Document YrkYQkdZBDzv2O6Q8796yX71k

O PCB PRODUCTS ARE NOT A SINGLE ENTITY, BUT COMPLEX MULTI COMPONENT MIXTURES O PCB RESIDUES FOUND IN WILD LIFE ARE DOMINANTLY PENTA-, HEXA-, HEPTA-, AND OCTACHLORO BIPHENYLS O PCB RESIDUE ARE MOST SIMILAR TO AROCLOR 1254 AND AROCLOR 1260 PRODUCTS PRIMARY BACTERIAL DEGRADATION STUDIES o SEMI-CONTINUOUS ACTIVATED SLUDGE DEGRADATION RESIDUE ACCUMULATION STUDIES O FISH - CATFISH AND BLUEGILLS O BIRDS - WHITE LEGHORN CHICKENS MAMMALS - ALBINO RATS AND BEAGLE DOGS v. _ y a OM CCC235 ASSESSMENT OF THE PERSISTENCE' OF POLYCHLORINATED BIPHENYLS IN BIOLOGICAL SYSTEMS AOH C0C24C tk ) u t.. \ Semi-Continuous Activated Sludge Test Unit 'V V ADM 0002*2 MECHANICAL C Y C LE SYNTHETIC SEWAGE AND AROCLOR (lin g ) ADDED ACM 0C02A3 Sem i--Cnlinuous Activated Sludge Degradation of Polychlorinated Biphenyls ft Aroclor 1254 ADM COC2AA I Electron Capture Gas Chromatogram Residue - 1hr. After Addition of Aroclor 1242 L 0 f >1 v ..1 Electron Capture Gas Chromatogram: Residue- 7 2 hr. A fter Addition of Aroclor 1242 : 4 6 8 10 \ Elution Time (minutes) 12 0C045 : O DEGRADATION STUDIES" CONCLUS IONS RATE OF PRIMARY DEGRADATION INCREASES AS THE DEGREE OF CHLORINATION OF THE AROCLOR PRODUCT DECREASES BIPHENYL>AROCLOR 1221>MCS 1043 >AROCLOR 1016>AR0CL0R I242>AR0CL0R 1254 O BIPHENYL, MONO-, DI-, TRI-, AND TETRACHLORO BIPHENYL HOMOLOGS UNDERGO PRIMARY BACTERIAL DEGRADATION ACM 0002^6 o ,/ aroclor residue studies t ACM OCQZHl WHITE LEGHORN CHICKEN STUDIES PRODUCTS STUDIED NUMBER ) FEMALE OF ) CHICKENS) MALE NUMBER) OF ) SAMPLES) MUSCLE L IV E R FAT EGOS CHICKS NUMBER) OF ) SAMPLES) ANALYZED) MUSCLE LIVER FAT EGGS CHICKS .9 0 DAY ORAL AROCLOR 1242 AROCLOR 1254 AROCLOR 1260 AROCLOR 200 80 40 16 40 40 40 *250 30 8 8 8 *6 0 10 14 5 12 4 18 5 25 8 . .11 10 TOTAL SAMPLES COLLECTED TOTAL ANALYZED 400 80 121 32 ORAL EXPOSURE C AR R iEO O U TBY INDUSTRIAL BIO-TEST LABORATORIES, INC. NORTHBROOK. ILLIN O IS ADM CCC2<rS RESIDUE STUDY OF AROCLOR I2A2 ORAL EXPOSURE LEVEL IN WHITE LEGHORN CHICKENS 90 DAY ORAL HOMOLOG DISTRIBUTION PPM PCBs 1234 567 1 10 100 tTHEORETICAL RESIDUE 126 1260 12602 - (2 ) (3 ) ( 4 ) ( 5 ) 6 R ESID U E, 12 WEEK EXPOSURE RESIDUE, 30 DAY RECOVERY 14 136 1312 - (3 ) 14) (5 ) 6 ' - 9 77 749 - . (3 ) (4 ) (5 ) (6 ) - t I F TOTAL PCB CONSUMED HERE RETAINED AVERAGE PPM IN L IP ID > ALL TISSUES ( )ELECTRON CAPTURE PEAKS GREATER THAN 5* OF TOTAL ADH CC0249 ADM 00025C RESIDUE STUDY OF AROCLOR I25A IN WHITE LEGHORN CHICKENS 90 DAY ORAL. HOMOLOG DISTRIBUTION PPM PCs 1234 56 78 9 ORAL EXPOSURE LEVEL 1 10 100 10 +THEORETICAL RESIDUE 126 1260 12602 3 (4) (5) (6) 7 - - RESIDUE, 12 MEEK EXPOSURE RESIDUE, 30 DAY RECOVERY 38 362 3506 17 164 1580 - 4 (5 ) (6) 7 - - - 5 (6 ) 7 _ _ _ t I F TOTAL PCB CONSUMED WERE RETAINED AVERAGE PPM IN L IP ID ALL TISSUES ( ) ELECTRON CAPTURE PEAKS GREATER THAN 5 * OF TOTAL l'1. I' RESIDUE STUDY OF AROCLOR 1260 IN WHITE LEGHORN CHICKENS 90 DAY ORAL HOMOLOG DISTRIBUTION PPM PCBs 1 23456 7 89 ORAL EXPOSURE LEVEL 1 10 100 +THEORETICAL RESIDUE 126 1260 12602 - (5) (6) (7) 8 10 *RESIOUE> 12 WEEK EXPOSURE 65 607 5909 - - - 5 (6) (7) 8 - ' - * RESIDUE, 30 DAY RECOVERY 26 232 2363 - - - 5 (6) (7) 8 + IF TOTAL PCB CONSUMED WERE RETAINED * AVERAGE PPM IN L IP ID - ALL T IS S U E S ' ( ) ELECTRON CAPTURE PEAKS GREATER THAN St OF TOTAL ACH 0C0251 rv \ O ACh CCC25 n j RESIDUE STUDY OF AROCLOR 1242 IN ALBINO RATS TWO YEAR CHRONIC ORAL EXPOSURE ORAL EXPOSURE LEVEL PPM PCBs 10 100 HOMOLOG DISTRIBUTION 1 2 3. 4 5 6 7 8 tTHEORETICAL RESIDUE 803 8037 80373 - (2 ) (3 ) (4 ) (5 ) 6 - RESIDUE, 3 MONTH EXPOSURE 6 23' 90 - (3) (4) (5) 6 - . - RESIDUE, 12 MONTH 9 37 155 EXPOSURE (3) (4 ) (5 ) 6 - RESIDUE, 24 MONTH 12 53 240 - -- (3 ) (4 ) ( 5 ) 6 EXPOSURE 9 10 - -- -- -- t I F TOTAL PCB CONSUMED WERE RETAINED . AVERAGE PPM IN L IP ID - ALL TISSUES ( ) ELECTRON CAPTURE PEAKS GREATER THAN 5X OF TOTAL ACM 000253 RESIDUE SlUUY ui" a\ k U LLU I\ ICJH. im /-\u.uinu u ORAL EXPOSURE LEVEL TWO YEAR CHRONIC ORAL EXPOSURE HOMOLOG DISTRIBUTION PPM PCBs 1 10 100 1 2 3 4 5 6 7 8 .3 .10 ^THEORETICAL RESIDUE 803 8037 80373 - 3 (4) (5) (6) 7 - RESIDUE, 3 MONTH 13 94 679 EXPOSURE - 4 (5) (6) 7 - RESIDUE, 12 MONTH 24 189 1471 EXPOSURE - - 4 (5) (6) 7 RESIDUE, 24 MONTH 42 355 3038 EXPOSURE - - 4 (5) (6) 7 - t I F TOTAL PCB CONSUMED WERE RETAIND AVERAGE PPM in l i p i d - Al l TISSUES ( ELECTRON CAPTURE PEAKS GREATER THAN 5* OF TOTAL 00025^ oa*: f "\ s RESIDUE STUDY OF AROCLOR 12,60 IN ALBINO RATS TWO YEAR CHRONIC ORAL EXPOSURE ORAL EXPOSURE LEVEL PPM PCDs 1 10 100 HOMOLOG DISTRIBUTION 1 2 3 4 5 6 7 8 9 10 tTHEORETICAL RESIDUE 803 8037 80373 - - (5) (6) (7) 8 - "RESID U E, 3 MONTH 18 134 970 EXPOSURE - (5 ) (6) (7) 8 "RESID U E, 12 MONTH 35 270 2099 EXPOSURE - (5) (6) (7) 8 "R ESID U E, 24 MONTH 59 507 4338 EXPOSURE - (5 ) (6) (7) 8 t I F TOTAL PCB CONSUMED WERE RETAINED "AVERAGE PPM IN L IP ID - ALL TISSUES ( ) ELECTRON CAPTURE PEAKS GREATER THAN 5X OF TOTAL ADM 0C0255 1000 90 Day Subacute Oral Exposure Study of Aroclor 1221, Aroclor 1242, Aroclor 1254, and Aroclor 1260 in Albino Rats at the 100 ppm Exposure Level < ji l 500 BEAGLE DOG STUDIES I 2 YEAR CHRONIC ORAL 90 DAY SUBACUTE ORAL AROCLOR 1242 1 PRODUCTS STUDIED AROCLOR 1254 . AROCLOR 1260 . .AROCLOR 1221 I NUMBER ) FEMALE 40 OF DOGS) MALE 40 1 NUMBER ) MUSCLE 54 OF ) LIV ER 54 SAMPLES) FAT . 54 I *= TOTAL SAMPLES COLLECTED Z42 14 14 7 7 :7 '-- 21 HU" | ER | MUSCLE SAMPLES) ANALYZED) Hl 42 40 43 7 7 7 TOTAL ANALYZED 125 21 ; il ORAL EXPOSURE CARRIED OUT BY INDUSTRIAL BIO-TEST LABORATORIES, INC. NORTHBROOK, IL L IN O IS I ADM ) RESIDUE STUDY OF AROCLOR I2A2 IN B EA G LE DOGS TWO YEAR CHRONIC ORAL EXPOSURE ORAL EXPOSURE LEVEL PPM PCBs 1 10 TOO HOMOLOG DISTRIBUTION 1 2 3 4 5 6 7 8 9 10 tTHEORETICAL RESIDUE ` RESIDUE, 2 YEAR EXPOSURE 519 5186 51865 2 6 12 - (2) (3) (4 ) (5) 6 - - ( 3 ) ( 4 ) 5 6 ( 7 ) ( 8 ) - ^RESIDUE, 30 DAY RECOVERY 1 4 10 - - - 5 (6 ) (7 ) (B ) - - ` RESIDUE, 60 DAY RECOVERY 0 .8 3 6- - - 5 6 (7 ) (8) - - t I F TOTAL PCB CONSUMED HERE RETAINED ` AVERAGE PPM IN L IP ID - ALL TISSUES ( ELECTRON CAPTURE PEAKS GREATER THAN 5X OF TOTAL 957000 WOV V RESIDUE STUDY OF AROCLOR 12,54 IN B EA G LE DOGS ORAL EXPOSURE LEVEL -(-THEORETICAL RESIDUE TWO YEAR CHRONIC ORAL EXPOSURE PPM P C B 5 1 10 100 1* 2 HOMOLOG DISTRIBUTION 345678 9 519 5186 51865 - 3 (4) (5) (6) 7 - - RESIDUE. 2 YEAR EXPOSURE 7 30 132 - - - (5) (6 ) (7) 8 - RESIDUE. 30 DAY RECOVERY RESIDUE, 60 DAY RECOVERY 5 24 109 4 20 88 - - - 5 ( 6 ) (7) 8 - - - 5 (6) (7) (a) - t I F TOTAL PCB CONSUMED WERE RETAINED AVERAGE PPM IN L IP ID - ALL TISSUES ( ) ELECTRON CAPTURE PEAKS GREATER THAN S t OF TOTAL 10 C0C259 I RESIDUE STUDY OF AROCLOR 1260 IN BEA G LE DOGS TWO YEAR CHRONIC ORAL EXPOSURE ORAL EXPOSURE LEVEL tTHEORETICAL RESIDUE PPM PCBs 1 10 100 HOMOLOG DISTRIBUTION 1 2 3 4 5 6 7 8 9 10 519 5186 51865 - - - (5) (6) (7) 8 - 'R E S ID U E , 2 YEAR EXPOSURE 8 63 388 -. (6) 7 (8) - - 'R E S ID U E , 30 DAY RECOVERY 7 60 363 - - - - (6) (7) (8) - - 'R E S ID U E , 60 DAY RECOVERY 7 55 337 --- 6 (7) (8) - - t I F TOTAL PCB CONSUMED WERE RETAINED AVERAGE PPM IN L IP ID - ALL TISSUES ( ) ELECTRON CAPTURE PEAKS GREATER THAN 5X OF TOTAL ADM C0C26C r\ G "1 ) Two Year Chronic Orol Exposure in Beagle Cogs (a t The Ippm Feeding Level) ADM 000261 n* WHITE LEGHORN CHICKENS ALBINO RATS BEAGLE DOGS EXPOSURE LEVEL (PPM) EXPOSURE PERIOD CONCENTRATION FACTOR* 1 .0 (FEED) 1 .0 (FEED) 1.0 (FEED) 12 WEEKS 2 YEARS 2 YEARS ` 20 9 2 CONCENTRATION FACTORS DO NOT TAKE INTO ACCOUNT EXPOSURE PERIODS ADM 000262 CONCLUSIONS RESIDUE BUILDUP STUDIES O' FOR ALL. PRODUCTS THE PCB. RESIDUE LEVEL IN CREASED AS THE EXPOSURE LEVEL AND PERIOD INCREASED Q RELATIVE TO AROCLOR 12 60 , THE HIGHEST CHLORINATED PRODUCT STUDIED, THE PCS RESIDUE LEVEL DECREASED EXPONENTIALLY AS AS THE HEIGHT X CHLORINE OF THE PRODUCT DECREASED O RELATIVE LEVELS OF PC8 RESIDUES FOUND WERE FISH>CHICKENS>RATS>DOGS O RELATIVE PCS' RESIDUE LEVELS IN TISSUES WERE - CHICKENS RATS DOGS FAT = MUSCLE>LIVER LIVER>FAT MUSCLE>KIDNEY LIVER>FAT = MUSCLE O FOR ALL PRODUCTS THE DOGS AND RATS EXCRETED AND/OR METABOLIZED 93 TO 992 OF THE TOTAL AROCLOR ORALLY INGESTED O CHICKENS EXCRETED AND/OR META30LIZED PRODUCTS INJESTED ORALLY AS FOLLOWS - 90S OF AROCLOR 1242 70S OF AROCLOR 1254 SOS OF AROCLOR 1260 \ I I. Q i; ! II . ADM 0C0263 [ ! CONCLUSIONS RESIDUE FALL-OFF O DOGS AND CHICKENS CONTINUED TO EXCRETE AND/OR METABOLIZE PCB RESIDUES, INCLUDING PENTA AND HEXA HOMOLOGS, RETAINED WHEN PLACED ON PCB FREE RECOVERY DIETS DOGS> CHICKENS G RELATIVE RESIDUE FALL-OFF RATES SHOWED DOGS: AROCLOR 1242>AROCLOR 1254 >AROCLOR 1260 CHICKENS: AROCLOR 1242>AR0CL0R 1254>AR0CL0R 1260 ACM C0026A CONCLUSIONS j R E S I D U E STUDIES .. ALTERATIONS OF ARCLOR HOMOLOG DISTRIBUTION O ALTERATION OF THE HOMOLOG DISTRIBUTION OF ALL PROOUCTS WAS OBSERVED IN 'A LL STUDIES EXCEPT .FISH O LOWER CHLORINATED HOMOLOGS PRESENT IN ALL PRODUCTS WERE PREFERENTIALLY EXCRETED AND/OR METABOLIZED j 1 I iLt ERATION OF THE HOMOLOG DISTRIBUTION . INCREASED AS THE WEIGHT X CHLORINE OF THE PRODUCTS DECREASED (INCLUDING PENTA AND HEXA HOMOLOGS) ' I AROCL 03 1221 AROCLOR 1242>AR0CL0R' 1254 >AR0CL0R 1260 , DOGS>RATS>CHICKENS>FISH \ I ADM C0C265