aeration often necessary tqjreduce sample oxygen concentration to 100% saturation after wanning. 3M. bahia/C. variegatus teSt time.
R--
Table 2. Effluent and Dilution Water 4 Chemical & Physical Characteristics (Mean Value (Standard Deviation))
Parameter
Temperature (C)
Salinity . (g/kg)
Conductivity (uMHOS/cm)
pH
Dissolved Oxygen (mg/1)
Total Hardness (mg/1 as CaC03)
Alkalinity (mg/1 as CaC03)
Total Chlorine : (mg/1)
n h 3-n (mg/1)
Initial Effluent
3.4
(-- )
:2
(...)
2310
(-)
8.24
(-- )
11.5
(-)
141
(-)
243
(-)
<D.L.
(-)
2.6.
(-)
Adjusted Effluent
20.6
(-)
20
(-)
N/A
Dilution W ater
20.8
(-)
20
(-)
N/A
8.01
(-)
7.8
(-)
N/A
8.21
(-)
7.9
(-)
N/A
N/A N/A
N.D.
N.D.
N.D. .
N.D.
--'
4EfIhient parameters measured upon receipt of sample in lab and after adjustment on day of use. Dilution water (Wimex hw-Marinemix in ASTM Type I deionized water) values measured after preparation of diluent on day of use.
7 D-207
US00001388
ODI ' OCKANA Sample I.D. Bl II 17-97 Sample 97-13 12-1 Test ID: JRA9714X
Coastal Bioanalysts Inc.
II. WATER QUALITY
Parameter
Temperature (C)
DO. (mg/1)
pH
Salinity (g/lcg)
'
Control
20.4 (0 5)
7.8 (0.1)
8.07 (0.12)
20 (0)
Table 3. Water Quality Summary
M. baha Test
Mean Value (Standard Deviation)
63%
12.5%
25%
50%
20.4 (0.3)
20.5 (0.3)
20.5 (0.3)
20.4 (0.4)
7.7 7.7 7.7 7.5 (0.2) (0.2) (0.2) (0.3)
8.07 (0.11)
20 (0)
8.09 (0.09)
20 (0)
8.11 (0.04)
20 (0)
8.13 (0.05)
20 (0)
100%
20.5 (0-4)
7.5 (0.3)
3.13 (0.11)
20 (0)
Parameter
Temperature <C)
DO. (mg/1)
pH
Salinity (g/kg)
Control
20.4 (0.6)
7.7 (0.2)
8.08 (0.13)
20 (0)
C. variegatus Test
Mean Value (Standard Deviation)
6.3%
12.5%
25%
5%
20.4 (0.6)
20.4 (0.5)
20.4 (0.6)
20.3 (0.5)
7.7 (0.2)
8.06 (0.11)
20 (0)
7.8 (0.1)
809 (0.10)
20 (0)
7.7 (0.2)
8.09 (0.07)
20 (0)
7.6 (0.2)
8.09 (0.03)
20 (0)
100%
20.4 (0-4)
7.6 (0-2)
8.08 (0.07)
20 (0)
D-208
US00001389
O D I.`.OCEANA Sample !.D. DI
1 1 .1 7 .9 7 Sam ple 97-13124 T e si ID : J R A 9 7 U X
III. OUTFALL DATA SUMMARY
Coastal Bioanalysts Ine.
Je s t Paig
9/30/97 11/3/97 11/18/97*
Table 4. Cumulative Data Summaiy - Acute & Chronic Tests (Tests conducted on this outfall by CB1)
Invertebrate
Acute Tests
Chronic Tests
I.C50 T.TT.
NOF.C T.TT.
Vertebrate
Acute Tests
Chronic Tests
LC50 T il.
MQE-C T.TT.
72.0% 1.3$ 50.5% 1.98 >100% <1.00
N/A N/A N/A N/A N/A N/A
>100% >100% >100%
<1.00 <1.00 <1.00
N/A N/A N/A N/A N/A N/A
` Results submitted with this report
TV. QUALITY ASSURANCE
M. b ah ia - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
C variegatu s - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
CBI Stock Cultures 11/20/97-11/22/97 KC1 (Sigma ''Ultra" Lot#76H10391) 343, 490, 700, 1000 mg/1 100% 574.8 mg/1 (532.7-620.0) 610.6 mg/1 (488.4-732.8)
Aquatic Bio Systems, Inc. (same batch as test)
11/18/97-11/20/97
KC1 (Sigma "Ultra" Lot#76H10391)
432, 720, 1200, 2000 mg/1
100%
.
1029.5 mg/1 (881.7-1202.0)
1079.5 mg/1 (896.5-1262.5)
D-209
US00001390
CUMULATIVE DATA SUMMARY NPDES Permit #: N/A Oceana - ODU
B -I
D ateofgg
T e s|fS if
2/12/97 >100
>100
2/28/97 Invalid
>100
. 3/12/97 >100
>100
3/20/97 >100
>100
3/26/97 >100
>100
9/30/97 72
>iuo
11/3/97(50.5
>100
11/18/97 >100
. |> 100
EffyateBrafeiSi
n&rr
Vareef-t-esBrr-ate"^ > > ^ \a NOEC%'^> '
~ ] --------------- -------
"|
1
------- ""----------------- |
"
-------------------1 1
8
Supplement D-210
US00001391
COARTAI. BIOANAI.YSTS. INC. NPDES STATIC ACUTE/'SALTWa TER
(Fonn AM BAS)
\ Eflluam
1.0.
Day! t live
0ey2 $ Uva
Control
6.25 12.5 25 50 100
C-I > O
>v
C-2 / 0 1*1 m
<0
It
1-2 _ J J 1 _______ 21 i n
1b
l
2-2 l o
3*1 0
3-2 I
________ in
/)
4*1 f t 4-2 f n 1 \ 0
1
_ i_ Q ________ if )
5*2 Observer:
ft
H-
_ ________
Ture:
fin a l* Survival
|tc
(C c
<**>
,C 0
To
ScaciaK M vsidopsis bahia
Snm ;
CBI
Hatch DtefTirpe:_ Acciination water. HWM ASW Q
Other._____________ Salimcy: 2 j & _
Accliration Tempi (range):______| ^ f lL _______ 10
Feeng pnor to t n t : A rren a X ) c - _/d Other______________________
LJfeein g dring tesr Mol fed
Arten j V
j d Ottime.
O a n ta rw e : 300 H ^
500ni
lOODrd
0ih -T _________ n i
Test volwnc 20O irt 0
250 nd
500 rr* O tiw r_________ tri
Im tal no. orm snc per faticete : f ^
Ftotopenod 161:80
Q tW :
: No. tto ta s : ^ -- ____ Reorfacmation terriete noj ^ j y \ -
SETUP: n i. t ' I f ) r ( A
Tam W aiarAdfcd I 7 * V 5 " ____
T i A i n liA d M 1 ? f Q _______ SMUpBy. ^ I ( * K
NSTRUMENTAHONUSED:
item Themormter NuvAer_ U 3 T3 0.0. Malar # I (SW 80I4475) 3 CP Oipaansa pH Malar (09600S63) Swnm RI60P B aiar (4C0B0093)
J T 3 SCI 33 Matai (H6016792)
Q YS D.O. Malar # 2 (381*026329) [ J C anw j 240 J * Malar (36268 )
SP PonapH2 M alar (8S2360)
] Sparta*TCRa4iaclritar(*366)
thar ___________________________________________________________ _
Day 0
Day 1
Day 2
C 2u>J
D -V-. / 1 - f
T
1 <=) A X
0 *3
f
E M
2 ? C> 7 - - p u . U
a o .
P a P3
0 .9
4
(q
4 ,7>o U
ao?
/4 . 9
S < 3 (j c 2 n
A .
c KS( I R .ti
(&
M?
2 ft.I l
tf. ii
lf.0 1
pH
3 f. l i * 0
4 6 , 0 7 - f f '. f ?
% ,r
S Y .o
0 .0 .
(rngil)
s A l
c
1 X -1
2M
3 ?-S> 4 A -S S ?.*> c
1 <3r; 2 Sa
3 .* 7 .7 7- Ls ? - C-* 7 17i o )D
AS7 : ^ .... IS --
7? X -.7 <3 D ___
^ 1 2 _________
N 3 0 4
^0 3o . . A d ________
5 Repeat* 9
<Q f ) I
3-D
_a_
tniiuts:
Wf
(r^
NOTES
Q udrty Assurance As Q udiry A surw ee ofhear for tts test I ce rtify f t * 00 s ^ n fio n t
from accepted protocol occurred Aerng eutine rmppenons o itw vm e noted bekw ) and that the t acewately fleet the test as rt was performed by CBI. Ca w iK i
(p v w tk J ') * 5 ^ ( 6
TEST I D.: ~ T ( l b r1 i l / 9 V
D-211
/
US00001392
COASTAL UOAN ALYSTS. INC. NPDES STATIC ACUTE'SALTW ATER
(F orm ACV4)
\ EffluOt
1.0 .
Dr 1 f 1>V*
0 *y 2 $ Uv*
Control
6 12.5 25 50 100
C-1 C-2 11 1-2 2-1 2-2 3-1 3-2 4-1 4-2 5-1 5-2 Otsorvor.
1 f
fo tO /0 f 1b
> 10
1h /a
1A
c^y-
>>
_ i _ 2 _______ l 16
I ] _ J i > ________
'0
_____ i l ________ 10
_________
10 -- W _________ G 'f r
Titra:
M o -- I S I .....
fino! \ Survive
ic
tC 1 CO
|0 0 1c
A1 ______
Cvnnnndon varieqatus
S=-- :_____ ,
LJAccliminon water HWMASW
Other
SoJinnr 2 _ 0
Faodnpnortoto*c Anon
lo
.K
'yC. ,f Other
Foodrg doing tn Not fod E ) Ariamo
/d O liar.
C lw rfw w .: 3 0 0 ^
500irt 0
1000 n i Q Other
rH ^
Tw volum .: 200-rt
250irt g ]
500*4
(),[ ,.
In tie f no. oroanonm por radc o ir
1^
No. radicato:
^
PTvtoponod 16LB0
O ihor
RonAorim ioii a n fe n a l * f j
SFIUP-. D . f l f l ' i ) l r m W A d W f ? - Y 5 ~
Tina Annal Added 1
sm u o Bv P O fo r*
INSTRUMENTATION USED:
Sum Thenromator N u rta r A 1 TS 0.0. M * 11(9 *8 0 1 4 4 7 5 )
CP Or^oonM pH Motor (09500563) S viorits RI 60P Balano (40020093) YS SCT 33 M aw (H8016792)
5 T 9 D.O. M v ta rf 2 (91AQ26329) L - 1Coming 240 pH Motor (SN5269) SPP b rtu M .t*f (B523S0) f t } ^am A T C R *hiciim urlA 366)
Other
C 1 2 3
4
s
C
1
2 PH
3
O.D. {m gflj
4
5 C 1 2 3
4
5
SC A1 l I :2 H -3
4
5
$cato
tais:
NOTES:
0*r0
O arl
O t ?
a ,? 3i . 7 -
h u .7 ' .Z
3kO-Co
j i
? .n
z.n r
2 .0 } f
T -.-r
P If 30? Q Vt
A-S"
<2 6 . 1
S .o b
%, o i d . 9
* ,//
$./. K .rH l (u
2u
-i* ?
/4.a
/ .^ ?
f.o O S '.d l
7.1 2 .1 -
7 -fc
T -.i
*?
? ,
....
7-H % &-C>
>*7
-$ 6 ... 2) O
^0 ^ t>
r- i 0
A o?
T 3
. 1 0 ... l
0 4 A ... I P -- ./ _ i _______.:
S d L - _ L 2 ______ f - H
Oualtry kssmncm:
'
As Quabry A su rm c* o ffic e r fo r thr* la st t c o rtify lh * t no s^w heant fe vta tn rs from aecoptod protocol occurrad fenn m rtino ipoeiJO ( t f a othorwrso noto* bolo) and iht th* fe u a c m tM y m floe t tho toot s it wos fe rfo rrrod by CB.
Corn ionts
O f f n e n - , f f i f e Q r
TEST I.D.:
9J fY f
D-212
US00001393
COASTAL BIOANALYSTS INC.
EFFLUENT/DDLUTION WATER DATA <Fom.EKDLOi95)
IN ITIA L (ARRIVAL) EFFLU EN T CHA RACTERISTICS:
Sample Date
Temp. (C) \ 2 . H ' I
1
j 1
i
1
1 1
1
Conduct. (uMHOS) |Q 3iA I I I I I !
Salinity (g/kg) \ n
i
11
pH
lo y
i
i
11
1
Diss. Oxygen (mg/1) 1 / 1 . S I
11
1
Tot. Chlorine (mg/1)
111 11 1
NH3-N (mg/1) IX).4> 1
1
11
Hard (mg/1 CaC03) 1 /w__ !_____ !__ 1 1 1 1
IAJkJn(mg/lCaC03) |Q < /3 I
1
11 1
Color/Appearance1 1 C r 1 . 1 1 1 1 1
Initials
1
1
1
1
1
1 C-CIear O-Opaque Y-Yellow B-Brown BI-Black G-Green T-Turbid SI -Solids (S-SIighi M-Moderate V-Very Much)
'
EFFLU EN T A D JU STM EN T/PO ST-A D JU STM EN T M EASUREM ENTS:
ACUTE
Date
\{(//Xm l
1111
II
Temp. (C) | S i
1
1
1
1
1
1
II
Salinity (g/kg) | ^ a I
111
1 II
D O. (mg/1) 1 7 - .< P |
1 1 1 1 1 II
Aeration Rate | .--. |
1111
1 II
Aeration Time (min) j ------ |
1 1 1 1 1 II
Adjust. D O. (mg/1) | | 1 1 1 1
II
pH 1%. O f 1 1 1 1 1 Tot Chlorine (mg/1) | | 1 1 1 1
II II
Initials
\
1
1
1
1
1
1
II
DILUTIO N W A TER CH A RA CTERISTICS:
DMW:
HWM: 1 /
Date Ili('/?/^Tl
11l 1 1
Temp. (C) | > . , ? 1
111 1
Conduct. (uMHOS) |
|
1111
Salinity (g/kg) |
|
1111
PH l f f . 3 / 1 1 1 1 1 1
Diss. Oxygen (mg/1) | jL . ^ |
111
*--J
Hard (mg/1 CaC03) | | 1 1 1 1
Alkln (mg/1 CaC03) | | 1 1 1
1
Initials
1 Cr7( 1
1
11
1
1
ACUTE
II II II II II II II II II
EFFLUENT ID: j ft I HrW
JTEST ID: T / I / 9 Q l l Y i f
D-213
US00001394
N 0U -1 9-1 S 9 7 13:11
JR
Fariiiry.Q U
Toxicity Test Sample Chain of Custody (Please complete all information)
Address . N p r j l r ^ , V/V
3
i j o r - ) i<4Do
County_
NPDES#
Pipe/OutfaU/Location Instream Waste Cone
Sample collected by (print<fesign)vjJoOQ
Type of sample S .(Grab):
Date__
AffiliationJ) PU b r\f. En fT. LqU
-- Time %*3 0
J
.(Composite): From Date,
Time,
To Date
Time
Subsamples comprising composite:
Number.--------- _ Frequency of collection. Temperature of sample in sample collection device O Fina! temperature of effluent at sample collection point 3 . 3 . V C ,
Volume
Ts sample collection device c h i l l e d ? ^ . Is sample packed on ice for shipment? M S
Tt is required that all samples remain at 0 - 4*C during collection period and shipn*nr for
data to be accepted by the appropriate Regulatory Agency (Do not freeze!)
Is rhe sample chlorinated?-- * '3 dcchlorinatcd? KO If so, how? V a , ________
Pemur wuh interim chlorine limit?. - [f vcs _
'
Field pH-- 7. %3j --
Field Total Residual Chlorine______
* -------- - j J *r*- .* ___ !...
ivi
Type of tcst(s) to be performed (Specify organisms)
C hrirtl p (V i ICYTnCclort
J
Print & Sign Names
I.
Relinquished by v V e r y i . f e , J o s Q / v ,
D a te ' V / W / f O
Time. \Q -C Q a rO
Received b y D
a re
/ / / / ? # 7 Time.
Relinquished
, Daie >1h i c n Time
Received bv
---- Daic J / / / i / < h Time / / 2 o
FOR REED LAB USE ONLY
SKA#
Color_____ _
Arrival Temperature._____ ' O dor_____
P H _____________ DO (mg/L). ---_ Conduaivity (jimhos/cm).
Salinity (ppt)_
TRC (mg/L).____ Method ____
On ice? . Solids. ____
D-214
* 5/14/9
US00001395
US00001396
-*n . . . . v - u .
i u,
H u o U i ii_ y
& ANALYSIS REQUEST FORM
PWC en v ir o n m en t a l la b o r a t o r y
CODE 930 BLDGZ-140
9742 MARYLAND A VENUE
ENVIRONM ENTAL
NORFOLK, VA 23511 3095 PH: (757)445-8851 FA X : (757)445-8852
LAB USE ONLY
LINE ITEM |S A M P L E N O
m t N i iN t-U ttiv iM i i u i s
*T) <N Q
D -216
US00001397
Coastal Bioanalvsts Inc.
ODU/Oceana A cute Effluent Toxicity Tests
M ysidopsis bahia & Cyprinodon variegatus
Sample I.D. B2 Contract No. N001S7-93-D-9748 * Delivery Order No. 0235 J O. No. 1912290, Item No. 0002BJ, 0002BH, 0003AD
PWCNo. 1375 11/17/97 1910 Sample No. JRA97-13125
Submitted to: Ms. Merrill Anderson-Ashcraft
Navy Public Works Center Code 930, Building Z-140
Norfolk, VA 23511
s
By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062
(804) 642-0168
D-217
US00001398
ODI.' OCEANA Sample I.D. B2 11.1797 Sample 97-13125 Test ID: J R ,\9 7 N 9
Coastal Bioanalysts Inc.
I. TEST RESULTS
% Effluent Concentration
0
6.3
12.5
25.0
50 0
100
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acute Test Results
Mean (%) Organism Survival
M. bahia
24-h
4Srh
C. variegalus
2LJi M
100 100
100 100
100 100
100 100
100 100
100 100
100 100
100 100
100 100
100 100
80 45
100 100
93.9% N/A Trimmed Spearman-Karber 1.06
'
>100% N/A N/A <1.00
Acute Test Information:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
M . bahia Test
48-h Static
Survival
s
11/18/97 1820
11/20/97 1820
CBI stock cultures
11/13/97
20 g/kg
19.2-20.8C
No
N/A
C variegatus Test 48-h Static Survival 11/18/97 1820 11/20/97 1830 Aquatic Bio Systems, Inc.
11/15/97 20 g/kg 19.2-20.8C No N/A
D-218
US00001399
ODri-DCEANA Sample I.D. B2 11.17 97 Sample 97-13125 Test ID: JRA97149
Coastal Bioanalysts Inc.
Sample Collection Pate/Tims
11/17/97 1910
Sample Use Dates/Times
11/18/97 1820/18203
Aeration2 Rate/Time
n/a
Pretreatment/ Adjustment
HWM Salts
2Aeration often necessary to reduce sample oxygen concentration to 100% saturation after warming.
3 bahia/C. variegatus test time.
-
Table 2. Effluent and Dilution Water 4 Chemical & Physical Characteristics (Mean Value (Standard Deviation))
Parameter
Temperature (C)
Salinity (gAg)
Conductivity (uMHOS/cm)
pH
Dissolved Oxygen (mg/1)
Total Hardness (mg/1 as CaCOj)
Alkalinity (mg/1 as CaCOj)
Total Chlorine (ing/I)
n h 3-n (mg/1)
Initial Effluent
4.0 (-)
2 (-)
2400 (-)
8.02 (-)
10.1 (-)
163 (~ )
301 (-)
<D.L. (-)
9.0 (-)
Adjusted Effluent 20.3 (-) 20 {-) N/A
7.86 (--) 7.8 {-) N/A "
N/A
N.D.
N.D.
Dilution W ater 20.4 (--) 20 (--) N/A
8.23
(...)
7.9 (-) N/A
N/A
N.D.
N.D.
4EfHticnt parameters measured upon receipt of sample in lab and after adjustment on day o f use. Dilution water (Wimex hw-Marincmix in ASTM Type I deionized water) values measured after preparation of diluent on day o f use.
_____________________________ l __________________________________ _________________________________________________________ _________ .__________________________
D-219
US00001400
ODt VOCEANA Sample ID . B2 11/17. 97 Sample 97-13125 Tesi ID: J R ,\9 7 149
n . WATER QUALITY
Coastal Bioanalysts Inc.
Parameter
Temperature (C)
DO. (mg/1)
pH
Salinity (gAg)
Control
20.4 (0.1).
7.8 (0.1)
8.01 (0.19)
20 (0)
Table 3 Water Quality Summary
M . bahia Test
Mean Value (Standard Deviation)
2%
20.5 (0.2)
12.5%
20.5 (0.2)
25%
20.5 (0.2)
50%
20.5 (0.2)
7.5 (0.1)
7.98 (0.11)
20 (0)
7.3 (0.1)
7.98 (0.09)
20 (0)
7.0 (0.4)
8.02 (0.04)
20 (0)
6.8 (0.5)
8.05 (0.12)
20 (0)
Parameter
Temperature (Q DO. (mg/1)
pH
Salinity (gAg)
Control
20.4 (0.2)
7.7 (0.2)
8.10 (0.11)
20 . (0)
C. variegatus Test
Mean Value (Standard Deviation)
6 3%
12 5%
25%
0%
20.4 (0.2)
7.5 (0.1)
20.4 (0.1)
7.4 (0.2)
20.4 (0.2) a
7.0 (0-4)
20.3 (0.1)
6.8 (0.5)
7.99 (0.12)
7.99 (0.08)
7.99 (0.02)
7.98 (0.06)
20 20 20 20 (0) (0) (0) (0)
100% 20.5 (0.2) 6.5 (0.6) 8.06 (0.17) 20 (0)
100% 20.3 (0) 6.6 (0.7) 7.95 (0.08) 20 (0)
D-220
i
US00001401
ODU'XTEANA Sample I D. D2 11-17/97 Sample 97-13125 Test ID: JR.X97U9
ID. OUTFALL DATA SUMMARY
Coastal Bioanalysts Inc.
Test Date
9/30/97 11/3/97 11/18/97*
Table 4. Cumulative Data Summary - Acute & Chronic Tests (Tests conducted on this outfall by CB1)
Invertebrate
Acute Tests
Chronic Tests
LC50 T.U.
NOEC T.U.
66.0% 50.0% 93.9%
X
1.52
2.00
1.06
N/A N/A N/A N/A N/A N/A
Vertebrate
Acute Tests
Chronic Tests
LC50 T.U.
ft-OE.C T.U.
>100% >100% >100%
<1.00 <1.00 <1.00
N/A N/A N/A N/A N/A N/A
` Results submitted with this report
IV. QUALITY ASSURANCE
M. bahia - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
C variegatus - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
CBI Stock Cultures 11/20/97-11/22/97 KC1 (Sigma "Ultra" Lot#76HI0391) 343, 490, 700, 1000mg/l 100% 574.8 rng/1 (532.7-620.0) 610.6 mg/1 (488.4;732.8)
Aquatic Bio Systems, Inc. (same batch as test) 11/18/97-11/20/97 KC1 (Sigma "Ultra" Lot#76HI0391) 432, 720, 1200, 2000 mg/1
100%
1029.5 mg/1 (881.7-1202.0) 1079.5 mg/1 (896.5-1262.5)
D-221
US00001402
CUMULATIVE DATA SUMMARY NPDES Permit #: N/A Oceana - ODU B-2
Supplement
].
D-222
'
'
' -I
US00001403
CO ASTA L BJOAN.ALYSTS. INC. N P D S STATTC A CUTE/SA LTW ATER
(Form A M B48)
\ Efflww
t1.0 .
D>r 1 In
CO f O
Day 2
# 1/*
to
finS 1 Survrval
C-2 > b
I O ______
l ce
'1-1
0_____
L$.25
12 ____ _______
I0
iC o
( 2-1 i
12.5
2-2 > 6 ....
>6
(C o
3-1 I O 2S 3-2 > 0
4-1 1 f) 50 4-2 M )
/(/
KI O)
to
l CO
10O
5-1
100 5 2 1 -
4 5_ J _________ ,
Otearvar i t t i
C tf
I M S --Time:
Q o ___
Mvsiopsis bahia
Scure;
C8I
Hatch Dat^Tum :. Acclimata w a te r HWM ASW U S O tlar.
_ Salinity: Z . O
AcclifTottoft Tempi ( r a r ^ e ( 1 3 , ________
2 o ir
F tadrq pnor to test: A nvnu X > c J d Q tW ;_
faadng daing IB I: Nnt lad D
in * TM * j ' *
/d O lia r _________________
C hM itertin: 300 ni p 3
0Ib i dm: W0
5 0 0 mi Q
250rrt Q
1000 ni Q
500 ni
C ib a r ._________ n i
niOth*-._________
tfklial no. or^am xrs par n r f a n : f
^
-------
Photopanod 161:80 & Dt)w:_________ Ra m is i r t icn t a r s i a ta no.
SET UP: P ara
/ t ) ( ( A ' l Tal W atarAdfrd
Tura A n n a li Adthd /
Ci
Sal'U p Br f i U>/ (j~ K
INSTBUMENTAT10N USED;
Slem Da rrrw T aw Ntaiar. M .
YS 0 .0 . M alar f 1 |SNE801447S) CP O ignam a pH M atar (09500563)
I S m o n ta RI60P Balanca [40020093) I YS SCT 33 Matar IH80I6792)
SY S D.0. M atar 1 2 (9 B 1A 026329) Corning 2 4 0 (H M atar (SNS268)
3>P o r t a l o M atar (8523601 f > ] Spartan ATC R a l n c u n a u r (A36S)
0y 0
Day 1
Oay 2
C 3 0 .7
7a r
<3 o - 3
T 1 3 0 .7 A r> 7 io .?
E 2 i .5"
?
P
31 3 0 -3
3 o . 2
7 o .V
4 D 0 -3
ao 2
3 0 .Y
5 3oJ*>
r? ( ' $,
A
C
1 Z .1 1
? .7 f > 9 5*
h?? l- .fi
2 ,o 7
7 -/ri
fH
3 7 A ? 5 UQ>
H.0<
4 1 -M
M
s.d
S 7 - 8 (o &(?> T T T ~
C . l.K
1 %r
7 .j
U
7 -S ~
0 .0 . [ngH
2 3
0 -5 "
>. X 7 -3
? ? 7 -^
4 ? -7
5 r ,?
s
C
<X{~)
-V r>
A1 l
n
2
J -o
iv ,
ti 3 r 3 D
A) 0 ^o 3 t> _
^ ^ ____
4 5 Replicete *
Inbalc
& t>
rQ A 7
^ 2 _ Q _____
38
I A J _______
{ r i i ____ 7 ______ i > / A
NOTES;
(hafciy A oiranca:
A s Q&tf J i n u w i o f f i c e * for P e s t a s t i c e r n ir t h a t nosigfthcani tW iabo Irern K c ep ted pnteeak occir ra d 0*109 m o n a inspect
{ J a oPrtw taa m tad b b w ) and th rt tha U accurata^ railact th
t a t i a a i t w r t p^ cmiod t y C8i.
.
C annatiti
d 9 -- 5 7 " fe
i N /-w p& - / 9
u lp h ~ i
TEST I.D.: "T R A ? 2 / V 2 .
D-223
US00001404
TRIMMED SPEA R M A N -K A RB ER M ETHOD. V E R S IO N 1 . 5
DATE:
1 1 /1 8 /9 7
TOXICANT : B2
S P E C IE S : M BAHIA
RAW DATA :
C o n c e n tra tio n
(% ) .0 0
6 .2 5 1 2 .5 0 2 5 .0 0 5 0 .0 0 1 0 0 .0 0
SPEARM AN-KARBER T R IM :
T E ST NUMBER: JR A 9 7 1 4 9
D U R A T IO N :
N um ber E xposed
20 20 20 20 20 20
45 .0 0 %
M o rta litie s
0 0 0 0 0 11
SPEARM AN-KARBER E S T IM A T E S :
LC50:
95% ARE
9 3 .8 9 CONFIDENCE L IM IT S NOT R E L IA B L E .
48 H
I I
D-224
US00001405
coastal bioanalysts. inc. npdes static acute/saltwater
(Form ACV4S)
% Efflutnl
1.0.
0*y 1
9 In*
Control
6.25 I2.S 25
C-l / P
1 0C-2 1-1
1-2 t
n u>
2 2 ! L>
31 I
3-2 f
4-1
SO 4-2 * b
5-1 ) 0 100 5-2 1
Ot&*rv*r
C 'i-f
Tirr:
Dy 2 9 Ltv*
fO
'( fa 0 U) ...... >(i
1a . '0
(d
10
>0
C
f <v ' 2 c t
final*. StrvrvW
lo o
( Oo
l t> tO b
\ CO
too
io*;TM- C v p h n o d o n v a r ie o a tu s
w >:
-C W /4 A T
H itch Data/Tiim:
I V ) l^ |
Accliiretion witH*. KWM ASW ^
Cthoc
SeTwiiy: 2 _ 0
Accbmatron Tama (rana*):
1i --
io l o . K
F*#dng pner To t* sc Arr*rra J >yC / J
F w ng djnng t**c Not fod S I
A riani*
/d Other
C harter rtfa: 3 0 0 .* Q
SOOrl 0
1 000 rH
o th
Ter. t u r * 2 0 0 *
2S0* 0
0 th
nt ^ r* /
Imitai no. organam i pm r o d i o t e : ( O
Ml repGcetec
R o to p m o d 16LB 0 | 3 "Other
R ndm ntiM tm ite i n a V 'o i j 3
SETUP; Dm i l / / l l 4 1
TV W ater A d id I 7 5 3 ~
Tm A nnbA dM I ^ ^
SetUoBv P O / f c r K
INSTRUMENTATION U S O :
.
Stem T W irarm iar N w im r f i r I
_ I YS 0.0. M m r [S C 8014475) CP C h ^ u fM pH M eter [D 9500563)
D Sertodm Hl 6 0 P Balance (40G2093) L j Y S SCI 33 M eier (H8015792)
Y S 0 .0 . Melar 2 (S 81A 026329) O Con 9 240 (H Melar 1 9 6 2 6 8 ) O SP P o rt t Mater [857360) pC j S t a r t ATC R efrecterm ter (A366)
Other
DayO 0y1 Oay2
p-Vc . 3
r' } (> \r. P . 2 !
1 7 1. v
A (i< = 7
^)<i> J
2 4) ~7~ 7 p .T a i
3 u .)
7 It i n 7 . 3
4
5 -la . \ C
3 .7
P n. 5
% L>
P a .3 P a .3 *>!
1 * -u
in
2 T ? - 7,1* 7 ^ /
3 7-f r k . o t 1 - .4
4
.it
? ,b i
%. C
S ? > tc * 1 -.1 1 f . p i
D.O.
IdT
c 1*1 1 h* 2 .
3 U S'
*-<D 7 -3
25-
7.V
7 .p
4 1 71
5
$ C 3a
A1
l I2
r3 ^b
a7 - 0 u 3a
Qo
N3
(OAfll 4
5 cat* 9
bait:
= ? r>
<3 I
>
5d 30 3l
3o
3A
3 r. J Hi
NOTES:
r Y > ^> - * __< % /'J __/*/O ltr,'t l*77=
on 5. S' a/- ______
TEST I D.: < T L f \ ri 3 / ^ <r
JD-225
US00001406
COASTAL BIOANALYSTS fNC .EFFLUENT/DDLUTION W A T E R D A T A (Fom EFDL0I95)
IN IT IA I (ARRTVAL) EFFLUENT CHARACTERISTICS:
Sample Date 1U/7 77^01
1
Temp. (C) 1 V . 1
1
Conduct. (uMHOS) & b I
|.
1 |
""
11
11 | .\
1
1
j
Salinity (g/kg) | ,Q
|
|
I
l
I
|
pH 1
1 11
Diss Oxygen (mg/1) I ' M
|
1
111
Tot. Chlorine (mg/1) 1 | 1
11 1
NH3-N (mg/1) 1 9 - 0 1
1
1
1
1
1
Hard (mg/1 CaC03) 1 / 6 i | 1 1 1 1 1
AlkJn (mg/1 CaC03) 1 3 ^ 1 1 1 1 1 1 1
Color/Appearance1 I U
1
1
1
1
Initials
111
11
1 C-Clear O-Opaque Y-Yellow B-Brown Bl-Blaci G-Green T-Turbid SI -Solids (S-SIight M-Moderate V-Very Much)
"
'
EFFLUENT ADJUSTMENT/POST-ADJUSTMENT MEASUREMENTS:
Date
11
1
Temp. (C)
Qo,\ 1
1
1
1
Salinity (g/kg) Q b ! 1 1 1
D O. (mg/1)
T .g 1
1
1
Aeration Rate
--I
1
1
Aeration Time (min) "
1
1
1
Adjust. D O. (mg/1) ------ 1
1
1
PH 1 1 1
Tot Chlorine (mg/1) | A/r o | 1 1
Initials
j O fi 1
1
1
DILUTION WATER CHARACTERISTICS:
DMW:
1 1 1.
HW M :v_/
1 1 1
1
1 1 1 1
ACUTE 1 II 1 II 1 II
1 ' 1
1 II 1 II 1 II 1 II 1 II
' II
ACUTE
Date l i i / w n l
1
1 ' 1 1 II
Temp. (C) | a o '.v l 1 1 1 1 1 . II
Conduct. (uMHOS) |
1
1 1 1 1 II
Salinity (g/kg) | Q l I 1 1 1 1 1 II
______E________ 1_____________
1 1 1 1 II
Diss. Oxygen (mg/1) | , ^ - f 1
1
1 1/
II
Hard (mg/1 CaC03) | AlO- I 1 1 1 1 I- II
AlkJn (mg/1 CaCC3) | 1 1
1 1 1 II
Initials
| i'-K 1
1
1
1
1
II
EFFLUENT ID: 6 A
/3 7 5
TEST ID j R f ) * \ V ! l D-226
US00001407
N0U-15-1SS7 13-10
JRfl
Toxiaty Test Sample Chain of Custody (Please compr--let-e 'a**i*l uinaifvojrmation)
757 S73 1498 p . 14
County_~ NPDES#_ _ _ _ _ _ _ _ _ Sample'collected by (print&s
Pipe/OuxfaJl/Location Instrcam Waste Cone
Type of sample (Cmh)-
" /n /^ -r
___7 1 O l M
-(Composite): From Date.
rime -
To Dare
--___ Time --
Subsamples comprising composite:
. . Number--------------------- --- F1requency oorf ccoolllleeccttiioonn..___ _
Temperature of sample in sample collection device ^ Q gQy
Volume
Final temperature of effluent ax sample collection point J l A
Is sample collecrion device chilled7
r5 mot, ,, ^ ^ -
-t, -
.,
~ L * - 1 mple; packed on ice for shipm ent7 KAP5
It is required th a t all sam ples rem ain at 0 - 4C
i. -
p ra e n t._ ^ _ _
r: -* r
r r :::r
IS the sample chlorinated?_^_ cechIorinated?_KO u ,, faow,
for
Pcnnttwith intermi chlorine limit?------1 _ If yes - limit (mg/L)
~
Field pH-JLOQ--
field Total Residual Chlorine
:'
Commenrs/Samplc d cscn p t:0a_ g 1 J ig j p p J ^ F + W t W c
Type of test(s) to be pcrfonned__tju&j[^_<^n ^ p . C ruCWnQ/Jofl
(Specify organisms)
X /flr C P ^ fii <>
3*
Method of shipment C^UVOr^ Q C \A
1. Relinquished by
Print & Sisn Nam es
C S O _ Dare w / H / c g
Time \ Q - C Q a r O
-.
D z t c _ f / 1 a g f c j -7 rime H
FOR REED LAB USE ONLY
JRA*_ 3 7 - m x < r
. Arrival Temperarure.________
Color_____ __________ __ __ Odor...______
--------- --- DO (mg/T,). ---- Conductivity (jrmhos/cm).
Salinity (ppt)_
TRC (mg/L)__
Method________
1r
On ice? . Solids.
_I
n /Q ,
D -2 2 7
a* 5/1/}*
US00001408
US00001409
L/fiHiiM -ur-L/U I \ju r n c o u n u
ENVIRONMENTAL
& ANALYSIS REQUEST FORM
PWC ENVIRONMENTAL LABO RA TO RY CODE 930 B LD G Z-140 9742 MARYLAND A VENUE NORFOLK, VA 2 3 5 1 1 - 3 0 9 5
PH: (75 7 )4 4 5 -8 8 5 1 FA X: (7 5 7 )4 4 5 -8 8 5 2
TYPE G -GRAB CF - COMPOSITE, FLOW CT. COMPOSITE, TIME
M A T R IX
L . LIQUID
S W -SW IPE
8 - SOLID
O S . GAS
S S -S E M I SOLID
P -P L A S tl^ fi
A -MBR(gl8I I I
GL i GLAS C i CARTRIDGE :
f: ,T -TEFLW T'We FLOH LlHED LID '. '
,v v o A T^^ hexan rinsed.:,, Y t :
PRESERVATIVE
1 -C O O L TO 4 C
6 HCL, pH<2
2 -H N 0 3 , pH<2
6 0,008% N*2S203, 4 C
3 - H2S04, pH<2
7 FIELD FILTER
4 NOH, pH>12
i-N O N E
f\MHAtOUHD (D iri):
COMMENTS:
(FOR R U SH TURNAROUND STATE R E A SO N BELOY/)
REGULATION APPLIED:
RCRA ( ) SDWA ( )
HRSD ( ) TSCA ( )
CWA ( ) PHOTO ( )
CAA ( ) OTHER ( )
SAMPLING/COILECTION CHARGE: POSSIBLE SAMPLE HA2AROS: COMMENTS:
4
SAMPLE DISPOSAL: ( )RETURN TO CLIENT ( )DISPOSAL 0Y LAB
BTn iUMBER:---------------------- INIT.
CONTRACT LAB:--------------------------- DATE
/. CUSTOMER IS RESPONSIBLE FOR ALL CHARGES N EC ESSA R Y FOR THE PROCESSINO AND ANAL YSIS OF SAMPLEIS).
ALL RUSH SAMPLES ARE SUBJECT TO SURCHARGE.
ONLYCONTRACT NO.(S): W IL 'M L U S E
7. SAMPLES RECEIVED AFTER 2:00 PM MON. - THURS, AND 1:00 PM ON FRIDAY WILL BE PROCESSED THE NEXT BUSINESS DA Y 17:00 AM 3:30 PM).
"F O R LAB USE ONLYII
R ejected ( )
r e a s o n -, h o ld in g t im e i )
m n iu aiS H tu u Y .''
}[TTFXIHOUISHEt)-HYr
rRErmoTSTEtrw
3z-
SAMpLES.VEttiFieATlDN ;
c o n t a in e r s ; )
im p r o p e r l y s a m p l e d ; ) o t h e r ; )
W mpny/uuMmnu.}
.
JLX-X
TUMPANY/UUMMANUi ' /A - X
.. YTM PANY/UUMMANU:'"' "T T T
`",:TUMPANY/UUMMANU:
INITIAL:
OATE: ~
TTT
J-LQ o
"DATE IIMbT/7Y/!/vx)lVy
l
"UATb/IIME
lJB
TJXTE) I I M I T t Ti v -a^l i T i
T Q
Coastal Bioanalysts Inc.
- ODU/Gceana
Acute Effluent Toxicity Tests
M y sid o p sis bah ia & C yprin odon variegatu s
Sample I.D. B3
Contract No. N00187-93-D-9748
'
r Delivery Order No 0235
J O. No. 1912290, Item No. 0002BJ, 0002BH, 0003AD
PWC No. 1376
11/17/97 1930 Sample No. JRA97-13126
Submitted to: Ms. Merrill Anderson-Ashcraft
Navy Public Works Center Code 930, Building Z-140
Norfolk, VA 23511
By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062 .
(804) 642-0168
D-229
US00001410
ODU'OCE.XNA Sample I.D. B J 11,17. 97 Sam ple 97-13126 t e s t ID: JRA 97150
Coastal Bioanalysts Inc.
I. TEST RESULTS
% Effluent Concentration
0
6.3
12.5
25.0
50.0
100
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acute Test Results
Mean (%) Organism Survival
M . baha
C. variegatus
24-h 4gJi
24-h 48-h
foo 100
100 100
100 100
. 100
100
. 100 100 100 95
100 100 100
15
100 100 100 100 100 100 100 100
75.2% N/A Trimmed Spearman-Karber 1.33
>100% N/A N/A <1.00
Acute Test Information:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
M . b a h i a Test
48-h Static
Survival
*
11/18/97 1840
11/20/97 1840
CBI stock cultures
11/13/97
20 g/kg
19.2-20.8C
No
N/A
C v a r ie g a tu s Test 48-h Static Survival 11/18/97 1840 11/20/97 1850 Aquatic Bio Systems, Inc. 11/15/97 20 g/kg 19.2-20.8C
No N/A
i
D-230
US00001411
oDU OCEANA Sample ID . B3
11,17 97 Sam ple 97-13126 Test ID: JR A 97150
Coastal Bioanalysts Inc.
Sample Collection Date/Time
11/17/97 1930
Sample Use Dates/Times
11/18/97 1840/18403
Aeration2 Rate/Xiipe
n/a
Pretreatment/
Adjustm ent
HWM Salts
2Aeration often necessary to reduce sample oxygen concentration to 100% saturation after wanning. 3_W. bahia/C. variegatus test time.
it Table 2. Effluent and Dilution Water 4 Chemical & Physical
Characteristics (Mean Value (Standard Deviation))
Parameter m
Temperature (C)
Salinity (g/kg)
Conductivity (uMHOS/cm)
pH
"
Dissolved Oxygen (mg/1)
Total Hardness (mg/1 as CaCO j)
Alkalinity (mg/1 as CaCOj)
Total Chlorine (mg/1)
n h 3-n (mg/1)
.
Initial Effluent
2.9 (-)
2 (-)
"" '!r 2400 (-)
. L't irr~
7.99 (-)
10.1 (-)
lo t 145 (-)
361 (-)
<D.L. (~ )
9.6 < -)
Adjusted Effluent 20.5 (-) 22 (-) N/A
7.78 (-) 7.8 (-) N/A N/A
N.D.
N.D.
Dilution W ater 20.4 (-) 20 (-) N/A
8.23 (-) 7.9 (-) N/A
N/A
N.D.
N.D.
4Efflucnt parameters measured uppn'receipt of sample in lab and after adjustment on day of use. Dilution water (Wimex hw-Marincinix in ASTM Type 1deionized water) values measured after preparation of diluent on day of use.
i v ii
D-231
US00001412
ODl'-'OCE.-VNA Sam ple I D. B3 11- 17,'97 Sam ple 97-13126 Test ID: JR A 97150
Coastal Bioanalysts Inc.
n . WATER QUALITY
Parameter
Control
Temperature (C)
20.3 (0.1)
DO. (mg/1)
7.8 (0.1)
pH 7.87 (0.32)
Salinity 20
(g/kg)
(0)
Table 3 Water Quality Summary
M . baha Test
Mean Value (Standard Deviation)
6 3%
t:
20.3 (0.2)
12*2%
20.2 (0.1)
25%
20.3 (0.1)
50%
20.3 (0.1)
7.6 (0.2)
7.83 (0.20)
20 (0)
7.4 (0.2)
7.85 (0 17)
20 (0)
7.2 (0.2)
7.93 (0.06)
20 (0)
7.0 (0.1)
7.99 (0.14)
21 (0)
Parameter
Temperature (C) DO. (mg/1)
pH
Salinity (g/kg)
Control
20.3 (0.1)
7.8 (0.1)
8.10 (0.11)
20 (0)
C. variegatus Test
Mean Value (Standard Deviation)
6 3%
12.2%
25%
50%
20.4 (0.1)
7.7 (01).
7.81 (0.27)
20.3 (0)
7.4 (0.3)
7.82 (0.23)
20.3 (0.1)
7.2 (0.2)
7.89 (0.07)
20.3 (0.1)
7.0 (0.1)
7.92 (0.08)
20 20 20 21 (0) (0) (0) ()
I% % 20.3 (0.2) 6.9 (0.2) 7.99 (0.19) 22 (0)
100% 20.4 (0.1) 6.8 (0.2) 7.90 (0.10) 22 (0)
D-232
US00001413
O D W X 'E A N A Sam ple I.D. B3 I I 17 9 7 Sam ple 9 7 -1 3 1 2 6 Tesi ID: JRA 97150
IH . O U TFA L L D ATA SUM M ARY
Coastal Bioanalysts Inc.
le st Pais
9/30/97 11/3/97 11/18/97*
Table 4. Cumulative Data Summary - Acute & Chronic Tests (Tests conducted on this outfall by CBI)
Invertebrate
Acute Tests
Chronic Tests
LC50 T.U.
NOF.C T.U.
65.0% 45.0% 75.2%
1.5^ 2.22 1.33
N/A N/A N/A N/A N/A N/A
V ertebrate
Acute Tests
Chronic Tests
LC50 U L
NOF.C U L
>100% >100% >100%
<1.00 <1.00 <1.00
N/A N/A N/A N/A N/A N/A
Results submitted with this report
IV. Q U A L IT Y A SSU R A N C E
M . b a h i a - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
C v a r ie g a tu s - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
CBI Stock Cultures 11/20/97-11/22/97 KC1 (Sigma "Ultra" Lot76H 10391) 343, 490, 700, 1000 mg/1 100% 574.8 mg/1 (532.7-620.0) 610.6 mg/1 (488.4-732.8)
Aquatic Bio Systems, Inc. (same batch as test) 11/18/97-11/20/97 KC1 (Sigma "Ultra" Lot#76H 10391) 432, 720, 1200, 2000 mg/1 100% 1029.5 mg/1 (881.7-1202.0) 1079.5 mg/1 (896.5-1262.5)
D-233
US00001414
CUMULATIVE DATA SUMMARY
: : *f
" NPDES Permit #: N/A '* Oceana - ODU
B-3
VefTET t
W W B& &
I 2/12/97 >100
>100
| 2/28/97 Invalid
> 1,00
| 3/12/97 >100
>100
| 3/26/97 >100 | 9/30/97 65
>100
-------- t -
>100
1 11/3/97 45.0
>100
| 11/18/97 75.2
>100
^
--Invertbraie-viir^e'-
TJOEC%
NOEG %-
:. . A
1
Supplement
D-234
US00001415
COASTAL BIOANALYSTS. INC. NPDES STA TIC ACUTDSA LTW ATER
(Form AMB48)
lEIH u** ,
t.O.
Contra) 6.25 12.5
C-! C-2 1-1 1-2 2-1 2-2
1*1 25 3-2
M 50 4-2
5-1 100 S-2
Observe
0Y 1 9 Uv
fo
D n2 Uv
/r; -
i O _______ - _ J L _________
__ L61 0 ________ f 0 __ L2___ <o . / 0 0 /r> _
1 0 .. _JJ2____
1 o __ LQ___ 1 0 . _ J J 2 ___
1 II ..
-------------
1 C -H
___________
3
G ff
Tina:
fra il
1c 0
iO t 1 Oo
iO O
too IS
Oay 0
P*Y 1__________ 0 r 7
C Q o . V A o .y 2 & 1 ...
T1
t> .a _ -M .A
E
M
2 *i>.3 3
^ tf
7 5-3 <35. ?
4 a o -3 o) >- 3 Q p - = P
5 StG.S A A .2) 3 , J
C
1 2 H 3
4
5
c 1 20 .0 . tn^II 3
4
5
sC A1 l2 N3
(rW 4
5
Bpfc-< 9
l n tote;
W8. 7- 0 G
& n a T-.8 / ( f i 7 . 7
7 - . S 7, R.S Y . S"
b .7 X
7-A
? . 3: lo
9 /3 -
77 ?-<L_ ? y _ 7 - 3 - 7-?
3 -H r.-q
-fe'. (. h
f> - /)
T.J>
?-r>
7 .
3h
.T o i-
? . -0 s
Q^ IAe-- 3 D - ^ A_____ O/) 5 .
.37
_ J J l i_______
________
_
i3 UH _ 9 H
____
&H
wir. O 0 P . fo ftP-----f Y Y ^ Z -
(^afy Auunnc*:
As O w tn A n u n n o olfieor lor dos l u t 1 n t t y liw no y s t e
* M O on> hon. sc ro tM p ra to c ri c o m d * `n" 9 " `T l ^ ! L
(B rini B h > M now ) tolo~l I " 1 It* *>
m t m it w m parfom d by CBL
a"
.
- r \ ------------^ -- y 7-- P T T
^
/J v y c a c o
7
i 3 7 X
TESTI.D.: - f ( l A 1 7 / < V > D-235
US00001416
TRIMMED SPEARM AN-KARBER METHOD. V ER SIO N 1 .5
DATE:
1 1 /1 8 /9 7
TOXICANT : B3
S P E C IE S : M BAHIA
T EST NUMBER: JR A 9 7 1 5 0
D U R A T IO N :
RAW D A T A : ------- -------------
C o n c e n tra tio n
(% ) .0 0
6 .2 5 1 2 .5 0 2 5 .0 0 5 0 .0 0 1 0 0 .0 0
N um ber E xposed
20 20 20 20 20
20
'
M o rta litie s
0 0 0 0 0 17
SPEARMAN--K A R B E R T R IM :
1 5 .0 0 %
'
SPEARMAN--K A R B ER E S T IM A T E S :
LC50 :
7 5 .1 7 95% CO N FID EN CE L IM IT S ARE NOT R E L IA B L E . '
48 H
D-236
\
US00001417
CONSTAI. BIO AN A LV STS. INC. N PD ES STATIC ACUT E/SALTW ATER
(Form ACV48)
\ EHJutnr
0*1 1-0. 9 liva
Day 2 fin *
C-l I f
m
C-2 t o
f
1-1 I f )
6.2S
1-2
f
2*1. l
IO
12.5
2-2
l ______
/0
3-* !
to
25 3-2 f f >
1 o___
4-1 io
IO
s o 4-2 6
\ f)
5-1 ! fi
_ 2 _________ _
100
5-2 I O
J L ____
0bsrvr
O -V
G -H -
Tur: ' f i - P O
m iL .
Enal 1
Survival 1C o
i GO iC fc
l^ O
\0 0
lO t> i-:
,
c w im - C vonnodon m riB C ituj
Sourca:
"C8T
Hatch D ata/Tira:
^1 1
1
A cclirm tionw arer HWM ASW
Othar:
Salinirv: 2 --0
to " 2 _ o . K
faanQ trroi to im e A /ttnv J
M Othar
Faadng dunng ta s i: Not fad E ) Artva
/d Othar.
C Ia rta r, : 3 0 0 *
500 ni B
1000*
Ot-.
*.
T aaduna: 2 0 0 *
250* B
SCO ni
O thar
I n tu no. oroantiTS oar ro t e a t a :
1^
Ha-nOaun --
Phctoparw t 16L 80 ^ 3 - v - Othar
R an d x m o A tariffata no. f C*J
SE T U P Data I V
Tio W a tarA d M ( 3 , 0
Tot A n m ls Addad
YO
Sai Ub Bv P O / C r *
INSTRUMENTA TION USED:
Slam Tham nim tar Nurrbar A " 1
T 9 D.O. M .I 1 SNE8014475)
53CP D f W l * M atar (D 9S00563)
O Sanonuo R160P M a n e . [40020093)
O T S SCT 33 M alar [H801E792)
S T S D.O. M atar 1 2 (SN91AQ26329)
O C o rra i 2 4 0 fH M atar (9 6 2 5 8 )
O S PortaH2 U atar 852360)
( * } S parar, ATC R a h ac to n aiar [A366)
Othar
C 1 2 3
4
5 C
1 2 3
4 5 C 1 0.0. 2
(rrgT) 3
4
5 SC A1
L I2
N3
taW 4
5 cat* 1
tiab:
OrrO D .l | .< r 3 iM
Off 1
rO O -4
V-3 f i tifi*
0*2
^0 D 0 f.' Q& ^
l>A 4 0 ~ ?
3 /> Y
frD
?.<r
V
3 o .y
Oo j
"S' . o i
> /S 3
7 T?7 -*f
3 *5 to 7-S I 7 -,i l .
7 -.? *
Z.1
1- w 7 --Y Tn . "f L .U
?-? a .6
17 - . %.)
7 -? . 7? 3 ./
_Ll 1 _
A r> An
A0
<3 0
A 1
^? r> <3, i s
i*>
9 ?
0! n
3'
^ .0 -3A A j3 _
^5 (
aa_ :0
_ 4 ______
NOTES*
r TO Aq - t-* x'A / - a c ir-rv-U .
TEST I.D.: < J . A ^ 2 <fO
D-237
US00001418
COASTAL BIOANALYSTS INC. EFFLUENT/DILUTION WATER DATA iFora. EFDLOW)
IN ITIA L
Sample Date
t / I / / 7 - M ? ! ______________ l
|-
1
1
Temp. (C)
1 . 1 i
i
i
11
Conduct. (uMHOS)
1!
1
1
5?a lin itY ( g / k g )
^
1
t
1
1
1
pH M - ^ 1
l _______!---------------------- !---------------------- !---------------------- ----------------------
Diss Oxygen (mg/l) J . l 1
l 11t
Tot. Chlorine (mg/l) 4 p L 1____________ 1
1 11i
NH3-N (mg/l) ------- I LT> - -----1--------------------- -1---------------- i
11 1
Hard (mg/l CaC03) 1 v s -
1 _________
1
!1----------------
1
1
1 1
1 1
1
Alkin (mg/l CaCQ3)
EFFLUENT ID:J3_3.
Mb
D-238
US00001419
fsL v-iS -lS S T
Faci l i t y-- Address K lP r - C ^ W
Toxjcity Test Sample Chain of Custody (Please complete all information)
U n n f - ^ fr/ \/A - ^
i-i5 o
r .ij
County NPDHS#
-------------------- --
Pipe/Outfall/Location mInMst<rec33m Wwaste Conec
Sample collected by ( p r i m f o i ^ J o S o n ^ g ^ A i f l U
a '^ a l i V
b r^ ljL
Type of saniplc_^__(Crab):
Dale " 7 1 7 / ^ T im , 7 '
3'^
--d -- (Composite): From Date.
To Date Subsamples comprising composite:
" --Tim e " -- ______ Time_
*
_ Number------------------- Frequency of collection_______ 'Temperature o f sample in sample collection device " O o0 j Final temperature of effluent at sample collection point
Volume
i s sample E le c tio n device c h i U e d ? ^ , is sample packed on ice for shipment? ^ S
T t ,s req u ired th a t all sam ples rem ain at 0 - 4*C during collection p erio d an d s h i ^ T f b J
d ata to be accepted by the appropriate Regulatory Agency (D o not freeze!)
Is the sample chlorinated?___........ chlorinated? KO rf so, how ?_ r' A ,
Permit with interim chlorine Limit? -
If ves - limit (m s/L )
~
'
Ficjd pH -----7- ^ V
Field Total Residual Chlorine
1
Commencs/Samplc description B 3 ~ 6 0
A f'F F ' i f e n tP n 'S
Type of tcst(s) to be perform ed___ M \ d
p . C .c a p rin O d n n
(Specify organisms)
V a n '6 fla ,-k t< ? `
- > * ....................
Method of shipment
C V iK
PriTlf
Vo m a e
Salinity (ppt).
Conduaivity (nmbos/cm)_ TR C (mg/L)_______ Method___________
D-239
5/16/9*
US00001420
oriMiiM-vjr-ouo i u u r n a u n u
u - i t m iiMr-ui-uviAt iuin
ENVIRONMENTAL
& ANALYSIS REQUEST FORM
PW C ENVIRONMENTAL LABORATORY CODE 930 BLDG Z-140 9742 MARYLAND AVENUE NORFOLK, VA 23511 - 3 0 9 S PH: 17571445-8851 FA X : (757 )4 45 -8 852
COMPANY/COMMAND:
l-,
CONTACT: tV iu a \ a * Y-
!PrkHiO/NNTEr:. 11 ^ .LLy_ cEvXTT.:
r
P -O -tt SIGNATURE:
0 ^ S ,_'_\_\Z__t__7
PERMIT NO.:
C0PE~ FAX: f ? ?
LAB U SE ONLY
*U N E ITEM S A M P LE NO
SAM PLE ID A O C A TIO N
wnru ON IAA4N rule
At np SAMPLED
# WSAHPU
TIIM HAAU PflCSEAYATfYE a:ohtajnctj
A N A L Y S IS
FIELD READINGS pH TtHPfAATUM
m R.... 1er. J A 7 / ,
/5 .7 a .
A - ! C ot^ U I A " L C o n 4 > o l
SIAM 5I0P SIAM
stop
XD
. rt.Xn
L
1
1
1r
La,.............. \ 0 T l - 3W-I
"h-i iL)>
L
1A 7 A f~V - .A LLn'J-vft iI--. . r w f t - l ,,
STMT STOP -STM T STOP
y\\o
r .......
.....-..MM..,. 97-/
*"----.......- u;
97*
STAM
a 7 r R- a J f i ! \si
I1 0
..................... 97 -
p r p ^ r J STAM
m i p L->~ o
ntb y sioe
cr> ` "blau
f i l l PllVf^J LiULAf*
SIAM STOP
JJMSl -
, __> *
..... 9 7 - fb)a7
PAISCAVAIIOH
vutimoi ClW-
_L_ L _
_1
_L
G -CRAB CF - COMPOSITE, FLOW CT - COMPOSITE, TIME
L * LIQUID
SW-SW IPE :
S SOLID
GS-GAS
S S -SEMI SOLID
P p LIST fiftcV);-': PB .1RBAi fGt :'C;; :
AA .*AAUMRBFEHRI (nglMit*)
GL* GLASS C CARTRIDGE:.
T - T E F L O N 'L;TL'* TEFLON MHe d l id ; ' .
*V ..VOA',": ..il'U' H h e x XMe RNSE . '
1 * COOL TO 4 C 2 HN03, pH<2 3 M2S04, pH<2 4 NaQH, pH>12
5 * HCL, pH<2 6-0.00B% Ni2$2O3,4C 7 * FIELD FILTER 6 -NONE
rutHAAOUMD (Din): COMMENTS:
(FOR RUSH TURNAROUND STA TE REA SO N BELOW)
SAMPLE DISPOSAL: ( (RETURN TO CLIENT ( (DISPOSAL BY LAB
REGULATION APPLIED:
RCRA ( )
HRSD( )
SDWA ( )
TSCA ( )
CWA ( ) PH O TO ( )
CAA ( ) O T H E R ( )
1. CUSTOMER IS RESPONSIBLE FOR ALL CHARGES N EC ESSAR Y FOR THE PROCESSINO AND ANAL YSIS OF SAMPLE(SI.
ALL RUSH SAMPLES ARE SUBJECT TO SURCHARGE.
SAMPLINQ/COLLECTION CHARGE: POSSIBLE SAMPLE HAZARDS: COMMENTS:
$
otiumbek;------------------ INIT.:
CONTRACT L A B :...............
DATE:
CONTRACT NO.(S): vm cm rnsnm ar
2 . S A M P L E S R E C E I V E D A F T E R 2 : 0 0 P M M O N . T H U R S . A N D 1 . 0 0 P M O N F R I D A V W IL L B E P R O C E S S E D T H E N E X T B U S IN E S S D A Y ( 7 : 0 0 A M 3 : 3 0 P M I .
FO R LAB USE ONLYII
REASON:R EJEC TED ( )
fkEUriUOlSTlE'U'BT
(TTonorjisREU-BY
' s a m p l e s V e r if ic a t io n
Y
INITIAL:
HOLDING TIME( )
CONTAINERS! )
a
IHEC'DTTf
IM PROPERLY SAMPLED! ) OTHER! )
DATE: ~
XUMPANYCMMN:r'7 /3 Z T ? " ' ^ ' i l r L'u:TUAifc/irMe
"TREUTTBY "IREtrcrBY
AA j
~LTJMPANY/UUMMANUr ^ / X'OMPANY/COMMATJU"
lTATUiImL:1/ , / im1T1ifclij IMhMiflc":'
jREClTBY
'JL
' UUMPANYJCOMMANP;....Gj~j "
TJATk/IIMt:
So
liL E u . n ln
T<Nt Q
US00001421
Coastal Bioanalysts Inc.
ODU/Oceana Acute Effluent Toxicity Tests
M ysid o p sis bah a & C yprin odon variegatu s
Sample I.D. Mixed Liquor Contract No. N00187-93-D-9748 Delivery Order No. 0235 J.O. No. 1912290, Item No. 0002BJ, 0002BH, 0003AD
PWC No. 1377 11/17/97 1045 Sample No. JRA97-13127
Submitted to: Ms. Merrill Anderson-Ashcraft
Navy Public Works Center Code 930, Building Z-140
Norfolk, VA 23511
8
By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062
(804) 642-0168
D-241
US00001422
O D IV O C E A N A Sam ple I.D. M ixed Liquor 1 1/ 17/9 7 Sam ple 9 7 -1 3 1 2 7 T e si ID: JR A 9 7 I4 4
Coastal Bioanalysts Inc.
I. TEST RESULTS
% Effluent Concentration
0 6.3 12.5 25.0 50.0 100
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acute Test Results
oo
oo
Mean (%) Organism Survival
M . baha
iLh M Ji
b0 100
C. variegatus
24-h
48-h
100 100
100 100 100 100 100 100 100 100 100 100
. 100 100 100 100 100 100 100 100
>100% N/A N/A <1.00
>100% N/A N/A <1.00
Acute Test Information:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
M . b a h a Test
48-h Static
Survival
*
11/18/97 1725
11/20/97 1640
CBI stock cultures
11/14/97
20 g/kg
19.2-20.8C
No
N/A
C v a r ie g a tu s Test 48-h Static Survival 11/18/97 1725 11/20/97 1650 Aquatic Bio Systems, Inc.
11/15/97 20 g/kg 19.2-20.8C
No N/A
D-242
US00001423
O D I' O CEA N A Sample !.D. M ixed Liquor 1 1 17 97 Sample 97-13127 Test ID: JR A 97I44
Coastal Bioanalysts Inc.
Sample Collection Date/Time
11/171 9 1 1045
Sample Use
11/18/97 1725/17253
Aeration2 Bate/Tlme
n/a
Pretreatment/ Adjustment
HWM Salts
2Aeration often necessary to reduce sample oxygen concentration to 100% saturation after wanning. bahia/C. variegatus test time.
Table 2. Effluent and Dilution W ater4 Chemical & Physical Characteristics (Mean Value (Standard Deviation)/
Parameter
Temperature (C)
Salinity ... (gAg)
Conductivity (uMHOS/cm)
pH
Dissolved Oxygen (mg/1)
Total Hardness (mg/1 as CaCOj)
Alkalinity (mg/1 as CaCOj)
Total Chlorine (mg/1)
n h 3-n (mg/1)
Initial Effluent
4.0 (->
N/A
850 (--)
8.28 (--)
11.1 (-)
142 (-)
287 (-)
<D.L. (~> 1.8 (-)
Adjusted Effluent 20.3 (-) 20 (-) N/A
8.18 (-) 7.9 (-) N/A 8 N/A
N.D.
N.D.
Dilution W ater 20.7 (--) 20 (--) N/A
7.95 (-> 7.9 (--) N/A
N/A
N.D.
N.D.
4Efflucnt parameters measured upon receipt of sample in lab and after adjustment on day of use. Dilution water (Wimex hw-Marincmix in ASTM Type 1 deionized water) values measured after preparation of diluent on day o f use.
D-243
US00001424
O11D1I7'-O97CESAamNApleS9a7m-1p3le1I2.7D. MTeixstedIDL:iqJRuoAr97144 n . W A T E R Q U A LITY
Coastal Bioanalysts Inc.
Parameter Temperature
<C) DO. (mg/I) pH
S aiini tv (gAg)
Parameter Temperature
(C) DO. (mg/1) pH
Salinity (gAg)
Control
20.2 (0.7)
7.8 (0.1)
7.88 (0.09)
20 (0)
Table 3 Water Quality Summary
M- baha Test
Mean Value (Standard Deviation)
6 3%
20.2 (0.6)
IL i%
20.3 (0.5)
25%
20.2 (0.5)
50%
20.2 (0.5)
7.8 (0.1)
7.90 (0.13)
20 (0)
7.7 (0.2)
7.94 (013)
20 (0)
7.7 (0.2)
8.04 (0.07)
20 (0)
7.8 (0.2)
8.17 (0.04)
20 (0)
100%
20.0 (0.6)
7.8 (0.2)
8.28 (0.09)
20 (0)
-
Control
20.2 (0.7)
7.9 (0.1)
7.84 (0.15)
20 ()
C. vartegatus T est
Mean Value (Standard Deviation)
6 3%
20.2 (0.6)
7.8 (0.1)
7.92 (0.14)
20 (0)
12.5%
20.2 (0.6)
7.8 (0.1)
7.96 (0.12)
20 (0)
25%
20.0 (0-4)
7.7 (0.1)
8.03 (0.09)
20 (0)
30%
20.1 (0.4)
7.8 (0.2)
8.13 (0.06)
20 (0)
100%
20.1 (0.6)
7.7 (0.2)
8.21 (0.04)
20 (0)
US00001425
OD* ' O C E.ANA Sample I.D. M ixed Liquor il 17.97 Sample 97-13127 Test ID: JR.A97144
ID. OUTFALL DATA SUMMARY
Coastal Bioanalysts Ine.
Test Pate
9/30/97 11/3/97 11/18/97*
Table 4. Cumulative Data Summary - Acute & Chronic Tests (Tests conducted on this outfall by CBI)
Invertebrate
Acute Tests
Chronic Tests
LC5 T.II,
NQEC T.U.
>100% >100% >100%
<1.00 <1.00 <1.00
N/A N/A N/A N/A N/A N/A
Vertebrate
Acute Tests
Chronic Tests
LC5Q U L
NQEC T.U.
>100% >100% >100%
<1.00 <1.00 <1.00
N/A N/A N/A N/A N/A N/A
Results submined with this report
TV. QUALITY ASSURANCE
M. b a h ia - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
C v a r ie g a t u s - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
CBI Stock Cultures 11/20/97-11/22/97 KC1 (Sigma "Ultra" Lot#76H10391) 343,490, 700, 1000 mg/1
100% 574.8 mg/1 (532.7-620.0)
610.6 mg/1 (4 8 8 .4 -7 3 2 .8 )
Aquatic Bio Systems, Inc. (same batch as test)
11/18/97-11/20/97
,
KC1 (Sigma "Ultra" Lot#76H10391)
432, 720, 1200, 2000 mg/1
100%
1029.5 mg/1 (881.7-1202.0)
1079.5 mg/1 (8 9 6 .5 -1 2 6 2 .5 )
D-245
US00001426
CUMULATIVE DATA SUMMARY NPDES Permit #: N/A Oceana - ODU Mix L. R.R.
iDfefbf'#;
I 2/12/97 >100 | 2/28/97 Invalid j 3/12/97 >100 | 3/20/97 >100 | 3/26/97 >100 | 9/30/97 >100 1 1 1/3/971> loo | 11/18/97|> 100
yertbrate.J^i^^ i Invertebratevjr
>100
NOEC%
>1,00
>100
>100
>100
>100 >ioo
-- ;------
' >100
> Vertebrate-- .
|
NdEc:%c^v ^ ^ 1
1
1 1 1 1 11 j --
Supplement D-246
US00001427
C O A ST AJ. BIOAN.Al.VSTS. INC. N PD ES STA TIC A CUTE/SA LTW ATER
(Forni AM B48)
1MIuant
l.D.
0r I
t Uva
On 2
9 l/M
Control 6.25 12.5
C- t o
tO
C-2 i M lo
___ Li___ l
1-2 _ } ____ ___ ' >
/2-1
>>
2-2 l b
11
A13-1
IO
25
3-2 16
___ L i 2 _
4-1 t b
10
11150 4-2
ta
5-1 1
/ O100 5-2
f 1
Obsarvar
Tirm: < ? Y Q . -
final 1 Survival
/ do lOQ 1oc
100
1 Oo (iO
Sn~->c- M vsidoD srs b a h ia
Hatch O atartiira: f J / V / ^ ^ 1
Som a;
CBt
i^H iroron l . r HWMASW Q
Othar:
Satiniry: 2 . 0
A rrhiranon T arm franoal:
r/
2 < i Ir
fa a n q poor to last: A /tW i
V " l O thar
Faadnp dinne test: N otfad O
Artana j - v
l Ow:
D u n iw u ir 300 ni
5 0 0 ni [ j 1000 n i [ ] O thar
ni
Tali voliare: 200 n i 0
2 5 0 n
500 n i ] Othar
ni
I n tu ne. oroanian* per radicata: / ^ ftttu p an o d 161:80 l 'A Orbar
Ma. tnfcctfaa: * 2 -- R n b n m io A tM n lw iB L ^ / '
SETUP. D an
lim a Water A d M / 7 - I
Tum Aramats Addad / ^ " 3 r "
Sal Up By ^ / G - K
INSTRUMENTATION USEO:
S iam Tharmormiar W w it r 3 T 9 0.0. Malar 1 1 ISNE8014475) CP Dignaraa pH M alar {D9500563) Sariorna R160P Baiare (40020093) L J Y 9 SCT 33 M alar (H8016792)
H Y S D.O. U a ta r # 2 (9 fi)A 0 2 & 3 2 9 ) Q Cornng 240 d t U atar (S76268)
SP P o rta r Malar [BS2360) i y i Spartan ATC Rafractematar (A366)
Othar
.
0*Y 0
D*y l_________ O n 2
.T E MI P
PH
C 0 -2 ..S o , ^ 1 D . D in
2 `U
3 V ./
f;
'4 7 / }
5 t>. b J ? 0 f
C -.4 T
.7 /
i.> y
? if
2 *7 o ic 2 7 V
3 ff .rO f
H .V /* < r /< v .2
f.
19 U '9 . t /
/ >7(7 -7 P
-t-.fl
4 tf./V 5 tf.l? C M
S U )/ f .3 r > 3
* /< . ? .*
1 >1
7 - ir
7 .2
0.0. 2 > ?
7 -.?
7-V
(mgfl)
3
7 -S -
4
5 7 -4
S C 2 ^ c ? iO
A ! a 0 2 /. l
12
D "7 6
fi 3 l>
7 - -T ? .> " < 0 /1
a a ...
A z i _______ .a n
4 Xs>
6
...
5
Radicata $
Initials:
A /
1 /L
_ = 2 ________ . _ M ________ E t t -
NOTES:
TEST I D.: ~ r f t A 7 ? i y y
D-247
US00001428
COASTAL BIOANALYSTS. INC. N PD E S ST A T IC ACLTTE/SALTWa T T R
(Form ACV48)
\EHIuert
Day1 1.0. 9 Liv*
0*r2 #Lrv .
Control 6.25
CI f
tO
C-2 -- 1 1 ____ -- L ____
II 1A .
/i
17 >6
tp
21 12.5 2-2 0 _
l.Q-- fo -
3-1 ' h
25 3-2 1 0
io .J
4-1 f P
-- LU--
50 4-2 f A
/
5-1 } .. _ J _ L _____
too
5-2 / o
n __ Li2_____
Obsorvrr G -tf
^ A ff
_
Tin: J 5 ____ i u r i \ _
F>r*i \ Survival
/ oo ( Oo
l O t Ob
1oo
i oo
C yprirtodon varieqatus
Sourc*:______
Hatchrurt/T 'irrr
\l
I **^2--
Acclimation wstac HWM ASW
O tlc-------------------------- S r f^ r y 3 - Q _
Acclnrotion T*rrp. E-a-g.)______ ( ^ . i? ----- -------- ^
~
fwdnjfnwtotru: *nm _2^2-- Vd 0t>*r------------------ ----
Elf ^ d r g dunng last: Not Iti
* " " --------------- 1*
0 tt* r ------------------
3 0 0 ni IroKm: 2 " < 0
SOOtrt H
2 "< H
ICOOrN
500
O O :-----------------
V
M * :-------------
liaoafmlorgarwnspar -attesta: t..-------- ^ "f*" " *----------- footopanod 1SI:BD j a 0thar._____ _ ftindoniauon lan^bu r ^
SET UP: r w . ( V / } % ) * 1 - T W A d * d _ l 2 i O --------------
Twr Aiwml Adfrd. 2 - a < _
.SrtUpBr. P O /c rK
INSTRUMENTATION USED;
Stani T heftiem aw N a t a : ^ I___ T S 0 .0 . M alar 1 (SNE8014475) 5 3 CP Digssnsa pH M atar (C95O05S3) sw nu.R 16C P B ianca [40020093) T S SCT 33 Matar |HS01S792)
SY S 0 .0 . M atar 2 (S491A02S329) C o n ati 240 |t t Matar (S 6 2 S 8 )
SP P o r t * M atar I8S2360) 3 ATC R aftactorm tar (A 3M )
0i>r
Orr 0
Or? 1
0*T 2
C :W
.? O . S '
N .y
1T
o r. u
i> -V
1 1 :5 1 -
E2 * ?r)-b M
y J9.5
3 ;fN
4 rO p . 3
5 < jy
5 S l > V A i T
/t> y _
C T -.f i'
1 ff. o V
7 -.7 1 #
2 r/5 C c
i?. > 6
T -.i?
pH )
- ;.n
4 g 'r ^ V
g ./f - T o?
5 f.a t
C t~9
t i-.f
20 .0 .
7 -A
O- ?9 -S r.j 7 .2 --
Irgli)
s
A L
3 T--<t 4 T -J
5 7-.h
C '$>
2 JA
?-S --
J7 S
.
P -.S J _ _
<P n . _ l n
^0 -
.-a r /
_ r 3 / i ____
N 3 O r>
3 h
_ s L Q -------
4 A
(Q / i
5 Jtt 1
- i .\
-Q f ) <9
7n _ ..
Intiah:
(rU
p ff- .. _ L i y _
N0TES:_---------------------- -------- -----------------------------------------------------------
r r o i t r * 1^
U. C 3 --------------- ------
&ty A utrw cK
As O uJiry A u r a a i oIH tar lor O st last I cartdy tKal rs> Uftbcw*
n a n s fffira Mcapla d pratocoI c a n a ra d d a n g rautffia irapactaa ( lass otlvrwiM ra d b ai .) I that tha dHa aasaat.I> fallaci th. rast it w a s parfonrad b C3J. Corrrmncs^
Je-
TEST i n - < T L f \ i t / li lf
D-248
s
US00001429
COASTAL BIOANALYSTS IN C EFFLUENT/DELUTION WATER DATA (Fom.EFDL0i?5)
IN ITIA L (A R R IV A L) E FFL U E N T C H A R A C TER ISTIC S:
Sample Date 1 1 1 / ( 7 / 4 ? 1
1
1 -
1
1
1
Temp. (C) 1 Y . O 1 f 1 1 1 1
Conduci. (uMHOS)
I II II |
Salinity (g/kg) 1 M
1
11
11
1
pH 1 % a ? i
i
11 I
Diss. Oxygen (mg/1) l ( M
1
11
11
1
Tot. Chlorine (mg/1)
1 11 11 1
NH3-N (mg/1) l l . f
1
1
1
11
1
Hard (mg/1 CaC03) 1 1 7 3 1
11
11
1
Alkln (mg/1 CaC03) | Q ^
|
f|
I1!
Color/Appearance1 1 .Y
1 e1
1
11
1
Initials
t-'w
1
11
11 1
1 C-Clear O-Opaque Y-Yellow B-Brown Bl-Black G-Green T-Tuibid SI -Solids (S-SIighi M-Moderate V-Very Much)
EFFLUENT ADJUSTMENT/POST-ADJUSTMENT MEASUREMENTS:
Date li/ Z / f / ^ l
III
Temp. (C) 1 ^ 6 . } 1 I I I
Salinity (g/kg) 1 3 0 1 I I I
D O. (mg/1) I 7 - . 1 I I I
Aeration Rate I -
1
I
I
I
Aeration Time (min 1 -- 1 I I I
Adjust. D O. (mg/1) 1 -
1
!
I
I
PH 1S- l % 1 I I I
Tot Chlorine (mg/1)
| III
Initials
JM 1
I
I
I
I I I I I I I I I I
ACUTE
I II I II I II I II I II I II I II I II I II I - II
DILUTION WATER CHARACTERISTICS:
DMW:
ACUTE
Date 11//(7^?I
II
I I II
Temp. (C) la 0.7- 1 I I
I I II
Conduct. (uMHOS) U / l I I I I I I II
Salinity (g/kg) 1 a o 1 I I
I I II
pH 1 7 -^ s - 1 I I I I I If
Diss. Oxygen (mg/1)
1I I I I
II
Hard (mg/1 CaC03) I a iA I i I
I1
1
Alkln (mg/1 CaC03) 1
1
I
I
I
I
1 II
Initials
1 Vrf I
I
I
I
I
1 II
EFFLUENT ID:
~1on
(\>'S_______ TEST ID: <5 & f t ^ ? f Ve/.
^.
D -249
US00001430
D-250
US00001431
ODU/Oceana Acute Effluent Toxicity Tests
M y s i d o p s i s b a h i a <i C y p r i n o d o n v a r i e g a t u s
Sample I D. Feed Contract No. N00187-93-D-9748 . * Delivery Order No. 0235 J.O. No. 1912290, Item No. 0002BJ, 0002BH, 0003AD
PWCNo. 1378 11/17/97 1100 Sample No. JRA97-13128
Submitted to:
Ms. Merrill Anderson-Ashcraft Navy Public Works Center Code 930, Building Z-140 Norfolk, VA 23511
By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062
(804) 642-0168
D-251
US00001432
O D U -O CEA N A Sam ple I D. Feed Slock 11:17/97 Sample 97-13128 Test ID: JRA97143
Coastal Bioanalysts Inc.
I. TEST RESULTS
% Effluent Concentration
0
6.3
12.5 25.0 50 0
100
48-h LC50: 95% Confidence Limits: Calculation Method:
Toxic Units (T U ):
Table 1. Summary Acute Test Results
Mean (%) Organism Survival
M . bahia
C. variegatus
2Lh 10
48-h 100
2 100
48-h 100
100 100 10 80
10 0 100 60
00
100 100 100 100 100 100 85 5
00
23.3% 18.4-29.5 ' Spearman-Karber
4.29
36.6% 34.2-39.2 Spearman-Karber
2.73
Acute Test Information:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
M . b a h i a Test 48-h Static Survival 11/18/97 1720 11/20/97 1620 CBI stock cultures 11/14/97 20 g/kg 19.2-20.8C Yes 11/18 1700
C v a r i e g a t u s Test 48-h Static Survival 11/18/97 1720 11/20/97 1730 Aquatic Bio Systems, Inc.
11/15/97 20 g/kg 19.2-20.8C Yes 11/18 1700
D-252
US00001433
O D U 'W E A N A S am pli I D. Feed S tock 11/17.97 Sam pli 9 7-3I28 T U D : JRA9714J
Coastal Bioanalysts Inc.
Sample Collection Pate/Time
11/17/97 1100
Sample Use Baies/Times
11/18/97 I720/17203
Aeration2 Rate/Time
n/a
Pretreatment/ Adjustment
HWMSalts
2Aeration often necessary to reduce sample oxygen concentration to 100% saturation after warming. 3.V/ bahia-'C. variegatus test time.
Table 2. Effluent and Dilution Water 4 Chemical & Physical Characteristics (Mean Value (Standard Deviation))
Parameter
Temperature (C)
Salinity (gAg)
Conductivity (uMHOS/cm)
PH
Dissolved Oxygen (mg/1)
Total Hardness (mg/1 as CaCOj)
Alkalinity (mg/1 as CaCO-j)
Total Chlorine (mg/1)
n h 3-n (mg/I)
Initial Effluent
2.3 (-)
N/A
1100 (-)
7.15 (-)
7.9 (-)
126 (-)
460 (-)
<D.L. (-)
29.0 (-)
Adjusted Effluent 20.8 (-) 22 (-) N/A
7.41 (-) 7.0 (~ ) N/A
N/A
N.D.
N.D.
Dilution W ater 20.7 (-) 20 (--)
. N/A
7.95 (-) 7.9 (--) N/A
N/A
N.D.
N.D.
4Efnncni parameters measured upon receipt of sample in lab and after adjustment on day of use. Dilution water (Wimex lnv-Mnrincmix in ASTM Type I deionized water) values measured after preparation of diluent on day of use.
D-253
US00001434
O D I'O C E A N A Sample ID . F eedS hxk 11 17-97 S am p le 97-13128 T est ID: JR A 9 7 I4 3
n . WATER QUALITY
Coastal Bioanalysts Inc.
Parameter Temperature
(C) DO. (mg/1) PH
Salinity (gAg)
Parameter Temperature
(Q DO. (mg/1) PH
Salinity (gAg)
Control
20.1 (0.6)
7.8 (0.1)
7.84 (0.09)
20 (0)
Table 3. Water Quality Summary
M . baha Test
Mean Value (Standard Deviation)
63%
123%
2 i%
50%
20.2 (0.6)
7.7 (0.2)
7.86 (0.06)
20 (0)
20.1 (0.8)
7.5 (0.5)
7.88 (0.20)
20 (0)
20.0 (0.8)
7.3 (0.6)
7.76 (0.29)
2! (0)
20.1 (0.8)
6.5 (18)
7.84 (0.40)
21 (0)
100%
20.6 (0.3)
5.6 (2.5)
7.74 (0.47)
22 (0)
.Control
20.1 (0.6)
7.8 (0.1)
7.84 (0.09)
20 (0)
C. variegatus Test
Mean Value (Standard Deviation)
6.3%
12.5%
25%
30%
20 1 (0.6)
7.7 (0.2)
7.84 (0.04)
20 1 (0.6)
7.3 (0.8)
7.85 (0.17)
20.0 (0.6)
H.
7.1 (1-1)
7.83 (0.36)
20.1 (0.6)
6.6 (1.7)
7.91 (0.46)
20 20 21 21 (0) (0) (0) (0)
100%
20.3 (0.7)
5.6 (2.8)
7.84 (0.60)
22 (0)
D-254
US00001435
G D I' OCEANA Sample I D. Feed Slock 1I.T7/97 Sample 97-13128 T e a ID: JRA97143
DI. OUTFALL DATA SUMMARY
j j j j Coastal Bioanalysts Inc.
Test Pate
9/30/97 11/3/97 11/18/97*
Table 4. Cumulative Data Summary - Acute & Chronic Tests (Tests conducted on this outfall by CBI)
Invertebrate
Acute Tests
Chronic Tests
LC50 T.U.
H PE C T.U.
8 .1 % 12.5% 23.3%
12.35 8. db 4.29
N/A N/A N/A N/A N/A N/A
Vertebrate
Acute Tests
Chronic Tests
LC50 T.U.
NOEC T.U.
3.5% 19.7% 36.6%
28.57 5.08 2.73
N/A N/A N/A N/A N/A N/A _ .
'Results submitted with this repon
IV. QUALITY ASSURANCE
M . b a h ia - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
C v a r ie g a t u s - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
CBI Stock Cultures 11/20/97-11/22/97 KC1 (Sigma "Ultra"Lot#76Hl039l) 343, 49.0, 700, 1000 mg/1 100% 574.8 mg/1 (532.7-620.0) 610.6 mg/1 (488.4-732.8)
Aquatic Bio Systems, Inc. (same batch as test)
11/18/97-11/20/97
'
KC1 (Sigma "Ultra" Lot#76H10391)
432, 720, 1200, 2000 mg/1
100%
1029.5 mg/1 (881.7-1202.0)
1079.5 mg/1 (896.5-1262.5)
D-255
US00001436
CUMULATIVE DATA SUMMARY NPDES Permit #: N/A Oceana - ODU Feed S.
I 2/12/97 <6^25^ V' ` "'
| 2/28/97 Invalid
| 3/12/97 17.7
3/zu/y/ iy.5
iu b i y/ 33
9/30/97 8T
11/3/97 12.5
11/18/97 23.3
:
31
52 |35 34 13.5 119.7 [36j6
.Virtbratr.^r- "-S NOEC%
:---------------^ = ============j
| [ 1f
1 1
|
3
Supplem ent
D-256
US00001437
COASTAL BIOANALYSTS. INC. NPDES STATIC ACUTE/SALTWATER
(Forni AMB48)
l Effluent
1.0.
Oayl f Live
0ay2
9 Lrv*
F inali Survival
C-l \ f C-2 h
10
) CO
1
M ip
_ L o _________
... co6 2 5 1-2 ! 0
'9 .,
;
2-1 t p
(
12.S 22 M
__ k ____
9,0
3-1
a
25
3-2 _ D _____ _Q _____
0
4-1 n
_____
50 42 I
<T
1 /6
s-l o 100 S-2 o
_ 0 _____ ______ L
Observer.
CH
t-n
Tirre: ' T i < - i * O n
Mysidobsis bahia___________
S c u r a :______CBI
Hatch D at^T iTM :. n m m --
A c d ira tio n w a te r HWM ASW
Othar^
Salinity: ' 2 - 0
AerlifTetion Terrp- (range):
[
Faadng pnor to test: Anania
3 ^ _______ ____ ~2^Xl % Jd Other__________
Feedi^ Airing lest: Not led D
Anetrie f ' - i d Other.
C hanter size: ^ 0 0 n i ^ Test voiu n : 2 0 0 n i J 0
500 iri
1000 iri
O ther
250 iri
5 0 0 rri
O tter
No. n o T c e ta r 1 --
ni ni
fte n p e n o rt 161:80 Q \ Other
fl kfanption tw reteta no. ^ " 2
SETUP. D f _ ! l j J U A D -- T m W . w A d * d _ l ^ t i y ________
Fin A rire b Adfrd I Q
S h Up &y
INSTRUMENTATION USED;
LStaro Tham m rat- Nuntec.
TS D-0. Meier I (SNES01447S) n C? Digserae |H Meter (09500663)
S enotiu R160P B e in e (40020093) T S SCT 33 Meter (H8016792)
TSI 0 .0 . M eter * 2 (SN91AC2S329)
8 Cerriep 2 4 0 pH M eter (SN52S*}
SP PortepH2 M eter (952360)
S p e m e ATC ReTteetarneter (A366)
Other
OeyO____ Day1____ 0y2
C SO 7 o.J iif.r
T 1 Q . 7 ^ (7 3 H . S '
E
ffl
? O o.ff a 0 .3
P 3 clP.l- c} n . 3
4 ^<3.f A o V / f . o
s Z o V Q -V c iS i . l f o
737?
7- 5- 7 . O 3 .? D
pH 2 ? . . o r
TL .*ti
3 1 . Y 3 h * 3 J . *1 V
4 7 3?
t, OD
s AVf
? .// --
c 7 -T
1 7, s
T-.rt
3_A _
0.0. 2 U . i
>-fl 7 -2 ? --
(mgEI) 3 M
7 -7
7 -U --
4 v .s -
7 -<
7 Ot--
s 3 , ^ 7 .y
sc
AI l
2
N3
4
5 Replicata9
On ac S (> <7/
1 Se
Initials:
Cm
NOTES-[jirL -U u
an 0 0 -- t^l (; _ 3 a x _
^ 4 ____ _3Lf_____ . a / ._ O f -
.7 0 -- -
0/
G f f -- t / A -,
7^.-. "t_
(/ .- X- ~ H.E. ,h^ /**r)f*,
Corxi . . 1 .
----- y-{ f l
-------------------
Ifc r r 1- J- V~y^ *" Tl O T.l
----ftfc. `l|,4j<9
TEST I D.: D-257
US00001438
TRIMMED SPEARMAN-KARBER METHOD. VERSION 1 .5 -
DATE:
1 1 /1 8 /9 7
TOXICANT : FEED STOCK
S P E C IE S : M BAHIA
TEST NUMBER: JR A 9 7 1 4 3
D U R A T IO N :
48 H
RAW D A T A : --------------------
C o n c e n tra tio n (% )
.0 0 6 .2 5 1 2 .5 0 2 5 .0 0 5 0 .0 0 1 0 0 .0 0
N um ber E xposed
20 20 20 20 20 20
M o rta litie
0 0 4 20 8 20
SPEARM AN-KARBER T R IM :
.0 0 %
S P E A R M A N - K A R B E R E S T I M A T E S 1:
LC50:
95% LOWER C O N F ID E N C E :
95% U PPER C O N FID E N C E :
2 3 .3 3 1 8 .4 2 2 9 .5 4 .
NOTE:
M ORTALITY PRO PO RTIO N S WERE NOT MONOTONICALLY IN C R E A S IN G . A D JU S T M E N T S W ERE MADE P R IO R TO S P E A R M A N -K A R B E R E S T IM A T IO N .
D-258
US00001439
C O A S T A L B IO A N A L Y S T S. IN C . N P D E S S T A T IC A C U T E /S A L T W A T E R
(FormACV48)
\ Effluent 6.25
1.0.
0*f1 t Irv*
Day 2 9 Uve
C- \V - J A ___
C-2 ! t f ____ - J A ____
M - M ___ -- 0 -- -
1-2 10
1A
fnal * Survival
[ ce
i l'O
-J JAA___12.5
2-1 1 0 2-2 1 0 -
_______ \ G
3-1 _ U 1 ____ / f t
i 00
25 3-2 1 1> J A ________
4-1 ' & ___ J ______
f.
50 4-2 M . _________ A
?
5-1
100 62
c>
0
Observer G /
Tire: l i s t s . }
i------------------- 1
Sdito:
Hatch DatartiTM: l U K l ' Y ?
Acdifiation Tenp.(wg|:
IO Z- O aK
Feeing dring test Not fed D Artemi
/d 0thr
C k an tarrtm : 303 ,W T n t n l u n : 2 0 0 rH
5 0 0 rH H
250 ni 0
lOOOrH SOOtrt
Q r____________ni
V-
O tE a r____________ n i
Intiil in . or&ritna sm i r i b n
1Q
-
-- --------
Pholopnnod 16L8D ^
OtEar._________ R anrbtratB n ta r a to ti no. ^J> ~ ^
SETUP; 0 I1 / n l < n . rumWMvAdcM l ^ b _
F in . A flirah AAW f ? - S t
Sat Up b f P Q / f a ' *
INSTRUMENTATION USED:
Statt) lE a rrro ra ta r I f a r h f .
1
T 9 0 .0 . M atte 1 1 (SNE8014475)
Q CP Diiaansa pH Malar {03500563}
Santana R160P M anca (40020093)
T 9 SCT 33 Malar [H8016792)
gT 9 0 .0 . Matar 1 2 (SN91AC26329) C om rq 240 (H Matar [9 6 2 6 3 )
SP P o m Matar I8S2360) { 3 S p a r t ATC Rafiactom atar (A 366)
Othar
OayO
D*y 1
Day 2
C 5>o.2 3 o / H . L
T
o .1 rt.fi
4 7
E2
ft f
/f 2
M
3 Xo.X I'M
/'t c r
4 a p .if
ft X
5 fi. ff
C ? .? 7
1
.S 7
2 %A
2 T - .b 'T
7 ft-
pH
3
. / o
7 .4 S -
4 Jr ~ J ) --
`B - i l
.S T l . V / 1? c i t o
C XJ
2.
2.1
1 X .s
0.0. 2 b.W
JJ \X 9
(ml) 3 S J
XX X to
X7
^2 < >r ---
5 3 S ~fc\ to
SC A , r4 / l
>6
7 /J\)
t l---<3 A
2 ' J Zi ~1 ___ g --
N 3-
A l ^ ____
(Vg) 4 / 2 /
--3S4--/t-------
5
Replicata 9
a
2 A ____ c3 A _____
Initiais:
C f K - 0-/T
lu
NOTES
/. >r
<X/ J-' -1s'J- 'i P-&X J j_a_
flt-ca J-.J,__y.
IXO
Quafity Assurance: As Qtafiry A s s u m e * officer for ts t e s t i ca m ly that no ^ r a ti o n s from ac eo p ttd protocol occurred daing reutino inspections ( t o s otherw ise t * d W ow ) and that tho (tots aecw atrty rofloct tho
tost as it w as prforTr*d bf C8I.
C orm vnts
W i n 11 P I 33-
TEST I D.: v /fc fl ^XtH 3
I
D-259
US00001440
TRIMMED SPEARMAN-KARBER METHOD. VERSION l.S
DATE: 11/18/97 TOXICANT : FEED STOCK SPECIES: C VARIEGATUS
TEST NUMBER: JRA97I43
DURATION-
RAW DATA: ---------
Concentration <*)
.00 6.25 12.50 25.00 50.00 100.00
Number Exposed
20 20 20 20 20 20
Mortalities
0 0 0 0 19 20
SPEARMAN-KARBER TRIM:
.00%
.SPEARMAN-KARBER ESTIMATES :
LC50:
95* LOWER CONFIDENCE:
95% UPPER CONFIDENCE:
36.60 34.21 39.16
48 H
COASTAL BIOANALYSTS NC
E F F L U E N T /D IL U T IO N W A T E R D A T A (Form EFDL0 1 9 3 )
INITIAL (ARRIVAL) EFFLUENT CHARACTERISTICS: --------------------------------- -- ------ ---------- --------
Sample Date l u / n / fi i 1__________ 1
Temp (C) l . 3 1
1
Conduct. (uMHOS) I l / O i ) 1
1
! "1
1
1 1
1
f
1
1
1
1
Salinity (g/kg) \t~ 1
1
1
1
11
1
PH U . I 5 - 1 1 1
11
Diss Oxygen (mg/I) 1 1 -
1
1
1
i
1
1
Tot. Chlorine (mg/1) 1 ' - o < - 1 1 1
11
NH3-N (mg/1) 1 & . 0 1 1 1 1 1
Hard (mg/1 C aC 03) 1 / 0 (n 1 1 1 1 1 1
Alkln (mg/1 CaC03) V o o 1 1 1 1 1 1
Color/Appearance* 0 6 1 1 1
1!
initials
<v(4
1 ^ ~ ,------- -- ,
1
1i
1
1
!
' C-CIear O-Opaque Y-YcUow B-Brown BI-BIack G-Green T-Turbid SI -Solids (S-Slight M-Modcralc V-Very Much)
EFFLUENT ADJUSTMENT/POST-ADJUSTMENT MEASUREMENTS:
ACUTE
Date lf///?/47M
1 1 I I I II
Temp (C) ISo.1t I I I I I I II Salinity (g/kg) l3-> I I I I I I II
D O (mg/1) h e I I I I I I II
Aeration Rate I -
I I
I
I
I
I
II
Aeration Time (min) j ---- | j | I I I II
Adjust. D O. (mg/1) I --
i
I
I
I
I
I
II
pH n . V f i l i I I I II
Tot Chlorine (mg/1)
| | | . I I I II
Initials
I ( r t f -----I--------------i-------- ------I--------------I------ --,----I--------------I--------------II-------------
DILUTION WATER CHARACTERISTICS: DMW:
ACUTE
Date lll/IS /47 I I I I ` I I II
Temp. (C) I'w.7 I I I I I I H
Conduct. (uMHOS) \ / v f
I
|
|
I > I I II
Salinity (g/kg) i r i I I I I I I II
pH I7.< i I I I I I If Diss Oxygen (mg/1) I ? . ? I I I I I I II
Hard (mg/1 CaC03) I A/l9 I I I I I
II
Alk!n (mg/1 CaC03) I A//? I I I I I I n \
Initials
I C-lf- I
I
I
I
I
I
li
EFFLUENT ID V< x C? 5 4 PC. t __________ TEST ID: 7 f l & V I Y 3 '
D-260
US00001441
N0U-19-1SS7 13=09
JRR
Toxicity Test Sample Chain of Custody (Please complete all information)
Facility. Q \ d X lr iV u o n U n n fp rQ itij.
Hr,. Q c x p o l y : .
33.613*3
757 673 1456 P . 11
County.
Pipc/Outfall/Location,
N'PDES#
Insercam Waste Cone
Sample collected by (print^ignV jJo^O yi
Affiliation OPU \?tW-
7-
Tvpc of sample ^
(Grab):
Date
(Composite): From Date.
To Date__
-- Time-- \VOOA**V Time ~r.
-- Tune.
Subsamples comprising composite: *-/o^ Number___________ Frequency of collection-------------. Volume.
'Temperature of sample in sample collection device-- O Q_
Final temperature of cfQucnt at sample collection point, <?<? ^ .C /
'Is sample collection device chilled? ^C-S Is sample packed on ice for s b ip m e a t? _ ^ S _
'It is required that all samples remain at 0 - 4C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency (Do not freeze!)
Is the sample chlorinated?
dechlormated?_*L If so, how?-------- -------------- --
Permit with interim chlorine limit? ~ __ ll yes - limit (mg/L)------- ------------- ---------
F ield p tr ~7.
Field Total R esidual Chlorine--------- -- --
' Comments/Samplc description..
---------------------------------;--------- -------
Tvpc of test(s) to be performed M ^ l ( j ^V'Cirfl p ;--dO jpT l n c d O (i
(Specify organisms)
&rv
Method of shipmenr_J2sJiVer^_b*- O Q A ---------
.-- _ ----------------
Print a Sign Names
1 . hy J e w f e r J O S Date \ ' / \ * / 5 L Time. \ Q ' C Q a r t
P^rrlvpH hv / . j f o z / x p a , ' y > ~ t 0 a . . Date
Time_^lOj$Zl___ 7
Relinquished by $ i ^ h ^ t - P P j ,-- dDamte,j jI h1/ t m<7~7. Time !
Received bv.
_____ Date
. Tme_Z/. ^ - -, . M m*:
FOR REED LAB USE ONLY
J R A * J 2 zlll ? L
Arrival Temperature_______ _
Color_____ -
' Odor_______________ .
pH--------- DO (mg/L)_
Conductivity Qunhos/cm).
Salinity (ppt).
TRC (mg/L). ___ Method _____
On ice?. Solids.
D-261
5/li/1*
US00001442
V( NO
(N
US00001443
Toxicity Test Sample Chain of Custody (Please complete all information)
Fr-.-i:ry r>\< D o v n i/ito n U nW -prQ ilv)----- :-------
A ddress. iO o *'
V'A-
I
County.
P ip e/O utfall/L ocation
NN PPDDEESSs2--------- -----------------------------------------I-n--s--t-r-e-a--m----W---a-s-t-e---C---o-n--e------------------ ------------------------,
Sam ple'coliected by ( p d n . & s i g n l M n ^ & x A g ilia .jo .O PU f r w . E p g . l q b
T_ ype o- f, s_a_m__p_l_e /^ <(Gmr,,ahbV):
n-airiee 7 n / V _ T im ee _ %P ; ' f r Vf f'i l..
~~ (C om posite): From D ate__________ T im e--------------
To D ate_______~~ Tim e-------T _
Subsamples com prising composite:
Mnmher
Frequency of collection.
V olum e.
'T em perature of sam ple in sam ple collection d e v ic e . ^ O 0 ?
Final temperature of effluent at sample collection point
^
'Is sample collection device chilled?^iiS_ Is sample packed on ice for shipment?,
'I t is re q u ire d th a t all sam p les re m a in a t 0 - 4C d u rin g collection perio d an d sh ip m en t for
data to be accepted by the a p p ro p ria te R egulatory Agency (Do not freeze!)
Is the sam ple chlorinated?. I f ____ d ec h lo rin ated ? _ 2 _ If so, how ?--------- = _ -----------------
Perm it w ith interim chlorine lim it?------- Z ----- U >'es " ljmlt
-------------------------------- '
Field oH
Field Total Residual Chlorine---------------- --
Commenis/Sample d ^ rip .io n . M - P O n W . ( *
-----------------------
T vpe o f tcst(s) 10 be perform ed----- C \ o n 't t f p , -- C - C U p n n o r
(Specify organisms) _ -- V tA fr
---------------------------------- -
Method o f s h i p m e n t
^ Cfyik
Print & Sisn Names
Relinquished - y J e v . r r . f b r -JO S O * - Buie " /
/J f ir ttii
D a te J l|im i
Relinquished Received by.
Mal.
Date /<
Ti-e \Q-CC<*1 TimeM S a ___ J t 1 5 7 /
Time / / > ! _ Time f / l - O ---------
FOR REED LAB USE ONLY
JRA #.
A r r iv a l T e m p e r a c u r e _ -----------------
Color
Odor___________ ._______
PH _
____ DO (m z/L )________ C onductivity (um hos/cm )_
Salinity (pot)________ T R C (m g/L )----------- M ethod----------------
On ice?. . -Solids --
,@____ *c
D-263
.icv =/ : / ?
US00001444
Toxicity Test Sample Chain of Custody (Please complete all information)
Facility. 0 \ c D C v v ii> h O n , \ A m si'A' r Q it M Address. K) Q r ^ \ \ c , v/A- .4? 3 n , ^ l
I
County.
Pipe/Outfall/Location .
NPDESfi
[nstream Waste Cone
Sample collected by fprint&signV VJc^Qlfl
Affiliation
fcfW- Hn G .. \M ) o
T y p e o f sample ^ (Grab):
Date ^ / M / 0 ^7 Time 6t>''3D0 'P^vA.
~~ (Composite): From Date ~
Time --
To Date______ n _ Time --
Subsamples comprising composite:
Number________ Frequency of collection__________
Volume
'Temcerature of sample in sample collection device 0
Final temperature of effluent at sample collection point -Q Cf
'Is sample collection device chilled0
Is sample packed on ice for shipment?
`It is required that all samples remain at 0 - 4C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency fDo not freeze!)
ts the sample chlorinated?
dechlorinated? ^ [f so. how?___^5:__________ _
Permit with interim chlorine limit? ~ If yes - limit (mgdL)______ II-------------------
Field nH 7 .& ^
Field Total Residual Chlorine___________
Commems/Sample description A Q. ~
f?_hi._--------------------- -------------- -
Type of test(s) to be performed
^ViCtril p - CCtJpV~l
(Specify organisms)
VtTO 6 /^ A jlA o ____________________ .
Method of shipment P&Uv
(/CM.
. Print Sc Sisn Names
I. Relinquished hv
-0 0 5 0 * 0 Date 1^ / 1 %
Time \G -G Q rO
Received by_
Date H j t f k n
Time
37^
Relinquished b 4
Date t l ' / ( % f o n
Time / / 3-4)_____
Received bv.
Date l i f l ?/*?~7 Time //2 -0 ______
FOR REED LAB USE ONLY JRA."________ ;_________ _ Arrival Temperature____________ On ice?________ Color.______________________ __ Odor________________________ Solids-----pH__________ DO (mg/L)_______ Conductivity (umhos/cm)_______ @______C Salinity (ppt)_______ TRC (mg/L)______ Method____________________ _
D-264
.lev r/Si/T-*
US00001445
Toxicity Test Sample Chain of Custody (Please complete all information)
Facility Address.
O l JL
lO O f
POVYhyO ttOyri
n Ik . '/A t 4?
(.ni^
3 ^pC
r l
P
r
Q
i
H
County__ NPDES?
Pipe/Outfall/Location Instream Waste Cone
Sample collected by (prim&si.gn)J J o S C n # 6 ^
Type of sample , S (Grab):
Date 1' / n / ^ P
Affiliation ODU & W . V ^ r x LqU
Time (j s V p ry
^
__H-- (Composite): From Date ~
Time --
To Date_____ Subsamples comprising composite:
Time --
Number-------------_--- Frequency of collection_________ Volume__________ Temperature of sample in sample collection device O
Final temperature of effluent at sample collection point e&?. g C y
Ts sample collection device chilled?
Is sample packed on ice for shipment? 1-16$
It is required that all samples remain at 0 - 4C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency fDo not freeze!)
Is the sample chlorinated?, ^
cechlorlnated0
If so, how? V c c ___________
Permit with interim chlorine limit? ~ If yes - limit fmg/L)
~~____________
Field pH ~ 7-C\ ^ -----
Field Total Residual Chlorine___________
Commencs/Sample description
- ( V ) A V<>-0 V .2 . V ir C L & r & ^ O f ] ________________
Type of test(s) to be performed
(Specify organisms)
\/a f \
Method of shipment
^ C/Cll
-.Km? p , C c U ^ n ' n C d o f ) `^
Print & Sisn Names
I. Relinquished bv
. Q S Q t i Date ^ / ^
Time___\_G__'-_G_ OarO
Received bv.
t ^ j Q j > o Date i t l l ^ k n
Time
TZ)
Relinquished bv
23Received bv
O f f Date Date i l f i z h - y
Tune / f Time //3 .Q
JRArr.
FOR REED LAB USE ONLY Arrival Temperature__________
Color.
' - Odor
PH_
DO (mg/L)_______ Conductivity (umhos/cm)_
Salinity (ppt). _____ TRC (mg/L)______ Method_________
On ice?. . Solids. ____
T
D-265
=/:5/?-
US00001446
Toxicity Test Sample Chain of Custody (Please complete all information)
F,rq:-y- X y j C l iC :r ;;? 1,,; ,vi
Address tv^ O 'r -Cr>V\c' V A c 0
Countv NPDES*_ ,, --- -------------- ----- -----------
Pipe/Outfall/Location instream Waste Cone
' P f " "'P1' - -- (G ^ ):
Da, e _ J V I 7 / 3 I _ Time g - f l O p m
--------- (Composite): From Date ~
Time -I
"
To Date-------- Time Subsamples comprising composite:
--_____ ~~
J ------
Number------------ .----- Frequency of collection_________ Volume
Temperature of sample in sample collection device ~ Q 0 /
~
Final temperature of effluent at sample collection point L 2 . t r ,
IS M m p Ie p a c te d on iM fo r a ;
2
required (hut ell samples remain ,, 0 - 4-C during collection period a,,d sh i^ ^ 71to7
a o e accepted bv the appropriate Regulatory Age.ncv (Do not freeze')
Is .he sample chlorinated?^
_ de=hlorina.ed?_to ,f s0 ho,, , y
Perm,, w,,h m.enm chlorine limir?------ _ _ lf y a . |imj, (m
I T ------------------
F i e l d p H ------ Z 1 0 _
FWHTrvni
~
w
Method of shipment
/ _____ y u v. i i \
bej QCU
Print &. Sign Names 1- Relinquished by__-jg*lri.ffe.v / 0 5 o ^
ft/ff/q 7
Received byJ i
------ D
Relinquished Received bv_
Date--y / ff/ q j Date_j/ //? /< ? 7
^ --C O *)
Time / / > > Time / / i n
JRA_
Color, pH __ Salinity (pot)
FOR REED LAB USE ONLY
Arrival Temperature____ _______ __ _ Odor DO (mg/L) ------- Conductivity (umhos/cm)_ TRC (mg/L)______ Method,____
On ice? . Solids____ .. C
D-266
3ev :/ 16/r-i
US00001447
Toxicity Test Sample Chain of Custody (Please complete ail information)
Facility_ Q \ c D C v n i / i i O n . U r n ^ r Q i f t y f
A. d.d.re s s .
.vf/AA - ^
^
si
Countv
Pipe/Outfail/Location .
NPDES
Instream Waste Cone
Sample'collected by (print&siml-jJ o ^ O n / ~ ^ Q ^ Affiliation ODU Vw.
Type of sample. / .(Grab):
Date V 17
Time. JIAO
LqU T
.(Composite): From Date.
Time.
To Dare Subsamples comprising composite:
Time
Number________ Frequency of colIcction_____________ Volume.
'Temperature of sample in sample collection device ^ 0
Final temperature of effluent at sample collection point
*Cs
s sample collection device chilled?
Is sample packed on ice for shipment? vi6S .
It is required that all samples remain at 0 - 4C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency fDo not freeze!)
Is the sample chlorinated? ^
cechlorinated?
If so, how?
___________
Permit with interim chlorine limit7 ~~ [f ves limit (mg/L)_
Field pH__Q O
Field Total Residual Chlorine.
Comments/Sample description & Q ' (sO
A F j^ p
m f o n 's YJiappnir
Type of test(s) to be performed
<AV>r\rfl p . C r t j p n 'n C c lo r)
(Specify organisms)
\ f c k ' f \ S'tyiV fuS ________i____________
Method of shipment Q-SUn/|5<'^ bej C V \A
Print & Sisn Names
1. Relinquished bv
..j q s C ^ Date \ \ / i % / A ~ 7 Time. \Q - C O a r O
Received bv.
Date ' l l Time. ^ .S > .-- # t n r
Relinquished bv 7 f c d l f j ! , ~ X ^ < 2 Q Z Dare ( f / l j / c n
Received bv
Date // //? / ? - )
Time. Time. 1 / 7 , 0
FOR REED LAB USE ONLY
JR_A.nr_
Arrival Temperature__________
Color,
Odor_______ ________
PH _
DO (mg/L)_______ Conductivity (umhos/cm).
Salinity (ppt). _____ TRC (mg/L)______ Method_________
On ice? . Sofids. . @____
D-267
1er z/lifii
US00001448
Toxicity Test Sample Chain of Custody (Please comolete all information)
Facility, Q l c l7C vnivO tr;yi. U n i-.f-crO i tuf
A. d.d. ress.
ti .FA
J
,,REED . V^ci?C
Countv, NPDES#.
Pipe/Outfall/Location [nstream Waste Cone
Sample'collected by (print&si.mklJ c ^ O n
Affiliation 0D \I \EVw. Tn /? Ld?U
Type of sample ^ (Grab):
Date r,/ 1 7 / 6H Time 7: 30
~~ (Composite): From Date ~ Time --_______'
To Date______-- Time --
Subsamples comprising composite:
Number______ Frequency of collection. `Temperature of sample in sample collection device 0 (v
Volume
Final temperature of effluent at sample collection point
`Is
^
sample
collection
device
chilled?
s_
Is sample packed on ice for ship' ment? U&S
It is required that all samples remain at 0 --4C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency (Do not freeze!)
Is the sample chlorinated? ^
dechlorinated? ^ [f so. how? 'TV.____________
Permit with interim chlorine limit? ~ if yes - limit (rns/L)______ Cl_____________
Field pH___Field Total Residual Chlorine_________________________
Comments/Sample description
(oO ypriri.
ri f e n f C h l S ^ J U X ^ j y i \ r
Type of test(s) to be performed
^Vinrfl p . C r U p f t 'nOcl.Or')
(Specify organisms) Method of shipment
VcUf CCW.
u9
--
- Print & Sisn Names
I. . Relinquished bv
, j 0 3 O f ^ Date
Received bv
0 JP<? > Date.b..l..tr/--t % ( 19 f7
2. Relinquished bv A J b & L u ,
Date i f ! i ^ h l
Received bv
-iL c ^ _
Q
Date t / f i Z h l
Time \Q-GQarO
Time Q.S3 Tune / ( X Time J/X^Ct
FOR REED LAB USE ONLY
JRArr,
Arrival Temperature__________
Color,
Odor______________
PH _
DO (mg/L)_______ Conductivity (timhos/cm),
Salinity (ppt). _____ TRC (mg/L)______ Method__________
On ice? .Solids,
@_____
D-26S
3e*'
US00001449
Toxicity Test Sample Chain of Costodi (Please complete all information)
Facility Q \ d L Y ) Q V Y \ \ Y ) \ Q r ) . U y \ \ fA'y'Q ; \ \ A
Address.
TT
County____
N'PDESr
-------- ti
Pipe/Outfall/Location [nstream Waaoslt'e VC--UoUnCe
Sample collected by (print&si.gn)J J p ^ O n
Affiliation^ P i) Vw. rin 47 L U
Type of sample...^ (Grab):
Date V <7/v H Time \ 0 ^ LV^~ n\........... 3
_(Composire): From Date. To Date
Subsamples comprising composite:
Time. Time
Number-------- ..
Frequency of co!lection_
Temperature of sample in sample collection device ^ O
Volume
Final temperature of effluent at sample collection point
Is sample collection device chilIed?jjfrS Is sample packed on ice for shipment?
It is required that all samples remain at 0 - 43C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency (Do not freeze!)
Is the sample chlorinated?__ D _ dechlorinated?
If so. how? " A - ___________
Permit with interim chlorine limit? -
[f ves - limit (ms/L)
Field oK % . S 3 -
Field Total Residual Chlorine
Comments/Sample description M iYO cl L t ^ n p r ffrerA
--
ltt> RjLP{Ql9r')
Type of test(s) to be performed (Specify organisms)
Method of shipment pglNgriij
PkjrriC? S b r in i p , P .r y p n n C 'c io n
V /f lT `
^
(yC\A
Print & Sisn Names
I. Relinquished bv v7e*irtife.- JCSQA^ Date \X/Y l/ C I Time \ 0 ~U C W O
Received
Date f r / f t f r ~ 7
Time C jS d
4 /? ? p
Relinquished bv.
( h ^ d Q j Z , Date f i l i a l
Time I f
Received bv
Date V / r / 9 7
Time i 12.0
FOR REED LAB USE ONLY
JRA*.
Arrival Temperature__________
Color.
. ~ Odor___________
pH--
DO (mg/L)_______ Conductivity (umhos/cm)_
Salinity (ppt). -------- TRC (mg/L)______ Method_________
On ice? . Solids____ _____ C
D-269
Xe-r z r . i f ' i
US00001450
Facility Address.
Toxicity Test Sample Chain of Custody (Please complete all information!
County. NPDES*
--------
Pipe/Outfall/Location Insircam Wr* UaOstUew CI^UoInIUe _______
Sample collected by (print^ignU j c S O n
A ffilia ^
Type of samppllee_Sj_--(Grab):
DDaattee `>/VJ 717A/'cni~7 tTiTMm.e 1\\:\r;>ODO/Aw>,n
.(Composite): From Date.
Time
To Date
Time --
Subsamples comprising composite: *y^
I^ ^ ^ J
Number---------- _I----- Frequency of collection_________ Volume Temperature of sample in sample collection device O
Final temperature of effluent at sample collection point
,
'Is sample collection device chilled'^ S _ Is sample packed on ice for shipment? 'i/j&S
It is-required that all samples remain at 0 - 4C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency (Do not freeze.')
Ts the sample chlorinated?,, ^
dechlorlnated?
If so, how? V a c ___________
Permit with interim chlorine limit? -
; yes - limit (mg/L)
--~_________
Field pH-- 7- ^0?--- '
Field Total Residual Chlorine________
Comments/Sample description
Q \O c \Z .
Tyoe of test(s) to be performed___p . ( V u p n n O J o f l (Specify organisms) \ f & ? g
Method of shipment PettVertj C PU
I- Relinquished bv Received by--
Print & Sign Names JO SQ /^ Date eU /lg /< T 7 ' Dat e
2. Relinquished by M
' I b U f Q f o ,, Patz ( t f M O y
ReC=ivcd by
_____ Date / / f / & 9 7
Tim. \C-GOar0
Time
$2l
Time__f__._M__ .
S3??
Time / / }co
JRA2__________________ Color____________ __
FOR REED LAB USE ONLY Arrival Temperature__________
-Odor________
---- :-------- - DO (mgT.)_______ _ Conductivity (umhos/cm)_
Salinity (ppi)_______ TRC (mg/L)______ Method_________
On ice? . Solids.
@_____ C
D-270
US00001451
unA iiM -ur-uuo i u u r n c u u n u
L L Ib lN I IINI-UrtIVtA IIU IN
L ^ Z33r E N V IR O N M E N T A L
& ANALYSIS REQUEST FORM
PWC ENVIRONMENTAL LABORATORY CODE 9 3 0 BLDG Z - 140 9742 MARYLAND AVENUE NORFOLK, VA 23511 - 3 0 9 5 PH: 1757)445-8851 FAX: 1757)445-8852
COMPANY/CQMMAND: ) (
CONTACT: fc o u a V o *
PHONE:
EXTL
J 0 -#: H 0 - ^ . 0 -
SIGNATURE: ^ ^
PERMIT NO.:
^ r\< ~
COPE'-
FAX:
LAB USEONLY *LINE ITEM SAMPLE NO SAMPLE IO/LOCATION
UMfU 1AACH
OH DATE
AT n t* SAMPUD TIHE 8T
m i k
.
1
t-oo
J.^ 7 1 (R lrX 1
!1 ^ ....f
(^7Slilt? 1i ' l l
\A - C i D h t i v 1
A " c X CfrA^n
(-V ~
^ ^ n v4-Vfcl
A- a fiilS C
n
MI,* " O..inS..^Mr4rW
1a . RUYm J L e tte r
STMT 1/ n | n STOP SUM
stop
STMT STOP -STMT HOP STMT J. STOP STMT
L S I STMI j
4O K * I
Qny M n
77'
rt.V )
1
Tr
lP lS -4
MATRIX . PRESERVATIVE
Ll
\
n
'
:
# Of SAHPUS /CONTAINERS
ANALYSIS
FIELD READINGS
preservation
pH TEMPERATURE OTHEA VEWTIEOAT
314. <...& i. . - S .L .....................
1 f J llz .
\ I 9 9 - / )IJ> )(4T,....... ................................... - ......... 9 7 - /
1
I - <3/J'Y \ <=n -.... - ................. ..... - ..................
______ ( _
_L _L
ct i - "blL o
.........................i ........ "" 7 7 9 7 - f i ) 5 7
J.
TYPE G -G R A B CF - COMPOSITE, FLOW CT - COMPOSITE, TIME
,--
WIHA,H0UHD (O tri):
COMMENTS:
L - LIQUID
S W - SWIRE
S SOLID
GS-G AS
S S -S E M I SOLID
,-v;.:;v9ONTffi[V Y
P PLASTIC'|i - ; B BAG-'.,' ;'' ' V- , A - AMBER(gi*) GL -GLASS C . CARTRIDGE. T TEFLON s 'r-.T t'i TEFLO jj L|HED LID * V . VOA 'Yh *'HEXANE RINSED
(FOR RUSH TURNAROUND STATEREASON BELOW)
R EG ULATIO N A P P L IE D :""
RCRA ( )
HRSD ( )
SDWA ( )
TSCA ( )
1 - COOL TO 45C HCL, pH<2
2 - H N03, pH<26 -0.006% Na2S203, 4 C
3 . H2S04, pH<2
7 - FIELD FILTER
4 - NaOH, pH>12
8 NONE
SAMPLING/COLLECTION CHARGE: POSSIBLE SAMPLE HAZARDS: COMMENTS:
CWA ( ) PHOTO ( )
SAMPLE DISPOSAL: ( 1JRETURN TO CLIENT ( )DISPOSAL BY LAB
CAA ( )
OTHER ( )
B.fl. NUMBER:............"....
CONTRACT LAB:
INIT.: DATE:
1 CUSTOMER IS RESPONSIBLE FOR ALL CHARGES NECESSARY FOR THE PROCESSING A N D A N A L YSIS O F SAM PLEISI. ALL RUSH SAMPLES ARE SUBJECT TO SURCHARGE.
CONTRACT NO.(S):
-mmmmmmmmmm 'om axcvsE vm y-
7. SAM PLES RECEIVED AFTER 2 :0 0 P M M O N . THURS. A N D 1 :0 0 P M ON FRID Y WILL BE 'PR O C ESSEO 'TH E N E X T BU SINESS DA Y 17:00 A M - 3 :3 0 PM).
<'FOR LAB USE ONLYII
REJECTED ( )
REASON:
[VlKLlHUUISHbU UT
4 prn
iPULUHJUISHtU HY;
M yj
saMpLes:uerfiC|At|n
HOLDING TIME( )
CONTAINERS( )
f a t . . "litbCF'U'EBYm!
jriEC'ITBY^g1 1m
IMPROPERLY SAMPLED( ) OTHER( )
CUMPANYICUMMANUT YO ) <' S iZ J L JCUMPANVY/UUMMANU: 6 / HT iCc ?V
c m h An / m m a n u : " V jX
CUMPANYTCaraMTTCr--UTT
INITIAL:
DATE:
" uA iiiM b: t i n h i 7 M
UAIbMIMh:/7 //^ )9 ?/A'7TI
' UU AA TIht/tIllIMMLh7Mi .?Rm
nXc
IQ ~
US00001452
D-272
US00001453
A PPEN D IX D-5 BNR Inhibition Pass-Through Acute Toxicity Test Results
120 ppm A F F F P re tre a te d w ith Fenton's R eagent
8
D-273'
US00001454
D-274
US00001455
Coastal Bioanalysts Inc.
ODU/Oceana Acute Effluent Toxicity Tests
M y sid o p sis bah ia & C yprin odon va rieg a tu s
Sample I.D. B3 Contract No. N00187-93-D-9748
s Delivery Order No. 0235 J.O. No. 1912290, Item No. 0002BJ, 0002BH
PWCNo. 1394 12/1/97 1725 Sample No. JRA97-13676
Submitted to: Ms. Merrill Anderson-Ashcraft
Navy Public Works Center Code 930, Building Z-140
Norfolk, VA 235II
By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062
(804) 642-0168
D-275
US00001456
ODC.OCEANA Sample l.D. B3 1Z 1 97 Sample 97-13676 Test ID: JRA 97I66
Coastal Bioanalysts Inc.
I. TEST RESULTS
% Effluent
Concentration
0
6.3
12.5
25.0
50.0
100
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acute Test Results
Mean (%) Organism Survival
M. baha
C. variegatus
24-h
48-h
24-h 4Sdh
106' 100
100 100
100 100
100 100
100 100
100 100
100 100 100 90 100 0
100 100 100 100 100 100
66.0% 60.1-72.4 Spearman-Karber
1.52
>100% N/A N/A <1.00
Acute Test Information:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
M . b a h ia Test
48-h Static
*
. Survival
12/2/97 1740
12/4/97 1700
Chesapeake Cultures, Inc.
12/2/97
20 g/kg
19.0-20.7C
No
N/A
C v a r i e g a t u s Test 48-h Static Survival 12/2/97 1740 12/4/97 1705 Aquatic Bio Systems, Inc. 11/30/97 20 g/kg 19.0-20.7C No N/A -
1Aeration necessary when D.O. concentration <4.0 mg/1.
D-276
US00001457
O D I 'OCEANA Sample I.D. B3 121.-97 Sample 97-13676 Ten ID: JRA97166
Coastal Bioanalysts Inc.
Sample Collection Date/Time
12/1/97 1725
Sample Use
Dates/Times
12/2/97 1740/17403
Aeration2 Rate/Time
n/a
Pretreatment/
Adjustment
HWM Salts
2Aeration often necessaiy to reduce sample oxygen concentration to 100% saturation after -warming. 3_y bahia/'C. variegatus test time.
Table 2. Effluent and Dilution Water 4 Chemical & Physical Characteristics (Mean Value (Standard Deviation))
i Parameter
Temperature (C)
Salinity (gAg)
Conductivity (uMHOS/cm)
pH
Dissolved Oxygen (mg/1)
Total Hardness (mg/1 as CaC03)
Alkalinity (mg/1 as CaC03)
Total Chlorine (mg/l)
n h 3-n (mg/1)
Initial Effluent
3.2
(...)
2 (...)
2680
(...)
8.17 . (--)
12.9 (-)
1 211 (--)
439 (-)
<D.L. (._)
'
2.6 (-)
Adjusted Effluent 20.5
(...)
20
(...)
N/A
8.00 (-) 7.9 (--) ' ' rN/A
N/A
N.D.
N.D.
Dilution W ater 20.1 (--) 20 (-) N/A
8.24 (--) 7.9 (--) N/A
N/A
N.D.
N.D. -.
4Efflucnt parameters measured upon receipt of sample in lab and after adjustment on day of use. Dilution water (Wimex hw-Marinemix in ASTM Type I deionized water) values measured after preparation of diluent on day of use.
D-277
US00001458
ODI* OCEANA Sample l.D. B3 IZ '-97 Sample 97-13676 Tesi ID: JR A 97166
Coastal Bioanalysts Inc:
O. WATER QUALITY
Parameter
Temperature (C)
DO.
(mg/1)
PH
Salinity (g/kg)
-
Control
20.4 (0.3)
7.9 (0.1)
8.14 (0.09)
21 (1)
Table 3. Water Quality Summary
M . baha Test
Mean Value (Standard Deviation)
6 *3%
20.4 (0.3)
7.9 (0)
8.15 (0.08)
21 0)
12.5%
20.4 (0.2)
7.9 (0)
8.16 (0.05)
21 0)
25%
20.4 (0.1)
7.9 (0.1)
8.16 (0.02)
20 (0)
50%
20.5 (0.2)
7.9 (0.1)
8.15 (0.07)
20 (0)
100%
20.5 (0.1)
7.9 (0.1)
8.13
(Oil)
20 (0)
Parameter
Temperature (C)
DO. (mg/1)
pH
Salinity (g/kg)
Control
20.5 (0.4)
7.9 . (0)
8.17 (0.07)
21 (1)
C. variegaius Test
Mean Value (Standard Deviation)
6 3%
20.5 (0.4)
7.9 (0)
8.18 (0.07)
21 (1)
12.5%
20.5 (0.4)
7.9 (0.1)
8.17 (0.04)
20 (0)
25%
20.6 =t0.4)
7.9 (0)
8.16 (0.01)
20 (0)
50%
20.6 (0.3)
7.9 (0-1)
8.15 (0.07)
20 (0)
100%
20.6 (0.3)
7.9 (0.1)
8.12 (0.11)
20 (0)
D-278
US00001459
ODU'OCKA-N'A Sample l.D. B3 111-97 Sample 97-13676 Test ID: JRA97166
Coastal Bioanalysts Inc.
IH. OUTFALL DATA SUMMARY
Test Pate
9/30/97 11/3/97 11/25/97 12/2/97*
Table 4. Cumulative Data Summary - Acute & Chronic Tests (Tests conducted on this outfall by CBI)
Invertebrate
Acute Tests LC50 T.U.
C b rO D ic Tests
NQEC UL
65.0% 45.0%
Jt 1.54 2.22
69.1% 66.0%
1.45 1.52
yrv; a
N/A N/A N/A N/A N/A N/A N/A N/A
Vertebrate
Acute Tests LC50 T.U.
Chronic Tests
NQE T.U.
>100% >100% >100% >100%
<1.00 <1.00 <1.00 <1.00
N/A N/A r N/A N/A
N/A, N/AN/A N/A
Results submined with this report
IV. QUALITY ASSURANCE
M . b a h i a - Acute
Reference Test Dates:
Reference Toxicant:
Test Concentrations:
Control Survival:
-v' '
LC50 Reference test (95% C.L.):
Control Chart LC50 (95% C.L.):
C v a r i e g a t u s - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
Chesapeake Cultures, Inc. (same batch as test) 12/2/97-12/4/97 KC1 (Sigma "Ultra" Lot#76H 10391) 343,490, 700, 1000 mg/1 100% 606.9 mg/1 (578.6-636.7) 608.2 mg/1 (489.3-727.0)
Aquatic Bio Systems, Inc. (same batch as test) 12/2/97-12/4/97 KC1 (Sigma "Ultra" Lot#76H10391) 432, 720, 1200, 2000 mg/1 100% 1169.7 mg/1 (1044.1-1310.5) 1074 9 mg/1 (890.8-1259.0) -
) D-279
US00001460
Date o f
2/12/97 >ioo 2/28/97 Invalid 3/12/97 >100 3/26/97 >100 9/30/97 65 11/3/97 45.0 11/18/97 75.2 11/25/97 69.1 12/2/97 66.0
CUMULATIVE DATA SUMMARY NPDES Permit #: N/A Oceana - ODU B-3
>100 >100. >100 >100 >100 >100 >100 >100 >100
Invertebrate/;. -,.1
Vertebrate^^;-^5^j NOC% " -;,;c
Simplement D-280
t
US00001461
npdes static acute/saltwater
COAST/U- BIOANALYSTS. INC.
X EtHm* Control
6 . 12-S 25 50 100
fO n 1
1.0. Dv*
C*l 1R C-2 lo 1-1 l(D 1-2 10
On 2 Uv
ib
\V
____ ---------
Fvri*
St/vnri
16Ci inn
2-1 P 2-2 iC
3-1 1 0
3-2 lo
4-1 1 h
___L2_______ _ _ i _______ _JJ2____
__________
1 1 1 _____
4-2 i n S-l I O 5-2 i b
_ .--------O
D
r\
jlO________ Tin: t<'V$ r?afl--
M v ^rteigit tohia .
Scuro; to r e g -
Hitch (2zlJASQtel.rmI.onwit. HWMASW S , lh*r------- SJimriii. Actlumioft (nTQ*^ 1 ^ ,0 n. o .a
h a * ? poor .0 Wat:
J H . ---------->S 0lh^ - ------------------
a* ~ -
O ^ r a u a : *"<(2 * ^ D ,00DrfD
a - p '"'D *0," D 0th,r-
IrabWro. o^rVTi po' r*ji**r:
J ri ri
P m ia p -w t 16L:8D 3 Ol>r.
ao... | 2 - l t m _ T.WrA--0 3
SETUP: I
ImAronriiAdfed.X fc lfi- SrtUpBf_CcL2
INSIHUMENIATIONUSED: SiamTUoromatarNxrtc.A i -
(Form AMBA)
fw O
Dv 1
dt ?
c rr? r' =>*? rw.G
1 jdXry>. \-- 2 a A
2 . 3l 1 C <
3 a a .^ 2.0 <
4 lb o
2f
5 Qo.<~
*0*1-
c tf.a v ' S . O cl g-<^_
1 * :>w * . >1- s./o
2 . 2 N . (4
-
3 A./3- ff. ite - _ X i i -
4 tf. l
5 . 0 ff-2*2- r_ M _
C _ 2 ^ L . ^ l _
1 x .f
7- 9 _ 2 i --
2 If
7. f 7 9
3 4 # ./\ 5 *A C 3 C
_L _ 7. Z \
7. 7
_7J!___
7.9
7 - .-y 5
71 _
1 as 2 X>K
X1 21
iz i JX
3 a n 10 a _2L t_
4 5 s . Z
OA__
s
ititi # r. Intuit:
D K 2.0 I2
<^3-
I T i3
NOTES.
Y3SCI33Ua.ar1H80IS7S2)
Other
1S(*^n*TCWr'ctOT"rt*3M)
</
TEST I D.:
^ iCV
D-281
US00001462
TRIMMED SPEARMAN-KARBER METHOD. VERSION 1.5 -
DATE:
1 2 /2 /9 7
TOXICANT : B3
S P E C IE S : M BAHIA
RAW D A T A : C o n c e n t r a t i o n
.0 0 6 .2 5 1 2 .5 0 2 5 .0 0 5 0 .0 0 1 0 0 .0 0
TEST NUMBER: JR A 9 7 1 6 6
D U R A T IO N :
N um ber E xposed
20 20 20 20 20 20
M o rta liti
0 0 0 0 2 20
SPEARM AN-KARBER T R IM :
.0 0 %
SPEA R M A N -K A RB ER E S T IM A T E S :
LC50:
95% LOWER CON FID EN CE:
95% U PPER CONFIDENCE:
t
6 5 .9 8 6 0 .1 2 72.41
48 H
D -282
US00001463
COA.STAI. HIOANALYSTS. INC. NPDES STATIC ACLJTE/SALTWATtR
<Kurm ACV4)
V
TEST 1.D. :_ _ 2 j k A S 2 - d D-283
US00001464
COASTAL BIOANALYSTS INC. EFFLUENT/DQ.UT10N WATER DATA <F Sample Date
Salinity (g/kg) 1 Q
pH________I % f?- I
P iss. Oxygen (mg/1) j / , *7 J
Tot. Chlorine (mg/1) 1 P L 1
NH3-N (mg/1) ! 2 lo
Hard (mg/1 CaCQ3) Alkln (mg/1 CaCQ3) 1V 3
C olor/Appearance1 [ ^
Initials
!> ^
1 ................ ' , . -T-
s. -Sohds (S-SI.ght M-Moaerate V-Vcry Mucn)
t r .r i e a r O-Opaque YF ellow B-Brown Bl-Black G-Oreen 1__________ _ _______________- ______________--
EFFLUENT D rf _
X JJ*?1/
_TEST ED ^\(L 1 :D-284
US00001465
Toxicity Test Sample Chain of Custody (Please complete all information)
Facility,
Address__JOor-fe?\yc v/A
%
County,
Pipc/Outfall/Location
NPDES#_
Instrcam Waste Cone
Sample'coilected by (print&sign) i
H
Affiliation fiPH- W . Eya. \ _ g U
Type of sample
(Grab):
Date l Z / P i / ^ 7 Time & : 6 ~ j t y t \
______ (Composite): From Date_________ Time____________
To Date
Time
Subsamples comprising composite:
Number___________ Frequency of collection_________ Volume,
'Temperature of sample in sample collection device 0 * ( j /
Final temperature of effluent at sample collection point.
Is sample collection device chilled?
Is sajnplc packed on ice for shipment?
`It is required that all samples remain at 0 - 4C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency (Do not freeze!)
is 'the sample chlorinated?
dcchlorinated? ho Iif so., hooww??_V o .
Permit with interim chlorine limit?. -- If yes - limit (mg/L)_
Field pH % \ Q
Field Total Residual Chlorine
Comments/Sample dcscription_
Type of test(s) to be performed
ftW SoVp . C r
(Specify organlisms)
Vv ?a1r r e ^ a - f u s
J
Method of shipment.
r n n t & Sign Names
i. Relinquished bv D r.
Date 11 - / 0 2 - / *C7
Received by__ . Date
Relinquished bv ^ ^ 7
Date /
Received b y _ s 2
<Jl
Date
,,T~
Time
0Q ~^\
Time - - - - Time / O ' f O
FOR REED LAB USE ONLY
JRArf
*3 G? *~7 C~j> Arrival Temperature
Color______ ___________________ Odor .
pH_________ DO (mg/L)_________ Conductivity (pmhos/cm)
Salinity (ppt).
TRC (mg/L)______ Method
On ice?
D-285
* 5/16/9
US00001466
Print & Sign Names
Relinquished by_________ __________ Date_____________ Time
Received bv
* Date U
Time
Relinquished by^7i f >--
1 Date
Time_______
Received bv Pcv^~~^M )Ooo<^
Date 1 3 - ^ - 9 ^ Time >3-36
D-286
US00001467
Coastal Bioanalysts Inc.
O D U /O cean a Acute Effluent Toxicity Tests
M ysid o p sis baha & C yp rin o d o n va rieg a tu s
Sample I.D. B2 Contract No. N00187-93-D-9748
Delivery Order No. 0235 J.O. No. 1912290, Item No. 0002BJ, 0002BH
* PWCNo. 1393 12/1/97 1725 Sample No. JRA97-13675
Submitted to: Ms. Merrill Anderson-Ashcraft
Navy Public Works Center Code 930, Building Z-140
Norfolk, VA 23511
By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062
(804) 642-0168
D -2 8 7
US00001468
O11D1I.,9'.(7JCSEaAmNpAle 9S7a-m13p6le75I.D.TBe2st ID: J. RA97I63
Coastal Bioanalysts Inc.
I. TEST RESULTS
% Effluent Concentra tion
0
6.3
12.5
25.0
50.0
too
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acute Test Results
Mean (%) Organism Survival
M . bahia
C Variegatus
24-h 100 100 100 100 100
70
48-h 100 100 100 100 80 5
2AJi 100 100 100 100 100 100
48-h 100 100 100 100 100 100
63.9% 55.2-74.0 Trimmed Spearman-Karber
1-56
> 100% N/A N/A < 1.00
Acute Test Information:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
M. bakia Test
48-h Static
Survival 12/2/97 1710
E
12/4/97 1655
Chesapeake Cultures, Inc.
12/2/97
20 g/kg
19.0-20.7C
No
N/A
C variegatus Test
48-h Static Survival 12/2/97 1715 12/4/97 1650 Aquatic Bio Systems, Inc.
11/30/97 20 g/kg 19.0-20.7C
No N/A
Aeration necessary when D.O. concentration <4.0 mg/1.
D-288
US00001469
Oi nDI'9O7CSF..LAmNpAk' 9S7a-m13p6le75IDTBe2st ID: JR.A97I65
Coastal Bioanalysts Inc.
Sample Collection Pate/Time
12/1/97 1725
Sample Use Dates/Times
12/2/97
.
1710/17153
Aeration2 Rate/Time
n/a
Pretreatment/ Adjustment
HWM Salts
2Aeration often necessary to reduce sample oxygen concentration to 100% saturation after warming. 3.V/. bah ia'C . variegatus test time.
Table 2. Effluent and Dilution Water 4 Chemical & Physical Characteristics (Mean Value (Standard Deviation))
Param1e-ter Temperature
(C)
Salinity (g/kg)
Conductivity (uMHOS/cm)
pH
Dissolved Oxygen (mg/1)
Total Hardness (mg/1 as CaC03)
Alkalinity . (mg/1 as CaC03)
Total Chlorine (mg/1)
n h 3-n (mg/1)
Initial Effluent
2.6 (--)
2 (-)
2610 (-)
8.22 (-)
12.7
(_)
: 209 (-)
392 (-)
<D.L. (-)
3.0 (~ )
Adjusted Effluent 20.5
(-- )
- 20 (...)
N/A
8.02 (-) 7.9 (--) N/A
,,
N/A
N.D.
N.D.
Dilution W ater 20.1 (-) 20 (-) N/A
8.25 (-) 7.9 (-) N/A
N/A
N.D.
N.D.
A ffluent parameters measured upon receipt of sample in lab and after adjustment on day of use. Dilution water (Wimex hw-Marincmix in ASTM Type I deionized water) values measured after preparauon of diluent on day of use.
D -2 8 9
US00001470
O12D/1I.9'O7CSEaAmNpAle9S7a-m13p6le75l.D.T8e2si ID: JRA97165
Coastal Bioanalysts Inc.
II. WATER QUALITY
Parameter
Temperature (C)
DO. (mg/1)
pH
Salini tv (g/kg)
Caotcal
20.4 < (0.3)
7.9 (0.1)
8.14 (0.09)
21 (1)
Table 3 Water Quality Summary
M . baha Test
Mean Value (Standard Deviation)
6J%
12.5%
25%
50%
20.4 (0.3)
20.4 (0.3)
20.5 . (0.2)
20.5 (0.1)
7.8 (0.1)
8.16 (0.13)
21 0)
7.8 (0.2)
8.16 (0.10)
20 (0)
7.8 (0.2)
8.16 (0.04)
20 (0)
7.8 (0.2)
8.15 (0.04)
20 (0)
100%
20.5 (0.1)
7.7 (0.3)
8.14 (0.10)
20 (0)
Parameter
Temperature (C)
D.O. (mg/1)
pH
Salinity (g/kg)
Conned
20.6 (0.5)
7.9 (0)
8.15 (0.09)
21 (1)
C. varUgatus Test
Mean Value (Standard Deviation)
63%
20.6 (0.4)
12.2%
20.5 (0.3)
25%
20.6 (0.3)
2%
20.6 (0.3)
7.8 (0.1)
8.17 (0.12)
7.8 (0.1)
8.17 (0.09)
*7.8 (0.1)
8.15 (0.04)
7.8 (0.2)
8.14 (0.04)
21 20 20 20 0 ) (0) (0) (0)
100%
20.6 (0.2)
7.7 (0.3)
8.11 (0.08)
20 (0)
-
D-290
US00001471
ODI' <XXANA Sample ID. B2
12 -1 9 " Sample 97-13675 Test ID: JRA97I65
m . OUTFALL DATA SUMMARY
Coastal Bioanalysts Inc.
Test Date
9/30/97 11/3/97 11/25/97 12/2/97*
Table 4. Cumulative Data Summary - Acute & Chronic Tests (Tests conducted on this outfall by CBI)
Invertebrate
Acute Tests
Chronic Tests
I.C50 U L
M.QEC U L
V erteb rate
Acute Tests
Chronic Tests
LC50 U L
NQEC U L
66.0% 50.0% 42.1% 63.9%
1.52 2.00 2.38 1.56
N/A N/A N/A N/A N/A N/A N/A N/A
>100% >100% 72.6% 72.6%
<1.00 <1.00
1.38 1.38
N/A N/A N/A N/A N/A N/A N/A N/A
Results submitted with this repon
IV. QUALITY ASSURANCE
M. bahia - Acute
Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
C variegatus - Acute
Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
Chesapeake Cultures, Inc. (same batch as test) 12/2/97-12/4/97 KC1 (Sigma "Ultra" Lot#76H10391) 343, 490, 700, 1000 mg/1 100% 606.9 mg/1 (578.6-636.7) 608.2 mg/1 (489.3-727.0)
Aquatic Bio Systems, Inc. (same batch as test) 12/2/97-12/4/97 KC1 (Sigma "Ultra" Lot#76H10391) 432, 720, 1200, 2000 mg/1 100%
1169.7 mg/1 (1 0 4 4 :1 -1 3 1 0 .5 )
1074.9 mg/1 (890.8-1259.0)
D-291
US00001472
CUMULATIVE DATA SUMMARY NPDES Permit #: N/A Oceana - ODU B-2
Date o f J." Invertebrate*:^. Test
2/12/97 >100 2/28/97 Invalid 3/12/97 >100 3/20/97 >100 3/26/97 >100 9/30/97 66 11/3/97 50.0 11/18/97 93.9 11/25/97 42.1 11/25/97 63.9
Yertbrate-^lc-.'-'-.X; Invertebrate
>100
n e c % ^-:
>100
>1Q0
>100
>100
>100
>100
>100
72.6
72.6
" Vertebrate ' N O E c % ~ Y ;|
Supplement
D-292
US00001473
Coastal Bioanalysts Inc.
ODU/Oceana Acute Effluent Toxicity Tests
M y sid o p sis b a h ia & C yprinodon va rieg a tu s
Sample I.D. B 1 Contract No. N00187-93 -D-9748
Delivery Order No. 0235 J O. No". 1912290, Item No. 0002BJ, 0002BH
PWCNo. 1392 12/1/97 1925 Sample No. JRA97-13674
Submitted to: Ms. Merrill Anderson-Ashcraft
Navy Public Works Center Code 930, Building Z-140
Norfolk, VA 23511
By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062
(804) 642-0168
D-293
US00001474
O D f - O C E A N A Sam p le I D . B I
12.1-97 Sample 97-1367-4 Test ID: JRA97164
Coastal Bioanalysts Inc.
I. TEST RESULTS
% Effluent Concentration
0
6.3
12.5
25.0
50.0
100
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acute Test Results
Mean (%) Organism Survival
M . bafiia
C. variegatus
24Ti 4 H
2 is h
4M
100 100
100 100
100 100
95 95
100 100
100 100
100 100
100 100
100 100
95 95
95 70
100 100
>100% N/A N/A <1.00
>100% N/A N/A <1.00
Acute Test Information:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
M. bahia Test
48-h Static
Survival
12/2/97 1650
12/4/97 1705
Chesapeake Cultures, Inc.
12/2/97
20 g/kg
19.0-20.7C
No
N/A
'Aeration necessary when D.O. concentration <4.0 mg/1.
C variegatus Test
48-h Static Survival 12/2/97 1650 12/4/97 1710 Aquatic Bio Systems 11/30/97 20 g/kg 19.0-20.7C No N/A
D-294
US00001475
'OCE.XNA SampleI.D. Bl .9? Sample97-13674 Test
ID:
JR.A37I64
(23)1Coastal Bioanalysts Inc.
Sample Collection Date/Time
12/1/97 1925
Sample Use Dates/Times
12/2/97 1650/16503
Aeration2 Rate/Time
n/a
Pretreatment/ Adjustment
HWM Salts
2Aeration often necessary to reduce sample oxygen concentration to 100% saturation after warming. 3A/. bahia/C. variegatus test time.
Table 2. Effluent and Dilution Water 4 Chemical & Physical Characteristics (Mean Value (Standard Deviation))
Parameter
Initial Effluent
Adjusted Effluent
Dilution W ater
Temperature
3.4
20.8
20.4
(C) : ( - ) (--) ( - )
f Salinitv 2 20 20
(g/kg)
(...) ( - )
(--)
Conductivity (uMHOS/cm)
2580 < -)
N/A
N/A
PH
Dissolved Oxygen (mg/1)
8.46 (-)
12.0 (-)
8.05 (-)
7.9 (-)
8.10 (-)
7.9 < -)
Total Hardness
" 193
N/A
N/A
(mg/1 as CaC03)
(-)
Alkalinity (mg/1 as CaC03)
Total Chlorine (mg/1)
n h 3-n (mg/1)
' 367 (-)
<D.L. (-)
9.6 . (~)
~N/A N.D. N.D.
N/A N.D. N.D.
4Efflucm parameters measured upon receipt of sample in lab and after adjustment on day of use. Dilution water (Wimex hw-Mnrinemix in ASTM Type I deionized water) values measured after preparation of diluent on day o f use.
D-295
US00001476
CUMULATIVE DATA SUMMARY NPDES Permit #: N/A Oceana - ODU B-l
Date.of 5 Invertebrate^ Test A -
2/12/97 >100 2/28/97 Invalid 3/12/97 >100 3/20/97 >100 3/26/97 >100 9/30/97 72 11/3/97 50.5 11/18/97 >100 11/25/97 40.2 11/25/97 >100
Vertebrate
>100 >100 >100 >100 >100 >100 >100 >100 >100 >100
Invertebrate, r .
Vertebrate ' s" T NOEC % ` _ .~ i~
Supplement
D-296
US00001477
CASTvU. DI AN'.AJ-VSTS, INC. NPDE5 STATIC ACUTE/Sa LTW a TE
(Forni A.McH4;
1 EHI--"I
I.D.
0*y 1 1 La
0*2 9 La
Krwi \
Survn!
Control
6.25 12.5 25 SO 100
C-1 n C-2 i o
____L S _______ 10
1-1 1 O
(o
1*2
2-1
2-2
3-1
3-2
4-1
4-2 CTT"
5-1
lo
Io
(O
(0 )0 (0
(6 (6
_ u > ______ 10 ib
10 1b 1b
(0
1
5-2 l Oburv*r >- p 7 j -
...
n
____
Tigg--j _i.Ccfc>. .. l n o 5
10O
\O D \0 o \ O
(0 o
10
-V-f!
.,1
... -4
TEST I .D .:_ _ 1 l5-LL^5.
D -297
US00001478
C O A Sr.U . UIO,\NA3-YSTS. INC. NPDES STATIC ACUTH/Sa LTWATHR
(Komi ACV-*)
\ Eltli--n
Contiti
6.75 I2.S 75 SO
100
1.0. 9D*Inrs1
C- IO C-2 / u M fU
Dr7
La IC 10
\0
1-2 _ _ 5 _____
2-1 1 0
1o
2-2 1 0
3-1 i O
3-2 f0
4-1 1 0 4-7 y S-l I0 5-2 1
. l a _____________
_J_a___
_ _ L 2 ______________ ! O--------Y
t o ______ /o
ObMj w . Tut:
<n
/fP-C-
P&_ I n lt>
ht* \ \o o
io t> 1DO
\ O0
c- . .. fu n rin a d o n van#03tuS
w c r*i _____________ __________
A rchnaconnilA r HWU A 3 * {
0u" r-.
/ ^3 ^
IO
SNm tr. " 2 - 0
Z-O-3------
O lW .
SOF - a . y in n , i l : Hn M
3 0 0 ,W D
500
/d Oth: 1 0 0 0 H O tta r
-
T . k TM 2 r t D
750 n
5 0 0 J 2 O tta r_________
In tu no o fm n n (n n d *n :
1 Q ------ _ m .m *a trn :jQ -------------
n m n n in t i6 ij o g p s ita r._______ m m - l ? y . .
cfT ii^ n --
A?"{"? / 2 -
T iw A M
(/V 5
.
T TM . A n n a li A d M 1 ^ 5 6
SnU oS. 6 ^ / F'K
IfCilHUMtSUIlUNUiO:
4 -Staro ThwnnraMf JAarbor
TS D.O. M .w f I ISNEisBm0144?7'SI
gCP fH taur (09500563) S tfio rn . HI SOP B W n . (4020063) T3 SCT 33 kfcm (H8016792)
gr s O.a Ita u r 2 (SN91A026329) C nm n,240|*JtW (S >626i)
Pn*rt2fcta(52360
3 S p n t* ATC W m u < IA368)
O ltat
DnO On1____ On?
C WY (7 Y ,30-5 r 1 ^4>.V -7,1 b O
E
u
2 P f- 3o V _ Ad-k
3 - n.o
Pi
4 -Dp.ff 10-6 ,90-1
5 7u. 7 y i L
c fi. /6 tf.o?
F. d f T o2r t o ? . .
P
2 ff.df
?.|0
3 .* 'G
4 S.oV
i?.o?
S S'-S'
c 2.? 1 n-.i
0.0. ? 7-1
fi o ._ 7 ? 7 ? 7 7
7 <V n .o
W) 3 7 . 9 4 7.1
1 9 _ 7-^r-- l i 7.7
5 79
7C>-
sc
A L
1 Ht 2 Jo
H 3 Jn
Al P( 91 ._ 2 | 20 0-> : 20 5-0
Ib^j) 4 <3b 5 O)6
'Z.G 20
<9^
Raficil(Jf )
2- 1
Inltti*: _Gfi___ s o _.ffa -
NOTES:
TF.ST1.D.: S(lf\*3JMtL
D-298
US00001479
1NITLAL (ARRIVAL) EFFLU EN T CHARACTERISTICS:
Sample Date \ i z n m l-
I
j_
i
i
i
Temp (C) \ t>m
!
1
i
ii
Conduct. (uMHOS) ISfSL 1 1 i i i i
Salinity (g/kg) 1 1 1
ii i
pH
IS.yo, li
1
i
ii
i
Diss. Oxygen (mg/1) 1 1 l i
Tot. Chlorine (mg/1) 1 L V<- |i
li ii
NH3-N (mg/1) 1 i> 1 1 i
ii
Hard (mg/1 CaC03) I m
1
1
i
ii
i
Alkln (mg/1 CaC03) I 3 u a 1 1 i i
i
Color/Appearance1 r /
r
i
i
ii
i
1 1Initials
c-e> r
i i i ii
1 C-Clear O-Opaque Y-YeUow B-Brown Bl-Biack G-Green T-Turbid SI -Solids (S-Slight M-Moderatc V-Very Much)
FFFLUENT ADJUSTMENTTOST-ADJUSTMENT MEASUREMENTS:
ACUTE
Date
|
Temp. (C) |
Salinity (g/kg) |
D O. (mg/1)
Aeration Rate ,- |
Aeration Time (min) |
A d ju st. D.O. (mg/1) |
pH | Tot Chlorine (mg/1) |
Initials
I
| 1 1
I | | | I
1^ 11 11
I 11 11 II |1 11
DILUTION WATER CHARACTERISTICS:
DMW:
Date -
1
1
1
1
Temp. (C) | | | 1
Conduct. (uMHOS) | | 1 1
Salinity (g/kg) |
111
pH t i l l
Diss. Oxygen (mg/1) |
|.
|
Hard (mg/1 CaC03) | I I 1
Alkln (mg/1 CaC03) |
1
1
Initials
|
111
l 1 1
1 1 1 1 1 1
H W M :v/
1 f I
1 1 1 1
1 1 Il/S/Drt?
1 1 II5 &,?
1 1 II D a 1 1 II 7-.1
1 1 II -- 1 1 II " , 1 1 II ' 1 ii s \ o r 1 1 Ha/ p ___ 1 1 II
ACUTE
1 11 11 1 11 11 11
-1
11
ii a ^
\UrQ
II t n
II9-16
li m ____
II /M/211 0 0
O
E F F L U E N T ID: P) I_______________________ TEST ID:j T
fa *"(--
- D-299
US00001480
Toxicity Test Sample Chain of Custody ;(Please complete all information)
Facility, Q \c L D o m i n i o n Address^" V s o r-fo w . y A = P 3 5 < g q
%
County
^ ______'______;_____
Pipe/Outfall/Location
NPDES# - ____________ ' '
Instrcam Waste Cone
____________ ___
Sample'collected by (print&sign).
Affiliation. C Q tl &r*j.
. laVy
Type of sample
(Grab): : 'Date. \Z./Q\
Time 7 ;35*j* v \
. ______ (Composite): From Date__________ Time_____________
i To Date_____________ Time_____________
Subsamples comprising composite: % .
. Number______ :
Frequency of collection.
Volume.
'Temperature of sample in sample collection device 0 *0/ Final'Temperature of effluent at sample collection point. J t . Z ' a
'Is sample collecrion device chilled?^ a _ Is sample packed on ice for shipment? M p3
'It is required that all samples remain at 0 - 4C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency (Do not freeze!)
Is the sample chlorinated?.
d e c h l o r i n a t e d ? 1^0 If S O , h o w ?
______________________
Permit with interim chlorine limit?.
If yes - limit (mg/L)_
Field pH % -?L
Field Total Residual Chlorine.
Comments/Samplc description. 3 L
Type of tcst(s) to be performed
^Vy
(Specify organlisms)
e ^ q - h t g ________________________
Method of shipment TVfrvonn, Ym D t i
V n n t & Sii;gn Names
^ -0 0
1 . Relinquished by IV. M
l Date'. ix / o i. / 17 T im e -f O ' G O ' t *
Received by /?_
I 00au-- Date A p /,^ /f fZ . Time 0*7 f < T tjf /
Time / &
Time.
JRA# \ 1 - l3 Q p ~ fLf
FOR REED LAB USE ONLY Arrival Temperature__________
Color_____________________ ;_____ Odor____________ _
pH__________ DO (mg/L)
'Conductivity (p.mhos/cm)_
Salinity (ppt)_______ TRC (mg/L)______ Method__________
On ice?_ Solids.
fa C ** oW
D-300
US00001481
Relinquished b; Received'by.
Print & Sign Nam es
Date _____ D ate (SI'ejk-^7
D a te ( ^ -~ g ^ ~ ~ ^ ^ 7 H a fe /~ 3 -- - 3
Time_____ Time_____ _ Time_^._____
T im e p - q / l
D-301
US00001482
D-302
US00001483
Coastal Bioanaiysts Inc.
, ODU/Oceana Acute Effluent Toxicity Tests
M ysid o p sis bahia & C yprinodon v a rieg a tu s
Sample I.D. A3 Contract No. N00187-93-D-9748
Delivery Order No. 0235 J.O! No. 1912290, Item No. 0002BJ, 0002BH
PWCNo. 1391 12/1/97 1725 Sample No. JRA97-13673
Submitted to: Ms. Merrill Anderson-Ashcraft
Navy Public Works Center Code 930, Building Z-140
Norfolk, VA 23511
R
By: Coastal Bioanaiysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062
(804) 642-0168
D-303
US00001484
O12D1t'.O97CES.aAmNpAle9S7a-m13p6le73I D.T.Aes3i ID: JR.A97163
Coastal Bioanalysts Inc.
I. TEST RESULTS
% Effluent Concentration
0
6.3
12.5
25.0
50.0
100
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acute Test Results
Mean (%) Organism Survival
M. baha
C. variegatus
24-h
48-h
24rh 4 3 i
100 100
100 100
100 100
100 100
100 100
100 100
100 100
100 100
100 100
i 100 100
95 95
100 100
>100% N/A N/A <1.00
>100% N/A N/A <1.00
Acute Test Information:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
.
M. bahia Test
48-h Static
.Survival
12/2/97 1635.
12/4/97 1535
'
Chesapeake Cultures, Inc.
12/2/97
20 g/kg
19.0-20.7C
No
N/A
C variegatus Test
48-h Static Survival 12/2/97 1620 12/4/97 1520 Aquatic Bio Systems, Inc. 11/30/97 20 g/kg 19.0-20.7C No N/A
'Aeration necessary when D.O. concentration <4.0 mg/1.
D-304
US00001485
ODCOCKAN.A Sampli ID. .AJ
12.1 97 Sample 97-13673 Test ID: JR.A97163
Coastal Bioanalysts Inc.
Sample Collection
12/1/97 1725
Sample Use Dates/Times
12/2/97 1635/16203
Aeration2 Rate/Time
n/a
Pretreatment/ Adjustment
HWM Salts
^Aeration often necessary to reduce sample oxygen concentration to 100% saturation after wanning. 3.W. bahia/'C. variegatus test time.
Table 2. Effluent and Dilution Water 4 Chemical & Physical Characteristics (Mean Value (Standard Deviation))
Parameter
Temperature (C)
Salinity (g/kg)
Conductivity (uMHOS/cm)
pH
Dissolved Oxygen (mg/1)
Total Hardness
(mg/1 as CaC03)
Alkalinity (mg/1 as CaC03)
Total Chlorine (mg/1)
n h 3-n (mg/1)
Initial Effluent
3.5 (-)
1
(...)
980 (-)
8.01 (-)
12.1 (-)
161 (-)
257 (-)
<D.L. (->
2.2 (~ )
Adjusted Effluent 20.9
(...)
20
(...)
N/A
8.07 (-) 7.9 (-) N/A
N/A
N.D.
N.D.
Dilution W ater 20.3 (-) 20
(...)
. N/A
8.14 (-) 7.9 (--) N/A
N/A
N.D.
N.D.
4Effluent parameters measured upon receipt of sample in lab and after adjustment on day of use. Dilution water (Wimex hw-Marinemix in ASTM Type I deionized water) values measured after preparation o f diluent on day of use.
D-305
US00001486
O D I'.tX
12/1v i
'ESiAmNpAle
9S7i-m13p6le73t.D.TAe3st
ID:
JRA97163
Coastal Bioanalysts Inc.
II. W ATER QUALITY
Parameter Temperature
(C) DO. (mg/1) PH
Salinity (g/kg)
Parameter
Temperature (C) DO. (mg/1)
PH
Salinity (g/kg)
Control
20.4 (0.2)
7.9 (0.1)
8.07 (0.07)
21 (1)
Table 3. Water Quality Summary
M . bahia Test
Mean Value (Standard Deviation)
6 3%
20.5 (0.1)
7.9 (0.2)
8.08 (0.08)
12.7%
20.5 (0.2)
7.8 (0.1)
8.10 (0.07)
25%
20.6 (0.2)
7.8 (0.2)
8.11 (0.06)
50%
20.6 (0.1)
7.7 (0.2)
8.17 (0.08)
20 20 20 20 (0) (0) (0) (0)
Control
20.3 (0.1)
7.9 (0.1)
8.05 (0.08)
20 (0)
C. variegatus Test
Mean Value (Standard Deviation)
6.3%
20.4 (0.2)
7.9 (0.1)
8.05 (0.09)
20 (0)
12.5%
20.4 (0.3)
7.9 (0.1)
8.05 . (0.07)
' 20 (0)
25%
20.4 (0.3)
7.8 (0.1)
8.07 (0.05)
20 ()
50%
20.5 (0.3)
7.7 (0.2)
8.15 (0.06)
20 (0)
100% 20.7 (0.2) 7.7 (0.2) 8.22 (0.13) 20 (0)
100% 20.6 (O.j ) 7.8 (0.2) 8.22 (0.13) 20 (0)
D-306
US00001487
O12D.MI-9O7CSEaAmNpAle 9S7a-m13p6le73l.tTAes3t ID: JRA97163
ID. OUTFALL DATA SUMMARY
Coastal Bioanalysts Inc.
Test Date
9/30/97 1 1/3/97 11/25/97 12/2/97*
>* *
Table 4. Cumulative Data Summaiy - Acute & Chronic Tests (Tests conducted on this outfall by CBI)
Invertebrate
Acute Tests
Chronic Tests
LC50 T.U.
NOEC T.U.
>100% >100% >100% >100%
<1.00 <1.00 <1.00 <1.00
N/A N/A N/A N/A N/A N/A N/A N/A
V erteb rate
Acute Tests
Chronic Tests
LC50 T.U.
NOEC T.U.
>100% >100% >100% >100%
<1.00 <1.00 -, <1.00 <1.00
< } j / A \ N/AN/A-1- N/A N/A' N/A N/A N/A
`Results submined with this report --
- -- --------
IV. QUALITY ASSURANCE
M. bahia - Acute
Reference Test Dates: Reference Toxicant:
. .
.i,"
, r,n - rv
Test Concentrations: .
Control Survival:
LC50 Reference test (95% C.L.):
Control Chart LC50 (95% C.L.):
t
C. variegatus - Acute
Reference Test Dates:
Reference Toxicant:
Test Concentrations:
.
Control Survival:
LC50 Reference test (95% U.L.):
Control Chart LC50 (95% C.L.):
\ (V
Chesapeake Cultures, Ihc. (same batch as test)
12/2/97-12/4/97
.. ; '
KC1 (Sigma "Ultra" Lot#76H10391)
-
343, 490, 700, 1000 mg/1
,
100%
,
606.9 mg/1 (578.6-636.7)
608.2 mg/1 (489.3-727.0)
_
Aquatic Bio Systems, Inc. (same batch as test)
12/2/97-12/4/97
KC1 (Sigma "Ultra" Lot#76H 10391)
432, 720, 1200, 2000 mg/l
.
100%
-.
1169.7 mg/1 (1044.1-1310.5)
-
1074.9 mg/1 (890.8-1259.0)
D-307
US00001488
CUMULATIVE DATA SUMMARY NPDES Permit #: N/A Oceana - ODU A-3
Date o f ^T nV ettebrif^^r- -S-.7', yei^bratei^J5/S-:l?^ Invertebrate TestO^;;; L G 5 0 % 3 f g p ^ ^ '
2/12/97 >100
>100
2/28/97 Invalid
>100
3/12/97 >100
>100
3/20/97 >100
>100
3/26/97 >100
>100
9/30/97 >100
>100
11/3/97 >100
>100
11/18/971>100
>100
11/25/97 >100
>100
12/2/97 >100
>100
' - YertebrdteTT..:
Supplement
D-308
US00001489
COASTALBIOANALYSTS. INC. NPDES STATICACUTE/SALTWaTE
(Fot A M b ^ )
*EtfW 1.0. 0fl/yYl*
Contrai
C-I 10 C-2 |0
M io
6.25 1-2 rO
21 |t
12.5 22 lo
3-1 \ 0
32 \G
4-1 i 0
50 4-2 \ b
51 10
100 5-2 t ObMrvf. 7 itotT
Titrac<1 s-V
D#Ury*2a to (o \o
_I ____ 19
lo
10 19 (0 10
,12---- -
-
_ _ -----
fSirumrviiv1al 1oo \ CO
lo o 1.00 \o o
c,-- My s i d op s i bahia______________
Scum:
T3TC _
Hwch ta lm .u o i.w .1 HWMASW 0
--------------:----------------- -- < * :_ ------------------- ,S n t r _ 2 ^ _
Acdvm uxi Nmp- Irang*!1_______ ^ ^ -- --------U-- ^ 0 . 0
Fm^(^priortott: An* j ^ Ji ---------- --
FMdngdnnglBC t e il" ! D
A n *rm J _ }c -----/ d 0 d w ;------
C h " *" .: * P
500
W D 0U -:_
T -tv o to r.: 2 0 0 ^ p
S O ^ Q
SOOni O t>-r_
Initialno.Of^ftwrpatrafi*ai*_ /O -
ftatopanod 161:30 ^ 3 ' O tff.
SET UP: ... ' \ t l Z M n _ Tmm to - AM l \ i ! 5 --------------
Jam A m rjaA M
I K x V ? _____ Up _ V f i / f X
INSTRUMENTATION USED:
Siam Thamwnalar Nuifear,M
-
YS D.O .hUur f I (SNEBOl7S )
|c P ft -- > M .U fIP S 5 a B I3 )
I SronyBI6GP M m 140020083)
YS X T 33 M i" (H80167K2J
Othaf
,,,, Q jY 3 0.0. U .-I2 {S W 1 *0 2 3 2 9 | n to , 240 ( * U - tS * * l
O SPPaH (*C U " SS236C) ^ 5#*un ATC flp ftic o x ie -- IA366)
DrO____ OnJ____ 0*v2
C Qb 3>
t? ptf.y
T 1 3.<r ?C G S>-o.y
E U
2 3o.? 3 a.-?
7o.s7 7
<?0 3 Po-3
4 3c'- 7 -Po-5
5 3 0 .5 7(7-6 ^ - s
C /T 7
, ft.w
75*)
(
2 ?./?>
/ V 9 07
3 ff./f < r /7 %oS
4 ?.o,P
<-
5 e T-.f
YT-aT- T-30 ft-6 - V 8
1 TU?
ft. ft _2_2... ______
0.0. 2 > . f
7- . 7-7 ._
3 ?-.f
-- --p e?
4 9-. 7.?
7-5
5 2-7
_ 7.5 .
S A
C 1_7Oa' f -- 2 ( <*
1 3a
zo r
D-[ P
l2
2.0 P-C>
N 3 Pa 20
0*0
IM 4 Q 5 3n
RafieU9 /
?Q -- P o 2-O <P> 7- (
Intua: -- 3?-- - Pe
NOTES.
TEST m -
3
D-309
US00001490
co-vrr.vj. uioanalysts. inc. npdes static acitIvSaltwatlk
(t-'TMA<-'v-u)
lE lIk *
CucUtJ 6.25
C-l c? 1-1 12
21 12.5 2 2
3-1 25 3-2
4-1 SO 4-2
SI 100 5-2
0t*arvr
Tm:
Dayl 1 Lw* |0
(0
10
YU
70
/t>
l0 lb ib lb I0 lb P& H ,3 o
D a r'2 Lm
. 7
(O
' fir il S nvd
( oc>
lo l6
\ 06
(O
t o ______ l0
(o
I O
)Oo
r I P _______ U
(b o
(o
10 L b o
ft
.
So m .
X A O S '
Hatch O-taTim s:
1M W 5 1 .
AccUmation watsT HWU ASW ^3
OUiar
Acl'IuiwI kwi T a ty i {rw ^ p f
Z'?-
- 7 -/> "7 - ..
f w d 'S teir im i N u tad {Q Ananaa
14 Otte:
300-
5 0 0 - 1 0 0 0 - 0 Odiar_________-
T u rvckrm: 2 0 0 -
2S0-
(Aldino.oQvwTiparwakm: fQ
ft*upanotf l&l.10
Qth*:
0 - j a O tte:
M
- ^ w^ail,:jQ
I^ IA
SET UP. D io
f 2 -/
T in* Annata Atatad^ I b - O
S -U p d r f r f V P T ?
IfC 'im jM tN U IlO N U S iD :
r ASlamThannomaur Mar-- t YS 0.0. Maw f I (5MEIOM75)
CPD ^m m |fl Malar (09500663) Sariem* R160PB a i* (40020093) 13 YS SH 33 Malar (H8016792)
YS 0.0. Malar 1 2 ISWU02S32J Corning240 04 Maur (96261)
SPP w li( -a la r (S52360) P *m o a *TC FWiKttamtar (U M )
DayO
Day 1
Oar 2
C 7c- 5
3D T
2 c; 4
i 3 a h 7 Do 1
u
3 <3 0 .4
DO-Z
4 S a .y L
D o l'
5 3 a /7
D '-o
C 8 ,/y
% o'*
1 6 ,/y
Dr O' r
2 ./3
f t P.D
(*
3 9y.ll
ft. f a
4 ff.of f t . / ?
S S.D c
1 I'T '.l
Z. . 1-
7f a l?
0.0. 2
7.9
(yi 3
7 ft _
~ v [ 7 <5
5 P -.f
7 .5
s C On
<ccr
A
L
1 Dp<tU\ -------) C.O r
2 r?
20
N3
2o
4
toAl
S
Aapfacau I
In iia ta :
4 (i 46
f
< r _
Z. 0 20
Z
So
9 0 y
>o.H Oo 3 POD
.P . 5 c4 tY 5 .0 0
%0l U3 US
D-S 7?
77 . 75
75 Po -
<P~Co
<9 4<p Po
I fa
NOTES
Qaalitr
tecAs O ^rty A u m a oltx*t lor thtasi I csnily th-i nop la a n t (rem *p*d protocol accurnd io- iiw mpKiwns (wJms MteAMM ~Hd teo~) and tf-t Ih i te s -rcwataiy stet li m i m *t tM f rlonrad by CBL
'/> //!
TEST I D.: ~ i fl-ft
D-310
US00001491
INITIAL (ARRIVAL) EFFLUENT CHARACTERISTICS:
Sample Date
Temp. (C) | 3 , S~ ' |
Conduct. (uMHOS) | *i%C> |
Salinity (g/kg) | l
|
'
| | | |
| ". 1 1 1
|
1 `1
1
|
1!
i
| III
pH I %, 0 I I
1
Diss. Oxygen (mg/1) | ( <Q. /
| 11
Tot. Chlorine (mg/1) | <-O L I I 1 1 1 1
NH3-N (mg/1) ) . Ql 1 ! 1 I l 1
Hard (mg/1 CaC03) j /C? | I I 1 I 1 1
AlUn(mg(l.eaC03) 1 5 5 7 - | | | 1 . 1
1
Color/Appearance1 | C X
1
. Initial?
| 0- f3
I
11
1 *1
1
1 C-Clear O-Opaquc Y-Yellow B-Brown BI-Black G-Grecn T-Tuitid SI -Solids (S-Slight M-Moderate V-Veiy Much)
EFFLUENT ADJUSTMENT/POST-ADJUSTMENT MEASUREMENTS:
ACUTE
Date
|_ |
1 1'
1 1 Ilf 3 / 3 /1
Temp. (C) | ` ' |
-1
1
1
1
I
IU ) ,f
Salinity (g/kg) |
|
1 1 1 1 I Il 3 b
D O. (mg/1) | | 1 1 1 1 I II W
Aeration Rate -| | 1 1 1 1 I II -
Aeration Time (min) |
|
1 1 1 1 ! II _
Adjust. D.O. (mg/l) |
|
1 1 1 1 I II -
PH | I 1 1 1 1 I II 5 W
Tot Chlorine (mg/1) | | 1 1 1 1 I II
Initials
|
|
1111
II c-fl
DILUTION WATER CHARACTERISTICS: DMW:
HWM:\_/
ACUTE
.Date
|
| .... 1 1 1 1
Temp. (C) |
|
1111
Conduct. (uMHOS) |
|
1111
Salinity (g/kg) | pH I
| I
1 1 1` 1
1|
1
Diss. Oxygen (mg/1) |
|
1
1
Hard (mg/1 CaC03) |
|
|
WsOtl HfVrA .
- II a n
II t . i V
II
II m Q
Alkln (mg/1 CaC03) |
Initials
|
| |
1 1 1 1` I 11 1
HM___ _ II CrfS
1E F F L U E N T ID: 1
* n <1/
_ TEST ID: T r t 3 4
D-311
US00001492
Toxicity Test Sample Chain of Custody (Please complete all information)
Facility_ Q \ c ? i P p M l n t o n ^ i v p x ^ i - k t ________
Address.- h t o r - f e l K V A
0
County_____ -- _______ _ NPDES#___________ _______
Pipe/Outfall/Location Instream Waste Cone
Sample'collected by (print&sign) v T l b S Affiliation FX)&- & W . Ea q ,,1r \ U
Type of sample ^ (Grab):
DD aattee 1\i%/ o/ 0i \//Wc\ '1 TT'imm/e*. S : 5 Jw* Yn
^
---------(Composite): From Date_______ Timc_
To Date___
Time
Subsamples comprising composite:
.
Number__________ _ Frequency of collection______ `Temperature of sample in sample collection device 0
Volume
Final temperature of effluent at sample collection poinO
`Is sample collection device chilled?
Is sample packed on ice for shipment?
Tt is required that all samples remain at 0 --4C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency (Do not freeze!)
Is the sample chlorine ? ? _ * _ _ dechlorinated? E O If so, h o w ? * ~ /a .________________
Permit with interim chlorine limit? -
If yes - limit fmg/L) ~~_______________
Field pH-- ___
Field Total Residual Chlorine___________
Comments/'Sample description. A S ' C e n j r o t w/ cZ h o u r O JL raM & n
Type of test(s) to be performed (Specify organisms)
,hr;<rVp . r\j>X\V<iCX
Method of shipment T*)P `ilVflr
Q SL
r*.n.mn ti &. Sign Names
1 , Relinquished bv.EV.
Date IZ/Q2-/A7
Received b y D a t e l2 - h h ~ 7
Relinquished b
y Date ^ < 71
00 Time_jf Time < 9 7 /T Time ( O ^ n
RCj ei^Cd
_______ Date / ^ / d - f *9 Tune />
-# lS?J
D-312
US00001493
Print & Sign Names
.
s
Relinquished by_______________________ _ Date___ ___________ Time----------- :
Received by ^ ^ o f c --
'_____ Date--
Ti me------------
Relinquished byg ^ ^ - --
----- Date.VTV ~~C/-Z - Time_^----- .--
Received bv Q w > -- ^ 9 (\ rrt f a>_____ Date--Ia
II-- Time--! fo
D-313
US00001494
D-314
US00001495
Coastal Bioanalysts Inc.
O D U /O cean a Acute Effluent Toxicity Tests
M ysid o p sis bahia & C yp rin o d o n va rieg a tu s
Sample I D. A2 Contract No. N00187-93-D-9748
Delivery Order No. 0235 J.O. No? 1912290, Item No. 0002BJ, 0002BH
PWCNo. 1390 12/1/97 1725 Sample No. JRA97-13672
Submitted to: Ms. Merrill Anderson-Ashcraft
Navy Public Works Center Code 930, Building Z-140
Norfolk, VA 23511
By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062
(804) 642-0168
D-315
US00001496
O12D.1I*9O7CSEaAmNplAe 9S7a-m13p6le72ID.TAe2st ID: JRA97162
Coastal Bioanalysts Inc.
I. TEST RESULTS
Table 1. Summary Acute Test Results
% Effluent Concentration
0
6.3
.-
l T fS '^ E S C C T T ______ 25.0 . . . _____ ___ -
50.0 100
Mean (%) Organism Survival
M. baha
C. variegatus
2 48-h
24-1 48-h
100 100
100 100
100 TOO
100 100
100 100
100 100
. ......... _L0Q._ ; ioo
100 100
.. ! '
...... 100 100
100 100
100 100
100 100
48-h LC50: 95% Confidence Limits: Calculation Method: ' Toxic Units (T.U.):
>100% N/A N/A <1.00
'
>100% N/A N/A <1.00
Acute Test Information:
.T rA 't
Test Type: 'End'Point: " " " ` ' Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
M. bahia Test
48-h Static
Survival
12/2/97 1640
"
12/4/97 1545 !
Chesapeake Cultures, Inc.
12/2/97
20 g/kg
19.0-20.7C
No
N/A
C variegatus Test
48-h Static
Survival
---
12/2/97 1635
12/4/97 1540
Aquatic Bio Systems, Inc.
11/30/97
20 g/kg
19.0-20.7C
No
N/A
Aeration necessary when D O concentration <4.0 mg/1.
D-316
US00001497
O!ZDI19O7CSTa.AmNplAe9S7a-m13p6le72ID.TAes2t ID: JR.A97162
Coastal Bioanalysts Inc.
Sample Collection Dare/Time
12/1/97 1725
Sample Use Dates/T tines
12/2/97 1640/16353
Aeration2 Rate/Time
n/a
Pretreatment/ A djustm e nt
HWM Salts
-Aeration often necessary to reduce sample oxygen concentration to 100% saturation after wanning. 3A/. bahia/C. variegatus test time.
Table 2. Efficient and Dilution W ater4 Chemical & Physical Characteristics (Mean Value (Standard Deviation))
Parameter
Temperature (C)
Salinity (g/kg)
Conductivity (uMHOS/cm)
pH
Dissolved Oxygen (mg/1)
Total Hardness (mg/1 as CaCOj)
Alkalinity (mg/1 as CaC03)
Total Chlorine (mg/1)
n h 3-n (mg/1)
Initial Effluent
2.7 (...)
1
( ...)
990 (-)
8.05 (-)
11.9 (-)
146 (-)
277 (-)
<D.L. (-)
1.2 (-)
Adjusted Effluent 20.8 (--) 20
( ...)
N/A
8.07 <-> 7.9 (-) N/A
N/A
N.D.
N.D.
Dilution W ater 20.4 (-) 20 (-) N/A
8.07 (-) 7.9 (-) N/A
N/A
N.D.
N.D.
4El!lucnt parameters measured upon receipt of sample in lab and after adjustment on day of use. Dilution water (Wimex hw-Marinemix in ASTM Type I deionized water) values measured after preparation of diluent on day of use.
D-317
US00001498
O12DI1'9O7CSFa.AmNpAle9S7a-m13p6le72I.D.TAo2st ID: JRA97162
n . W ATER QUALITY
Coastal Bioanalysts Inc.
Parameter
Control
Temperature (C)
20.4 (0.1)
.0 .0 . n ` : 7.9
(mg/1)
(0.1)
PH 8.01 (0.07)
Salinity (gAg)
_
21 0)
Table 3. Water Quality Summary
M. baha Test
Mean Value (Standard Deviation)
6J%
20.4 (0.2)
U JA
20.3 (0.2)
25%
20.4 (0.1)
50%
20.4 (0.2)
7.9 (0)
8.01 (0.06)
20 (0)
7.9 (0)
8.00 (0 08)
20 (0)
7.8 (0.1)
8.05 (0 06)
20 (0)
7.7 (0.2)
8.12 (0.03)
20 (0)
Parameter
Teihperathie' ; (Q DO. (mg/1)
pH
Salinity (gAg)
Control
20.4 (0.1)
7.9 (0.1)
8.02 (0.08)
21 (1)
C. variegatus Test
Mean Value (Standard Deviation)
6.3%
20.4 (0.1)
7.9 ()
8.03 (0.05)
20 (0)
12.5%
20.4 (0.1)
7.9 (0.1)
8.03 (0.08)
20 (0)
25%
20.4 (0.1)
" 7.9 (0.1)
8.08 (0.07)
20 (0)
20.4 (0.2)
7.8 (0.1)
8.14 (0.05)
20 (0)
100% 20.4 (0.3) 7.7 (0.2) 8.20 (0.12) 20 (0)
100% 20.5 (0.3) 7.8 (0.1) 8.19 (0.10) 20 (0)
D-318
US00001499
O D I' O CEA NA Sample I D. A2 12 I 9 7 Sample 97-13672 T en ID: JRA97162
III. OUTFALL DATA SUMMARY
Coastal Bioanalysts Inc.
T est P ate
9/30/97 11/3/97 11/25/97 12/2/97*
Table 4. Cumulative Data Summary - Acute & Chrome Tests (Tests conducted on this outfall by CBI)
Invertebrate
Acute Tests
Chronic Tests
LC50 T-U.
NOEC U L
>100% >100% >100% >100%
<1.00 <LQ0 <1.00 <1.00
N/A N/A N/A N /A N/A N /A N/A N/A
V ertebrate
Acute Tests
Chronic Tests
L C 50 T.U.
NOF.C T.U .
>100% <1.00 >100% <1.00 >100% <1.00 >100% <1.00
N/A N /A N/A N/A N/A N/A N/A N/A
` Results submined with this report
IV. QUALITY ASSURANCE
M. b a h ia - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
C. variegatu s - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
Chesapeake Cultures, Inc. (same batch as test) 12/2/97-12/4/97 KC1 (Sigma "Ultra" Lot#76H10391) 343, 490, 700, 1000 mg/1 100% 606.9 mg/1 (578.6-636.7) 608.2 mg/1 (489.3-727.0)
Aquatic Bio Systems, Inc. (same batch as test) 12/2/97-12/4/97 KC1 (Sigma "Ultra" Lot#76H10391) 432, 720, 1200, 2000 mg/1
100%
1169.7 mg/1 (1044.1-1310.5) 1074.9 mg/1 (890.8-1259.0)
D-319
i
US00001500
CUMULATIVE DATA SUMMARY NPDES Permit #: N/A Oceana - ODU A-2
Date.ofV; InvertbratecCiiv lest -
2/12/97 > 10 0 2/28/97 Invalid 3/12/97 > 10 0 3/20/97 > 10 0 3/26/97 > 10 0 9/30/97 >10 0 11/3/97 >10 0 11/18/97 > 10 0 11/25/97 > 10 0 12/2/97 > 10 0 _
Vertebrates.-; -,
>100 >100 >100 >100 >100 >100 >100 >100 >100 >100
_ Invertebrate^'.:,
Vertbrale--' --
N O E G % ^ ^ . ^ NOEC %
Supplement D-320
US00001501
C O A S T A L B IO A N A LY S T S . IN C . NPDES STATIC AC U TE /S A LTW A TE R
(Form AW B4)
IglfU ** Control
5.25 2.5 2S 50 100
1.0.
C- C-2 M 1-2 2-1 22 3. 3-2 4-1 4-2 51 SI
rvf.
Tim*
0*y T 1U* 1 a> te
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t0
lo
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tb
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10
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1o o
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rc g re < L -
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I 2 - j ' 2 , ( <!\ /~)_
AclifTi*ofiwstar HWM K S N
Odsr.
Shinty:.
JLxiiiTvttoo Trrp- (fing*):_____
l^ .o
ui n o n .
jFrrdnq pywr to toot: Anomo
Ji Othor.---------- -.-----
F-Mr^ Amng t**t: Not tad n
AltMT
-
/ f
-
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Othor
Chorror ino: 300 rrt ^
500 ni
1000 iH Otha:
Tort volar: 200rrf ^
250 n4
500 rd Othor
Na d ir : 2 .
Ptotoportott 1EIJO 3 Othar._____ _ o.-..tooodtrat
"4 "4
ST UP. . . . 1 2 - f
T o m W a a A O d j l ^ S l ----------
T u n . A m * A d * d _ l k l ____________ S ^ U p ftr C r f f J L
INSTRUMENTATIONUSEO:
A lStam TNanmmatar Nuntec.
TS 0.0. Motor 1 (StB01447S)
g &ei Ch<oio Motor (D95OE6UJ Sonor R16GP Solere (40020083)
O Y3 SCI 33 Motor [H80H782)
1Q j i r a 0.0. Mote 2 (SN91A028328) Q Carini240 * Motor (S 2M )
STPortopH2 Motort*4230)
SpatatATCMnetamur 1A3M)
Other
Doy 0
Doy 1
Doy 2
C O o.V
i >717,5
T I r > V 2 0 . i 90 -5 .
E 2 0i> r Z O . 1
U
P
3 Dn.r
2-0 - 3 . <?0.3
4 ao- 4 90-3
5 O .? ac> 2-- <9> 3
C & b * g -.o i 77>
] s\ ? 7-f?
2 6 . e * $ F o o 7 ?P-
3 g ./o
7-?f
4 tf. T
5
llV
^G. O S '
C X-t-f---- 8 - 0 ..n 1-- t 7. ? 7 1?
0.0. 2 X . 4
(nVO
3M 4
5 > .f
S C
7.7 -7 -.-- 7 7 ` _ 2 2 ___
7 t 7-2--
/ C-'-- 7- 7
O 3\
A 1 r3 /> l 2 rO S
I
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20 ~ 2O
70 -
fln l-- ___
<90
(sfa)
4 5
cITaMl* L9 bals:
at>
i O ft--
2 C
20
3 5iO
9o i
fe -
NOTES:
TESTI.D.: ~f> *
D-321
US00001502
COANT.U. U10,\NALYSTS. INC. NPDES STA TIC A C im S A L T W A T
(Komi ACV4X)
1Elil<it I.D. Dfaliyva
#DU-i2
Contra^ C-l 1o C-2 l r>
Io IO
M 1o
0
6.25 1-2 t _ _ L*____
2 10
1d
12.5 22 1 _ _ U ____
3) 1c>
10
25 3-2 1o
10
4) f o
in ___
50 4-2 J.D - t-b___
VI 1 0 to 5-2 10
1 o___ (Q___
Oteor**T Pft
P?> .-
Tum: IfriQ--- \ t t Q -
FSwinvat)v%W 1DO |0 o
100
1oo ( 00 ( oO
w*
ca /> \r
Acctomtian w *iar HWU ASW 0 OtWT
s * "2 - 0
u> 7 ^ o . 7 .
id Oihar
Fm diij (Aiir*j t n l: Mot tad C M . 300n<n T nl vote: 2 3 3 / H n
A/tonM
i Ollar
500 0 250 iW
103 00 E ] Otte:
IH
J3500 8
O tte:_________ 4
te . t e t e _ Q ________
PlBU tem t 161-80 p j i O tte:
STUP- Ou. (2 -/
llr tw n M W d llll^ ^ ' l y l
r e W m r * * < _ L l i a i ^ ------
T a a l n n l l U M l l i "V S
S rtU g o C rR fP ^ P *
iNSIHUMlNIAdONUSiD:
Siwn Tharmfmiar N u lte r r\ f I] TS 0.0. t e m 8 ) (S 8 0 l4 7 5 )
2 c J , &iio04tete(BS5OQS63) 11 tetoni.R 160PB.teco (40020083) O TS SCT 33 t e t . (H6016732]
TS 0.0. t e t e 2 (9C1AQ2632S)
L 3 C n iaq 240 04 t e t e (SM526I)
SPPoiu042tetei8S236O)
( 3 Seno ATC fW te ta n a te (A366)
On 0
0*v 1_________ 0* t ?
c 6-9
? S 5
T1
je; y P0.7>
E hi
2 .*r
y Pc-Y
3 .r j>. ?
4 3& i. D0- PO.Y
5 4 .9 PO- H.
C %.o7. s . o f 23
1 ff.? y
P.77
04
2 ?.&9 9 .o'? 7 1 ? 3 ff. IO S*, fS ? -0
4 t . 01 S . i f S-i
5 &.C ri 9" 77, ^'77
C 2 .9
7.3
1 7 .f
7. 7-- 7 1 -
D.O. 2 7 .7 7 . 8 - 7-t--
3 7.7 7 -1 _ 1 7 - l_
4 7.1 7-Sl _ 7-2 _
5 7M
7-7_ 7 - ? _
S C -SA
% - j i
A
L
t 3A 2 <2tA
H 3 7) a
71 _!___ 7 o S>0 2-0 9o
isW 4 Qr>5 D A
20
20
? g\ >
Racolai / 7 t
Intu* ^ .- _ J 2 _ . A i -
MOTES;
Omkpi a n a ;
mA t udity A i w m ofticar for thit ta il I cafiiy
vynfiet
ih n ilrw i Irwn p ia d preiocol uecwiad &***}iW a ntprctiuni
(W*6S oDwrwi** retad Wow) od th! Ihr tet ccirtWy ta ti m d m i ^i lo f f f d b f Cfll.
ir"
Carmenu
TEST I.D.:
A -^ 7 Ife>.
D-322
1
US00001503
C O A o T a .b i o a n a i .l i u >'-
initiai fA R R I V A L ) EFFLU EN T C H A R A C T E R I S T I C S : -------------------------------------------------
Sample P a te 1 / .3///< )? 1
1 1 '- I I
I
Temp. (C) I Q . ? 1
1 1. I ! I
I
C onduct. (uMHOS)
1 11 III
Salinity (g/kg) | I
l
pH I S . T
Piss. Oxygen (mg/l) | i i , 1
I
Tot Chlorine (mg/1) I H ? C I
I_________I _______ !------------- L------------ !------------II
I I .... I I I
I I .. I |
I
NH3-N (mg/1) I / , H ard /me/1 CaC03) I (V C ,
1 I
___________ !------------------ !----------------- L ---------------------------------
I
I_
i
I
I __
AlWIn (mvA CaC03) 1 V ?-- 1
-
rn lo r/A n D e a ra n c e 1 IC Y
I
1 _____ __!_.______ --!-----------------------------: ' II III
..-
_____ iS------.. ^ ,, .J pi J r : ------ t-T.tL si -Solids (S-Slieht M-Moderaie V-Very Much)
EFFLUENT a r ir s t MENT/POST-ADJUSTMENT MEASUREMEIN 15.-----
ACUTE
Date
I
I
I
I
1
|
1Term) (C ) | 1 I
1|
1Salinity (g/kg) |
11
1|
D 0 (mg/1) 1 1 1
1|
iAeration Rate | 1 1
1|
Aeration Time (min) | 1 1 1 1 |
Adiust D O. (mg/1) I 1 1 1 1 |
LpH |________
I1 I1 I
|
Tot Chlorine (mg/1) | 1 1 1 1 I 1i
Il/Q / J
II 7- / / II II II II $ ' 0 3
il (ri3^
EFFLUENT IP: f? cQ___
.*'3 9O
TEST IP: 5 H -fl fCt) D-323
US00001504
Toxicity Test Sample Chain of Custody (Please complete all information)
Facility j Q l ^ _ V o M in i o n lM \ v e r Q i -kj_______ Address_- P f r r - f e l U V A ^ ^ 0 9
County_ NPDES*
Pipe/Outfall/Location Instream Waste Cone
Sample'collected by (print&sign) O T o S O r V / f f i ^ Affiliation
l7oU
Tvpe of sample *'" (fGrabV):
rDwate _ 1\2Z.//V0 o\ /t</Q\ 1*7 Time 5
^
.(Composite): From Date_______ To Date
Subsamples comprising composite:
Time. Time
Number-- _______ Frequency of collection_______ _ Volume__________
Temperature of sample in sample collection device 0 * 0 /
.
Final temperature of effluent at sample collection point
Is sample collection device chilled?
Is sample packed on ice for shipment? H&S
It is required that all samples remain at 0 - 4C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency (Do not freeze!)
Is the sample chlorinated? A-C dechlorinated? Ho If so, how?
____________
Permit with interim chlorine limit?
If yes - limit (mg/L)_
Pieid pH----_______
Field Total Residual Chlorine
Comments/Sample description_ A Z -
* / jj
cultoM o^
Type of test(s) to be performed
6 g.hrjflVp . C r ij ^ V i Y l O d C X ] ______
(Specify organisms) V a t - f e ^ f l 'h l g ____________________________
LVfethod of shipment OP
\ M jD2-Ifiu .
--------- Sign Names
M< ^ 0 0 c / r *
1 . Relinquished bv Dr. M
> Dat e - / 0 i / ^ 7 Time {0- O Q
Received by, Relinquished by ^ ,,9
Date 7 Qj >z , Date / > / !
Time 7 ?/^ Time
t /$ 7 < 3
R^ ciXed
____ Date ^
Time
_____
FOR REED LAB USE ONLY
J R A # ^ 7 - / % (r,'n < ^ L
Arrival Temperature.
Color________________ _____ Odor
P H _____________
DO (mg/L)_
Conductivity (umhos/cm)
Salinity (ppt).
TRC (mg/L). _____ Method
On ice?
JN
D-324
3v 5/16/94
Print & Sign Names
Relinquished by___________________ Date____________ Time_______
Received byL
Date 1 3 . - ^ - ^ Time_______
Relinquished byg&fcfc.-- -V---a ___ Date fa- ~a> - ~7 Time_______
Received bv ^
t.6 ______ Date l a - ~ > - 0~7 Time ! ix r x o
D-325
US00001506
D-326
US00001507
Coastal Bioanalysts Inc.
ODU/Oceana Acute Effluent Toxicity Tests
M y sid o p sis bah ia & C yprin odon va rieg a tu s
Sample I.D. Al Contract No. N00187-93-D-9748
Delivery Order No. 0235 J.O. No. 1912290, Item No. 0002BJ, 0002BH
PWCNo. 1389 12/1/97 1925 Sample No. JRA97-13671
Submined to: Ms. Merrill Anderson-Ashcraft
Navy Public Works Center Code 930, Building Z-140
Norfolk, VA 23511
By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062
(804) 642-0168
D-327
US00001508
ODI."OCEANA Sample I D. AI 12 .1 97 Sample 97-13671 Tesi ID: JR A9716 1
Coastal Bioanalysts Inc.
I. TEST RESULTS
% Effluent Concentration
0
6.3
12.5
25.0
50.0
100
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acute Test Results
Mean (%) Organism Survival
M . bahia
C. variegatus
24-h 48-h 100 100
2h
100
4M i
100
100 100
100 100
100 100
100 100
100 100
100 100
100 100
100 100
100 100
90 90
>100% N/A N/A <1.00
>100% N/A N/A <1.00
Acute Test Information:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
M. bahia Test
48-h Static
Survival
12/2/97 1630
*
12/4/97 1550
Chesapeake Cultures, Inc.
12/2/97
20.g/kg
19.0-20.7C
No
N/A
C variegatus Test 48-h Static Survival 12/2/97 1630 12/4/97 1555 Aquatic Bio Systems, Inc. 11/30/97 20 g/kg 19.0-20.7C No N/A
Aeration necessary when D.O. concentration <4.0 mg/1.
i
D-328
US00001509
O p t* O C K -\N .\ Sample 1.13. M oihcr I.ujuor I ! 24.97 Sam ple 97-13440 Test ID: JR A 97I52
II. WATER QUALITY
Coastal Bioanalysts Ine.
Parameter Temperature
(C ) DO. (mg/1) pH
Salinity (g/kg)
Parameter Temperature
(C) DO. (mg/1) pH
Salinity (gftg)
C o n tro l
20.4 (0.3)
7.9 (0.1)
8.04 (0.23)
20 (0)
Table 3. Water Quality Summary
M . baha Test
Mean Value (Standard Deviation)
12.5%
25%
50%
20.4 (0.2)
20.4 (0.2)
20.4 (0.1)
20.3 (0.2)
7.8 (0.1)
8.06 (0.19)
7.8 (0.2)
8.10 (0.14)
7.8 (0.1)
8.13 (0.09)
7.7 (0.2)
8.20 (0.01)
20 20 19 19 (0) (0) (0) (0)
100%
20.2 (0.2)
7.7 (0.3)
8.26 (0.08)
18 (0)
Control
20.3 (0-3)
7.9 (0.1)
8.09 (0.18)
20 (0)
C. variegatusTcst
Mean Value (Standard Deviation)
6 3%
12.2%
25%
50%
20.3 (0.2)
20.2 (0.2)
20.2 (0.2)
20.2 (0.2)
7.9 (0.1)
8.08 (0.16)
7.8 (0.1)
8.11 (0.12)
7.8 (0.1)
8.15 (0.06)
7.7 (0.2)
8.20 (0.02)
20 20 19 19 (0) (0) (0) (0)
100%
20.0 (0.1)
7.7 (0.2)
8.26 (0.08)
18 (0)
D-329
US00001510
i
O D I S a m p l e I.D. Mother Liquor ' ll'2-}'97 Sample 97-13440 Tost ID: JRA 97152
Coastal Bioanalysts Inc.
Sample Collection Date/Time
11/24/97 0945
Sample Use Daies/limss
11/25/97 1505/1,5153
Aeration2 Rate/Time
n/a
Pretreatment/ Adjustment
HWM Salts
2Aeration often necessary to reduce sample oxygen concentration to 100% saturation after warming. 3.1/. bahitv'C. variegatus test time.
Table 2. Effluent and Dilution Water 4 Chemical & Physical Characteristics (Mean Value (Standard Deviation))
Parameter
Temperature (C)
Salinity (gA)
Conductivity (uMHOS/cm)
PH
Dissolved Oxygen (mg/1)
Total Hardness (mg/1 as CaCOj)
Alkalinity (mg/1 as CaCOj)
Total Chlorine (mg/1)
n h 3-n (mg/1)
Initial EfflueDt
1.0 (-)
N/A
1406 (-)
8.16 (-)
10.9 (-)
104 (-)
139 (-)
<D.L. (-)
N/A
Adjusted Effluent 20.0 (-) 18 (-) N/A
8.15 (-) 7.9 (-) fU A
N/A
N.D.
N.D.
Dilution W ater 20.5 (-) 20 (...) N/A
8.27 (--) 7.9 (-) N/A
N/A
N.D.
N.D.
4EOTucnt parameters measured upon receipt of sample in lab and after adjustment on day of use. Dilution water (Wimex hw-Marinemix in ASTM Type I deionized water) values measured after preparation of diluent on day of use.
D-330
US00001511
()l> rD O -A N A Sample I.D. Mother Liquor 11 24 *^7 Sample V7-I3*Wl> Tesi ID: JR.\y7152
Coastal Bioanalysts Inc.
1. TEST RESULTS
% Effluent Concentration.
0 6.3 12.5 25.0 50.0 100
.
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acute Test Results
Mean (%) Organism Survival
M . bahia
24zh it 100 100 100 100
4M 100 95 95 90 80
100 55
C. variegatus
2 M 48-h 100 100 100 100 100 100 100 100 100 100 100 100
> 100% N/A N/A < 1.00
>100% N/A N/A < 1.00
Acute Test Information:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
M . bahia Test 48-h Static Survival 11/25/97 1505 11/27/97 1530 Chesapeake Cultures, Inc. 11/25/97
2 0 g/kg 18.9-20.8C
No N/A
C variegatus Test 48-h Static Survival 11/25/97 1515 11/27/97 1540 Aquatic Bio Systems, Inc.
11/22/97 20 g/kg 18.9-20.8C
No N/A -
D-331
US00001512
US00001513
Coasta] Bioanalysts Inc.
ODU/Oceana Acute Effluent Toxicity Tests
M ysid o p sis bah ia & C yp rin o d o n va rieg a tu s
Sample I.D. Mother Liquor Contract No. N00187-93-D-9748 ' Delivery Order No. 0235 J O. No. 1912290, Item No. 0002BJ, 0002BH
PWCNo. 1379 11/24/97 0945 Sample No. JRA97-13440
Submitted to: Ms. Merrill Anderson-Ashcraft
Navy Public Works Center Code 930, Building Z-140
Norfolk, VA 23511
Jt
By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062
(804) 642-0168
D-333
US00001514
D-334
US00001515
A PPEN D IX D-6 BNR Inhibition Pass-Through Acute Toxicity Test Results
480 ppm A F F F P re tre a te d w ith F en to n 's R eag en t
D-335
US00001516
D-33f>
US00001517
Toxicity Test Sample Chain of Custody (Please complete ail information)
Facility-- Q \ c L D p t m O l O n
Address_- o r . V A f t z > p c \
J
County__ NPDES#
Pipe/Outfall/Location . Instream Waste Cone
Sample collected by (print&sign)
Affiliation H D \k B tf J
Type of sample_>^_(Grab):
Date_ \l Zz //o0 i\ / <q W7 TTiimmee,
.(Composite): From Date ______ Time
In U ^
T Date____ _______Time Subsamples comprising composite:
NujPber------------ ----- Frequency of collection._______ _ Volume_________ Temperature of sample in sample collection device 0 * Q s
Final temperature of effluent at sample collection point
C-*
Is sample collection device chilled?_\j6 S is sample packed on ice for shipment?
`It is required that all samples remain at 0 - 4C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency (Do not freeze!)
Is the sample chlorinated? >V3
dechlorinated? b-O If so, how?_ v f c .
Permit with interim chlorine limit? -- If yes - limit (mg/L)__ ;
Field pH----Z . A O
Field To[al Residua] chlorine
Comments/Sample description_
Type of test(s) to be performed (Specify organisms) _ - V a t r e a a i u . G
Method of shipment T)P fa C T ^ \7 ty oD rf yt kk.
. C m p r in o d o n J
"
~
W n t & Sign Names
Q Qo0if^
Relinquished b y j k
f l j e n - UnA& Date t t / O l / T 7 Time
Received b y X totj U t f + y
Relinquished by__g?
f p p ^ Date_ ^ % ^ 7
Received by ~c~Z-4A-J~-. , , , i
~ - A ^f~,
Time 0 7 /.< T " T*i--me. ao ^ Time / o ' S o
FOR REED LAB USE ONLY
JRA#______ ____________ Arrival Temperature ______
Color _________ _
_____ Odor_____
pH----------:-- D 0 (mg/L)---------- Conductivity (pmhos/cm). Salinity (ppt)----------- TRC (mg/L)___:___ Method________ "
On ice? . SoHds___ _____ C
D-337
Sev 5/16/94
US00001518
US00001519
CHAIN-OF-CUSTODY RECORD
& ANALYSIS REQUEST FORM
PWC ENVIRONMENTAL LABORATORY CODE 930 BLDG Z-140 9742 MARYLAND A VENUE 1 NORFOLK, VA 23 5 1 1 - 3 0 9 5 PH: (757)445-8851 FA X :
01 U) U) 00
CONTINUATION SHEET SHEET __ OF _^L
Toxicity Test Sample Chain of Custody (Please complete all information)
Facility--
D n W o iO n
Address__ l o r J p \ VL V A , 9 Z
~~J
County_ NPDES#_--------------------- -----------
Pipe/OutfalL/Location mInstre3m Waste Cone ____________
Sample-collated by ( p r i o t t o i g n U f f i S P ^ ^ AffilWon OOH E r i i T S ^ T T u
Type of sample.!^--(Grab):
D.te I3./P1 / < \ 1 Time S T s j P m ' 3
-------- (Composite): From Date______ _ Time
To Date------- _____ Time___________ Subsamples comprising composite:
m Number--------- ------ Frequency of collection Temperature of sample in sample collection device O * 0 / Final temperature of effluent at sample collection point
Volume.
? sam ple collection device c h i l l e d ? ^ . Is sam ple packed on ice to , shipm ent'
It ,s required that all samples remain at 0 - 4*C during collection period and shipSnmt fo7
a a to be accepted by the appropriate Regulatory Agency (Do not freeze') Is the sample chlorinated?_A___ dechlorinated? h o if so, how?
Permit with interim chlorine limit?------If yes - limit (mg/L) -
Field pH---- $-1^ 0r - - ----------------------~ ------
Fr iiceilud T1oUtt:al Residual Chlorine
Comments/Sample description B Z
' ~~
:
Type of test(s) to be performed MvtetgC aV r.vip
(Specify organn iissmm ss))
V a n e ^ q -fu c :
Method of shipment DP ftVOT^ Yy hm \ / s h J
Vnnt & ,
P.rupn n v d c x i
J -
.Relinquished by. Received by.
----- --; -y Date A ^ / j / T )
Time_____ _ Time ( O f / S " Time />*/?) Time ^
jW S 73
JRA#___________ __ Color_______________
FOR REED LAB USE ONLY Arrival Temperature_____ _ ____ Odor.___
pH---------:-- DO ( m S /L)----------- Conductivity (umbos/cm)
Salinity (ppt)---------- TRC (m g/L)_____ Method
"
On ice? . Solids @_____ C
D-339
a c t 5/16/04
US00001520
- icity Test Sample Chain of Custody (Please complete all information)
Facility_
D n i y i o fo n
I r l
,
Address. J ^ o r f e ' V V A
O'
Countv NPDES#.
^
--
Pipe/Outfall/Location ----------- Instream Waste Cone
Type of sample_lC_(Grab):
Date \7 -/O X / q ~7
.(Composite): From Date.
To Date Subsamples comprising composite:
Time. Time. Time
Number------------ ___ Frequency of collection,____ Temperature of sample in sample collection device O ' t ,
Volume.
Final temperature of effluent at sample collection point
2 ? C,
Is sample collection device chilled'? MftS u
,.
!(. - . . .
ls sarnple packed on ice for shipment7
It is required that all samples remain at 0 dr ,w - ,, -
:r r
* ,he " m p l' cW n n a , e d ? ^ C _ d e c h l o r i n a K d ? J a I f s,, , h o w ?
Permit with interim chlorine limit?
II yes - limit (mg/L)_
Field p H _ J L ^ c_
Field Total Residual Chlorine
Comments/Sample description f%>t
^
^
Q -----
vpe Of test(s) to be p erfo rm ed _ M ^^t^
(Specify organisms)
Vrt
Method of shipment OP liv e r rt
Q fth
C rU P n Y tQ g ffC tt J
' ------- -------
1 Relinquished
Pfint & Sign Names Date f r / g z / 4 7
Ot
Time
2. Relinquished bv A
R c t ,-v c d b v
\
^ S l l / L. ~ l l me--
\
-------_ _ D a t e T i m e / g f e
JRArr.
Color.
PH___
Salinity (ppt).
FOR REED LAB USE ONLY
Arrival Temperature________ _
_____ Odor DO (mg/L) ------ Conductivity (pmhos/cm).
TRC (mg/L)____ _ Method
On ice? . Solids^ ___
<2*/Q
D-340
Scv i/1 5 /9 4
Toxicity Test Sample Chain of Custody (Please complete all information)
facility-- C A c L D o v t i o i o n
Address
A dP35<c>q
County_____ -- NPDES#
Pipe/Outfall/Location Instream Waste Cone
Sample collected by ( p r in t & s i g n ) _ J ^ O n ^ ^ ! 5 C i _ Affiliation fX>VL E W
Type of s a m p le d (Grab):
Date \ Z / 0 \ / e \ 1 Time $ * 5 iY [
1n U ^
-------- (Composite): From Date_____ _ Time _____
To Date__________ Time Subsamples comprising composite: * y ^
_____
Number-----------.----- Frequency of collection. 'Temperature of sample in sample collection device O 0Gs
Volume
Final temperature of effluent at sample collection pointI & E & C s
I s sample collection device chilled?^CS_ Is sample packed on ice for shipment? V f A
It is required that all samples remain at 0 - 4C during collection period and shipment for data to be accepted by the appropriate Regulatory Agency (Do not freeze!)
Is the sample chlorinated?-- W
dechlorinated? h o If so, how? ^ /(K
Permit ^interim chlorine limit?____ - __ i f ye s - limit fmg/L) ~~
Field ----- Field Total Residual Chlorine ' _____
Comments/Sample description A S ' C & y i t T e
d j kxDUT' C U L ttL ^ iS rf
~
Type of test(s) to be performed A \ < i\ct h r t* V p , CrVjp T i Y l V d C X l
(Specify organliissmmss)) _ VV #f lKr f e ^ q - f u g
J
Method of shipment DP
\>i\ O lili
r--n--n--t--&- SSigi n Names
0 0 CUM.
1 . Relinquished by Or,
Date I Z / 0 2 ./ 1 7 Time O'O&tXJht
Received by Relinquished by
Received bv- 3 ^
Dat e / A A ?~7
__ D a t e / /
Date. /$-/&-/<7 y
Time 8 9 / X Time f / ? Time />
JRAir_____ _______ ________
Color________________
FOR REED LAB USE ONLY Arrival Temperature_____ __ _____ Odor____
P^ ------ :-- :-- D 0 (mg/L)----------- Conductivity (umhos/cm) Salinity (ppt)---------- TRC (mg/L)______ Method_______ "
On ice? Solids C
D-341
Rev !/:$/?<
US00001522
Toxicity Test Sample Chain of Custody (Please complete all information)
Facility_ - j ^ ^ ^ G j v o t o n I X m v e r c s - h j
Address,
County, NPDES#,
-------------------------- - .
Pipe/Outfall/Location uInrsitream Wwaste Cone
Sample collected by ( p r i n t & s i g n X ^ E S O r ^ g ^
T. pc of sample,*^ (Grab):
Datee., \i/ o x / < \ 1 Time
.(Composite): From DDaattee_, ___ To Date
Subsamples comprising composite:
Time, Time
?
. Number---- ---------- Frequency of collection Temp. erature of s--a-m- rp.l_e in sample tc-oululceLctiijoun odevice oO tv Final temperature of effluent at sample collection point
Volume
IS sample colicction device c h i i l e d i ^ S , is sample packed on ice for shipment* H M
^
d a.! L T
SamP,' S rem ai" a` ' 4"C dUnnS C0,''a i " t* n o d and s h i i ~
e accepte by the appropriate Regulatory Agency (Do not freeze')
Is the sample c h lo rin a te d * ,* ? ------ dechlorinated?.!*. If so how* - / a .
P erm ttw tthm tenm Chlorine limit*.------ If yes - limit (mg/L)_ ~
Ie PH----O -2--
Field Total Residual Chlorine,
'
mm ct Sign Names Relinquished byjf r . MfUeHert-UrAjg Date ] Z / 0 Z / l 7
ReCeiVe<1 Relinquished by
Received byJ & U r * c
Da, t J i h k n Date~ / i f e - , '
Date jJ n
q .p o
Jf L
Time K>* Q M x ~
Time 7 ? /c T ~ r f f S r r t
Time [ n i f - n *
Time 'f o
JRAir, Color. PH___ Salinity (ppt).
FOR REED LAB USE ONLY Arrival Temperature____ '
____ _ Odor_______ DO (mg/L). ----- Conductivity (.umhos/cm),
TRC (mg/L)______ Method
On ice? Solids-
D-342
Se 5/16/94
Facility_ Address
T oxicity Test Sample Chain of Custody (Please complete all information)
P p tv o io n
County__
NPDES#_ ----------------------------------------------------- *
Sample'collected by
Pipe/Outfall/Location uIni osturceuami i j Ww aassttee iCo onnee
Type of sample_. i^_ --(Grab):
Date_ J Z / P t / q 7
,,(Composite): From Date
To Date Subsamples comprising composite:
TTiimmee. Time. Time
_ Number---------------- Frequency of collection______ Temperature of sample in sample collection device 0 * 0 /
Volume
Final temperature of effluent at sample collection point_ & h Q ,
is sample collection device c h i l l e d y ^ S . t sample pack5d m icc for shipment,
. ts reqotred th at all sam ples ,, m a m a, 0 - 4"C during collection period and
data to be accepted by the appropriate R egulator, Agency (Do not freeze')
ITsc the sample ca ehLlto___r'ina.tedtA?. ^rvo
/
dechlorinated?_Ko If so, how? ^ /o .
Permit with interim chlorine limit?
If yes - limit (mg/L) ~~
Field pH--
. field Total Residual Chlorine.
Comments/Sample description A t
3
*nni s ig n Names
o. ^
Relinquished byjfr.
Date t t / o z / 4 7 Time tO fflO oU v
Received by---- C^ W t , ^ Relinquished
P a t e . / i / V f 7 ___ Time i . 7 r - p /iS I t _ Timc
Received by^ ' ^ t
---------- D a , T o n e ' AO *A3
J R A # ____________________
Color_________ _______
FOR REED LAB USE ONLY Arrival Temperature____ _ _____ Odor
pfi----------:-- D 0 (m&T.)----------- Conductivity (,umhos/cm)_
Salinity (ppr)_--------- TRC (mg/L)______ Method
"
On ice? Solids
D-343
Rev 5/16/94
US00001524
Toxicity Test Sample Chain of Custody (Please complete all information)
County.
NPDES#------------- ---- ----------- -
Instream Waste Cone
'
^npIricoUcctcdby Type of samplc_ d --(Grab):
A fn iia i^ W & ^ & T T n U Date IZ / o . / q 7 Ti.no l l t o O ^ T ^ ^
--------(Composite): From Date________ Time
Date__----- ,, Subsamples comprising composite:
Time______ _ ~
. - Number-------------- Frequency of collection_____ _ Volume
Temperature of sample in sample collection device Q * C s
"
Final temperature of effluent at sample collection point
Is sample c a t i o n device c h i l l e d ^ Is sample packed ,,,, for
ts required 0 . ell sanrptes remain at 0 - VC during coiicctlnn period o,,d
Ufa 10 be accepted by the appropriate Regulatory Agency (Do not freeze')
Is the sample c h l o r i n a t e d ? ^ ------ d e c h lo r in a te d ? _ te . If so how '
Permit with interim chlorine limit?
If yes - limit (mg/L)_
Field pH--( n -- Field Total Residual Chlorine
Comments/Sample description P p j P A Sq CJ'C,
%
Type of test(s) to be performed_Mvjt< ^V r.'nVp | (Specify organisms) V flriP./afl-h|gr '
Method of shipment DPllVCTrt y?u Q [$ ji
^
~
Sign Names
1. Relinquished byj Jfe grjert-tinajg p ate IZ/0 2./47
Received by_ Relinquished by.
~
- 1 j >a S ^ , oP f
D a te J ^ / 3 / ^ 7
Date /',,
Received by
~ "
00
Time 3, _ Time 0 9 / ~ Time /O <7) Time /S' y'O
_
JRA#_ Color_ PH__ Salinity (ppt)_
for reed lab use only
Arrival Temperature_________
____ Odor____
DO (mg/L)---------- Conductivity (umhos/cm)_
TRC (mg/L)_
Method
On ice? . SoIids-_ @ _____
D-344
Rear 3 /1 6 /? 4
US00001525
CH " 'N -O F -C U S T O D Y R E C O R D
CLIENT INFORMA"'" " '
& ANALYSIS R EQ U EST. R M
COMPANY/COMMANDiQag^A-______CC_ .
PW C ENVIRONMENTAL LABORATORY CODE 9 3 0 BLDG Z - 140
CONTACT: PHONE:
\ k ,V K _______________
EXT:
FAX:
ENVIRONMENTAL
9 7 4 2 MARYLAND A VENUE NORFOLK, VA 2 3 5 1 1 - 3 0 9 5 PH: (7571445-8851 FAX: (757)445-8852
J -O -ft I e! / 1 J . T O
SIGNATURE: oa/ - / PERMIT NO.:
MjABUJ.Sir ' M K
XPU
OK
i r TYPE SAHPUO
HOfSAMPLES
F IE LD R E A D IN G S
PRUHVJUIOM
[ in e i t e m #* S A M P L E N
00a B H Ooa&T | 3 S' 1
S A M P L E ID /L O C A T IO N f | ! * C o a / V t o J
TAJIK STAAT STOP
DATE
TIHE
Y
l^ v r G- X X ,
HATRIX preservative
/COHTAiHERS
A N A L Y S IS
L / f* ' 8/ 0 a s s a y
pH 1 TCHPCRATUR6 OTHER
S-HH
ItfW FIf 0 ]/ lb
?TAAT
__
31? -
\C r u v R c *
STOP
I7M
]
1
Ub 3 ' c _
is n
STAAT
-- ftnur STOP
rm
START
1 -
~T
A S . 4*-
1
1 13 7 z ....... J...... 3 V
\3 ` t t
l P c . f o A r / w r
- STOP
Pit <t:/Yr,r START
O . - p o P fr fi-FFP STOP
_ 0 foA*** START
STOP
M5 nw
1-- --
9>A(,, A S ' C -
29? , ? 9
<_
SJj j s A
j j
73,4.
Ui IT
--------
1`
P ^cH w r*
START STOP
--
MS
1. s - n.............................. ...........................................
o_. i
as- j
--4 i
TVDP
G - GRAB C F . C O M P O S IT E , F L O W C T C O M P O S IT E , T IM E
L -L IQ U ID
S W -S W IP E
S - SOQ
GS -G A S "
m.
SS - S M I S O LID
P -P L A S T IC G L -G LA S S T - TEFLON V -V O A
B -B A G
A -A M B E R (g la s s )
C - C A R TR ID G E
TL T E FLO N LIN E D LID .
H H E X A N E R IN S E D
1 - CO O L TO 4 C 2 -H N 0 3 , pH<2 3 H2S04; pH<2 4 -N aO H , pH >12
5 - HCL, pH<2
6 0 .0 0 8 % N a 2 S 2 0 3 , 4 C
7 - F IE L D F IL T E R .
8- N O N E
tu r n a r o u n d (O .n ): X O
COMMENTS:
(FO R RU SH TURNAROUND STA TE REASO N BELOW )
REGULATION APPLIED:
RCRA ( )
HRSD ( )
SDWA ( )
TSCA ( )
CWA ( ) PHOTO ( )
CAA ( ) OTHER ( )
I SAMPLING/COLLECTION CHARGE:
POSSIBLE SAM PLE H AZAR D S: COMMENTS:
$
SAMPLE DISPOSAL: ( )fcETURN TO CLIENT ( (DISPOSAL BY LAB
ESTHOMBER:
IN IT .:
CONTRACT LAB;
DATE:
. C U S T O M E R IS R E S P O N S IB L E F O R A L L C H A R G E S N E C E S S A R Y FO R TH E P R O C ESSIN G AND A N A L Y S IS OF S A M P L E IS I ALL R U S H S A M P LE S A R E S U B J E C T TO S U R C H A R G E .
CONTRACT NO.(S): '
OFFICIATUSE'ONLY"
"
................
2. S A M P L E S _ R E C E ^ D ^ F T E R 2 :Q 0 P M M O N. - TH U R S. AND 1 :0 0 PM ON F R IDA Y WILL B E P R O C E S SE D THE N E X T B U S IN E S S DA Y 1 7:00 A M - 3 :3 0 PM ).
1
1
? F < ^ L a S L J S E O N L V H T v : ' .
REJECTED! )
REASON:
\
HOLDING TIME(
;? . T : V : - : a L 1'. S M ^ ^ s ' ^ e r .i f i c ' t i n i
yv .v 'v T V -
CONTAINERS! )
IMPROPERLY SAMPLED! )
OTHER!
US00001526
Toxicity Test Sample Chain of Custody (Please complete all information)
Faci!iry_ C A c& . P o m p o n . Un A n d C n cA dr!rd!trvecscs , VPiOO rfX- ^VbolIKv - . v wJ Aa M ' O Z
J
^ ,v
C o u n ty __
P ipe/O utfall/L ocation
NPDES#.
Instream W aste Cone
Sam ple coliected by (print& sign) O J o S e K y ^ ^ - - A ffiliation CPlA &r/ g n ^ jT o b
Type of sample
(Grab):
Date
T im e IO CUr\
^
.(C om posite): From Date. To Date
Subsam ples com prising composite:
Time _ Time
N um ber-- _________ __ Frequency o f co llec tio n . Temperature of sample in sam ple collection device O
V olum e
Final temperature of effluent at sample collection point
Is sa m p le c o lle c tio n d e v ic e c h ille d ?
Is s a m p l e p a c k e d o n i c e f o r s h i p m e n t ?
It is re q u ire d th a t all sa m p le s re m a in a t 0 - 4C d u r in g c o lle c tio n p e rio d a n d s h ip m e n t fo r
d a ta to be ac ce p te d by th e a p p r o p r ia te R e g u la to ry A g e n c y (D o n o t freeze!)
Is the sam ple chlorinated?
dechlorinated?
if so, how ? _____ _______________
Permit w ith interim chlorine lim it?__________ If yes - lim it fm g/L )
- _______________
Field pH--
------
F ield Total Residual C hlorine______________
Comments/Sample description. f e e /
_______________________________ .
Type of test(s) to be perform ed M j S f d f S h f l X i ? . C ijX T f iA o d z X i V Q r i & f f l C h l ?
(Specify organism s)
M ethod of shipment. D e lW e ry h u O Q iA
Print & Sign Names
1. R elinquished by \ / g f f / u j e A loSCV I R~ eceived` by ^ ` " v c ^S y ^ P` ,? R elinquished by ft-In .i l L. . Received by -^ ^ V id t L l l -- J
Date ^ / n / < T 7
Date/J W f 7 D ate f z - n - q y
D ate
f~7
T im e Tim e > f / / ) Time / / /io Time U O V
JRA# Color
_______________ _________________
FOR REED LAB USE ONLY
A rrival T em perature____________
_____ O d o r______________
---------------- DO (m g/L )_________ C onductivity (um hos/cm )_
Salinity (pot)________ T R C '(m g /L )_______ M ethod___________
On ice?
Solids. @_____
D-346
3 (7
US00001527
^IIM-OF-CUSTODY RECORD
& ANALYSIS REQUEST FORM
CONTIK^a TION SHEEi
SHEET
OF
U
) -P*
US00001528
Toxicity Test Sample Chain of Custody (Please complete all information)
Facili ty_ Q l I & r w A Q f l , \ X s S v e r S t j i j Address, i J o t -St% , V A 2 Z Q
\>
County,
Pipe/Outfail/Location
NTDESi
Instream Waste Cone
Sample'collected by (print&sign)
Affiliation 0 [ X (
l& J ?
Type of sample ^ (Grab):
Date, \ 7 - / i p / * n . Time u W ~
a
.(Composite): From Date^_
Time,
To Date___
Time
Subsamples comprising composite:
.
Number__________ _ Frequency of collection_________ Volume,
'Temperature of sample in sample collection device 0 ' C ! s
Final temperature of effluent at sample collection point.
'Is sample collection device chilled?
Is sample packed on ice for shipment?
It is required that all samples remain at 0 - 4C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency (Do not freeze!) .
Is the sample chlorinated?
dechlorinated? && If so, how?_____________ '
Permit with interim chlorine- limit?________ If yes - limit (mg/L)____________________
Field p H F i e l d Total Residual Chlorine_____
Comments/Sample description. M a l f a r \ A j t f t w r - f h * * -
Type of test(s) to be performed M ljQ l'd .
, ( U y p r in n d o n V cu ie< ja .iu S
(Specify organisms) _____________________________________________
Method of shipment. O s ltV e o f b j O D U
Print & Sign Names
.
1. Relinquished by.
\ f o r to r i Date. i y i r / 4 7 Time,
Received by.
Date
Time
( ;Q------
Relinquished bv ftrr\j$!b.i. t )
Date /d-^/V T Time / / . 'oo
Received by.
-k iLJLl-- I______
Date f
~7 Time tto o
{ ^ *7
FOR REED LAB USE ONLY JRAn___________________ Arrival Temperature___________ On ice?________ Color_________________________ Odor________________________ Solids___ pH_________ DO (mg/L)_______ Conductivity (umhos/cm)_______ @_____ C Salinity (ppt)_______ TRC (mg/L)______ Method______________________
D-348
Sev S /5 /7 -*
US00001529
Toxicity Test Sample Chain of Custody (Please complete all information)
Facility. COU Address_:kcr-frlk-.VA-
\^
County.
Pipe/Outfall/Location .
NPDES#.
Instream Waste Cone
Sample collected by (print&sign).
Affiliation._o__p_t_i__B_K__*
Ia M
Type of sam pIe_J_(G rab):
Date- i ^ / f 0 / ^ 7 Time 7 L\~ t?
.(Composite): From Date.
Time.
To Date
Time.
Subsamples comprising composite:
Number________ Frequency of collection_________ Volume__________
"Temperature of sample in sample collection device 0 *0/
Final temperature of effluent at sample collection point.
"Is sample collection device chilled? ^
Is sample packed on ice for shipment?
'It is required that all samples remain at 0 - 4C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency (Do not freeze!)
Is the sample chlorinated?
dechlorinated?
If so, how?___________________ _
Permit with interim chlorine limit?________ [f yes - limit (mg/L)____________________
Field pH % ' "P
Field Total Residual Chlorine.
Comments/Sample description. B f l -
AFFF
Type of test(s) to be performed M ittd tthri*f>. COjerf/uMiri Varr&ictuJ
(Specify organisms) Method of shipment.
by oDli
Print & Sign Names
1 . Relinquished b D a t e
^ Time
Received by. /T b tA lo p ^ V o
Relinquished by
\JHu^ --
Date_ Date fSL -/(. 4' ?
Time /)*7 / ri; Time n <*:>
1^ 0 -
Received byJ n ^ & UJLL--)
Date th~i L~f
Time /C-ac>
FOR REED LAB USE ONLY JRA#________________ Arrival Temperature_________ Color________________ _____ Odor________________ pH_________ DO (m g /L )_ _ _ _ _ Conductivity ((.tmhos/cm). Salinity (pot)_______ TRC (mg/L)______ Method_________
On ice?. Solids.
D-349
Rev r / 1 6 / 9 4
US00001530
Toxicity Test Sample Chain of Custody (Please complete all information)
Facility, D i & P o n w S o n j t f e r & f M
Address, f o O r p l U - . y / A
J
County,
Pipe/Outfall/Location
NPDESf
Instream Waste Cone
Sample'collected by (print&sign).
Affiliation p p n E h y B i C f l a y
Type of sample...^ (Grab):
Date t / x O / V l Time 5
J
-------- (Composite): From Date______ Time.
To Date________ Subsamples comprising composite:
Time
Number__________ _ Frequency of collection. Temperature of sample in sample collection device O
Volume
Final temperature of effluent at sample collection point 3 .* ) * C y
Is sample collection device chilled?
Is sample packed on ice for shipment?
It is required that all samples remain at 0 - 4C during collection period and shipfnent for
data to be accepted by the appropriate Regulatory Agency (Do not freeze!)
Is the sample chlorinated? M dechlor'inated? t o If so, how?_________________ _ Permit with interim chlorine limit?________ If yes - limit (mg/L)_____________________
Field pH-- ` I -- t
Field Total Residual Chlorine___________
Comments/Sample description, 0>2_ - \ z O ^ A f ' F F - S J k p c u r & r
Type of test(s) to be performed M 9 i'A
(Specify organisms)
^
Method of shipment
b lj Q p l\,
^
, (I r v j p n ' n Q t 'C o r l \ t i r f P q C t f u . Q
^
"
Print & Sign Names
I. Relinquished bb yv. d * M j t r J o S o n
Date I * - / * / ? ! Time <4
Received by _ ____ Relinquished bv fL th J&L \J
P a t e _ /^ ./l^ 7
Date 7
T im e _ 2 j7 . ( )
Time //.'c Q
/
Received b
Date i2--i/~f~7
Time
_______
(
J R A # _ ______________ Color ____________
FOR REED LAB USE ONLY Arrival Temperature__________ _____ Odor________________
pN DO (mg/L)_______ Conduct!vity (umhos/cm)_
Salinity (ppt)_______ TRC (mg/L)______ Method_________
On ice? Solids____ @_____ C
D-350
Sev 5/1/94
US00001531
County,
Pipe/Outfall/Location .
NPDES#_
Instream. Waste Cone
Sample'collected by (print&sign). <JToSon
Affiliation. O P U Z n v B n tj U d b
Type of sampled (Grab):
Date
Time
______(Composite): From Date_________ Time.
To Date___________ Time.
Subsamples comprising composite: '
Number________ .__ Frequency of collection_________ Volume.
'Temperature of sample in sample collection device 0 &
Final temperature of effluent at sample collection point.
'rc cam ple collection device chilled? M-6 S Is sample packed on ice for shipment?.
'It is required that all samples remain at 0 - 4C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency (Do not freeze!)
Is the sample chlorinated?
dechlorinated?
If so, how?-------------------------- -
Permit with interim chlorine limit?________ If yes - limit (mg/L)-------- -----------------------
Field pH_
Field Total Residual Chlorine.
D c1bcu JU '
.Type of test(s) to be performed. M j C t d S h r t o i p
V o rte ^ a i i t S
(Specify organisms)
Method of shipment. D e .U ' v e n j b * j o p v L
Print & Sign Names
1 . Relinquished by. Rec:eeived bv (
llo S o n
2
D ate 7 Time Pate \ \ J ( I j , Time
1^ 0
2. Relinquished byv.
m j t e z ______ Date
------ Time--(X2St--_
Received byr.
Date Time il& &
FOR REED LAB USE ONLY
JRA#
Arrival Temperature_________
Color.
_____ Odor___________ '
P H _ ______ DO (mg/L)_______ Conductivity (pmhos/cm). Salinity (ppt)_______ TRC (mg/L)______ Method-------------
On icc?_ Solids____ @_____ c
D-351
3e-/ 5/ 10/94
US00001532
Toxicity Test Sample Chain of Custody (Please complete all information)
Facility. O I C b iH X f tlO Y ) U n i v e r s i t y
A, djdjr_e_ss_
t/A /f<2 tr/^i 'Z
'P u j C
County_ NPDESi
Pipe/Outfall/Location . Instream Waste Cone
Sample'collected by (print&sign).
Affiliation. O P u E h v '& i& L a .b
Tvpe of sample ^ , (Grab):
Date tf- A v / O H Time_ 5 - ^ j m
3
_(Composite): From Date______ Time_
To Date.
Time
Subsamples comprising composite:
Number___________ Frequency of collection. 'Temperature of sample in sample collection device f t *( V
Volume
Final temperature of effluent at sample collection point.
'Is sample collection device chilled? AP.$> Is sample packed on ice for shipment?.
'It is required that all samples remain at 0 - 4C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency (Do not freeze!)
Is the sample chlorinated? M
dechIorinated?_XO_ If so, how?__________________ .
Permit with interim chlorine limit?________ If yes - limit (mg/L) '___________ :_____
Field pH. n p f ]
Field Total Residual ChIorine___________ '
Comments/Sample description. A t - f D e f p g j o & r _________________ ;___________
Type of test(s) to be performed.
S h r im p J r iu p n ' H o J b n V tu rie c r a -b tS
(Specify organisms) Method of shipment. V d f r e n j [n j o p u
J
Print & Sign Names
1 . Relinquished bv\
V fO$C?/t Date ' Z ' / n / Q *7 Time
Received by J Relinquished bv Afa
a S ) r ~ S J t ^ Date ( j f , j Date ; 7 --f/
Time Q 'J [ f0 ._ [ Time / / O Q_____
Received b
AL--/_________ Date t X - H - f --? Time IL&______
FOR REED LAB USE ONLY
JRA#_
Arrival Temperature__________
Color.
_____ Odor________________
PH _
DO (mg/L)_______ Conductivity (umhos/cm)_
Salinity (ppt). _____ TRC (mg/L)______ Method_________
On ice?. Solids. @ ____
D-352
3cv 5/16/9*
. 1
US00001533
Toxicity Test Sample Chain of Custody (Please complete all information)
Facility_ Q \ J L V o n ^ i Y ) \ o r \
Address *
VA 2 3 S O *
_____________
pLvC
County, NPDES#,
Pipe/Outfall/Location. Instream Waste Cone
Sample'collected by (print&sign).
Affiliation. CVlA 6 W .
Type of sample ^ (Grab):
Date_ I ^ - - / Ti me
______(Composite): From Date_________ Time____________
To Date___________ Time____________
Subsamples comprising composite:
<
Number_______ Frequency of collection______________ Volume__________
'Temperature of sample in sample collection device 0 d J
Final temperature of effluent at sample collection point.
Is sample collection device chilled?
Is sample packed on ice for shipment?
It is required that all samples remain at 0 - 4C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency (Do not freeze!)
Is the sample chlorinated? M) dechlorinated? )U> If so, how?__________________
Permit with interim chlorine limit?________ If yes - limit (mg/L).____________________
Field pH % . Q ( f
Field Total Residual Chlorine___________
Comments/Sample description, A Z - d o n j r c r t ________________________________
Type of test(s) to be performed. M u s i t i
V a rfe g c v b i> 8
(Specify organisms) _____________________________________________
Method of shipment
b^j
. Print & Sign Names
1 . Relinquished bv C
yJpSQ /T Date
Time
Received by___/ ? i P - ^ L r Relinquished bv fi mu-L. 1/ Received bv
9 o Date
D^ ate / a
'
Date t x - n - 1-)
Time Q f / t f j -- TTVi--me Z' --/o d' Time UQO
I
FOR REED LAB USE ONLY
JRA"_____________ ._____ Arrival Temperature___________ On ice?_______
Color____________________
Odor________________________ -Solids____
pH_________ DO (mg/L)_______ Conductivity (umhos/cm)_______ @______C
Salinity (ppt)______ : TRC (mg/L)______ Method______________________
D-353
Re*' 5/1/S4
US00001534
Toxicity Test Sample Chain of Custody (Please complete all information)
l
Facility, O l d p o m u o r t t t M V e r g i j u ____________ _______________
A` d-d-re-ss, j j o r f e l k - , VC4
J
--
County_ NPDESr
Pipe/Outfall/Location Instream Waste Cone
\J T o S o ti
PU E hv
.(Composite): From Date.
Time,
To Date Subsamples comprising composite:
Time
Number.__________ _ Frequency of collection______ 'Temperature of sample in sample collection device 0 &
Volume
Final temperature of effluent at sample collection point & 5 . Q * O
"Is sample collection device chilled?
Is sample packed on ice for shipment?
It is required that all samples remain at 0 --4C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency (Do not freeze!)
Is the sample chlorinated? fijQ dechlorinated?
If so, how?__________________
Permit with interim chlorine limit?________ If yes - limit fme/LT ________________
Field pH.
Field Total Residual Chlorine___________
Comments/Sample description. I - i o n t r o Z '
Type of test(s) to be performed M t j S t ' d S h f f / n p .
V a .rt& ?g
(Specify organisms) ___________ ;_______________________ <-/
Method of shipment, D e A twe ns ut hb iut D O U
Print & Sig_n_N__ames
1. Relinquished byA Received by__ i l A
fbr D S& i P.
Date l * - M 7 Time 9
Date Time
1O
Relinquished bv Rhi/A, J \J
Date
Timef f ~ 0 O
Received bv
Date / ^ i M - 7
Time l/2 Q
f SriT7
JRA# Color pH Salinity (ppt)
FOR REED LAB USE ONLY
Arrival Temperature
Odor
DO (mg/L)
Conductivity (umhos/cm)
TRC (me/L'l
Method
On ice? Solids (a) C
D-354
Se*/ 5/15/94
P rint & Sign Nam es
Relinquished by
Date
Received by,.,.
_ Date U> -3 - " t l
Relinauished bv n f r , r , _
S t l :--. Date^.^-
Received bv W v w
c ^ Date
Time Time Time Time
"" fPO
D-355
US00001536
Toxicity Test Sample Chain of Custody (Please complete all information)
Facility_ Q \ c L D o m i n i o n ( M i ^ e r ^ i + u
Address fOor-felVl y\V/AA AP23K5</3) Q.q
U
REED ^
&J 'f
County.
Pipe/Outfall/Location .
NPDES#_
Inscream Waste Cone
Sample collected by fprint&sign) \H b S O y \/f^ ^ v ^ -'> Affiliation f X ) W- & V .
. Ldl?
Type of sample ^ (Grab):
Date 1% / 0 \ / e\ 1 Time / ' h ^ r f \
_____ (Composite): From Date_________ Time ________
To Date___________ Time____________
Subsamples comprising composite: % .
Number______
Frequency of collection.
Volume
'Temperature of sample in sample collection device 0 * 0 /
Final temperature of effluent at sample collection point.
'It sample collection device chilled?
Is sample packed on ice for shipment?
'It is required that all samples remain at 0 - 4C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency (Do not freeze!)
Is the sample chlorinated? tVQ dechlorinated?_0 _ If so, how?_ *y&.____________
Permit with interim chlorine limit? -- If yes - limit (mg/L) ________________
Field pH % . 1i
Field Total Residual Chlorine___________
Comments/Sample description. W y & t U n u e r jV c m & j b r & n t t -
p fo r
Type of test(s) to be performed
(Spe:cify organisms) Vra
___________________
Method of shipment
C A ^ J 0271 \ A .
V nnt & Sign Names
Relinquished by Dr.
" l4/\ ^ Dace X 2-/0 2 -/^ i7
-(fO c w * T im e C ! OQ w**^- ^
Received b^ i t
. Dare l4 gft=Z Time /O f K<T___ - * ^ 7
Relinquished by
Date Time IO f C3___
Keceived ov y & - / ' \3=- -
fa /* n i / / / as--
Time /o *^c`____
FOR REED LAB USE ONLY
JRA# cC 7 '
Arrival Temperature.
Color__________________ _____ Odor____
p H _ _______ DO (mg/L);
Conductivity (umhos/cm)
Salinity (ppi)____ TRC (mg/L). ____ Method
On ice?
D-356
3ev 5/1 /94
US00001537
C O A S T A L B IO A N A L Y S T S INC.
E F F L U E . M , D IL U T IO N W A T c K D.-i.iA ifw ini w.
INITIAL (ARRIVAL) EFFLUENT CHARACTERISTICS:
Sample Date h / / / ? 1
1
1. 1
1
1
Temp. (C) 1 3 M
1
1
1_ 1
1
1
Conduct (uMHOS) k s 'o o 1
1
1
1
1
1
Salinity (g/kg) ! (
!
I
I
I -I
I
pH 1$ . I Q >
Diss. Oxygen (mg/1) I I ) . I
Tot. Chlorine (mg/1) 1L o (~
NH3-N (mg/1) l / . O
Hard (mg/1 CaC03) 1 1 3 1
Alkln (mg/1 CaC03) I D S '^
Color/Appearance1 I C X
Initials
1 t - r 7?
I
1
1 1 1 1
11
1111 1
1111 1
1 11
1 1 1 1 1-
J_ _ _ _ ! L !1 ____
-------------- ------------ -------------
1
1_
1
1
1
1
1
.
Il 1
-Solids fS-Slieht M-Moderate V-Vety Much)
_.
EFFLUENT AnniSTMF.NT/POST-ADJUSTMENT MEASUREMEN is._____
ACUTE
DatP 1 1 1 1 1 1
ll/a /a /ft
Temp. (C) | 1 1 1 1 1
Il D K
Salinity (g/kg) | 1 1 1 1 1 1 II 3 a ...
D O. (mg/1) | 1 1 1 1 1
Il>.f
Aeration Rate
11
1
1 1 II -
Aeration Time (min) | | 1 1 1 1 1 II -
Adjust. D.O. (mg/1) | I 1 1 1 1 1 II ~
dH 1 1 1 1 1
1 iijf.iv -
Tot Chlorine (mg/1) 1 1 1 1 _____ J------------!------- ;---- --------------^J2.
Initials
|
1
1
1
1
-------------
DILUTION WATER CHARACTERISTICS:
DIVTW:
H W M iv/
1
1 II b n AaC/~*Utn1Lc-
Date | 1 1 1 ____ * ------- 1
Il ( a /a /f t
Temp. (C) |_______J______ 1
1 1 1 II On i
Conduct. (uMHOS) 1 1 1 n 1 1 1 II
Salinity (g/kg) | ______ |________1_______ [______ J_____ --J -----------!--------- - ^ ^
-- s--
i
i
i
i
in
1
Diss Oxygen (mg/1) |
I_______ 1________|______ _J________!------------!--------------
Hard (mg/1 CaC03) | | 1 i 1 J -------- J ------------ ^ -----
Alkln (mg/1 CaC03) | Initials
1 1 1_______ !------------!----------- ------------- \ -----
CrQ
EFFLUENT IP ^ jt U
y,iT.
_ TEST IP: T H C j *t ? I U Q D-357
US00001538
Cl IA ST.U . U lo.lN '.U .Y S T S . INC. N P D S iT A T IC A C U T fc/SA LTW A TtR
(Korm AC'V-ia)
1 EMilnr
1.0.
Cl C-?
M
6 f t 1-2
21 12 S 2 2
3-1 32
4-1 50 4-2
61 100 5 2
Qbwrvtr.
I irm:
0lirv1
Oar 2 #U *
lo 1 o '0 ( 0
'o 1 o iu l 0
f 0 10
f 0 10
<0 ( O 10 ( o fO ( o
1 ... _____3 ________
fO (O
(0 1 0
>
so
's * 5
R j ' 5 ______
Final \ Suvwai
l t>0
100
1co lo o
l7 V00
.
Cvorinodon vaneqatus
Souea:
H /tils'
Hitch LUt*Irra:
i U B o /V )
AccliiTwtion w ii*f HWUASW j O
0lhar
Satacy: ""2 --0
<=> u>
pnof to tail An,m` 3 x
_. /d Othar
1/
<Xnr*jtt. ta t !.d Q
Art#**
/d Othar
Ciurraar u i : 300 ^ C-
T o t yoiwr:
^ CD
500 Q
250 tri
1000 ui E
Othar
ri
500 J 2 0U - r _________ mi
trail no. oraafawrn par repbcata: f O
Hi
") ________
ftetopanott l6l.SC CO Othar
Bantonubon lanvfou n o .^ 1
SETUP c u *
( 2 . / 2 - / ' ? ' ! Tam WMa-JOttd ( ^ ^ {i
Tun Aramals Aoted 1 (0 P 0
Sh Up B ^
iNSIHUMlWAilON USD:
AStain Thaorewnatar Nuntwr
(
Y3 0.0. Mataf 1 ! |S*B01 1 f t ) 2 CP D v TM * ( ! ' W960C663)
J Sartonu R16QP BaJanc* (40020093]
Y3 SOT 33 M ai IH801S792)
i TS 0.0. Uaur 2 {90IAO2S329)
L Canar* 740 fHU aur (9 6 2 S I)
SPP o r i U aur (852360)
3 W * > ATCRtfraotamlar (A36fl)
Ottwr
Qtabry Aaataanca:
A* Qit^ifr Aaauvca elite lor ito tvt i Crn.ly that w -**f*\*a rt ^WkMns from aceapitd prctocol uccuiad a*fl*<ilortii mptci* (wdaw otirntm**naiad bakna) and that 11 * u rcuntatr tha mt u t il w k f a n n e d bf C8I.
-- V ` r -\ v = - . /
._ ` 1 i= -'
TEST ID ' i ',1 ft. i1 0
D-358
US00001539
C O A ST,U . BIOANALYSTS. INC. NPDES STATIC ACUTE/SALTWATEB.
(Form AMB4JJ)
\ff Contrat
625 12.5 25 50 100
90o,1
1.0. In*
#D*2 In*
C-l (o C-2 10
10 |0
i-i In . 1 0
1-2 in
tu
21 2-2 10
L___ \0
3-1 10
9
3-2 V q
4-1 10
1o
4-2 10
I0
5-1 //
_ J ------
5-2 / D . ro
trat.~P t<iO SO
H'iO ___
SurvnnJ
\0Co
100 [ t>0 ^0 ( CsO {DO
- 0*0 D*yl 0i*2
C A t>A__ r)o 7 Sb-V
; t D/>
,
E M
2 Oo 3 A 6<
3-Q-t ^ - 3 _ 2o C? >0-0
4 o n y 20. i d*:L--
5 0 D r 90-7- 4*4-0
c 9 1 %cy1 * . a ? fr-Ol 7?7 2 S . 3 ? /i4 _ 7?5?
3 S.3 $-0*1. 4 f.7-0
S f? JH
C
%-51 <T>17. ? _ l __
1 T-.f 7. r SI.%--
D.O. 2 7 -9 7. 7 _2 2 ---
3 7. & . 1 k --
4 7-6 Tfe
5 9--1 7 A 1-S--
S C A d 7 c , -i- 2or
A l
1 ,D O 2 An
le- "_ r zo - g^ -
N3
(sfa) 4 S
itWaf*t:iti 9 Inluti:
Ab AD Ah
) i/43
2c20 2 <7 5
_ l __
1 <"6
motes.
TEST I D.: -I fi ft- V > iko /
D-359
US00001540
CUMULATIVE DATA SUMMARY NPDES Permit #: N/A
, : Oceana -,ODU , .. . ,, Mix L. R.R.
iDate r-_ Inverfebrat^geT -,, , Vertebrate^: ---
Jnyertebrate^%vi~>=: Vertebrate . ~
| 2/12/97 > 10 0
>100
N b E C % -'''';-"
| 2/28/97 Invalid
>100
| 3/12/97 > 10 0
>100
| 3/20/97 > 10 0
>100
3/26/97 >10 0
>100
9/30/97 > 10 0
>ioo
-- ---------------------
11/3/97 > 10 0
>100
11/18/97 > 10 0
>ioo
-------------------------- i-------------------------
11/25/97|> 100 12/2/97 >100
|>00----------------------- -- = >100
...
1
1
Supplement
D-360
i US00001541
O D I'-OCEAN A Sample l.D. M ixed Liquor 1X1 97 Sam ple 97-13669 T esi ID: JR A 97160
III. OUTFALL DATA SUMMARY
Coastal Bioanalvsts Inc.
Test Date
9/30/97 11/3/97 11/25/97 12/2/97*
Table 4. Cumulative Data Summaiy - Acute & Chronic Tests (Tests conducted on this outfall by CBI)
Invertebrate
Acute Tests
Chronic Tests
LC50 T.IJ.
NOEC T.IJ.
Vertebrate
Acute Tests
Chronic Tests
LC50 T.U. NO-EC T.U.
>100% >100% >100% >100%
<1.00 <1.00 <1.00 <1.00
N/A N/A N/A N/A N/A N/A N/A N/A
>100% >100% >100% >100%
<1.00 <1.00 <1.00 <1.00
N/A N/A N/A N/A N/A N/A N/A N/A
Results submitted with this report
IV. QUALITY ASSURANCE
M. bnhia - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
C. variegatus - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
Chesapeake Cultures, Inc. (same batch as test)
12/2/97-12/4/97
KC1 (Sigma "Ultra" Lot#76H10391)
343, 490, 700, 1000 mg/1
'
100%
606.9 mg/1 (578.6-636.7)
608.2 mg/1 (489.^-727.0)
Aquatic Bio Systems, Inc. (same batch as test) 12/2/97-12/4/97 KC1 (Sigma "Ultra" Lot#76H10391) 432, 720, 1200, 2000 mg/1
100% 1169.7 mg/1 (1044.1-1310.5) 1074.9 mg/1 (890.8-1259.0)
- D-361
US00001542
O D I -O CEA NA Sam ple l.D. Mixed Liquor 11 1 97 Sample 97-13669 Tesi ID: JRA97160
II. WATER QUALITY
Coastal Bioanalysts Inc.
Parameter
Temperature (C)
D.O. (mg/1)
pH
Salinity (g/kg)
-
Control
20.4 (0.3)
7.9 (0.1)
7.98 (0.05)
20 (0)
Table 3. Water Quality Summary
M . baha Test
Mean Value (Standard Deviation)
63%
12.5%
25%
50%
20.4 (0.2)
7.8 (0.1)
8 00 (0.03)
20 (0)
20.4 (0.2)
7.7 (0.2)
8.02 (0.03)
20 (0)
20.4 (0.3)
7.7 (0.2)
8.08 (0.01)
20 (0)
20.3 (0.2)
7.7 (0.2)
8.14 (0.06)
20 (0)
100%
20.4 (0.4)
7.6 (0.3)
8.26 (0.11)
20 (0)
Parameter
Temperature (C)
D.O. (mg/1)
pH
Salinity (g/kg)
Control
20.4 (0.2)
7.9 (0.1)
8.02 (0.08)
20 (0)
C. variegatus Test
Mean Value (Standard Deviation)
6.3%
12.5%
25%
50%
20.4 (0.2)
7.8 (0.1)
20.4 (0.2)
7.8 (0.2)
20.4 (0.2)
* 7.8 (0.2)
20.3 (0.3)
7.8 (0.2)
8.05 (0.07)
8.05 (0.07)
8.11 (0.05)
8.15 (0.07)
20 20 20 20 (0) (0) (0) (0)
--
100%
20.4 (0.3)
7.7 (0.3)
8.26 (0.11)
20 (0)
D-362
US00001543
O D t'.O C E A N A Sam ple I.D. M ixed L quor 111-97 Sam ple 97-13669 Test ID: JR A 97160
Sample Collection Date/Time
12/1/97 1330
Sample Use Dates/Times
12/2/97 1600/16003
Aeration2 Rate/Time
n/a
Pretreatment/ Adjustment
HWM Salts
2Aeration often necessary to reduce sample oxygen concentration to 100% saturation after wanning. 3A/. bahicu'C. variegatus test time.
Table 2. Effluent and Dilution Water 4 Chemical & Physical Characteristics (Mean Value (Standard Deviation))
' Jaraifeter
Temperature (C)
Salinity (gAg) -
Conductivity (uMHOS/cm)
pH
Dissolved Oxygen (mg/1)
Total Hardness (mg/1 as C aC 03)
Alkalinity (mg/1 as CaCOj)
Total Chlorine (mg/1)
NHr N (mg/1)
Initial Effluent
3.4 (-)
1
(...)
!r" : 1500
(...)
__ 8.16 .. ( - )
I-:,.; n ; i (-)
,n!` 131 ' (-)
258
<D.L. (" ) 1.2 (-)
Adjusted Effluent 20.5 (--) 20 (-)
1 N/A
8.14 (-) 7.9 (-) N/A { ` N/A
N.D.
1
, N.D.
Dilution W ater 20.1 (-) 20
(.,, )
N/A
8.01 '(--)
7.9 (-) N/A
N/A
N.D.
N.D.
4Efflucnt parameters measured upon rcipt of sample in lab and after adjustment on day of use. Dilution water (Wimex hw-Marincmix in ASTM Type I deionized water) values measured after preparation of diluent on day of use.
D-363
US00001544
ODI 'O C E A N A Sample I.D. M ixed Liquor IX 1.97 Sample 97-13669 Test ID: JR A 97I60
Coastal Bioanalysts Inc.
I. TEST RESULTS
% Effluent Cooeejitialioii
0
6.3
12.5
25.0
50 0
100
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acute Test Results
Mean (%) Organism Survival
M . bahia
C. variegatus
2 48-h
2 48-h
100 100
100 100
100 100
100 100
100 100 95 90 100 100 100 100
100 100 100 100 95 95 100 100
>100%
>100%
N/A N/A
N/A . N/A
<1.00
<1.00
Acute Test Information:
Test Type: ' End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
M . bah ia Test
48-h Static
Survival
*
12/2/97 1600
'
12/4/97 1610
Chesapeake Cultures, Inc.
12/2/97
20 g/kg
19.0-20.7C
No
N/A
C variegatus Test 48-h Static Survival 12/2/97 1600 12/4/97 1615 Aquatic Bio Systems, Inc. 11/30/97 20 g/kg 19.0-20.7C No N/A
1Aeration necessary when D.O. concentration <4.0 mg/1.
D-364
US00001545
Coastal ioanalysts Inc.
ODU/Oceana Acute Effluent Toxicity Tests
M ysid o p sis b a h ia & C yp rin o d o n va rieg a tu s
Sample I.D. Mixed Liquor Contract No. N00187-93-D-9748 s Delivery Order No. 0235 J.O. No. 1912290, Item No. 0002BJ, 0002BH
PWCNo. 1387 12/1/97 1330 Sample No. JRA97-13669
Submitted to: Ms. Merrill Anderson-Ashcraft . Navy Public Works Center
Code 930, Building Z-140 Norfolk, VA 23511
9
By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062
(804) 642-0168
D-365
US00001546
Print & Sign Names
Relinquished by________
Date
Received by
Date 1^-A -9 - 7
Relinquished by...r t a J n ----->JAW/^ ^-1 - Date Q
T)
Received by--- - f t> w
Date 1a - - 4 n
Time_______ Time________ Time ^
T im e
D-366
US00001547
Toxicity Test Sample Chain of Custody (Please complete all information)
Faciiity_ Q \ d D o m i n i o n ( . W v e r ^ t - k j
Address,.. ^ V A
^
REED Sj
County, NPDESf,
Pipe/Outfall/Location Instrcam Waste Cone
Sample'collected by (print&sign) v T b S O r \ / f ^ v y ^ . Affiliation fiQ t* E W . . l a U
Type of sample ^ (Grab):
Date, \2 -/ Q \ / ^*7 Time 1 1 * 0 0 &f\.
.(Composite): From Dace,
Time,
To Date
Time
Subsamples comprising composite:
Number___________ Frequency of collection_________ Volume________ _
'Temperature of sample in sample collection device O *Gs
Final temperature of effluent at sample collection point
'Is sample collection device chilled?^ e s _ Is sample packed on ice for shipment?
It is required that all samples remain at 0 - 4C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency (Do not freeze!)
Is the sample chlorinated?
dechIorinated?_jV3_ If so, how?_ V a . ____________
Permit with interim chlorine limit? -- If yes - limit fmg/L) _______________
Field pH P t f
Field Total Residual Chlorine___________
_____Comments/Sample description, Fp j^ A S t o c k .
-_______
Type of test(s) to be performed M\Hi\dL &Vr,'AP . C riA p rin vd C X i
(Specify organisms) _
Method of shipment OP IvOT
P n n t & Sign Names 1 . Relinquished bv ft. M g rtert- U n*# Date.
^ 0 0 CV*"*Time. {Q t 0 0 **V
Received by,
Date ( / I 'T '?
Time Q 9 f S -
2. Relinquished by. - / /
j QJLp >Date / (
pcejved bv
Date../gfr4
J 1 2 s-. . , t t i3. k t.FD //V-T"
Time / O ^ Time /<^ YO
FOR REED LAB USE ONLY
JRAjf
O Arrival Temperature_________
Color_________________ _____ Odor____
pH_________ DO (mg/L)_
Conductivity (umhos/cm)_
Salinity (ppt)____ TRC (mg/L). _____ Method_________
On ice? . Solids,
'K v
.
D-367
3cv 5/'.6/94
US00001548
COASTAL BlUANALVSTS INC. HrFLLENT/DILUTION VA1CKDAi A ^ronrr. cruu
INITIAL (ARRIVAL) EFFLUENT CHARACTERISTICS:
Sample Date I M / / / 4 2 1 1 i - 1
1
Temp. (C) I 3 . 1
1
1'
1
1
1
Conduct. (uMHOS) l/V.-.c, 1 1 1 ! I 1
Salinity (g/kg) | /
I
I
I
!
I
I
pH n . i #
1
,1
1
Diss. Oxygen (mg/1) l / l L b 1 1 1 ! 1 1
Tot. Chlorine (mg/1) 1 L D L 1 1 1 1 1
NH3-N (mg/1) Hard (mg/1 CaC03)
i 1 !1 1 11!
o In
Alkln (mg/1 CaC03) i 3 0 f 1 1 1 1 1
CoTor/Appearance1 i c y
1
1
1
1
!
1
. Initials
IC-cV l
l
1
1
1
1
1 C-Ciear O-Opaque Y-Yellow B-Brown BI-Black G-Green T-Turbid SI -Solids (S-SIight M-Moderate V-Very Much)
EFFLUENT ID:-for r\ 54f>tR____________ TEST ID: ^ [ 1 l9 1 -f <5-L D-368
US00001549
EPA P R O B IT A N A L Y S IS PROGRAM USED FOR CALCULATING L C /E C VALUES
V e r sio n 1 .5
JR A 97159 FE E D STOCK
C one.
6 .2 5 0 0 1 2 .5 0 0 0 2 5 .0 0 0 0 5 0 .0 0 0 0 1 0 0 .0 0 0 0
Num ber E xposed
20 20 20 20 20
N um ber R esp.
0 0 5 19 20
O bserved P ro p o rtio n R esp on d in g
0 .0 0 0 0 0 .0 0 0 0 0 .2 5 0 0 0 .9 5 0 0 1 .0 0 0 0
P ro p o rtio n R esp on d in g A d justed fo r
C o n tro ls
0 .0 0 0 0 0 .0 0 0 0 0 .2 5 0 0 0 .9 5 0 0 1 .0000
Chi Chi -
Square fo r Square fo r
(ta b u la r
H etero g en eity H etero g en eity v a lu e a t 0 .0 5
(c a lc u la ted ) le v e l)
= 0 .0 2 8 = 7 .8 1 5
J R A 9 7 1 5 9 FEED STOCK
E s t i m a t e d LC/EC V a l u e s an d C o n f id e n c e L im it s
P o in t
LC/EC 1 . 0 0 LC/EC 5 0 . 0 0
E xposure 'C one.
1 5 .4 3 5 3 0 .6 2 9
95% C o n f i d e n c e L i m i t s
L ow er
U pper
8 .7 7 8 2 6 .0 8 3
19 .6 2 3 3 6 .2 5 3
i
D-369
US00001550
L*c),\.s r . u HlO.XN.U.YSTS. INC. NPDfcS STATIC ACirrE/SALT'WATfcR
(Korm ACV4X)
1 EMfu.ni
10
0t
*rl Lrv*
Control
C-l 10 C-? >0
M 10 S.25 1*2 i O
20 10 12.5 2-2 0
30 i (< 2S 3-2 ' 0
40 o
550 4-2
SO 0 100 5-2 O
Obitver
Crff
ma: ( S D
Oay 2
$ L>v
lo
(o fo [O ( L>
f0
9 10 1 0 0
0 P& .
1^
Final 1
SitfVrvd
i o
l Oo
|O fc
~7 5
5.
6
^ucigs: Cypnnodon vaneqatus______
HiichU^tw'lurs;____ \ i j S o / ' n
AfclTMt>on r*l*r HWM ASW ^ I O tta r^
Accfm w tiun Tvirp. (rai^jc)
/Q
ScKjrc:
Sd.ft.rr "2^0 -Z--Q--7-
Fw doj dunny last: Nct fvd C?1 Artrr*a
H Oth*r
Chantar su*: 300 irt
500 nT
lOOOrr. 1 2 Ollw.
Ttst luna: 230 rrt
2S0rt Q
500 n. J 2 O tw .
Intu no. oroinsm pr radcta:
fO
Na. npical
iri
ftotopanod 161:90 8
hr
RantbnmiMn tanvfeti no.[_ J 3
setup. o .
f 2 _ / 2 . / ^ Tina Wur A<fcfcd_ i s r r
Tina AnfTtth Aochd
__St Up BvC -.^ 3
NSIHUMENIATIONUSED:
Slm TUiTT*lf Nufflbtr
i_____
T 3 0.0. Mlr > t (SJ601W SJ
3 CP DignmM !UtiK(095C0563)
Sanwius RI60P Balan (.0020083) G TS SCT 33 Matar (H30I6792)
Q TS 0.0. HUI 2 (3B1AQ26323)
U C m n 9 240 I * M* " ' (96268)
SP
Katar (8S23S0)
3 S p v m A7C Rafncuralar (A366)
0*y0
Cy I
C 3>-/
T c ,y
I
1 .Q /;>
*0 ?
E2
M
P3
. 3 7 2 C-.
4 7?
5 7 >.i>
c ?.3
1
PH 2 - ) - >
3 Z .o J -
'^ - 7 0
4 c - C 6
5 T -Y rl T . f?
C \A
7 .^
1 ^D
0.0.
(mgR)
2 3 (j.
'7
4
5
SC A1 L
2 N3
4
S Raphcit* *
Infidt:
0 .^
7 L .1
e-3 *
7 -.Q -7 .-.
0 c. r> (:
0o
U A
0 ft
ao AO
1
WS
Q f. 2< .
)
P/3
0*y 2
P o '3 P .y ^C -v
v -- > .S 7 7?7 7 .9 ? S 7*0 ? .fP . -- 7S 77 7 fc 7l 7. --
PC
c7o -- j? P6
NOTES: -fr f > 0 , V . A .
W\iA O ------------------------------------
Qudity A iu n n u :
-
As QuWtty Asswane* oHcf tor ths tst I ccmty th*t wyrutc*nl
tNwdions ffwn aeccpud pretocul uccwrrt ufwylOwtmr mspectiunf |wJss OlhKWiS* noi*d ta lo *) And XMtha (&U ccurtly ivkCI Xhe
tMt m wa* prfornad fcy C8l.
Con-nanc
~T
\ i
(
\
TEST i0 : ^ 4 ^ 5 3 1 1 5 D-370
US00001551
t r im m e d s p e a r m a n - k a r b e r METHOD. VERSION 1 . 5
DATE:
1 2 /2 /9 7
TOXICANT ; FEED STOCK
SP E C IE S: M BAHIA
T E ST NUMBER: JR A 97159
DURATION:
RAW D A T A : ----------------------
:
C o n cen tra tio n (%)
.0 0 6 .2 5 1 2 .5 0 2 5 .0 0 5 0 .0 0 1 0 0 .0 0
SPEARMAN-KARBER TRIM:
o o
N um ber E xp osed
20 20 20 20 20 20
M o r ta liti
0 0 0 0 17 20
o Q
S P E A R M A N - K A R B E R E S T I M A T E S I:
L C 50:
95% LOWER C O N F ID E N C E :
95% UPPER CO NFIDENCE:
3 9 .2 3 3 5 .1 2 43.82
48 H
D-371
US00001552
CO AST.U . UIOANALYSTS. INC. N PD ES STA TIC ACLfTE/SALTWATER
(Form .A.MB-JX)
\ Effluii
Day 1 1.0 . 9 live
Day 2 9 In *
6.25 12.5 ?s 50 TOO
C-l l o C-2 <<s
1-1 > 0 1-2 _____i l l _________ 2-1
10 i <j (c ,
(0 1
2-2 / O 3-1 1 0
(o ( c>
3-2 f
34-1
(0 o
4-2 4 5-1 & 5-2 a
Obst rver. Tin:
C >H \*Z \o
___________ o
______C __________ . . . _ & __________
1 (tfM )
final % Survival
|0 O
lO o to o
IC O
le>
0
DayO ______Day 1_________ Day 2
C 3 V ).I
> c .5
_ ^ 0 j_
T
i?> ( 7
OM
E
2
o ? p .y
-K >
M
TO v
p 3a
2 ft 7
3C v
4
j (7
Z H j.i
s
,v o .y
--
c * .< 3 7
7 -1 /
-, e 7
1
"M V
7 -?2 _
2 S .3 0
W
?97
pH 3 t i . c l - 7 - 3 J C c i
4 7- I * S - O ... 3
7 7-35 -
^ .c -1
--
c 73
3 . S'
73
1 > .5 -
7-7-
77
D.O. l/ryyl)
2 ? .3 3 b -y
> -V >3
7C , 73
4 li> .7
5 (x -3 *
sC
A 1 ) i) l
2 - 17
N3
D
4
5 Radicate 9
1
Initials:
C /
motfs * rro
brKJjA s~> ,
7 -3
77
73
--
7>r>
3>C
J o - ....-
3>o
ft 7 r-.
7<3 <5>
7 r; 7 ft
s>o
---
7 77
y n>
f
i<j>y s ~, r /t
----------- -------
X. Quality Assurance: As QuJity Assurance officer for this test l certify that no s u fic a Aviations from accepted protocol occurred diong routirw inspections (unUn otherwise noted below) and that the data accurately fleet the tasi as >( was performed by CBl.
Ai
tL jJi 1 Pyu
TEST I D .:_ J jjls _ 5 L 2 liii
D-372
US00001553
O{j
CUMULATIVE DATA SUMMARY NPDES Permit #: N/A Oceana - ODU FeedS.
DavteofrSr;r;1 Inveib&raf
2/12/97 <6.25 2/28/97 Invalid 3/12/97 17.7 3/20/97 19.5 3/26/97 33 9/30/97 8.1 11/3/97 12.5 11/18/97 23.3 11/25/97 9.7 12/2/97 39.2
i
Vertebiria-4t-ew'xas.j-w-* LC50 % ~ 31 35
52;
35 34 3.5 19.7 36.6 13.8 30.6
Invertebrate ;Vv.:?> Vertebr:-ari.t,e^.a4i-4.Si:f^5rf^s^: NOEQ % - *'
Supplement
D-373
US00001554
O D I' ( ICE.VN'A Sam ple I D Feed Slock 12 1 9 7 Sample 97-13670 Test ID: JR A 97I59
III. OUTFALL DATA SUMMARY
Coastal Bioanalysts Inc.
Test Pate
9/30/97 11/3/97 11/25/97 12/2/97
Table 4. Cumulative Data Summary - Acute & Chronic Tests (Tests conducted on this outfall by CBI)
Invertebrate
Acute Tests
Chronic Tests
LC50 T.I1.
NOF.C T.U.
8.1% 12.5% 9.7% 39.2%
12.35 8.G0 10.31 2.55
N/A N/A N/A N/A N/A N/A N/A N/A
Vertebrate
Acute Tests
Chronic Tests
LC50 T.U.
NOEC T.U.
3.5% 19.7% 13.8% 30.6%
28.57 5.08 7.25 3.27
N/A N/A N/A .N/A N/A N/A N/A N/A
` Results submitted with this report
IV. QUALITY ASSURANCE
M. bah ia - Acute
Reference Test Dates:
Reference Toxicant:
Test Concentrations:
Control Survival:
LC50 Reference test (95% C.L.):
Control Chart LC50 (95% C.L.):
C. variegatu s - Acute Reference Test Dates: . Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C L ) Control Chart LC50 (95% C.L.):
Chesapeake Cultures, Inc. (same batch as test) 12/2/97-12/4/97 KC1 (Sigma "Ultra" Lot#76H10391) 343, 490, 700, 1000 mg/1 100%
606.9 mg/1 (578.6-636.7) 608.2 mg/1 (489.3-727.0)
Aquatic Bio Systems, Inc. (same batch as test)
12/2/97-12/4/97
.
KC1 (Sigma "Ultra" Lot#76Hl 0391)
432, 720, 1200, 2000 mg/1
100%
1169.7 mg/1 (1044.1-1310.5)
1074.9 mg/1 (890.8-1259.0)
.
D-374
US00001555
O D I' O CEANA Sample I D. F eedstock 12.1 97 Sample 97-13670 Test ID: JR A 97I59
II. WATER QUALITY
Coastal Bioanalysts Inc.
Parameter
Temperature (C)
DO.
(mg/1)
pH
Salinity (g/kg)
,
Control
20.3 (0.2)
7.8 (0.1)
8.01 (0.21)
20 (0)
Table 3. Water Quality Summary
M . bahia Test
Mean Value (Standard Deviation)
6 3%
12 5%
25%
50%
0 A
(0.2)
20.5 (0-1)
20.5 (0.2)
20.4 ' (0.2)
7.6 (0.1)
7.4 (0.2)
7.2 (0.4)
7.3 (04)
8.07 (0.20)
8.03 (0.18)
8.00 (0.07)
8.00 (0.21)
20 20 20 20 (0) (0) (0) (0)
100%
20.5 (0.3)
6.8 (0.6)
7.72 (0.42)
20 (0)
Parameter
Temperature (C)
DO. (mg/1)
pH
Salinity (gAg)
Control
20.4 (0.3)
7.8 (0.1)
8.05 (0-19)
20 (0)
C. variegatus Test
Mean Value (Standard Deviation)
6 3%
12-5%
25%
50%
20.4 (0.2)
20.5 (01)
20.4 (0.2)
20.5 (0.2)
7.5 (0.3)
7.4 (0.2)
*7.3 (0.4)
7.3 (0.4)
8.09 (0.18)
8.07 (0.13)
7.99 (0.09)
7.96 (0.18)
20 20 20 20 (0) (0) (0) (0)
100%
20.6 (0.4)
6.8 (0.6)
7.65 (0-32)
20 (0)
D-375
US00001556
O D t o a - A N A Sam ple I.D. F e e d s to c k 12.1 9 7 Sam ple 9 7 -13670 T c t , d .
Sample Collection Date/Time
12/1/97 1100
. Sample Use Dates/Time<!
12/2/97 1530/15403
Coastal Bioanaivsts Inc.
Aeration2 Rate/Time
n/a
Pretreatment/ Adjustment
HWM Salts
i' s z z z s r i ' s r s" p"
" " ,o ioo%
-- warming.
Parameter
Temperature
(C)
Salinity (g/kg)
Conductivity (uMHOS/cm)
pH
Dissolved Oxygen (mg/1)
Total Hardness (mg/1 as CaC03)
Alkalinity (mg/I as CaC03)
Total Chlorine (mg/1)
n h 3-n (mg/1)
Table 2. Efiiuent and Dilution Water 4 Chemical & Physical Characteristics (Mean Value (Standard Deviation))
Initial Effluent
3.2
.<--)
1
(-)
1000
(-)
7.06
(-)
10.6
(-)
150
(-)
361
(-)
<D.L.
(-)
33.0
(-)
Adjusted Effluent 20.3 (...) 20
(-)
N/A
7.42
(-)
7.9
(-) N/A
N/A
N.D.
: N.D.
Dilution Water 20.3
(-)
20
(-)
N/A
8.25
(--)
7.9
(-)
N/A
N/A
N.D.
N.D.
h
^
n
n
Z
?
^mTASsTM^
TZ^ Type 1
dTel0nnle2Ce7d
t f SampIe in bb and ^ adjustment
water) values measured alter preparation
n * * f nse. of diluent on
Dilution water day of use.
(Wimex
i
D-376
US00001557
ODl.* <)CU AN A Sam ple I.D. Feed Stock 12 1 9 7 Sam ple 97-13670 Test ID: JRA97159
Coastal Bioanalysts Inc.
I. TEST RESULTS
% Effluent Concentration
0
6.3
12.5
25.0
50.0
100
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acute Test Results
Mean (%) Organism Survival
M . bahia
C. variegatus
24-h 48-h
24-h
48-h
100 100
. 100 100
100 100
100 100
100 100
100 . 100
100 100 65 15 00
100 75 75 5
00
39.2% , 35.1-43.8
Spearman-Karber 2.55
30.6% 26.1-36.3
Probit 3.27
Acute Test Information:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
M . bahia Test
48-h Static
Survival
*
12/2/97 1530
12/4/97 1630
Chesapeake Cultures, Inc.
12/2/97
20 g/kg
19:0-20.7C
Yes
12/2 at 1645
C va rieg a tu s Test 48-h Static Survival 12/2/97 1540 12/4/97 1635 Aquatic Bio Systems, Inc. 11/30/97 20 g/kg 19.0-20.7C Yes 12/2 at 1645
1Aeraiion necessary when D.O. concentration <4.0 mg/1.
D-377
US00001558
I
D-378
I US00001559
ODU/Oceana Acute Effluent Toxicity Tests
M ysid o p sis b a h ia & C yprinodon va rieg a tu s
Sample I.D. Feed Stock Contract No. N00187-93-D-9748
Delivery Order No. 0235 J O. N6. 1912290, Item No. 0002BJ, 0002BH
PWCNo. 1388 12/1/97 1100 Sample No. JRA97-13670 '
Submitted to: Ms. Merrill Anderson-Ashcraft
Navy Public Works Center Code 930, Building Z-140
Norfolk, VA 23511
ff
By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062
(804)642-0168
D-379
US00001560
Print & Sign Names
Relinquished by_______ '_________ ' Date____________ Time_______
Received bv
-- ' Date U J=^ '7 Time
Relinquished bv
^ _ Date 1A *7? . Time ____
Received bv ( i X v ^SLQo.-ocf) ____ Date \ 3 -~ei - <?'7 Time >
D-380
US00001561
Toxicity Test Sample Chain of Custody (Please complete all information)
Facil ity_ Q \ d s D o t m n i o n (/W iV 'ftrQ i- k i Address_
County_____ ~ _________ . NPDESir______________________
Pipe/Outfall/Location Instrcam Waste Cone _____________________
Sample collected by (printdesign) v l f o S O n Affiliation fiQ H & V . E r a . L n U
Type of sample ^ (Grab):
Date l Z / i P t / ^ 7 Time ^J'~SJ5 P f t\
^~
_____ (Composite): From Date________ Time_______ J
To Date
Time
Subsamples comprising composite: *Ya .
:
Number___________ Frequency of collection_________ Volume,
'Temperature of sample in sample collection device O .
Final temperature of effluent at sample collection point. `f - .O X L,
'Is sample collection device chilled? tj&S Is sample packed on ice for shipment?
*It is required that all samples remain at 0 - 4C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency (Do not freeze!)
Is the sample chlorinated?
dechlorinated? NO If so, how?_ ^ / a . ____________
Permit with interim chlorine limit? -- If yes - limit fmg/Ll _______________
Field pH_
Field Total Residual Chlorine,
Comments/Sample description. A l -
*/* k o \c r a J l r a f i o ^
Type of test(s) to be performed
. C c tjp r)'iW X X \
(Specify organisms) V a r f e ^ a f U - g ____________________
Method of shipment P liVflr*
P nnt & Sign Names
GO&/Y*. M -
Relinquished bv CV. MtyUgHeH-UnOjg Date |Z / g Z / l 7 Time -{0 * 0 $ * *
Received byv__ /aS L t-J ' Relinquished bv
Date Time <^? ./V/..CC f 3 3 ? Date J l 7 Time ( 0 <f - 0
Received bv
_ Date
7 Time
FOR REED LAB USE ONLY
JRA# ^ 7 ~ i ^ ( o 7 j
Arrival Temperature_________
Color__________________ _____ Odor____________
pH......
DO (mg/L)_
Conductivity (umhos/cm)_
Salinity (ppt)____ TRC (mg/L). _____ Method________
On ice? . Solids.
D-381
3c 5/16/9
US00001562
IN IT U L (ARRA'AL) EFFLUENT CHARACTERJSTICS:
Sample Date Temp. (C)
l^///^'/l
11
1 1
Conduct. (uMHOS) l/OOCs 1
1
Salinity (g/kg) | |
|
|
1 1
I
11 11 11
II
1 1 1
I
pH U . O 1
1
1
1
1
Diss. Oxygen (mg/1) i a . a i
I
1
1
1
1
Tot. Chlorine (mg/1) l L p c 1
1
1
11
1
NH3-N (mg/1) 1 ( . 1
1
11
Hard (mg/1 CaC03) I I V o 1 1 1
11
1
Alkln (mg/1 CaC03) | ) 5~ ) l i l i l
Color/Appearance1 K f
1
1
111
Initials
10-6
1> 1
1
1 1
1
1 C-Clear O-Opaque Y-Yellow B-Brown Bl-Black G-Grecn T-Turbid SI -Solids (S-Slighi M-Modcrate V-Very Much)
TTFFI LENT DJUSTMENT/POST-ADJUSTMENT MEASUREMENTS:
Date I I 1 1
Temp. (C) | | | 1 1
Salinity (g/kg) | | | 1 1
D O. (mg/1) | I I 1
Aeration Rate j | | 1 1
Aeration Time (min) | | | 1 1
Adjust. D O. (mg/1) | | | 1 1
pH | | 1 1 1
Tot Chlorine (mg/1) | | |
1
Initials
|
|
|
1
1
DILUTION WATER CHARACTERISTICS:
Date | | |
Temp. (C) | Conduct. (uMHOS) [
| |
| |
Salinity (g/kg) | | |
pH | | I
Diss. Oxygen (mg/I) |
|
Hard (mg/1 CaC03) | | |
Alkln (mg/1 CaC03) | | |
Initials
|
|
DMW:
1 1 1 1
1 1
1
1 1 1 1 1
ACUTE
1 1 ll/Q/O/T? 1 1 iiap.f
1 1 WQh
1 1 >.? 1 ! II1 i II 1 1 II 1 1 H-.ao 1 1 IL r y 1 1 Il i
ACUTE
1 1 ll'-a/a/i?. 1 1 Il Q 3
1 Il ^ Q 1 1 SO . 1 1 I U - ?" 1 1 IIL.L 1 1 Il
- 1 _____ IlA /& ___ 1 1 Il C-rt
EFFLUENT ID :_ _ \______________________ TEST ID: 3 ^ V i y <fy
D-382
U
US00001563
d.VSTAJ IIIO.VNALYSTS. INC NPDE5STATICACTTTE,SALTWATER
(K * m AOV-M)
* l Entwtu
' 9D#lifv1
Contre* C- ir. C2
U _ l_____ 6?S 1-2 _L-------
21 1u 12S 22 i C/
3-1 It, 25 32 1T
41 50 <2
SI 100 52
Qta<vc
1C
'___ Tf ...
7 SO
Turn: \S ^ b
90Lrr2 1' IU
ll> (0 10 (
(o
i0 10 iu q q
fb
htatvaw\rf ) oo l oo vOO ! oo
l oo
70
Smc* J /?T
H*rch i il 3 KT_ Acrlurutionwui*r HWMASW 0tir Aalmwtit (ran*) M - - to
L o i -----
tauiyta&t: NdIf*d J Ansra li OthAT
300 rH
SOC-rt
1000M 0
0 ti r __________
T*tt vohimi: 200 rri
01
5 0 0 -1 j a O ita :__________ n i
lnt>4 no. argjnw m fm* m t o a : ( Q - -
Pteio^nod 161.80
O ita:______ _ F t a ta m U M i t a * u
stiur. o*i (2. / 2.1<f~) im u m u m H-g I Q --------
II* \t> t f PTmtw A jw m lt A dOitf_
______________ S r t U o f t r O A
Iffel MUML N l A IIU N U A U
Jwo IhMm m vIM O TS 0 0 M*i*i * tS N tb O l^ 3 O ' I K m - m t* t M * r ( D ttb U O M J ) " J w i w m m m r i u i *- M to w iiN i|
| <<4 * I I t M.>* tlNW)lfi|
i T 9 0 . 0 . Umiw 9 2 | S t f l A 2 d 3 ? )
| C a n w ^ 3 4 0 {* M w { S M rfM )
n r IWO AAaia,IK.1JMJ)
. 1 . A II'H .,..,*.*,** tAtAAf
OatO Dr> D*r2
C 7/, <
T
T1
7- g .7.-c;
E2
y Z S ^70 s
u
P
3 Da L-
.2 fl. 7 J7C
4 5 /, ;c 7 PC')
5 .Zi.- 7 k 1
C 5 o l 7. t.'.v - r S ' i 1 S <jS' .y. /o 7 bP
2 Y tO
ia 7 9o
1*1 3 Z /P s- , T
4 W 2 > 7 3
S V.,)a
c ,
f/
7. 5
-V ?< T 7 S'
Zi 7 ^
0.0. 2 7- f (mfl) 3
7V 7 7 93
7 f /7--g---- 7-7
s
5 J t - 7. C c .3* .*C` #
n7 7e - :
A 1 O / L2
f '..?C -?c ' ,70 --
N3
2 c _ ^ o __ :
tsr-M 4 5
.~lAy(r' --
-to
2.0
tlv
fWffcc# 1
,709-
__J----- ;
Iniuls: > n __ e a
NOTES
TEST I D. : TT( l^ * P M z L
D-383
US00001564
COA.ST.U. UlUAN.-vLYSTS. INC. NPDES STAT1C ACUTE/SALTWATER
(Forni A M I*)
lE ltlu v it Control
6.25 12.5 25
1.0.
0T 1 f Liv*
1V
C-l
C-2 I O
1-1 < b 1-2 i
2-1 i -,
2-2 t N 3-1 1 3-2 t
Dr2 $ Inra
Final \ SovivaJ
0 _ L __________
(0
-- U __________ (D io
l O \o o [o o
lo
| 60 (O
4-1 ifc 50 4-2 . . K )
5-1 ! O 100 5-2 1 S
< 0 _________ (O (0 (>
U >o
Ottdrvor
60
Timo:
1 5 3 j5 T
_______
-: } --. -
I
1
S n ita :
IS T C -^ .
- --.
Match OatWYiiro:!
Accliuwiion Ttrrp. (ranga): .
1^ .o
lo .1
f OtU r .
.
Fea&r^ dtfing tati: Noi fod L J A/tamii I V fd Othor
C h i . : 3 0 0 itJ ( 3
500/rt
1000/rt
O ttT __________ tri
Im i wlurm:
^P
250 rrt
500 ai
Othor__________ rrf
Indiai no. or^aniwr* por radicala: / O
No. raflcoiM:_
P hotopanod 1 6 i:8 0 3
0 t f r ___________ R a n r m a t u n t o m 0 a u n o
g T u p 'P M ^ T T 2 - \ t- (
T im i W u r A A h d , . j L g , , L Q ____________
T im i A ra rm i* A t t t d _ I V ^ L>____________ S r t U p B y C r T ^ ? P 'J ^ Q
INSTRUMENTATION USTO:
Siim Thintnim w Nurtw: f t I O T 3 0.0. M*i*r f 1 1 S i 8014475)
[ CP Digramma pH Motor (D950QS63) I Svtonut R160P Bilanci (40030093) YS SCT 33 I to u r (H8016792)
Q j Y 3 D.D. Molar # 2 (St1A02S329)
r i i Corning 240f H Motor (36268)
SP P o rti MiUf (852360) Sparivi ATC Rafnctomatar (A366)
Othor
0#r 0
0-yl
D*y 1
C
l e (,
J-c s
-T E M
l 2 3
* P -'J
J .0 c
; * ? .u
Po L
4 <=? d w s -3, &
?n H
20 - H
P C fr jp e i
C r \X %' o 2
3 / Ln-- ? . ` H
- (O 7 .?A
2 R i r-i
Cu 7V ?
pH 3 ./3
t . Il
S!' C O
4 R .lf'
2-G
? 5
5
c 7 - .1 l 11
T. r 7^
~i "7 ?
D-0. [mQfl)
2 -----J----.--ff ------- 7 . 7
3 7-.1
7- 7
'7 8 -7 5
7 - .f
7t
7?
5 ^ 7 .._
sc
Al L
2
N3
(IFVJ)
4 5
Radicati I
.O r,
o-
-O h il b 0 ( '.
,,,, '
Z L`
2c '
5 -0
i' O
cP O
Ih 1
cT
Do
Irabali:
SO . e s t -
NOTES:
Quohry Ai u m c i :
As QualrryAs s u m o oHear for tisi tosi I comly th *
dfviMicfU Iran accopiod protocol a c a m d AmnQ rooirra iftsptcnons
[uNots otNtfwua iwtod
and thot tho dai oceuntaly roHoct iho
taot m il voto parforrmd C8I.
Corrmanu
/7 / ,7 7 > v ^ # w ( V 7 " y7 i i l s k q
Do* '
*\/ .
TEST I . D . : _ ^ jl A 3 2 - L k i
D-384
US00001565
CUMULATIVE DATA SUMMARY NPDES Permit #: N/A Oceana - ODU A-l
iDte of; * Invttrais^fe^,.,,* " Vertebrate'
I 2/12/97 >100
j 2 /2 8 /9 7 Invalid
3/12/97 >100 3/20/97 > I0 U 3/26/97 >100 9/30/97 > 100 11/3/97 >1UU 11/18/97 > 100 11/26/97 > 1 0 0 12/2/97 > 1 0 0
>100 >100 >100 >100 >100 >100 >100 >100 >100 >100
.
Invertebrate.^ -1:> r -' TJ- 5-
Yertebratec-*-'`-
NOEG'% ~ ^ y ;-:: ' -
`
.
*
Supplement
D-385
US00001566
O D l ' O C E .V N A Sam ple (.D . A l
I Z 1 97 Sample 97-13671 Tesi ID: JRA9716I
UI. OUTFALL DATA SUMMARY
Coastal Bioanalysts Inc.
Test Date
9/30/97 11/3/97 11/25/97 12/2/97*
Table 4. Cumulative Data Summary - Acute & Chronic Tests (Tests conducted on this outfall by CBI)
Invertebrate
Acute Tests
Chronic Tests
LS& T.U.
NOEC T.U.
>100% >100% >100% >100%
<1.00 <1.0.0 <1.00 <1.00
N/A N/A N/A N/A N/A N/A N/A N/A
Vertebrate
Acute Tests
Chronic Tests
LC50 T.U.
ttPSC T.U.
>100% >100% >100% >100%
<1.00 <1.00 <1.00 <1.00
N/A N/A N/A N/A N/A N/A N/A N/A
Results submirted-with this report
IV. QUALITY ASSURANCE
M. bahia - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50,(95% C.L.):
C variegatu s - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
Chesapeake Cultures, Inc. (same batch as test) 12/2/97-12/4/97 KC1 (Sigma "Ultra" Lot#76H10391) 343, 490, 700, 1000 mg/1 100% 606.9 mg/I (578.6-636.7) 608.2 mg/I (489.3-727.0)
Aquatic Bio Systems, Inc. (same batch as test) 12/2/97-12/4/97 KC1 (Sigma "Ultra" Lot#76H10391) 432, 720, 1200, 2000 mg/1 100% 1169.7 mg/1 (1044.1-1310.5) 1074.9 mg/1 (890.8-1259.0)
!
D-386
US00001567
O D I'.OCEANA Sample I D. Al 1 2 .1 9 7 Sample 97-13671 Test ID: JR A 97I6I
EL WATER QUALITY
Coastal Bioanalysts Inc.
Parameter
Temperature
(C)
' D O. (mg/1)
pH
Salinity
(gAg)
Control
20.5 (0.2)
; 7.8 (0.1)
7.96 (0.12)
20 (0)
Table.3. Water Quality Summary
M. baha Test
Mean Value (Standard Deviation)
63% 12J% 25%
50%
50.5 (0.2)
7.8 (0.1)
7.95 (0.13)
20 (0)
20.5 (0.1)
7.8 (0.1)
8.00 (0.11)
20 (0)
20.6 (0.1)
7.8 (0.1)
8.08 (0.07)
20 (0)
20.5 (0.1)
7.7 (0.2)
8.17 (0.03)
20 ' (0)
100%
20.6 (0.2)
7.8 (0.1)
8.25 (0.05)
20 (0)
Parameter
Temperature
(C) DO.
(mg/1)
pH
Salinity
(g/kg)
Control
: 20.5 (0.3)
7.9 (0.1)
8.00 (0.14)
20 (0)
C. variegatus Test
Mean Value (Standard Deviation)
6 3%
12.5%
25%
50%
20.5 20.6 20.6 20.7 (0.3) (0.2) (0.1) (0.1)
7.9 7.9 7.8 7.8 (0.1) (0.1) fa. I) (0.1)
8.00 . 8.04
(0.16)
(0.12)
8.10 (0.05)
8.20 (0.03)
20 20 20 20 (0) (P) (0) (0)
100%
20.7 (0.1)
7.8 (0.1)
8.25 (0.05)
20 (0)
D-387
US00001568
O D f'O C IiA N A Sample I.D. Al 11 1.97 Sample 97-13671 Test ID: JRA97161
Coastal Bioanalysts Inc.
Sample Collection Pate/Timg
12/1/97 1925
Sample Use Pates/Times
12/2/97 1630/16303
Aeration2 Rate/Time
n/a
Pretreatment/ Adjustment
HWM Salts
2Aeration often necessary to reduce sample oxygen concentration to 100% saturation after wanning. 3AI. hahia'C. variegatus test time.
Table 2. Effluent and Dilution Water 4 Chemical & Physical Characteristics (Mean Value (Standard Deviation))
..caramster ,n 'c. i i; t Temperature
(C)
Salinity (g/kg)
Conductivity (uMHO'S/cm)
pH
Dissolved Oxygen (mg/1)
Total Hardness (mg/1 as ta C 0 3)
Alkalinity (mg/1 as CaC03)
Total Chlorine . (mg/1)
n h 3-n (mg/1)
Initial Effluent
2.7 (-)
1 (-)
' 1000 (-)
8.42 (-)
11.2 (-)
140 (-)
252 (-)
<D.L. , (" )
1.1 (-)
Adjusted Effluent 20.8 (-) 20 (-) N/A
8.20 (~ ) 7.9 (-) N/A
N/A
N.D.
N.D.
Dilution Water 20.3 (-) 20 (-- ) N/A
8.07 (-) 7.9 (-) N/A
N/A
N.D.
N.D.
4Efllucnt parameters measured upon receipt of sample in lab and after adjustment on day o f use. Dilution water (Wimex lnv-Marinemix in ASTM Type I deionized water) values measured after preparation of diluent on day of use.
D-388
US00001569
Onr-OCKANW sam ple .D. Mother Liquor 11 24.97 Sample 97-13440 Test ID: JRA97I52
III. OUTFALL DATA SUMMARY
Coastal Bioanalysts Inc.
Test Date
9/30/97 11/3/97 11/25/97*
Table 4. Cumulative Data Summary - Acute & Chronic Tests (Tests conducted on this outfall by CBI)
Invertebrate
Acute Tests
Chronic Tests
LC50 T.U.
NOE.C U L
>100% <1.00 >100% <1.00 >100% <1.00
N/A N/A N/A N/A N/A N/A
Vertebrate
Acute Tests
Chronic Tests
LC50 T.U.
N.Q.EC. T.U.
>100% <1.00 >100% <1.00 >100% <1.00
N/A N/A N/A N/A N/A N/A
` Results submitted with this report
IV. QUALITY ASSURANCE
M. bah ia - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
C. variegatu s - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
Chesapeake Cultures, Inc. (same batch as test) 11/25/97-11/27/97 KC1 (Sigma "Ultra" Lot#76H10391) 343, 490, 700, 1000 mg/1
100%
580.2 mg/1 (490.0-700.0) 609.2 mg/1 (488.6-729.9)
Aquatic Bio Systems, Inc. (same batch as test) 11/25/97-11/27/97 KC1 (Sigma "Ultra" Lot#76H10391) 432, 720, 1200, 2000 mg/1 100% 1140.2 mg/1 (1019.5-1275.3) 1072.6 mg/1 (887.0-1258.2)
D-389
US00001570
CUMULATIVE DATA SUMMARY NPDES Permit #: N/A Oceana - ODU Mix L. R.R.
D ateof Iavertebrte^lT- Xest-'f.." L C 5 0 * f r ^ \ - - '
2/12/97 >100 2/28/97 Invalid 3/12/97 >100
3/20/97 >100 3/26/97 >100 9/30/97 >100 11/3/97 >100 11/18/97 >100 11/25/97 >100
Vertebrteglj
>.'mVma*"
.Scs---
LC50,%--''K
>100
>iop
>100
>100
>100
>100
>100
>100
>100
Invertebrate v - v Vertebrate ' . * NOEC % > T" ' NOEC % -
SuDDlement D-390
US00001571
COASTAL BIOANALYSTS. INC. NPDES STATIC ACUTE/SALTWATER
(Form AMB48)
%Effluent 1.0.
Control C- C-2
t-t 6.25 1-2
M T2.5. 7-2
3-1 25 3-2
4-1 50 -7
5-1 100 5-2
Obserrer Time:
D#Lenyrfe
1 0
ID
10
0
10
f0
1b
1b 1o
1D fb
P<3> 15 )0
OtLnnr2e io iO 16
1 1c
7 %
'0
?
fc.
<`
50
^50
Find\
Survrvd
\1ftp
/
Scem i: Mvsidopsis bahia
Source:
-^81- 0-g-
Hatch OetafTfre:
h I z - ih i
Acclinretion water. HWM ASW O ' Other
SeTmrfir D O
Aeritration Ter^J. (range):
to . 3 0 9
Feeing prior to te s t Artemia r ? *
Id Other
fed CFeedng derng te s t Not
1 Artansa / A M
Other
0Chanter sire: 300 ^
500 ni
1000 irf
Other
T ehem ; J 0,H 0
250 nJ
500 n9 Other
iN rrf
Initial ne. omantsne per reificete:
I T ) _____ No. recficatar 3 -
..
0f f c topenod 161:80
3Othar___________ f ta n d o r n r a tio A ta r s i a t a no.
^
nSET UP: Data,
f i t o n TtrmWatar Added
Tam Armai Added_ K t > < "
SetUgBy f P f f
INSTRUMENTATION USED:
Stun TT.iT tXT.TFf Nwnfew, f t I
YS 0.0. Meter 1 [SNE8014475)
3.CRO ^ M tn .1 * Meter (09500563) I] SerlonwRISOPBeleree (40020093) rsi SCI 33 M.ter (H8016792)
0 rsi D.O. Meter # 2 (SN91A026329)
Cfi*"9 240 (*( Meter (3 6 2 6 8 )
SR PortpH2 M .ter (852360)
0 Spettiti ATC Befrectoimter (A366)
Other______________ ________________________________________________
C
Tt E2 M P3
4
s
c
I
2 (
3 4
s
c
1
0.0. 2
3
4
5
sC AI l 12 N3
4
5 Replicata t
Initials:
Per 0_________ P e t )
T on
pej
< ?o -7 <7t> K,
2 0 -1 2 0 .2
2 ^1
Z io V
PO - I Po O
& 76
70 201
1 2 'i
0%
<Z 2 0 9 Ol 2 .1 ? ? <7 7 C7 7?
9 -C 9
9 10
3 21
.fll 7? 7 .9
71 7 -9 ~?7
'7 * 7?
7 -7
no
T ie
2
0*
70 P -o
n 11 19
9
1%
a1
On 2 2o. 1
20-3
5 0' 3 20. i 2 l- V 2-' - Y 1 .7 g 1211
r ,o g. C 7 6 -9 J f i * c O
7- 7 76 7 -7 7. S' 7. 7
2 .U L C
a
a '9
e & f & A n ______
NOTES
T>0 1 l -I >00 i.-^ (&0 C- ^O ^Q .
Quality Assvanea:
As Qudify A niranca otficar for this tat! t certify that no significant deviations from aocaptad protocol occurred during iwtma inspections (unless olhermse noted balm*} and that the cfcta accuetely reflect the test as it was perfonred by CSl.
C om tfnts
_
D-391
TEST I D :
iS X
US00001572
COASTAL BIO ANALYSTS. INC. NPOES STATIC ACCTE/SALTWATER
(Form ACV48)
Oo
\ Effluent
Oay 1 I.D. 9 live
Control 6.25
C-l / : C-2 _ i ________
-1 l o
1-2 1 0
.2-1 1 0 12.5 2-2 1 0 __
3-1 1 0 25 3-2 1 0
4-1 I 50 4-2 ( 0
5-t 100 5-2
Observer. Time:
(0
io pe>
I`H `3
Oay 2 9 live
10 fO >b >C
SO 0
:o lb
i<> ib
;0 1
5*? ...
( "W o
Final* Survival
Ve
\ oc
1OO \0 0 ,\0 0
gocci: Cyprinodon v a rie g a ti _________ Source:
/V ^_^
Hatch Oae/Time:___
t l / P 3 9 7 _________________ __
Accfiratiofl w ater HWMASW
Other
i s.n
to
Salinity: 2 - 0 r ? 0 <?
Eeedng prior to test: Artema . o x
/d Other
FmSng dunng test: Not fed 0
Artema
/d Other
Chamber sizr. ^OOH D
500 nJ 0
1000 ml OthK
T w v n ta .: 200.W
2SOn4 Q
500 H 0t!*r
lnt>al no. ornamsms per replicare:
ID
No. leuBcates:
ELPhotopeno< 161:60
Other
Randomization template
H ml
SET UP: Oata_ n / a i l r 7 Time Water Added,
Tim Animis Adtfcd / S 7 >
Set Up By. p n l P p j
INSTRUMENTATION USED:
AStem Thermometer Number l
Q Y3 0.0. Meter > t (SNE8014475) K CP Dgnense pH Meier (D950G563) Senonus R160P Balance (40020093) TS SCT 33 Meter (H8016792)
E3 Y3 0 .0 . Meter f 2 (SN91A02S329)
I I Coming 240 pH Meter (SN5268)
SP PottapH2 Meter (852360) E J Spartan ATC Refractometer (A366)
Other
Q*rO
C lo C
T 1 lo y
E 2 IsO.'i
M
P 3 Vo A 4 3 .
5 A
C % tA
1 %'?')
2 z .K
pH
3 &7A-
4 ,U i
5 %-Cr,
c 1 .9
1 2D.O. 1 1
{mfO 3 1 1
4 11
5 11
S C UO
A
l
! vo
I2
N 3 /*)
(Vg)
4
5
cate 9
tisis:
n
/V t?
Day 1
o< X 10 X -% 2 2-5 7^.9 lo. 1
1.9 1
g ..
i.o o
%JC>
71 7.9 7-? 7.7
11
1-1 O-o Oo lo
i9 t i
fe
Oay 2
o r.o
20 1
2- i
1*1.9
L. /
0 .0 .,
7. vj"
r . /
& c t r
Y ./^ 2 /r T .
sr,3/
1. ? 7 <f
7. 7
;. 7
7. e-
7.T 2C *2f
Zo
n
c/
SS
X
so
NOTES:
S po f-O. I 5~LwTj rfJUcgJ QbLurVlP_______
Q , 0 . l \ *i~ Sio-Pfl \ a lfr o %
Quality Assurance:
As Quality Asswanca officer for this t n t I certify that no slyaficant Aviations from accepted protocol occurred dtfing routine inspections
(ittless otherwise roted below) and that the cbta accurately
lh*
test as it was performed by C8I.
TEST I P.: A t - f i ^ 1 1 S D-392
US00001573
COASTAL BIOANALYSTS INC.
EFFLUENT/DILUTION WATER DATA (Fom EFDWi93>
INITIAL (ARRIVAL) EFFLUENT CHARACTERISTICS:
Sample Date \ u i ^ P n \
1_______ L _
1
I
I
Temp. (C) 1 i o 1 1 1 1 1 I
Conduct. (uMHOS) 1 Hob 1
1 .1
1I
I
Salinity (g/kg) 1 --
J
J
1
dH 19tSo I I 1 1 1 1
D iss. Oxygen (mg/1) f [ 0 . \ \ Tot. Chlorine (mg/1) I Z O L . I
____ 1_________!--------------!------------- L ---------- 1------------1 1 III.
NH3-N (mg/1) I < \
Hard (mg/1 CaC03) \ IvH
Alkln (mg/1 CaC03) 1 /s i
roln r/A oD earan ce1 1 C
1
\
1 1
1_________!--------------!------------- !-------- :-----L------------
1 1- 1 1 1 _ 1 1 1 1 1 .: 1 1 Ill-
----- - ^n't`a-------- -----------------^ r..
T-T.irhiit .SI -Solids (S-SIieht M-Moderate V-Very Much)
e r r i ITFNT APJUSTMENT/POST-ADJUSTMENT MEASUREMENTS:
E F F L U E N T ID :
L ,'^ (/p r
j 'T S T ID :. D -3 93
H a a < 77/57-
US00001574
Toxicity Test Sample Chain of Custody (Please complete all information)
Facility. f ) \ c L b i r u y J Q y ) L O u Y g f S r
%Address. M O r f b l K V A
Countv. NPDES#.
Pipe/Outfall/Location Instrcam Waste Cone
Sample collected by (print&sien) ' J . J
Type of sample ^/.G(GrarahbV):
DDaattee
.(Composite): From Date ~~
Affiliation O O ^ - 9a 1/^/ttSr U j i ?
TTiimmee *91 ^ 5
^
Time.
To Date___________ Time
Subsamples comprising composite:
Number___________ Frequency of collection. 'Temperature of sample in sample collection device O Q-*
Volume
Final temperature of effluent at sample collection point 3 . ^ 0 /
'Is sample collection device chilled?
Is sample packed on ice for shipment? LjG
`It is required that all samples remain at 0 - 4C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency (Do not freeze!)
Is the sample chlorinated? rtQ dechlorinated?
If so, how?
____________
Permit with interim chlorine limit?
If yes - limit (mg/L)____ ^ ______________
Field pH %.
Field Total Residual Chlorine__________
Comments/Sample description MdTf
f^ jcren c k R m ^ A e r )
r
Type of test(s) to be performed (Specify organisms)
Method of shipment
\V Hj/rPitGQ<Z f& j u S trtj O jA L
^
Print & Sign Names I. Relinquished bv Q *.b A vJ cf J tr~\Cn -
" / ^ T A 7 Time.
Recede d by.
Relinquished bv
J 6-^eC^-
D at
Date
e
J/!'i*/=g*^'Z'_~_tf_^_)
Time
Time
(
.
y
____ =
/ <3
#
/
Received bv P - Q icuzC r,
Date
Tm e / 9 (7>
FOR REED LAB USE ONLY
JRA#_
Arrival Temperature__________
Color.
_____ Odor________________
PH _
DO (mg/L)_______ Conductivity (pmhos/cm).
Salinity (ppt). _____ TRC (mg/L)______ Method_________
On ice?. Solids.
D-394
US00001575
Coastal Bioanalysts Inc.
ODU/Oceana Acute Effluent Toxicity Tests
Mysidopsis bahia & Cyprinodon variegatus
Sample I.D. Feed Stock Contract No. N00187-93-D-9748
Delivery Order No. 0235 J O. No* 1912290, Item No. 0002BJ, 0002BH
PWCNo. 1380 11/24/97 1035 Sample No. JRA97-1344I
Submitted to: Ms. Merrill Anderson-Ashcraft
Navy Public Works Center Code 930, Building Z-140
Norfolk, VA 23511
By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062
(804) 642-0168
t>-395
US00001576
ODI'OCK.VNA Sample I D. Feed 11 24.97 Sample 97-1.2441 Tesl ID: JRA9715 1
Coastal Bioanalysts Inc.
I. TEST RESULTS
% Effluent Concentration
0
6.3
12.5
25.0
500
100
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table I. Summary Acute Test Results
Mean (%) Organism Survival
M. baha
C. variegatus
24-h
4S
2 4 i 484i
8 OO
100 100
100 80
95 95
95 30
100 60
90 5 50 0
95 10 80 0
50 0
70 0
9.7% 7.5-12.0
Probit 10.31
13.8% 11.3-16.8
Probit 7.25
Acute Test Information:
*
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity. Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
M . bahia Test 48-h Static Survival 11/25/97 1500 11/27/97 1510 Chesapeake Cultures, Inc. 11/25/97 20 g/kg 18.9-20.8C Yes 11/26 at 1455
C variegatus Test 48-h Static Survival 11/25/97 1510 11/27/97 1520 CBI stock cultures 11/24/97 20 g/kg 18.9-20.8C Yes 11/26 atA 500
'D O . concentration decreased to slightly less than 4.0 mg/1 in 100% concentrations on day 2 of both tests, the drop was unanticipated and probably caused by a bacterial bloom (solutions turned milky color overnight). Low D.O. was not the primary cause of toxicity as indicated by low survival roncemrations with adequate D.O. concentration.
D-396
US00001577
O D I - O d iANA Sample LD. Feed 11,2*97 Sample 97-13441 Test ID: JR.A9715I
Coastal Bioanalysts Inc.
Sample Collection Dafp/Time
11/24/97 1035
Sample Use Dates/Times
11/25/97 1500/15103
Aeration2
Rate/Time
n/a
Pretreatment/
Adjustment
HWM Salts
2Aeration often necessary to reduce sample oxygen concentration to 100% saturation after wanning. 3.V/. bahkvC. variegates test time.
Table 2. Effluent and Dilution Water 4 Chemical & Physical Characteristics (Mean Value (Standard Deviation))
Parameter
Temperature
(C)
Salinity (g/kg)
Conductivity (uMHOS/cm)
pH
Dissolved Oxygen (mg/1)
Total Hardness (mg/1 as CaCO-)
Alkalinity (mg/1 as CaCOj)
Total Chlorine (mg/1)
n h 3-n (mg/1)
Initial Effluent
1.1
(--)
N/A
1638
(-)
6.98
(-)
10.8 (-- )
110 (-)
189 (-)
<D.L. (-)
37.0 (-)
Adjusted Effluent 20.2
(-)
20 (...)
N/A
7.31 (...) 8.0 (-) N/A
s
N/A
N.D.
N.D.
Dilution Water 20.5
(--)
20 (...) N/A
8.20 (...) 79 (-- ) N/A
N/A
N.D.
N.D.
4Efllucnt parameters measured upon receipt of sample in lab and after adjustment on day of use. Dilution water (Wimex htv-Mnrinemix in ASTM Type 1 deionized water) values measured after preparation of diluent on day of use.
D-397
US00001578
OD! ' OCHAN.\ Sample !.D. Feed I ! 24 07 Sample 97-13441 Tesi ID: JK.A97151
II. WATER QUALITY
Coastal Bioanalysts Inc.
Parameter Temperature
(C) DO. (mg/1) P
Salinity (g/kg)
Parameter Temperature
(C) DO. (mg/1) PH
Salinity (g/kg)
Table 3. Water Quality Summary
Control
20.3 (0.2)
7.S (0.1)
8.04 (0.15)
20 (0)
M. baha Test
Mean Value (Standard Deviation)
.
12J_%
25%
50%
20.3 (0.2)
204 (0.2)
20.4 (0.1)
20.4 (0.1)
7.5 (0.4)
7.90 (0.35)
20 (0)
7.4 (0.8)
7.82 (0.35)
20 (0)
7.1 (1.1)
7.54 (0.32)
20 (0)
6.5 (2.2)
7.81 (0.31)
20 (0)
100%
20.4 (0.2)
6.4 (2-3)
7.74 (0.35)
20 (0)
Control
20.4 (0.2)
7.9 (0.1)
8.00 (0.20) .
20 ()
C. va rieg a tu s Test
Mean Value (Standard Deviation)
6 3%
20.5 (0)
7.6 (0.4)
12J%
20.4 (0.1)
7.4 (0.7)
25%
20.5 (0.2)
j?
7.1 (1.4)
50%
20.5 (0.3)
6.5 (2.1)
7.88 (0.33)
20 (0)
7.85 (.36)
20 (0)
7.83 (0.31)
20 (0)
7.78 (0.42)
20 (0)
100%
20.5 (0.2)
6.5 (2-3)
7.70 (0.32)
20 (0)
.
D-398
US00001579
<)Ul '.OCKANA Santpic U > Feed 124.97 Sample 97-13441 Tesi ID: J R :\9 7 ]51
III. OUTFALL DATA SUMMARY
Coastal Bioanalysts Inc.
Test Date
9/30/97 11/3/97 11/25/97*
Table 4. Cumulative Data Summary - Acute & Chronic Tests (Tests conducted on this outfall by CB1)
Invertebrate
Acute Tests
Chronic Tests
LC50 UL
NOF.C T.IJ.
8.1% 12.35
12.5% 8.0b
9.7% 10.31
N/A N/A N/A N/A N/A N/A
Vertebrate
Acute Tests
Chronic Tests
LC5Q UL N.0E. UL
3.5% 19.7% 13.8%
28.57 5.08 7.25
N/A N/A N/A N/A N/A N/A
` Results submitted with this report
IV. QUALITY ASSURANCE
M. bah ia - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
C variegatus - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
Chesapeake Cultures, Inc. (same batch as test)
11/25/97-11/27/97
.
KC1 (Sigma "Ultra" Lot#76H10391)
343, 490, 700, 1000 mg/1
100% 580.2 mg/1 (490.0-700.0)
609.2 mg/1 (488.6-729.9)
CBI Stock Cultures 11/18/97-11/20/97 KC1 (Sigma "Ultra" Lot#76H 10391) 432, 720, 1200, 2000 mg/1
100% 929.5 mg/1 (790.9-1092.5) 1077.7 mg/1 (897.0-1258.4)
D-399
US00001580
CUMULATIVE DATA SUMMARY NPDES Permit #: N/A Oceana - ODU Feed S.
Ntvfyg c&i * y
Datepf:.:;-; Invdrtebrafe^^ft Te'st^'f?
2/12/97 <6.25
2/28/97 Invalid
3/12/97 17.7
3/20/97 19.5
3/26/97 33 9/30/97 8.1 11/3/97 12.5 11/18/97 23.3 11/25/97 9.7
LVeer5td0bLra%te&xi^t|^.MT^s#5fIn=MvaSeSSr5tiieSSb. rate^'^'7_-'^,*sNVeOrtEebCr%ate.?T\ 31
35
52 35 34 3.5 19.7 36.6 13.8
Supplement
D-400
'i
US00001581
COASTAL BIOANALYSTS. INC. NPDES STATIC ACLTE/SALTWATER
(Forni AMB48)
\ Effluii 1.0. Dllitv1a
Conimi C-l I0 C-2 *c> W 10
6.25 1-2 |0
2-1 \O 12.S 2-2
3-1 3 25 3-2 IO
4-1 G if 50 4-2
5-1 o 5 IX 5-2 1 J
0bs4irvar P6 Tirrm: 1M 55
DtaUyv2a
16
_ H i 2____ __ 2____ _ _ 3 ____ _ ____ ___ 2*___
i 0
o
___Q ____ 0
__ Q._____ .. * 0
l5 (o
fSuirnvailva*l V6
iT
M
Sourer
-6BF 0 0
Acelirreiion vsiter HWMASW \ 3 T Oth: | CL c*
Selinitv:
to ____ 3 &
__________
id Othac
Feedng dirinq t u t : Not fid O
A/ttie_ A a
Other.
C h * : 3 0 0 n iH
S O O n i Q . JOCOtti
Oth-
Test vofurre: 200 04 0 ,
2 5 0 -N
SOOtrt Other
No. radcata c
. frf *<
Phnto,riott I6L:80 0 . Oth:
Rm tfctron ttnpi no.5 ~ 1
I I 5 to n -SET UP: D m I
r< i> M A M
IH'I0
Tim* AninWs A d tM _ \ O O
S it Up Bv
INSTRUMENTATION USED:
Slim T him w ritif Nterier. A 1 Q Y3 D.O. M e t # 1 (SNE8014475) { 3 CP Digisense pH M et ID950O5S3) Sinorius RI 50P B rin c i (40020093) YS SCT 33 M et (H8016792)
0 YS D.O. M eur # 2 (SN91A02S329) n Coning 2 4 0 pH M et (SN5268)
SP P o tr a i M et (8S2360) S L Spesi) ATCRefncton>tlA36S)
Other
Ovy 0
Day 1
Day 2
C <5VJ.S .p* 1
30.
T t 3 0 -5 P O -'
? g- 1'
E 2 ;9t> .v ..... M
7 7 . 5"
3 POM
7 (2 3
4? ^ V
4 90-H
2a T
5 Po .3
3V. 2 o W
c %?\
7 . 9 3 7 . ??"
1 S .rt
7 - 5 7 .. . * f r<?
2 %0 7
7. VP- 7 77
(M
3 *7-17 7 ^ / . _
4 ~7.")a 7 - 7 /
'V *
5 7 .3 4
7 ,f?
JP.0'
C 1.t
??
77
t 7-7
7 0 _ L 2 ___
0.0. 2 7 ?
&.5 / 7
(tngfl) 3
7 -?
4 7 -?
3.S 7. 6 H-0 -7 7
5 7 .?
sC A1 L
2 Po
N 3 2*
4
50 2e
PG
P>0 TjO _ kAa 2, ____
*3 /"!/
5
Replicata9
trinai*:
5X) (a
____ _ --
2. S P ------
NOTES;________ ___________________________ __________
fon nl
3-ou o I n i^~o C 'ijc^.o
QiaJity Atturwcr
As Quriity Am*oHicarfor thb tast I cartify tfat no sigwRort ^wUbons from accaptad protocol cccumd dtaing m u r a im pact (u Jm oihar*a notad balow) and that tba cb u eeuratdy rofNet tha
tost it wm partonrad by CBI.
Cu H l l i l U
'
/
TEST ID :
H i ___ 13:1451
D-401
US00001582
EPA P R O B IT A N A LY SIS PROGRAM USED FOR CALCULATING L C /E C VALUES
V e rs io n 1.5
J R A 9 7 1 5 1 M BAHIA
C one.
N um ber E xp o sed
6 .2500 1 2 .5 0 0 0 2 5 .0 0 0 0 5 0 .0 0 0 0 1 0 0 .0 0 0 0
20 20 20 20 20
N um ber R esp.
4 14 19 20 20
O bserved P ro p o rtio n R esponding
0 .2 0 0 0 0 .7 0 0 0 0 .9 5 0 0 1 .0 0 0 0 1 .0 0 0 0
P ro p o rtio n R esponding A d ju sted fo r
C o n tro ls
0 .2 0 0 0 0 .7 0 0 0 0 .9 5 0 0 1 .0 0 0 0 1 .0 0 0 0
Chi Chi -
Square fo r Square fo r
(ta b u la r
H etero g en eity H e tero g en eity v a lu e a t 0 .0 5
(c a lc u la te d ) le v e l)
= 0 .1 2 0 = 7 .815
JR A 9 7 1 5 1 M BAHIA
E s t i m a t e d LC/EC V a lu e s a n d C o n f id e n c e L i m i t s
P o in t
L C /E C 1.00
LC/EC 5 0 .0 0
E xposure C one.
2 .7 5 7 ' 9 .702
95% C o n f i d e n c e L i m i t s
L ow er
U pper
1 .020 7 .510
4 .2 6 1 1 2 .0 0 7
8
$ S
D-402
US00001583
COASTAL BIOAN ALYSTS. INC. NPDES STATIC a CUTE/SALTWATER
(Form ACV4S)
D-403
TEST I.D.:_^ZM-33-L
EPA PR O B IT ANA LY SIS PROGRAM USED FOR CALCULATING L C /E C VALUES
V e rs io n 1 .5
JR A 97151 C VARIEGATUS
C one.
N um ber E xposed
6 .2 5 0 0 1 2 .5 0 0 0 25.0 0 0 0 50.0 0 0 0
100.0000
20 20 20 20
20
N um ber R esp.
1 8 18 20 20
O bserved P ro p o rtio n R esponding
0 .0 5 0 0 0 .4 0 0 0 0 .9 0 0 0 1 .0 0 0 0 1 .0 0 0 0
P ro p o rtio n R esponding A d ju sted fo r
C o n tro ls
0 .0 5 0 0 0 .4 0 0 0 0 .9 0 0 0
1.0000 1.0000
Chi Chi -
Square f o r Square f o r
(ta b u la r
H etero g en eity H etero g en eity v a lu e a t 0 .0 5
(c a lc u la te d ) le v e l)
0 .095 7 .815
JR A 9 7 1 5 1 C VARIEGATUS
E s tim a te d LC/EC V a lu e s a n d C o n f id e n c e L im its
P o in t
LC/EC 1 .0 0 LC/EC 5 0 .0 0
E xposure C one.
4 .7 1 8 1 3 .8 1 4
95% C o n f i d e n c e L i m i t s
L ow er
U pper
2 .3 8 5 11*298
6 .593 1 6 .8 3 6
D -404
US00001585
COASTAL BIOANALYSTS INC. EFFLUENT/DtLUTION WATER DATA <Fom tKDLi)
INITIAL (ARRIVAL) EFFLUENT CHARACTERISTICS:
'----- ----
Sample Date 1 " ( U f a 1_ _ _ _ L_ _ _ _ 1 ` 1 1 1
Temp. (C) 1 M
1
1
1
1
1
1
Conduct. (uMHOS) 1 / L i V 1
II
111
Salinity (g/kg) 1 ~
1
1
1
1
1
1
pH I ( , m
ii
11
Diss. Oxygen (mg/I) | t o. * | | | | | |
Tot. Chlorine (mg/I) 1/ h L 1 1 1 1 1
NH3-N (mg/I) 1 1 1
1
1
1
11
Hard (mg/I CaC03) 1 1 1 ^
1
1
1
11
AJkln (mg/I CaC03) 1I V e! 1 ! 1 1 1
Color/Appearance1 1 o> 1 1 1 1 1 1
Initials 1 f P 1 ! 1 1 1 1 1 1C-C!ear O-Opaque Y-YellowB-Brown BI-Black G-Green T-Turbid SI -Solids (S-SIighi M-Modcraie V-Veiy Much)
EFFLUENTADJUSTMENT/POST-ADJUSTMENT MEASUREMENTS:
Date l</tJ-tA-)l 1 1 1
Temp. (C) 0-^-V f i l l
Salinity (g/kg) 7 - 0 1 1 1 1
D O. (mg/1)
To . 1
1
1
1
Aeration Rate
--1
III
Aeration Time (min) | --
1
III
Adjust. D O. (mg/1) |
1________ !______ 1
1
pH 1 7 .3 , 1 I I 1
Tot Chlorine (mg/1) j ^ P 1 I 1 1
Initials
j R ____ !_______ ! . .
1
1
1 1 1 1 1 1' 1 1 1 1
1 1 1 1 1 1 1 1 1 1
ACUTE II II II II II II II II II II
DILUTION WATER CHARACTERISTICS:
Date Temp. (C)
| ulKfcnl | T o .r i
1 i
Conduct. (uMHOS) | Salinity (g/kg) j
1 1
1 1
PH |
Diss. Oxygen (mg/I) |
-lard (mg/1 CaC03) j
Alkln (mg/1 CaC03) |
Initials
|
'u 0
IA -
' p
1 1 1
1 i
1 1 I
1 i
DMW: HWM:
1 1 ., ii
II
1 1 1 II
1 1 1
11 ii
1 1 1 1 1 1'
1
1 1
1 1 1 1 1 1
ACUTE
II II II II II 1 1 II II
1
EFFLUENT ID.
__________ TEST ID: 'S '/L a Q ' l l Z /
D-405
US00001586
Toxicity Test Sample Chain of Custody (Please complete all information)
County__ NPDES#. Sample c< Type of s
Pipe/Outfall/Location. Instream Waste Cone
k
_(Composite): From Date. To Date__
Time_ Time.
Subsamples comprising composite:
fMj . iummhoer rr.------------_-_--_-_-
F**req*uency
.of
collection. n *n
Temperature o f sample in sample collection d e v rc e _0 ^ -
Volume.
Ftaal remperarure of effluen. -
Ts sample collection device c. 1 e
I t is required .ha. all samp.es
m p iC
i ce for s h i p m e n , ^
W .* ----------------
4
4c during ccoolllleeccttiioonn ppeerriioodd aanndd sshhiippmmeenntt ffcor
* roo free!.
,s the sample chlorinated?-- S -- dechlor,na,ed?_i. so, h o ------
IS tne bamp
. _ if ycs - limit (mg/L)--------------
Permit with interim chlorine limi ----------
Fielud r,,tr 1,7 . lot.
Friiecilad Tiuoutanl^Ri"e---sidu--al Chlorine-----------------
Field p ---- UZ
C rjrJ L C V P cA ^
Comments/Sample descnption__V2*_Y2J----------'
(Specify organisms)
------ ~ T 1
Method of shipment. "
^ --2 Prrint && Si ign Names
1. Received Relinquished by y -- -----
-Received by--
DaCc n,re
x /rfot-~
Z z a ^ tm T i m e _ ^ Z - ^ ---Time. <c//
FOR REED LAB USE ONLY
tpa? c1 ^ / 3 i7 l / /
Arrival Temperature.
_
Color
pH-- Salinity (ppO_
____ __ O d o r_
DO (mg/L). _____ Conductivity (pmhos/cm). TRC (mg/L)______ Method.
On ice?
b
D-406
3 e v 5 / '. S / 9
US00001587
O D U /O ceana Acute Effluent Toxicity Tests
M ysidopsis bahia & Cyprinodon variegatus
Sample I.D. A1 Contract No. N00187-93-D-9748 x Delivery Order No. 0235 J.O. No. 1912290, Item No. 0002BJ, 0002BH
PWCNo. 1381 11/24/97 1950 Sample No. JRA97-13442
Submitted to: Ms. Merrill Anderson-Ashcraft
Navy Public Works Center Code 930, Building Z-140
Norfolk, VA 23511
By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062
(804)642-0168
D-407
US00001588
iin i'.O C K A N A Sumpl^ I D. A l ,K 2 4 ,9 7 Sample 97-13442 Tesi ID: JRA97I53
Coastal Bioanalysts Inc.
I. TEST RESULTS
% Effluent Cfluceniraiicn
0 6.3 12.5 25.0 50.0 100
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
___________
Table 1. Summary Acute Test Results
Mean (%) Organism Survival
M. bahia
24-h
4SJi
C. variegatus
2Lh 100
48-h 100
oo oo oo
w
pH
100 100
oo
^H
100 100
oo
^H
100 95 100 85 100 55
100 100 100 95 100 100
>100%
N/A N/A <1.00
>100% N/A N/A <1.00
Acute Test Information:
Test Type: End Point. Test Start. Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity. Acclimation Temperature. Test Aerated?1: Aeration Initiation Date/Time..
M. bahia Test
48-h Static
Survival
11/25/97 1520
*
11/27797 1550
C hesapeake Cultures, Inc.
11/25/97
20 g/kg 18.9-20.8C
No N/A
C variegatus Test
48-h Static Survival
11/25/97 1525 11/27/97 1600
Aquatic Bio Systems, Inc.
11/22/97
20 g/kg
18.9-20.8C
No N/A
D-408
US00001589
COASTAL niOANALVSTS. INC. NPDRS STATIC ACLTOSALTWATER
(Form AMB4X)
\ Effluent
1.0.
Osy t 4 live
Controf
C-1 C-?
1-t 6 . 17
2-1 12.5 2 2
3-1 2S 3-2
4-1 50 47
5-1 100 5-2
0b**vr
Time;
1< [C
lo IO (O to l to 1 |0 lo IO
f
1*7^6
0ey2. tliv e
c
?
? P
'0
7 0
iO
rl
.7
9f. -*
>iO )tiH 0
O O
F m lt Si r m i
/
W
7>
Species: M ysidopsis b ahia
Source_____ -CD! ' g.< L
Hatch OatafTime:
h (jh i
Accmtion water. HWMASW W
Other
Seltrify:
Acdimtion Terr. (range);_
|& l
footing prior to tool: ittenoo , 3 < j Ottr______________________
Fetdng during test: Not fed
Attenta / A> / d 0rhec_
Chanter size: 300 of 0
500 of tOOOnf O th e r^ .
T n t votum; 200 nf 5 3 ,
250 nf [ ] 500 rH [ ) Other
Initial no. organism per i n f l e t t e
I *0
rfLNa n r f c m r
PSotopenort 161:80 0
Other;
flanAangetion tsm fate n o ~ I , ^
SET UP: Dto I I I J X 1 1 9 7 _ Tim Wafer Adkfcd 1
Tim Animals Added ( (?
Set Up By r t y ( ? --
INSTRUMENTATION USED:
Storn Trermm ter N u ito : ^ I
D T3 0.0. M ow 1 (SNE80J 475)
3. CP D iu r n o pH Motor [095005631 I] Sonoma R t6 0 P B I o (40020093)
3 TS SCT 33 Motor (H80I6792)
0 Y9 D.O. Motor 2 {9S1A026329)
Coming 2*0 H Motor (SN5268)
SPP0rtapH2 Motor (852350)
G2.S*rl> ATC Rofroctctmtor (A3S8)
OtSor
D-409
0r0____ Orr1____ Coy2
C Pe. 1 51' t>
T E
1 =?*>cj 3
P<?-3 Pi? 1
2e v 2e. y
P3
2o. 1 2e -1
4 tPf'-C J2.5.L> A-- ?
5 AO O a? o
V
C 9> ^ sto v 3 y
? ^y
:f
pH
2 l 'C 3 S p i Sj9
5". <?<?" ,7 /?
4 SM>7
X J y*-
5 yu-o
-r j
C ?.7 7 ?
7. 7
1 i . 9 7?S 7.7
0.0. 2 1 1
7G
(ml) 3 7 ? 7 ? 7. 7
4 7 7 77
7. 7
5
S ;c
A L I
.
i 2
. 7.7 7o I?C
-PO
7.7 ---1--fV---
l o 2c
PO } i-,
Pe
N 3 P * 30
<_jL
ftfVg) _ 4 Po> rx? 16
5 ^3D
flefieeteS P
f .(
Initiais: . - i -- f
. 5 Y)_
NOTES:
~h & ~7 S \ ^-ftOD '<-NIOTs'fc kocj 6 .
__ a___________________________________________________ _______
Quality A m n re a :
As Qtality A ssum es officer for this test t certify that no sipifiesnt teriafiora from sectored protocol ocevred during routine impactions (unfen otherwise noted below) end that the dita accm rety reflect the test as it was perfomwd by CBt.
Comments _
__ __
/
TEST I.D .:jO j^ _ 9 T j_ 5 ^
US00001590
COAST AJ. BIOANALYSTS. INC. NPDF.S STATIC ACUTE/SALTWATER
(Form ACV4R)
\ Efrluwnl
1.0 .
c-l C-2
1-1 6.25 r-2
. 2-1 12 5 2-2
3-1 25 3-2
4.1 50 -Z
5-1 too 5-Z
Ohsnvw. Tims:
0f.l VIrr* t "i
\^
10 .J
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(0
(0
(0 -
|C .
10
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Co .
Ojf 2 t Viw
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9 0
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10
0 10
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1(5
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1
final* [V e
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\ U V, rfC \v ,
Cvonnodon varieq^tus
Sckjt:
-6B h
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Acci."-TM -rater HWMASW
Other
1n
to
-----------------------Sriiraty O - Q ... rT O *3
16 Other
Fwdng dxing rest: Not fed 0
Artcma
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Cherie, to ,: 300
SOOrl 0
lo o o n n OtUr
T eetveW . 200 u Q
250 U [ 3
500 ni n
nth-
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Ffe. rcoTpotss:
,
Wnrooerioit 161:90 H Other
Ran<farnfnft terrrieta na^2 ~ ^
tyrilP- n.te U 1 3 - ' . 5 l 7 7 TirreW etttAtkW /
----------
T er. Arnrmls Added 1 L j ~ ^ ^
SetU&Bv f h j f t ...
INSTRUMENTATION USED;
item Thrmemetef Nurrber 1 YSI 0.0 Meter f 1 ISNE801 <475) F 3 . CP Olgraem pH Metef {095005631 ("H SwTonus P160P Balance (400200931 Y3 SCT 33 Mete, [H801S792)
E3 7 3 D.O. Me!, 2 IS7SIA026323)
Co n in g 2 4 0 (#t Meter (SN5268)
SP PwtbH2 Meter (BS2360) [ ^ Spartan ATC RefractcMmter (A366)
OeyO
Oey I
Day 2
C 2-0 H
IO
T
2 .3 0 M p 3 Aa
I tt ,? c c 7 o .t
4 J3 .1
5 >0
7 0 .2 '^ c .\
` c 2-i>. 0
2 P .Q
` f.f.
I e! 7 n -v
C tit
t C l
s r .o ?
1
> 7.
Z - 'S -
2 S3 o
pH
7 ..... % - > T
3 03
r. n
4 9> 1 X
S -A *
< r.
5 % 02
? A?
0.0.
(it^n
C 1 .1
1 1 .1
2 7 e?
3 7 .
4 7 .^
n < \ .... n s .. ,, 1 -1
h
7 .7
7. i 7. $ l- l 7- 6 7 . C,
5 7 * 1 7 .fc
s c ls>
A 1 to 2l
lo
N 3 2-0
7 -e 720 7JC
4 L /o
2*
-
2c ; 20
2 -0
T t f t ______
5 R ettoti*
. v=> 2
_ Z > _____
1 2k
Initials:
P O ..
^ 0 ......
NOTES:.__ ___________________________ j-----j-
YflP.al 5-tw^xj, rfcU cgi " I T .
UhlufVT^________ ________ ___
D O . h i cvt u r e o .'-a ) o
TEST I . D . : _ 2 _^2-1X D-410
US00001591
COASTAL BIOANALYSTS FNC. EFFLUENT/DILUTION WATER DATA (ForTMEFDLOl)
INITIAL (ARRIVAL) EFFLUENT CHARACTERISTICS:
__ ......... .
\Sample Date
U (uik)\
l o iTemp. (C) 1
i 1Conduct. (uMHOS) M i
1 !. !_ _ _ _ 1 1 1
! 1 1
1 ! I
i
1 1
1Salinity (g/kg)
-
1PH g .i 7
1 1
1 1
1
i
1 1
1 i 1 ! 1Diss. Oxygen (mg/1) Z/.o
I 1 1 1 1 1Tot. Chlorine (mg/1) ! / ) ( -
M a 1 1 1 ! !NH3-N (mg/1)
1 1 1 1 1 1 1Hard (mg/1 CaC03) i / y
1 1 1 ! 1 1AJkln (mg/1 CaC03) --/r--rt--tt------------- --- ---------
1 1 1 1 1 1 1Color/Appearance1 r i \
*
1 1 1 1 1 1 1Initials
PP
_
--
..
'l C-Clear O-Opaque Y-Yellow B-Brown Bl-Black G-Creen T-Turbid SI -Solids (S-Shghi M-Moderatc V-Very Muer
FFFI KENT ADJUSTMENT/POST-ADJUSTMENT MEASUREMENTS:
; Date Temp. (C) Salinity (g/kg)
11 1 zo.< 1I Q
111 111 1_ _ _ J________ L
1 1 1
1 1D O (mg/1)
7A
1
1
1
ACUTL
1 1I 1 1! 1I
Aeration Rate 1 --' 1
1 _L_______ !------------
1"
| J !_ _ _ _ ! !Aeration Time (min) -- 1_______ ________
------------ ------------------------- ---------- -
Adiust. D O. (mg/1) 1 --
1dH
1 1
11 J1_ _ _ _ J1 ! ! I------------ ------------!---------------------- -
1 1 1 1_ _ _ J !Tot Chlorine (mg/1) 1 1 1 1 1Initials . CP
________ ------------------------------ -----!
DILUTION WATER CHARACTERISTICS: DMW:
Date | / i /F7a ->I
I1
HWJVl:
1
ACUIC.
1I
! J L !Temp. (C) | => 1 ____ _______ ________ _______ --------- ---------------- ---------
_J 1Conduct. (uMHOS) [ --____ |______ ________1_______
____!------------------------------------
I _ _ J_ _ _ _ !Salinity (g/kg) 12^-^ 1kVs 1 1 i 1 1 IdH
1
|
-----------*
----------- ---
| 7 1 IDiss. Oxygen (mg/1)
l L !-________ ______ _______ !----------!------------ -----------
IHard (mg/l CaC03)
I
Alkln (mg/l CaC03) 1 ^ 1
foInitials
1
1
I 1 1
I 1 1
__ !1 ! 1-________ ------------ - - - - - -I - - - - 1 1I
EFFLUENT ID:
TST ID: D-411
5
I SX
US00001592
Toxicity Test Sample Chain of Custody (Please complete all information)
Fnriiity OJL P g tn W G y ) u*u>/ersv~frj
Address. tO<9rfo\VL-, v/A
County_
Pipe/Outfall/Location
NPDES#_
Instrcam Waste Cone
Sample'collected by (print&sign)
Affiliation
Type of sample ^ /Grab):
Date
7 Time 3 ' 3 3
~ /Composite): From Date '
Time.___________
To Date ______ Time ' ______
' Subsamples comprising composite:
Number_____-- ' Frequency of collection
Volume__________
'Temperature of sample in sample collection device ^ 0 &
Final temperature of effluent at sample collection point
'Is sample collection device chilled?
Is sample packed on ice for shipment?
'
`It is required that all samples remain at 0 - 4C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency (Do not freeze!)
Is the sample chlorinated? ^
dechlorinated? ^lVO If so, how?
_____________
Permit with interim chlorine limit?________ If yes - limit (mg/L)___H _______________
Field pH
Field Total Residual Chlorine. "
Comments/Sample description H 3 "
Type of test(s) to be performed
GHvfltlP , C /i/^ rn Q ^ O fi
(Specify organisms) \ f
Method of shipment
GfflX
Print & Sign Names
1. Relinquished by Received by / ? Relinquished by, Received by_
y; Date y ^ / ^ i Time________
Date U
7 Time ( ( Q.
-t Date
Time 4- - f i
c ts Date
Time / l (< &
\ 4 /i2 ?
FOR REED LAB USE ONLY
TRA# 7
Arrival Temperature______
Color_________________ _____ Odor____________'
pH_________ DO (mg/L). ___ Conductivity (pmhos/cm).
Salinity (ppt).
TRC (mg/L)______ Method_________
On ice?_ Solids.
D-412
US00001593
Coastal ioanalysts Inc.
ODU/Oceana Acute Effluent Toxicity Tests
Mysidopsis baha & Cyprinodon variegatus
Sample I.D.B1 Contract No. N00187-93-0-97,48^, s Delivery Order No. 0235-.' ' J O. No. 1912290, Item No. 0002BJ, 0002BH
PWCNo. 1384 11/24/97 2100 Sample No. JRA97-13445
. ,. Submitted to; Ms. Merrill Anderson-Ashcraft
Navy Public Works Center Code 930, Building Z-440 ; ,, Norfolk, VA 23511 "
By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062
(804) 642-0168
D-413
US00001594
ODI* <>CK.\NA Sam ple I.D. I3t \ i 24-97 Sample 97-13445 Tesi ID: JR.A97156
Coastal Bioanalysts Inc.
I. TEST RESULTS
% Effluent Concentration
0 6.3 12.5 25 0 50.0 100
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acute Test Results
Mean (%) Organism Survival
M. bahia
C. variegatus
244x 48-h
24-h 48-h
iop 100
100 100
100 100
100 95
100 100 100 75 100 c 45 95 0
100 95 100 100 100 100 100 100
"0.2% 32.8-49.6
Probit 2.49
> 100%
N/A N/A < 1.00
Acute Test Information:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?1: Aeration Initiation Date/Time:
M . bah ia Test
48-h Static
Survival
,
11/25/97 1650
11/27/97 1700 :
Chesapeake Cultures, Inc.
11/25/97
20 g/kg
18.9-20.8C
No
N/A
C variegatus Test 48-h Static Survival 11/25/97 1650 11/27/97 1715 Aquatic Bio Systems, Inc.
11/22/97 20 g/kg 18.9-20.8C
No N/A
D-414
US00001595
O D t : ( X T .A N A Sam ple I D . B l 1 1.24-97 Sam ple 97-13445 T o si ID : JR .A 9 7 I5 6
Coastal Bioanalvsts Inc.
Sample Collection Date/Time
11/24/97 2100
Sample Use Dates/Times
Aeration2 Rate/Time
11/25/97 1650/16503
.
n/a
Pretreatment/ Adjustment
HWM Salts
2Aeration often necessary to reduce sample oxygen concentration to 100% saturation after wanning.
3y/ bahiu C. variegatus test time.
Table 2. Effluent and Dilution Water 4 Chemical & Physical
Characteristics (Mean Value (Standard Deviation))
Parameter
Temperature (C)
Salinity (g/kg)
Conductivity (uMHOS/cm)
pH
Dissolved Oxygen (mgO)
Total Hardness (mg/1 as CaCO' )
Alkalinity (mg/1 as CaCOj)
Total Chlorine
(mg/1)
n h 3-n
(mg/1)
'
Initial Effluent
1.1
(-)
N/A
4670 (-)
8.32 (" )
10.8
(-)
176 (-)
242 (-)
<D.L. (-)
12.5 (-)
Adjusted Effluent
20.0 .
(--)
20
(-)
N/A
`
7.97 (--)
7.9 (-)
N/A f
N/A
N.D.
N.D.
Dilution W ater 20.5 (-)
20
(-) N/A
8.30 (-) 7.9 (-) N/A
N/A
N.D.
N.D.
4EffIucnt parameters measured upon receipt of sample in lab and after adjustment on day of use. Dilution water (Wimex hw-Marincmix in ASTM Type I deionized water) values measured after preparation of diluent on day of use.
D-415 .
US00001596
o u r OCEANA Sample I D n i I t. 24.97 .S.miple97-13445 Tesi ID: J R .W l 56
II. WATER QUALITY
Coastal Bioanalysts Inc.
Parameter Temperature
(C) D.O.
(mg/1)
pH
Salinity (g/kg)
Parameter Temperature
(C) D.O.
(mg/1)
pH
Salinity (g/kg)
Control
20.2
(0.3)
7.8
(0. 1)
8.11
(0.25)
20 (0)
Table 3. Water Quality Summary
M . baha Test
2%
20.3 (0.3)
7.8
(0.2)
S II (0.17)
20 (0)
Mean Value (Standard Deviation)
12J.%
2i %
50%
20.2 20.3 20.2
(0.2) (0.2)
(0)
7.8
(0. 1)
7.7
(0.2)
7.7 (0.3)
8.09
(0. 11)
8.09 (0.03)
8.11
(0.06)
20 20 20 (0) (0) (0)
100%
20.1 (0.2)
7.7 (0.3)
8.11 (0. 12)
20 (0)
Control
20.2
(0.3)
7.9
(0. 1)
8.17 (0.19)
20 (0)
C. variegatus Test
Mean Value (Standard Deviation).
6 3%
20.1
(0.4)
7.9
(0.1)
12.5%
20.1 (0.2)
7.8
(0.2)
25%
20.0 (0.2)
*' 7.8
(0.1)
50%
20.0 (0. 1)
7.7 (0.3)
8.15 (0.17)
8.14 (0.13)
8.13 (0.05)
8.10 (0.02)
20 20 20 20 (0) (0) (0) (0)
100%
20.0 (0. 1)
7.6 (0.3)
8.09 (0.08)
20 (0)
I5-4T6
US00001597
O D I \ O OE .\N A Sample I I). D l I I 2 + 0 7 Sam ple 9 7 -134+5 Text ID: JR A 9 7 I5 6
III. OUTFALL DATA SUMMARY
Coastal Bioanalysts Inc.
Test Date
9/30/97 11/3/97 11/25/97*
Table 4. Cumulative Data Summary - Acute & Chronic Tests (Tests conducted on this outfall by CBI)
Invertebrate
Acute Tests
Chronic Tests
LC50 T.U.
NQEC T.U.
72.0% 50.5% 40.2%
1.39
1.^8
2.49
N/A N/A N/A N/A N/A N/A
V ertebrate
Acute Tests
Chronic Tests
LC50 T.U.
NQEC T.U.
> 100% < 1.00 > 100% < 1.00 > 100% < 1.00
N/A N/A N/A N/A N/A N/A
Results submitted with this report
IV. QUALITY ASSURANCE
M. bahia - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
C variegatus - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
Chesapeake Cultures, Inc. (same batch as test) 11/25/97-11/27/97 KC1 (Sigma "Ultra" Lot#76H10391) 343, 490, 700, 1000 mg/I 100% 580.2 mg/I (490.0-700.0) 609.2 rng/1 (488.6-729.9)
Aquatic Bio Systems, Inc (same batch as test) 11/25/97-11/27/97 KC1 (Sigma "Ultra" Lot#76H 10391) 432, 720, 1200, 2000 mg/I 100% 1140.2 mg/1 (1019.5-1275.3) 1072.6 mg/I (887.0-1258.2)
D-417
US00001598
CUMULATIVE DATA SUMMARY NPDES Permit #: N/A Oceana - ODU > B-l
Date o f Invertebrate w Test" J G 5
2/12/97 >100 2/28/97 Invalid 3/12/97 >100 3/20/97 >100 3/26/97 >100 | 9/30/97 72 11/3/97 50.5 | 11/18/97 >100 1 11/25/97 40.2
Vertebrate.,' '
>100 >100 >100 >100 >100 >100 >100 >100 >100
Invertebrate^"- . Vertebrate .,
NEC-% ' - . NOEC%
..-V
1
1
J
i-
Supplement
D-418
US00001599
COASTAL b k >ANALYSTS. INC. NPDES STATIC ACUTE/SALTWATER
<Form A'MB4R)
\ fllluwl
t.o.
Oer 1 Irv
Der 2 9 live
Control 6.2S
C-l O C-2 _ J _ ! ________ M IC 1-2 1 0
10 >c lo 1 r-.
21 1 C 12.5 2-2 1 o
'p -- if
3-1 1 O _______ 25 3-2 ( o
4-1 1 o
s
50 4-2 ( O
ll
5-1 1 1? too 52
0
0"
Observer. ' Tirm:
f3 ib'TO
_______ 1 -7 0 0
Ml Survival
|0 ^
. cC
-\ o V
l '
u(
Hatch Dala^Tim*AccTirmtam Temp, (range):
Sourer
/t U t : 2-M rn
-eet (IC.
Sonify: O O
to P o X
^-
Of r t i r t j during test: N led
ArtoTM / x /d Other
300 ni 3
SCO ni
1000rH
O ll;__________ ^
Tmtvoli: K "* 0 .
2SO nd
SOOrrt Othor.__________ n i
Initisi no. o r q a n v m por m f c :
I T>
I*1- " I*" * 2 = --------
Photopooi 161:80 0
o tlx c _______ B w h m o lia n W rite no. 1 ~ 1, 7 -
------I2 7 UP: i t o . I I * > 9 l
TimtWr o * d * < l _ L y ^
Timt Aniimfe Addtd_ | O J ~ P
SrtUoBy r o / r . q -
INSTRUMENTATION USED;
S i m Thermorreter Number f i t t O * S 0.0. Meter 11 (SNE8014475) E 3 CP Diyiana cH Meter (09500563) Sartonus RI 60P 8 a l e (40020093)
rg SOT 33 Motor (HB016792)
0 Yg 0 .0 . M oi * 2 (SN91A026329) --1 [ Comin-g 240 (#1 Meter (S268) SP PortapW Molar 1852360)
13. Sporran ATC Rofractomatar (A366)
Other
C
T1 E
2 M
3
4
5
C
0.0. (mgR)
2 3 4 5
c
1 2 3
5
sC
A1
L 2
N3
(sAg) 4
5 Repfiatt *
Day 0
rP 0 S P .(
5Q . V
-P c 2. P e -C
% Tt
? > .3 o ?> \T
< 1 .0 1
S o*i
i n
n\
il
i.l
1.9
~) 9
7-9 do
Po
.P c
po per 20
Intuir J P
NOTES:
On 1 P * -e 3> x?
P c .o
7* A ,P o i
OC- 1 y .C| sr-0 5 9 ,..I
S l *> lt
IH 71
l . 't
i- H i- 1 ni
><3
po Do Do PO
P*
1
P
0ry7
jt* a
3 c/. V
2 6>- 5
2 c. S
7 0 .
p p -3 7 . 9-5
7. * 7
f-----V e / i^
fr c l
fr . fr
*20
7- 7 7- 6
7. 7 .
7 - >" -
7. y 7. y 2j
Z-O
2.COdj
7.0
2-0
_ _ a _______
-0
Tvn "7ft ot x? co i /-mOlcfcc bar.- -jQ .
QwBty Attirane*: A* Quality A m m i offic*r for this t**t I a rtify that no significant G tions frem tc a p ttd protocol oecwred dring n tifirm inspect (Wilms o O m n r m r e t t i Mow) and that tha * U aoewataly reflect the test as it was performed by C8I.
Contants
/T
m n z W
D-419
TEST I D.
T T fi-.'V
US00001600
r E FR U ENT/D<UjnO>. WATER DATA ,f-- COASTAL BIOANALYSTS I N C . __________________ _________-________ -----------------------
I-- - ' r
I * I fa')\ _____ ____ ___ _ '
r
EFFLUENT I D : _ iia J 5 ^ - i i l
US00001601
Toxicity Test Sample Chain of Custody (Please complete all information)
Facility_ Q \< p p T w i w i m Address. P Q r j b l l V ' A
County.
Pipe/Outfall/Location.
NPDES#.
Instream Waste Cone
Sample'collected by (print&sign)______________________ _A_f_fi_l_ia_t_io__n________
Type of sample ' S (Grab):
Date
Tirne^ 1 ^ * 3 ^ * 1
.(Composite): From Date.
Time.
To Date___
Time
Subsamples comprising composite:
Number_________* Frequency of collection.
Volume
"Temperature of sample in sample collection device 0 Cs
Final temperature of effluent at sample collection point
`Is sample collection device chilled? *y2S Is sample packed on ice for shipment?
'It is required that all samples remain at 0 - 4C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency (Do not freeze!)
Is the sample chlorinated?
dechlorinated?___H If so, how?______ ~ ___________
Permit with interim chlorine limit?
' If yes - limit (mg/L)------------ ---------------------
Field pH % -^*7
Field Total Residual Chlorine____________
Comments/Sample description 131 __________________ ;______________________________
Type of test(s) to be performed. M
Q n rin V , C r K Q g ^ O v l
(Specify organisms)
v/(^n
Method of shipment. P c f r f e r ^ Vtm ODIA
________________
Print St*S,ign Names
1. Relinquished by.S k * .
Date ^ ^ 9 * 5 l f l Time_____________
Received bv / /
Date f i 'Srf'T? Time f / O 'C
Relinquished by.
P.Received bv
A a,<.o (
Date
Time
^
_ _ _ _ _ Date f t - 9 ^ - 9 1 Time / 9 / _
FOR REED LAB USE ONLY
JRA#_
Arrival Temperature___________
Color___________________________ Odor__________________
pH__________ DO (mg/L)________ Conductivity (pmhos/cm).
Salinity (ppt)______ _ TRC (mg/L)______ Method__________
On ice? Solids.
4n^
0 \^
----
D-421
n tv
US00001602
D-422
US00001603
Coastal B ioanalysts Inc.
O D U /O ceana Acute E ffluent Toxicity Tests
M ysidopsis bahia & C yprinodon variegcihis
Sample I.D. B2 Contract No. N00187-93-D-9748
Delivery Order No. 0235 J.O. No* 1912290, Item No. 0002BJ, 0002BH
PWCNo. 1385 11/24/97 1945 Sample No. JRA97-13446
Submitted to:
Ms. Merrill Anderson-Ashcraft Navy Public Works Center Code 930, Building Z -140 Norfolk, VA 23511
*
By: Coastal Bioanalysts, Inc.
P.O. Box 626 Gloucester Point, VA 23062
(804) 642-0168
D-423
US00001604
ODI 'O C H A N A Sample I D. B2 11,24 97 Sample 97-13446 Test ID: JRA97157
Coastal Bioanalysts Inc.
I. TEST RESULTS
% Effluent Concentration
0
. 6.3 12.5 25.0 50.0
100
48-h LC50: 95% Confidence Limits Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acute Test Results
' Mean (%) Organism Survival
M . bahia
C. variegalus
24-h 48-h
1:P0 100 100 95 100 80 100 85 100 55 65 0
2di 48-h 100 100 100 95 100 100 100 85 100 85 95 20
42.1% 32.7-54.3 Trimmed Spearman-karber
2.38
72.6% 63.8-82.7 Trimmed Spearman-karber
1.38
A cute T est Inform ation:
Test Type: End Point: Test Start: Test End: Organism Source: Hatch/harvest Date: Acclimation Salinity: Acclimation Temperature: Test Aerated?*: Aeration Initiation Date/Time:
M . bahia Test
48-h Static
Survival
*
11/25/97 1655
11/27/97 1730
Chesapeake Cultures, Inc.
11/25/97
20 g/kg
18.9-20.8C
No '
N/A
C variegalus Test 48-h Static Survival 11/25/97 1710 11/27/97 1740 CBI stock cultures 11/17/97 20 g/kg 18.9-20.8C No N/A
D -424
US00001605
O O r OCKAN.\ Sample I.D. B2 *1.24 97 Sample 97-13446 Test ID: JRA97157
Coastal Bioanalysts Inc.
Sample Collection Date/Time
11/24/97 1945
Sample Use Dates/Times
11/25/97 I655/I7103
Aeration2 R ate/Tim e
n/a
Pretreatment/ Adjustment
HWM Salts
2Aeration often necessary to reduce sample oxygen concentration to 100% saturation after wanning. 3_V/. buhia C. variegatus test time.
s
Table 2. Effluent and Dilution W ater4 Chemical & Physical
Characteristics (Mean Value (Standard Deviation))
`Parameter
Temperature (C)
Salinity (gAg)
Conductivity (uMHOS/cm)
PH
Dissolved Oxygen
(mg/1)
Total Hardness (mg/1 as CaC03)
Alkalinity (mg/1 as CaC03)
Total Chlorine
(mg/1)
n h 3-n (mg/I)
Initial Effluent
1.0
(-)
N/A
4900 (-)
8.20
(-)
10.9 (-)
176 (-)
260 (-)
<D.L. (-)
17.5 (-)
Adjusted Effluent
20.2
(-)
20
(-)
N/A
'
Dilution W ater
20.1
(-)
20
(-)
N/A
7.91 8.31 (-) (-)
8.0 7.9
(-) (-)
N/A N/A
N/A N/A
N.D.
N.D.
N.D.
N.D.
^Effluent parameters measured upon receipt of sample in lab and after adjustment on day o f use. Dilution water (Wimex hw-Marinemix in ASTM Type 1 deionized water) values measured after preparation of diluent on day of use.
D-425
US00001606
O D IM K '-.VNA Sample I D. B2 I J.2-.97 Sam plj 97-134-16 Tosi ID: JRA97157
Coastal Bioanalysts Inc.
H. WATER QUALITY
parameter Temperature
<C) DO.
(mg/1)
pH
Salinity (g/kg)
Parameter Temperature
(C) DO.
(mg/1)
pH
Salinity (g/kg)
Control
20.1 (0.2)
7.8
(0.2)
8.06
(0.21)
20 (0)
Table 3. Water Quality Summary
M. bahia Test
Mean Value (Standard Deviation)
63%
20.2 (0.2)
7.8
(0. 1)
8.04
(0.21)
20 (0)
12.5%
20.3
(0. 1)
7.8
(0. 1)
8.05 (0.15)
20 (0)
25%
20.3
(0.2)
7.7
(0.2)
8.01 (0. 12)
20 (0)
5fi%
20.2 (0. 1)
7.7
(0.2)
8.01
(0.08)
20 (0)
100%
20.1 (0.2)
7.6 (0.3)
8.10
(0.14)
20 ' (0)
Contrai
20.0
(0.3)
7.8
(0.2)
8.09
(0.22)
20 (0)
C. variegatus Test
Mean Value (Standard Deviation)
6 3%
20.0
(0.4)
7.7
(0.2)
8.04
(0.22)
20 (0)
12.5%
20.0
(0.3)
7.7
(0.2)
8.04 (0.18)
20 (0)
25%
20.0 (0.2)
*7.7
(0.2)
8.03
(0. 12)
20 (0)
50% .
19.9
(0. 1)
7.6 (0.3)
8.00
(0.13)
20 (0)
100%
20.0 (0. 1)
7.5 (0.4)
8.05 (0.13)
20 (0)
D -426
US00001607
O D I' OCKANA Sample I.D. 132 11.24 9 ? Sam ple *>7-13446 Tesi ID: JR.A97137
Coastal Bioanalysts Inc.
III. OUTFALL DATA SUMMARY
Test Date
9/30/97 11/3/97 11/25/97*
Table 4. Cumulative Data Summary - Acute &. Chronic Tests (Tests conducted on this outfall by CBI)
Invertebrate
Acute Tests
Chronic Tests
I.C50 T.U.
SQE.C IAL
66.0%
50.0% 42.1%
1-52 2.00
2.38
N/A N/A N/A N/A N/A N/A
V ertebrate
Acute Tests
Chronic Tests
T.C50 T.U.
NQEC U L
> 100% < 1.00 > 100% < 1.00
72.6% 1.38
N/A N/A N/A N/A N/A N/A
` Results submined with this report
IV. QUALITY ASSURANCE
M. bahia - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
C variegatus - Acute Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
Chesapeake Cultures, Inc. (same batch as test) 11/25/97-11/27/97 KC1 (Sigma "Ultra" Lot#76H10391) 343, 490, 700, 1000 mg/1 100% 580.2 mg/1 (490.0-700.0) 609.2 mg/1 (488.6-729.9)
CBI Stock Cultures 11/18/97-11/20/97 KC1 (Sigma "Ultra" Lot#76H 10391) 432, 720, 1200, 2000 mg/1 100%
929.5 mg/1 (7 9 0 .9 -1 0 9 2 .5 )
1077.7 mg/1 (8 9 7 .0 -1 2 5 8 .4 )
D-427
US00001608
[Date o f '
I 2/12/97 >100 | 2/28/97 Invalid | 3/12/97 >100
3/20/97 >100 3/26/97 >100 9/30/97 66 . 11/3/97 50.0 11/18/97|93.9 11/25/97[42.1
CUMULATIVE DATA SUMMARY NPDES Permit #: N/A Ocana - ODU B-2
VertebrateV:--^ "ci;--3* Invertebrate:-;
L 50% ~'X --[
NOC %
>100
>100
>100
>100
>100
>100
>100 j>100 72.6
....
Vertebrate . .w."y.* NEC% - ' `- Z
"------------- ---'
1--------------------- !
i
11 1
i
Supplem ent
D-428
US00001609
COASTAL niOANALYSTS. INC. NPDES STATIC ACLTE/SALTWATER
(Form AMB4R)
* tIMuent
1.0 .
D ari Irv*
C-l / 5 C-2 t o l-l (
6.25 1-2 ( 0
2-1 ( 0 12.5 2-2 ( o
3-1 25 3-2
4-1 50 4-2
5-1 100
5-2 Obw'vwc
: . Tirre;
to (O
1V
(0 )
to
\5 S -S
Oar 2 Lm
C
/D
'Jr
10
7
/
t
7 -
6
0. 0 So
11 50
ft* * Survival
I
lE C
/
<Dr
</
?
C
Specm e M ysidopsfc b ahia
Source;______ -G 8H
Hatch DatdTima:
J l / 7 7 I97
Accorrei ton water HWM ASW \ 3 T Other_______________ Salinity r D C )
AcdiTMtton Tenp. (range):_ Facing poor ro twt; Arterria , 3 .
TO /d Other
^ ________
Feedng duong test; Not fed
Affama / A /d Othec__
Chenier stre: 300 rri 0
500 rH
1000 tW
0t h e c _
Test w ttm e: 200 id i
250 rd
500 id Q
0 th e r_
id
Initial no. organsin per replicata: / I ~D
Mb. radicate:
Hetoperietfc 1 6 L 0 O 0
Other:
Ii SETUP: Paie I I I - 3 * S l ? 7
33BawAmiration temprata no. ^
Tirm W atar Adder)
.U
Mi P&
Tima AnirrWs Added_ INSTRUMENTATION USED:
L ,S
'Si f ?
Set Up(hr f o l f
Slam Tle ftnometer N w iter /^ - I I ] S I 0 .0 . M iter 1 (SNE8014475) 2 . CP O dierna pH Meter [09500563] Sm nm s R160P Baiava (40020093) YS SOT 33 Meter (H8016792)
0 T S D.0. Melar 1 2 (SN91A026329) Coming 240 H Meter (SW26B)
SP P o r ta r Malar (852360) ^ Spartan ATC Refiactometer (A366)
Other
C
TI
E2
M P3
4
S
C
1
2
pH 3
4
5
C I
0.0. 2
(rr^l)
3 4
5
SC
A1
1 .2
N3
4
S
Bed a bove ma
Initiala:
OmrO_________ Deyl
Dar 2
2y
Pc v
S **-
P50.I Ie! -
K -3 &
SPI
1 on
^ .O O
7c /
Pl ZOO-
2 -b J I f lJ 7 .9t>
Sc5L
3 -0 7 9 3 .( 9 7 -T
Z
Pc 2Jo. 3 2c' i
2 t? 3
s
7-f T/ 7 7 6
7 ? !?
7 7 r>
y- "'
7. 3 b '
7. f/
7 . S - i-
*//r
7
& ' 6 / 7
7c
7?
7-?
7 -?
77
7 .?
7 -7
7 -7
7-G
7 -G
3 -{f
1 .7
7- 7
7,
77 ~f
Oc Q -G pb
PC P* Pt
M, TLC-
= 70
20
LO Lb
7.0
6
P a
5U>
it
1 2. 7 *
^ 6 JLL-- , i o
NOTES:
fe. ~7-Cl n 4 3-fc-CO i -T I 0) ^yXs^O.
I
Quaty k n x r o t a r .
As Quaty Aisurtnca officer for this test 1certify that no K^aficant Aviation from accepted protocol occurred dring routine rmacwns (into* otharwna noted below) and that the A ts scctrvtefy reflect the ta it s it was perfonied by CBC
Gonnanti
/ iZ
*y
/
77777/
/
Ss b ? . --
tr
^n
-
ft)
0*
D -429
TEST I D.: ZTA
US00001610
r i m m e d s p e a r m a n - k a r b e r m e t h o d , v e r s i o n 1 . 5
DATE:
1 1 /2 5 /9 7
TOXICANT : B2
S P E C IE S : M BAHIA
RAW D A T A :
------------
Concentration ( %)
.00
6 .2 5 1 2 .5 0
2 5 .0 0
5 0 .0 0
100.00
T E S T NUMBER: J R A 9 7 1 5 7
D U R A T IO N :
Number
E xposed 20 20 20 20 20 20
Mortalities
0 1 :4 3 9 20
SPEARMAN-KARBER TRIM:
5 .0 0 %
SPEARMAN-KARBER E S T I M A T E S :
LC50:
95% LOWER C O N FID EN C E:
95% U PPER CON FID EN CE:
4 2 .1 2 3 2 .6 5 5 4 .3 3
48 H
--
a d j o s t m e n t s Rw e r e T m a d e S S S r- S S S
E f p j
D -430
US00001611
COASTAL BIOANAI.YSTS. INC. NPDKS STATIC ACl T E SALTOATR
(Form ACV4)
% EMuani . 1.0.
01 C-2 M 6.?5 1-2 21 17.5 2-2 3-1 25 3 2 4.1 50 4-2 5-1 100 5-2 O b trvtc. Tin*:
Oey I t live
'0 i0
lo lo
lo IO
io
(0
to
IO IO
l
fc >
i~ ? 0 0
liv
I
lo
... I O -- 1 _________
1C
IO
FW % Survtvd
17 \0 O
<r
_ _ J Z _________ ET .
t-ea. 7 V
~ 70
*
n .w o
s 07
w
soect: w i Hatch DatafTirr*
/ M In
C8I
/ i 2 _____________
Acclirratioft water HWM itr i_ J w "1*-------------- --------
is.n
to r ? 0
Feeling dunng test: Not (ed 0
Arten.-------------- i Other------------------------
C W , .: 3 00 *
500* 0
'0 0 0 *
niO t f- r -----------------
I * O T ri
2 5 0 *1 3
500*
niO rt ;-----------------
I n t * no. ngansira pW r e tto le :
I D -----------
O
ret,ic" , " : --------------------- '
fto to p rn o d 161:80 a
Q th r
fUndwTWion teiT^at# no.
SET UP: Dt_ u h '} W *7_ TiTM Water A A M _ ( C> H<
lu i Aranwb AAfcJ / ^ ^ ______ S r t u , B Yf 2 _ t e L _
INSTRUMENTATION USED:
/ }Steffl ThonramBter M irte e
1______
VS D O. Meter 1 1 (SNE0O14475)
El CP t t g " pH M e w (03500563)
S a n t o RI 60P Balance (40C20093)
r g SCI 33 Meter (H8016792)
t a 0.0. M e t 2 (SW1A02S329)
pH Com' ^ 240 M e t (SM268)
SP P o r t M et 1852350) E J Spurten AIO Refractwmter (A366)
Other
0>r0
Dvyl
0*y2
C P -0.Y
Q <i
f? .*
T , P6S
/? .< !
E2
1 7 .7
M
3 M )J
PCJ
1 7 .7 ,7 .? ;? .fr
4 P O .?
p : .0
(U
5 Po.O
% C .O
C 1
.
2 3 4
5
C
1
D.O. (trnfl)
2
3
4
5
sC
A1 l t2
N3
4
5 Rem ota A
' Irath:
4 -3 1 1 7%
"7-7 5 9 OD
7 .0 1
9 ,.\\ nel n u it
< 3 .0 ^ 9 ! 35 .-
1 Ci n< \ iT n \ J ____ ? ._
? s. O. 7 *7 7 "7. k `7 t 7 -7 ; 2 o>
to 2 -0 L -i>
2_ff 2 .^
P&
1 ~~F ~
OC 7 .... f --
7- 7S 7 3 7 ... 7 . S C 7 - 7 7 - <? Ci 7 .< 7 7
l.L 1. b
i 7 .7 77 7 . j;-- /e * cv CO
70
to
co 1
70
NOTES:
SpoQiO-l
Uh lltPYlP
, rtd
_____
i lfS +
>* 7 .1 g l m ai) to t ac - T v - ^ -
D-431
TEST I.D.: H a ^
US00001612
RIMMED SPEARMAN-KARBER METHOD- VERSION 1 .5
DATE:
1 1 /2 5 /9 7
TOXICANT : B2
S P E C IE S : C VARIEGATUS
RAW D A T A : --------------------
C o n c e n tra tio n (%)
.0 0 6 .2 5 1 2 .5 0 2 5 .0 0 5 0 .0 0 1 0 0 .0 0
T E S T NUMBER: J R A 9 7 1 5 7
D U R A T IO N :
N um ber E xposed
20 20 20 20 20 20
M o rta litie s
0 .1
0 3 3 16
48 H
SPEARM AN-KARBER T R IM :
. 2 0 .0 0 %
SPEARM AN-KARBER ESTIM A TES :
LC50:
95% LOWER CO N FID EN CE:
95% U PPER CONFIDENCE:
7 2 .6 2 6 3 .8 1 8 2 .6 5
NOTE:
M ORTALITY PR O PO R TIO N S WERE NOT MONOTONICALLY IN C R E A S IN G . A D JU STM EN TS WERE MADE P R IO R TO SPEARM A N-KA RBER E S T IM A T IO N .
D-432
US00001613
COASTAL BIOANALYSTS'INC. EFFLUENT/DILUTION WATER DATA (Fom EFDL0195)
INITIAI.ARRTVAL) EFFLUENT CHARACTERISTICS:
...
...
Sample Date 1 u / m A 1 i. 1 ' I 1 1
Temp. (C) 1 /. n 1_______ J ______ _J-------------
1
Conduct. (uMHOS) 1 4 ^ 0 0 1 1 I
11
Salinity (g/kg) i -
1
1
1
.
1
1
pH 1 $ . v > 1
1 ..
11
Diss. Oxygen (mg/1) 1 O P \ i 1 1
11
Tot. Chlorine (mg/1) U f i . L 1 1 -
I1
NH3-N (mg/1) 1 n 5 1 1 1 - -
1
Hard (mg/1 CaC03) i n \ o
1
1
1
1
Alkln (mg/1 CaC03) 1
III.
1
Color/Appearance1 1 c &
1
1
1
11
'l
c .c
Initials ,eaf OOpaque
\/j
Y-Yellow
1 B-Brown
Bl-Black
1 G-Green
1 T-Turb.d
SI
-Solids
(S-Shght
1 M-Moderaie
1
v-very much;
.
EFFLUENTADJUSTMENT/POST-ADJUSTMENTMEAbUKlMvie.ni ia.____________________________ _
,, . ..'iDate
1 Ii/t x /atI
1
1
1 -------- !------------- ----------- -------------- -
Temp. (C) 1 7 0. V 1
1________ 1
i-----------!------------- !------------- -------------
SaJinitv (e/ke) 1 -- 1
1
D.O. (mg/1) I s o 1
1
Aeration Rate 1 -
1
1
Aeration Time (min) I ' l l
___ !________ !-----------'----------------------------------
1 1 - 1 1 II .._ 1 1 1 1 II
1 1 1 1 II
Adjust. D O. (mg/1) | --- 1 ______j________ !________ !------------- !----------- L-------------------- -
dH I n .'S 1
Tot Chlorine (mg/1) 1 H b
^ Initials
\ fa
1 1
\
1 __ !_______ L---------- !----------- !----------- ---------- -1 1 1 - 1 1 II 1 1 1- 1 1
DILUTIONWATERCHARACTERISTICS: DMW: HWIYI.
Date |H/i </<->!
1 !. ^ 1
II
Temd. (C) 1 io . i 1 1 ! 1 1 1 II
Conduct. (uMHOS) | - ' |
1________ !_______ 1-_______ L-------------!---------------------"
Salinity (g/kg) | 2. t> 1 ____ I_______ !_______ !_______ !----------!--------- ----------- -
oH I <C T l ! 1 1 __ !_______ L---------- !---------- ----------Diss. Oxveen (mg/1) 1 1 A 1 1 1 1 II
Hard (mg/1 CaC03) |
| _____ |______ J _______ !_______ !----- ---- !----------- -----------
Alkln fma/l CaC03i 1 " 1 1 1 ___ !_______ L----------J--------- ------------
. Initials
1 fo
1
11
1
1
1
II
1
SE F F L U E N T ID: , a
\ r.__________ T E S T r o : -------- . 1 4 -A
D-433
,,
US00001614
Toxicity Test Sample Chain of Custody (Please complete all information)
p o lity Q \ d
Address
V h fw u jQ X U i \/-e rs H M
/ Vi/A^A oo jat T^o2/SO\
J
County. NlNPr.UDCE.OSr#f____________________________ Sample collected by (print&sign) V
Pipe/Outfall/Location
uInstreamw Waste* C on e-.---------------------------------------------
----- Affiliation O & A .
UMo
Type of sample
^ .((G'Grrab')):
Date ^ / J ' AA '?l
.(Composite): From Date.
To Date__
Time .'I Time. Time.
^
Subsamples comprising composite: Number____________ Frequency of collection.
Volume.
`Temperature of sample in sample collection device--ClQ.--Cf
Final temperature of effluent at sample collection point .
"Is sample collection device chilled?
Is sample packed on ice for shipment?--
--
`It is required that all samples remain at 0 - 4C during collection period and shipment for
data to be accepted by the appropriate Regulatory Agency (Do not freeze!)
Is the sample chlorinated?
dechlorinated?-------- If so, how?-----Z H --------------------
P erm it with interim chlorine limit?
'___ If yes - limit (mg/L)-------------------------- --------
Field pH % . a O
Field Total Residual Chlorine------IZ----------
Comments/Sample description
,-------------------------------------------------------------------------- -
Type of test(s) to be r r f n n w d M p i g t e V i r i ^ p .
(Specify organlisms) V (m ! Pj& OiHA^------------------------------------ --
Method of shipment
0:
Print & Sign Names-
1 . Relinquished bv Received by S**/j * s>O
t O ^ p ate. '`/ t f S / t n Time.
*fi/) * -- Date-- i(tf / A
-- TTiimmee. f ( O S~.
Relinquished b ~ Received bv T *
I O ________ _
D a tz _ L --l4" 2 Time
Date N
Time
FOR REED LAB USE ONLY
JR A#
Arrival Temperature.
CoIor________________________________ Odor_____
pH__________ DO (mg/L)________ Conductivity (pmhos/cm).
Salinity (ppt)_______ TRC (mg/L)______ Method
On ice?.
D-434
ODU/Oceana Acute Effluent Toxicity Tests
Mysidopsisbahia&Cyprinodonvariegcitus
Sample I.D. B3 Contract No. N001S7-93-D-9748 * Delivery Order No. 0235 J.O. No. 1912290, Item No. 0002BJ, 0002BH
PWCNo. 1386 11/24/97 1900 Sample No. JRA97-13447
Submitted to: Ms. Merrill Anderson-Ashcraft
Navy Public Works Center Code 930, Building Z-140
Norfolk, VA 23511
I
By: Coastal Bioanalysts, Inc. P.O. Box 626 Gloucester Point, VA 23062
(804) 642-0168
D-435
US00001616
mi oG'FANA Sample I D. B3
ID:S a m p le 9 7 - 1 3 4 4 7 T e s t
JR A 9 7 1 58
Coastal B ioanalysts Inc.
I. TEST RESULTS
% Effluent rnnntratiou
0
6.3 ' 12.5
25.0 50 0
100
48-h LC50: 95% Confidence Limits: Calculation Method: Toxic Units (T.U.):
Table 1. Summary Acute Test Results
Mean (%) Organism Survival
M . bahia
24ih
10
48-h
100
100 90
100 95
100 95 100 95
10 0
C. variegatus
24-h
100 100
48-h 100
100
100 100
100 100 100 100 100 100
69.1% N/A Trimmed Spearman-karber
1.45
>100% N/A N/A < 1.00
Acute Test Information:
Test Type:
End Point:
Test Start:
'
Test End: Organism Source:
Hatch/harvest Date: Acclimation Salinity:
Acclimation Temperature:
Test Aerated?1: Aeration Initiation Date/Time.
M. bahia Test
48-h Static Survival
11/25/97 1720 11727/97 1750
CBI stock cultures
11/21/97
20 g/kg
18.9-20.8C
No N/A
C variegates Test
48-h Static Survival 11/25/97 1725 11/27/97 1800 CBI stock cultures 11/17/97 20 g/kg
18.9-20.8C
No N/A
(
D-436
US00001617
O D i;.()C i:.\N .\ Simple I.D. B3 'l l .'24/97 Sam ple97-13447 Test ID: JRA9715X
Coastal Bioanalysts Inc.
Sample Collection
Date/Time
.
11/24/97 1900
Sample Use Pates/TuMS
11/25/97 1720/17253
Aeration2 Rate/Time
n/a
Pretreatment/ Adjustment
HWM Salts
-Aeration often necessary to reduce sample oxygen concentration to 100% saturation after warming. 3.U bahia/C. variegatus test time.
Table 2. Effluent and Dilution W ater4Chemical & Physical
Characteristics (Mean Value (Standard Deviation))
Parameter
Temperature (C)
Salinity (g/kg)
Conductivity (uMHOS/cm)
pH
`
Dissolved Oxygen
(mg/1)
Total Hardness (mg/1 as CaC03)
Alkalinity (mg/1 as CaCOj)
Total Chlorine
(mg/1)
n h 3-n
(mg/1)
Initial Effluent
0.9 (...)
N/A
4780 (--)
8.22
(-)
11.0
(-)
176 (-)
244 (-)
<D.L. (-)
18.5 (->
Adjusted Effluent
20.8
(...)
21
(-) N/A .
8.01
(-) 7.9 (-- ) N/A ; N/A
N.D.
N.D.
Dilution W ater 20.9 (-)
20
(--) NVA
8.34 (--) 7.9 (~) N/A
N/A
N.D.
N.D.
^Effluent parameters measured upon receipt of sample tn lab and after adjustment on day of use. Dilution water (Wimex hw-Marincmix in ASTM Type I deionized water) values measured after preparation of diluent on day of use.
D-437
US00001618
ODI VOCE.\NA Sample ID . B.l I l 24*97 Sample 97-13447 Test ID: JR .\97138
II. WATER QUALITY
Coastal Bioanalysts Ine
Parameter Temperature
(C) D.O. (mg/1)
pH
Salinity (gAg)
Parameter Temperature
<C) DO.
(m g /1)
PH
Salinity (gAg)
Control
20.3 (0.4)
7.9 (0)
8.04 (0.27)
20 (0)
Table 3. Water Quality Summary
M . baha Test
Mean Value (Standard Deviation)
r
20.2
(0.4)
7.8
(0. 1)
7 93
(0.37)
20 (0)
U J%
20.3
(0.3)
7.8 (0. 1)
7.84 (0.35)
20 (0)
25%
20.2
: (0.3)
7.8'
(0.2)
8.00
(0.17)
20 (0)
50%
20.1 (0.2)
7.7
(0.2)
8.05 (0.06)
20 (0)
100%
20.1 (0. 1)
7.7 (0.3)
8.12
(008)
21 (0)
Control
20.2
(0.3)
7.9
(0-1)
8.06 (0.27)
20 (0)
C. variegatus Test
Mean Value (Standard Deviation)
6.3%
20.2
(0.3)
7.8
(0. 1)
12.5%
20.1 (0.2)
7.8
(0. 1)
25%
19.9
(0. 1)
. 7.S
(0.2)
50%
20.0 (0. 1)
7.8
(0.2)
8.07 (0.24)
8.05
(0.20)
7.97 (0.18)
' 8.04 (0.07)
20 20 20 20 (0) (0) (0) (0)
100%
20 0 (0-1)
7.8
(0.2)
8.07 (0.08)
21 (0)
D-438
US00001619
ODI -OC!-;.\N A Samplet.!}. B3 11.24-97 Sam ple97-13447 Tesi ID: JRA97I5R
III. OUTFALL DATA SUMMARY
Coastal Bioanalysts Inc.
Test Date
9/30/97 11/3/97 1 1/25/97
Table 4. Cumulative Data Summary - Acute & Chronic Tests (Tests conducted on this outfall by CBI)
Invertebrate
Acute Tests
Chronic Tests
LC50 T.U.
NOEC T.U.
65.0% 45.0% 69.1%
1,54
2.22
1.45
N/A N/A N/A N/A N/A N/A
Vertebrate
Acute Tests
Chronic Tests
LC50 T.U.
NOEC T.U.
> 100% < 1.00 > 100% < 1.00 > 100% <1.00
N/A N/A N/A N/A N/A N/A
` Results submitted with this report
IV. QUALITY ASSURANCE
M. bahia - Acute Reference Test Dates; Reference Toxicant: Test Concentrations; Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
C. variegatus - Acute . Reference Test Dates: Reference Toxicant: Test Concentrations: Control Survival: LC50 Reference test (95% C.L.): Control Chart LC50 (95% C.L.):
CBI Stock Cultures 11/20/97-11/22/97 KCl (Sigma "Ultra" Lot#76H10391) 343, 490, 700, 1000 mg/1 100% 574.8 mg/1 (532.7-620.2) 610.6 mg/1 (488.4-732.8)
CBI Stock Cultures 11/18/97-11/20/97 KCl (Sigma "Ultra Lot#76H10_>91) 432, 720, 1200, 2000 mg/1 100% 929.5 mg/1 (790.9-1092.5) 1077.7 mg/1 (897.0-1258.4)
1 D-439
US00001620
CUMULATIVE DATA SUMMARY NPDES Permit #: N/A Oceana - ODU B-3
Date. 5. _- of Iny Testr-J';"
2/12/97 >100 2/28/97 Invalid 3/12/97 >100 3/26/97 >100 9/30/97 65 11/3/97 45.0 11/18/97 75.2 11/25/97 69.1
-, Ve"rtebrate>;i-->t'::-;fe Invertebrate^ - .. Vertebrate NOEC % - - NOEC % -
>100
' >100
>100
>100
.
>100
>100
>100
>100
S upplem ent
D-440
US00001621
COA-STrVJ- BIOANALYSTS. INC. NPDES STATIC ACUTE/SALTWATER
(Form A M B)
* EMImnt
i.O.
C-) C-7 1-1 6.25 1 7 2-1 12.5 7-2 3-1 25 3-2 4.1 50 4-2 5-1 100 5-2 ObsTver
` Ture:
Day 1 tiv i' (c to to to (C tc
11 to (c O 2-
<f& tw o
0 rr2 9 tiv
1c
/O
!0
s
/c
? ?
10
...
>o H P
o S *2) nSo
find % *w d
io
/
K
Sr^-ier Mradowis bahia
..J Source
Hatch Dit'Time:_
ii h M i
CSI
Acclirmnon water HWMASW E T O thcr_____________S r t v t f . Q O .
AcriirmtionTirrp. (rangaj:_____ 1
^ ---------
Feedrg trier to r r Artonro , 3 X / d O ther______________________
FWeim during t u t: Not fad D
Artomo / A / d Dthec___
0 r * r r r ; 300 n4 0
500 n4 Q U J C O r r f O O lia r ,
__rrt
TortwhTM: 2OOn4 0
250rrf
Initial no. orwwfm par rrrficatt: I T)
! SOOrrf
i
OtMc_
j Pto. T W & c tW .-----3 s --------
rd
ftotoptnod 161:00
Qthor
. Rwwimmriion andata "Q- S ~ (
SET UP: O it. I I I 7 2 . `j 1 9 1 _ r>rra Wtw Addtd_
(7 /0
Tine Aninell Added / " } 1 - 0 .
Sot Up By f ^ P j P f \ ___
INSTRUMENTATION USED:
Sam Thefrnxnter Marfcar. f i t I
D 1YSI D.O. Melar (SNE80144751
3 . CP OiyronM pH Mette (09500563) Sartori RI SOP Wane (40020093) U YSI SOT 33 Melar (H80) 6797)
0 YSI 0.0. Md#21961A02S329)
C<*TM"9 24 0 1* M*1* (3 6 2 6 8 ) $P P o r ta i Metar 1852360) 0 Spartan ATC Rafraotormter (A366)
C
T1 E2
M p3
4
S
C
2 pH
3
4
5
0.0.
(o rO)
c
1 2
3
4
5
sc
A1
L
2
N3
4
5 Radiata f
liitia li
DayO 2 0 .1 2 *1 D . 0 jfc, *P o 3 Qs> % P fl- I *3 5 "5 B ' O
S ii S .O T 3 03 7 -?
7 -T 7 .7 77
77 7 -^ Po pa 3 i>
O -t P1
7 ^3
Day! p c i.2 / A io
P i3 P .C
7 *1 2 7 ?? 7 .7 ? <1 0 7 < c ,C ?
S iS 7-1 7 .e 79 7? 7S 7 .^ PO PA, ac? P Q - >\ p re >
Oay 2 J O .O 70. 0 20 1 zo 1 n .9 lc . U
7 .r ? 7.5 ^ 3 - S-c1 .1 ? 7 - c/ / 7. 7 A7 7.
Z . _ 7 .^ -
7c 2
- 2 .0 7o
9 O _______ zo
_ i J ________ a
NOTES:
?ivh ~7.\ Q-t ><>ec ~:n l ^ f r
s
Quality A i w v a c
A t QuaSty A m v o offiev fer this mst I cartify tfl no tignfict " t ^ v i t iom from m p t a d pretoed eeeirrtd during reutina impactioni ( Ilm otherwise noted bake*) and that the data ocneatatr tefleet the tast a* it w n larfuoned by CB.
Coffrmntt
---
D-441
TEST t D Z F d f r H 1*5%
US00001622
RIMMED SPEARMAN-KARBER METHOD. VERSION 1.
DATE: 11/25/97 TOXICANT : B3 SPECIES: M BAHIA
RAW DATA:. -------
Concentration (%)
.00 6.25 12.50 25.00 50.00 100.00
TEST NUMBER: J]
DURATION:
Number Exposed
20 20 20 20 20 > 20
Mortalities
0 2 1 1 1
20
SPEARMAN-KARBER TRIM:
6.25%
SPEARMAN-KARBER ESTIMATES:
LC50:
69.10 95% CONFIDENCE LIMITS
ARE NOT RELIABLE.
48 H
NOTE: S S S S E i T S S 1
__________
D-442
US00001623
coIASTAL BIOANALYSTS. INC. SPOTS STATIC ACliTE'S ALTWATER
(F o rm ACV4R)
% Eifluvrit
r.o.
Contrai
6.2S 12.5 2$ 50 100
C-l C-2 1-1 12 2-1 2-2 3-1 32 4.1 4-2 5-1 5-2 Obwfvef: J ut* :
0*y 1 t liw 1O (0 0 10
10
(0
0 (0 /D 10 t0 to ?e>
I 'l l "o
_
0>y 2 t Irvt
firm i* S tm v il
; r ( tt 0
1o
it \ O
c l ^ i t-l-5
i0 i^ i < i c>
1o u 1O o
t 'j iL
SO
"
, CO
S o r e . ____ Qn nodon v a r ie g a t i ___________ ____ Sowrt:
CBI
1 IHatch Oatt/Tin:^__________ I ' i
9 .Z -
Acclimation w ater HWM ASW
Other-----------
Acclimation Tamo (rnget_________ S 1 --------
___ Salinity: 7 -
_2 o _ S ____
Faadng pnor to telt: A rtm a . 7 A
/ <* Other---------------------------------------
E3fra n g drrng tear Not let)
A'' " *------------- 1*
0th*r ------------------------
C W t o . : 300n<
SOOn) 0
lOOOn)
OtS*:--------!------- <*
Teat J i m : 2 n) *m
250 ni
500 ni
Other.---------------- <*
Iretiet no. argentana per replicate:------ _ L Q ---------- ^ replicete*:--
-
Photopenort I6 lj 0 0 . Other - Ramtamatien te n d e te na.--------------
USET UP: Data
/ 7 l 9 ~ 7 Fare Water Adcfcd---- -------------------------
Time'Aiemnlj A d d e d / 0 1 < ______ S atU pB r P O / <'0 -
INSTRUMENTATION USEO:
A l___Stem Thenmrmttf H vrrtrc.
Y 3 D.O. Mrter f I (SNE8014475) S . C P D i g a e ^ Meter (09500563)
Sartorius RI 60P Balance (40020093) Y 3 SOT 33 Mater (HS016792)
,,_
E 3 YSJ 0.0. M ettr 1 2 ISW1A026329) Coming 240 tH M at (SW268)
SP PottatW Meter (852360) Q Scanan ATC Refrectormtei (A366)
Other
C
1T I E
2 M P3
4
5
C
1 2
3
4
5
c
1 20.0.
3
4
5
Sc
A1
L I
2
N3
l^qj 4
5 catt 1
rtitft:
j2 o .fc>
0.0 Ai 5^.0 ?77 X .T 3 1X 7 5 .1 C
i- it 7.9 n \ n3 it
7.*i
-7 .? zo Z-D
T -b 'V
)
^ 2 6 .0
1
3 -C -o Jo O
?.9
i<i7
-1rl - *T
P6-I 7 . to
2< . c o
7 .9 ?
;c .o
7-9f 177
1-*Y
n .l -7 -4
7 .-7 9 7 .9 7 T 05 -- ^
7. 7
7-1
7 .J
_ 2 J ____ ^ 7 . <S
7. i
7 -9
7.
20
C20 J2 -0
T io 7C
Zo
P O to
7* _ 7 ' P ... i
_ i i > _____
NOTES
5 pflOt'g l
t rfcTt; ce i
UfllvCrAAp_____________ _
k -g . 7 Y f t/ ,7>(?T)C .Vt
Qtaliry A ssavc: At QwGty A if u n ro office for tfct t*rt I certify thX m aignitont ifceiationa from accepted pretoqJ occured Aairqipetipeimpectieiat (tinleaa othanm a n o ted b aker) and that the data aeneatatr reflect the tati *s it m s ptrfonmd by C8L
CorfTmi_
D-443
TEST I D.: CTM n / S~<P
US00001624
COASTAL BIOANALYSTS .NC. EFFLUENT/DILUTION WATER DATA F- -- >
EFFLUENT .n
\
i
t e s t Tn r f / L A D-444
t gJL
US00001625
Toxicity Test Sample Chain of Custody (P lease complete all information)
i- ,.,,- n \ d D gtnXvvOyl U i -v erS rtH . - . J , K y y f c l V . V A 2 3 V l -----------------
County. NPDEESS##._. _______ _____________ --
, Sample'collected by (print& sign)_i\
Pipe/Outfall/Location Instream Waste Cone
ZgL
- A ffili,inn
Tvpe of sample ^ .,,((GGrraabb))::
DDatea----l e T i m e T O Q j ^
______ (Composite): From Date
_ T im e.
To Date
___ T im e
\* k
Subsampies comprising composite:
Mnmhi*r --
Frequency of collection--
`Temperature
of
sample
in
sample ~collection
dev
ice-----'--O-
'
Q
s ~
0
'
C
>
Volume.
Final temperature of effluent at sample collection point-------- :--
`Is sample collection device ch illed ?_fi> Is sample packed on ice for s h ip m e n t? _ ^ i_ It is required that all samples remarrHt 0 - 4C during collection period and shipment Ifor
data to be accepted by the appropriate Regulatory Agency (Do not freeze!)^
Is the sample chlorinated?----- -- dechlorinated?-------- If so, h ow .__ .
--
Permit with interim chlorine limit?-------------- If yes " lirrilt C111^ ) -
Field r H <L & Q
Field Total Residual Chlorine
'
Comments/Sample description._ 2 l
7upe of cs.Cs) ,0 be pe,fanned (Specify organisms)
`
Method of s h i p m e n t . ------------------Print & Sign Names
/_,
1 . Relinquished Received b
y
T
Relinquished by_
Received by ^ i Q O C _C >
Tta<;
i L/ai^ rmI f t T e
--- `
'(Date
Time.
-jS i/3 8 6
l
D a t e _ U _ 2 2 5 z l l_ Time. / LM Q
FOR REED LAB USE ONLY
jR A # _ 3 ^ m i2
Arrival Temperature.
Color------------------- ---- pH----------- DO (mg/L).
___ Odor. ____ Conductivity (pm hos/cm )
Salinity (ppt).
_ TRC (mg/L)__:___ _ Method.
On ice?
D-445
US00001626