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EE ACUTE TOXICITY TO FISH ARLE 0 1] TEST SUBSTANCE. Identity: Perfluorooctanesulfonate, Lithium salt; may also be referred to a1,s1,P2F,2O,S3,3Li,4s,a4lt,,5,F5C,-69,64,,7,o7r,F8,C8-,984--hXe.pta(d1e-cOacftiaunoreos-u,lflointihicumacsiadl,t, CAS # 29457-72-5) Remarks: Test sample was taken from 3M production lot #1. The test sample is a mixtureofthe test substance in water (approximately 24.5% test substance and 75.5% water). No calculations were madeto adjust for the actual concentration of the test substance in the test sample. METHOD Method: Not noted. Type: Static acute 3 GLP: No Year completed: 1994 Species: Pimephales promelas Supplier: Aquatic Biosystems Inc., Fort Collins, CO Analytical monitoring: pH and DO content Exposure period: 96-hours Statistical methods: LCs values calculated by Trimmed Spearman - Karber. Test fish age: 79 days. Length and weight: Average length = 2.1 + 0.3cm, Average weight = 0.069 +0.03g 3 Loading: 0.69 g fish /L Pretreatment: None Test conditions: Dilution water: Carbon filtered well water Dilution water chemistry: pH: 84 DO: 8.1 mglL `Stock and test solution preparation: A primary stock solution was prepared in dilution water to yield a test sample concentration of 400 mg/L. All test solutions were made by diluting the appropriate amount of stock solution with dilutionwaterto make 1 L. of solution per concentration. Stability of the test chemical solutions: Not noted. Exposure vessels: 2L glass beakers Number of replicates: two. `Number of fish per replicate: ten 001526 Numberof concentrations: six plus a negative control Water chemistry during the study: Dissolved oxygen range (0 -- 96 hours): 46..08-77..92 gL. mg/L (control exposure) (56.0 mglL exposure) pH range (0 -- 96 hours) 8.0-8.4 (control exposure) 8.0-8.4 (56.0 mg/L exposure) Test temperature range (0 96 hours) 19.2-19.5C RESULTS Nominal concentrations: Bk control, 3.2, 5.6, 10.0, 18.0, 32.0, 56.0 Element value: mglL 96-hour LCso= 19 mg/L (95% C.I.: 16-24 mg/L) Mortality of controls: None Remarks: Values reported are for the test sample. No calculations were made to adjust for the concentrationofthe test substance in the test sample. CONCLUSIONS eTxhheibtietsetdsaam9p6l-ehocuorntLaCisnoinfogr2f4a.th5e%adPemriflnunoorwooocfta1n9esmuglifLo.nate, Lithium salt Submitter: 3M Company, St. Paul, Minnesota, 55133 Environmental Laboratory, P.O: Box 33331, DATA QUALITY aRnealliyatbiiclailtyd:atKaiifomri:scdhetrearnmkiinnagti2o.n oTfhitshestteusdty,suwhbislteanwcelelccoonncdeuncttreatdi,olnaicnksthe test solutions; and determination of the sample purity. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, Lab Request number M1018, 3/25/34. oTHWER Last changed: 5/2/00 OOOOO00 001527 ENVIRONMENTAL LABORATORY LR: M1018 Date: 3/25/94 Analyst: JBD Pimephales promelas 96-hour Toxicity Test Data Summary SAMPLE: FC-94-X (F-11367, Lot #1) `Sample Conc. mg/L 32 5.6 10 18 32 56 ; 24 hours | o 0 0 Percent Mortality 48 hours | 72 hous | 0 o 0 0 0 10 0 50 20 60 96 hours o 5 15 - 50 65 100 24hLC50(95% Cl)= 48hLCSO (95% Cl)= > 56 mol > 56 mol . 72hLC50 (95% C.l)= 36 moll (15-85) 96hLCS0 (95% Cl)= 19 mL (16-24) NOTE: Spearman-Karber method used to calculate those LC50's which could be estimated. (72 h LCSO required 40% trim, 96 h LC50 did not require any trim) 001528 il ENVIRONMENTAL LABORATORY Ww _miog 96-HR ACUTE TOXICITY TO FATHEAD MINNOW, PIMEPHALES PROMELAS TPETEST. STATIC _X_ STATICRENEWAL TESTSUBSTANGE: FC-QU-X (Foun, Lex =D DILUTIONWATER: ~~ CARBON-FIWLELTLa EWRATEEDR(GM W5 ELL#5 2 ST.EPrAUmL, TESTRSHSOURCE: AghaesriCcoBlteosne,teCm0s ac. DATERECEVED: 1-21-Q4 FISH AGE: TG days AVGWT: Oued #039 Ava.SZE: Sa #dem NO.OF FISHTEST CHAMBER: 0 onomarare: ZS AL ana "TESTSOLUTIONVOLUME: IL `SIZEOFTESTVESSELS: QL DATEINTATED: 3-21-Q4 @ 1(:00 DATETERMINATED: 3-35@\-\:9Sc\am ETore f OUT RZm 4RFRAoprHgD0e .[ReWm RMPADOY.a [eKY TeZmRR pH sDO.m[T e HRrPHoB] O. wml [T Te E Gel GC (G Ears6O [E30 ssa| 8 [olsalealo[aluzlnaells[osalleon + [se| w [olealeslo[silealo[ealnolo[sale] 2+ fe[90]o| oVFs E [ol S e [Es salTeaa arlS ogolr Tsaalra easlTA oyl T[anare ltee owe| we [ol[e sils a]o l [sale esEun lssla an] + [sow [olaalaale[ar[malulaalals foals] v 2 [seo| [0] wo [ofs [ala ia]l s [ee aleas liol [aus la] oes Tale TR TeTaTug] . 2 C rrrr rrr rT rT rT , rrrrrrrrr 2 ES Los0(95% G1) Aon Lenn 1a mej (lead mye EUS SPAETERay COMMENTS "E mb rrr staehd aseain.rodci,lT unT tedetYA eoS \LC(wSYietGShmTgd/igih~o>n Bwoaak.es---- `SECONDARYEFFECTS: =Surfacing:E =LossofEQUBum;O = ulescent =EmalioSwimming:H=Femormaging AwLysT: Tu 001529 amie 1.0:mw0 sample Description TO-aw-x Environmental Lab -- Work Sheet Lab Request No. MIO\Y "208 ------ of -- Date: _=F ~QY Analyst: _TDD Deruoinailen fF leche cod & "Coals group Rogsocelas aX Meconlachion of Qb-Mous susuive) assay Ein \ 2 A " Ss G a | 2 Qq BERR Lean (con oelginx (0) 2. ORR 20 SOSIR Lo SOR 2.0 QGUR 27 2 RAEN 22 LOB\O 2.0 SER 23 LORD 220 ~0eCO. SNHY--H--i r";Isti i h.S.TM_ ro0 ps sr--innn X~ a. H~ .063G s.~ 03 SD. on" . - ) eee S63SEe EN * TBRUIRMLMIENDGTOSNFERAERSMEAANR-CFHA.REEIRNC.METHOD FOR CALCULATION OF ECSO AND LCSC VALLES IN BIGASSAVS 3 4F.O0R. REHFAEMRIELNTCOEN.. RC.ICT.E RUSSO. AND R.V. THURSTON. 1977. LTERTIHMAMELDCOSNPCEEANRTNRAANT-IKOANRSSZRINMETTOHXOIDCITFYORBIEGSATGISMAAYTSI.NG MEDIAN CENOVRIRREOCNT.IONSCI1.2(4T)ECH4N1O7L. (1113(778)1. 714-71% DTAETSET _ i3/02118/94 sCPHzEcMiIzsCAL. >F.o-gpar-oxmsias DURATION, 304DATA 52 hours NCEUNMCBEENRTREAXTPIOOSNEGDmG/L) NMORTALITIES J3.z20 "5 5.6200 10.2000 18.2000 32.0200 1 3 to 13 SSPPEEAARRMMAANN--KKAARREEEERR ETRSITMIMATES eso 0.00 19.4886284 . 9755%% ULFOFWEERR CCOONNFFIIDDEENNCCEE 2145..0603 mioig 56.5200 20 001531 .. . 5 3 3 3 . 3.015 v N 3 3 mow Al hous 3ESTCOPY AVAILABLE SURLINGTON RESERRCH. INC. ; " " 3. :5 i 1.006 5 - i3 . 5 i DDDDDEDDDODLDDE DDDDOIDDDDDIDDEDDDIDDDDTODDDDEDDDDDDDIDODDODE 001532 S- rate TESTE _Sriozt/ae4 * SCPHEECMIIECSAL FFPo. -9o4r-oxmelas RaSUlRADToIOTN. 72 hours NCOUNMCBEENRTREAXTPIOOSNED(mg/L) MORTALITIES 3.2200 5.6200 10.2005 18.2000 32.0200 2 10 SSPPEEAARRMMAANN--KKAARRBBEERR TERSITMIMATES Leso40.00 35.5052032 9955%7 LLPOFWEERR CCOONNFFIIDDEENNCCEE 344..883 mioig 56.0200 12 001533 ee Miog 4% woos BESTCOPY AVAILABLE ; : $7;i3 ~~ hd 3 ae HNi IE3ed . Po 3 :: i:: 3:: ; Xx ;. 001534