Document YrQGX43EeevVr6mQ8Xy96aEb8

AR226- 0o4H ACUTE TOXICITY TO THE SALTWATER MYSID LE-F TESTSUBSTANCE Identity: PFeCr-f9lu5o.ro(o1c-tOacnteasnuelsounlaftoen;icmaaciyd,al1s,o1,b2e.2r.e3f,e3r,r4e,d4,t5o,5a,s6,P6,F7O,7S.8o.r8.8heptadecafluoro-, potassium salt, CAS # 2795.35-3) fRreommar3kMsl:ot nuTmhbeetres2t17s.ubsStaamnpcleeiws aaswhsittoerepdowudnedre.r Sample ambient was taken conditions pNrMioRr taontdesetilnegm.enPturailtyandaeltyesremsinteedchtnoiqbuees9.0.49% by LC/MS, 'H-HMR, 'F- METHOD Method: OPPTS 850.1035 Type: Static acute GLP: Yes YSpeeacriecosm:pMlyestieddo:psSitsudbyahcioampleted 1999. Report completed 2000 ASnuaplpyltiiecra:lImno-nhiotuosreincugl:tuPreFs,OSWilmdeiaiseuIrnetedrnaatti0o,n4al8,, $Lt6d-.h,ouErass.ton, MD Exposure period: 96-hours Santaaltyissitsi,camlomveitnhgoadvse:raLgCesmveatlhuoeds coarlbciunloatmeida,l wprhoebnabiploistsyibwliet,h by probit non-linear interpolation using the computer Test fish age: < 24-hours old software of C.E. Stephan Pretreatment: None Test conditions Dilution water: 0.45,m filtered, Natural seawater diluted to 207%, with well water, Diimlmuetdiioantwealtyerprcehceemdiisntgryth(edutersitn)g: the 4-week period Salinity: 20 (20-20), pH: Toc 8<21(.80.m1g-l8L2) S8.t2ocmkg/aLndantdesmtisxoeldutfoiron~2p2rehpoaurartsiporni:or tPoriusmea.ryAsfttoecrkmpirxienpga,red at p{0ripmraerpyarsetotchkesfooluurtiaodndiwtaiosnaplrotpeosrttcioonnaclelnytrdaitliuotneds.wiAthl dtielsuttisoonluwtaiotnesr. aCopnpceeanrterdactlieoanrsanddoscionlogrlreastse.: Once Stability of the test chemical solutions: Extremely stable 20930 `Eaxppporosxuirmaetveleyss1e0ls0:0m2LLopfotleysettshoylluetnieona;qwuaatreira dceopnttahinaipnpgroximately 66cm Number of replicates: two Number of mysids per replicate: ten Number Feeding: of concentrations: five plus a Live brine shrimp nauplii daily negative control Water chemistry during the study: Dissolved oxygen range (0 -- 96 hours). 687.4 mg/L 6.8-7.3mglL (control exposure) (5.4 mg/L exposure) PH range (0 - 96 hours) 8.1-8.2 8.1-8.2 (control exposure) (5.4 mg/L exposure) Test temperature range (0 -- 96 hours) 24.2-25.4C (control exposure) 23.8-245C (5.4 mg/L exposure) Method of calculating arithmetic mean mean measured concentrations: RESOLYS OOOOO OOOOOo MNoemaisnuarledcocnocnecnetnrtartaitoinosn:s: Bk<Lco0nQt,rol0,.517.,1,1.11.,8,1.39.,0,3.40.,9,5.84.2mmggl/LL Element value: 2448--hhoouurr LLCCeso == >> 55..44 mmgg//LL (CC.II.nnootccaallccuullaabbllee)) 9762--hhoouurr LLCCssoo == 34..64 ((33..06-64..62)) mmgg//LL All element values based on mean measured concentrations Statistical for 24 and Evaluation 48-hours of of Mortality: exposure due LCso to the vlaalcukeosncoaunldadneoqtuabtee.calculated concentration-response pattern. mortality at 72 and 96 hours. The probit method was used to evaluate WAinladliyfteicIanlteMrenatthioodnaolloLtgd.y:uAsinnaglyhsiegsh opfertefsotrsmoalnucteiolnisquwidercehrpoemraftoorgmerdapahty cwiotnhcemnatrsastisopnecoifrtohmeettreisct sdeutbescttainocne(inHPtLhCe/tMeSs)t.soWluhtieonns,dettheermsianmiengatnhde `Nmoosattptermopmtinweanst pmeaadkerleosqpuoannsteiffyoronpetrhfeluboarsoiosctoafniendsiuvlifdounalatieswomaesriucsed. Tcohmepomneeanntsp.ercTehnet LreOcQov(elriymitofofmaqturainxtiftoarttiiofinc)atwiaonss 0a.n1a1l5yzmegdlLcoinncuthrirsensttludyy. during sample analysis was 97.4. Samples collected at test initiation had 00931 measured values from 52.4 to 70.7% of nominal. Measured values for samples taken at 48-hours ranged from 43.5 to 71.0% of nominal. Measured values for samples taken at 96-hours ranged from 35.5 to 71.1% of nominal. `Summary of analytical chemistry data: Ee a 2 [mec | matos |wea | | Co Trem oe|] [eo [ermue|m0o|nwos| [en [oo [wrmaser| msoa|no an| Lee [omsmase|sm0o|nwon| Biological observations after 96-hours: Mysids in the negative control, and the 0.57 and groups appeared 1.1 mg/L (mean measured normal and healthy during concentrations) the test, treatment i=e] [oe [om[o-| `Cumulative percent mortality: hoscowal 0 | 0| 0| &| LoeToTwTw| w] Mortality of controls: None 000932 Conclusions 0 The was dpeottearsmsiinuemdpetorfbleuo3r.o6ocmtga/nLeswuiltfhonaat9e59%6-choonufridLeCncsefionrtseravlatlwaotfe3r.m0y--si4d.s6 mg/L. The 96-hour no mortality and NOEC concentration was 1.1 mgiL. Submitter: 3M Company, Paul, Minnesota, 55133 Environmental Laboratory, P.O. Box 33331, St. bavaquaAtRY 000000000000OO Reliability: Kiimisch ranking = 1 REFERENCES This study was conducted at Wildife Intemational Ltd., Easton, MD at the request of the 3M Company. ower 00000000000 Last changed: 5/3/00 00933