Document YjKzvKwo4ooRvv7xKvwNooNq0

ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity:Perfluorooctanoiaccid,ammonium salt;may also be referredto as PFOA ammonium salt,Ammonium perfluorooctanoateP,FO, FC116, FC-126, FC-169, or FC-143. (Octanoicacid,pentadecafluoroammonium salt,CAS # 3825-26-1) Remarks: The 3M productionlotnumber was 83.The testsample isFC143. It'psuritywas notsufficientclhyaracterizedt,hough currentinformation indicatesitisa mixtureof96.5 - 100% testsubstance and 0 - 3.5% C6, C7, and Cg perfluoroanalogue compounds. METHOD: Method: Not noted. Type: Staticacute GLP: No Year completed: 1978 Species: Bluegilslunfish(Lepomismachrochinis) Supplier:Not noted. Analyticalmonitoring:Temperature,pH and DO Exposure period:96-hours Statisticamlethods: Not noted Testfishage: Not noted. Length and weight: Average length 4 cm Average weight 0.20 g Pretreatment: Not noted. Test conditions: ' Dilutionwater: Carbon-filterewdellwater. Dilutionwater chemistry: Temperature: 19*C Dissolved Oxygen: 9.1 ppm pH: 7.9 Stock and testsolutionpreparation:Not noted Concentrations dosing rate:Once Stabilityof the testchemical solutions:Not noted Exposure vessels: Tanks containing16 litertsestsolution. Number of replicates:one Number of fishper replicate:20 Loading rate:0.25 g/L Number of concentrations:fiveplusa blankcontrol Water chemistry during the study: Dissolved oxygen range (24- 96 hours): 5.3- 6.9 mg/L (controlexposure) 5.1- 7.3 mg/L (420 mg/L exposure) pH range (24 - 96 hours): 7.9 (controelxposure) 7.8- 8.0 (420 mg/L exposure) Test temperature (24- 96 hours):18-190C RESULTS Nominal concentrations: Bk control1,35,180, 240, 320,and 420 mg/L Element value: 96-hour LC50 = >420 mg/L Element valuebased on nominal concentrations CONCLUSIONS The testsample 96-hour LC5o forbluegilslunfishwas determined tobe >420 mg/L, the highestconcentrationtested. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilitKyl:imischranking3. Testinglacksinformatioonn the method followed.No informatioonn stockortestsolutionpreparations.Average fish weight issuspect.Sample puritywas not properlycharacte(izeadnd itlacks analyticaclonfirmationoftestsubstance concentrations. REFERENCES This studywas conducted by the3M Company, EnvironmentalLaboratory, St.Paul,MN, Lab Request number 3844. OTHER Last changed: 5/24/00 ENVIRONMENTAL ENGINEERING LABORATORY AQUATIC TOXICITY DATA SHEET Type Test 0 @lf Material Tested Test Organism Avg. Wt.0.2,:,Srams Avg. Size cm Test Solution Volume Liters/Tank No. Organisms per dose O;kl(3 Time Started Date Started Date Completed Dilution Water Carbon Filter Well Water (Temp. ICI OC. D. O.C:L ppm. pH Investigator LC 50 (9S% Confidence Limits) No. rgaiiisms 24 Hrs. Conc. @xposed No.Dead F@k Con--tr-o.I @,<:) o OA(5 ';L,(3 (3 t-0A C-) 0.041 4880. tirs. L)0. pli ppm L No. Dead __Lli p;m 72 lirs. D.O. 96 Hrs. D.G. No. Dead pfi_ ppm iNo.Dead. PH -jlu i @.P@I(b.1 C) C) -7q ci ic@ r C) -7q -ko Avg. Temp. Comments: