Document YjzvRXapGNZDok9wQqdGw54mN
SDRTPITST-B6295.8, T-6316.4, T68682, T20711, T7132. 3M MEDICAL DEPARTMENT, CORPORATE TOXICOLOGY
T-6295),`TNi-tleet:hCyolmppearrfalutoirvoeocMtoalneecsuullafronBaimoildogoyeotfhaPneorlfl(uNo-rEo(ocFtOaSnEe,suTl-f6o3n1a6t)e,(NP-FEOtSh,yl acepteartfel(uoFrOoSoAcAta,nTe-s7u0l7f1o)n,amaindde/o(rNP-eErFfOluSoAr,coTc-t6a8n6e8s)u,lPfeornfalmuiodreo(oFctOaSnAe,suTl-f7o1n3a2m)iodfo in
Rats and Guinea Pigs following Oral dosing.
AMENDED Final Report Date: July, 16 2004
Study Numbers. `T-6295.8, T-6316.4, T-6868.2, T-7071.1, T-7132.1 Strategic Toxicology Study Number: DT-15-B
Sponsor: Study Location(s):
Study. Director: Study Toxicologist: In-Life Start Date In-Life End Date In-Life Start Date In-Life End Date In-LifeStart Date In-LifeEnd Date
3M Specialty Chemicals Division 3M Center, Building 236 Saint Paul MN 55133-3220
1. 3M Strategic Altemative Toxicology Laboratory 3M Center, Building 270-SB-181 Saint Paul, MN 55133-3220
2. UniversityofMinnesota, Duluth Dept. ofBiochemistry and Molecular Biology School of Medicine 10 University Drive Duluth, MN 55812-2496
Andrew M. Seacat Ph.D., DABT Toxicology Specialist 3M Medical Dept. Corporate Toxicology and Regulatory Services
Deanna Luebker M.S Senior Toxicologist 3M Medical Dept.
Protocol: Protocol: Amendment #1: Amendment #1: Amendment #2: Amendment #2:
117161998 112011998 03/01/1999 03/05/1999 02/19/2001 02/23/2001
bSTRIPSBT0295, T-63164, T6065 2, 170711, F-71321
Ihe purpose lor tus amendment was 1 change the conclusion drawn in Ue sui Uist Lin Xu and Drag Anders wrote in Appendix GE (page 59) which read:
The
onginal statement. "Thesedata showed
that
FOSA
was
consistently
identified
as
a
metabolite
of
PEOS, whether PFOS was administered directly or formed asa metabolite, but the
sourceof the amino group is not readily apparent."
Was
changed 10 read. "These data showed
tha
FOSA
was
consistently
denufied
us
a
metabolic
of
PFOS, but that the sourceofthe amino group is not readily apparent."
SIuhpeporrotnedablyftohretdhaetsae cprheasnegnetsedweirnethtihsatsttuhdey.orTghinaatlcocnocnlculsuisoinonwwaassnontotendtiirreecltylycorrect wbietchaiunsethaellpoafrtenhtecaodmmpionuisntde.redThceormefpooruen,dtsh,ebleiskieldyessoPuFrOcSe,ofhtahvee aamsiunlofognraomuipdofogrroup issulqufaonntaimtiadtievemoieevtiydecnocnetainintihnegsecosmtupdoiuesndssugwgoeusltidnbgetthhaetptahreent compound itself. There ptheartflPuFoOroSvcbtaucnkestoulafosnulafmoindaemsiwdee.reTmheetaobnollyiezveiddfeinrsctetfoorPFa OpoSssifbollelomweetdabboylmiestmaobfolPiFsOmS.of 1d0enFvOatSiAve cpaarmeentfrcoommpthoeunPdFOdSosdeogsreougprso.upFsu,rntohtertmhoerpee,rifnlutowroooocutlaonfestuhlrefeonianmdiedpoendent SFtOuSdiAe,s asnuaglgyezsetidnagtpRooscshiebslteecr,ontthaemiconnattrioolnogfrotuhpe lliivveerrssaammpplleess.hTahderaehfiogrhe,baascksgtartoeudn,dthoef conversion of PFOS to PFOSA would have 10 be verified by further studies.
Dr Anders s in agreement with the amended conclusion stated above:
.
'
ET
=d e ely
en
Final Repon Amendment 71 prepared by,
;
Andrew M. Seacat, PhD, DABT Study Director
Date.
oSKTPITSTS6295, 1-6310.4, 1-68682, T7071, T-71321
E. Technical Rochester.
Report:
Liver
Fluorocarbon
Metabolites
-
University
of
Tide: `Summary of Quantitative Analysis of Fluorocarbon Metabolites in Rat Liver Samples
Lin Xuand MW. Anders he paren0t asndi m4ebtaibolsiutemsmoafrtyhoefluthoeraoncaalrybsoinssowfelrievdeestaearnmpilneesdfirnomvrrutssagmpilesavabeyngnLeCo-fMSRuMoSr.ocaTrhbeons resus ar presented below.
ontol atTs,hfiogmroauspsgoifvelinve4r0smamgp/ledsawyerFeaOnSlAyzordal:ly,Thaendfirrstogmrorautps(gGivreonup1610)comngsi/sdteadyofFliOvSerAsAfrom ((TMa5bl6e)1)o.nlyT.he dTahtaeschonocwentthartatPiFoOnSowfpaasrtehnecmoajmoproumentdasboanlitethfiorunmdetianbotlheitleisveirsvoferrastswgeirveemneFaOsSurAe:d iFOsSgAivNe-nglFuOcSurAoAni(deM3w6a)s:idPenFtOifSiendd15FOmSiAn.orm1etianbootleiwco.rtThywothmtettahbeoclointtersowleanrimdaelnsi(fIiReO0n7t42h,e iver of FRiOs0ch7e4r33,44anrdatsIRmOa0i7nt4a5i)nceodnitnaitnheedUsniigvneirfsiictayntofcoRnoccchnersutreironVsiovfarFiOumSAdi.d Anotpashrolwedaentaelcytsaibsloef vers from concentrations ofFOSA.
ron contTrohl astesc,ofndogmrorautps(gGirveonup402)mfggritdsawyasPrFeOsSidorailnlyN,ofrvomera,ts g19i9v3e.n 4G0romugp/k2gc/odnatyaiNn-eEdIlFiOveSrEs sciocmophoolunordasllayn,danddenfirofmerdatmsegtiavboelnit4e0smagr/eksgh/odwany iNn-TEeWbFlOeS2.A oTrhalelyd.tTahsehcoonwctehnattrlaitvieorsnsfrofoemrraetnstgiven sFiOveSn Nco-nEtaFiOnSedEpaalrceonhtoclocmopntoauinndedPsFeOvSer;alFmOeStaAbowlaisesa.lsThfoeumnadjoirhmeeteabsoalimtpelsesw.eTehNe-vEeIrFsOSfAroAm,rPotFsOS. aanndd FFOOSSAAAA-(gMl5u3c6u)ro.niTdeh.e mPiFnOoSr mweatsabtohleitmeasjowrermeetFaObSoAli.cFiOnSliEvearlscofhroolm, Nat-sEtgiFvOeSnENa-lGcUoFboOlSgAl;ucFuOroSnAidae.nd rFtOsSdAidN-nogtluccounroanniddeetweecrteabildeenctoinfcieendtrssatmiionnosrofmeptaarbeonl:itceos.mpoInuncdonstoerstmettoabGorloitueps.1 the Ivers from control
nous rashgievehni4nd0gmrgoukpd(oGyrouPpF3OSwoarsaltlry,eaatenddifnrMoamrrcahs,g1i9v99e.n 1G6r0moeu3pkgciodnatyaiNn-eEdSvFeOrSsEfaolmcochoonotroalllryt, TIohwe ccoonncceennttrraattiioonnss ooff thFOScAhewmeicraelisdaenntdifmieetdaibnolfiitveesrarfrosmrhaotwngiinvTeanbPlFeO3S. . AsInwtihteh lGirveoruspof2,raPtFOgiSveannd mEiInFoOrSmEetaalbcoolhiolt,esV-enEt(iFfOiSeAdA.werPeFOFSO,SAn,dFFOOSSEAaAlo(hMalS,56-)EwUeFOeSiEdenatlicfoiheodlaglmucaujroornimdeet,abaonldiFcsOSaAndNt-he lucuronide. Similar 0 Group 1,control animals showed detectable hepatic concentraotfiFOoSnAs. SDicshceumsesi)o,n:butTdhoesneodtaatllaolwloiwnfceornecncleussiaobnosutabtohuetetxhaectovoeralluomfemtetatabbooslliitce foofrtmhaetilonu.oroFcOarSbAonwsassudied sce cWohantseivsetredisirdoeunttiefoiefdf3orm3atmieotna,bFolOiScAofwaPsFOmSe,tabbuotltihzeedsotuorFceOoSfAthNe-galmuicunroognirdoeu.p is no readily appurcnt
ans umA-hHeIFN-OdSeEetahlycloahtoilongiovfes\r-iEseV1FsOSraanlgcoehoolf,maanjdor-aEnHdFmOinSeAsAmeccoaublldiaersi.se bFyOtShEoaxlicdoahtoiloncooufldthe aNl-cEohLoFlOtSoEthaelccoahorlbgolnuiccuraocniidd.es.GluFcuOrSonAiAdactoiuolndobftehfeorpmareedntby-Eth(eFNO-dSeEcthayllcoahtoilonwoofulNd-EgiIvFeOtShAAobosrerbvyedthe omxoidvaatilonofotfheFcOaSrEbaolxcyomheot,hyolr bgoth.roFofOFSuOAScApoAuldasbgelfyooxrymlaetde.by FthOeSNA-dNe-cgtlhuylcautrioonniodfe mNa-yEIbFeOfSoArmoerdbbyythtehe lcuronidationof FOSA.
Revised page 39