Document YpLG3wVBJjV9j1z5aoxYjBOV

FOR DU PONT USE ONLY AR226-2900 E. I. du Pont de Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine Elkton Road, Newark, Delaware 19711 HASKELL LABORATORY REPORT NO. 369-81 MR N O ^ J Material Tested Haskell No. 14,008 Other Codes Study Initiated/Completed 6/1/81 - 6/5/81 rMaterial Submitted by Polymer Products Department Experimental Station 96-HOUR LC50** TO FATHEAD MINNOWS Procedure: The test material, after being pulverized with mortar and pestle, was introduced into all-glass exposure vessels and diluted with laboratory supply water to yield the desired test concentrations In 15 liter final volumes. All concentrations iere aerated for mixing purposes only prior to adding test animals. An identical vessel, containing only laboratory supply water, was designated as the control. .Ten fathead minnows (Pimephales promelas) with a 2.7 cm mean standard length and 0.27 g mean wet weight were randomly assigned to each test vessel. Fish were not fed for 48 hours prior to nor during the exposure, the test solutions were not aerated during the exposure period and temperature was maintained at 22"C. Photoperiod was maintained at 16 hours light:8 hours dark. Mortality counts and observations were made every 24 hours during the 96-hour exposure period. Dead fish were removed as they were discovered. pH was measured at the beginning and termination of the study, dissolved oxygen was measured at the beginning, 48 and 96 hours in the control, low, medium and high test concentrations. Total alkalinity, hardness (EDTA) and conductivity were measured at the beginning of the test in the control vessel. Company Sanitized. Does not contain T S C A CBS Results; Percent mortalities for fathead minnows exposed to H-14,008 are presented In Table I. The chemical and physical parameters measured during the test are reported In Table II* S u m m a y i B ^ H H ^ H H I I ^ ^ H H ^ I I i ^ f t x h i b i t e d low acute toxicity to fathead minnows under static, unaeracta test conditions during a 96-hour exposure* The 96-hour LC50 is estimated to be greater than 3,200 ppm but less than. 4,200 ppm of the test material in laboratory supply water. * * * LC50 - The concentration which is lethal to 502 of a test population during the specified time period. RRD;FXP:CDL:jrg Report No. 369-81 Report by Approved by Study Director: JO, Richard R. Driscoll Technician Wf. Frank X. Phillips Research Biologist Approved by: _________ ______________ Carol D. Litchfd Chief, Aquatic and Microbial Biology Section - 2Company Sanitized. Does not contain T S C CBS TABLE I RESULTS OF A 96-HOUR ACUTE TOXICITY TEST WITH FATHEAD MINNOWS EXPOSED TO H-14,008' Nominal Test Concentrations ppm (w/v) 7,500 5,600 4,200 3,200 2,400 1,800 1,350 1,000 Control __________ Observed Mortality (g) 24 Hr. 48 Hr. 72 Hr. 100 100 100 96 Hr 100 100 100 100 100 100 100 100 100 0 10 30 40 00 00 0000 000 0 000 0 0 .0 0 0 - 3- Company Sanitized. Does not contain T S C A CBI TABLE II RESULTS OF PHYSICAL AND CHEMICAL PARAMETERS MEASURED DURING A 96-HOUR ACUjSE TOXICITY. TEST WITH FATHEAD MINNOWS EXPOSED TO H-14,0081 Nominal Test Concentrations 7,500 ppm (High) 3,200 ppm (Medium) 1,000 ppm (Low) Control Dissolved Oxygen (ppm) 0 Hr. 24 Hr. 48 Hr. 96 Hr. . 8.0 8.0* - 8.0 8.0 8.4 - --- 6.4 6.4 6.6 6.2 4.7 5.7 pH 0 Hr. 24 Hr. 96 Hr. 7.5 7.5* 7.7 7.6 7.3 7.5 7.3 7.1 Total Alkalinity (mg/L as CaC03) ! 0 Hr. 101 EDTA Hardness (mg/L as CaCO^) 0 Hr. 85 Conductivity (umhos) 0 Hr. 190 * Final values obtained at 24 hours because complete mortality had occurred during Che first 24 hours of exposure* 4'Company Sanitized. Does not contain T S C CBE